MD15G1245800.v1.1

WEB family protein At3g51220-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014992
Physical Location & Seq
Reverse (-)
20325415 .. 20326847
1433 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1245800.v1.1.491

Sequence Viewer

Length: 669 bp
ATGAACGCCGACGAGAGTAAATTGGTTGTCATGGGCCGGGCAGAGATCGACACCCGAGCGCCTTTCAAGTCCGTCAAAGAAGCGGTCATGTTGTTCGGGGAAAGAGTTTTGGTCGGAGAAATATATGCCAAGCAGCTCAAAGAGATTGGAGCTGCAGTGTCCAATGATACTACTAATGGAGATGCTGCTCAGTCAAGAATCGAGGTCTTAACATCGGAGCTCGAAGAAACAAAGAAAAGTCTTCAGAAAGTTAGAGAAGAAAACAAGTCAATGGCATATTGCATCAAGTCACTCAGAGAAGATCTTGACCGTTCAAAACAAGAGCTCCACAAGTACTTGAAGGCAAGAGAGTTTGATGATCAGAAGCACCGAGTAGATCCTGAGATTGAAGAAGATTTTAAGTTCATTGCGGACAGAGACACGAAGTTCGAAAACAAGAAAATGTTGAGCCAAGAGGCTGAGGAGTTTGTGCAGAAGAAAAGATATGTGAAGTTTGCAAGTCCTGCTTCGTTGGCTCAGGTTATTGTTAACAAAGAAGGGTCGCTTGAGGGATATGGAAACCCTAGTTCGGTCAGGAAAACAAATAAGAAGAAGCCTTTGATGCCTATGATTGGGTGGCTTTTTTCAAAAAATAAGGCAATTCAAGAAGGTGAGTCTTCAAGAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

223

Amino Acids

25.31

Weight (kDa)

8.73

Isoelectric Point (pI)

