MD15G1245400.v1.1

Calcium-dependent protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006979
Physical Location & Seq
Reverse (-)
20223123 .. 20227605
4483 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1245400.v1.1.491

Sequence Viewer

Length: 1659 bp
ATGGGAGGGTGCTTCTCCACCATCAAAGTCAGTGGCTCAAATAGCAACAACACTGCCCCCGCCACCCCTGCCGACAATCACAACCGAAAACAGAGCACAACAGCCGGGAAGCAAGAACAGAGACCCACCCACGGACCCAATGAACCGCCCAAGAAGCAGGTTGTTCATAAACCCAAAAACAAGCTCAACTCCAGGAGCCGGACGGGGGTCATTCCCTGCGGAAAGCGGACCGATTTCGGGTACGATAAGAATTTCGATAAGCGGTACACGATCGGGAAGTTGTTGGGTCACGGACAATTTGGGTACACGTACGTTGCAACCGATAGGGCAAATGGGGATCGAGTTGCGGTCAAGAGGATTGATAAGAACAAGATGATTCTACCTATTGCTGTTGAGGATGTTAAGCGAGAGGTCAGGATATTACAGGCCCTTGCAGGCCATGAAAATGTGGTCCACTTTTATAATGCCTGTGAAGATGACTCTTATGTGTATATAGTTATGGAGTTATGTGAAGGCGGTGAGTTGCTGGATCGGATTCTAGAGAAGAAGGACAGCCGTTACACTGAAAAGGATGCCGCAGTAGTTGTAAGGCAGATGCTCAAGGTTGCAGCTGAGTGTCATTTACATGGTCTGGTGCACCGTGACATGAAACCTGAGAATTTCCTTTTCAAGTCGAAAGCCTTAGACTCGCCTTTGAAGGCAACAGACTTTGGTTTGTCTGACTTCATAAAACCAGGGAAGAAGTTTCAAGATATTGTTGGCAGTGCTTACTACGTTGCTCCTGAAGTATTGAAACGCAAGTCAGGGCCTGAGTCAGATGTTTGGAGTATTGGTGTTATTACCTACATTTTGCTTTGCGGGAGACGTCCATTTTGGGATAAGACCGAAGATGGTATATTTAAGGAGGTCTTAAGGAAGAAGCCTGATTTTGGTCGCAAACCATGGCCAAGCATAAGTAACAGTGCTAAAGATTTTGTTAAGAAGTTACTTGTGAAGGATCCTCGGGCGAGGCTAACTGCTGCTCAGGCTCTATCACACCCATGGGTTAAAGAAGGAGGTGATGCATCAGAGATTCCCATCGATATATCTGTTCTCAATAACATGCGCCAGTTCGTGAGATATAGTCGTTTGAAACAGTTTGCTCTAAGGGCATTAGCTAGCACGCTTAATCCTGAGGAGCTATCTGATCTCAAAGATCAATTTGATGCAATTGACATGGATAAGAATGGTGCGATTAGTCTCGAAGAAATGAGACAGGCACTTGCTAAAGATGTCCCTTGGAAATTGAAAGATTCGCGTGTCCTTGAGATACTGGAAGCGATTGACAGCAACACAGACGGGCTTGTGGACTTCACTGAGTTTGTCGCTGCTACTCTACATGTGCATCAATTGGAGGAACATGACTCACAGAAATGGCAGCAGCGCTCACGGGCAGCTTTTGAGAAATTCGACATAGATAGAGATGGGTTTATAACTCCAGAGGAACTTAGAATGCACACGGGTTTAAAAGGTTCTATAGATCCACTTCTTGACGAGGCGGACATTGACAAAGATGGGAGGATCAGCCTATCAGAATTCCGTAGACTTCTAAGAACTGCGAGCATTACCTCAAGAACTGTGACTAGCTCACCTGGTCACCGGAATGCTAGGAAGCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002831 GO:0002832 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0004721 GO:0004722 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016788 GO:0016791 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022603 GO:0023052 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043900 GO:0043901 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0048519 GO:0048583 GO:0048585 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060688 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080134 GO:0097305 GO:0097306 GO:0098542 GO:0140096 GO:1900424 GO:1900425 GO:1900618 GO:1901564 GO:1901700 GO:1901701 GO:1905428 GO:2000026 GO:2000032
