Rroxscaffold_2G00143940

Calcium-dependent channel, 7TM region, putative phosphate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0015059
Physical Location & Seq
Reverse (-)
81846378 .. 81850379
4002 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00143940.1

Sequence Viewer

Length: 2505 bp
ATGTTAGCATCAATCCCCATCCCAGCAGCAGGACCGAGCGACGTAGGCGAGGCCTATGCTCGGCCGCCTCGGAACAAGGGCGATCTCTCGCTCCTCCATGAACGACACTCTATCTCCTCCGCCGTCCCCGGCGATGGCGACTACGCCGCCTGGTACGGCAACATCCAGTACCTCCTCAACATCTCCGCCATCGGCTCCTTCTTCTGCCTCTTCATCTTCCTCTTCGTCAAGCTCCGCTCCGACCACCGCCGCATGCCGGGACCCTCCGCCCTCGCCGCCAAGCTCCTCGCCGTCTGGCACGCGACTGGCCGCGAAATCGCCCGCCACTGCGGCGCCGACGCCGCCCAATTTCTCCTAATCGAAGGCGGCAGCTGCGGCCTGCTGCTCTCCCTCGCCGTCCTCGCCGTGCTGGTCATGCTTCCGTTGAATCTCTACGCCGGGACGGCCGTGCTCGGCGATCAATTCTCGCAGACGACGATAAACCATATCGAGAAAGGTTCAGCTTTACTTTGGGTGCATTTTGTTTTTCTGGTTGTGGTTGTTGTTTTCGTGCATTTTGGTATTTCTGCTATAGAGTCAAGGTTGAAGATCACTAGGATTAGGGATGGGAATGGGAATATGAGTGACCCAGGTTCAGATTCCACAGCTCTTTTCACTATAATGGTGCAGGGGATACCGAAAATTATAGGGAGTGATGGGACTGTGTTGCATGAGTATTTTCAGCATAAGTATCCTGGCAAGGTTTATAAGGTTGTTTTGCCAATGGATTTGTGTGCATTGGAAGAATTAGCATCGGAGTTGGTTAAGGTTAGGCATGAAATTGCTTGGTTGGTTGCGAAAATTGACTCCCGGCTCTTGCCTGATGAGAGTATAGAAAATGGATATGGACCGGCTTTGTCGGAGGGAGCATGGGGCTGGGCTTTTAATCTATGGAGGAAGGTGAAGGATTTAGGGCATCATGCTGTGGCTAGCTTGGGTTACACGGATGATAGGAGGTTAGGAGAGTTGCAGGAATTGAGAGCTGAATTGGAGACTGAACTGGCTGCTTATAAAGAAGGCCGTGCAGTGGGTGCTGGAGTTGCGTTTGTGGTGTTTAAGGATGTGTACACTGCTAATAAAGCGGTTCAGGACTTTCGACATGAGAAGAAGAGGCGGATAGGGAAGTTCTTTTCTCTCATGGAGTTGCGTTTACAGAGAAATCACTGGAAAGTTGAACAGGCACCATTGGCAACAGACATTTATTGGAATCATTTGGGATCGTCCAAGGTCTCACTGAAGTTGCGGAGAGTTCTTGTGAATACATGCTTACTGTTGATTCTTCTATTTTTTAGCTCTCCTCTTGCAATTATCAGTGCTGTGAAAAGTGCTGGACGGATCATAAATGCAGAAGCTATGGACAATGCCGATTTGTGGTTAGCTTGGTTTCAGAGCTCAAGCTGGCTAGGGAGCCTCATCTTTCAGTTTATGCCCAATGTCATTATTTTTATAAGCATGTATATCGTAATTCCATCAGCACTTTCCTATCTCTCCAAGTTTGAACGACATCTTACAGTTTCAGGAGAGCAGAGAGCCGCACTTCTTAAGATGGTTTGTTTCTTCCTGGTAAATCTTATTCTTCTTAAGGGTCTGGTTGAATCATCTTTAGAGAGTGCCCTGCTAAAAATGGGAAGGTGCTATTTGGATGGAGAAGATTGCAAGAGGATTGAGCAGTACATGAGTGCATCATTTTTGTCAAGATCCTGCCTTTCGTCTCTGGCATTTTTGATTACAAGTACGTTTTTGGGGATATCATTTGATCTATTGGCTCCGATCCCTTGGATAAAAAGGAAGATTCAGAAGTTCCGGAAGAATGACATGCTGCAGCTGGTCCCAGAACAAAGTGAAGAGTACCCATTAGAAACTCAGGAGCCTGATACTCTACAAAGACCCTTCATTGTGGAGAACACATATTATGATTCTCCTAGGCTGAATGGAATGGATATGCCGGGACATGATCTTTCTGAATACCCCATCAACAGAACCTCTACAGCCCCCAAGCAGACATTCGATTTTGCGCAATATTATGCATTCAATTTGACAATATTTGCCCTCACATTTATCTATTCTTCATTTGCTCCACTCGTGGTTCCGGTTGGTGCAATATATTTCGGGTACAGGTACGTGGTTGATAAGTACAACTTTCTGTTTGTCTATCGGGTCCGCGGCTTTCCTGCTGGAAATGATGGGAAGTTGATGGATACAGTCTTGAGCATCATGCGTTTCTGTGTTGATTTGTACCTGCTTGCAATGCTCTTGTTCTTTTCGGTTCATGGAGACTCAACAAAGCTGCAAGCAATATTCACGCTTGGGGTGTTAGTAATGTACAAACTGTTGCCTTCTCACAATGATACATTTCATCCTGCTGTTTTGGAAGGCATACAAACTGTAGACAGTTTTCTGGATGGACCCATTGATTATGAGGTCTTTTCACAGCCTAAGTTTGATTGGGATACGTATTATACATAG

Protein Analysis

834

Amino Acids

93.65

Weight (kDa)

6.73

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 81 - 187 7.9e-12 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 218 - 417 1.7e-12 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 428 - 613 1.3e-11 Calcium-dependent channel, 7TM region, putative phosphate
RSN1_7TM PF02714 679 - 766 5e-12 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015059)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35870
fragaria_vesca FvH4_1g11170
malus_domestica MD02G1124200.v1.1 MD15G1238200.v1.1
prunus_persica Prupe.7G174200_v2.0.a1
pyrus_communis pycom02g09710 pycom05g15420 pycom15g21190
rosa_chinensis RchiOBHm_Chr2g0098461
rosa_laevigata RLG00000016800
rosa_multiflora Rmu_sc0010938.1_g000006
rosa_roxburghii Rroxscaffold_2G00143940
rosa_rugosa Rorug02G0073400
rosa_samantha Rh2AG120700 Rh2DG125700
rosa_wichuraiana Rw2G009460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 747, 1050, 1487
Acc16I TGCGCA 1 cut(s) 2055
Acc36I ACCTGC 1 cut(s) 2286
AccB1I GGYRCC 2 cut(s) 332, 1219
AccBSI CCGCTC 1 cut(s) 237
AccI GTMKAC 1 cut(s) 2427
AccII CGCG 3 cut(s) 302, 312, 2202
AccIII TCCGGA 1 cut(s) 1842
AclWI GGATC 4 cut(s) 1264, 1382, 1731, 1804
AcoI YGGCCR 3 cut(s) 62, 307, 444
AcuI CTGAAG 1 cut(s) 1295
AcyI GRCGYC 2 cut(s) 333, 339
AfiI CCNNNNNNNGG 6 cut(s) 29, 60, 134, 256, 1066, 1410
AflII CTTAAG 2 cut(s) 1580, 1619
AgsI TTSAA 6 cut(s) 427, 586, 1214, 1538, 1634, 2071
