MD02G1124200.v1.1

Calcium-dependent channel, 7TM region, putative phosphate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0015059
Physical Location & Seq
Forward (+)
10122467 .. 10124911
2445 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1124200.v1.1.491

Sequence Viewer

Length: 2445 bp
ATGGCCACGCCTTATCAATCTTCAGTCTTCTCCCCAATACCCTCCATGAACGACACTCTCTCTCCTCCGCCGTCCCCCGTCGACGGCGCCGATACCTGCGAGGCTTGGTACGGCAACATCCAGTACCTCCTCAACATCTCCGCCATCGGCTCCTTCTTCTGCGTCTTCTTCTTCATATTTGTCAAGCTTCGCAGCGACCACCGCCGCATGCCCGGTCCGTCTGCCCTGGTCGCTAAGCTCCTTGCGGTGTGGCACGCCACGTGCCGTGAGATTGCGCGCCACTGCGGCGCTGACGCCGCCCAGTTTCTCTTGATCGAGGGCGGCAGCTGCGGGTTGCTATTGTCCATGGCGGTTTTGGCTGTGCTTGTAATGCTTCCGCTGAATCTCTACGCTGGGAGTGCTGTGCTGGGGGATCAATTCTCGAAGACGACCATCAATCACATTGAGAAAGGTTCGGCTTTGCTTTGGGTGCATTTCGTTTTCGTGGTTGTTGTTGTTGTTCTTGTGCATTTTGGTATTTCTGCTATTGAAGGGAGATTGAGAATCACTAGGATTAGGGATGTGAATGGCAATTTGAGTGACCCGACTGTGAATTCCATGGCGATTTTTACTATAATGGTGCAGGGGATACCGAAAACCATAGGGAATGATGGGACTGTGCTGCATGAGTATTTTCAACATAGGTATCCTGGAAAGGTTTATAGGGTTATAATGCCGATGGATTTGTGTGCTTTGGATGAATTAGCCTCGGAATTAGTGAGGGTTAGGCATGAAATTTCTTGGTTGGTTGCACGAATTGACTCTCGGCTCTTGCCGTATGAAAGTGAAGAGAATGGATACGTAAGGACTTCTTCAGAGGGAGTATGGGGATGTGCTTGTAATTTATGGCGAAAGGTAGAGGATTTCTGGTATCATACTATGGCTAGGTTGGGATACACCGATGAAAGGAAGTTAGGAGAGTTGCAGGGATGGAGAGCTGAATTGGAGACTGAACTGGCAGCTTACAAAGAAGGTCGTGCACTAGGTGCTGGAGTTGCATTTGTGGTGTTTAAGGATGTGTACACTGCTAATAAAGCGGTTCAGGACTTTCGACATGAGAAGAAGAGGCGGATAGGGAAATTCTTTTCTCTTGTGGAATTGCGTTTACAGAGAAACCAATGGAAAGTTGAGCAGGCACCCTTGGCCACGGATATTTATTGGAATCATTTGGGGTCGTCGAAGCTCTCACTGAAATTGCGAAGAGTGTTGGTGAACACGTGCTTGTTGTTGATTCTTCTGTTCTTTAGCTCTCCTCTGGCAATTATCAGTGCTCTAAAAAATGCCTGGCGGATTATAAATGCGGAAGCTATGGATAACGCCCAGTTGTGGTTTGCTTGGGTGCAGAGCTCAAGCTGGCTCGGGAGTCTTATCTTTCAGTTTCTACCCAATGTGTTTATTTTTGTCAGCATGTATATGGTTATTCCATCAGCACTTTCATATCTCTCCAAGTTCGAGCGACATCTTACAGTGTCGGGGGAGCAACGAGCTGCTCTTCTTAAGATGGTTTGTTTCTTCCTGGTGAATCTTATACTTCTTAAGGGACTGGTTGAATCATCTTTAGAGAGTGCCATCTTAAAAATGGGAAGGTGCTATATGGATGGAGAAGATTGCAAGAGAATTGAGCAGTACATGAGTGCCTCGTTTTTGTCAAGATCGTGTCTTTCTTCTCTTGCATTCTTGATAACAAGTACATTCCTGGGCATATCTTATGATCTATTGGCTCCAATCCCTTGGATAAAAAGGAAGATTCAGAAGTTTCGGAAGAATGACATGCTACAGCTAGTCCCGGAACAAAGTGAAGAATACGCATTGGAAACTCAGGAAACAAATGGTCTAGAGAGGCCCTTGATTGTGGACAACACTTATGATTCTCCCAGAACTCTGCAGGGAAATGATCTCTCTGATTACCCAATCAACAGAACATCAACTGCCCCCAAACAGACATTTGATTTCGCCCAATATTATGCATTCAATCTGACGATATTTTCCCTGACCTTCATATATTCTTCCTTCTCCCCACTTGTGGTTCCCGTTGGTGCTATTTATTTTGGGTATAGGTACCTGGTCGACAAGTACAACTTCCTCTTTGTCTATAGGGTCCGGGGCTTTCCTGCTGGGAATGATGGGAAGTTGATGGATACAGTTCTGTGCATCATGCGTTTTTGCGTCGATTTATTCCTGCTGGCAATGCTGTTGTTCTTTTCGGTCCACGGAGACTCAACGAAGCTGCAAGCTATATTCACACTTGGGGTATTAGTAATGTATAAATTGTTGCCTTCCCATAATGACTGTCTTCATCCAGCTCTTTTGGAAGGCGTACAGACTGTAGACAGTGTTGTGGATGGACCTATAGATTATGAGGTGTATTCACAGCCCAAGTTTGATTGGGATACATATTATTCATGA

Protein Analysis

815

Amino Acids

92.26

Weight (kDa)

7.21

Isoelectric Point (pI)

42.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 66 - 172 3e-11 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 203 - 402 8.9e-12 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 413 - 598 5.9e-11 Calcium-dependent channel, 7TM region, putative phosphate
RSN1_7TM PF02714 659 - 747 5.2e-12 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015059)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35870
fragaria_vesca FvH4_1g11170
malus_domestica MD02G1124200.v1.1 MD15G1238200.v1.1
prunus_persica Prupe.7G174200_v2.0.a1
pyrus_communis pycom02g09710 pycom05g15420 pycom15g21190
rosa_chinensis RchiOBHm_Chr2g0098461
rosa_laevigata RLG00000016800
rosa_multiflora Rmu_sc0010938.1_g000006
rosa_roxburghii Rroxscaffold_2G00143940
rosa_rugosa Rorug02G0073400
rosa_samantha Rh2AG120700 Rh2DG125700
rosa_wichuraiana Rw2G009460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 710, 1334
Acc36I ACCTGC 1 cut(s) 104
Acc65I GGTACC 1 cut(s) 2097
AccB1I GGYRCC 3 cut(s) 86, 1174, 2097
AccI GTMKAC 3 cut(s) 81, 2106, 2367
AccII CGCG 1 cut(s) 277
AclWI GGATC 1 cut(s) 420
AcoI YGGCCR 2 cut(s) 3, 1182
AcsI RAATTY 3 cut(s) 592, 774, 1118
AcuI CTGAAG 2 cut(s) 6, 837
AcvI CACGTG 2 cut(s) 261, 1257
AcyI GRCGYC 2 cut(s) 87, 294
AdeI CACNNNGTG 1 cut(s) 1025
AfaI GTAC 8 cut(s) 110, 125, 1061, 1667, 1729, 2099, 2114, 2358
AfiI CCNNNNNNNGG 4 cut(s) 83, 945, 1365, 2062
AflII CTTAAG 2 cut(s) 1535, 1574
AflIII ACRYGT 1 cut(s) 1254
