MD02G1150300.v1.1

F-Box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013590
Physical Location & Seq
Reverse (-)
12353776 .. 12356096
2321 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1150300.v1.1.491

Sequence Viewer

Length: 1104 bp
ATGAATCAAGGAAGGAGAGCAAATAAGTTGGTAGGAAACAAAAACAGTATTTTATACATGGACCTCAAAGACATCGTTAGGAACCATGCCCTCCAGTTTCTACCTGCTAAATCACTTTTCAGGTTCACTGGAGTTTGTAGGGACTGGAAGCTCGATATCACAACTCCTTTCTTTGCCCACAAGCAGTCAAATAGCTTTCGTGATATCTCGGGCTTCTTTTTTCAATCCGGAACAGCCCCACCTTCATTCATATCTCTTGACCCCAAGGCGTATGGTGTTCCAGACCCATCTTTGAGCTTCCTGCCTGAGCCGGTTGAGATAAGGAGTTCTTCCAATGGATTACTTTGCTGCCAGGCTCATGATGGCTGTAAGGCTTACTACATTTGCAATCCTGTTACTAGGAAGTGGAAAAAACTTCCCAAGCCAAATGCTGATCACGGATCTAACCCTGCTATTGTTCTCATCTTTGAACCATCTTTGCTCAATTTTGTGGCCGAGTACAAGCTCATATGTGTTTTTCCATCTGTTGATTTTGACAATGGGTATGAGTTTGAGATTTATTCCTCCAGGGATGAGACTTGGAGAGTTTCTGGTGAGATTTACTTTGGCCATCAACATCTCGTGCCAAGAACTGGTGTTCATGTGAACGACATAGTTTATTGGGTATTGGATTATCAGATTCTTGTTTTTGACCTGACGATGGAGCGAGCACAACAACTTTCTGGCAATTTTTATGGAACTGGAGGAGTTGGGGCCCTGGGTCTGATGGATGGAAAGCTTTGTCTGACAACGCCTAGTCCTACAGGACTAACGGTGAAAGTGTTGGCTAACGCCTACACGAATACTATGGGGATGCACAGCAATGCCAGGGCATGGGAAACGAAACATCAGATCAGTCTTTCACCTCCACCGTTCAGGGCATCGGATTATAACCCTCGAATGACTAGTAATATACTGTTTGCCGGTGGAAAAGTGGTTTTGTACCGGAGTGGCGGAAATTTGCATACTTATGACCTGAAGACTAAAGAAACTCAATGTTTGGGTGATGAGCTTGATCAGAATGCGAGGATTGTTTCTCATGTGAATAGTCTTGTGGAGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

368

Amino Acids

41.09

Weight (kDa)

8.32

Isoelectric Point (pI)

