Rw2G009460

Calcium-dependent channel, 7TM region, putative phosphate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0015059
Physical Location & Seq
Forward (+)
9563339 .. 9566496
3158 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G009460.1

Sequence Viewer

Length: 2409 bp
ATGAACGACACTCTCTCTCCTCCGCCGTCCCCCGGCGATGGCGACTACGCCGCCTGGTACGGCAACATCCAGTACCTCCTCAACATCTCTGCCATCGGCTCCTTCTTCTGCCTCTTCATCTTCCTCTTCGTCAAGCTCCGCTCCGACCACCGCCGCATGCCGGGACCCTCAGCCCTCGCAGCCAAGCTCCTCGCCGTCTGGCACGCCACTGGCCGCGAAATAGCCCGCCACTGCGGCGCCGACGCCGCCCAATTTCTCCTAATCGAAGGCGGCAGCTGCGGCCTGCTGCTCTCCCTCGCCGTCCTCGCCGTGCTGGTCATGCTTCCGTTGAATCTCTACGCCGGGACGGCCGTGCTCGGCGATCAATTCTCGCAGACTACGATAAACCATATCGAGAAAGGTTCAGCTTTACTTTGGGTGCATTTTGTTTTTCTGGTTGTGGTTGTTGTTTTCGTGCATTTTGGTATTTCTGCTGTAGAGTCAAGGTTGAAGATCACTAGGATTAGGGATGGGAATGGGAATATGAGTGACCCAGGTTCGGATTCCACAGCTCTTTTTACTATAATGGTGCAGGGGATACCGAAAACTATAGGGAGTGATGGGACTGTGTTGCATGAGTATTTTCAGCATAAGTATCCTGGCAAGGTTTATAAGGTTGTTTTGCCAATGGATTTGTGTGCATTGGAAGAATTAGCGTCGGAGTTGGTTAAGATTAGGCATGAAATTGCTTGGTTGGTTGCGAAAATTGACTCCCGGCTCTTGCCTGATGAGAGTATAGAAAATGGATATGGACCGGCTTTGTCGGAGGGAGCATGGAGCTGGGCTTTTAATCTATGGAGGAAGGTGAAGGATTTAGGGCATCATGCTGTGGCTAGCTTGGGTTACACGGATGATAGGAGGTTAGGAGAGTTGCAGGAATTGAGAGCTGAATTGGAGACTGAACTGGCTGCTTATAAAGAAGGCCGTGCAGTGGGTGCTGGAGTTGCATTTGTGGTGTTTAAGGATGTGTATACTGCTAATAAAGCAGTTCAGGACTTTCGACATGAGAAGAAGAGGCGGATAGGGAAGTTCTTTTCTCTCATGGAGTTGCGTTTACAGAGAAATCACTGGAAAGTTGAGCAGGCACCATTGGCAACAGACATTTATTGGAATCATTTGGGATCGTCCAAGGTCTCTCTGAAGTTGCGGAGAGTTCTTGTGAATACATGCTTACTGTTGATTCTTCTATTTTTTAGCTCTCCTCTTGCAATTATCAGTGCTGTGAAAAGTGCTGGACGGATCATAAATGCAGAAGCTATGGACAATGCCGATTTGTGGTTAGCTTGGTTTCAGAGCTCAAGCTGGCTAGGGAGCCTCATCTTTCAGTTTATGCCCAATGTCATTATTTTTATAAGCATGTATATCGTAATTCCATCAGCACTTTCCTATCTCTCCAAGTTTGAACGACATCTTACAGTTTCAGGAGAGCAGAGAGCTGCACTTCTTAAGATGGTTTGTTTCTTCCTGGTAAATCTTATTCTTCTTAAGGGTCTGGTTGAATCATCTTTAGAGAGTGCCCTGCTAAAAATGGGAAGGTGCTATTTGGATGGAGAAGATTGCAAGAGGATTGAGCAATACATGAGTGCATCATTTTTGTCAAGATCCTGCCTTTCCTCTCTGGCATTTTTGATTACAAGTACGTTTTTGGGGATATCATTTGATCTATTGGCTCCGATCCCTTGGATAAAAAGGAAGATTCAGAAGTTCCGGAAGAATGACATGCTGCAGCTGGTCCCAGAACAAAGTGAAGAGTATCCATTAGAAACTCAGGAGCCTGATACTCTACAAAGACCCTTGATTGTGGAGAACACATATTATGATTCTCCTAGGCTGAATGGAATGGATATGCCGGGACATGATCTTTCTGAATACCCCATCAACAGAACCTCTACAGCCCCCAAGCAGACATTCGATTTTGCGCAATATTATGCATTCAATTTGACAATATTTGCCCTCACATTTATCTATTCTTCATTTGCTCCACTCGTGGTTCCGGTTGGTGCAATATATTTCGGGTACAGGTACGTGGTTGATAAGTACAACTTTCTGTTTGTCTATCGGGTCCGTGGCTTTCCTGCTGGAAATGATGGGAAGTTGATGGATACAGTCTTGAGCATCATGCGTTTCTGTGTTGATTTGTACCTGCTTGCAATGCTCTTGTTCTTTTCGGTTCATGGAGACTCAACAAAGCTGCAAGCAATATTCACACTTGGGGTGTTAGTAATGTACAAACTGTTGCCTTCTCACAATGATAGATTTCATCCTGCTGTTTTGGAAGGCATACAAACTGTAGACAGTTTTGTGGATGGACCCATTGATTATGAGGTCTTTTCACAGCCTAAGTTTGATTGGGATACGTATTACACATAG

Protein Analysis

802

Amino Acids

90.35

Weight (kDa)

6.69

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 49 - 155 8.3e-12 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 186 - 385 5.4e-13 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 396 - 581 1.2e-11 Calcium-dependent channel, 7TM region, putative phosphate
RSN1_7TM PF02714 647 - 734 4.7e-12 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015059)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35870
fragaria_vesca FvH4_1g11170
malus_domestica MD02G1124200.v1.1 MD15G1238200.v1.1
prunus_persica Prupe.7G174200_v2.0.a1
pyrus_communis pycom02g09710 pycom05g15420 pycom15g21190
rosa_chinensis RchiOBHm_Chr2g0098461
rosa_laevigata RLG00000016800
rosa_multiflora Rmu_sc0010938.1_g000006
rosa_roxburghii Rroxscaffold_2G00143940
rosa_rugosa Rorug02G0073400
rosa_samantha Rh2AG120700 Rh2DG125700
rosa_wichuraiana Rw2G009460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 651, 954, 1391
Acc16I TGCGCA 1 cut(s) 1959
Acc36I ACCTGC 1 cut(s) 2190
AccB1I GGYRCC 2 cut(s) 236, 1123
AccBSI CCGCTC 1 cut(s) 141
AccI GTMKAC 2 cut(s) 1010, 2331
AccII CGCG 1 cut(s) 216
AccIII TCCGGA 1 cut(s) 1746
AclWI GGATC 4 cut(s) 1168, 1286, 1635, 1708
AcoI YGGCCR 2 cut(s) 211, 348
AcuI CTGAAG 1 cut(s) 1199
AcyI GRCGYC 2 cut(s) 237, 243
AfaI GTAC 8 cut(s) 59, 74, 1678, 2057, 2063, 2078, 2180, 2267
AfiI CCNNNNNNNGG 6 cut(s) 32, 38, 160, 538, 970, 1314
AflII CTTAAG 2 cut(s) 1484, 1523