48.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014992)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51220
fragaria_vesca FvH4_1g11870
malus_domestica MD02G1132800.v1.1 MD15G1245800.v1.1
pyrus_communis pycom02g10420 pycom15g21630
rosa_chinensis RchiOBHm_Chr2g0099471
rosa_laevigata RLG00000016885
rosa_multiflora Rmu_sc0002542.1_g000014
rosa_roxburghii Rroxscaffold_2G00143050
rosa_rugosa Rorug02G0079900
rosa_samantha Rh2AG129900 Rh2BG133000 Rh2CG133600 Rh2DG133900
rosa_wichuraiana Rw2G010000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 83, 410
AclWI GGATC 1 cut(s) 371
AcuI CTGAAG 1 cut(s) 227
AfaI GTAC 1 cut(s) 335
AfiI CCNNNNNNNGG 2 cut(s) 568, 611
AgsI TTSAA 7 cut(s) 67, 315, 340, 389, 627, 644, 660
AluBI AGCT 4 cut(s) 136, 152, 220, 325
AluI AGCT 4 cut(s) 136, 152, 220, 325
Alw21I GWGCWC 2 cut(s) 222, 327
Alw26I GTCTC 1 cut(s) 411
AlwI GGATC 1 cut(s) 371
Ama87I CYCGRG 1 cut(s) 54
AoxI GGCC 1 cut(s) 34
ApeKI GCWGC 3 cut(s) 133, 152, 185
ArsI GACNNNNNNTTYG 4 cut(s) 69, 101, 410, 442
AspLEI GCGC 1 cut(s) 61
AspS9I GGNCC 1 cut(s) 34
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 1 cut(s) 662
AsuII TTCGAA 1 cut(s) 429
AvaI CYCGRG 1 cut(s) 54
BanII GRGCYC 2 cut(s) 222, 327
BbsI GAAGAC 2 cut(s) 233, 648
Bbv12I GWGCWC 2 cut(s) 222, 327
BbvCI CCTCAGC 1 cut(s) 459
BbvI GCAGC 3 cut(s) 139, 145, 172
BclI TGATCA 1 cut(s) 358
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 1 cut(s) 411
BfaI CTAG 1 cut(s) 564
BfmI CTRYAG 1 cut(s) 153
BfoI RGCGCY 1 cut(s) 62
BglII AGATCT 1 cut(s) 301
BisI GCNGC 3 cut(s) 134, 153, 186
BlsI GCNGC 3 cut(s) 135, 154, 187
BmcAI AGTACT 1 cut(s) 335
Bme1390I CCNGG 1 cut(s) 38
BmeT110I CYCGRG 1 cut(s) 54
BmgT120I GGNCC 1 cut(s) 34
BmrFI CCNGG 1 cut(s) 38
BmsI GCATC 3 cut(s) 172, 291, 591
BpiI GAAGAC 2 cut(s) 233, 648
Bpu10I CCTNAGC 2 cut(s) 459, 516
Bpu14I TTCGAA 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 566
BpuMI CCSGG 1 cut(s) 38
BsaXI ACNNNNNCTCC 4 cut(s) 141, 171, 309, 339
Bsc4I CCNNNNNNNGG 2 cut(s) 568, 611
Bse3DI GCAATG 1 cut(s) 405
BseLI CCNNNNNNNGG 2 cut(s) 568, 611
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 5 cut(s) 203, 307, 372, 450, 530
BseRI GAGGAG 1 cut(s) 476
BseXI GCAGC 3 cut(s) 139, 145, 172
BsgI GTGCAG 1 cut(s) 491
BshFI GGCC 1 cut(s) 36
BsiHKAI GWGCWC 2 cut(s) 222, 327
BsiHKCI CYCGRG 1 cut(s) 54
BsiSI CCGG 1 cut(s) 37
BslI CCNNNNNNNGG 2 cut(s) 568, 611
BsmAI GTCTC 1 cut(s) 411
BsnI GGCC 1 cut(s) 36
BsoBI CYCGRG 1 cut(s) 54
Bsp119I TTCGAA 1 cut(s) 429
Bsp1286I GDGCHC 2 cut(s) 222, 327
Bsp143I GATC 4 cut(s) 45, 301, 358, 376
BspACI CCGC 2 cut(s) 83, 410
BspANI GGCC 1 cut(s) 36
BspCNI CTCAG 5 cut(s) 202, 306, 373, 451, 529
BspMAI CTGCAG 1 cut(s) 157
BspPI GGATC 1 cut(s) 371
BspT104I TTCGAA 1 cut(s) 429
BsrDI GCAATG 1 cut(s) 405
BssMI GATC 4 cut(s) 45, 301, 358, 376
Bst4CI ACNGT 1 cut(s) 311
BstAPI GCANNNNNTGC 1 cut(s) 503
BstBI TTCGAA 1 cut(s) 429
BstDEI CTNAG 5 cut(s) 189, 293, 381, 459, 516
BstH2I RGCGCY 1 cut(s) 62
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 4 cut(s) 48, 304, 361, 379
BstMAI GTCTC 1 cut(s) 411
BstMBI GATC 4 cut(s) 45, 301, 358, 376
BstMWI GCNNNNNNNGC 3 cut(s) 503, 512, 601
BstSCI CCNGG 1 cut(s) 36
BstSFI CTRYAG 1 cut(s) 153
BstV1I GCAGC 3 cut(s) 139, 145, 172
BstV2I GAAGAC 2 cut(s) 233, 648
BstX2I RGATCY 2 cut(s) 301, 376
BstYI RGATCY 2 cut(s) 301, 376
BsuRI GGCC 1 cut(s) 36
BtsI GCAGTG 1 cut(s) 162
BtsIMutI CAGTG 1 cut(s) 162
CfoI GCGC 1 cut(s) 61
Cfr13I GGNCC 1 cut(s) 34
Csp6I GTAC 1 cut(s) 334
CviAII CATG 2 cut(s) 31, 88
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 5 cut(s) 189, 293, 381, 459, 516
DpnI GATC 4 cut(s) 47, 303, 360, 378
