pycom02g09710

Calcium-dependent channel, 7TM region, putative phosphate

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0015059
Physical Location & Seq
Forward (+)
6719311 .. 6721854
2544 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g09710.1

Sequence Viewer

Length: 2544 bp
ATGAAGAAATTCCAATCCAATTCAATCCCAATTACCTCAAATCCCACCAGAAAAATCCTAATTTCCGGTTCTTCAATCATTAGATTTCCCATGCCTCCGATGGCCACGCCTTATCAATCTTCAGTCTTCTCCCCAATTCCCTCCATGAACGACACTCTCTCTCCTCCGCCGTCCCCCGGCGACGGCGCCGATACCTACGAGGCCTGGTACGGCAACATCCAGTACCTCCTCAACATCTCCGCCATCGGCTCCTTCTTCTGCGTCTTCTTCTTCATATTTGTCAAGCTTCGCAGCGACCACCGCCGCATGCCCGGCCCGTCTGCCCTCGTCACTAAGCTCCTCGCGGTGTGGCACGCCACGTGTCGTGAGATTGCGCGCCACTGTGGCGCTGACGCCGCCCAGTTTCTCTTGATCGAGGGCGGCAGCTGCGGGTTGCTGTTGTCCATGGCGGTTTTGGCTGTGCTTGTGATGCTTCCGCTGAATCTCTACGCTGGGAGTGCTGTGCTGGGGGATCAATTCTCGAAGACGACCATCAATCACATTGAGAAAGGTTCGGCTTTGCTTTGGGTGCATTTCGTTTTCGTGGTTGTTGTTGTTGTTCTTGTGCATTTTGGTATTTCTGCTATTGAAGGGAGATTGAGAATCACTAGGATTAGGGATGGGAATGGCAATTTGAGTGACCCGACTGTGAATTCCACGGCGATTTTTACTATAATGGTGCAGGGGATACCGAAAACCATAGGGAATGATGGGACTGTGCTGCATGAGTATTTTCAACATAGGTATCCTGGAAAGGTTTATAGGGTTATAATGCCGATGGATTTGTGTGCTTTGGATGAATTAGCCTCGGAATTAGTGAGGGTTAGGCATGAAATTTCTTGGTTGGTTGCACGAATTGACTCTCGGCTCTTGCCGTATGAAAGTGAAGAGAGTGGATACGTAAGGACTTCTTCACAGGGAGTATGGGGTTGTGCTTGTAATTTATGGCGAAAGGTGGAGGATTTCTGGTATCATACTATGGCTAGGTTGGGATACACTGATGAAAGGAAGTTAGGAGAGTTGCAGGGATGGAGAGCTGAATTGGAGACTGAACTGGCAGCTTACAAAGAAGGTCGTGCACTAGGTGCTGGAGTTGCGTTTGTGGTGTTTAAGGATGTGTACACTGCTAATAAAGCGGTTCAGGACTTTCGACATGAGAAGAAGAGGCGGATAGGGAAATTATTTTCTCTTGTGGAATCGCGTTTACAGAGAAACCAATGGAAAGTTGAGCAGGCACCCTTGGCCACAGATATTTATTGGAATCATTTGGGGTCGTCGAAGCTCTCACTGAAATTGCGAAGAGTGTTGGTGAACACTTGCTTGTTGTTGATTCTTCTGTTCTTTAGCTCTCCTCTGGCAATTATCAGTGCTCTAAAAAATGCCTGGCGGATTATAAATGCGGAAGCTATGGATAATGCCCAGTTGTGGTTTGCTTGGGTGCAGAGCTCAAGCTGGCTTGGGAGTCTTATCTTTCAGTTTCTACCCAATGTGTTTATTTTTGTCAGCATGTATATGGTTATTCCATCAGCACTTTCATATCTCTCCAAGTTCGAGCGACATCTTACAGTGTCGGGGGAGCAACGAGCTGCTCTTCTTAAGATGGTTTGTTTCTTCCTGGTGAATCTTATACTTCTTAAGGGACTGGTTGAATCATCTTTAGAGAGTGCCATCTTAAAAATGGGAAGGTGCTATATGGATGGAGAAGATTGCAAGAGAATTGAGCAGTACATGAGTGCCTCGTTTTTGTCAAGATCGTGTCTTTCTTCTCTTGCATTCTTGATAACAAGTACATTCCTGGGCATATCTTATGATCTATTGGCTCCAATCCCTTGGATAAAAAGGAAGATTCAGAAGTTTCGGAAGAATGACATGCTACAGCTTGTCCCGGAACAAAATGAAGAATACGCATTGGAAACTCAGGAAACAAATGGTCTAGAAAGGCCCTTGATTGTGGACAACACTTACGATTCTCCCAGAACTCTGCAGGGAAATGATCTCTCTGATTACCCAATCAACAGAACATCAACTGCCCCCAAACAGACATTTGATTTCGCCCAATATTATGCATTCAATCTGACGATATTTGCCCTGACCTTCATATATTCTTCCTTCTCCCCACTTGTGGTTCCCGTTGGTGCTATTTATTTTGGGTATAGGTACGTGGTCGACAAGTACAACTTCCTCTTTGTCTATAGGGTCCGGGGCTTTCCTGCTGGGAATGATGGGAAGTTGATGGATACAGTTCTGTGCATCATGCGTTTTTGCGTCGATTTATTCCTGTTGGCAATGCTGTTGTTCTTTTCGGTCCACGGAGACTCAACGAAGCTGCAAGCTATATTCACACTTGGGGTATTAGTAATGTATAAATTGTTGCCTTCCCAGAATGACAGTCTTCATCCAGCACTTTTGGAAGGCGTACAAACTGTAGACAGTGTTGTGGATGGACCTATAGATTATGAGGTGTTTTCACAGCCCAAGTTTGACTGGGATACATATTATTCATGA

Protein Analysis

848

Amino Acids

95.77

Weight (kDa)

8.46

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RSN1_TM PF13967 99 - 205 5.9e-11 Late exocytosis, associated with Golgi transport
PHM7_cyt PF14703 236 - 435 4.6e-12 Cytosolic domain of 10TM putative phosphate transporter
RSN1_7TM PF02714 446 - 631 5.4e-11 Calcium-dependent channel, 7TM region, putative phosphate
RSN1_7TM PF02714 692 - 780 9.6e-12 Calcium-dependent channel, 7TM region, putative phosphate
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015059)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G35870
fragaria_vesca FvH4_1g11170
malus_domestica MD02G1124200.v1.1 MD15G1238200.v1.1
prunus_persica Prupe.7G174200_v2.0.a1
pyrus_communis pycom02g09710 pycom05g15420 pycom15g21190
rosa_chinensis RchiOBHm_Chr2g0098461
rosa_laevigata RLG00000016800
rosa_multiflora Rmu_sc0010938.1_g000006
rosa_roxburghii Rroxscaffold_2G00143940
rosa_rugosa Rorug02G0073400
rosa_samantha Rh2AG120700 Rh2DG125700
rosa_wichuraiana Rw2G009460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 809, 1433
AccB1I GGYRCC 2 cut(s) 185, 1273
AccI GTMKAC 2 cut(s) 2205, 2466
AccII CGCG 3 cut(s) 344, 376, 1240
AclWI GGATC 1 cut(s) 519
AcoI YGGCCR 2 cut(s) 102, 1281
AcsI RAATTY 3 cut(s) 8, 691, 873
AcuI CTGAAG 1 cut(s) 105
AcvI CACGTG 1 cut(s) 360
AcyI GRCGYC 2 cut(s) 186, 393
AdeI CACNNNGTG 1 cut(s) 1124
AfaI GTAC 8 cut(s) 209, 224, 1160, 1766, 1828, 2198, 2213, 2457
AfiI CCNNNNNNNGG 4 cut(s) 176, 182, 1464, 2161
