Rroxscaffold_2G00143100

Calcium-dependent protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0006979
Physical Location & Seq
Forward (+)
81145500 .. 81149918
4419 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00143100.1

Sequence Viewer

Length: 1287 bp
ATGGTTCTACCTATAGCCGTTGAGGATGTTAAGCGAGAGGTCAAGATATTACAAGCGCTTGCAGGCCATGAGAATGTGGTTCGGTTCTATGATGCATTTGAAGATGATTCTTATGTGTATATAGTGATGGAATTATGTGAAGGGGGCGAGTTGCTGGATCGGATTTTGGAGAAGAAGGACAGCCGTTATTCTGAAAAAGATGCAGCAGTAGTTGTAAGGCAGATGCTCAAGGTTGCAGCTGAGTGTCATTTGCATGGTCTGGTGCACCGTGACATGAAACCTGAGAATTTTCTTTTTAAGTCTAAAGCATTGGACTCACCCTTGAAGGCGACAGACTTTGGTTTATCAGACTTTATTAAACCAGGGAAGAAATTTCAAGATATTGTTGGCAGTGCTTACTATGTTGCTCCCGAAGTGTTGAAGCGCAAGTCAGGACCTGAGTCAGATGTTTGGAGCATTGGTGTGATTACCTACATTTTGCTTTGTGGGCGACGCCCATTTTGGGATAAGACCGAAGATGGTATATTTAAGGAGGTCCTAAGGAACAAGCCTGATTTTCGTCGCAAACCATGGCCAACCATTAGCAACAGTGGAAAAGATTTTGTTAAGAAGTTATTGGTGAAGGACCCTCGGGCAAGGCTAACTGCTGCTCAGGCTTTATCACACCCATGGGTTAGAGAAGGAGGAGATGCATCAGACATTCCTATTGATATATCCGTCTTAAATAACATGCGACAGTTCGTGAGATACAGTCGTTTGAAACAGTTTGCTCTGAGGGCATTAGCTAGCACACTTAATGCGGAGGAGCTGGCTGATCTCAAAGATCAATTTGATGCAATTGATGTGGATAAGAATGGTTCTATTAGTCTTGAAGAAATGAGACAGGCACTTGCTAAAGATATCCCTTGGAAATTGAAAGATTCACGTGTTGTTGAGATTCTAGAAGCGATTGACAGCAACACAGATGGGCTTGTGGATTTCACTGAGTTTGTTGCAGCAACTCTGCATGTGCACCAATTGGAGGAACATGATTCTGAGAAGTGGCAGCAGCTGTCGCGTGCAGCTTTTGAGAAATTCGACATAGACAGAGATGGGTATATAACTCCAGACGAACTTAGAATGCACACGGGCCTGAAAGGTTCTATTGATCCACTTCTTGACGAGGCTGATATTGACAAAGATGGGAAGATTAGCCTATCAGAATTCCGTAGGCTCTTAAGAACTGCAAGCATGAGTTCACGAAACGTTACTAGCCCGATTGGTCAACGGAATTCTAGGCAGGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0002831 GO:0002832 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0004721 GO:0004722 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016311 GO:0016740 GO:0016772 GO:0016773 GO:0016787 GO:0016788 GO:0016791 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022603 GO:0023052 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043900 GO:0043901 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0048519 GO:0048583 GO:0048585 GO:0048831 GO:0050789 GO:0050793 GO:0050794 GO:0050832 GO:0050896 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060688 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080134 GO:0097305 GO:0097306 GO:0098542 GO:0140096 GO:1900424 GO:1900425 GO:1900618 GO:1901564 GO:1901700 GO:1901701 GO:1905428 GO:2000026 GO:2000032