AjnI CCWGG 4 cut(s) 149, 628, 733, 1599
AjuI GAANNNNNNNTTGG 2 cut(s) 2027, 2059
Alw21I GWGCWC 2 cut(s) 453, 1433
Alw26I GTCTC 4 cut(s) 1025, 1273, 1755, 2307
AlwI GGATC 4 cut(s) 1264, 1382, 1731, 1804
Aor13HI TCCGGA 1 cut(s) 1842
AoxI GGCC 6 cut(s) 51, 62, 307, 376, 444, 1057
ApeKI GCWGC 8 cut(s) 26, 369, 372, 382, 1043, 1858, 1861, 2326
AspA2I CCTAGG 1 cut(s) 1961
AspLEI GCGC 2 cut(s) 335, 2056
AspS9I GGNCC 6 cut(s) 32, 260, 887, 1867, 2197, 2444
AsuC2I CCSGG 5 cut(s) 129, 258, 439, 850, 1986
AsuHPI GGTGA 1 cut(s) 952
AsuNHI GCTAGC 1 cut(s) 968
AvaII GGWCC 6 cut(s) 32, 260, 887, 1867, 2197, 2444
AvrII CCTAGG 1 cut(s) 1961
BaeGI GKGCMC 1 cut(s) 1654
BanI GGYRCC 2 cut(s) 332, 1219
BanII GRGCYC 1 cut(s) 1433
BauI CACGAG 1 cut(s) 2120
Bbv12I GWGCWC 2 cut(s) 453, 1433
BbvI GCAGC 8 cut(s) 38, 359, 369, 381, 1030, 1845, 1873, 2313
BceAI ACGGC 8 cut(s) 107, 172, 275, 380, 389, 431, 459, 1044
BcgI CGANNNNNNTGC 4 cut(s) 38, 72, 1480, 1514
BciT130I CCWGG 4 cut(s) 151, 630, 735, 1601
BciVI GTATCC 4 cut(s) 666, 741, 2230, 2482
BcnI CCSGG 5 cut(s) 129, 258, 439, 850, 1986
BcoDI GTCTC 4 cut(s) 1025, 1273, 1755, 2307
BfaI CTAG 4 cut(s) 594, 969, 1442, 1962
BfmI CTRYAG 4 cut(s) 570, 1859, 2025, 2424
BfoI RGCGCY 1 cut(s) 336
BfrI CTTAAG 2 cut(s) 1580, 1619
BfuAI ACCTGC 1 cut(s) 2286
BfuI GTATCC 4 cut(s) 666, 741, 2230, 2482
BglI GCCNNNNNGGC 2 cut(s) 330, 443
BlnI CCTAGG 1 cut(s) 1961
Bme1390I CCNGG 9 cut(s) 129, 151, 258, 439, 630, 735, 850, 1601, 1986
Bme18I GGWCC 6 cut(s) 32, 260, 887, 1867, 2197, 2444
BmgT120I GGNCC 6 cut(s) 32, 260, 887, 1867, 2197, 2444
BmrFI CCNGG 9 cut(s) 129, 151, 258, 439, 630, 735, 850, 1601, 1986
BmsI GCATC 5 cut(s) 17, 800, 964, 1730, 2259
BmtI GCTAGC 1 cut(s) 972
BpmI CTGGAG 1 cut(s) 1095
BpuEI CTTGAG 2 cut(s) 1417, 2266
BpuMI CCSGG 5 cut(s) 129, 258, 439, 850, 1986
BsaAI YACGTR 2 cut(s) 2161, 2493
BsaHI GRCGYC 2 cut(s) 333, 339
BsaI GGTCTC 1 cut(s) 1273
BsaJI CCNNGG 7 cut(s) 68, 127, 628, 1263, 1814, 1961, 2200
BsaWI WCCGGW 2 cut(s) 1842, 2128
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 6 cut(s) 29, 60, 134, 256, 1066, 1410
Bse118I RCCGGY 1 cut(s) 889
