Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0015803

Physical Location & Seq

Genomic Coordinates Reverse (-)

21870153 .. 21872388

Gene Structure 2236 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1257000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1086 bp

ATGTGGCTTCTGACCCATCTCGGAGGCACAAAGTCTCGTCTGCTTTCCGCGAAGCAACTAGACGGACTCACCGTGGCTAGAACTTGGGCTTTCAGTGATGGAGGTTACATGCCTCTTCAGTTCTCCCCTGGCTCCTACAACGAGCACATGTTTAAGGGGTTGGATTTTGTGATAGCTGAGGCCAGAAGGTATGAGATTAAGATGATATTAAGCTTGGTGAACAACTATGAGAACTTTGGAGGAAGAAAGCAGTATGTGAATTGGGCAAGAAGGAAAGGGCAGTACCTCACATCTGATGATGATTTCTTCAGGAACCCTGTTGTGAAGGGTTACTACAAGAACCATGTAACTACTGTTGTTAACAGATATAACAGCTACATTGGAGTTCATTACAAAGATGACCCAACAATTATGGCATGGGAACTTATTAATGAGCCTAGATGCTCATCAGATCCTTCAGGAAGGACTATTCAGGCTTGGATAAAGGAAATGGCTTCACATGTGAAGTCAATAGATAGAAACCACTTATTGGAAGTTGGTCTAGAGGGATTTTATGGACAAGAAATACCTCAGAGGATGAGTCTCAATCCTGGTTATAATATTGGAACAGAATTTATCGCGAATAATAGGATTCCTGGGATCGATTTCACAACAATTCATTCTTATCCTGATCAATGGTTGGGTAGCTCTAATGATCAACATCAGGTCGTTTTCTCGAACAACTGGCTCAACACCCACATCCAAGTTGCACAGAACATTCTTCAAAAGCCGATATACATTGCAGAGTTTGGGAAGTCTTGGAAAGATCTCGGTTTCAGAACTTACCAAAGAGACTTGCTCTTCAATACCGTGTACTCCAAGATATACTTTTTGGCGAAAAGAGGAGGAGCAGCTGCTGGGGGACTATTCTGGCAACTTCTCACTGAAGGCATGGACTCTTTCCGTGATGGATACGACATAGTTCTGAGTCAGAGTCCCTCAACAGCAAATGTGATTGCTCAACAGTCTCACAAGCTCAATCAGATTCGGAAGATCTTTTGCGCGGATAAGAAATGCTCGGATGTGGAAGAGGGCAAGGGCCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000272 GO:0003006 GO:0003674 GO:0003824 GO:0004553 GO:0004567 GO:0005575 GO:0005576 GO:0005623 GO:0005975 GO:0005976 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009636 GO:0009664 GO:0009791 GO:0009827 GO:0009828 GO:0009845 GO:0009900 GO:0009987 GO:0010035 GO:0010038 GO:0010047 GO:0010154 GO:0010412 GO:0015923 GO:0016043 GO:0016052 GO:0016787 GO:0016798 GO:0016985 GO:0016998 GO:0022414 GO:0032501 GO:0032502 GO:0032504 GO:0042221 GO:0042545 GO:0043170 GO:0044036 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044464 GO:0045229 GO:0046355 GO:0046686 GO:0048046 GO:0048608 GO:0048609 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0061687 GO:0071554 GO:0071555 GO:0071585 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0090351 GO:0097501 GO:0098754 GO:1901575 GO:1990059 GO:1990170

KEGG Pathways

Metabolic & Signaling

ko00051 map00051

Pfam Domains

Protein Families

Cellulase

Protein Analysis

362

Amino Acids

41.36

Weight (kDa)

8.87

Isoelectric Point (pI)