KEGG Pathways
Metabolic & Signaling
ko04626 ko05145 map04626 map05145
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 Pkinase

Protein Analysis

428

Amino Acids

48.67

Weight (kDa)

5.84

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 8 - 217 1.7e-23 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 9 - 225 6.5e-62 Protein kinase domain
Kinase-like PF14531 51 - 175 4.6e-06 Kinase-like
EF-hand_7 PF13499 272 - 333 2.4e-12 EF-hand domain pair
EF-hand_5 PF13202 273 - 295 5.1e-06 EF hand
EF-hand_7 PF13499 353 - 406 1.4e-10 EF-hand domain pair
EF-hand_5 PF13202 354 - 373 1e-05 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1270
Acc36I ACCTGC 1 cut(s) 1270
AccII CGCG 1 cut(s) 1057
AciI CCGC 1 cut(s) 800
AclI AACGTT 1 cut(s) 1245
AclWI GGATC 2 cut(s) 165, 1142
AcoI YGGCCR 1 cut(s) 572
AcsI RAATTY 5 cut(s) 286, 371, 1073, 1202, 1270
AcvI CACGTG 1 cut(s) 926
AcyI GRCGYC 1 cut(s) 493
AfeI AGCGCT 1 cut(s) 57
AfiI CCNNNNNNNGG 2 cut(s) 502, 1021
AflII CTTAAG 1 cut(s) 1216
AflIII ACRYGT 1 cut(s) 925
AgsI TTSAA 7 cut(s) 101, 325, 377, 421, 760, 872, 916
AjnI CCWGG 1 cut(s) 361
AluBI AGCT 5 cut(s) 239, 785, 808, 1051, 1064
AluI AGCT 5 cut(s) 239, 785, 808, 1051, 1064
Alw21I GWGCWC 2 cut(s) 267, 1014
Alw26I GTCTC 1 cut(s) 874
Alw44I GTGCAC 2 cut(s) 263, 1010
AlwI GGATC 2 cut(s) 165, 1142
AlwNI CAGNNNCTG 2 cut(s) 437, 1051
Ama87I CYCGRG 1 cut(s) 630
Aor51HI AGCGCT 1 cut(s) 57
AoxI GGCC 3 cut(s) 64, 572, 1129
ApaLI GTGCAC 2 cut(s) 263, 1010
ApeKI GCWGC 7 cut(s) 203, 236, 647, 995, 1045, 1048, 1061
ApoI RAATTY 5 cut(s) 286, 371, 1073, 1202, 1270
ArsI GACNNNNNNTTYG 2 cut(s) 483, 515
AspLEI GCGC 2 cut(s) 58, 426
AspS9I GGNCC 4 cut(s) 434, 535, 625, 1129
AsuHPI GGTGA 2 cut(s) 309, 631
AsuNHI GCTAGC 1 cut(s) 785
AvaI CYCGRG 1 cut(s) 630
AvaII GGWCC 3 cut(s) 434, 535, 625
AxyI CCTNAGG 1 cut(s) 539
BaeGI GKGCMC 2 cut(s) 267, 1014
BaeI ACNNNNGTAYC 2 cut(s) 739, 772
BalI TGGCCA 1 cut(s) 574
BbrPI CACGTG 1 cut(s) 926
Bbv12I GWGCWC 2 cut(s) 267, 1014
BbvI GCAGC 7 cut(s) 215, 248, 634, 1007, 1057, 1060, 1073
BccI CCATC 5 cut(s) 121, 512, 959, 1085, 1175
