Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0015803

Physical Location & Seq

Genomic Coordinates Reverse (-)

11932569 .. 11935461

Gene Structure 2893 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1143700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1311 bp

ATGAAGCATCCATCTTTTGCTCTTCTTTTAGCAATTTTAATCCACCAGCAGTGTTTTATTACGCCAATCCATGTTTCAGCAGCAGGTGATAATTTCATCAGAGTGAGGGGAGTGCATTTTCTTCTCAACGGCAGCCCTTACTATGCAAACGGCTTCAATGGCTACTGGCTCATGTACGTGGCTTCCGACCTGTCTCAGAGGCACAAAGTCTCTTCTGTTTTCAGTGAAGCAACTAGCCACGGGCTCACCGTGGCTCGAACTTGGGCTTTCAGTGATGGAGGTTACAGGCCTCTTCAGCTCTCCCCTGGCTCATACAATGAGCAAATGTTTAAGGGGTTGGATTTTGTTATAGCTGAGGCCAGAAGATATGGGATTAAGCTGATATTAAGCTTGGTGAACAACTATGAGAGCTTTGGAGGAAGAAAGCAGTATGTGAATTGGGCAAGAAGTAAAGGGCAGTACCTCGCATCTGATGACGATTTCTTCAGGAACCCGGTTGTGAAGGGTTACTACAAGAACCATGTAACCACTGTTCTTAACAGATATAACAGCTACACTGGAGTTCATTACAAAGATGACCCAACAATTTTGGCCTGGGAACTTATTAATGAACCTAGATGCACATCAGATCCTTCAGGAAGGACTATTCAGGCATGGATAATGGAAATGGCTTCACACGTGAAGTCAATAGACAGAAACCACTTACTGGAAGCTGGTCTAGAGGGATTTTATGGACAAGCAATACCACATAGGATGAGTCTCAATCCTCCCTTCAATATCGGAACAGACTTTATCGCGAATAATCGGATTCCTGGCATTGATTTTGCAACAGTTCACTCGTATCCTGATCAATGGTTGTCTAGCTCAAATGATCAAACTCAGCTCACTTTCTTGAACAACTGGCTCAACACCCACATCCAAGATGCGCAGAACATTCTCCGAAAGCCAATATTCATCGCAGAGTTTGGGAAATCTTGGAAAGATCCTGGTTTGAGCACCTACCAAAGAGACCTGCTCTTCAACACGGTGTACTCCAAGATATACTTTTCGGCAAAAAGAGGAGGAGCAGCTGCCGGGGGACTATTCTGGCAGCTCCTCACCGAAGGCATGGACTCTTTCCGTGATGGATACGACATAGTTCTGAGTCAGAATCCCTCAACAGAAAATGTGATTGCTCAACAGTCTCACAAGCTCAACCAGATTCGGAAGATTTTTGCGCGGAGAAGAAATGCTTGGATGTGGAGGAGGGCAAGGGCCATTAGAAGGGCTGAATGGTTGGGTAAACACAAAGGCAAACCTATTGGAAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000272 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0004567 GO:0005575 GO:0005576 GO:0005623 GO:0005975 GO:0005976 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009636 GO:0009664 GO:0009791 GO:0009827 GO:0009828 GO:0009845 GO:0009900 GO:0009987 GO:0010035 GO:0010038 GO:0010047 GO:0010154 GO:0010412 GO:0015923 GO:0016043 GO:0016052 GO:0016787 GO:0016798 GO:0016985 GO:0016998 GO:0022414 GO:0032501 GO:0032502 GO:0032504 GO:0042221 GO:0042545 GO:0043170 GO:0044036 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044464 GO:0045229 GO:0046355 GO:0046686 GO:0048046 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0061687 GO:0071554 GO:0071555 GO:0071585 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0090351 GO:0097501 GO:0098754 GO:1901575 GO:1990059 GO:1990170