KEGG Pathways
Metabolic & Signaling
ko04626 ko05145 map04626 map05145
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 Pkinase

Protein Analysis

553

Amino Acids

62.29

Weight (kDa)

9.08

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 89 - 349 4.1e-70 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 91 - 341 1.4e-29 Protein tyrosine and serine/threonine kinase
Kinase-like PF14531 175 - 299 8.6e-06 Kinase-like
EF-hand_7 PF13499 395 - 457 4.8e-11 EF-hand domain pair
EF-hand_5 PF13202 397 - 419 5.5e-06 EF hand
EF-hand_7 PF13499 476 - 530 1.2e-10 EF-hand domain pair
EF-hand_5 PF13202 478 - 497 3.9e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 462, 1472
AatII GACGTC 1 cut(s) 868
Acc36I ACCTGC 1 cut(s) 148
AccI GTMKAC 1 cut(s) 1582
AccII CGCG 1 cut(s) 1297
AclWI GGATC 6 cut(s) 345, 537, 992, 1005, 1514, 1568
AcoI YGGCCR 1 cut(s) 944
AcsI RAATTY 4 cut(s) 250, 658, 1445, 1574
AcuI CTGAAG 1 cut(s) 804
AcyI GRCGYC 1 cut(s) 865
AfaI GTAC 4 cut(s) 242, 266, 305, 311
AfeI AGCGCT 1 cut(s) 1424
AfiI CCNNNNNNNGG 5 cut(s) 131, 198, 205, 237, 929
AflII CTTAAG 1 cut(s) 910
AflIII ACRYGT 2 cut(s) 306, 1378
AgsI TTSAA 6 cut(s) 670, 697, 749, 793, 1132, 1288
AjnI CCWGG 3 cut(s) 191, 733, 1630
AluBI AGCT 7 cut(s) 184, 611, 1157, 1180, 1436, 1626, 1654
AluI AGCT 7 cut(s) 184, 611, 1157, 1180, 1436, 1626, 1654
Alw21I GWGCWC 2 cut(s) 98, 639
Alw26I GTCTC 4 cut(s) 115, 856, 1244, 1246
Alw44I GTGCAC 1 cut(s) 635
AlwI GGATC 6 cut(s) 345, 537, 992, 1005, 1514, 1568
AlwNI CAGNNNCTG 1 cut(s) 809
Ama87I CYCGRG 1 cut(s) 1002
Aor51HI AGCGCT 1 cut(s) 1424
AoxI GGCC 4 cut(s) 426, 436, 806, 944
ApaLI GTGCAC 1 cut(s) 635
ApeKI GCWGC 6 cut(s) 608, 1019, 1367, 1417, 1420, 1433
ApoI RAATTY 4 cut(s) 250, 658, 1445, 1574
ArsI GACNNNNNNTTYG 2 cut(s) 855, 887
AspLEI GCGC 2 cut(s) 1107, 1425
AspS9I GGNCC 5 cut(s) 134, 228, 427, 451, 806
AsuC2I CCSGG 1 cut(s) 106
AsuHPI GGTGA 4 cut(s) 530, 1070, 1620, 1628
AsuNHI GCTAGC 1 cut(s) 1157
AvaI CYCGRG 1 cut(s) 1002
AvaII GGWCC 3 cut(s) 134, 228, 451
AxyI CCTNAGG 1 cut(s) 1173
BaeGI GKGCMC 1 cut(s) 639