BceAI ACGGC 2 cut(s) 2, 168
BciT130I CCWGG 1 cut(s) 363
BcoDI GTCTC 1 cut(s) 874
BfaI CTAG 4 cut(s) 786, 941, 1251, 1275
BfmI CTRYAG 1 cut(s) 12
BfoI RGCGCY 1 cut(s) 59
BfrI CTTAAG 1 cut(s) 1216
BfuAI ACCTGC 1 cut(s) 1270
BisI GCNGC 7 cut(s) 204, 237, 648, 996, 1046, 1049, 1062
BlsI GCNGC 7 cut(s) 205, 238, 649, 997, 1047, 1050, 1063
Bme1390I CCNGG 1 cut(s) 363
Bme18I GGWCC 3 cut(s) 434, 535, 625
BmeT110I CYCGRG 1 cut(s) 630
BmgT120I GGNCC 4 cut(s) 434, 535, 625, 1129
BmiI GGNNCC 1 cut(s) 627
BmrFI CCNGG 1 cut(s) 363
BmsI GCATC 6 cut(s) 82, 190, 213, 679, 701, 823
BmtI GCTAGC 1 cut(s) 789
BoxI GACNNNNGTC 1 cut(s) 439
BpmI CTGGAG 1 cut(s) 1089
Bpu10I CCTNAGC 1 cut(s) 651
BpuEI CTTGAG 1 cut(s) 212
BsaAI YACGTR 1 cut(s) 926
BsaHI GRCGYC 1 cut(s) 493
BsaJI CCNNGG 5 cut(s) 362, 569, 629, 668, 905
BsaXI ACNNNNNCTCC 2 cut(s) 445, 475
Bsc4I CCNNNNNNNGG 2 cut(s) 502, 1021
Bse21I CCTNAGG 1 cut(s) 539
BseBI CCWGG 1 cut(s) 363
BseDI CCNNGG 5 cut(s) 362, 569, 629, 668, 905
BseGI GGATG 1 cut(s) 31
BseLI CCNNNNNNNGG 2 cut(s) 502, 1021
BseMII CTCAG 7 cut(s) 231, 273, 429, 665, 764, 975, 1026
BseRI GAGGAG 2 cut(s) 699, 818
BseSI GKGCMC 2 cut(s) 267, 1014
BseXI GCAGC 7 cut(s) 215, 248, 634, 1007, 1057, 1060, 1073
BsgI GTGCAG 1 cut(s) 1080
Bsh1236I CGCG 1 cut(s) 1057
BshFI GGCC 3 cut(s) 66, 574, 1131
BsiHKAI GWGCWC 2 cut(s) 267, 1014
BsiHKCI CYCGRG 1 cut(s) 630
BslI CCNNNNNNNGG 2 cut(s) 502, 1021
BsmAI GTCTC 1 cut(s) 874
BsmI GAATGC 1 cut(s) 1125
BsnI GGCC 3 cut(s) 66, 574, 1131
BsoBI CYCGRG 1 cut(s) 630
Bsp1286I GDGCHC 2 cut(s) 267, 1014
Bsp143I GATC 4 cut(s) 157, 814, 823, 1147
Bsp19I CCATGG 2 cut(s) 569, 668
BspACI CCGC 1 cut(s) 800
BspANI GGCC 3 cut(s) 66, 574, 1131
BspCNI CTCAG 7 cut(s) 232, 274, 430, 664, 765, 976, 1027
BspFNI CGCG 1 cut(s) 1057
BspLI GGNNCC 1 cut(s) 627
BspMI ACCTGC 1 cut(s) 1270
BspOI GCTAGC 1 cut(s) 789
BspPI GGATC 2 cut(s) 165, 1142
BspTI CTTAAG 1 cut(s) 1216
BssECI CCNNGG 5 cut(s) 362, 569, 629, 668, 905
BssMI GATC 4 cut(s) 157, 814, 823, 1147
BssNI GRCGYC 1 cut(s) 493
BssT1I CCWWGG 3 cut(s) 569, 668, 905
Bst2UI CCWGG 1 cut(s) 363
Bst4CI ACNGT 5 cut(s) 269, 590, 738, 752, 765
BstACI GRCGYC 1 cut(s) 493
BstAFI CTTAAG 1 cut(s) 1216
BstBAI YACGTR 1 cut(s) 926
