MD02G1132100.v1.1

Calcium-dependent protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006979
Physical Location & Seq
Reverse (-)
10642928 .. 10647669
4742 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1132100.v1.1.491

Sequence Viewer

Length: 1671 bp
ATGGGAATTTGCTTCTCCGCCGTCAAGGTCAGTGGCTCAAATAGCAACAACAACGCCCACGGCACCGCCGCCTCCAATCACAACCGGAAACAGAGGACAACAGCCGGGAAGCAGGAACAGAGACCCACCCACAGACCCAATGAGCCGCCCAAGAGGCAGGCTGTTCATAAACCTAAAACCAAGCTCAACTCCAGGAGCCGGAAGGGGGCCGTTCCCTGCGGAAAGCGGACCGATTTCGGGTACGATAAGAATTTCGATAAGCGGTACACGATCGGGAAGTTGTTGGGTCATGGGCAATTTGGGTACACGTATGTTGCAACCGATAGGGCAAATGGGGATCGAGTTGCGGTCAAGAGGATTGATAAGAACAAGATGATTCTACCTATTGCTGTTGAAGATGTTAAGCGAGAGGTCAGGATATTACAGGAGCTTGCAGGCCATGAGAATGTGGTCCATTTTTATGATGCATTTGAAGATGAATCTTATGTTTATATAGTTATGGAGTTATGTGAAGGTGGTGAGTTGCTGGATCGGATTTTAGAGAAGAAGGACAGCCGTTACACTGAAAAAGATGCTGCAGTAGTTGTAAGGCAGATGCTCAAGGTTGCAGCTGAGTGTCATTTACATGGTCTGGTGCACCGTGACATGAAGCCTGAGAATTTTCTTTTCAAGTCAAAATCCTTAGACTCACCTTTGAAGGCCACAGACTTTGGTTTGTCTGACTTCATAAAACCAGGAAAGAAGTTTCAAGATATTGTTGGCAGTGCTTACTATGTTGCTCCTGAAGTATTGAGACGCAAGTCAGGGCCTGAGTCAGATGTTTGGAGTATTGGCGTTATTACCTACATTTTGCTTTGTGGGAGGCGTCCATTTTGGGATAAGACCGAAGATGGTATATTTAAGGAGGTCTTAAGGAACAAGCCTGATTTTCGTCGCAAACCATGGCCAAGCATAAGTAACAGTGCTAAAGATTTTGTTAAGAAGTTACTTGTGAAGGATCCTCGGGCGAGGCTAACTGCTGCTCAGGCACTATCACACCCGTGGGTTAGAGAAGGGGGGAATGCATCAGAGATTCCTATCGATATATCCGTTCTCAACAACATGCGCCAGTTCGTGAAATATAGTCGTTTGAAACAGTTTGCTCTAAGGGCATTAGCTAGCACACTTAATCCCGAGGAGCTGTCTGATCTCAAAGATCAATTTGATGCAATTGACGTGGATAAGAATGGTTCTATTAGTCTTGAAGAAATGAGACAGGCGCTTGCTAAAGATGTCCCTTGGAAATTGAAAGATTCGCGTGTTCTTGAGATACTGGAAGCGATTGACATCAACACCGACGGGCTTGTGGACTTCACTGAGTTTGTCGCGGCTACTCTACATGTGCATCAATTGGAGGAACATGACTCAGAGAAGTGGCTGCAGCGCTCACGGACAGCTTTTGAGAAATTCGACATAGATAAAGATGGGTTCATAACTCCGGAGGAACTTAGAATGCACACTGGTCTAAAAGGTTCTATTGATCCACTTCTTGAAGAGGCGGACATCGACAAAGATGGGAGGATCAGCCTATCAGAATTCCGCAGACTTCTAAGAACTGCAAGCATTAGCGCGAGAATTGTGACTAGCCCACCTGGTCACCGGAATGATAGGAAGGTACAAGATTGGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002831 GO:0002832 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0004721 GO:0004722 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016788 GO:0016791 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022603 GO:0023052 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043900 