MD02G1133200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0016624
Physical Location & Seq
Forward (+)
10776820 .. 10777371
552 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1133200.v1.1.491

Sequence Viewer

Length: 552 bp
ATGGAGGCCAAACTCCTCCACCTACTCAGCTCAACACCAGCTTCCCCAACCCACCTCGCCCTCTCAAAACCTTCTCCAACAAAACTGAACCATATCTTCTCACCCGCCACCCGTCCGCGGCCTCTCAGAATCAGCACACTAGTTTACAACACTACCGGCAGTGATGGTGGTGGCAGCACTAGCATTCCGGACACTGACGGCAACAGCGTGGCAGCACCGGACCCCACGGGAGTGCGGTTCAAAAAGAGGTCGAGAAGGCGGACGAAGCAGCAGCAGCGAGAGGAGGGCAATGAGGGGGACGGCGGACGGGTCATGAAGGCTCAAGCTAGTGACGCTCCAAAGAAGTGGGAAGATATGAGCTTGGGGGAGAAGGCATGGGAGCTTTACGTGGGGGAGAAGGGTGCTTTGTTTTGGTTAAACAAGTTTGCTTATGCTTCAATTTACATAGTGATTGGGGCTTGGATTCTGTTCAGGTTTGTTGGACCGGCTCTTAATCTTTACCAGTTGGATGCACCTCCTCTCTCTCCCACTTCCATCCTCAAGGGTTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2839

Protein Analysis

184

Amino Acids

19.97

Weight (kDa)

9.8

Isoelectric Point (pI)