Bse1I ACTGG 4 cut(s) 166, 310, 1044, 1208
Bse3DI GCAATG 1 cut(s) 2292
BseAI TCCGGA 1 cut(s) 1842
BseBI CCWGG 4 cut(s) 151, 630, 735, 1601
BseDI CCNNGG 7 cut(s) 68, 127, 628, 1263, 1814, 1961, 2200
BseGI GGATG 8 cut(s) 18, 162, 610, 991, 1105, 1687, 2395, 2446
BseLI CCNNNNNNNGG 6 cut(s) 29, 60, 134, 256, 1066, 1410
BseMI GCAATG 1 cut(s) 2292
BseMII CTCAG 1 cut(s) 1916
BseNI ACTGG 4 cut(s) 166, 310, 1044, 1208
BseRI GAGGAG 5 cut(s) 83, 106, 164, 275, 1326
BseSI GKGCMC 1 cut(s) 1654
BseX3I CGGCCG 2 cut(s) 62, 444
BseXI GCAGC 8 cut(s) 38, 359, 369, 381, 1030, 1845, 1873, 2313
BseYI CCCAGC 2 cut(s) 22, 915
BsgI GTGCAG 2 cut(s) 686, 1083
Bsh1236I CGCG 3 cut(s) 302, 312, 2202
Bsh1285I CGRYCG 2 cut(s) 65, 447
BshFI GGCC 6 cut(s) 53, 64, 309, 378, 446, 1059
BshNI GGYRCC 2 cut(s) 332, 1219
BsiEI CGRYCG 2 cut(s) 65, 447
BsiHKAI GWGCWC 2 cut(s) 453, 1433
BsiSI CCGG 8 cut(s) 129, 257, 438, 850, 890, 1843, 1985, 2129
BslFI GGGAC 6 cut(s) 110, 273, 454, 712, 1853, 2001
BslI CCNNNNNNNGG 6 cut(s) 29, 60, 134, 256, 1066, 1410
BsmAI GTCTC 4 cut(s) 1025, 1273, 1755, 2307
BsmBI CGTCTC 1 cut(s) 1755
BsmFI GGGAC 6 cut(s) 110, 273, 454, 712, 1853, 2001
BsmI GAATGC 1 cut(s) 2066
BsnI GGCC 6 cut(s) 53, 64, 309, 378, 446, 1059
Bso31I GGTCTC 1 cut(s) 1273
Bsp1286I GDGCHC 3 cut(s) 453, 1433, 1654
Bsp13I TCCGGA 1 cut(s) 1842
Bsp1407I TGTACA 2 cut(s) 1104, 2361
Bsp143I GATC 9 cut(s) 82, 457, 588, 1256, 1374, 1736, 1795, 1809, 1993
BspANI GGCC 6 cut(s) 53, 64, 309, 378, 446, 1059
BspCNI CTCAG 1 cut(s) 1915
BspEI TCCGGA 1 cut(s) 1842
BspFNI CGCG 3 cut(s) 302, 312, 2202
BspMAI CTGCAG 1 cut(s) 1863
BspMI ACCTGC 1 cut(s) 2286
BspOI GCTAGC 1 cut(s) 972
BspPI GGATC 4 cut(s) 1264, 1382, 1731, 1804
BspT107I GGYRCC 2 cut(s) 332, 1219
BspTI CTTAAG 2 cut(s) 1580, 1619
BspTNI GGTCTC 1 cut(s) 1273
BsrBI CCGCTC 1 cut(s) 237
BsrDI GCAATG 1 cut(s) 2292
BsrFI RCCGGY 1 cut(s) 889
BsrGI TGTACA 2 cut(s) 1104, 2361
BsrI ACTGG 4 cut(s) 166, 310, 1044, 1208
BssAI RCCGGY 1 cut(s) 889
BssECI CCNNGG 7 cut(s) 68, 127, 628, 1263, 1814, 1961, 2200
BssMI GATC 9 cut(s) 82, 457, 588, 1256, 1374, 1736, 1795, 1809, 1993
BssNI GRCGYC 2 cut(s) 333, 339
BssSI CACGAG 1 cut(s) 2120