GO:0043901 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0048519 GO:0048583 GO:0048585 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060688 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080134 GO:0097305 GO:0097306 GO:0098542 GO:0140096 GO:1900424 GO:1900425 GO:1900618 GO:1901564 GO:1901700 GO:1901701 GO:1905428 GO:2000026 GO:2000032
KEGG Pathways
Metabolic & Signaling
ko04626 ko05145 map04626 map05145
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 Pkinase

Protein Analysis

557

Amino Acids

63.26

Weight (kDa)

9.17

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 89 - 349 2.8e-71 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 91 - 342 6.1e-29 Protein tyrosine and serine/threonine kinase
EF-hand_7 PF13499 395 - 457 2.6e-12 EF-hand domain pair
EF-hand_5 PF13202 397 - 419 7e-06 EF hand
EF-hand_7 PF13499 475 - 530 5.2e-11 EF-hand domain pair
EF-hand_5 PF13202 478 - 497 6.3e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 62
AccII CGCG 3 cut(s) 1297, 1367, 1610
AccIII TCCGGA 1 cut(s) 1477
AclWI GGATC 6 cut(s) 345, 537, 992, 1005, 1514, 1568
AcoI YGGCCR 1 cut(s) 944
AcsI RAATTY 5 cut(s) 6, 250, 658, 1445, 1574
AcuI CTGAAG 1 cut(s) 804
AcyI GRCGYC 1 cut(s) 865
AfaI GTAC 4 cut(s) 242, 266, 305, 1656
AfeI AGCGCT 1 cut(s) 1424
AfiI CCNNNNNNNGG 3 cut(s) 198, 205, 237
AflII CTTAAG 1 cut(s) 910
AflIII ACRYGT 2 cut(s) 306, 1378
AgsI TTSAA 9 cut(s) 395, 473, 670, 697, 749, 1132, 1244, 1288, 1532
AjiI CACGTC 1 cut(s) 1216
AjnI CCWGG 3 cut(s) 191, 733, 1630
AleI CACNNNNGTG 1 cut(s) 1039
AluBI AGCT 6 cut(s) 184, 430, 611, 1157, 1180, 1436
AluI AGCT 6 cut(s) 184, 430, 611, 1157, 1180, 1436
Alw21I GWGCWC 1 cut(s) 639
Alw26I GTCTC 3 cut(s) 115, 787, 1246
Alw44I GTGCAC 1 cut(s) 635
AlwI GGATC 6 cut(s) 345, 537, 992, 1005, 1514, 1568
AlwNI CAGNNNCTG 1 cut(s) 809
Ama87I CYCGRG 2 cut(s) 1002, 1172
Aor13HI TCCGGA 1 cut(s) 1477
Aor51HI AGCGCT 1 cut(s) 1424
AoxI GGCC 5 cut(s) 207, 436, 699, 806, 944
ApaLI GTGCAC 1 cut(s) 635
ApeKI GCWGC 5 cut(s) 575, 608, 1019, 1417, 1420
ApoI RAATTY 5 cut(s) 6, 250, 658, 1445, 1574
AspLEI GCGC 4 cut(s) 1107, 1261, 1425, 1610
AspS9I GGNCC 4 cut(s) 207, 228, 451, 806
AsuC2I CCSGG 1 cut(s) 106
AsuHPI GGTGA 3 cut(s) 530, 681, 1628
AsuNHI GCTAGC 1 cut(s) 1157
AvaI CYCGRG 2 cut(s) 1002, 1172