AgsI TTSAA 4 cut(s) 530, 677, 1589, 2011
AjnI CCWGG 6 cut(s) 225, 688, 1322, 1554, 1734, 2100
Alw21I GWGCWC 3 cut(s) 1021, 1312, 1388
Alw26I GTCTC 2 cut(s) 980, 2247
Alw44I GTGCAC 1 cut(s) 1017
AlwI GGATC 1 cut(s) 420
Ama87I CYCGRG 1 cut(s) 1397
AoxI GGCC 3 cut(s) 3, 1182, 1880
ApaLI GTGCAC 1 cut(s) 1017
ApeKI GCWGC 7 cut(s) 192, 324, 327, 661, 998, 1526, 2266
ApoI RAATTY 3 cut(s) 592, 774, 1118
Asp718I GGTACC 1 cut(s) 2097
AspLEI GCGC 4 cut(s) 89, 277, 279, 290
AspS9I GGNCC 5 cut(s) 215, 1881, 2137, 2245, 2384
AsuC2I CCSGG 3 cut(s) 213, 1826, 2141
AsuHPI GGTGA 2 cut(s) 1261, 1570
AvaI CYCGRG 1 cut(s) 1397
AvaII GGWCC 4 cut(s) 215, 2137, 2245, 2384
BaeGI GKGCMC 1 cut(s) 1021
BaeI ACNNNNGTAYC 2 cut(s) 668, 701
BalI TGGCCA 2 cut(s) 5, 1184
BanI GGYRCC 3 cut(s) 86, 1174, 2097
BanII GRGCYC 1 cut(s) 1388
BbrPI CACGTG 2 cut(s) 261, 1257
BbsI GAAGAC 4 cut(s) 19, 157, 431, 2324
Bbv12I GWGCWC 3 cut(s) 1021, 1312, 1388
BbvI GCAGC 7 cut(s) 204, 314, 336, 648, 1010, 1513, 2253
BceAI ACGGC 5 cut(s) 55, 100, 127, 249, 799
BciT130I CCWGG 6 cut(s) 227, 690, 1324, 1556, 1736, 2102
BciVI GTATCC 6 cut(s) 621, 696, 830, 926, 2170, 2422
BcnI CCSGG 3 cut(s) 213, 1826, 2141
BcoDI GTCTC 2 cut(s) 980, 2247
BfaI CTAG 5 cut(s) 549, 924, 1022, 1820, 1874
BfmI CTRYAG 5 cut(s) 1814, 1922, 2131, 2364, 2388
BfoI RGCGCY 2 cut(s) 90, 291
BfrI CTTAAG 2 cut(s) 1535, 1574
BfuAI ACCTGC 1 cut(s) 104
BfuI GTATCC 6 cut(s) 621, 696, 830, 926, 2170, 2422
BglI GCCNNNNNGGC 1 cut(s) 285
BlpI GCTNAGC 1 cut(s) 234
Bme1390I CCNGG 9 cut(s) 213, 227, 690, 1324, 1556, 1736, 1826, 2102, 2141
Bme18I GGWCC 4 cut(s) 215, 2137, 2245, 2384
BmeT110I CYCGRG 1 cut(s) 1397
BmgT120I GGNCC 5 cut(s) 215, 1881, 2137, 2245, 2384
BmiI GGNNCC 7 cut(s) 88, 151, 1176, 1761, 2067, 2099, 2138
BmrFI CCNGG 9 cut(s) 213, 227, 690, 1324, 1556, 1736, 1826, 2102, 2141
BmrI ACTGGG 2 cut(s) 295, 1354
BmsI GCATC 1 cut(s) 2199
BmuI ACTGGG 2 cut(s) 295, 1354
BpiI GAAGAC 4 cut(s) 19, 157, 431, 2324
BpmI CTGGAG 1 cut(s) 1050
Bpu1102I GCTNAGC 1 cut(s) 234
BpuEI CTTGAG 1 cut(s) 1372
BpuMI CCSGG 3 cut(s) 213, 1826, 2141
BsaAI YACGTR 3 cut(s) 261, 841, 1257
BsaHI GRCGYC 2 cut(s) 87, 294