35.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cellulase	PF00150	28 - 270	7.6e-10	Cellulase (glycosyl hydrolase family 5)
GH5_mannosidase	PF26410	37 - 305	6.7e-50	GH family 5 beta-mannosidase-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015803)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G66460
malus_domestica	MD02G1143700.v1.1 MD15G1257000.v1.1
prunus_persica	Prupe.7G158000_v2.0.a1 Prupe.7G158000_v2.0.a1
pyrus_communis	pycom02g11360 pycom15g22490
rosa_chinensis	RchiOBHm_Chr2g0100951
rosa_laevigata	RLG00000017028
rosa_multiflora	Rmu_sc0001862.1_g000014
rosa_samantha	Rh2AG141600 Rh2BG146800 Rh2CG148100 Rh2DG147300
rosa_wichuraiana	Rw2G011110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	597
AccB7I	CCANNNNNTGG	1 cut(s)	529
AccII	CGCG	3 cut(s)	50, 620, 1043
AciI	CCGC	2 cut(s)	48, 1043
AclWI	GGATC	2 cut(s)	446, 647
AcsI	RAATTY	1 cut(s)	611
AcuI	CTGAAG	4 cut(s)	101, 292, 441, 945
AfaI	GTAC	2 cut(s)	284, 854
AfiI	CCNNNNNNNGG	1 cut(s)	529
AflIII	ACRYGT	2 cut(s)	147, 499
AgsI	TTSAA	2 cut(s)	764, 844
AjnI	CCWGG	3 cut(s)	127, 589, 634
AluBI	AGCT	6 cut(s)	176, 213, 375, 687, 893, 1015
AluI	AGCT	6 cut(s)	176, 213, 375, 687, 893, 1015
Alw21I	GWGCWC	1 cut(s)	147
Alw26I	GTCTC	4 cut(s)	39, 587, 825, 1011
AlwI	GGATC	2 cut(s)	446, 647
AlwNI	CAGNNNCTG	1 cut(s)	896
AoxI	GGCC	2 cut(s)	180, 1078
ApeKI	GCWGC	2 cut(s)	890, 893
ApoI	RAATTY	1 cut(s)	611
AseI	ATTAAT	1 cut(s)	429
AspLEI	GCGC	1 cut(s)	1043
AspS9I	GGNCC	1 cut(s)	1078
AsuHPI	GGTGA	2 cut(s)	61, 229
BarI	GAAGNNNNNNTAC	2 cut(s)	317, 349
Bbv12I	GWGCWC	1 cut(s)	147
BbvCI	CCTCAGC	1 cut(s)	177
BbvI	GCAGC	2 cut(s)	880, 902
BccI	CCATC	3 cut(s)	24, 92, 941
BciT130I	CCWGG	3 cut(s)	129, 591, 636
BciVI	GTATCC	1 cut(s)	944
BclI	TGATCA	2 cut(s)	670, 694
BcoDI	GTCTC	4 cut(s)	39, 587, 825, 1011
BfaI	CTAG	4 cut(s)	59, 78, 438, 542
BfuI	GTATCC	1 cut(s)	944
BglII	AGATCT	2 cut(s)	805, 1032
BisI	GCNGC	2 cut(s)	891, 894
BlsI	GCNGC	2 cut(s)	892, 895
Bme1390I	CCNGG	3 cut(s)	129, 591, 636
BmgT120I	GGNCC	1 cut(s)	1078
BmiI	GGNNCC	2 cut(s)	133, 314
BmrFI	CCNGG	3 cut(s)	129, 591, 636
BmsI	GCATC	1 cut(s)	431
BplI	GAGNNNNNCTC	2 cut(s)	822, 854
Bpu10I	CCTNAGC	1 cut(s)	177
Bsa29I	ATCGAT	1 cut(s)	642