AvaII GGWCC 2 cut(s) 228, 451
BaeGI GKGCMC 1 cut(s) 639
BalI TGGCCA 1 cut(s) 946
BamHI GGATCC 1 cut(s) 997
BanI GGYRCC 1 cut(s) 62
Bbv12I GWGCWC 1 cut(s) 639
BbvI GCAGC 5 cut(s) 562, 620, 1006, 1404, 1432
BccI CCATC 3 cut(s) 884, 1457, 1547
BceAI ACGGC 4 cut(s) 5, 76, 194, 540
BciT130I CCWGG 3 cut(s) 193, 735, 1632
BcnI CCSGG 1 cut(s) 106
BcoDI GTCTC 3 cut(s) 115, 787, 1246
BfaI CTAG 2 cut(s) 1158, 1623
BfmI CTRYAG 2 cut(s) 576, 1418
BfoI RGCGCY 2 cut(s) 1262, 1426
BfrI CTTAAG 1 cut(s) 910
BglI GCCNNNNNGGC 1 cut(s) 154
BisI GCNGC 8 cut(s) 69, 146, 576, 609, 1020, 1368, 1418, 1421
BlsI GCNGC 8 cut(s) 70, 147, 577, 610, 1021, 1369, 1419, 1422
Bme1390I CCNGG 4 cut(s) 106, 193, 735, 1632
Bme18I GGWCC 2 cut(s) 228, 451
BmeT110I CYCGRG 2 cut(s) 1002, 1172
BmgBI CACGTC 1 cut(s) 1216
BmgT120I GGNCC 4 cut(s) 207, 228, 451, 806
BmiI GGNNCC 4 cut(s) 64, 197, 208, 999
BmrFI CCNGG 4 cut(s) 106, 193, 735, 1632
BmsI GCATC 6 cut(s) 454, 562, 585, 1073, 1195, 1393
BmtI GCTAGC 1 cut(s) 1161
BoxI GACNNNNGTC 1 cut(s) 799
BpmI CTGGAG 1 cut(s) 175
Bpu10I CCTNAGC 1 cut(s) 1023
BpuEI CTTGAG 2 cut(s) 584, 1325
BpuMI CCSGG 1 cut(s) 106
Bsa29I ATCGAT 1 cut(s) 1080
BsaAI YACGTR 1 cut(s) 309
BsaBI GATNNNNATC 2 cut(s) 1076, 1325
BsaHI GRCGYC 1 cut(s) 865
BsaI GGTCTC 1 cut(s) 115
BsaJI CCNNGG 6 cut(s) 58, 941, 1001, 1040, 1173, 1277
BsaWI WCCGGW 3 cut(s) 84, 1477, 1638
Bsc4I CCNNNNNNNGG 3 cut(s) 198, 205, 237
Bse1I ACTGG 3 cut(s) 1108, 1317, 1504
Bse8I GATNNNNATC 2 cut(s) 1076, 1325
BseAI TCCGGA 1 cut(s) 1477
BseBI CCWGG 3 cut(s) 193, 735, 1632
BseCI ATCGAT 1 cut(s) 1080
BseDI CCNNGG 6 cut(s) 58, 941, 1001, 1040, 1173, 1277
BseJI GATNNNNATC 2 cut(s) 1076, 1325
BseLI CCNNNNNNNGG 3 cut(s) 198, 205, 237
BseMII CTCAG 6 cut(s) 603, 645, 801, 1037, 1347, 1419
BseNI ACTGG 3 cut(s) 1108, 1317, 1504
BseRI GAGGAG 1 cut(s) 1190
BseSI GKGCMC 1 cut(s) 639
BseXI GCAGC 5 cut(s) 562, 620, 1006, 1404, 1432
Bsh1236I CGCG 3 cut(s) 1297, 1367, 1610
Bsh1285I CGRYCG 1 cut(s) 273
BshFI GGCC 5 cut(s) 209, 438, 701, 808, 946
BshNI GGYRCC 1 cut(s) 62
BshVI ATCGAT 1 cut(s) 1080
BsiEI CGRYCG 1 cut(s) 273
BsiHKAI GWGCWC 1 cut(s) 639
BsiHKCI CYCGRG 2 cut(s) 1002, 1172
BsiSI CCGG 5 cut(s) 85, 105, 199, 1478, 1639
BslFI GGGAC 1 cut(s) 1259
BslI CCNNNNNNNGG 3 cut(s) 198, 205, 237
BsmAI GTCTC 3 cut(s) 115, 787, 1246