BsaXI ACNNNNNCTCC 2 cut(s) 46, 76
Bsc4I CCNNNNNNNGG 4 cut(s) 83, 945, 1365, 2062
Bse1I ACTGG 5 cut(s) 121, 301, 999, 1360, 1587
Bse3DI GCAATG 1 cut(s) 2232
BseBI CCWGG 6 cut(s) 227, 690, 1324, 1556, 1736, 2102
BseGI GGATG 9 cut(s) 117, 565, 742, 875, 974, 1060, 1642, 2335, 2386
BseLI CCNNNNNNNGG 4 cut(s) 83, 945, 1365, 2062
BseMI GCAATG 1 cut(s) 2232
BseMII CTCAG 1 cut(s) 1871
BseNI ACTGG 5 cut(s) 121, 301, 999, 1360, 1587
BsePI GCGCGC 1 cut(s) 275
BseRI GAGGAG 3 cut(s) 54, 119, 1281
BseSI GKGCMC 1 cut(s) 1021
BseXI GCAGC 7 cut(s) 204, 314, 336, 648, 1010, 1513, 2253
BseYI CCCAGC 3 cut(s) 392, 406, 2153
BsgI GTGCAG 2 cut(s) 641, 1400
Bsh1236I CGCG 1 cut(s) 277
BshFI GGCC 3 cut(s) 5, 1184, 1882
BshNI GGYRCC 3 cut(s) 86, 1174, 2097
BsiHKAI GWGCWC 3 cut(s) 1021, 1312, 1388
BsiHKCI CYCGRG 1 cut(s) 1397
BsiSI CCGG 3 cut(s) 213, 1826, 2140
BslFI GGGAC 4 cut(s) 58, 667, 1593, 1808
BslI CCNNNNNNNGG 4 cut(s) 83, 945, 1365, 2062
BsmAI GTCTC 2 cut(s) 980, 2247
BsmFI GGGAC 4 cut(s) 58, 667, 1593, 1808
BsmI GAATGC 2 cut(s) 1712, 2006
BsnI GGCC 3 cut(s) 5, 1184, 1882
BsoBI CYCGRG 1 cut(s) 1397
Bsp1286I GDGCHC 3 cut(s) 1021, 1312, 1388
Bsp1407I TGTACA 1 cut(s) 1059
Bsp143I GATC 5 cut(s) 312, 412, 1691, 1750, 1933
Bsp1720I GCTNAGC 1 cut(s) 234
Bsp19I CCATGG 2 cut(s) 345, 597
BspANI GGCC 3 cut(s) 5, 1184, 1882
BspCNI CTCAG 1 cut(s) 1870
BspFNI CGCG 1 cut(s) 277
BspHI TCATGA 1 cut(s) 2441
BspLI GGNNCC 7 cut(s) 88, 151, 1176, 1761, 2067, 2099, 2138
BspMAI CTGCAG 1 cut(s) 1926
BspMI ACCTGC 1 cut(s) 104
BspPI GGATC 1 cut(s) 420
BspQI GCTCTTC 1 cut(s) 1536
BspT107I GGYRCC 3 cut(s) 86, 1174, 2097
BspTI CTTAAG 2 cut(s) 1535, 1574
BsrDI GCAATG 1 cut(s) 2232
BsrGI TGTACA 1 cut(s) 1059
BsrI ACTGG 5 cut(s) 121, 301, 999, 1360, 1587
BssHII GCGCGC 1 cut(s) 275
BssMI GATC 5 cut(s) 312, 412, 1691, 1750, 1933
BssNI GRCGYC 2 cut(s) 87, 294
BssT1I CCWWGG 4 cut(s) 345, 597, 1179, 1769
Bst2UI CCWGG 6 cut(s) 227, 690, 1324, 1556, 1736, 2102
Bst4CI ACNGT 7 cut(s) 589, 658, 1507, 2182, 2330, 2365, 2372
Bst6I CTCTTC 4 cut(s) 822, 1097, 1234, 1536
BstACI GRCGYC 2 cut(s) 87, 294
BstAFI CTTAAG 2 cut(s) 1535, 1574
BstAPI GCANNNNNTGC 1 cut(s) 1025