BalI TGGCCA 1 cut(s) 946
BamHI GGATCC 1 cut(s) 997
Bbv12I GWGCWC 2 cut(s) 98, 639
BbvI GCAGC 6 cut(s) 620, 1006, 1354, 1429, 1432, 1445
BccI CCATC 5 cut(s) 29, 884, 1085, 1457, 1547
BceAI ACGGC 1 cut(s) 540
BciT130I CCWGG 3 cut(s) 193, 735, 1632
BcnI CCSGG 1 cut(s) 106
BcoDI GTCTC 4 cut(s) 115, 856, 1244, 1246
BfaI CTAG 4 cut(s) 539, 1158, 1623, 1647
BfmI CTRYAG 2 cut(s) 1515, 1655
BfoI RGCGCY 1 cut(s) 1426
BfrI CTTAAG 1 cut(s) 910
BfuAI ACCTGC 1 cut(s) 148
BisI GCNGC 7 cut(s) 576, 609, 1020, 1368, 1418, 1421, 1434
BlsI GCNGC 7 cut(s) 577, 610, 1021, 1369, 1419, 1422, 1435
Bme1390I CCNGG 4 cut(s) 106, 193, 735, 1632
Bme18I GGWCC 3 cut(s) 134, 228, 451
BmeT110I CYCGRG 1 cut(s) 1002
BmgT120I GGNCC 5 cut(s) 134, 228, 427, 451, 806
BmiI GGNNCC 3 cut(s) 136, 197, 999
BmrFI CCNGG 4 cut(s) 106, 193, 735, 1632
BmsI GCATC 6 cut(s) 562, 585, 1051, 1073, 1195, 1393
BmtI GCTAGC 1 cut(s) 1161
BoxI GACNNNNGTC 1 cut(s) 206
BpmI CTGGAG 2 cut(s) 175, 1461
Bpu10I CCTNAGC 1 cut(s) 1023
BpuEI CTTGAG 3 cut(s) 584, 1325, 1594
BpuMI CCSGG 1 cut(s) 106
Bsa29I ATCGAT 1 cut(s) 1080
BsaAI YACGTR 1 cut(s) 309
BsaBI GATNNNNATC 1 cut(s) 1076
BsaHI GRCGYC 1 cut(s) 865
BsaI GGTCTC 1 cut(s) 115
BsaJI CCNNGG 6 cut(s) 130, 734, 941, 1001, 1040, 1277
BsaWI WCCGGW 1 cut(s) 1638
BsaXI ACNNNNNCTCC 2 cut(s) 817, 847
Bsc4I CCNNNNNNNGG 5 cut(s) 131, 198, 205, 237, 929
Bse1I ACTGG 2 cut(s) 1108, 1317
Bse21I CCTNAGG 1 cut(s) 1173
Bse8I GATNNNNATC 1 cut(s) 1076
BseBI CCWGG 3 cut(s) 193, 735, 1632
BseCI ATCGAT 1 cut(s) 1080
BseDI CCNNGG 6 cut(s) 130, 734, 941, 1001, 1040, 1277
BseGI GGATG 2 cut(s) 403, 577
BseJI GATNNNNATC 1 cut(s) 1076
BseLI CCNNNNNNNGG 5 cut(s) 131, 198, 205, 237, 929
BseMII CTCAG 6 cut(s) 603, 645, 801, 1037, 1164, 1347
BseNI ACTGG 2 cut(s) 1108, 1317
BseRI GAGGAG 1 cut(s) 1190
BseSI GKGCMC 1 cut(s) 639
BseXI GCAGC 6 cut(s) 620, 1006, 1354, 1429, 1432, 1445
Bsh1236I CGCG 1 cut(s) 1297
Bsh1285I CGRYCG 1 cut(s) 273
BshFI GGCC 4 cut(s) 428, 438, 808, 946
BshVI ATCGAT 1 cut(s) 1080
BsiEI CGRYCG 1 cut(s) 273
BsiHKAI GWGCWC 2 cut(s) 98, 639
BsiHKCI CYCGRG 1 cut(s) 1002
BsiSI CCGG 3 cut(s) 105, 199, 1639
BsiWI CGTACG 1 cut(s) 309
BslFI GGGAC 1 cut(s) 1259
BslI CCNNNNNNNGG 5 cut(s) 131, 198, 205, 237, 929
BsmAI GTCTC 4 cut(s) 115, 856, 1244, 1246
BsmBI CGTCTC 1 cut(s) 856
BsmFI GGGAC 1 cut(s) 1259
BsmI GAATGC 2 cut(s) 1497, 1648