BsmBI CGTCTC 1 cut(s) 787
BsmFI GGGAC 1 cut(s) 1259
BsmI GAATGC 2 cut(s) 1066, 1497
BsnI GGCC 5 cut(s) 209, 438, 701, 808, 946
Bso31I GGTCTC 1 cut(s) 115
BsoBI CYCGRG 2 cut(s) 1002, 1172
Bsp1286I GDGCHC 1 cut(s) 639
Bsp13I TCCGGA 1 cut(s) 1477
Bsp143I GATC 8 cut(s) 270, 337, 529, 997, 1186, 1195, 1519, 1560
Bsp19I CCATGG 1 cut(s) 941
BspANI GGCC 5 cut(s) 209, 438, 701, 808, 946
BspCNI CTCAG 6 cut(s) 604, 646, 802, 1036, 1348, 1418
BspDI ATCGAT 1 cut(s) 1080
BspEI TCCGGA 1 cut(s) 1477
BspFNI CGCG 3 cut(s) 1297, 1367, 1610
BspLI GGNNCC 4 cut(s) 64, 197, 208, 999
BspMAI CTGCAG 2 cut(s) 580, 1422
BspOI GCTAGC 1 cut(s) 1161
BspPI GGATC 6 cut(s) 345, 537, 992, 1005, 1514, 1568
BspT107I GGYRCC 1 cut(s) 62
BspTI CTTAAG 1 cut(s) 910
BspTNI GGTCTC 1 cut(s) 115
BsrI ACTGG 3 cut(s) 1108, 1317, 1504
BssECI CCNNGG 6 cut(s) 58, 941, 1001, 1040, 1173, 1277
BssMI GATC 8 cut(s) 270, 337, 529, 997, 1186, 1195, 1519, 1560
BssNI GRCGYC 1 cut(s) 865
BssT1I CCWWGG 2 cut(s) 941, 1277
Bst2UI CCWGG 3 cut(s) 193, 735, 1632
Bst4CI ACNGT 3 cut(s) 641, 962, 1137
Bst6I CTCTTC 1 cut(s) 1527
BstACI GRCGYC 1 cut(s) 865
BstAFI CTTAAG 1 cut(s) 910
BstBAI YACGTR 1 cut(s) 309
BstC8I GCNNGC 6 cut(s) 159, 432, 436, 1159, 1263, 1600
BstDSI CCRYGG 3 cut(s) 58, 941, 1040
BstEII GGTNACC 1 cut(s) 1634
BstFNI CGCG 3 cut(s) 1297, 1367, 1610
BstH2I RGCGCY 2 cut(s) 1262, 1426
BstHHI GCGC 4 cut(s) 1107, 1261, 1425, 1610
BstKTI GATC 8 cut(s) 273, 340, 532, 1000, 1189, 1198, 1522, 1563
BstMAI GTCTC 3 cut(s) 115, 787, 1246
BstMBI GATC 8 cut(s) 270, 337, 529, 997, 1186, 1195, 1519, 1560
BstMCI CGRYCG 1 cut(s) 273
BstMWI GCNNNNNNNGC 4 cut(s) 42, 154, 1025, 1148
BstNI CCWGG 3 cut(s) 193, 735, 1632
BstNSI RCATGY 2 cut(s) 1105, 1382
BstPAI GACNNNNGTC 1 cut(s) 799
BstPI GGTNACC 1 cut(s) 1634
BstSCI CCNGG 4 cut(s) 104, 191, 733, 1630
BstSFI CTRYAG 2 cut(s) 576, 1418
BstSLI GKGCMC 1 cut(s) 639
BstUI CGCG 3 cut(s) 1297, 1367, 1610
BstV1I GCAGC 5 cut(s) 562, 620, 1006, 1404, 1432
BstX2I RGATCY 1 cut(s) 997
BstYI RGATCY 1 cut(s) 997
Bsu15I ATCGAT 1 cut(s) 1080
BsuRI GGCC 5 cut(s) 209, 438, 701, 808, 946
BsuTUI ATCGAT 1 cut(s) 1080
BtgI CCRYGG 3 cut(s) 58, 941, 1040
BtrI CACGTC 1 cut(s) 1216
BtsI GCAGTG 1 cut(s) 769
BtsIMutI CAGTG 6 cut(s) 37, 561, 769, 967, 1353, 1497