BstAUI TGTACA 1 cut(s) 1059
BstBAI YACGTR 3 cut(s) 261, 841, 1257
BstC8I GCNNGC 7 cut(s) 209, 255, 277, 1173, 1394, 2223, 2271
BstDEI CTNAG 2 cut(s) 234, 1857
BstDSI CCRYGG 4 cut(s) 345, 597, 1185, 2248
BstF5I GGATG 9 cut(s) 117, 565, 742, 875, 974, 1060, 1642, 2335, 2386
BstFNI CGCG 1 cut(s) 277
BstH2I RGCGCY 2 cut(s) 90, 291
BstHHI GCGC 4 cut(s) 89, 277, 279, 290
BstKTI GATC 5 cut(s) 315, 415, 1694, 1753, 1936
BstMAI GTCTC 2 cut(s) 980, 2247
BstMBI GATC 5 cut(s) 312, 412, 1691, 1750, 1933
BstNI CCWGG 6 cut(s) 227, 690, 1324, 1556, 1736, 2102
BstNSI RCATGY 3 cut(s) 211, 1450, 1813
BstSCI CCNGG 9 cut(s) 211, 225, 688, 1322, 1554, 1734, 1824, 2100, 2139
BstSFI CTRYAG 5 cut(s) 1814, 1922, 2131, 2364, 2388
BstSLI GKGCMC 1 cut(s) 1021
BstSNI TACGTA 1 cut(s) 841
BstUI CGCG 1 cut(s) 277
BstV1I GCAGC 7 cut(s) 204, 314, 336, 648, 1010, 1513, 2253
BstV2I GAAGAC 4 cut(s) 19, 157, 431, 2324
BstXI CCANNNNNNTGG 1 cut(s) 1770
BsuI GTATCC 6 cut(s) 621, 696, 830, 926, 2170, 2422
BsuRI GGCC 3 cut(s) 5, 1184, 1882
BtgI CCRYGG 4 cut(s) 345, 597, 1185, 2248
BtsCI GGATG 9 cut(s) 117, 565, 742, 875, 974, 1060, 1642, 2335, 2386
BtsI GCAGTG 2 cut(s) 280, 1062
BtsIMutI CAGTG 6 cut(s) 280, 1062, 1226, 1312, 1512, 2377
BveI ACCTGC 1 cut(s) 104
Cac8I GCNNGC 7 cut(s) 209, 255, 277, 1173, 1394, 2223, 2271
CciI TCATGA 1 cut(s) 2441
CfoI GCGC 4 cut(s) 89, 277, 279, 290
Cfr13I GGNCC 5 cut(s) 215, 1881, 2137, 2245, 2384
CpoI CGGWCCG 1 cut(s) 215
CseI GACGC 3 cut(s) 151, 302, 2194
CsiI ACCWGGT 1 cut(s) 2100
Csp6I GTAC 8 cut(s) 109, 124, 1060, 1666, 1728, 2098, 2113, 2357
CspI CGGWCCG 1 cut(s) 215
CviQI GTAC 8 cut(s) 109, 124, 1060, 1666, 1728, 2098, 2113, 2357
DdeI CTNAG 2 cut(s) 234, 1857
DinI GGCGCC 1 cut(s) 88
DpnI GATC 5 cut(s) 314, 414, 1693, 1752, 1935
DpnII GATC 5 cut(s) 312, 412, 1691, 1750, 1933
DraIII CACNNNGTG 1 cut(s) 1025
EaeI YGGCCR 2 cut(s) 3, 1182
Eam1104I CTCTTC 4 cut(s) 822, 1097, 1234, 1536
EarI CTCTTC 4 cut(s) 822, 1097, 1234, 1536
EciI GGCGGA 4 cut(s) 57, 130, 1123, 1342
Ecl136II GAGCTC 1 cut(s) 1386
Eco105I TACGTA 1 cut(s) 841
Eco130I CCWWGG 4 cut(s) 345, 597, 1179, 1769
Eco24I GRGCYC 1 cut(s) 1388