33.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 106 - 236 3.5e-06 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 108 - 232 1.1e-10 F-box protein At3g26010-like, beta-propeller
FBA_1 PF07734 110 - 240 1.5e-11 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013590)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13490
malus_domestica MD02G1150300.v1.1 MD15G1264700.v1.1 MD15G1264800.v1.1
prunus_persica Prupe.7G152800_v2.0.a1
pyrus_communis pycom02g11810 pycom15g23050 pycom15g23060
rosa_chinensis RchiOBHm_Chr2g0101881
rosa_laevigata RLG00000017107
rosa_multiflora Rmu_sc0008071.1_g000001
rosa_roxburghii Rroxscaffold_2G00140970
rosa_rugosa Rorug02G0102800
rosa_samantha Rh2AG150100 Rh2BG155700 Rh2CG156000 Rh2DG155800
rosa_wichuraiana Rw2G011750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 930
Acc36I ACCTGC 1 cut(s) 112
AccB7I CCANNNNNTGG 2 cut(s) 632, 873
AccIII TCCGGA 1 cut(s) 227
AciI CCGC 1 cut(s) 993
AclWI GGATC 1 cut(s) 448
AcoI YGGCCR 2 cut(s) 492, 607
AcsI RAATTY 1 cut(s) 997
AcuI CTGAAG 1 cut(s) 1037
AfaI GTAC 2 cut(s) 500, 983
AfiI CCNNNNNNNGG 3 cut(s) 632, 700, 873
AgsI TTSAA 2 cut(s) 224, 470
AhlI ACTAGT 1 cut(s) 944
AjnI CCWGG 4 cut(s) 351, 566, 756, 866
AluBI AGCT 6 cut(s) 151, 195, 297, 505, 778, 1051
AluI AGCT 6 cut(s) 151, 195, 297, 505, 778, 1051
Alw21I GWGCWC 1 cut(s) 712
Alw26I GTCTC 1 cut(s) 569
AlwI GGATC 1 cut(s) 448
Ama87I CYCGRG 1 cut(s) 208
Aor13HI TCCGGA 1 cut(s) 227
AoxI GGCC 3 cut(s) 492, 607, 753
ApaI GGGCCC 1 cut(s) 757
ApeKI GCWGC 1 cut(s) 348
ApoI RAATTY 1 cut(s) 997
AspS9I GGNCC 3 cut(s) 61, 753, 754
AsuHPI GGTGA 4 cut(s) 605, 826, 894, 1055
AvaI CYCGRG 1 cut(s) 208
AvaII GGWCC 1 cut(s) 61
BaeGI GKGCMC 1 cut(s) 757
BalI TGGCCA 1 cut(s) 609
BanII GRGCYC 1 cut(s) 757
BauI CACGAG 1 cut(s) 620
BbsI GAAGAC 1 cut(s) 1025
Bbv12I GWGCWC 1 cut(s) 712
BbvI GCAGC 1 cut(s) 335
BccI CCATC 8 cut(s) 295, 356, 481, 529, 618, 694, 760, 764
BciT130I CCWGG 4 cut(s) 353, 568, 758, 868
BclI TGATCA 2 cut(s) 433, 1054
BcoDI GTCTC 1 cut(s) 569
BcuI ACTAGT 1 cut(s) 944
BfaI CTAG 4 cut(s) 399, 795, 945, 1102
BfmI CTRYAG 1 cut(s) 801
BfuAI ACCTGC 1 cut(s) 112
BglII AGATCT 1 cut(s) 1098
BisI GCNGC 1 cut(s) 349
BlsI GCNGC 1 cut(s) 350
Bme1390I CCNGG 4 cut(s) 353, 568, 758, 868
Bme18I GGWCC 1 cut(s) 61
BmeT110I CYCGRG 1 cut(s) 208
BmgT120I GGNCC 3 cut(s) 61, 753, 754
BmiI GGNNCC 3 cut(s) 83, 754, 755
BmrFI CCNGG 4 cut(s) 353, 568, 758, 868
BmsI GCATC 2 cut(s) 843, 929
BpiI GAAGAC 1 cut(s) 1025
BpmI CTGGAG 4 cut(s) 77, 150, 550, 762
Bpu10I CCTNAGC 1 cut(s) 306
BsaJI CCNNGG 5 cut(s) 264, 567, 756, 757, 867
BsaWI WCCGGW 2 cut(s) 227, 984
Bsc4I CCNNNNNNNGG 3 cut(s) 632, 700, 873
Bse118I RCCGGY 2 cut(s) 310, 962
Bse1I ACTGG 5 cut(s) 94, 133, 149, 637, 745
Bse3DI GCAATG 1 cut(s) 868
BseAI TCCGGA 1 cut(s) 227
BseBI CCWGG 4 cut(s) 353, 568, 758, 868
BseDI CCNNGG 5 cut(s) 264, 567, 756, 757, 867
BseGI GGATG 3 cut(s) 577, 775, 858
BseLI CCNNNNNNNGG 3 cut(s) 632, 700, 873
BseMI GCAATG 1 cut(s) 868
BseMII CTCAG 1 cut(s) 297
BseNI ACTGG 5 cut(s) 94, 133, 149, 637, 745
BseRI GAGGAG 1 cut(s) 759
BseSI GKGCMC 1 cut(s) 757
BseXI GCAGC 1 cut(s) 335
BshFI GGCC 3 cut(s) 494, 609, 755
BsiHKAI GWGCWC 1 cut(s) 712
BsiHKCI CYCGRG 1 cut(s) 208
BsiSI CCGG 4 cut(s) 228, 311, 963, 985
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 3 cut(s) 632, 700, 873
BsmAI GTCTC 1 cut(s) 569
BsmFI GGGAC 1 cut(s) 155