Cac8I GCNNGC 6 cut(s) 159, 432, 436, 1159, 1263, 1600
CaiI CAGNNNCTG 1 cut(s) 809
CfoI GCGC 4 cut(s) 1107, 1261, 1425, 1610
Cfr13I GGNCC 4 cut(s) 207, 228, 451, 806
ClaI ATCGAT 1 cut(s) 1080
CpoI CGGWCCG 1 cut(s) 228
CseI GACGC 2 cut(s) 804, 854
CsiI ACCWGGT 1 cut(s) 1630
Csp6I GTAC 4 cut(s) 241, 265, 304, 1655
CspCI CAANNNNNGTGG 6 cut(s) 13, 48, 691, 726, 1197, 1232
CspI CGGWCCG 1 cut(s) 228
CviAII CATG 8 cut(s) 290, 440, 626, 646, 942, 1102, 1379, 1400
CviQI GTAC 4 cut(s) 241, 265, 304, 1655
DpnI GATC 8 cut(s) 272, 339, 531, 999, 1188, 1197, 1521, 1562
DpnII GATC 8 cut(s) 270, 337, 529, 997, 1186, 1195, 1519, 1560
EaeI YGGCCR 1 cut(s) 944
Eam1104I CTCTTC 1 cut(s) 1527
EarI CTCTTC 1 cut(s) 1527
EciI GGCGGA 2 cut(s) 7, 1553
Eco130I CCWWGG 2 cut(s) 941, 1277
Eco31I GGTCTC 1 cut(s) 115
Eco47I GGWCC 2 cut(s) 228, 451
Eco47III AGCGCT 1 cut(s) 1424
Eco57I CTGAAG 1 cut(s) 804
Eco88I CYCGRG 2 cut(s) 1002, 1172
Eco91I GGTNACC 1 cut(s) 1634
EcoO109I RGGNCCY 1 cut(s) 806
EcoO65I GGTNACC 1 cut(s) 1634
EcoRI GAATTC 1 cut(s) 1574
EcoRII CCWGG 3 cut(s) 191, 733, 1630
EcoT14I CCWWGG 2 cut(s) 941, 1277
EcoT22I ATGCAT 2 cut(s) 469, 1066
ErhI CCWWGG 2 cut(s) 941, 1277
Esp3I CGTCTC 1 cut(s) 787
FaeI CATG 8 cut(s) 293, 443, 629, 649, 945, 1105, 1382, 1403
FalI AAGNNNNNCTT 2 cut(s) 893, 925
FaqI GGGAC 1 cut(s) 1259
FatI CATG 8 cut(s) 289, 439, 625, 645, 941, 1101, 1378, 1399
Fnu4HI GCNGC 8 cut(s) 69, 146, 576, 609, 1020, 1368, 1418, 1421
Fsp4HI GCNGC 8 cut(s) 69, 146, 576, 609, 1020, 1368, 1418, 1421
FspBI CTAG 2 cut(s) 1158, 1623
GlaI GCGC 4 cut(s) 1106, 1260, 1424, 1609
GluI GCNGC 8 cut(s) 69, 146, 576, 609, 1020, 1368, 1418, 1421
GsuI CTGGAG 1 cut(s) 175
HaeII RGCGCY 2 cut(s) 1262, 1426
HaeIII GGCC 5 cut(s) 209, 438, 701, 808, 946
HapII CCGG 5 cut(s) 85, 105, 199, 1478, 1639
HgaI GACGC 2 cut(s) 804, 854
HhaI GCGC 4 cut(s) 1107, 1261, 1425, 1610
Hin1I GRCGYC 1 cut(s) 865
Hin1II CATG 8 cut(s) 293, 443, 629, 649, 945, 1105, 1382, 1403
Hin6I GCGC 4 cut(s) 1105, 1259, 1423, 1608
HinP1I GCGC 4 cut(s) 1105, 1259, 1423, 1608
HinfI GANTC 7 cut(s) 376, 479, 686, 812, 1072, 1292, 1403
HpaII CCGG 5 cut(s) 85, 105, 199, 1478, 1639
HphI GGTGA 3 cut(s) 530, 681, 1628
Hpy166II GTNNAC 4 cut(s) 267, 306, 637, 1348
Hpy188I TCNGA 7 cut(s) 534, 721, 817, 1069, 1186, 1408, 1573