Eco47I GGWCC 4 cut(s) 215, 2137, 2245, 2384
Eco53kI GAGCTC 1 cut(s) 1386
Eco57I CTGAAG 2 cut(s) 6, 837
Eco72I CACGTG 2 cut(s) 261, 1257
Eco88I CYCGRG 1 cut(s) 1397
EcoICRI GAGCTC 1 cut(s) 1386
EcoO109I RGGNCCY 1 cut(s) 1881
EcoRI GAATTC 1 cut(s) 592
EcoRII CCWGG 6 cut(s) 225, 688, 1322, 1554, 1734, 2100
EcoT14I CCWWGG 4 cut(s) 345, 597, 1179, 1769
EcoT22I ATGCAT 1 cut(s) 2008
EcoT38I GRGCYC 1 cut(s) 1388
EgeI GGCGCC 1 cut(s) 88
EheI GGCGCC 1 cut(s) 88
ErhI CCWWGG 4 cut(s) 345, 597, 1179, 1769
FalI AAGNNNNNCTT 4 cut(s) 835, 867, 2102, 2134
FaqI GGGAC 4 cut(s) 58, 667, 1593, 1808
FauI CCCGC 1 cut(s) 323
FblI GTMKAC 3 cut(s) 81, 2106, 2367
FokI GGATG 9 cut(s) 104, 572, 749, 882, 981, 1067, 1649, 2322, 2393
FriOI GRGCYC 1 cut(s) 1388
FspBI CTAG 5 cut(s) 549, 924, 1022, 1820, 1874
GlaI GCGC 4 cut(s) 88, 276, 278, 289
GsaI CCCAGC 3 cut(s) 396, 410, 2157
GsuI CTGGAG 1 cut(s) 1050
HaeII RGCGCY 2 cut(s) 90, 291
HaeIII GGCC 3 cut(s) 5, 1184, 1882
HapII CCGG 3 cut(s) 213, 1826, 2140
HgaI GACGC 3 cut(s) 151, 302, 2194
HhaI GCGC 4 cut(s) 89, 277, 279, 290
Hin1I GRCGYC 2 cut(s) 87, 294
Hin6I GCGC 4 cut(s) 87, 275, 277, 288
HinP1I GCGC 4 cut(s) 87, 275, 277, 288
HincII GTYRAC 2 cut(s) 82, 2107
HindII GTYRAC 2 cut(s) 82, 2107
HindIII AAGCTT 1 cut(s) 185
HpaII CCGG 3 cut(s) 213, 1826, 2140
HphI GGTGA 2 cut(s) 1261, 1570
Hpy188I TCNGA 6 cut(s) 751, 856, 1791, 1800, 1942, 2016
Hpy188III TCNNGA 9 cut(s) 310, 421, 1082, 1399, 1689, 1717, 1859, 1874, 2442
Hpy99I CGWCG 4 cut(s) 83, 86, 1219, 2210
HpyAV CCTTC 8 cut(s) 163, 524, 1004, 1617, 2044, 2059, 2325, 2345
HpyCH4III ACNGT 7 cut(s) 589, 658, 1507, 2182, 2330, 2365, 2372
HpyCH4IV ACGT 3 cut(s) 260, 840, 1256
HpyF3I CTNAG 2 cut(s) 234, 1857
HpySE526I ACGT 3 cut(s) 260, 840, 1256
Hsp92I GRCGYC 2 cut(s) 87, 294
HspAI GCGC 4 cut(s) 87, 275, 277, 288
KasI GGCGCC 1 cut(s) 86
KpnI GGTACC 1 cut(s) 2101
Kzo9I GATC 5 cut(s) 312, 412, 1691, 1750, 1933
LguI GCTCTTC 1 cut(s) 1536
LmnI GCTCC 4 cut(s) 155, 243, 1516, 1765
Lsp1109I GCAGC 7 cut(s) 204, 314, 336, 648, 1010, 1513, 2253
LweI GCATC 1 cut(s) 2199
MabI ACCWGGT 1 cut(s) 2100
MaeI CTAG 5 cut(s) 549, 924, 1022, 1820, 1874