BstC8I GCNNGC 6 cut(s) 60, 64, 787, 810, 1059, 1228
BstDEI CTNAG 9 cut(s) 240, 282, 438, 539, 651, 773, 984, 1035, 1115
BstDSI CCRYGG 2 cut(s) 569, 668
BstF5I GGATG 1 cut(s) 31
BstFNI CGCG 1 cut(s) 1057
BstH2I RGCGCY 1 cut(s) 59
BstHHI GCGC 2 cut(s) 58, 426
BstKTI GATC 4 cut(s) 160, 817, 826, 1150
BstMAI GTCTC 1 cut(s) 874
BstMBI GATC 4 cut(s) 157, 814, 823, 1147
BstMWI GCNNNNNNNGC 4 cut(s) 487, 653, 776, 1054
BstNI CCWGG 1 cut(s) 363
BstNSI RCATGY 2 cut(s) 733, 1010
BstPAI GACNNNNGTC 1 cut(s) 439
BstSCI CCNGG 1 cut(s) 361
BstSFI CTRYAG 1 cut(s) 12
BstSLI GKGCMC 2 cut(s) 267, 1014
BstUI CGCG 1 cut(s) 1057
BstV1I GCAGC 7 cut(s) 215, 248, 634, 1007, 1057, 1060, 1073
Bsu36I CCTNAGG 1 cut(s) 539
BsuRI GGCC 3 cut(s) 66, 574, 1131
BtgI CCRYGG 2 cut(s) 569, 668
BtsCI GGATG 1 cut(s) 31
BtsI GCAGTG 1 cut(s) 397
BtsIMutI CAGTG 3 cut(s) 397, 595, 981
BveI ACCTGC 1 cut(s) 1270
Cac8I GCNNGC 6 cut(s) 60, 64, 787, 810, 1059, 1228
CaiI CAGNNNCTG 2 cut(s) 437, 1051
CfoI GCGC 2 cut(s) 58, 426
Cfr13I GGNCC 4 cut(s) 434, 535, 625, 1129
CseI GACGC 1 cut(s) 501
CspCI CAANNNNNGTGG 2 cut(s) 825, 860
CviAII CATG 9 cut(s) 68, 254, 274, 570, 669, 730, 1007, 1028, 1231
DdeI CTNAG 9 cut(s) 240, 282, 438, 539, 651, 773, 984, 1035, 1115
DpnI GATC 4 cut(s) 159, 816, 825, 1149
DpnII GATC 4 cut(s) 157, 814, 823, 1147
EaeI YGGCCR 1 cut(s) 572
Eco130I CCWWGG 3 cut(s) 569, 668, 905
Eco32I GATATC 1 cut(s) 901
Eco47I GGWCC 3 cut(s) 434, 535, 625
Eco47III AGCGCT 1 cut(s) 57
Eco72I CACGTG 1 cut(s) 926
Eco81I CCTNAGG 1 cut(s) 539
Eco88I CYCGRG 1 cut(s) 630
EcoO109I RGGNCCY 3 cut(s) 434, 535, 625
EcoRI GAATTC 2 cut(s) 1202, 1270
EcoRII CCWGG 1 cut(s) 361
EcoRV GATATC 1 cut(s) 901
EcoT14I CCWWGG 3 cut(s) 569, 668, 905
EcoT22I ATGCAT 2 cut(s) 97, 694
ErhI CCWWGG 3 cut(s) 569, 668, 905
FaeI CATG 9 cut(s) 71, 257, 277, 573, 672, 733, 1010, 1031, 1234
FatI CATG 9 cut(s) 67, 253, 273, 569, 668, 729, 1006, 1027, 1230
Fnu4HI GCNGC 7 cut(s) 204, 237, 648, 996, 1046, 1049, 1062
FokI GGATG 1 cut(s) 38
Fsp4HI GCNGC 7 cut(s) 204, 237, 648, 996, 1046, 1049, 1062
FspBI CTAG 4 cut(s) 786, 941, 1251, 1275
GlaI GCGC 2 cut(s) 57, 425
GluI GCNGC 7 cut(s) 204, 237, 648, 996, 1046, 1049, 1062
GsuI CTGGAG 1 cut(s) 1089
HaeII RGCGCY 1 cut(s) 59
HaeIII GGCC 3 cut(s) 66, 574, 1131