Hpy8I GTNNAC 4 cut(s) 267, 306, 637, 1348
Hpy99I CGWCG 2 cut(s) 936, 1340
HpyAV CCTTC 7 cut(s) 196, 506, 541, 691, 988, 1046, 1645
HpyCH4III ACNGT 3 cut(s) 641, 962, 1137
HpyCH4IV ACGT 2 cut(s) 308, 1215
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 154, 1025, 1148
HpySE526I ACGT 2 cut(s) 308, 1215
Hsp92I GRCGYC 1 cut(s) 865
Hsp92II CATG 8 cut(s) 293, 443, 629, 649, 945, 1105, 1382, 1403
HspAI GCGC 4 cut(s) 1105, 1259, 1423, 1608
Kpn2I TCCGGA 1 cut(s) 1477
Kzo9I GATC 8 cut(s) 270, 337, 529, 997, 1186, 1195, 1519, 1560
LmnI GCTCC 4 cut(s) 195, 427, 784, 1177
Lsp1109I GCAGC 5 cut(s) 562, 620, 1006, 1404, 1432
LweI GCATC 6 cut(s) 454, 562, 585, 1073, 1195, 1393
MabI ACCWGGT 1 cut(s) 1630
MaeI CTAG 2 cut(s) 1158, 1623
MaeII ACGT 2 cut(s) 308, 1215
MaeIII GTNAC 6 cut(s) 557, 641, 956, 984, 1618, 1634
MalI GATC 8 cut(s) 272, 339, 531, 999, 1188, 1197, 1521, 1562
MboI GATC 8 cut(s) 270, 337, 529, 997, 1186, 1195, 1519, 1560
MboII GAAGA 6 cut(s) 407, 485, 556, 899, 1256, 1544
MfeI CAATTG 2 cut(s) 1209, 1388
MflI RGATCY 1 cut(s) 997
MhlI GDGCHC 1 cut(s) 639
MlsI TGGCCA 1 cut(s) 946
MluNI TGGCCA 1 cut(s) 946
MlyI GAGTC 3 cut(s) 680, 821, 1397
Mox20I TGGCCA 1 cut(s) 946
Mph1103I ATGCAT 2 cut(s) 469, 1066
MroI TCCGGA 1 cut(s) 1477
MscI TGGCCA 1 cut(s) 946
MseI TTAA 5 cut(s) 402, 900, 911, 978, 1167
MslI CAYNNNNRTG 5 cut(s) 444, 459, 624, 1039, 1641
Msp20I TGGCCA 1 cut(s) 946
MspA1I CMGCKG 1 cut(s) 611
MspCI CTTAAG 1 cut(s) 910
MspI CCGG 5 cut(s) 85, 105, 199, 1478, 1639
MspR9I CCNGG 4 cut(s) 106, 193, 735, 1632
MunI CAATTG 2 cut(s) 1209, 1388
Mva1269I GAATGC 2 cut(s) 1066, 1497
MvaI CCWGG 3 cut(s) 193, 735, 1632
MvnI CGCG 3 cut(s) 1297, 1367, 1610
MwoI GCNNNNNNNGC 4 cut(s) 42, 154, 1025, 1148
NciI CCSGG 1 cut(s) 106
NcoI CCATGG 1 cut(s) 941
NdeII GATC 8 cut(s) 270, 337, 529, 997, 1186, 1195, 1519, 1560
NheI GCTAGC 1 cut(s) 1157
NlaIII CATG 8 cut(s) 293, 443, 629, 649, 945, 1105, 1382, 1403
NlaIV GGNNCC 4 cut(s) 64, 197, 208, 999
NmuCI GTSAC 3 cut(s) 641, 1618, 1634
NsiI ATGCAT 2 cut(s) 469, 1066
NspI RCATGY 2 cut(s) 1105, 1382
OliI CACNNNNGTG 1 cut(s) 1039
PciI ACATGT 1 cut(s) 1378
PcsI WCGNNNNNNNCGW 1 cut(s) 1086
PctI GAATGC 2 cut(s) 1066, 1497
PfeI GAWTC 4 cut(s) 376, 479, 1072, 1292
PfoI TCCNGGA 1 cut(s) 191
PkrI GCNGC 8 cut(s) 70, 147, 577, 610, 1021, 1369, 1419, 1422