BssT1I CCWWGG 3 cut(s) 1263, 1814, 1961
Bst2BI CACGAG 1 cut(s) 2120
Bst2UI CCWGG 4 cut(s) 151, 630, 735, 1601
Bst4CI ACNGT 7 cut(s) 703, 1311, 1552, 2242, 2370, 2425, 2432
Bst6I CTCTTC 4 cut(s) 215, 227, 1142, 1878
BstACI GRCGYC 2 cut(s) 333, 339
BstAFI CTTAAG 2 cut(s) 1580, 1619
BstAPI GCANNNNNTGC 1 cut(s) 1070
BstAUI TGTACA 2 cut(s) 1104, 2361
BstBAI YACGTR 2 cut(s) 2161, 2493
BstC8I GCNNGC 8 cut(s) 254, 300, 322, 380, 970, 1439, 2283, 2331
BstDEI CTNAG 2 cut(s) 1902, 2475
BstDSI CCRYGG 1 cut(s) 2200
BstF5I GGATG 8 cut(s) 18, 162, 610, 991, 1105, 1687, 2395, 2446
BstFNI CGCG 3 cut(s) 302, 312, 2202
BstH2I RGCGCY 1 cut(s) 336
BstHHI GCGC 2 cut(s) 335, 2056
BstKTI GATC 9 cut(s) 85, 460, 591, 1259, 1377, 1739, 1798, 1812, 1996
BstMAI GTCTC 4 cut(s) 1025, 1273, 1755, 2307
BstMBI GATC 9 cut(s) 82, 457, 588, 1256, 1374, 1736, 1795, 1809, 1993
BstMCI CGRYCG 2 cut(s) 65, 447
BstNI CCWGG 4 cut(s) 151, 630, 735, 1601
BstNSI RCATGY 4 cut(s) 256, 1305, 1495, 1858
BstSCI CCNGG 9 cut(s) 127, 149, 256, 437, 628, 733, 848, 1599, 1984
BstSFI CTRYAG 4 cut(s) 570, 1859, 2025, 2424
BstSLI GKGCMC 1 cut(s) 1654
BstSNI TACGTA 1 cut(s) 2493
BstUI CGCG 3 cut(s) 302, 312, 2202
BstV1I GCAGC 8 cut(s) 38, 359, 369, 381, 1030, 1845, 1873, 2313
BstX2I RGATCY 1 cut(s) 1736
BstYI RGATCY 1 cut(s) 1736
BstZI CGGCCG 2 cut(s) 62, 444
BsuI GTATCC 4 cut(s) 666, 741, 2230, 2482
BsuRI GGCC 6 cut(s) 53, 64, 309, 378, 446, 1059
BtgI CCRYGG 1 cut(s) 2200
BtgZI GCGATG 1 cut(s) 147
BtsCI GGATG 8 cut(s) 18, 162, 610, 991, 1105, 1687, 2395, 2446
BtsI GCAGTG 3 cut(s) 325, 1071, 1107
BtsIMutI CAGTG 6 cut(s) 325, 1071, 1107, 1201, 1271, 1357
BveI ACCTGC 1 cut(s) 2286
Cac8I GCNNGC 8 cut(s) 254, 300, 322, 380, 970, 1439, 2283, 2331
CfoI GCGC 2 cut(s) 335, 2056
Cfr10I RCCGGY 1 cut(s) 889
Cfr13I GGNCC 6 cut(s) 32, 260, 887, 1867, 2197, 2444
Cfr42I CCGCGG 1 cut(s) 2203
CseI GACGC 1 cut(s) 347
DdeI CTNAG 2 cut(s) 1902, 2475
DinI GGCGCC 1 cut(s) 334
DpnI GATC 9 cut(s) 84, 459, 590, 1258, 1376, 1738, 1797, 1811, 1995
DpnII GATC 9 cut(s) 82, 457, 588, 1256, 1374, 1736, 1795, 1809, 1993
EaeI YGGCCR 3 cut(s) 62, 307, 444
EagI CGGCCG 2 cut(s) 62, 444