AgsI TTSAA 5 cut(s) 331, 490, 1442, 1538, 1975
AjnI CCWGG 4 cut(s) 53, 532, 637, 1503
AjuI GAANNNNNNNTTGG 2 cut(s) 1931, 1963
Alw21I GWGCWC 2 cut(s) 357, 1337
Alw26I GTCTC 3 cut(s) 929, 1177, 2211
AlwI GGATC 4 cut(s) 1168, 1286, 1635, 1708
Aor13HI TCCGGA 1 cut(s) 1746
AoxI GGCC 4 cut(s) 211, 280, 348, 961
ApeKI GCWGC 9 cut(s) 179, 273, 276, 286, 947, 1475, 1762, 1765, 2230
AspA2I CCTAGG 1 cut(s) 1865
AspLEI GCGC 2 cut(s) 239, 1960
AspS9I GGNCC 5 cut(s) 164, 791, 1771, 2101, 2348
AsuC2I CCSGG 5 cut(s) 33, 162, 343, 754, 1890
AsuHPI GGTGA 1 cut(s) 856
AsuNHI GCTAGC 1 cut(s) 872
AvaII GGWCC 5 cut(s) 164, 791, 1771, 2101, 2348
AvrII CCTAGG 1 cut(s) 1865
BaeGI GKGCMC 1 cut(s) 1558
BanI GGYRCC 2 cut(s) 236, 1123
BanII GRGCYC 1 cut(s) 1337
BauI CACGAG 1 cut(s) 2024
Bbv12I GWGCWC 2 cut(s) 357, 1337
BbvCI CCTCAGC 1 cut(s) 169
BbvI GCAGC 9 cut(s) 191, 263, 273, 285, 934, 1462, 1749, 1777, 2217
BceAI ACGGC 8 cut(s) 10, 76, 179, 284, 293, 335, 363, 948
BcgI CGANNNNNNTGC 2 cut(s) 1384, 1418
BciT130I CCWGG 4 cut(s) 55, 534, 639, 1505
BciVI GTATCC 5 cut(s) 570, 645, 1803, 2134, 2386
BcnI CCSGG 5 cut(s) 33, 162, 343, 754, 1890
BcoDI GTCTC 3 cut(s) 929, 1177, 2211
BfaI CTAG 4 cut(s) 498, 873, 1346, 1866
BfmI CTRYAG 5 cut(s) 474, 588, 1763, 1929, 2328
BfoI RGCGCY 1 cut(s) 240
BfrI CTTAAG 2 cut(s) 1484, 1523
BfuAI ACCTGC 1 cut(s) 2190
BfuI GTATCC 5 cut(s) 570, 645, 1803, 2134, 2386
BglI GCCNNNNNGGC 2 cut(s) 234, 347
BlnI CCTAGG 1 cut(s) 1865
Bme1390I CCNGG 9 cut(s) 33, 55, 162, 343, 534, 639, 754, 1505, 1890
Bme18I GGWCC 5 cut(s) 164, 791, 1771, 2101, 2348
BmgT120I GGNCC 5 cut(s) 164, 791, 1771, 2101, 2348
BmrFI CCNGG 9 cut(s) 33, 55, 162, 343, 534, 639, 754, 1505, 1890
BmsI GCATC 3 cut(s) 868, 1634, 2163
BmtI GCTAGC 1 cut(s) 876
BpmI CTGGAG 1 cut(s) 999
Bpu10I CCTNAGC 1 cut(s) 169
BpuEI CTTGAG 2 cut(s) 1321, 2170
BpuMI CCSGG 5 cut(s) 33, 162, 343, 754, 1890
BsaAI YACGTR 2 cut(s) 2065, 2397
BsaHI GRCGYC 2 cut(s) 237, 243
BsaI GGTCTC 1 cut(s) 1177
BsaJI CCNNGG 6 cut(s) 31, 532, 1167, 1718, 1865, 2104
BsaWI WCCGGW 2 cut(s) 1746, 2032