BsaBI	GATNNNNATC	2 cut(s)	445, 699
BsaJI	CCNNGG	3 cut(s)	72, 127, 635
Bsc4I	CCNNNNNNNGG	1 cut(s)	529
Bse1I	ACTGG	1 cut(s)	728
Bse3DI	GCAATG	1 cut(s)	777
Bse8I	GATNNNNATC	2 cut(s)	445, 699
BseBI	CCWGG	3 cut(s)	129, 591, 636
BseCI	ATCGAT	1 cut(s)	642
BseDI	CCNNGG	3 cut(s)	72, 127, 635
BseGI	GGATG	3 cut(s)	582, 738, 1066
BseJI	GATNNNNATC	2 cut(s)	445, 699
BseLI	CCNNNNNNNGG	1 cut(s)	529
BseMI	GCAATG	1 cut(s)	777
BseMII	CTCAG	3 cut(s)	168, 584, 956
BseNI	ACTGG	1 cut(s)	728
BseRI	GAGGAG	2 cut(s)	897, 900
BseXI	GCAGC	2 cut(s)	880, 902
BseYI	CCCAGC	1 cut(s)	896
Bsh1236I	CGCG	3 cut(s)	50, 620, 1043
BshFI	GGCC	2 cut(s)	182, 1080
BshVI	ATCGAT	1 cut(s)	642
BsiHKAI	GWGCWC	1 cut(s)	147
BslFI	GGGAC	2 cut(s)	915, 960
BslI	CCNNNNNNNGG	1 cut(s)	529
BsmAI	GTCTC	4 cut(s)	39, 587, 825, 1011
BsmFI	GGGAC	2 cut(s)	915, 960
BsnI	GGCC	2 cut(s)	182, 1080
Bsp1286I	GDGCHC	1 cut(s)	147
Bsp143I	GATC	6 cut(s)	451, 639, 670, 694, 805, 1032
Bsp68I	TCGCGA	1 cut(s)	620
BspACI	CCGC	2 cut(s)	48, 1043
BspANI	GGCC	2 cut(s)	182, 1080
BspCNI	CTCAG	3 cut(s)	169, 583, 957
BspDI	ATCGAT	1 cut(s)	642
BspFNI	CGCG	3 cut(s)	50, 620, 1043
BspLI	GGNNCC	2 cut(s)	133, 314
BspPI	GGATC	2 cut(s)	446, 647
BspQI	GCTCTTC	1 cut(s)	845
BsrDI	GCAATG	1 cut(s)	777
BsrI	ACTGG	1 cut(s)	728
BssECI	CCNNGG	3 cut(s)	72, 127, 635
BssMI	GATC	6 cut(s)	451, 639, 670, 694, 805, 1032
Bst2UI	CCWGG	3 cut(s)	129, 591, 636
Bst4CI	ACNGT	4 cut(s)	73, 355, 850, 1005
Bst6I	CTCTTC	3 cut(s)	120, 845, 1062
BstDEI	CTNAG	3 cut(s)	177, 570, 965
BstDSI	CCRYGG	1 cut(s)	72
BstF5I	GGATG	3 cut(s)	582, 738, 1066
BstFNI	CGCG	3 cut(s)	50, 620, 1043
BstHHI	GCGC	1 cut(s)	1043
BstKTI	GATC	6 cut(s)	454, 642, 673, 697, 808, 1035
BstMAI	GTCTC	4 cut(s)	39, 587, 825, 1011
BstMBI	GATC	6 cut(s)	451, 639, 670, 694, 805, 1032
BstNI	CCWGG	3 cut(s)	129, 591, 636
BstNSI	RCATGY	3 cut(s)	112, 151, 503
BstSCI	CCNGG	3 cut(s)	127, 589, 634
BstUI	CGCG	3 cut(s)	50, 620, 1043
BstV1I	GCAGC	2 cut(s)	880, 902
BstX2I	RGATCY	3 cut(s)	451, 805, 1032
BstYI	RGATCY	3 cut(s)	451, 805, 1032
Bsu15I	ATCGAT	1 cut(s)	642
BsuI	GTATCC	1 cut(s)	944
BsuRI	GGCC	2 cut(s)	182, 1080
BsuTUI	ATCGAT	1 cut(s)	642
BtgI	CCRYGG	1 cut(s)	72
BtsCI	GGATG	3 cut(s)	582, 738, 1066
BtsIMutI	CAGTG	2 cut(s)	100, 921