KEGG Pathways

Metabolic & Signaling

ko00051 map00051

Pfam Domains

Protein Families

Cellulase

Protein Analysis

437

Amino Acids

49.82

Weight (kDa)

9.63

Isoelectric Point (pI)

35.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GH5_mannosidase	PF26410	31 - 364	4.7e-53	GH family 5 beta-mannosidase-like
Cellulase	PF00150	71 - 329	9.2e-11	Cellulase (glycosyl hydrolase family 5)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015803)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G66460
malus_domestica	MD02G1143700.v1.1 MD15G1257000.v1.1
prunus_persica	Prupe.7G158000_v2.0.a1 Prupe.7G158000_v2.0.a1
pyrus_communis	pycom02g11360 pycom15g22490
rosa_chinensis	RchiOBHm_Chr2g0100951
rosa_laevigata	RLG00000017028
rosa_multiflora	Rmu_sc0001862.1_g000014
rosa_samantha	Rh2AG141600 Rh2BG146800 Rh2CG148100 Rh2DG147300
rosa_wichuraiana	Rw2G011110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	74
Acc16I	TGCGCA	1 cut(s)	927
Acc36I	ACCTGC	2 cut(s)	74, 1020
AccB7I	CCANNNNNTGG	1 cut(s)	706
AccII	CGCG	2 cut(s)	797, 1219
AciI	CCGC	1 cut(s)	1219
AclWI	GGATC	2 cut(s)	623, 977
AcuI	CTGAAG	3 cut(s)	278, 469, 618
AcvI	CACGTG	1 cut(s)	679
AfaI	GTAC	3 cut(s)	176, 461, 1031
AfiI	CCNNNNNNNGG	2 cut(s)	706, 1263
AflIII	ACRYGT	1 cut(s)	676
AgsI	TTSAA	4 cut(s)	157, 775, 895, 1021
AjnI	CCWGG	4 cut(s)	304, 593, 811, 985
AjuI	GAANNNNNNNTTGG	4 cut(s)	58, 90, 1216, 1248
Alw21I	GWGCWC	1 cut(s)	998
Alw26I	GTCTC	5 cut(s)	198, 214, 764, 1002, 1188
AlwI	GGATC	2 cut(s)	623, 977
AoxI	GGCC	4 cut(s)	287, 357, 591, 1254
ApeKI	GCWGC	5 cut(s)	80, 132, 1067, 1070, 1090
AseI	ATTAAT	1 cut(s)	606
AspLEI	GCGC	2 cut(s)	928, 1219
AspS9I	GGNCC	1 cut(s)	1254
AsuC2I	CCSGG	2 cut(s)	494, 1075
AsuHPI	GGTGA	4 cut(s)	98, 238, 406, 1090
BanII	GRGCYC	1 cut(s)	246
BarI	GAAGNNNNNNTAC	2 cut(s)	494, 526
BbrPI	CACGTG	1 cut(s)	679
Bbv12I	GWGCWC	1 cut(s)	998
BbvCI	CCTCAGC	1 cut(s)	354
BbvI	GCAGC	5 cut(s)	92, 144, 1057, 1079, 1102
BccI	CCATC	3 cut(s)	19, 269, 1118
BceAI	ACGGC	2 cut(s)	145, 166
BciT130I	CCWGG	4 cut(s)	306, 595, 813, 987
BciVI	GTATCC	2 cut(s)	852, 1121
