MD02G1132800.v1.1

WEB family protein At3g51220-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014992
Physical Location & Seq
Forward (+)
10742754 .. 10744061
1308 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1132800.v1.1.491

Sequence Viewer

Length: 669 bp
ATGAATGCCGACGAGGGTAAACTGGTGGTCATGGGCCGGGCAGAGATTGACACCCGAGCACCTTTCCGGTCAGTCAAAGAAGCAGTTATGTTGTTCGGCGAAAGAGTTTTGGTCGGAGAAATATATGCCAATAAACTCAAAGAGATTGGAGCTGCAGGGTCAAATGACACTAATGGACATGCTGCTCCGTCGAGGATCGAGGTCTTAATGGCCGAGCTCGAAGAAACAAAGAAAAGTCTACAGAAAGTTAGAGAAGAAAACAACTTAATGGCATACAGCATCAAGTCACTCAGAGAAGATCTTGAACATTCAAAACAAGAGCTCCACAAGTACTTGAAGGCAAGAGAGTTTGATGATCAGAAGCACCGAGTTGATCCTGAGATTGAAGAAGATTTTAAGTTCATCGCGACCGGAGACACGAAGTTTGAAACCAAAAAAATGTTGAGCCAAGAGGCTGAGGAATTTGTGCAGAAGGAAAGACATGTGAAGTTTGCAAGTCCTCCTTCGTTGGCTCAGGTTATTGTTAACAAAGAAGGGTCGCATGAGGGATATGAAAACCCTAGTTCTGTTAGAAAGCCAAATAAGAAGAAGCCTTTGATGCCTATGATGATTGGGTGGCTTTTTCGCAAAAGTAAGGCAATTCAGGAAGGTGGGTCTCCGAGGGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

223

Amino Acids

25.23

Weight (kDa)

7.86

Isoelectric Point (pI)