BtuMI	TCGCGA	1 cut(s)	620
CaiI	CAGNNNCTG	1 cut(s)	896
CfoI	GCGC	1 cut(s)	1043
Cfr13I	GGNCC	1 cut(s)	1078
ClaI	ATCGAT	1 cut(s)	642
Csp6I	GTAC	2 cut(s)	283, 853
CviAII	CATG	6 cut(s)	109, 148, 344, 417, 500, 931
CviQI	GTAC	2 cut(s)	283, 853
DdeI	CTNAG	3 cut(s)	177, 570, 965
DpnI	GATC	6 cut(s)	453, 641, 672, 696, 807, 1034
DpnII	GATC	6 cut(s)	451, 639, 670, 694, 805, 1032
Eam1104I	CTCTTC	3 cut(s)	120, 845, 1062
EarI	CTCTTC	3 cut(s)	120, 845, 1062
Eco57I	CTGAAG	4 cut(s)	101, 292, 441, 945
EcoRII	CCWGG	3 cut(s)	127, 589, 634
FaeI	CATG	6 cut(s)	112, 151, 347, 420, 503, 934
FalI	AAGNNNNNCTT	2 cut(s)	851, 883
FaqI	GGGAC	2 cut(s)	915, 960
FatI	CATG	6 cut(s)	108, 147, 343, 416, 499, 930
FbaI	TGATCA	2 cut(s)	670, 694
Fnu4HI	GCNGC	2 cut(s)	891, 894
FokI	GGATG	3 cut(s)	589, 725, 1073
Fsp4HI	GCNGC	2 cut(s)	891, 894
FspBI	CTAG	4 cut(s)	59, 78, 438, 542
GlaI	GCGC	1 cut(s)	1042
GluI	GCNGC	2 cut(s)	891, 894
GsaI	CCCAGC	1 cut(s)	900
HaeIII	GGCC	2 cut(s)	182, 1080
HhaI	GCGC	1 cut(s)	1043
Hin1II	CATG	6 cut(s)	112, 151, 347, 420, 503, 934
Hin6I	GCGC	1 cut(s)	1041
HinP1I	GCGC	1 cut(s)	1041
HincII	GTYRAC	1 cut(s)	361
HindII	GTYRAC	1 cut(s)	361
HindIII	AAGCTT	1 cut(s)	211
HinfI	GANTC	7 cut(s)	66, 580, 631, 935, 967, 973, 1024
HpaI	GTTAAC	1 cut(s)	361
HphI	GGTGA	2 cut(s)	61, 229
Hpy166II	GTNNAC	3 cut(s)	220, 361, 853
Hpy188III	TCNNGA	6 cut(s)	310, 459, 542, 619, 668, 715
Hpy8I	GTNNAC	3 cut(s)	220, 361, 853
HpyAV	CCTTC	6 cut(s)	180, 264, 319, 456, 465, 920
HpyCH4III	ACNGT	4 cut(s)	73, 355, 850, 1005
HpyCH4V	TGCA	2 cut(s)	749, 782
HpyF3I	CTNAG	3 cut(s)	177, 570, 965
Hsp92II	CATG	6 cut(s)	112, 151, 347, 420, 503, 934
HspAI	GCGC	1 cut(s)	1041
Ksp22I	TGATCA	2 cut(s)	670, 694
KspAI	GTTAAC	1 cut(s)	361
Kzo9I	GATC	6 cut(s)	451, 639, 670, 694, 805, 1032
LguI	GCTCTTC	1 cut(s)	845
LmnI	GCTCC	2 cut(s)	137, 887
Lsp1109I	GCAGC	2 cut(s)	880, 902
LweI	GCATC	1 cut(s)	431
MaeI	CTAG	4 cut(s)	59, 78, 438, 542
MaeIII	GTNAC	3 cut(s)	104, 329, 346
MalI	GATC	6 cut(s)	453, 641, 672, 696, 807, 1034
MboI	GATC	6 cut(s)	451, 639, 670, 694, 805, 1032
MboII	GAAGA	7 cut(s)	107, 255, 298, 752, 832, 1042, 1079
MflI	RGATCY	3 cut(s)	451, 805, 1032
MhlI	GDGCHC	1 cut(s)	147
MluCI	AATT	4 cut(s)	259, 408, 611, 654