50.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016624)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G17972
fragaria_vesca FvH4_1g11940
malus_domestica MD02G1133200.v1.1
prunus_persica Prupe.7G167500_v2.0.a1
pyrus_communis pycom02g10470
rosa_laevigata RLG00000016890
rosa_multiflora Rmu_sc0002542.1_g000019
rosa_roxburghii Rroxscaffold_2G00142990
rosa_rugosa Rorug02G0080500
rosa_samantha Rh2AG130400 Rh2BG133500 Rh2CG134000 Rh2DG134300
rosa_wichuraiana Rw2G010060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 118
AccIII TCCGGA 1 cut(s) 187
AciI CCGC 6 cut(s) 105, 116, 118, 235, 259, 303
AfiI CCNNNNNNNGG 1 cut(s) 117
AgsI TTSAA 2 cut(s) 241, 438
AhlI ACTAGT 1 cut(s) 139
AleI CACNNNNGTG 1 cut(s) 230
AluBI AGCT 5 cut(s) 30, 41, 326, 360, 382
AluI AGCT 5 cut(s) 30, 41, 326, 360, 382
Aor13HI TCCGGA 1 cut(s) 187
AoxI GGCC 2 cut(s) 6, 119
ApeKI GCWGC 5 cut(s) 174, 212, 268, 271, 274
AspS9I GGNCC 2 cut(s) 220, 482
AsuHPI GGTGA 1 cut(s) 93
AvaII GGWCC 2 cut(s) 220, 482
BbvI GCAGC 5 cut(s) 186, 224, 280, 283, 286
BccI CCATC 2 cut(s) 158, 542
BceAI ACGGC 2 cut(s) 214, 316
BcuI ACTAGT 1 cut(s) 139
BfaI CTAG 3 cut(s) 140, 180, 327
BisI GCNGC 6 cut(s) 119, 175, 213, 269, 272, 275
BlsI GCNGC 6 cut(s) 120, 176, 214, 270, 273, 276
Bme18I GGWCC 2 cut(s) 220, 482
BmgT120I GGNCC 2 cut(s) 220, 482
BmiI GGNNCC 2 cut(s) 222, 547
BmsI GCATC 1 cut(s) 499
BpuEI CTTGAG 2 cut(s) 306, 524
BsaAI YACGTR 1 cut(s) 388
BsaJI CCNNGG 2 cut(s) 116, 225
BsaWI WCCGGW 2 cut(s) 187, 217
Bsc4I CCNNNNNNNGG 1 cut(s) 117
Bse118I RCCGGY 2 cut(s) 155, 484
Bse1I ACTGG 1 cut(s) 502
Bse3DI GCAATG 1 cut(s) 295
BseAI TCCGGA 1 cut(s) 187
BseDI CCNNGG 2 cut(s) 116, 225
BseGI GGATG 2 cut(s) 514, 534
BseLI CCNNNNNNNGG 1 cut(s) 117
BseMI GCAATG 1 cut(s) 295
BseMII CTCAG 2 cut(s) 40, 139
BseNI ACTGG 1 cut(s) 502
BseRI GAGGAG 3 cut(s) 5, 296, 507
BseXI GCAGC 5 cut(s) 186, 224, 280, 283, 286
Bsh1236I CGCG 1 cut(s) 118
BshFI GGCC 2 cut(s) 8, 121
BsiSI CCGG 4 cut(s) 156, 188, 218, 485
BslFI GGGAC 1 cut(s) 311
BslI CCNNNNNNNGG 1 cut(s) 117
BsmFI GGGAC 1 cut(s) 311
BsmI GAATGC 1 cut(s) 183
BsnI GGCC 2 cut(s) 8, 121
Bsp13I TCCGGA 1 cut(s) 187
BspACI CCGC 6 cut(s) 105, 116, 118, 235, 259, 303
BspANI GGCC 2 cut(s) 8, 121
BspCNI CTCAG 2 cut(s) 39, 138
BspEI TCCGGA 1 cut(s) 187
BspFNI CGCG 1 cut(s) 118
BspHI TCATGA 1 cut(s) 312
BspLI GGNNCC 2 cut(s) 222, 547
BsrDI GCAATG 1 cut(s) 295
BsrFI RCCGGY 2 cut(s) 155, 484
BsrI ACTGG 1 cut(s) 502
BssAI RCCGGY 2 cut(s) 155, 484
BssECI CCNNGG 2 cut(s) 116, 225
BstBAI YACGTR 1 cut(s) 388
BstDEI CTNAG 2 cut(s) 26, 125
BstDSI CCRYGG 2 cut(s) 116, 225
BstF5I GGATG 2 cut(s) 514, 534
BstFNI CGCG 1 cut(s) 118
BstMWI GCNNNNNNNGC 4 cut(s) 180, 265, 274, 332
BstUI CGCG 1 cut(s) 118
BstV1I GCAGC 5 cut(s) 186, 224, 280, 283, 286
BstXI CCANNNNNNTGG 1 cut(s) 345
BsuRI GGCC 2 cut(s) 8, 121
BtgI CCRYGG 2 cut(s) 116, 225
BtsCI GGATG 2 cut(s) 514, 534
BtsI GCAGTG 1 cut(s) 166
BtsIMutI CAGTG 2 cut(s) 166, 192
CciI TCATGA 1 cut(s) 312
Cfr10I RCCGGY 2 cut(s) 155, 484
Cfr13I GGNCC 2 cut(s) 220, 482
Cfr42I CCGCGG 1 cut(s) 119
CseI GACGC 1 cut(s) 341