MaeII ACGT 3 cut(s) 260, 840, 1256
MaeIII GTNAC 1 cut(s) 578
MalI GATC 5 cut(s) 314, 414, 1693, 1752, 1935
MboI GATC 5 cut(s) 312, 412, 1691, 1750, 1933
MhlI GDGCHC 3 cut(s) 1021, 1312, 1388
MlsI TGGCCA 2 cut(s) 5, 1184
MluNI TGGCCA 2 cut(s) 5, 1184
Mly113I GGCGCC 1 cut(s) 87
MlyI GAGTC 3 cut(s) 794, 1411, 2249
Mox20I TGGCCA 2 cut(s) 5, 1184
Mph1103I ATGCAT 1 cut(s) 2008
MscI TGGCCA 2 cut(s) 5, 1184
MseI TTAA 4 cut(s) 1050, 1536, 1575, 1613
MslI CAYNNNNRTG 1 cut(s) 1451
Msp20I TGGCCA 2 cut(s) 5, 1184
MspA1I CMGCKG 2 cut(s) 327, 379
MspCI CTTAAG 2 cut(s) 1535, 1574
MspI CCGG 3 cut(s) 213, 1826, 2140
MspR9I CCNGG 9 cut(s) 213, 227, 690, 1324, 1556, 1736, 1826, 2102, 2141
Mva1269I GAATGC 2 cut(s) 1712, 2006
MvaI CCWGG 6 cut(s) 227, 690, 1324, 1556, 1736, 2102
MvnI CGCG 1 cut(s) 277
NarI GGCGCC 1 cut(s) 87
NciI CCSGG 3 cut(s) 213, 1826, 2141
NcoI CCATGG 2 cut(s) 345, 597
NdeII GATC 5 cut(s) 312, 412, 1691, 1750, 1933
NlaIV GGNNCC 7 cut(s) 88, 151, 1176, 1761, 2067, 2099, 2138
NmeAIII GCCGAG 1 cut(s) 784
NmuCI GTSAC 1 cut(s) 578
NsiI ATGCAT 1 cut(s) 2008
NspI RCATGY 3 cut(s) 211, 1450, 1813
PaeI GCATGC 1 cut(s) 211
PagI TCATGA 1 cut(s) 2441
PauI GCGCGC 1 cut(s) 275
PciSI GCTCTTC 1 cut(s) 1536
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PctI GAATGC 2 cut(s) 1712, 2006
PfeI GAWTC 8 cut(s) 382, 543, 1201, 1270, 1561, 1589, 1786, 1907
PfoI TCCNGGA 2 cut(s) 688, 1824
PleI GAGTC 3 cut(s) 794, 1410, 2249
PluTI GGCGCC 1 cut(s) 90
PmaCI CACGTG 2 cut(s) 261, 1257
PmlI CACGTG 2 cut(s) 261, 1257
PpsI GAGTC 3 cut(s) 794, 1410, 2249
Ppu21I YACGTR 3 cut(s) 261, 841, 1257
PsiI TTATAA 2 cut(s) 710, 1334
Psp124BI GAGCTC 1 cut(s) 1388
Psp6I CCWGG 6 cut(s) 225, 688, 1322, 1554, 1734, 2100
PspCI CACGTG 2 cut(s) 261, 1257
PspFI CCCAGC 3 cut(s) 392, 406, 2153
PspGI CCWGG 6 cut(s) 225, 688, 1322, 1554, 1734, 2100
PspN4I GGNNCC 7 cut(s) 88, 151, 1176, 1761, 2067, 2099, 2138
PspPI GGNCC 5 cut(s) 215, 1881, 2137, 2245, 2384
PstI CTGCAG 1 cut(s) 1926
PteI GCGCGC 1 cut(s) 275
PvuII CAGCTG 1 cut(s) 327
RsaI GTAC 8 cut(s) 110, 125, 1061, 1667, 1729, 2099, 2114, 2358
RsaNI GTAC 8 cut(s) 109, 124, 1060, 1666, 1728, 2098, 2113, 2357