AflII CTTAAG 2 cut(s) 1634, 1673
AflIII ACRYGT 1 cut(s) 359
AgsI TTSAA 6 cut(s) 24, 75, 629, 776, 1688, 2110
AjnI CCWGG 5 cut(s) 203, 787, 1421, 1653, 1833
Alw21I GWGCWC 3 cut(s) 1120, 1411, 1487
Alw26I GTCTC 2 cut(s) 1079, 2346
Alw44I GTGCAC 1 cut(s) 1116
AlwI GGATC 1 cut(s) 519
AoxI GGCC 5 cut(s) 102, 201, 313, 1281, 1979
ApaLI GTGCAC 1 cut(s) 1116
ApeKI GCWGC 7 cut(s) 291, 423, 426, 760, 1097, 1625, 2365
ApoI RAATTY 3 cut(s) 8, 691, 873
Asp700I GAANNNNTTC 1 cut(s) 8
AspLEI GCGC 4 cut(s) 188, 376, 378, 389
AspS9I GGNCC 5 cut(s) 314, 1980, 2236, 2344, 2483
AsuC2I CCSGG 4 cut(s) 177, 312, 1925, 2240
AsuHPI GGTGA 2 cut(s) 1360, 1669
AvaII GGWCC 3 cut(s) 2236, 2344, 2483
BaeGI GKGCMC 1 cut(s) 1120
BaeI ACNNNNGTAYC 2 cut(s) 767, 800
BalI TGGCCA 2 cut(s) 104, 1283
BanI GGYRCC 2 cut(s) 185, 1273
BanII GRGCYC 1 cut(s) 1487
BbrPI CACGTG 1 cut(s) 360
BbsI GAAGAC 4 cut(s) 118, 256, 530, 2423
Bbv12I GWGCWC 3 cut(s) 1120, 1411, 1487
BbvI GCAGC 7 cut(s) 303, 413, 435, 747, 1109, 1612, 2352
BceAI ACGGC 5 cut(s) 154, 199, 226, 714, 898
BciT130I CCWGG 5 cut(s) 205, 789, 1423, 1655, 1835
BciVI GTATCC 6 cut(s) 720, 795, 929, 1025, 2269, 2521
BcnI CCSGG 4 cut(s) 177, 312, 1925, 2240
BcoDI GTCTC 2 cut(s) 1079, 2346
BfaI CTAG 4 cut(s) 648, 1023, 1121, 1973
BfmI CTRYAG 5 cut(s) 1913, 2021, 2230, 2463, 2487
BfoI RGCGCY 2 cut(s) 189, 390
BfrI CTTAAG 2 cut(s) 1634, 1673
BfuI GTATCC 6 cut(s) 720, 795, 929, 1025, 2269, 2521
BglI GCCNNNNNGGC 1 cut(s) 384
Bme1390I CCNGG 9 cut(s) 177, 205, 312, 789, 1423, 1655, 1835, 1925, 2240
Bme18I GGWCC 3 cut(s) 2236, 2344, 2483
BmgT120I GGNCC 5 cut(s) 314, 1980, 2236, 2344, 2483
BmiI GGNNCC 6 cut(s) 187, 250, 1275, 1860, 2166, 2237
BmrFI CCNGG 9 cut(s) 177, 205, 312, 789, 1423, 1655, 1835, 1925, 2240
BmrI ACTGGG 3 cut(s) 394, 1453, 2533
BmsI GCATC 2 cut(s) 459, 2298
BmuI ACTGGG 3 cut(s) 394, 1453, 2533
BpiI GAAGAC 4 cut(s) 118, 256, 530, 2423
BpmI CTGGAG 1 cut(s) 1149
BpuEI CTTGAG 1 cut(s) 1471
BpuMI CCSGG 4 cut(s) 177, 312, 1925, 2240
BsaAI YACGTR 3 cut(s) 360, 940, 2200
BsaHI GRCGYC 2 cut(s) 186, 393
BsaJI CCNNGG 9 cut(s) 175, 444, 696, 846, 1278, 1834, 1868, 2239, 2347
BsaWI WCCGGW 1 cut(s) 65