BclI	TGATCA	2 cut(s)	847, 871
BcnI	CCSGG	2 cut(s)	494, 1075
BcoDI	GTCTC	5 cut(s)	198, 214, 764, 1002, 1188
BfaI	CTAG	4 cut(s)	234, 615, 719, 861
BfuAI	ACCTGC	2 cut(s)	74, 1020
BfuI	GTATCC	2 cut(s)	852, 1121
BisI	GCNGC	5 cut(s)	81, 133, 1068, 1071, 1091
BlsI	GCNGC	5 cut(s)	82, 134, 1069, 1072, 1092
Bme1390I	CCNGG	6 cut(s)	306, 494, 595, 813, 987, 1075
BmgT120I	GGNCC	1 cut(s)	1254
BmiI	GGNNCC	1 cut(s)	491
BmrFI	CCNGG	6 cut(s)	306, 494, 595, 813, 987, 1075
BmsI	GCATC	4 cut(s)	16, 476, 608, 913
BplI	GAGNNNNNCTC	2 cut(s)	999, 1031
BpmI	CTGGAG	1 cut(s)	579
Bpu10I	CCTNAGC	1 cut(s)	354
BpuMI	CCSGG	2 cut(s)	494, 1075
BsaAI	YACGTR	2 cut(s)	178, 679
BsaBI	GATNNNNATC	1 cut(s)	622
BsaI	GGTCTC	1 cut(s)	1002
BsaJI	CCNNGG	5 cut(s)	238, 249, 304, 594, 1074
Bsc4I	CCNNNNNNNGG	2 cut(s)	706, 1263
Bse1I	ACTGG	4 cut(s)	170, 562, 711, 905
Bse8I	GATNNNNATC	1 cut(s)	622
BseBI	CCWGG	4 cut(s)	306, 595, 813, 987
BseDI	CCNNGG	5 cut(s)	238, 249, 304, 594, 1074
BseGI	GGATG	4 cut(s)	7, 759, 915, 1242
BseJI	GATNNNNATC	1 cut(s)	622
BseLI	CCNNNNNNNGG	2 cut(s)	706, 1263
BseMII	CTCAG	4 cut(s)	209, 345, 893, 1133
BseNI	ACTGG	4 cut(s)	170, 562, 711, 905
BseRI	GAGGAG	4 cut(s)	1074, 1077, 1085, 1258
BseXI	GCAGC	5 cut(s)	92, 144, 1057, 1079, 1102
Bsh1236I	CGCG	2 cut(s)	797, 1219
BshFI	GGCC	4 cut(s)	289, 359, 593, 1256
BsiHKAI	GWGCWC	1 cut(s)	998
BsiSI	CCGG	2 cut(s)	494, 1074
BslFI	GGGAC	1 cut(s)	1092
BslI	CCNNNNNNNGG	2 cut(s)	706, 1263
BsmAI	GTCTC	5 cut(s)	198, 214, 764, 1002, 1188
BsmFI	GGGAC	1 cut(s)	1092
BsnI	GGCC	4 cut(s)	289, 359, 593, 1256
Bso31I	GGTCTC	1 cut(s)	1002
Bsp1286I	GDGCHC	2 cut(s)	246, 998
Bsp143I	GATC	4 cut(s)	628, 847, 871, 982
Bsp68I	TCGCGA	1 cut(s)	797
BspACI	CCGC	1 cut(s)	1219
BspANI	GGCC	4 cut(s)	289, 359, 593, 1256
BspCNI	CTCAG	4 cut(s)	208, 346, 892, 1134
BspFNI	CGCG	2 cut(s)	797, 1219
BspLI	GGNNCC	1 cut(s)	491
BspMI	ACCTGC	2 cut(s)	74, 1020
BspPI	GGATC	2 cut(s)	623, 977
BspQI	GCTCTTC	2 cut(s)	27, 1022
BspTNI	GGTCTC	1 cut(s)	1002
BsrI	ACTGG	4 cut(s)	170, 562, 711, 905
BssECI	CCNNGG	5 cut(s)	238, 249, 304, 594, 1074