DpnII GATC 4 cut(s) 45, 301, 358, 376
Ecl136II GAGCTC 2 cut(s) 220, 325
Eco24I GRGCYC 2 cut(s) 222, 327
Eco53kI GAGCTC 2 cut(s) 220, 325
Eco57I CTGAAG 1 cut(s) 227
Eco88I CYCGRG 1 cut(s) 54
EcoICRI GAGCTC 2 cut(s) 220, 325
EcoT38I GRGCYC 2 cut(s) 222, 327
FaeI CATG 2 cut(s) 34, 91
FaiI YATR 8 cut(s) 32, 89, 124, 126, 277, 486, 555, 608
FalI AAGNNNNNCTT 4 cut(s) 490, 522, 528, 560
FatI CATG 2 cut(s) 30, 87
FbaI TGATCA 1 cut(s) 358
Fnu4HI GCNGC 3 cut(s) 134, 153, 186
FriOI GRGCYC 2 cut(s) 222, 327
Fsp4HI GCNGC 3 cut(s) 134, 153, 186
FspBI CTAG 1 cut(s) 564
GlaI GCGC 1 cut(s) 60
GluI GCNGC 3 cut(s) 134, 153, 186
HaeII RGCGCY 1 cut(s) 62
HaeIII GGCC 1 cut(s) 36
HapII CCGG 1 cut(s) 37
HhaI GCGC 1 cut(s) 61
Hin1II CATG 2 cut(s) 34, 91
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HincII GTYRAC 1 cut(s) 529
HindII GTYRAC 1 cut(s) 529
HinfI GANTC 2 cut(s) 198, 653
HpaI GTTAAC 1 cut(s) 529
HpaII CCGG 1 cut(s) 37
HphI GGTGA 1 cut(s) 662
Hpy166II GTNNAC 1 cut(s) 529
Hpy188I TCNGA 5 cut(s) 116, 217, 246, 296, 363
Hpy188III TCNNGA 6 cut(s) 195, 305, 380, 574, 644, 660
Hpy8I GTNNAC 1 cut(s) 529
Hpy99I CGWCG 1 cut(s) 14
HpyAV CCTTC 3 cut(s) 334, 530, 641
HpyCH4III ACNGT 1 cut(s) 311
HpyCH4V TGCA 4 cut(s) 155, 282, 472, 497
HpyF10VI GCNNNNNNNGC 3 cut(s) 503, 512, 601
HpyF3I CTNAG 5 cut(s) 189, 293, 381, 459, 516
Hsp92II CATG 2 cut(s) 34, 91
HspAI GCGC 1 cut(s) 59
Ksp22I TGATCA 1 cut(s) 358
KspAI GTTAAC 1 cut(s) 529
Kzo9I GATC 4 cut(s) 45, 301, 358, 376
LmnI GCTCC 3 cut(s) 149, 217, 330
LpnPI CCDG 5 cut(s) 50, 393, 503, 516, 559
Lsp1109I GCAGC 3 cut(s) 139, 145, 172
LweI GCATC 3 cut(s) 172, 291, 591
MaeI CTAG 1 cut(s) 564
MaeIII GTNAC 1 cut(s) 288
MalI GATC 4 cut(s) 47, 303, 360, 378
MboI GATC 4 cut(s) 45, 301, 358, 376
MboII GAAGA 9 cut(s) 233, 236, 269, 311, 401, 404, 487, 601, 648
MflI RGATCY 2 cut(s) 301, 376
MhlI GDGCHC 2 cut(s) 222, 327
MluCI AATT 2 cut(s) 20, 639
MlyI GAGTC 1 cut(s) 662
MmeI TCCRAC 1 cut(s) 94
MnlI CCTC 4 cut(s) 196, 448, 454, 541
MseI TTAA 3 cut(s) 209, 399, 528
MspI CCGG 1 cut(s) 37
MspR9I CCNGG 1 cut(s) 38
MwoI GCNNNNNNNGC 3 cut(s) 503, 512, 601
NciI CCSGG 1 cut(s) 38
NdeII GATC 4 cut(s) 45, 301, 358, 376
NlaIII CATG 2 cut(s) 34, 91
NmuCI GTSAC 1 cut(s) 288
NspV TTCGAA 1 cut(s) 429
PfeI GAWTC 1 cut(s) 198
PkrI GCNGC 3 cut(s) 135, 154, 187
PleI GAGTC 1 cut(s) 661
PpsI GAGTC 1 cut(s) 661
Psp124BI GAGCTC 2 cut(s) 222, 327
PspPI GGNCC 1 cut(s) 34
PstI CTGCAG 1 cut(s) 157
PsuI RGATCY 2 cut(s) 301, 376
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
SacI GAGCTC 2 cut(s) 222, 327
SaqAI TTAA 3 cut(s) 209, 399, 528
SatI GCNGC 3 cut(s) 134, 153, 186
Sau3AI GATC 4 cut(s) 45, 301, 358, 376
Sau96I GGNCC 1 cut(s) 34
ScaI AGTACT 1 cut(s) 335
SchI GAGTC 1 cut(s) 662
ScrFI CCNGG 1 cut(s) 38
SduI GDGCHC 2 cut(s) 222, 327
SetI ASST 7 cut(s) 138, 154, 207, 222, 327, 522, 652
SfaNI GCATC 3 cut(s) 172, 291, 591
SfcI CTRYAG 1 cut(s) 153
SfuI TTCGAA 1 cut(s) 429
SmlI CTYRAG 1 cut(s) 545
SmoI CTYRAG 1 cut(s) 545
Sse9I AATT 2 cut(s) 20, 639
SsiI CCGC 2 cut(s) 83, 410
SspMI CTAG 1 cut(s) 564
SstI GAGCTC 2 cut(s) 222, 327
StyD4I CCNGG 1 cut(s) 36
TaaI ACNGT 1 cut(s) 311
TaqI TCGA 4 cut(s) 48, 201, 222, 429
TaqII GACCGA 1 cut(s) 559
TasI AATT 2 cut(s) 20, 639
TatI WGTACW 1 cut(s) 333
TfiI GAWTC 1 cut(s) 198
Tru1I TTAA 3 cut(s) 209, 399, 528
Tru9I TTAA 3 cut(s) 209, 399, 528
TscAI CASTG 1 cut(s) 162
TseFI GTSAC 1 cut(s) 288
TseI GCWGC 3 cut(s) 133, 152, 185
Tsp45I GTSAC 1 cut(s) 288
TspDTI ATGAA 2 cut(s) 17, 394
TspGWI ACGGA 1 cut(s) 61
TspRI CASTG 1 cut(s) 162
XspI CTAG 1 cut(s) 564
ZrmI AGTACT 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.