Eam1104I CTCTTC 4 cut(s) 215, 227, 1142, 1878
EarI CTCTTC 4 cut(s) 215, 227, 1142, 1878
EciI GGCGGA 4 cut(s) 109, 175, 256, 1168
Ecl136II GAGCTC 1 cut(s) 1431
EclXI CGGCCG 2 cut(s) 62, 444
Eco105I TACGTA 1 cut(s) 2493
Eco130I CCWWGG 3 cut(s) 1263, 1814, 1961
Eco147I AGGCCT 1 cut(s) 53
Eco24I GRGCYC 1 cut(s) 1433
Eco31I GGTCTC 1 cut(s) 1273
Eco32I GATATC 1 cut(s) 1788
Eco47I GGWCC 6 cut(s) 32, 260, 887, 1867, 2197, 2444
Eco52I CGGCCG 2 cut(s) 62, 444
Eco53kI GAGCTC 1 cut(s) 1431
Eco57I CTGAAG 1 cut(s) 1295
EcoICRI GAGCTC 1 cut(s) 1431
EcoO109I RGGNCCY 1 cut(s) 260
EcoRII CCWGG 4 cut(s) 149, 628, 733, 1599
EcoRV GATATC 1 cut(s) 1788
EcoT14I CCWWGG 3 cut(s) 1263, 1814, 1961
EcoT22I ATGCAT 1 cut(s) 2068
EcoT38I GRGCYC 1 cut(s) 1433
EgeI GGCGCC 1 cut(s) 334
EheI GGCGCC 1 cut(s) 334
ErhI CCWWGG 3 cut(s) 1263, 1814, 1961
Esp3I CGTCTC 1 cut(s) 1755
FalI AAGNNNNNCTT 2 cut(s) 2162, 2194
FaqI GGGAC 6 cut(s) 110, 273, 454, 712, 1853, 2001
FauI CCCGC 1 cut(s) 329
FblI GTMKAC 1 cut(s) 2427
FokI GGATG 8 cut(s) 5, 149, 617, 998, 1112, 1694, 2382, 2453
FriOI GRGCYC 1 cut(s) 1433
FspBI CTAG 4 cut(s) 594, 969, 1442, 1962
FspI TGCGCA 1 cut(s) 2055
GlaI GCGC 2 cut(s) 334, 2055
GsaI CCCAGC 2 cut(s) 26, 919
GsuI CTGGAG 1 cut(s) 1095
HaeII RGCGCY 1 cut(s) 336
HaeIII GGCC 6 cut(s) 53, 64, 309, 378, 446, 1059
HapII CCGG 8 cut(s) 129, 257, 438, 850, 890, 1843, 1985, 2129
HgaI GACGC 1 cut(s) 347
HhaI GCGC 2 cut(s) 335, 2056
Hin1I GRCGYC 2 cut(s) 333, 339
Hin6I GCGC 2 cut(s) 333, 2054
HinP1I GCGC 2 cut(s) 333, 2054
HpaII CCGG 8 cut(s) 129, 257, 438, 850, 890, 1843, 1985, 2129
HphI GGTGA 1 cut(s) 952
Hpy166II GTNNAC 4 cut(s) 1105, 1107, 1190, 2428
Hpy188I TCNGA 9 cut(s) 72, 241, 637, 796, 901, 1428, 1809, 1836, 2002
Hpy188III TCNNGA 8 cut(s) 490, 1127, 1557, 1734, 1843, 1904, 2245, 2438
Hpy8I GTNNAC 4 cut(s) 1105, 1107, 1190, 2428
Hpy99I CGWCG 3 cut(s) 44, 341, 478
HpyAV CCTTC 9 cut(s) 208, 356, 931, 937, 1049, 1662, 1939, 2385, 2405
HpyCH4III ACNGT 7 cut(s) 703, 1311, 1552, 2242, 2370, 2425, 2432
HpyCH4IV ACGT 4 cut(s) 42, 1775, 2160, 2492
HpyF3I CTNAG 2 cut(s) 1902, 2475
HpySE526I ACGT 4 cut(s) 42, 1775, 2160, 2492