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 6 cut(s) 32, 38, 160, 538, 970, 1314
Bse118I RCCGGY 1 cut(s) 793
Bse1I ACTGG 4 cut(s) 70, 214, 948, 1112
Bse3DI GCAATG 1 cut(s) 2196
BseAI TCCGGA 1 cut(s) 1746
BseBI CCWGG 4 cut(s) 55, 534, 639, 1505
BseDI CCNNGG 6 cut(s) 31, 532, 1167, 1718, 1865, 2104
BseGI GGATG 7 cut(s) 66, 514, 895, 1009, 1591, 2299, 2350
BseLI CCNNNNNNNGG 6 cut(s) 32, 38, 160, 538, 970, 1314
BseMI GCAATG 1 cut(s) 2196
BseMII CTCAG 2 cut(s) 183, 1820
BseNI ACTGG 4 cut(s) 70, 214, 948, 1112
BseRI GAGGAG 4 cut(s) 9, 68, 179, 1230
BseSI GKGCMC 1 cut(s) 1558
BseX3I CGGCCG 1 cut(s) 348
BseXI GCAGC 9 cut(s) 191, 263, 273, 285, 934, 1462, 1749, 1777, 2217
BseYI CCCAGC 1 cut(s) 819
BsgI GTGCAG 3 cut(s) 590, 987, 1461
Bsh1236I CGCG 1 cut(s) 216
Bsh1285I CGRYCG 1 cut(s) 351
BshFI GGCC 4 cut(s) 213, 282, 350, 963
BshNI GGYRCC 2 cut(s) 236, 1123
BsiEI CGRYCG 1 cut(s) 351
BsiHKAI GWGCWC 2 cut(s) 357, 1337
BsiSI CCGG 8 cut(s) 33, 161, 342, 754, 794, 1747, 1889, 2033
BslFI GGGAC 6 cut(s) 13, 177, 358, 616, 1757, 1905
BslI CCNNNNNNNGG 6 cut(s) 32, 38, 160, 538, 970, 1314
BsmAI GTCTC 3 cut(s) 929, 1177, 2211
BsmFI GGGAC 6 cut(s) 13, 177, 358, 616, 1757, 1905
BsmI GAATGC 1 cut(s) 1970
BsnI GGCC 4 cut(s) 213, 282, 350, 963
Bso31I GGTCTC 1 cut(s) 1177
Bsp1286I GDGCHC 3 cut(s) 357, 1337, 1558
Bsp13I TCCGGA 1 cut(s) 1746
Bsp1407I TGTACA 1 cut(s) 2265
Bsp143I GATC 8 cut(s) 361, 492, 1160, 1278, 1640, 1699, 1713, 1897
BspANI GGCC 4 cut(s) 213, 282, 350, 963
BspCNI CTCAG 2 cut(s) 182, 1819
BspEI TCCGGA 1 cut(s) 1746
BspFNI CGCG 1 cut(s) 216
BspMAI CTGCAG 1 cut(s) 1767
BspMI ACCTGC 1 cut(s) 2190
BspOI GCTAGC 1 cut(s) 876
BspPI GGATC 4 cut(s) 1168, 1286, 1635, 1708
BspT107I GGYRCC 2 cut(s) 236, 1123
BspTI CTTAAG 2 cut(s) 1484, 1523
BspTNI GGTCTC 1 cut(s) 1177
BsrBI CCGCTC 1 cut(s) 141
BsrDI GCAATG 1 cut(s) 2196
BsrFI RCCGGY 1 cut(s) 793
BsrGI TGTACA 1 cut(s) 2265
BsrI ACTGG 4 cut(s) 70, 214, 948, 1112
BssAI RCCGGY 1 cut(s) 793
BssECI CCNNGG 6 cut(s) 31, 532, 1167, 1718, 1865, 2104
BssMI GATC 8 cut(s) 361, 492, 1160, 1278, 1640, 1699, 1713, 1897
BssNAI GTATAC 1 cut(s) 1011
BssNI GRCGYC 2 cut(s) 237, 243