BsnI GGCC 4 cut(s) 428, 438, 808, 946
Bso31I GGTCTC 1 cut(s) 115
BsoBI CYCGRG 1 cut(s) 1002
Bsp1286I GDGCHC 2 cut(s) 98, 639
Bsp143I GATC 8 cut(s) 270, 337, 529, 997, 1186, 1195, 1519, 1560
Bsp19I CCATGG 2 cut(s) 941, 1040
BspANI GGCC 4 cut(s) 428, 438, 808, 946
BspCNI CTCAG 6 cut(s) 604, 646, 802, 1036, 1165, 1348
BspDI ATCGAT 1 cut(s) 1080
BspFNI CGCG 1 cut(s) 1297
BspLI GGNNCC 3 cut(s) 136, 197, 999
BspMI ACCTGC 1 cut(s) 148
BspOI GCTAGC 1 cut(s) 1161
BspPI GGATC 6 cut(s) 345, 537, 992, 1005, 1514, 1568
BspTI CTTAAG 1 cut(s) 910
BspTNI GGTCTC 1 cut(s) 115
BsrI ACTGG 2 cut(s) 1108, 1317
BssECI CCNNGG 6 cut(s) 130, 734, 941, 1001, 1040, 1277
BssMI GATC 8 cut(s) 270, 337, 529, 997, 1186, 1195, 1519, 1560
BssNI GRCGYC 1 cut(s) 865
BssT1I CCWWGG 3 cut(s) 941, 1040, 1277
Bst2UI CCWGG 3 cut(s) 193, 735, 1632
Bst4CI ACNGT 4 cut(s) 641, 962, 1137, 1618
BstACI GRCGYC 1 cut(s) 865
BstAFI CTTAAG 1 cut(s) 910
BstBAI YACGTR 1 cut(s) 309
BstC8I GCNNGC 4 cut(s) 436, 1159, 1163, 1600
BstDSI CCRYGG 3 cut(s) 130, 941, 1040
BstEII GGTNACC 1 cut(s) 1634
BstF5I GGATG 2 cut(s) 403, 577
BstFNI CGCG 1 cut(s) 1297
BstH2I RGCGCY 1 cut(s) 1426
BstHHI GCGC 2 cut(s) 1107, 1425
BstKTI GATC 8 cut(s) 273, 340, 532, 1000, 1189, 1198, 1522, 1563
BstMAI GTCTC 4 cut(s) 115, 856, 1244, 1246
BstMBI GATC 8 cut(s) 270, 337, 529, 997, 1186, 1195, 1519, 1560
BstMCI CGRYCG 1 cut(s) 273
BstMWI GCNNNNNNNGC 5 cut(s) 42, 68, 154, 1025, 1148
BstNI CCWGG 3 cut(s) 193, 735, 1632
BstNSI RCATGY 2 cut(s) 1105, 1382
BstPAI GACNNNNGTC 1 cut(s) 206
BstPI GGTNACC 1 cut(s) 1634
BstSCI CCNGG 4 cut(s) 104, 191, 733, 1630
BstSFI CTRYAG 2 cut(s) 1515, 1655
BstSLI GKGCMC 1 cut(s) 639
BstUI CGCG 1 cut(s) 1297
BstV1I GCAGC 6 cut(s) 620, 1006, 1354, 1429, 1432, 1445
BstX2I RGATCY 2 cut(s) 997, 1519
BstYI RGATCY 2 cut(s) 997, 1519
Bsu15I ATCGAT 1 cut(s) 1080
Bsu36I CCTNAGG 1 cut(s) 1173
BsuRI GGCC 4 cut(s) 428, 438, 808, 946
BsuTUI ATCGAT 1 cut(s) 1080
BtgI CCRYGG 3 cut(s) 130, 941, 1040
BtsCI GGATG 2 cut(s) 403, 577
BtsI GCAGTG 2 cut(s) 51, 769
BtsIMutI CAGTG 6 cut(s) 37, 51, 561, 769, 967, 1353
BveI ACCTGC 1 cut(s) 148
Cac8I GCNNGC 4 cut(s) 436, 1159, 1163, 1600
CaiI CAGNNNCTG 1 cut(s) 809
CfoI GCGC 2 cut(s) 1107, 1425
Cfr13I GGNCC 5 cut(s) 134, 228, 427, 451, 806
ClaI ATCGAT 1 cut(s) 1080
CpoI CGGWCCG 1 cut(s) 228
CsiI ACCWGGT 1 cut(s) 1630
Csp6I GTAC 4 cut(s) 241, 265, 304, 310