CviAII CATG 2 cut(s) 313, 375
DdeI CTNAG 2 cut(s) 26, 125
EciI GGCGGA 2 cut(s) 274, 318
Eco47I GGWCC 2 cut(s) 220, 482
FaeI CATG 2 cut(s) 316, 378
FaiI YATR 6 cut(s) 93, 314, 356, 376, 432, 446
FaqI GGGAC 1 cut(s) 311
FatI CATG 2 cut(s) 312, 374
FauI CCCGC 1 cut(s) 112
Fnu4HI GCNGC 6 cut(s) 119, 175, 213, 269, 272, 275
FokI GGATG 2 cut(s) 521, 521
Fsp4HI GCNGC 6 cut(s) 119, 175, 213, 269, 272, 275
FspBI CTAG 3 cut(s) 140, 180, 327
GluI GCNGC 6 cut(s) 119, 175, 213, 269, 272, 275
HaeIII GGCC 2 cut(s) 8, 121
HapII CCGG 4 cut(s) 156, 188, 218, 485
HgaI GACGC 1 cut(s) 341
Hin1II CATG 2 cut(s) 316, 378
HinfI GANTC 2 cut(s) 129, 463
HpaII CCGG 4 cut(s) 156, 188, 218, 485
HphI GGTGA 1 cut(s) 93
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 1 cut(s) 128
Hpy188III TCNNGA 4 cut(s) 188, 252, 313, 549
Hpy8I GTNNAC 1 cut(s) 145
HpyAV CCTTC 5 cut(s) 81, 249, 310, 364, 391
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 1 cut(s) 512
HpyF10VI GCNNNNNNNGC 4 cut(s) 180, 265, 274, 332
HpyF3I CTNAG 2 cut(s) 26, 125
HpySE526I ACGT 1 cut(s) 387
Hsp92II CATG 2 cut(s) 316, 378
Kpn2I TCCGGA 1 cut(s) 187
KspI CCGCGG 1 cut(s) 119
LmnI GCTCC 2 cut(s) 340, 379
LpnPI CCDG 7 cut(s) 51, 169, 201, 231, 457, 498, 515
Lsp1109I GCAGC 5 cut(s) 186, 224, 280, 283, 286
LweI GCATC 1 cut(s) 499
MaeI CTAG 3 cut(s) 140, 180, 327
MaeII ACGT 1 cut(s) 387
MaeIII GTNAC 1 cut(s) 329
MboII GAAGA 2 cut(s) 88, 362
MluCI AATT 1 cut(s) 438
MmeI TCCRAC 3 cut(s) 101, 460, 486
MroI TCCGGA 1 cut(s) 187
MseI TTAA 2 cut(s) 416, 492
MslI CAYNNNNRTG 1 cut(s) 230
MspA1I CMGCKG 1 cut(s) 118
MspI CCGG 4 cut(s) 156, 188, 218, 485
Mva1269I GAATGC 1 cut(s) 183
MvnI CGCG 1 cut(s) 118
MwoI GCNNNNNNNGC 4 cut(s) 180, 265, 274, 332
NlaIII CATG 2 cut(s) 316, 378
NlaIV GGNNCC 2 cut(s) 222, 547
NmuCI GTSAC 1 cut(s) 329
OliI CACNNNNGTG 1 cut(s) 230
PagI TCATGA 1 cut(s) 312
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PctI GAATGC 1 cut(s) 183
PfeI GAWTC 2 cut(s) 129, 463
PkrI GCNGC 6 cut(s) 120, 176, 214, 270, 273, 276
Ppu21I YACGTR 1 cut(s) 388
PspN4I GGNNCC 2 cut(s) 222, 547
PspPI GGNCC 2 cut(s) 220, 482
RseI CAYNNNNRTG 1 cut(s) 230
SacII CCGCGG 1 cut(s) 119
SaqAI TTAA 2 cut(s) 416, 492
SatI GCNGC 6 cut(s) 119, 175, 213, 269, 272, 275
Sau96I GGNCC 2 cut(s) 220, 482
SfaNI GCATC 1 cut(s) 499
Sfr303I CCGCGG 1 cut(s) 119
SgrBI CCGCGG 1 cut(s) 119
SinI GGWCC 2 cut(s) 220, 482
SmiMI CAYNNNNRTG 1 cut(s) 230
SmlI CTYRAG 2 cut(s) 321, 539
SmoI CTYRAG 2 cut(s) 321, 539
SpeI ACTAGT 1 cut(s) 139
Sse9I AATT 1 cut(s) 438
SsiI CCGC 6 cut(s) 105, 116, 118, 235, 259, 303
SspMI CTAG 3 cut(s) 140, 180, 327
TaiI ACGT 1 cut(s) 390
TaqI TCGA 1 cut(s) 251
TasI AATT 1 cut(s) 438
TauI GCSGC 1 cut(s) 121
TfiI GAWTC 2 cut(s) 129, 463
Tru1I TTAA 2 cut(s) 416, 492
Tru9I TTAA 2 cut(s) 416, 492
TscAI CASTG 2 cut(s) 166, 199
TseFI GTSAC 1 cut(s) 329
TseI GCWGC 5 cut(s) 174, 212, 268, 271, 274
Tsp45I GTSAC 1 cut(s) 329
TspDTI ATGAA 1 cut(s) 329
TspRI CASTG 2 cut(s) 166, 199
VpaK11BI GGWCC 2 cut(s) 220, 482
XspI CTAG 3 cut(s) 140, 180, 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.