BsaXI ACNNNNNCTCC 2 cut(s) 145, 175
Bsc4I CCNNNNNNNGG 4 cut(s) 176, 182, 1464, 2161
Bse1I ACTGG 6 cut(s) 220, 400, 1098, 1459, 1686, 2528
Bse3DI GCAATG 1 cut(s) 2331
BseBI CCWGG 5 cut(s) 205, 789, 1423, 1655, 1835
BseDI CCNNGG 9 cut(s) 175, 444, 696, 846, 1278, 1834, 1868, 2239, 2347
BseGI GGATG 8 cut(s) 216, 664, 841, 1073, 1159, 1741, 2434, 2485
BseLI CCNNNNNNNGG 4 cut(s) 176, 182, 1464, 2161
BseMI GCAATG 1 cut(s) 2331
BseMII CTCAG 1 cut(s) 1970
BseNI ACTGG 6 cut(s) 220, 400, 1098, 1459, 1686, 2528
BsePI GCGCGC 1 cut(s) 374
BseRI GAGGAG 4 cut(s) 153, 218, 329, 1380
BseSI GKGCMC 1 cut(s) 1120
BseXI GCAGC 7 cut(s) 303, 413, 435, 747, 1109, 1612, 2352
BseYI CCCAGC 3 cut(s) 491, 505, 2252
BsgI GTGCAG 2 cut(s) 740, 1499
Bsh1236I CGCG 3 cut(s) 344, 376, 1240
BshFI GGCC 5 cut(s) 104, 203, 315, 1283, 1981
BshNI GGYRCC 2 cut(s) 185, 1273
BsiHKAI GWGCWC 3 cut(s) 1120, 1411, 1487
BsiSI CCGG 5 cut(s) 66, 177, 312, 1925, 2239
BslFI GGGAC 4 cut(s) 157, 766, 1692, 1907
BslI CCNNNNNNNGG 4 cut(s) 176, 182, 1464, 2161
BsmAI GTCTC 2 cut(s) 1079, 2346
BsmFI GGGAC 4 cut(s) 157, 766, 1692, 1907
BsmI GAATGC 2 cut(s) 1811, 2105
BsnI GGCC 5 cut(s) 104, 203, 315, 1283, 1981
Bsp1286I GDGCHC 3 cut(s) 1120, 1411, 1487
Bsp1407I TGTACA 1 cut(s) 1158
Bsp143I GATC 5 cut(s) 411, 511, 1790, 1849, 2032
Bsp19I CCATGG 1 cut(s) 444
BspANI GGCC 5 cut(s) 104, 203, 315, 1283, 1981
BspCNI CTCAG 1 cut(s) 1969
BspFNI CGCG 3 cut(s) 344, 376, 1240
BspHI TCATGA 1 cut(s) 2540
BspLI GGNNCC 6 cut(s) 187, 250, 1275, 1860, 2166, 2237
BspMAI CTGCAG 1 cut(s) 2025
BspPI GGATC 1 cut(s) 519
BspQI GCTCTTC 1 cut(s) 1635
BspT107I GGYRCC 2 cut(s) 185, 1273
BspTI CTTAAG 2 cut(s) 1634, 1673
BsrDI GCAATG 1 cut(s) 2331
BsrGI TGTACA 1 cut(s) 1158
BsrI ACTGG 6 cut(s) 220, 400, 1098, 1459, 1686, 2528
BssECI CCNNGG 9 cut(s) 175, 444, 696, 846, 1278, 1834, 1868, 2239, 2347
BssHII GCGCGC 1 cut(s) 374
BssMI GATC 5 cut(s) 411, 511, 1790, 1849, 2032
BssNI GRCGYC 2 cut(s) 186, 393
BssT1I CCWWGG 3 cut(s) 444, 1278, 1868
Bst2UI CCWGG 5 cut(s) 205, 789, 1423, 1655, 1835
Bst4CI ACNGT 8 cut(s) 383, 688, 757, 1606, 2281, 2429, 2464, 2471
Bst6I CTCTTC 4 cut(s) 921, 1196, 1333, 1635
BstACI GRCGYC 2 cut(s) 186, 393