CspCI CAANNNNNGTGG 2 cut(s) 13, 48
CspI CGGWCCG 1 cut(s) 228
CviAII CATG 9 cut(s) 440, 626, 646, 942, 1041, 1102, 1216, 1379, 1400
CviQI GTAC 4 cut(s) 241, 265, 304, 310
DpnI GATC 8 cut(s) 272, 339, 531, 999, 1188, 1197, 1521, 1562
DpnII GATC 8 cut(s) 270, 337, 529, 997, 1186, 1195, 1519, 1560
DraI TTTAAA 1 cut(s) 1506
EaeI YGGCCR 1 cut(s) 944
EciI GGCGGA 1 cut(s) 1553
Eco130I CCWWGG 3 cut(s) 941, 1040, 1277
Eco31I GGTCTC 1 cut(s) 115
Eco47I GGWCC 3 cut(s) 134, 228, 451
Eco47III AGCGCT 1 cut(s) 1424
Eco57I CTGAAG 1 cut(s) 804
Eco81I CCTNAGG 1 cut(s) 1173
Eco88I CYCGRG 1 cut(s) 1002
Eco91I GGTNACC 1 cut(s) 1634
EcoO109I RGGNCCY 2 cut(s) 427, 806
EcoO65I GGTNACC 1 cut(s) 1634
EcoRI GAATTC 1 cut(s) 1574
EcoRII CCWGG 3 cut(s) 191, 733, 1630
EcoT14I CCWWGG 3 cut(s) 941, 1040, 1277
EcoT22I ATGCAT 1 cut(s) 1066
ErhI CCWWGG 3 cut(s) 941, 1040, 1277
Esp3I CGTCTC 1 cut(s) 856
FaeI CATG 9 cut(s) 443, 629, 649, 945, 1044, 1105, 1219, 1382, 1403
FalI AAGNNNNNCTT 2 cut(s) 893, 925
FaqI GGGAC 1 cut(s) 1259
FatI CATG 9 cut(s) 439, 625, 645, 941, 1040, 1101, 1215, 1378, 1399
FauI CCCGC 2 cut(s) 67, 851
FblI GTMKAC 1 cut(s) 1582
Fnu4HI GCNGC 7 cut(s) 576, 609, 1020, 1368, 1418, 1421, 1434
FokI GGATG 2 cut(s) 410, 584
Fsp4HI GCNGC 7 cut(s) 576, 609, 1020, 1368, 1418, 1421, 1434
FspBI CTAG 4 cut(s) 539, 1158, 1623, 1647
GlaI GCGC 2 cut(s) 1106, 1424
GluI GCNGC 7 cut(s) 576, 609, 1020, 1368, 1418, 1421, 1434
GsuI CTGGAG 2 cut(s) 175, 1461
HaeII RGCGCY 1 cut(s) 1426
HaeIII GGCC 4 cut(s) 428, 438, 808, 946
HapII CCGG 3 cut(s) 105, 199, 1639
HhaI GCGC 2 cut(s) 1107, 1425
Hin1I GRCGYC 1 cut(s) 865
Hin1II CATG 9 cut(s) 443, 629, 649, 945, 1044, 1105, 1219, 1382, 1403
Hin6I GCGC 2 cut(s) 1105, 1423
HinP1I GCGC 2 cut(s) 1105, 1423
HinfI GANTC 8 cut(s) 376, 479, 535, 686, 812, 1072, 1292, 1403
HpaII CCGG 3 cut(s) 105, 199, 1639
HphI GGTGA 4 cut(s) 530, 1070, 1620, 1628
Hpy166II GTNNAC 6 cut(s) 267, 306, 454, 637, 1348, 1583
Hpy188I TCNGA 6 cut(s) 534, 721, 817, 1069, 1186, 1573
Hpy8I GTNNAC 6 cut(s) 267, 306, 454, 637, 1348, 1583
HpyAV CCTTC 5 cut(s) 506, 541, 691, 988, 1046
HpyCH4III ACNGT 4 cut(s) 641, 962, 1137, 1618
HpyCH4IV ACGT 4 cut(s) 308, 312, 774, 865
HpyCH4V TGCA 8 cut(s) 317, 434, 608, 637, 1064, 1208, 1384, 1495
HpyF10VI GCNNNNNNNGC 5 cut(s) 42, 68, 154, 1025, 1148
HpySE526I ACGT 4 cut(s) 308, 312, 774, 865