45.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014992)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51220
fragaria_vesca FvH4_1g11870
malus_domestica MD02G1132800.v1.1 MD15G1245800.v1.1
pyrus_communis pycom02g10420 pycom15g21630
rosa_chinensis RchiOBHm_Chr2g0099471
rosa_laevigata RLG00000016885
rosa_multiflora Rmu_sc0002542.1_g000014
rosa_roxburghii Rroxscaffold_2G00143050
rosa_rugosa Rorug02G0079900
rosa_samantha Rh2AG129900 Rh2BG133000 Rh2CG133600 Rh2DG133900
rosa_wichuraiana Rw2G010000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 238
AccII CGCG 1 cut(s) 407
AclWI GGATC 2 cut(s) 203, 368
AcoI YGGCCR 1 cut(s) 210
AcsI RAATTY 1 cut(s) 461
AfaI GTAC 1 cut(s) 332
AflIII ACRYGT 1 cut(s) 481
AgsI TTSAA 5 cut(s) 305, 312, 337, 386, 428
AluBI AGCT 3 cut(s) 152, 217, 322
AluI AGCT 3 cut(s) 152, 217, 322
Alw21I GWGCWC 3 cut(s) 61, 219, 324
Alw26I GTCTC 2 cut(s) 408, 660
AlwI GGATC 2 cut(s) 203, 368
Ama87I CYCGRG 1 cut(s) 54
AoxI GGCC 2 cut(s) 34, 210
ApeKI GCWGC 2 cut(s) 152, 182
ApoI RAATTY 1 cut(s) 461
ArsI GACNNNNNNTTYG 2 cut(s) 407, 439
AspS9I GGNCC 1 cut(s) 34
AsuC2I CCSGG 1 cut(s) 38
AvaI CYCGRG 1 cut(s) 54
BanII GRGCYC 2 cut(s) 219, 324
Bbv12I GWGCWC 3 cut(s) 61, 219, 324
BbvCI CCTCAGC 1 cut(s) 456
BbvI GCAGC 2 cut(s) 139, 169
BclI TGATCA 1 cut(s) 355
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 2 cut(s) 408, 660
BfaI CTAG 1 cut(s) 561
BfmI CTRYAG 2 cut(s) 153, 239
BglII AGATCT 1 cut(s) 298
BisI GCNGC 2 cut(s) 153, 183
BlsI GCNGC 2 cut(s) 154, 184
BmcAI AGTACT 1 cut(s) 332
Bme1390I CCNGG 1 cut(s) 38
BmeT110I CYCGRG 1 cut(s) 54
BmgT120I GGNCC 1 cut(s) 34
BmrFI CCNGG 1 cut(s) 38
BmsI GCATC 2 cut(s) 288, 588
Bpu10I CCTNAGC 2 cut(s) 456, 513
BpuMI CCSGG 1 cut(s) 38
BsaI GGTCTC 1 cut(s) 660
BsaJI CCNNGG 1 cut(s) 659
BsaWI WCCGGW 2 cut(s) 66, 410
BsaXI ACNNNNNCTCC 2 cut(s) 306, 336
Bse1I ACTGG 1 cut(s) 27
BseDI CCNNGG 1 cut(s) 659
BseMII CTCAG 4 cut(s) 304, 369, 447, 527
BseNI ACTGG 1 cut(s) 27
BseXI GCAGC 2 cut(s) 139, 169
BsgI GTGCAG 1 cut(s) 488
Bsh1236I CGCG 1 cut(s) 407
Bsh1285I CGRYCG 1 cut(s) 411
BshFI GGCC 2 cut(s) 36, 212
BsiEI CGRYCG 1 cut(s) 411
BsiHKAI GWGCWC 3 cut(s) 61, 219, 324
BsiHKCI CYCGRG 1 cut(s) 54
BsiSI CCGG 3 cut(s) 37, 67, 411
BsmAI GTCTC 2 cut(s) 408, 660
BsmI GAATGC 1 cut(s) 10
BsnI GGCC 2 cut(s) 36, 212
Bso31I GGTCTC 1 cut(s) 660
BsoBI CYCGRG 1 cut(s) 54
Bsp1286I GDGCHC 3 cut(s) 61, 219, 324
Bsp143I GATC 4 cut(s) 195, 298, 355, 373
Bsp68I TCGCGA 1 cut(s) 407
BspANI GGCC 2 cut(s) 36, 212
BspCNI CTCAG 4 cut(s) 303, 370, 448, 526
BspFNI CGCG 1 cut(s) 407
BspMAI CTGCAG 1 cut(s) 157
BspPI GGATC 2 cut(s) 203, 368
BspTNI GGTCTC 1 cut(s) 660
BsrI ACTGG 1 cut(s) 27
BssECI CCNNGG 1 cut(s) 659
BssMI GATC 4 cut(s) 195, 298, 355, 373
BstDEI CTNAG 4 cut(s) 290, 378, 456, 513
BstFNI CGCG 1 cut(s) 407
BstKTI GATC 4 cut(s) 198, 301, 358, 376
BstMAI GTCTC 2 cut(s) 408, 660
BstMBI GATC 4 cut(s) 195, 298, 355, 373
BstMCI CGRYCG 1 cut(s) 411
BstMWI GCNNNNNNNGC 1 cut(s) 598
BstNSI RCATGY 2 cut(s) 182, 485
BstSCI CCNGG 1 cut(s) 36
BstSFI CTRYAG 2 cut(s) 153, 239
BstUI CGCG 1 cut(s) 407
BstV1I GCAGC 2 cut(s) 139, 169
BstX2I RGATCY 1 cut(s) 298
BstYI RGATCY 1 cut(s) 298
BsuRI GGCC 2 cut(s) 36, 212
BtgZI GCGATG 1 cut(s) 388
BtuMI TCGCGA 1 cut(s) 407
Cfr13I GGNCC 1 cut(s) 34
Csp6I GTAC 1 cut(s) 331
CviAII CATG 4 cut(s) 31, 179, 482, 542
CviQI GTAC 1 cut(s) 331
DdeI CTNAG 4 cut(s) 290, 378, 456, 513