MlyI	GAGTC	5 cut(s)	60, 589, 929, 976, 982
MmeI	TCCRAC	1 cut(s)	141
MseI	TTAA	5 cut(s)	153, 198, 209, 360, 429
MspA1I	CMGCKG	1 cut(s)	893
MspR9I	CCNGG	3 cut(s)	129, 591, 636
MvaI	CCWGG	3 cut(s)	129, 591, 636
MvnI	CGCG	3 cut(s)	50, 620, 1043
NdeII	GATC	6 cut(s)	451, 639, 670, 694, 805, 1032
NlaIII	CATG	6 cut(s)	112, 151, 347, 420, 503, 934
NlaIV	GGNNCC	2 cut(s)	133, 314
NruI	TCGCGA	1 cut(s)	620
NspI	RCATGY	3 cut(s)	112, 151, 503
PciI	ACATGT	2 cut(s)	147, 499
PciSI	GCTCTTC	1 cut(s)	845
PcsI	WCGNNNNNNNCGW	1 cut(s)	69
PfeI	GAWTC	2 cut(s)	631, 1024
PflMI	CCANNNNNTGG	1 cut(s)	529
PkrI	GCNGC	2 cut(s)	892, 895
PleI	GAGTC	5 cut(s)	60, 588, 929, 975, 981
PpsI	GAGTC	5 cut(s)	60, 588, 929, 975, 981
PscI	ACATGT	2 cut(s)	147, 499
PshBI	ATTAAT	1 cut(s)	429
PsiI	TTATAA	1 cut(s)	597
Psp6I	CCWGG	3 cut(s)	127, 589, 634
PspFI	CCCAGC	1 cut(s)	896
PspGI	CCWGG	3 cut(s)	127, 589, 634
PspN4I	GGNNCC	2 cut(s)	133, 314
PspPI	GGNCC	1 cut(s)	1078
PstNI	CAGNNNCTG	1 cut(s)	896
PsuI	RGATCY	3 cut(s)	451, 805, 1032
PvuII	CAGCTG	1 cut(s)	893
RruI	TCGCGA	1 cut(s)	620
RsaI	GTAC	2 cut(s)	284, 854
RsaNI	GTAC	2 cut(s)	283, 853
SapI	GCTCTTC	1 cut(s)	845
SaqAI	TTAA	5 cut(s)	153, 198, 209, 360, 429
SatI	GCNGC	2 cut(s)	891, 894
Sau3AI	GATC	6 cut(s)	451, 639, 670, 694, 805, 1032
Sau96I	GGNCC	1 cut(s)	1078
SchI	GAGTC	5 cut(s)	60, 589, 929, 976, 982
ScrFI	CCNGG	3 cut(s)	129, 591, 636
SduI	GDGCHC	1 cut(s)	147
SfaNI	GCATC	1 cut(s)	431
Sse9I	AATT	4 cut(s)	259, 408, 611, 654
SsiI	CCGC	2 cut(s)	48, 1043
SspI	AATATT	1 cut(s)	601
SspMI	CTAG	4 cut(s)	59, 78, 438, 542
StyD4I	CCNGG	3 cut(s)	127, 589, 634
TaaI	ACNGT	4 cut(s)	73, 355, 850, 1005
TaqI	TCGA	2 cut(s)	642, 716
TasI	AATT	4 cut(s)	259, 408, 611, 654
TatI	WGTACW	1 cut(s)	852
TfiI	GAWTC	2 cut(s)	631, 1024
Tru1I	TTAA	5 cut(s)	153, 198, 209, 360, 429
Tru9I	TTAA	5 cut(s)	153, 198, 209, 360, 429
TscAI	CASTG	2 cut(s)	100, 928
TseI	GCWGC	2 cut(s)	890, 893
TspDTI	ATGAA	2 cut(s)	377, 647
TspGWI	ACGGA	2 cut(s)	78, 932
TspRI	CASTG	2 cut(s)	100, 928
Van91I	CCANNNNNTGG	1 cut(s)	529
VspI	ATTAAT	1 cut(s)	429
XapI	RAATTY	1 cut(s)	611
XbaI	TCTAGA	1 cut(s)	541
XceI	RCATGY	3 cut(s)	112, 151, 503
XspI	CTAG	4 cut(s)	59, 78, 438, 542

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.