BstAFI CTTAAG 2 cut(s) 1634, 1673
BstAPI GCANNNNNTGC 1 cut(s) 1124
BstAUI TGTACA 1 cut(s) 1158
BstBAI YACGTR 3 cut(s) 360, 940, 2200
BstC8I GCNNGC 6 cut(s) 308, 354, 376, 1272, 1493, 2370
BstDEI CTNAG 2 cut(s) 333, 1956
BstDSI CCRYGG 3 cut(s) 444, 696, 2347
BstF5I GGATG 8 cut(s) 216, 664, 841, 1073, 1159, 1741, 2434, 2485
BstFNI CGCG 3 cut(s) 344, 376, 1240
BstH2I RGCGCY 2 cut(s) 189, 390
BstHHI GCGC 4 cut(s) 188, 376, 378, 389
BstKTI GATC 5 cut(s) 414, 514, 1793, 1852, 2035
BstMAI GTCTC 2 cut(s) 1079, 2346
BstMBI GATC 5 cut(s) 411, 511, 1790, 1849, 2032
BstNI CCWGG 5 cut(s) 205, 789, 1423, 1655, 1835
BstNSI RCATGY 3 cut(s) 310, 1549, 1912
BstSCI CCNGG 9 cut(s) 175, 203, 310, 787, 1421, 1653, 1833, 1923, 2238
BstSFI CTRYAG 5 cut(s) 1913, 2021, 2230, 2463, 2487
BstSLI GKGCMC 1 cut(s) 1120
BstSNI TACGTA 1 cut(s) 940
BstUI CGCG 3 cut(s) 344, 376, 1240
BstV1I GCAGC 7 cut(s) 303, 413, 435, 747, 1109, 1612, 2352
BstV2I GAAGAC 4 cut(s) 118, 256, 530, 2423
BstXI CCANNNNNNTGG 1 cut(s) 1869
BsuI GTATCC 6 cut(s) 720, 795, 929, 1025, 2269, 2521
BsuRI GGCC 5 cut(s) 104, 203, 315, 1283, 1981
BtgI CCRYGG 3 cut(s) 444, 696, 2347
BtsCI GGATG 8 cut(s) 216, 664, 841, 1073, 1159, 1741, 2434, 2485
BtsI GCAGTG 1 cut(s) 1161
BtsIMutI CAGTG 7 cut(s) 379, 1035, 1161, 1325, 1411, 1611, 2476
Cac8I GCNNGC 6 cut(s) 308, 354, 376, 1272, 1493, 2370
CciI TCATGA 1 cut(s) 2540
CfoI GCGC 4 cut(s) 188, 376, 378, 389
Cfr13I GGNCC 5 cut(s) 314, 1980, 2236, 2344, 2483
CseI GACGC 3 cut(s) 250, 401, 2293
Csp6I GTAC 8 cut(s) 208, 223, 1159, 1765, 1827, 2197, 2212, 2456
CviQI GTAC 8 cut(s) 208, 223, 1159, 1765, 1827, 2197, 2212, 2456
DdeI CTNAG 2 cut(s) 333, 1956
DinI GGCGCC 1 cut(s) 187
DpnI GATC 5 cut(s) 413, 513, 1792, 1851, 2034
DpnII GATC 5 cut(s) 411, 511, 1790, 1849, 2032
DraIII CACNNNGTG 1 cut(s) 1124
EaeI YGGCCR 2 cut(s) 102, 1281
Eam1104I CTCTTC 4 cut(s) 921, 1196, 1333, 1635
EarI CTCTTC 4 cut(s) 921, 1196, 1333, 1635
EciI GGCGGA 4 cut(s) 156, 229, 1222, 1441
Ecl136II GAGCTC 1 cut(s) 1485
Eco105I TACGTA 1 cut(s) 940
Eco130I CCWWGG 3 cut(s) 444, 1278, 1868
Eco147I AGGCCT 1 cut(s) 203
Eco24I GRGCYC 1 cut(s) 1487
Eco47I GGWCC 3 cut(s) 2236, 2344, 2483