BssMI	GATC	4 cut(s)	628, 847, 871, 982
Bst2UI	CCWGG	4 cut(s)	306, 595, 813, 987
Bst4CI	ACNGT	5 cut(s)	250, 532, 832, 1027, 1182
Bst6I	CTCTTC	4 cut(s)	27, 217, 297, 1022
BstBAI	YACGTR	2 cut(s)	178, 679
BstDEI	CTNAG	4 cut(s)	195, 354, 879, 1142
BstDSI	CCRYGG	2 cut(s)	238, 249
BstF5I	GGATG	4 cut(s)	7, 759, 915, 1242
BstFNI	CGCG	2 cut(s)	797, 1219
BstHHI	GCGC	2 cut(s)	928, 1219
BstKTI	GATC	4 cut(s)	631, 850, 874, 985
BstMAI	GTCTC	5 cut(s)	198, 214, 764, 1002, 1188
BstMBI	GATC	4 cut(s)	628, 847, 871, 982
BstMWI	GCNNNNNNNGC	2 cut(s)	159, 295
BstNI	CCWGG	4 cut(s)	306, 595, 813, 987
BstSCI	CCNGG	6 cut(s)	304, 492, 593, 811, 985, 1073
BstUI	CGCG	2 cut(s)	797, 1219
BstV1I	GCAGC	5 cut(s)	92, 144, 1057, 1079, 1102
BstX2I	RGATCY	2 cut(s)	628, 982
BstYI	RGATCY	2 cut(s)	628, 982
BsuI	GTATCC	2 cut(s)	852, 1121
BsuRI	GGCC	4 cut(s)	289, 359, 593, 1256
BtgI	CCRYGG	2 cut(s)	238, 249
BtgZI	GCGATG	1 cut(s)	940
BtsCI	GGATG	4 cut(s)	7, 759, 915, 1242
BtsI	GCAGTG	1 cut(s)	56
BtsIMutI	CAGTG	5 cut(s)	56, 229, 277, 528, 555
BtuMI	TCGCGA	1 cut(s)	797
BveI	ACCTGC	2 cut(s)	74, 1020
CfoI	GCGC	2 cut(s)	928, 1219
Cfr13I	GGNCC	1 cut(s)	1254
Csp6I	GTAC	3 cut(s)	175, 460, 1030
CviAII	CATG	5 cut(s)	71, 172, 521, 654, 1108
CviQI	GTAC	3 cut(s)	175, 460, 1030
DdeI	CTNAG	4 cut(s)	195, 354, 879, 1142
DpnI	GATC	4 cut(s)	630, 849, 873, 984
DpnII	GATC	4 cut(s)	628, 847, 871, 982
Eam1104I	CTCTTC	4 cut(s)	27, 217, 297, 1022
EarI	CTCTTC	4 cut(s)	27, 217, 297, 1022
Eco147I	AGGCCT	1 cut(s)	289
Eco24I	GRGCYC	1 cut(s)	246
Eco31I	GGTCTC	1 cut(s)	1002
Eco57I	CTGAAG	3 cut(s)	278, 469, 618
Eco72I	CACGTG	1 cut(s)	679
EcoRII	CCWGG	4 cut(s)	304, 593, 811, 985
EcoT38I	GRGCYC	1 cut(s)	246
FaeI	CATG	5 cut(s)	74, 175, 524, 657, 1111
FalI	AAGNNNNNCTT	4 cut(s)	1028, 1060, 1216, 1248
FaqI	GGGAC	1 cut(s)	1092
FatI	CATG	5 cut(s)	70, 171, 520, 653, 1107
FbaI	TGATCA	2 cut(s)	847, 871
Fnu4HI	GCNGC	5 cut(s)	81, 133, 1068, 1071, 1091
FokI	GGATG	3 cut(s)	766, 902, 1249
FriOI	GRGCYC	1 cut(s)	246
Fsp4HI	GCNGC	5 cut(s)	81, 133, 1068, 1071, 1091
FspBI	CTAG	4 cut(s)	234, 615, 719, 861
FspI	TGCGCA	1 cut(s)	927