BssSI CACGAG 1 cut(s) 2024
BssT1I CCWWGG 3 cut(s) 1167, 1718, 1865
Bst1107I GTATAC 1 cut(s) 1011
Bst2BI CACGAG 1 cut(s) 2024
Bst2UI CCWGG 4 cut(s) 55, 534, 639, 1505
Bst4CI ACNGT 7 cut(s) 607, 1215, 1456, 2146, 2274, 2329, 2336
Bst6I CTCTTC 4 cut(s) 119, 131, 1046, 1782
BstACI GRCGYC 2 cut(s) 237, 243
BstAFI CTTAAG 2 cut(s) 1484, 1523
BstAPI GCANNNNNTGC 1 cut(s) 974
BstAUI TGTACA 1 cut(s) 2265
BstBAI YACGTR 2 cut(s) 2065, 2397
BstC8I GCNNGC 9 cut(s) 158, 204, 226, 284, 874, 1122, 1343, 2187, 2235
BstDEI CTNAG 3 cut(s) 169, 1806, 2379
BstDSI CCRYGG 1 cut(s) 2104
BstF5I GGATG 7 cut(s) 66, 514, 895, 1009, 1591, 2299, 2350
BstFNI CGCG 1 cut(s) 216
BstH2I RGCGCY 1 cut(s) 240
BstHHI GCGC 2 cut(s) 239, 1960
BstKTI GATC 8 cut(s) 364, 495, 1163, 1281, 1643, 1702, 1716, 1900
BstMAI GTCTC 3 cut(s) 929, 1177, 2211
BstMBI GATC 8 cut(s) 361, 492, 1160, 1278, 1640, 1699, 1713, 1897
BstMCI CGRYCG 1 cut(s) 351
BstNI CCWGG 4 cut(s) 55, 534, 639, 1505
BstNSI RCATGY 4 cut(s) 160, 1209, 1399, 1762
BstSCI CCNGG 9 cut(s) 31, 53, 160, 341, 532, 637, 752, 1503, 1888
BstSFI CTRYAG 5 cut(s) 474, 588, 1763, 1929, 2328
BstSLI GKGCMC 1 cut(s) 1558
BstSNI TACGTA 1 cut(s) 2397
BstUI CGCG 1 cut(s) 216
BstV1I GCAGC 9 cut(s) 191, 263, 273, 285, 934, 1462, 1749, 1777, 2217
BstX2I RGATCY 1 cut(s) 1640
BstYI RGATCY 1 cut(s) 1640
BstZ17I GTATAC 1 cut(s) 1011
BstZI CGGCCG 1 cut(s) 348
BsuI GTATCC 5 cut(s) 570, 645, 1803, 2134, 2386
BsuRI GGCC 4 cut(s) 213, 282, 350, 963
BtgI CCRYGG 1 cut(s) 2104
BtgZI GCGATG 1 cut(s) 51
BtsCI GGATG 7 cut(s) 66, 514, 895, 1009, 1591, 2299, 2350
BtsI GCAGTG 2 cut(s) 229, 975
BtsIMutI CAGTG 5 cut(s) 207, 229, 975, 1105, 1261
BveI ACCTGC 1 cut(s) 2190
Cac8I GCNNGC 9 cut(s) 158, 204, 226, 284, 874, 1122, 1343, 2187, 2235
CfoI GCGC 2 cut(s) 239, 1960
Cfr10I RCCGGY 1 cut(s) 793
Cfr13I GGNCC 5 cut(s) 164, 791, 1771, 2101, 2348
CseI GACGC 2 cut(s) 251, 684
Csp6I GTAC 8 cut(s) 58, 73, 1677, 2056, 2062, 2077, 2179, 2266
CviQI GTAC 8 cut(s) 58, 73, 1677, 2056, 2062, 2077, 2179, 2266
DdeI CTNAG 3 cut(s) 169, 1806, 2379
DinI GGCGCC 1 cut(s) 238