Eco53kI GAGCTC 1 cut(s) 1485
Eco57I CTGAAG 1 cut(s) 105
Eco72I CACGTG 1 cut(s) 360
EcoICRI GAGCTC 1 cut(s) 1485
EcoO109I RGGNCCY 1 cut(s) 1980
EcoRI GAATTC 1 cut(s) 691
EcoRII CCWGG 5 cut(s) 203, 787, 1421, 1653, 1833
EcoT14I CCWWGG 3 cut(s) 444, 1278, 1868
EcoT22I ATGCAT 1 cut(s) 2107
EcoT38I GRGCYC 1 cut(s) 1487
EgeI GGCGCC 1 cut(s) 187
EheI GGCGCC 1 cut(s) 187
ErhI CCWWGG 3 cut(s) 444, 1278, 1868
FalI AAGNNNNNCTT 4 cut(s) 934, 966, 2201, 2233
FaqI GGGAC 4 cut(s) 157, 766, 1692, 1907
FauI CCCGC 1 cut(s) 422
FblI GTMKAC 2 cut(s) 2205, 2466
FokI GGATG 8 cut(s) 203, 671, 848, 1080, 1166, 1748, 2421, 2492
FriOI GRGCYC 1 cut(s) 1487
FspBI CTAG 4 cut(s) 648, 1023, 1121, 1973
GlaI GCGC 4 cut(s) 187, 375, 377, 388
GsaI CCCAGC 3 cut(s) 495, 509, 2256
GsuI CTGGAG 1 cut(s) 1149
HaeII RGCGCY 2 cut(s) 189, 390
HaeIII GGCC 5 cut(s) 104, 203, 315, 1283, 1981
HapII CCGG 5 cut(s) 66, 177, 312, 1925, 2239
HgaI GACGC 3 cut(s) 250, 401, 2293
HhaI GCGC 4 cut(s) 188, 376, 378, 389
Hin1I GRCGYC 2 cut(s) 186, 393
Hin6I GCGC 4 cut(s) 186, 374, 376, 387
HinP1I GCGC 4 cut(s) 186, 374, 376, 387
HincII GTYRAC 1 cut(s) 2206
HindII GTYRAC 1 cut(s) 2206
HindIII AAGCTT 1 cut(s) 284
HpaII CCGG 5 cut(s) 66, 177, 312, 1925, 2239
HphI GGTGA 2 cut(s) 1360, 1669
Hpy166II GTNNAC 9 cut(s) 1118, 1159, 1161, 1244, 1351, 1993, 2206, 2347, 2467
Hpy188I TCNGA 6 cut(s) 99, 850, 1890, 1899, 2041, 2115
Hpy188III TCNNGA 9 cut(s) 365, 409, 520, 1181, 1788, 1816, 1958, 1973, 2541
Hpy8I GTNNAC 9 cut(s) 1118, 1159, 1161, 1244, 1351, 1993, 2206, 2347, 2467
Hpy99I CGWCG 3 cut(s) 185, 1318, 2309
HpyAV CCTTC 8 cut(s) 262, 623, 1103, 1716, 2143, 2158, 2424, 2444
HpyCH4III ACNGT 8 cut(s) 383, 688, 757, 1606, 2281, 2429, 2464, 2471
HpyCH4IV ACGT 3 cut(s) 359, 939, 2199
HpyF3I CTNAG 2 cut(s) 333, 1956
HpySE526I ACGT 3 cut(s) 359, 939, 2199
Hsp92I GRCGYC 2 cut(s) 186, 393
HspAI GCGC 4 cut(s) 186, 374, 376, 387
KasI GGCGCC 1 cut(s) 185
Kzo9I GATC 5 cut(s) 411, 511, 1790, 1849, 2032
LguI GCTCTTC 1 cut(s) 1635
LmnI GCTCC 4 cut(s) 254, 342, 1615, 1864
Lsp1109I GCAGC 7 cut(s) 303, 413, 435, 747, 1109, 1612, 2352
LweI GCATC 2 cut(s) 459, 2298
MaeI CTAG 4 cut(s) 648, 1023, 1121, 1973