Ple19I CGATCG 1 cut(s) 273
PleI GAGTC 3 cut(s) 680, 820, 1397
PpsI GAGTC 3 cut(s) 680, 820, 1397
Ppu21I YACGTR 1 cut(s) 309
PscI ACATGT 1 cut(s) 1378
PshAI GACNNNNGTC 1 cut(s) 799
Psp6I CCWGG 3 cut(s) 191, 733, 1630
PspEI GGTNACC 1 cut(s) 1634
PspGI CCWGG 3 cut(s) 191, 733, 1630
PspN4I GGNNCC 4 cut(s) 64, 197, 208, 999
PspPI GGNCC 4 cut(s) 207, 228, 451, 806
PstI CTGCAG 2 cut(s) 580, 1422
PstNI CAGNNNCTG 1 cut(s) 809
PsuI RGATCY 1 cut(s) 997
PvuI CGATCG 1 cut(s) 273
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 4 cut(s) 242, 266, 305, 1656
RsaNI GTAC 4 cut(s) 241, 265, 304, 1655
RseI CAYNNNNRTG 5 cut(s) 444, 459, 624, 1039, 1641
Rsr2I CGGWCCG 1 cut(s) 228
RsrII CGGWCCG 1 cut(s) 228
SaqAI TTAA 5 cut(s) 402, 900, 911, 978, 1167
SatI GCNGC 8 cut(s) 69, 146, 576, 609, 1020, 1368, 1418, 1421
Sau3AI GATC 8 cut(s) 270, 337, 529, 997, 1186, 1195, 1519, 1560
Sau96I GGNCC 4 cut(s) 207, 228, 451, 806
SchI GAGTC 3 cut(s) 680, 821, 1397
ScrFI CCNGG 4 cut(s) 106, 193, 735, 1632
SduI GDGCHC 1 cut(s) 639
SexAI ACCWGGT 1 cut(s) 1630
SfaNI GCATC 6 cut(s) 454, 562, 585, 1073, 1195, 1393
SfcI CTRYAG 2 cut(s) 576, 1418
SinI GGWCC 2 cut(s) 228, 451
SmiMI CAYNNNNRTG 5 cut(s) 444, 459, 624, 1039, 1641
SmlI CTYRAG 3 cut(s) 599, 910, 1304
SmoI CTYRAG 3 cut(s) 599, 910, 1304
SspMI CTAG 2 cut(s) 1158, 1623
StyD4I CCNGG 4 cut(s) 104, 191, 733, 1630
StyI CCWWGG 2 cut(s) 941, 1277
TaaI ACNGT 3 cut(s) 641, 962, 1137
TaiI ACGT 2 cut(s) 311, 1218
TaqI TCGA 5 cut(s) 255, 340, 1080, 1449, 1545
TaqII GACCGA 2 cut(s) 245, 899
TauI GCSGC 3 cut(s) 71, 148, 1370
TfiI GAWTC 4 cut(s) 376, 479, 1072, 1292
Tru1I TTAA 5 cut(s) 402, 900, 911, 978, 1167
Tru9I TTAA 5 cut(s) 402, 900, 911, 978, 1167
TscAI CASTG 6 cut(s) 37, 568, 769, 967, 1360, 1504
TseFI GTSAC 3 cut(s) 641, 1618, 1634
TseI GCWGC 5 cut(s) 575, 608, 1019, 1417, 1420
Tsp45I GTSAC 3 cut(s) 641, 1618, 1634
TspDTI ATGAA 5 cut(s) 155, 492, 662, 715, 1459
TspGWI ACGGA 2 cut(s) 1078, 1444
TspRI CASTG 6 cut(s) 37, 568, 769, 967, 1360, 1504
Vha464I CTTAAG 1 cut(s) 910
VneI GTGCAC 1 cut(s) 635
VpaK11BI GGWCC 2 cut(s) 228, 451
XapI RAATTY 5 cut(s) 6, 250, 658, 1445, 1574
XceI RCATGY 2 cut(s) 1105, 1382
XspI CTAG 2 cut(s) 1158, 1623
Zsp2I ATGCAT 2 cut(s) 469, 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.