HgaI GACGC 1 cut(s) 501
HhaI GCGC 2 cut(s) 58, 426
Hin1I GRCGYC 1 cut(s) 493
Hin1II CATG 9 cut(s) 71, 257, 277, 573, 672, 733, 1010, 1031, 1234
Hin6I GCGC 2 cut(s) 56, 424
HinP1I GCGC 2 cut(s) 56, 424
HincII GTYRAC 1 cut(s) 1265
HindII GTYRAC 1 cut(s) 1265
HinfI GANTC 6 cut(s) 107, 314, 440, 920, 937, 1031
HphI GGTGA 2 cut(s) 309, 631
Hpy166II GTNNAC 4 cut(s) 265, 1012, 1238, 1265
Hpy188I TCNGA 8 cut(s) 162, 193, 349, 445, 697, 774, 1036, 1201
Hpy8I GTNNAC 4 cut(s) 265, 1012, 1238, 1265
Hpy99I CGWCG 2 cut(s) 495, 564
HpyAV CCTTC 5 cut(s) 134, 169, 319, 616, 674
HpyCH4III ACNGT 5 cut(s) 269, 590, 738, 752, 765
HpyCH4IV ACGT 2 cut(s) 925, 1245
HpyF10VI GCNNNNNNNGC 4 cut(s) 487, 653, 776, 1054
HpyF3I CTNAG 9 cut(s) 240, 282, 438, 539, 651, 773, 984, 1035, 1115
HpySE526I ACGT 2 cut(s) 925, 1245
Hsp92I GRCGYC 1 cut(s) 493
Hsp92II CATG 9 cut(s) 71, 257, 277, 573, 672, 733, 1010, 1031, 1234
HspAI GCGC 2 cut(s) 56, 424
Kzo9I GATC 4 cut(s) 157, 814, 823, 1147
LmnI GCTCC 3 cut(s) 412, 453, 805
Lsp1109I GCAGC 7 cut(s) 215, 248, 634, 1007, 1057, 1060, 1073
LweI GCATC 6 cut(s) 82, 190, 213, 679, 701, 823
MaeI CTAG 4 cut(s) 786, 941, 1251, 1275
MaeII ACGT 2 cut(s) 925, 1245
MaeIII GTNAC 2 cut(s) 269, 1246
MalI GATC 4 cut(s) 159, 816, 825, 1149
MboI GATC 4 cut(s) 157, 814, 823, 1147
MboII GAAGA 6 cut(s) 113, 184, 379, 527, 884, 1198
MfeI CAATTG 2 cut(s) 837, 1016
MhlI GDGCHC 2 cut(s) 267, 1014
MlsI TGGCCA 1 cut(s) 574
MluNI TGGCCA 1 cut(s) 574
MlyI GAGTC 2 cut(s) 308, 449
MnlI CCTC 9 cut(s) 16, 31, 526, 639, 677, 768, 796, 1015, 1156
Mox20I TGGCCA 1 cut(s) 574
Mph1103I ATGCAT 2 cut(s) 97, 694
MscI TGGCCA 1 cut(s) 574
MseI TTAA 8 cut(s) 30, 297, 357, 528, 606, 722, 795, 1217
MslI CAYNNNNRTG 4 cut(s) 72, 252, 461, 667
Msp20I TGGCCA 1 cut(s) 574
MspA1I CMGCKG 2 cut(s) 239, 1051
MspCI CTTAAG 1 cut(s) 1216
MspR9I CCNGG 1 cut(s) 363
MunI CAATTG 2 cut(s) 837, 1016
Mva1269I GAATGC 1 cut(s) 1125
MvaI CCWGG 1 cut(s) 363
MvnI CGCG 1 cut(s) 1057
MwoI GCNNNNNNNGC 4 cut(s) 487, 653, 776, 1054
NcoI CCATGG 2 cut(s) 569, 668
NdeII GATC 4 cut(s) 157, 814, 823, 1147
NheI GCTAGC 1 cut(s) 785
NlaIII CATG 9 cut(s) 71, 257, 277, 573, 672, 733, 1010, 1031, 1234
NlaIV GGNNCC 1 cut(s) 627
NmuCI GTSAC 1 cut(s) 269