DpnI GATC 8 cut(s) 363, 494, 1162, 1280, 1642, 1701, 1715, 1899
DpnII GATC 8 cut(s) 361, 492, 1160, 1278, 1640, 1699, 1713, 1897
EaeI YGGCCR 2 cut(s) 211, 348
EagI CGGCCG 1 cut(s) 348
Eam1104I CTCTTC 4 cut(s) 119, 131, 1046, 1782
EarI CTCTTC 4 cut(s) 119, 131, 1046, 1782
EciI GGCGGA 2 cut(s) 12, 1072
Ecl136II GAGCTC 1 cut(s) 1335
EclXI CGGCCG 1 cut(s) 348
Eco105I TACGTA 1 cut(s) 2397
Eco130I CCWWGG 3 cut(s) 1167, 1718, 1865
Eco24I GRGCYC 1 cut(s) 1337
Eco31I GGTCTC 1 cut(s) 1177
Eco32I GATATC 1 cut(s) 1692
Eco47I GGWCC 5 cut(s) 164, 791, 1771, 2101, 2348
Eco52I CGGCCG 1 cut(s) 348
Eco53kI GAGCTC 1 cut(s) 1335
Eco57I CTGAAG 1 cut(s) 1199
EcoICRI GAGCTC 1 cut(s) 1335
EcoO109I RGGNCCY 1 cut(s) 164
EcoRII CCWGG 4 cut(s) 53, 532, 637, 1503
EcoRV GATATC 1 cut(s) 1692
EcoT14I CCWWGG 3 cut(s) 1167, 1718, 1865
EcoT22I ATGCAT 1 cut(s) 1972
EcoT38I GRGCYC 1 cut(s) 1337
EgeI GGCGCC 1 cut(s) 238
EheI GGCGCC 1 cut(s) 238
ErhI CCWWGG 3 cut(s) 1167, 1718, 1865
FalI AAGNNNNNCTT 2 cut(s) 2066, 2098
FaqI GGGAC 6 cut(s) 13, 177, 358, 616, 1757, 1905
FauI CCCGC 1 cut(s) 233
FblI GTMKAC 2 cut(s) 1010, 2331
FokI GGATG 7 cut(s) 53, 521, 902, 1016, 1598, 2286, 2357
FriOI GRGCYC 1 cut(s) 1337
FspBI CTAG 4 cut(s) 498, 873, 1346, 1866
FspI TGCGCA 1 cut(s) 1959
GlaI GCGC 2 cut(s) 238, 1959
GsaI CCCAGC 1 cut(s) 823
GsuI CTGGAG 1 cut(s) 999
HaeII RGCGCY 1 cut(s) 240
HaeIII GGCC 4 cut(s) 213, 282, 350, 963
HapII CCGG 8 cut(s) 33, 161, 342, 754, 794, 1747, 1889, 2033
HgaI GACGC 2 cut(s) 251, 684
HhaI GCGC 2 cut(s) 239, 1960
Hin1I GRCGYC 2 cut(s) 237, 243
Hin6I GCGC 2 cut(s) 237, 1958
HinP1I GCGC 2 cut(s) 237, 1958
HpaII CCGG 8 cut(s) 33, 161, 342, 754, 794, 1747, 1889, 2033
HphI GGTGA 1 cut(s) 856
Hpy166II GTNNAC 3 cut(s) 1011, 1094, 2332
Hpy188I TCNGA 9 cut(s) 145, 541, 700, 805, 1179, 1332, 1713, 1740, 1906
Hpy188III TCNNGA 7 cut(s) 394, 1031, 1461, 1638, 1747, 1808, 2149
Hpy8I GTNNAC 3 cut(s) 1011, 1094, 2332
Hpy99I CGWCG 2 cut(s) 245, 700
HpyAV CCTTC 8 cut(s) 112, 260, 835, 841, 953, 1566, 2289, 2309
HpyCH4III ACNGT 7 cut(s) 607, 1215, 1456, 2146, 2274, 2329, 2336