Hsp92I GRCGYC 1 cut(s) 865
Hsp92II CATG 9 cut(s) 443, 629, 649, 945, 1044, 1105, 1219, 1382, 1403
HspAI GCGC 2 cut(s) 1105, 1423
Kzo9I GATC 8 cut(s) 270, 337, 529, 997, 1186, 1195, 1519, 1560
LmnI GCTCC 3 cut(s) 195, 784, 1177
Lsp1109I GCAGC 6 cut(s) 620, 1006, 1354, 1429, 1432, 1445
LweI GCATC 6 cut(s) 562, 585, 1051, 1073, 1195, 1393
MabI ACCWGGT 1 cut(s) 1630
MaeI CTAG 4 cut(s) 539, 1158, 1623, 1647
MaeII ACGT 4 cut(s) 308, 312, 774, 865
MaeIII GTNAC 7 cut(s) 287, 557, 641, 956, 984, 1618, 1634
MalI GATC 8 cut(s) 272, 339, 531, 999, 1188, 1197, 1521, 1562
MboI GATC 8 cut(s) 270, 337, 529, 997, 1186, 1195, 1519, 1560
MboII GAAGA 6 cut(s) 485, 556, 751, 899, 928, 1256
MfeI CAATTG 2 cut(s) 1209, 1388
MflI RGATCY 2 cut(s) 997, 1519
MhlI GDGCHC 2 cut(s) 98, 639
MlsI TGGCCA 1 cut(s) 946
MluCI AATT 9 cut(s) 250, 296, 658, 1199, 1209, 1283, 1388, 1445, 1574
MluNI TGGCCA 1 cut(s) 946
MlyI GAGTC 4 cut(s) 473, 680, 821, 1397
Mox20I TGGCCA 1 cut(s) 946
Mph1103I ATGCAT 1 cut(s) 1066
MscI TGGCCA 1 cut(s) 946
MseI TTAA 7 cut(s) 402, 900, 911, 978, 1047, 1167, 1505
MslI CAYNNNNRTG 5 cut(s) 444, 624, 1039, 1411, 1641
Msp20I TGGCCA 1 cut(s) 946
MspA1I CMGCKG 1 cut(s) 611
MspCI CTTAAG 1 cut(s) 910
MspI CCGG 3 cut(s) 105, 199, 1639
MspR9I CCNGG 4 cut(s) 106, 193, 735, 1632
MunI CAATTG 2 cut(s) 1209, 1388
Mva1269I GAATGC 2 cut(s) 1497, 1648
MvaI CCWGG 3 cut(s) 193, 735, 1632
MvnI CGCG 1 cut(s) 1297
MwoI GCNNNNNNNGC 5 cut(s) 42, 68, 154, 1025, 1148
NciI CCSGG 1 cut(s) 106
NcoI CCATGG 2 cut(s) 941, 1040
NdeII GATC 8 cut(s) 270, 337, 529, 997, 1186, 1195, 1519, 1560
NheI GCTAGC 1 cut(s) 1157
NlaIII CATG 9 cut(s) 443, 629, 649, 945, 1044, 1105, 1219, 1382, 1403
NlaIV GGNNCC 3 cut(s) 136, 197, 999
NmuCI GTSAC 4 cut(s) 287, 641, 1618, 1634
NsiI ATGCAT 1 cut(s) 1066
NspI RCATGY 2 cut(s) 1105, 1382
PciI ACATGT 1 cut(s) 1378
PcsI WCGNNNNNNNCGW 1 cut(s) 318
PctI GAATGC 2 cut(s) 1497, 1648
PfeI GAWTC 4 cut(s) 376, 535, 1072, 1292
Pfl23II CGTACG 1 cut(s) 309
PfoI TCCNGGA 1 cut(s) 191
PkrI GCNGC 7 cut(s) 577, 610, 1021, 1369, 1419, 1422, 1435
Ple19I CGATCG 1 cut(s) 273
PleI GAGTC 4 cut(s) 473, 680, 820, 1397
PpsI GAGTC 4 cut(s) 473, 680, 820, 1397
Ppu21I YACGTR 1 cut(s) 309
PscI ACATGT 1 cut(s) 1378
PshAI GACNNNNGTC 1 cut(s) 206
PsiI TTATAA 2 cut(s) 462, 1472
Psp6I CCWGG 3 cut(s) 191, 733, 1630
PspEI GGTNACC 1 cut(s) 1634