MaeII ACGT 3 cut(s) 359, 939, 2199
MaeIII GTNAC 2 cut(s) 328, 677
MalI GATC 5 cut(s) 413, 513, 1792, 1851, 2034
MboI GATC 5 cut(s) 411, 511, 1790, 1849, 2032
MhlI GDGCHC 3 cut(s) 1120, 1411, 1487
MlsI TGGCCA 2 cut(s) 104, 1283
MluNI TGGCCA 2 cut(s) 104, 1283
Mly113I GGCGCC 1 cut(s) 186
MlyI GAGTC 3 cut(s) 893, 1510, 2348
Mox20I TGGCCA 2 cut(s) 104, 1283
Mph1103I ATGCAT 1 cut(s) 2107
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 2 cut(s) 104, 1283
MseI TTAA 4 cut(s) 1149, 1635, 1674, 1712
MslI CAYNNNNRTG 1 cut(s) 1550
Msp20I TGGCCA 2 cut(s) 104, 1283
MspA1I CMGCKG 2 cut(s) 426, 478
MspCI CTTAAG 2 cut(s) 1634, 1673
MspI CCGG 5 cut(s) 66, 177, 312, 1925, 2239
MspR9I CCNGG 9 cut(s) 177, 205, 312, 789, 1423, 1655, 1835, 1925, 2240
Mva1269I GAATGC 2 cut(s) 1811, 2105
MvaI CCWGG 5 cut(s) 205, 789, 1423, 1655, 1835
MvnI CGCG 3 cut(s) 344, 376, 1240
NarI GGCGCC 1 cut(s) 186
NciI CCSGG 4 cut(s) 177, 312, 1925, 2240
NcoI CCATGG 1 cut(s) 444
NdeII GATC 5 cut(s) 411, 511, 1790, 1849, 2032
NlaIV GGNNCC 6 cut(s) 187, 250, 1275, 1860, 2166, 2237
NmeAIII GCCGAG 1 cut(s) 883
NmuCI GTSAC 2 cut(s) 328, 677
NsiI ATGCAT 1 cut(s) 2107
NspI RCATGY 3 cut(s) 310, 1549, 1912
PaeI GCATGC 1 cut(s) 310
PagI TCATGA 1 cut(s) 2540
PauI GCGCGC 1 cut(s) 374
PceI AGGCCT 1 cut(s) 203
PciSI GCTCTTC 1 cut(s) 1635
PctI GAATGC 2 cut(s) 1811, 2105
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 9 cut(s) 481, 642, 1235, 1300, 1369, 1660, 1688, 1885, 2006
PfoI TCCNGGA 2 cut(s) 787, 1923
PleI GAGTC 3 cut(s) 893, 1509, 2348
PluTI GGCGCC 1 cut(s) 189
PmaCI CACGTG 1 cut(s) 360
PmlI CACGTG 1 cut(s) 360
PpsI GAGTC 3 cut(s) 893, 1509, 2348
Ppu21I YACGTR 3 cut(s) 360, 940, 2200
PsiI TTATAA 2 cut(s) 809, 1433
Psp124BI GAGCTC 1 cut(s) 1487
Psp6I CCWGG 5 cut(s) 203, 787, 1421, 1653, 1833
PspCI CACGTG 1 cut(s) 360
PspFI CCCAGC 3 cut(s) 491, 505, 2252
PspGI CCWGG 5 cut(s) 203, 787, 1421, 1653, 1833
PspN4I GGNNCC 6 cut(s) 187, 250, 1275, 1860, 2166, 2237
PspPI GGNCC 5 cut(s) 314, 1980, 2236, 2344, 2483
PstI CTGCAG 1 cut(s) 2025
PteI GCGCGC 1 cut(s) 374
PvuII CAGCTG 1 cut(s) 426
RsaI GTAC 8 cut(s) 209, 224, 1160, 1766, 1828, 2198, 2213, 2457