NsiI ATGCAT 2 cut(s) 97, 694
NspI RCATGY 2 cut(s) 733, 1010
PaqCI CACCTGC 1 cut(s) 1270
PctI GAATGC 1 cut(s) 1125
PfeI GAWTC 4 cut(s) 107, 920, 937, 1031
PkrI GCNGC 7 cut(s) 205, 238, 649, 997, 1047, 1050, 1063
PleI GAGTC 2 cut(s) 308, 448
PmaCI CACGTG 1 cut(s) 926
PmlI CACGTG 1 cut(s) 926
PpsI GAGTC 2 cut(s) 308, 448
Ppu21I YACGTR 1 cut(s) 926
PpuMI RGGWCCY 3 cut(s) 434, 535, 625
PshAI GACNNNNGTC 1 cut(s) 439
Psp1406I AACGTT 1 cut(s) 1245
Psp5II RGGWCCY 3 cut(s) 434, 535, 625
Psp6I CCWGG 1 cut(s) 361
PspCI CACGTG 1 cut(s) 926
PspGI CCWGG 1 cut(s) 361
PspN4I GGNNCC 1 cut(s) 627
PspPI GGNCC 4 cut(s) 434, 535, 625, 1129
PspPPI RGGWCCY 3 cut(s) 434, 535, 625
PstNI CAGNNNCTG 2 cut(s) 437, 1051
PvuII CAGCTG 2 cut(s) 239, 1051
RseI CAYNNNNRTG 4 cut(s) 72, 252, 461, 667
SaqAI TTAA 8 cut(s) 30, 297, 357, 528, 606, 722, 795, 1217
SatI GCNGC 7 cut(s) 204, 237, 648, 996, 1046, 1049, 1062
Sau3AI GATC 4 cut(s) 157, 814, 823, 1147
Sau96I GGNCC 4 cut(s) 434, 535, 625, 1129
SchI GAGTC 2 cut(s) 308, 449
ScrFI CCNGG 1 cut(s) 363
SduI GDGCHC 2 cut(s) 267, 1014
SfaNI GCATC 6 cut(s) 82, 190, 213, 679, 701, 823
SfcI CTRYAG 1 cut(s) 12
SinI GGWCC 3 cut(s) 434, 535, 625
SmiMI CAYNNNNRTG 4 cut(s) 72, 252, 461, 667
SmlI CTYRAG 2 cut(s) 227, 1216
SmoI CTYRAG 2 cut(s) 227, 1216
SsiI CCGC 1 cut(s) 800
SspMI CTAG 4 cut(s) 786, 941, 1251, 1275
StyD4I CCNGG 1 cut(s) 361
StyI CCWWGG 3 cut(s) 569, 668, 905
TaaI ACNGT 5 cut(s) 269, 590, 738, 752, 765
TaiI ACGT 2 cut(s) 928, 1248
TaqI TCGA 1 cut(s) 1077
TaqII GACCGA 1 cut(s) 527
TfiI GAWTC 4 cut(s) 107, 920, 937, 1031
Tru1I TTAA 8 cut(s) 30, 297, 357, 528, 606, 722, 795, 1217
Tru9I TTAA 8 cut(s) 30, 297, 357, 528, 606, 722, 795, 1217
TscAI CASTG 3 cut(s) 397, 595, 988
TseFI GTSAC 1 cut(s) 269
TseI GCWGC 7 cut(s) 203, 236, 647, 995, 1045, 1048, 1061
Tsp45I GTSAC 1 cut(s) 269
TspDTI ATGAA 1 cut(s) 290
TspGWI ACGGA 3 cut(s) 706, 1196, 1282
TspRI CASTG 3 cut(s) 397, 595, 988
Vha464I CTTAAG 1 cut(s) 1216
VneI GTGCAC 2 cut(s) 263, 1010
VpaK11BI GGWCC 3 cut(s) 434, 535, 625
XapI RAATTY 5 cut(s) 286, 371, 1073, 1202, 1270
XbaI TCTAGA 1 cut(s) 940
XceI RCATGY 2 cut(s) 733, 1010
XspI CTAG 4 cut(s) 786, 941, 1251, 1275
Zsp2I ATGCAT 2 cut(s) 97, 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.