RseI CAYNNNNRTG 1 cut(s) 1451
Rsr2I CGGWCCG 1 cut(s) 215
RsrII CGGWCCG 1 cut(s) 215
SacI GAGCTC 1 cut(s) 1388
SalI GTCGAC 2 cut(s) 80, 2105
SapI GCTCTTC 1 cut(s) 1536
SaqAI TTAA 4 cut(s) 1050, 1536, 1575, 1613
Sau3AI GATC 5 cut(s) 312, 412, 1691, 1750, 1933
Sau96I GGNCC 5 cut(s) 215, 1881, 2137, 2245, 2384
SchI GAGTC 3 cut(s) 794, 1411, 2249
ScrFI CCNGG 9 cut(s) 213, 227, 690, 1324, 1556, 1736, 1826, 2102, 2141
SduI GDGCHC 3 cut(s) 1021, 1312, 1388
SexAI ACCWGGT 1 cut(s) 2100
SfaNI GCATC 1 cut(s) 2199
SfcI CTRYAG 5 cut(s) 1814, 1922, 2131, 2364, 2388
SfoI GGCGCC 1 cut(s) 88
SgrDI CGTCGACG 1 cut(s) 80
SinI GGWCC 4 cut(s) 215, 2137, 2245, 2384
SmiMI CAYNNNNRTG 1 cut(s) 1451
SmlI CTYRAG 3 cut(s) 1387, 1535, 1574
SmoI CTYRAG 3 cut(s) 1387, 1535, 1574
SnaBI TACGTA 1 cut(s) 841
SphI GCATGC 1 cut(s) 211
SspDI GGCGCC 1 cut(s) 86
SspI AATATT 1 cut(s) 2000
SspMI CTAG 5 cut(s) 549, 924, 1022, 1820, 1874
SstI GAGCTC 1 cut(s) 1388
StyD4I CCNGG 9 cut(s) 211, 225, 688, 1322, 1554, 1734, 1824, 2100, 2139
StyI CCWWGG 4 cut(s) 345, 597, 1179, 1769
TaaI ACNGT 7 cut(s) 589, 658, 1507, 2182, 2330, 2365, 2372
TaiI ACGT 3 cut(s) 263, 843, 1259
TaqI TCGA 8 cut(s) 81, 315, 422, 1090, 1217, 1491, 2106, 2208
TaqII GACCGA 1 cut(s) 2233
TatI WGTACW 4 cut(s) 1059, 1665, 1727, 2112
TauI GCSGC 4 cut(s) 207, 288, 299, 324
TfiI GAWTC 8 cut(s) 382, 543, 1201, 1270, 1561, 1589, 1786, 1907
Tru1I TTAA 4 cut(s) 1050, 1536, 1575, 1613
Tru9I TTAA 4 cut(s) 1050, 1536, 1575, 1613
TscAI CASTG 6 cut(s) 287, 1069, 1233, 1312, 1512, 2377
TseFI GTSAC 1 cut(s) 578
TseI GCWGC 7 cut(s) 192, 324, 327, 661, 998, 1526, 2266
Tsp45I GTSAC 1 cut(s) 578
TspGWI ACGGA 3 cut(s) 207, 1202, 2265
TspRI CASTG 6 cut(s) 287, 1069, 1233, 1312, 1512, 2377
Vha464I CTTAAG 2 cut(s) 1535, 1574
VneI GTGCAC 1 cut(s) 1017
VpaK11BI GGWCC 4 cut(s) 215, 2137, 2245, 2384
XapI RAATTY 3 cut(s) 592, 774, 1118
XbaI TCTAGA 1 cut(s) 1873
XceI RCATGY 3 cut(s) 211, 1450, 1813
XcmI CCANNNNNNNNNTGG 2 cut(s) 352, 1615
XmiI GTMKAC 3 cut(s) 81, 2106, 2367
XspI CTAG 5 cut(s) 549, 924, 1022, 1820, 1874
Zsp2I ATGCAT 1 cut(s) 2008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.