HpyCH4IV ACGT 3 cut(s) 1679, 2064, 2396
HpyF3I CTNAG 3 cut(s) 169, 1806, 2379
HpySE526I ACGT 3 cut(s) 1679, 2064, 2396
Hsp92I GRCGYC 2 cut(s) 237, 243
HspAI GCGC 2 cut(s) 237, 1958
KasI GGCGCC 1 cut(s) 236
KflI GGGWCCC 1 cut(s) 164
Kpn2I TCCGGA 1 cut(s) 1746
Kzo9I GATC 8 cut(s) 361, 492, 1160, 1278, 1640, 1699, 1713, 1897
Lsp1109I GCAGC 9 cut(s) 191, 263, 273, 285, 934, 1462, 1749, 1777, 2217
LweI GCATC 3 cut(s) 868, 1634, 2163
MaeI CTAG 4 cut(s) 498, 873, 1346, 1866
MaeII ACGT 3 cut(s) 1679, 2064, 2396
MaeIII GTNAC 2 cut(s) 527, 881
MalI GATC 8 cut(s) 363, 494, 1162, 1280, 1642, 1701, 1715, 1899
MbiI CCGCTC 1 cut(s) 141
MboI GATC 8 cut(s) 361, 492, 1160, 1278, 1640, 1699, 1713, 1897
MflI RGATCY 1 cut(s) 1640
MhlI GDGCHC 3 cut(s) 357, 1337, 1558
Mly113I GGCGCC 1 cut(s) 237
MlyI GAGTC 3 cut(s) 488, 743, 2213
MmeI TCCRAC 3 cut(s) 168, 678, 783
Mph1103I ATGCAT 1 cut(s) 1972
MroI TCCGGA 1 cut(s) 1746
MseI TTAA 5 cut(s) 708, 828, 999, 1485, 1524
MspA1I CMGCKG 2 cut(s) 276, 1768
MspCI CTTAAG 2 cut(s) 1484, 1523
MspI CCGG 8 cut(s) 33, 161, 342, 754, 794, 1747, 1889, 2033
MspR9I CCNGG 9 cut(s) 33, 55, 162, 343, 534, 639, 754, 1505, 1890
Mva1269I GAATGC 1 cut(s) 1970
MvaI CCWGG 4 cut(s) 55, 534, 639, 1505
MvnI CGCG 1 cut(s) 216
NarI GGCGCC 1 cut(s) 237
NciI CCSGG 5 cut(s) 33, 162, 343, 754, 1890
NdeII GATC 8 cut(s) 361, 492, 1160, 1278, 1640, 1699, 1713, 1897
NheI GCTAGC 1 cut(s) 872
NmeAIII GCCGAG 1 cut(s) 336
NmuCI GTSAC 1 cut(s) 527
NsbI TGCGCA 1 cut(s) 1959
NsiI ATGCAT 1 cut(s) 1972
NspI RCATGY 4 cut(s) 160, 1209, 1399, 1762
PaeI GCATGC 1 cut(s) 160
PcsI WCGNNNNNNNCGW 1 cut(s) 377
PctI GAATGC 1 cut(s) 1970
PfeI GAWTC 7 cut(s) 331, 542, 1150, 1219, 1538, 1735, 1859
PleI GAGTC 3 cut(s) 487, 743, 2213
PluTI GGCGCC 1 cut(s) 240
PpsI GAGTC 3 cut(s) 487, 743, 2213
Ppu21I YACGTR 2 cut(s) 2065, 2397
PpuMI RGGWCCY 1 cut(s) 164
PsiI TTATAA 3 cut(s) 651, 954, 1391
Psp124BI GAGCTC 1 cut(s) 1337
Psp5II RGGWCCY 1 cut(s) 164
Psp6I CCWGG 4 cut(s) 53, 532, 637, 1503
PspFI CCCAGC 1 cut(s) 819
PspGI CCWGG 4 cut(s) 53, 532, 637, 1503
PspPI GGNCC 5 cut(s) 164, 791, 1771, 2101, 2348