PspGI CCWGG 3 cut(s) 191, 733, 1630
PspLI CGTACG 1 cut(s) 309
PspN4I GGNNCC 3 cut(s) 136, 197, 999
PspPI GGNCC 5 cut(s) 134, 228, 427, 451, 806
PstNI CAGNNNCTG 1 cut(s) 809
PsuI RGATCY 2 cut(s) 997, 1519
PvuI CGATCG 1 cut(s) 273
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 4 cut(s) 242, 266, 305, 311
RsaNI GTAC 4 cut(s) 241, 265, 304, 310
RseI CAYNNNNRTG 5 cut(s) 444, 624, 1039, 1411, 1641
Rsr2I CGGWCCG 1 cut(s) 228
RsrII CGGWCCG 1 cut(s) 228
SaqAI TTAA 7 cut(s) 402, 900, 911, 978, 1047, 1167, 1505
SatI GCNGC 7 cut(s) 576, 609, 1020, 1368, 1418, 1421, 1434
Sau3AI GATC 8 cut(s) 270, 337, 529, 997, 1186, 1195, 1519, 1560
Sau96I GGNCC 5 cut(s) 134, 228, 427, 451, 806
SchI GAGTC 4 cut(s) 473, 680, 821, 1397
ScrFI CCNGG 4 cut(s) 106, 193, 735, 1632
SduI GDGCHC 2 cut(s) 98, 639
SexAI ACCWGGT 1 cut(s) 1630
SfaNI GCATC 6 cut(s) 562, 585, 1051, 1073, 1195, 1393
SfcI CTRYAG 2 cut(s) 1515, 1655
SinI GGWCC 3 cut(s) 134, 228, 451
SmiMI CAYNNNNRTG 5 cut(s) 444, 624, 1039, 1411, 1641
SmlI CTYRAG 4 cut(s) 599, 910, 1304, 1609
SmoI CTYRAG 4 cut(s) 599, 910, 1304, 1609
Sse9I AATT 9 cut(s) 250, 296, 658, 1199, 1209, 1283, 1388, 1445, 1574
SspMI CTAG 4 cut(s) 539, 1158, 1623, 1647
StyD4I CCNGG 4 cut(s) 104, 191, 733, 1630
StyI CCWWGG 3 cut(s) 941, 1040, 1277
TaaI ACNGT 4 cut(s) 641, 962, 1137, 1618
TaiI ACGT 4 cut(s) 311, 315, 777, 868
TaqI TCGA 6 cut(s) 255, 340, 674, 1080, 1242, 1449
TaqII GACCGA 2 cut(s) 245, 899
TasI AATT 9 cut(s) 250, 296, 658, 1199, 1209, 1283, 1388, 1445, 1574
TauI GCSGC 1 cut(s) 578
TfiI GAWTC 4 cut(s) 376, 535, 1072, 1292
Tru1I TTAA 7 cut(s) 402, 900, 911, 978, 1047, 1167, 1505
Tru9I TTAA 7 cut(s) 402, 900, 911, 978, 1047, 1167, 1505
TscAI CASTG 6 cut(s) 37, 58, 568, 769, 967, 1360
TseFI GTSAC 4 cut(s) 287, 641, 1618, 1634
TseI GCWGC 6 cut(s) 608, 1019, 1367, 1417, 1420, 1433
Tsp45I GTSAC 4 cut(s) 287, 641, 1618, 1634
TspDTI ATGAA 5 cut(s) 155, 156, 456, 662, 715
TspGWI ACGGA 3 cut(s) 147, 306, 1568
TspRI CASTG 6 cut(s) 37, 58, 568, 769, 967, 1360
Vha464I CTTAAG 1 cut(s) 910
VneI GTGCAC 1 cut(s) 635
VpaK11BI GGWCC 3 cut(s) 134, 228, 451
XapI RAATTY 4 cut(s) 250, 658, 1445, 1574
XbaI TCTAGA 1 cut(s) 538
XceI RCATGY 2 cut(s) 1105, 1382
XmiI GTMKAC 1 cut(s) 1582
XspI CTAG 4 cut(s) 539, 1158, 1623, 1647
ZraI GACGTC 1 cut(s) 866
Zsp2I ATGCAT 1 cut(s) 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.