DpnI GATC 4 cut(s) 197, 300, 357, 375
DpnII GATC 4 cut(s) 195, 298, 355, 373
EaeI YGGCCR 1 cut(s) 210
Ecl136II GAGCTC 2 cut(s) 217, 322
Eco24I GRGCYC 2 cut(s) 219, 324
Eco31I GGTCTC 1 cut(s) 660
Eco53kI GAGCTC 2 cut(s) 217, 322
Eco88I CYCGRG 1 cut(s) 54
EcoICRI GAGCTC 2 cut(s) 217, 322
EcoT38I GRGCYC 2 cut(s) 219, 324
FaeI CATG 4 cut(s) 34, 182, 485, 545
FalI AAGNNNNNCTT 2 cut(s) 487, 519
FatI CATG 4 cut(s) 30, 178, 481, 541
FbaI TGATCA 1 cut(s) 355
FblI GTMKAC 1 cut(s) 238
Fnu4HI GCNGC 2 cut(s) 153, 183
FriOI GRGCYC 2 cut(s) 219, 324
Fsp4HI GCNGC 2 cut(s) 153, 183
FspBI CTAG 1 cut(s) 561
GluI GCNGC 2 cut(s) 153, 183
HaeIII GGCC 2 cut(s) 36, 212
HapII CCGG 3 cut(s) 37, 67, 411
Hin1II CATG 4 cut(s) 34, 182, 485, 545
HincII GTYRAC 1 cut(s) 526
HindII GTYRAC 1 cut(s) 526
HpaI GTTAAC 1 cut(s) 526
HpaII CCGG 3 cut(s) 37, 67, 411
Hpy166II GTNNAC 3 cut(s) 20, 239, 526
Hpy188I TCNGA 4 cut(s) 116, 293, 360, 660
Hpy188III TCNNGA 4 cut(s) 302, 377, 406, 644
Hpy8I GTNNAC 3 cut(s) 20, 239, 526
Hpy99I CGWCG 2 cut(s) 14, 193
HpyAV CCTTC 5 cut(s) 331, 466, 513, 527, 641
HpyCH4V TGCA 3 cut(s) 155, 469, 494
HpyF10VI GCNNNNNNNGC 1 cut(s) 598
HpyF3I CTNAG 4 cut(s) 290, 378, 456, 513
Hsp92II CATG 4 cut(s) 34, 182, 485, 545
Ksp22I TGATCA 1 cut(s) 355
KspAI GTTAAC 1 cut(s) 526
Kzo9I GATC 4 cut(s) 195, 298, 355, 373
LmnI GCTCC 3 cut(s) 149, 190, 327
LpnPI CCDG 8 cut(s) 8, 50, 80, 141, 390, 424, 500, 629
Lsp1109I GCAGC 2 cut(s) 139, 169
LweI GCATC 2 cut(s) 288, 588
MaeI CTAG 1 cut(s) 561
MaeIII GTNAC 1 cut(s) 285
MalI GATC 4 cut(s) 197, 300, 357, 375
MboI GATC 4 cut(s) 195, 298, 355, 373
MboII GAAGA 6 cut(s) 233, 266, 308, 398, 401, 598
MflI RGATCY 1 cut(s) 298
MhlI GDGCHC 3 cut(s) 61, 219, 324
MluCI AATT 2 cut(s) 461, 639
MmeI TCCRAC 1 cut(s) 94
MnlI CCTC 8 cut(s) 7, 186, 193, 445, 451, 510, 538, 654
MseI TTAA 4 cut(s) 206, 266, 396, 525
MspI CCGG 3 cut(s) 37, 67, 411
MspR9I CCNGG 1 cut(s) 38
Mva1269I GAATGC 1 cut(s) 10
MvnI CGCG 1 cut(s) 407
MwoI GCNNNNNNNGC 1 cut(s) 598
NciI CCSGG 1 cut(s) 38
NdeII GATC 4 cut(s) 195, 298, 355, 373
NlaIII CATG 4 cut(s) 34, 182, 485, 545
NmeAIII GCCGAG 1 cut(s) 238
NmuCI GTSAC 1 cut(s) 285
NruI TCGCGA 1 cut(s) 407
NspI RCATGY 2 cut(s) 182, 485
PciI ACATGT 1 cut(s) 481
PctI GAATGC 1 cut(s) 10
PkrI GCNGC 2 cut(s) 154, 184
PscI ACATGT 1 cut(s) 481
Psp124BI GAGCTC 2 cut(s) 219, 324
PspPI GGNCC 1 cut(s) 34
PstI CTGCAG 1 cut(s) 157
PsuI RGATCY 1 cut(s) 298
RruI TCGCGA 1 cut(s) 407
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
SacI GAGCTC 2 cut(s) 219, 324
SaqAI TTAA 4 cut(s) 206, 266, 396, 525
SatI GCNGC 2 cut(s) 153, 183
Sau3AI GATC 4 cut(s) 195, 298, 355, 373
Sau96I GGNCC 1 cut(s) 34
ScaI AGTACT 1 cut(s) 332
ScrFI CCNGG 1 cut(s) 38
SduI GDGCHC 3 cut(s) 61, 219, 324
SetI ASST 7 cut(s) 64, 154, 204, 219, 324, 519, 652
SfaNI GCATC 2 cut(s) 288, 588
SfcI CTRYAG 2 cut(s) 153, 239
Sse9I AATT 2 cut(s) 461, 639
SspMI CTAG 1 cut(s) 561
SstI GAGCTC 2 cut(s) 219, 324
StyD4I CCNGG 1 cut(s) 36
TaqI TCGA 3 cut(s) 191, 198, 219
TasI AATT 2 cut(s) 461, 639
TatI WGTACW 1 cut(s) 330
Tru1I TTAA 4 cut(s) 206, 266, 396, 525
Tru9I TTAA 4 cut(s) 206, 266, 396, 525
TseFI GTSAC 1 cut(s) 285
TseI GCWGC 2 cut(s) 152, 182
Tsp45I GTSAC 1 cut(s) 285
TspDTI ATGAA 3 cut(s) 17, 391, 567
TspGWI ACGGA 1 cut(s) 177
XapI RAATTY 1 cut(s) 461
XceI RCATGY 2 cut(s) 182, 485
XmiI GTMKAC 1 cut(s) 238
XspI CTAG 1 cut(s) 561
ZrmI AGTACT 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.