Hsp92I GRCGYC 2 cut(s) 333, 339
HspAI GCGC 2 cut(s) 333, 2054
KasI GGCGCC 1 cut(s) 332
KflI GGGWCCC 1 cut(s) 260
Kpn2I TCCGGA 1 cut(s) 1842
KspI CCGCGG 1 cut(s) 2203
Kzo9I GATC 9 cut(s) 82, 457, 588, 1256, 1374, 1736, 1795, 1809, 1993
Lsp1109I GCAGC 8 cut(s) 38, 359, 369, 381, 1030, 1845, 1873, 2313
LweI GCATC 5 cut(s) 17, 800, 964, 1730, 2259
MaeI CTAG 4 cut(s) 594, 969, 1442, 1962
MaeII ACGT 4 cut(s) 42, 1775, 2160, 2492
MaeIII GTNAC 2 cut(s) 623, 977
MalI GATC 9 cut(s) 84, 459, 590, 1258, 1376, 1738, 1797, 1811, 1995
MbiI CCGCTC 1 cut(s) 237
MboI GATC 9 cut(s) 82, 457, 588, 1256, 1374, 1736, 1795, 1809, 1993
MflI RGATCY 1 cut(s) 1736
MhlI GDGCHC 3 cut(s) 453, 1433, 1654
Mly113I GGCGCC 1 cut(s) 333
MlyI GAGTC 3 cut(s) 584, 839, 2309
MmeI TCCRAC 2 cut(s) 264, 879
Mph1103I ATGCAT 1 cut(s) 2068
MroI TCCGGA 1 cut(s) 1842
MseI TTAA 5 cut(s) 804, 924, 1095, 1581, 1620
MslI CAYNNNNRTG 1 cut(s) 659
MspA1I CMGCKG 3 cut(s) 372, 1864, 2202
MspCI CTTAAG 2 cut(s) 1580, 1619
MspI CCGG 8 cut(s) 129, 257, 438, 850, 890, 1843, 1985, 2129
MspR9I CCNGG 9 cut(s) 129, 151, 258, 439, 630, 735, 850, 1601, 1986
Mva1269I GAATGC 1 cut(s) 2066
MvaI CCWGG 4 cut(s) 151, 630, 735, 1601
MvnI CGCG 3 cut(s) 302, 312, 2202
NarI GGCGCC 1 cut(s) 333
NciI CCSGG 5 cut(s) 129, 258, 439, 850, 1986
NdeII GATC 9 cut(s) 82, 457, 588, 1256, 1374, 1736, 1795, 1809, 1993
NheI GCTAGC 1 cut(s) 968
NmeAIII GCCGAG 2 cut(s) 40, 432
NmuCI GTSAC 1 cut(s) 623
NsbI TGCGCA 1 cut(s) 2055
NsiI ATGCAT 1 cut(s) 2068
NspI RCATGY 4 cut(s) 256, 1305, 1495, 1858
PaeI GCATGC 1 cut(s) 256
PceI AGGCCT 1 cut(s) 53
PcsI WCGNNNNNNNCGW 1 cut(s) 473
PctI GAATGC 1 cut(s) 2066
PfeI GAWTC 7 cut(s) 427, 638, 1246, 1315, 1634, 1831, 1955
PleI GAGTC 3 cut(s) 583, 839, 2309
PluTI GGCGCC 1 cut(s) 336
PpsI GAGTC 3 cut(s) 583, 839, 2309
Ppu21I YACGTR 2 cut(s) 2161, 2493
PpuMI RGGWCCY 1 cut(s) 260
PsiI TTATAA 3 cut(s) 747, 1050, 1487
Psp124BI GAGCTC 1 cut(s) 1433
Psp5II RGGWCCY 1 cut(s) 260
Psp6I CCWGG 4 cut(s) 149, 628, 733, 1599
PspFI CCCAGC 2 cut(s) 22, 915
PspGI CCWGG 4 cut(s) 149, 628, 733, 1599
PspPI GGNCC 6 cut(s) 32, 260, 887, 1867, 2197, 2444
PspPPI RGGWCCY 1 cut(s) 260