PspPPI RGGWCCY 1 cut(s) 164
PstI CTGCAG 1 cut(s) 1767
PsuI RGATCY 1 cut(s) 1640
PvuII CAGCTG 2 cut(s) 276, 1768
RsaI GTAC 8 cut(s) 59, 74, 1678, 2057, 2063, 2078, 2180, 2267
RsaNI GTAC 8 cut(s) 58, 73, 1677, 2056, 2062, 2077, 2179, 2266
SacI GAGCTC 1 cut(s) 1337
SaqAI TTAA 5 cut(s) 708, 828, 999, 1485, 1524
Sau3AI GATC 8 cut(s) 361, 492, 1160, 1278, 1640, 1699, 1713, 1897
Sau96I GGNCC 5 cut(s) 164, 791, 1771, 2101, 2348
SchI GAGTC 3 cut(s) 488, 743, 2213
ScrFI CCNGG 9 cut(s) 33, 55, 162, 343, 534, 639, 754, 1505, 1890
SduI GDGCHC 3 cut(s) 357, 1337, 1558
SfaNI GCATC 3 cut(s) 868, 1634, 2163
SfcI CTRYAG 5 cut(s) 474, 588, 1763, 1929, 2328
SfoI GGCGCC 1 cut(s) 238
SinI GGWCC 5 cut(s) 164, 791, 1771, 2101, 2348
SmlI CTYRAG 4 cut(s) 1336, 1484, 1523, 2149
SmoI CTYRAG 4 cut(s) 1336, 1484, 1523, 2149
SnaBI TACGTA 1 cut(s) 2397
SphI GCATGC 1 cut(s) 160
SspDI GGCGCC 1 cut(s) 236
SspI AATATT 3 cut(s) 1964, 1986, 2241
SspMI CTAG 4 cut(s) 498, 873, 1346, 1866
SstI GAGCTC 1 cut(s) 1337
StyD4I CCNGG 9 cut(s) 31, 53, 160, 341, 532, 637, 752, 1503, 1888
StyI CCWWGG 3 cut(s) 1167, 1718, 1865
TaaI ACNGT 7 cut(s) 607, 1215, 1456, 2146, 2274, 2329, 2336
TaiI ACGT 3 cut(s) 1682, 2067, 2399
TaqI TCGA 4 cut(s) 264, 393, 1039, 1950
TatI WGTACW 2 cut(s) 2076, 2265
TauI GCSGC 7 cut(s) 53, 156, 216, 237, 248, 273, 282
TfiI GAWTC 7 cut(s) 331, 542, 1150, 1219, 1538, 1735, 1859
Tru1I TTAA 5 cut(s) 708, 828, 999, 1485, 1524
Tru9I TTAA 5 cut(s) 708, 828, 999, 1485, 1524
TscAI CASTG 5 cut(s) 214, 236, 975, 1112, 1261
TseFI GTSAC 1 cut(s) 527
TseI GCWGC 9 cut(s) 179, 273, 276, 286, 947, 1475, 1762, 1765, 2230
Tsp45I GTSAC 1 cut(s) 527
TspDTI ATGAA 6 cut(s) 17, 106, 735, 2001, 2201, 2288
TspGWI ACGGA 4 cut(s) 315, 902, 1291, 2093
TspRI CASTG 5 cut(s) 214, 236, 975, 1112, 1261
Vha464I CTTAAG 2 cut(s) 1484, 1523
VpaK11BI GGWCC 5 cut(s) 164, 791, 1771, 2101, 2348
XceI RCATGY 4 cut(s) 160, 1209, 1399, 1762
XmaJI CCTAGG 1 cut(s) 1865
XmiI GTMKAC 2 cut(s) 1010, 2331
XspI CTAG 4 cut(s) 498, 873, 1346, 1866
Zsp2I ATGCAT 1 cut(s) 1972
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.