GlaI	GCGC	2 cut(s)	927, 1218
GluI	GCNGC	5 cut(s)	81, 133, 1068, 1071, 1091
GsuI	CTGGAG	1 cut(s)	579
HaeIII	GGCC	4 cut(s)	289, 359, 593, 1256
HapII	CCGG	2 cut(s)	494, 1074
HhaI	GCGC	2 cut(s)	928, 1219
Hin1II	CATG	5 cut(s)	74, 175, 524, 657, 1111
Hin6I	GCGC	2 cut(s)	926, 1217
HinP1I	GCGC	2 cut(s)	926, 1217
HindIII	AAGCTT	1 cut(s)	388
HinfI	GANTC	6 cut(s)	757, 808, 1112, 1144, 1150, 1201
HpaII	CCGG	2 cut(s)	494, 1074
HphI	GGTGA	4 cut(s)	98, 238, 406, 1090
Hpy166II	GTNNAC	4 cut(s)	397, 835, 1030, 1283
Hpy188III	TCNNGA	6 cut(s)	487, 636, 719, 796, 845, 892
Hpy8I	GTNNAC	4 cut(s)	397, 835, 1030, 1283
HpyAV	CCTTC	6 cut(s)	496, 633, 642, 781, 1097, 1257
HpyCH4III	ACNGT	5 cut(s)	250, 532, 832, 1027, 1182
HpyCH4IV	ACGT	2 cut(s)	177, 678
HpyCH4V	TGCA	4 cut(s)	115, 146, 621, 827
HpyF10VI	GCNNNNNNNGC	2 cut(s)	159, 295
HpyF3I	CTNAG	4 cut(s)	195, 354, 879, 1142
HpySE526I	ACGT	2 cut(s)	177, 678
Hsp92II	CATG	5 cut(s)	74, 175, 524, 657, 1111
HspAI	GCGC	2 cut(s)	926, 1217
Ksp22I	TGATCA	2 cut(s)	847, 871
Kzo9I	GATC	4 cut(s)	628, 847, 871, 982
LguI	GCTCTTC	2 cut(s)	27, 1022
LmnI	GCTCC	2 cut(s)	1064, 1098
Lsp1109I	GCAGC	5 cut(s)	92, 144, 1057, 1079, 1102
LweI	GCATC	4 cut(s)	16, 476, 608, 913
MaeI	CTAG	4 cut(s)	234, 615, 719, 861
MaeII	ACGT	2 cut(s)	177, 678
MaeIII	GTNAC	3 cut(s)	281, 506, 523
MalI	GATC	4 cut(s)	630, 849, 873, 984
MboI	GATC	4 cut(s)	628, 847, 871, 982
MflI	RGATCY	2 cut(s)	628, 982
MhlI	GDGCHC	2 cut(s)	246, 998
MluCI	AATT	5 cut(s)	33, 91, 436, 585, 1306
MlyI	GAGTC	3 cut(s)	766, 1106, 1153
MmeI	TCCRAC	2 cut(s)	210, 318
MseI	TTAA	6 cut(s)	38, 330, 375, 386, 537, 606
MslI	CAYNNNNRTG	2 cut(s)	101, 176
MspA1I	CMGCKG	1 cut(s)	1070
MspI	CCGG	2 cut(s)	494, 1074
MspR9I	CCNGG	6 cut(s)	306, 494, 595, 813, 987, 1075
MvaI	CCWGG	4 cut(s)	306, 595, 813, 987
MvnI	CGCG	2 cut(s)	797, 1219
MwoI	GCNNNNNNNGC	2 cut(s)	159, 295
NciI	CCSGG	2 cut(s)	494, 1075
NdeII	GATC	4 cut(s)	628, 847, 871, 982
NlaIII	CATG	5 cut(s)	74, 175, 524, 657, 1111
NlaIV	GGNNCC	1 cut(s)	491
NruI	TCGCGA	1 cut(s)	797
NsbI	TGCGCA	1 cut(s)	927
PaqCI	CACCTGC	1 cut(s)	74