PstI CTGCAG 1 cut(s) 1863
PsuI RGATCY 1 cut(s) 1736
PvuII CAGCTG 2 cut(s) 372, 1864
RseI CAYNNNNRTG 1 cut(s) 659
SacI GAGCTC 1 cut(s) 1433
SacII CCGCGG 1 cut(s) 2203
SaqAI TTAA 5 cut(s) 804, 924, 1095, 1581, 1620
Sau3AI GATC 9 cut(s) 82, 457, 588, 1256, 1374, 1736, 1795, 1809, 1993
Sau96I GGNCC 6 cut(s) 32, 260, 887, 1867, 2197, 2444
SchI GAGTC 3 cut(s) 584, 839, 2309
ScrFI CCNGG 9 cut(s) 129, 151, 258, 439, 630, 735, 850, 1601, 1986
SduI GDGCHC 3 cut(s) 453, 1433, 1654
SfaNI GCATC 5 cut(s) 17, 800, 964, 1730, 2259
SfcI CTRYAG 4 cut(s) 570, 1859, 2025, 2424
SfoI GGCGCC 1 cut(s) 334
Sfr303I CCGCGG 1 cut(s) 2203
SgrBI CCGCGG 1 cut(s) 2203
SinI GGWCC 6 cut(s) 32, 260, 887, 1867, 2197, 2444
SmiMI CAYNNNNRTG 1 cut(s) 659
SmlI CTYRAG 4 cut(s) 1432, 1580, 1619, 2245
SmoI CTYRAG 4 cut(s) 1432, 1580, 1619, 2245
SnaBI TACGTA 1 cut(s) 2493
SphI GCATGC 1 cut(s) 256
SseBI AGGCCT 1 cut(s) 53
SspDI GGCGCC 1 cut(s) 332
SspI AATATT 3 cut(s) 2060, 2082, 2337
SspMI CTAG 4 cut(s) 594, 969, 1442, 1962
SstI GAGCTC 1 cut(s) 1433
StuI AGGCCT 1 cut(s) 53
StyD4I CCNGG 9 cut(s) 127, 149, 256, 437, 628, 733, 848, 1599, 1984
StyI CCWWGG 3 cut(s) 1263, 1814, 1961
TaaI ACNGT 7 cut(s) 703, 1311, 1552, 2242, 2370, 2425, 2432
TaiI ACGT 4 cut(s) 45, 1778, 2163, 2495
TaqI TCGA 4 cut(s) 360, 489, 1135, 2046
TaqII GACCGA 1 cut(s) 49
TatI WGTACW 4 cut(s) 1104, 1710, 2172, 2361
TfiI GAWTC 7 cut(s) 427, 638, 1246, 1315, 1634, 1831, 1955
Tru1I TTAA 5 cut(s) 804, 924, 1095, 1581, 1620
Tru9I TTAA 5 cut(s) 804, 924, 1095, 1581, 1620
TscAI CASTG 6 cut(s) 332, 1071, 1114, 1208, 1278, 1357
TseFI GTSAC 1 cut(s) 623
TseI GCWGC 8 cut(s) 26, 369, 372, 382, 1043, 1858, 1861, 2326
Tsp45I GTSAC 1 cut(s) 623
TspDTI ATGAA 7 cut(s) 114, 202, 831, 1921, 2097, 2297, 2384
TspGWI ACGGA 3 cut(s) 411, 998, 1387
TspRI CASTG 6 cut(s) 332, 1071, 1114, 1208, 1278, 1357
Vha464I CTTAAG 2 cut(s) 1580, 1619
VpaK11BI GGWCC 6 cut(s) 32, 260, 887, 1867, 2197, 2444
XceI RCATGY 4 cut(s) 256, 1305, 1495, 1858
XmaJI CCTAGG 1 cut(s) 1961
XmiI GTMKAC 1 cut(s) 2427
XspI CTAG 4 cut(s) 594, 969, 1442, 1962
Zsp2I ATGCAT 1 cut(s) 2068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.