RsaNI GTAC 8 cut(s) 208, 223, 1159, 1765, 1827, 2197, 2212, 2456
RseI CAYNNNNRTG 1 cut(s) 1550
SacI GAGCTC 1 cut(s) 1487
SalI GTCGAC 1 cut(s) 2204
SapI GCTCTTC 1 cut(s) 1635
SaqAI TTAA 4 cut(s) 1149, 1635, 1674, 1712
Sau3AI GATC 5 cut(s) 411, 511, 1790, 1849, 2032
Sau96I GGNCC 5 cut(s) 314, 1980, 2236, 2344, 2483
SchI GAGTC 3 cut(s) 893, 1510, 2348
ScrFI CCNGG 9 cut(s) 177, 205, 312, 789, 1423, 1655, 1835, 1925, 2240
SduI GDGCHC 3 cut(s) 1120, 1411, 1487
SfaNI GCATC 2 cut(s) 459, 2298
SfcI CTRYAG 5 cut(s) 1913, 2021, 2230, 2463, 2487
SfoI GGCGCC 1 cut(s) 187
SinI GGWCC 3 cut(s) 2236, 2344, 2483
SmiMI CAYNNNNRTG 1 cut(s) 1550
SmlI CTYRAG 3 cut(s) 1486, 1634, 1673
SmoI CTYRAG 3 cut(s) 1486, 1634, 1673
SnaBI TACGTA 1 cut(s) 940
SphI GCATGC 1 cut(s) 310
SseBI AGGCCT 1 cut(s) 203
SspDI GGCGCC 1 cut(s) 185
SspI AATATT 1 cut(s) 2099
SspMI CTAG 4 cut(s) 648, 1023, 1121, 1973
SstI GAGCTC 1 cut(s) 1487
StuI AGGCCT 1 cut(s) 203
StyD4I CCNGG 9 cut(s) 175, 203, 310, 787, 1421, 1653, 1833, 1923, 2238
StyI CCWWGG 3 cut(s) 444, 1278, 1868
TaaI ACNGT 8 cut(s) 383, 688, 757, 1606, 2281, 2429, 2464, 2471
TaiI ACGT 3 cut(s) 362, 942, 2202
TaqI TCGA 7 cut(s) 414, 521, 1189, 1316, 1590, 2205, 2307
TaqII GACCGA 1 cut(s) 2332
TatI WGTACW 4 cut(s) 1158, 1764, 1826, 2211
TauI GCSGC 3 cut(s) 306, 398, 423
TfiI GAWTC 9 cut(s) 481, 642, 1235, 1300, 1369, 1660, 1688, 1885, 2006
Tru1I TTAA 4 cut(s) 1149, 1635, 1674, 1712
Tru9I TTAA 4 cut(s) 1149, 1635, 1674, 1712
TscAI CASTG 7 cut(s) 386, 1042, 1168, 1332, 1411, 1611, 2476
TseFI GTSAC 2 cut(s) 328, 677
TseI GCWGC 7 cut(s) 291, 423, 426, 760, 1097, 1625, 2365
Tsp45I GTSAC 2 cut(s) 328, 677
TspGWI ACGGA 1 cut(s) 2364
TspRI CASTG 7 cut(s) 386, 1042, 1168, 1332, 1411, 1611, 2476
Vha464I CTTAAG 2 cut(s) 1634, 1673
VneI GTGCAC 1 cut(s) 1116
VpaK11BI GGWCC 3 cut(s) 2236, 2344, 2483
XapI RAATTY 3 cut(s) 8, 691, 873
XbaI TCTAGA 1 cut(s) 1972
XceI RCATGY 3 cut(s) 310, 1549, 1912
XcmI CCANNNNNNNNNTGG 3 cut(s) 97, 451, 1714
XmiI GTMKAC 2 cut(s) 2205, 2466
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 4 cut(s) 648, 1023, 1121, 1973
Zsp2I ATGCAT 1 cut(s) 2107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.