PceI	AGGCCT	1 cut(s)	289
PciSI	GCTCTTC	2 cut(s)	27, 1022
PcsI	WCGNNNNNNNCGW	2 cut(s)	183, 246
PfeI	GAWTC	3 cut(s)	808, 1150, 1201
PflMI	CCANNNNNTGG	1 cut(s)	706
PkrI	GCNGC	5 cut(s)	82, 134, 1069, 1072, 1092
PleI	GAGTC	3 cut(s)	765, 1106, 1152
PmaCI	CACGTG	1 cut(s)	679
PmlI	CACGTG	1 cut(s)	679
PpsI	GAGTC	3 cut(s)	765, 1106, 1152
Ppu21I	YACGTR	2 cut(s)	178, 679
PshBI	ATTAAT	1 cut(s)	606
Psp6I	CCWGG	4 cut(s)	304, 593, 811, 985
PspCI	CACGTG	1 cut(s)	679
PspGI	CCWGG	4 cut(s)	304, 593, 811, 985
PspN4I	GGNNCC	1 cut(s)	491
PspPI	GGNCC	1 cut(s)	1254
PsrI	GAACNNNNNNTAC	2 cut(s)	516, 548
PsuI	RGATCY	2 cut(s)	628, 982
PvuII	CAGCTG	1 cut(s)	1070
RruI	TCGCGA	1 cut(s)	797
RsaI	GTAC	3 cut(s)	176, 461, 1031
RsaNI	GTAC	3 cut(s)	175, 460, 1030
RseI	CAYNNNNRTG	2 cut(s)	101, 176
SapI	GCTCTTC	2 cut(s)	27, 1022
SaqAI	TTAA	6 cut(s)	38, 330, 375, 386, 537, 606
SatI	GCNGC	5 cut(s)	81, 133, 1068, 1071, 1091
Sau3AI	GATC	4 cut(s)	628, 847, 871, 982
Sau96I	GGNCC	1 cut(s)	1254
SchI	GAGTC	3 cut(s)	766, 1106, 1153
ScrFI	CCNGG	6 cut(s)	306, 494, 595, 813, 987, 1075
SduI	GDGCHC	2 cut(s)	246, 998
SfaNI	GCATC	4 cut(s)	16, 476, 608, 913
SmiMI	CAYNNNNRTG	2 cut(s)	101, 176
Sse9I	AATT	5 cut(s)	33, 91, 436, 585, 1306
SseBI	AGGCCT	1 cut(s)	289
SsiI	CCGC	1 cut(s)	1219
SspI	AATATT	1 cut(s)	951
SspMI	CTAG	4 cut(s)	234, 615, 719, 861
StuI	AGGCCT	1 cut(s)	289
StyD4I	CCNGG	6 cut(s)	304, 492, 593, 811, 985, 1073
TaaI	ACNGT	5 cut(s)	250, 532, 832, 1027, 1182
TaiI	ACGT	2 cut(s)	180, 681
TaqI	TCGA	1 cut(s)	256
TasI	AATT	5 cut(s)	33, 91, 436, 585, 1306
TatI	WGTACW	1 cut(s)	1029
TfiI	GAWTC	3 cut(s)	808, 1150, 1201
Tru1I	TTAA	6 cut(s)	38, 330, 375, 386, 537, 606
Tru9I	TTAA	6 cut(s)	38, 330, 375, 386, 537, 606
TscAI	CASTG	5 cut(s)	56, 229, 277, 535, 562
TseI	GCWGC	5 cut(s)	80, 132, 1067, 1070, 1090
TspDTI	ATGAA	5 cut(s)	17, 85, 554, 624, 943
TspGWI	ACGGA	1 cut(s)	1109
TspRI	CASTG	5 cut(s)	56, 229, 277, 535, 562
Van91I	CCANNNNNTGG	1 cut(s)	706
VspI	ATTAAT	1 cut(s)	606
XbaI	TCTAGA	1 cut(s)	718
XspI	CTAG	4 cut(s)	234, 615, 719, 861

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.