BsmI GAATGC 1 cut(s) 1066
BsnI GGCC 3 cut(s) 494, 609, 755
BsoBI CYCGRG 1 cut(s) 208
Bsp120I GGGCCC 1 cut(s) 753
Bsp1286I GDGCHC 2 cut(s) 712, 757
Bsp13I TCCGGA 1 cut(s) 227
Bsp143I GATC 5 cut(s) 433, 440, 891, 1054, 1098
BspACI CCGC 1 cut(s) 993
BspANI GGCC 3 cut(s) 494, 609, 755
BspCNI CTCAG 1 cut(s) 298
BspEI TCCGGA 1 cut(s) 227
BspHI TCATGA 1 cut(s) 358
BspLI GGNNCC 3 cut(s) 83, 754, 755
BspMI ACCTGC 1 cut(s) 112
BspPI GGATC 1 cut(s) 448
BsrDI GCAATG 1 cut(s) 868
BsrFI RCCGGY 2 cut(s) 310, 962
BsrI ACTGG 5 cut(s) 94, 133, 149, 637, 745
BssAI RCCGGY 2 cut(s) 310, 962
BssECI CCNNGG 5 cut(s) 264, 567, 756, 757, 867
BssMI GATC 5 cut(s) 433, 440, 891, 1054, 1098
BssSI CACGAG 1 cut(s) 620
BssT1I CCWWGG 1 cut(s) 264
Bst2BI CACGAG 1 cut(s) 620
Bst2UI CCWGG 4 cut(s) 353, 568, 758, 868
Bst4CI ACNGT 4 cut(s) 47, 814, 912, 957
BstC8I GCNNGC 1 cut(s) 708
BstDEI CTNAG 1 cut(s) 306
BstF5I GGATG 3 cut(s) 577, 775, 858
BstKTI GATC 5 cut(s) 436, 443, 894, 1057, 1101
BstMAI GTCTC 1 cut(s) 569
BstMBI GATC 5 cut(s) 433, 440, 891, 1054, 1098
BstNI CCWGG 4 cut(s) 353, 568, 758, 868
BstSCI CCNGG 4 cut(s) 351, 566, 756, 866
BstSFI CTRYAG 1 cut(s) 801
BstSLI GKGCMC 1 cut(s) 757
BstV1I GCAGC 1 cut(s) 335
BstV2I GAAGAC 1 cut(s) 1025
BstX2I RGATCY 2 cut(s) 440, 1098
BstYI RGATCY 2 cut(s) 440, 1098
BsuRI GGCC 3 cut(s) 494, 609, 755
BtsCI GGATG 3 cut(s) 577, 775, 858
BtsIMutI CAGTG 1 cut(s) 126
BveI ACCTGC 1 cut(s) 112
Cac8I GCNNGC 1 cut(s) 708
CciI TCATGA 1 cut(s) 358
Cfr10I RCCGGY 2 cut(s) 310, 962
Cfr13I GGNCC 3 cut(s) 61, 753, 754
Csp6I GTAC 2 cut(s) 499, 982
CviAII CATG 6 cut(s) 58, 86, 359, 641, 873, 1079
CviQI GTAC 2 cut(s) 499, 982
DdeI CTNAG 1 cut(s) 306
DpnI GATC 5 cut(s) 435, 442, 893, 1056, 1100
DpnII GATC 5 cut(s) 433, 440, 891, 1054, 1098
EaeI YGGCCR 2 cut(s) 492, 607
EciI GGCGGA 1 cut(s) 1008
Eco130I CCWWGG 1 cut(s) 264
Eco24I GRGCYC 1 cut(s) 757
Eco32I GATATC 2 cut(s) 157, 205
Eco47I GGWCC 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 1037
Eco88I CYCGRG 1 cut(s) 208
EcoO109I RGGNCCY 2 cut(s) 753, 754
EcoRII CCWGG 4 cut(s) 351, 566, 756, 866
EcoRV GATATC 2 cut(s) 157, 205
EcoT14I CCWWGG 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 757
ErhI CCWWGG 1 cut(s) 264
FaeI CATG 6 cut(s) 61, 89, 362, 644, 876, 1082
FalI AAGNNNNNCTT 2 cut(s) 313, 345
FaqI GGGAC 1 cut(s) 155
FatI CATG 6 cut(s) 57, 85, 358, 640, 872, 1078
FauNDI CATATG 1 cut(s) 509
FbaI TGATCA 2 cut(s) 433, 1054
Fnu4HI GCNGC 1 cut(s) 349
FokI GGATG 3 cut(s) 584, 782, 865
FriOI GRGCYC 1 cut(s) 757
Fsp4HI GCNGC 1 cut(s) 349
FspBI CTAG 4 cut(s) 399, 795, 945, 1102
GluI GCNGC 1 cut(s) 349
GsuI CTGGAG 4 cut(s) 77, 150, 550, 762
HaeIII GGCC 3 cut(s) 494, 609, 755
HapII CCGG 4 cut(s) 228, 311, 963, 985
Hin1II CATG 6 cut(s) 61, 89, 362, 644, 876, 1082
HindIII AAGCTT 1 cut(s) 776
HinfI GANTC 2 cut(s) 4, 679
HpaII CCGG 4 cut(s) 228, 311, 963, 985
HphI GGTGA 4 cut(s) 605, 826, 894, 1055
Hpy166II GTNNAC 2 cut(s) 126, 646
Hpy188I TCNGA 6 cut(s) 678, 765, 786, 891, 925, 1059
Hpy188III TCNNGA 5 cut(s) 200, 228, 257, 281, 359
Hpy8I GTNNAC 2 cut(s) 126, 646
HpyAV CCTTC 2 cut(s) 6, 252
HpyCH4III ACNGT 4 cut(s) 47, 814, 912, 957
HpyCH4V TGCA 3 cut(s) 387, 856, 1003
HpyF3I CTNAG 1 cut(s) 306
Hsp92II CATG 6 cut(s) 61, 89, 362, 644, 876, 1082
Kpn2I TCCGGA 1 cut(s) 227
Ksp22I TGATCA 2 cut(s) 433, 1054
Kzo9I GATC 5 cut(s) 433, 440, 891, 1054, 1098
LmnI GCTCC 1 cut(s) 703
Lsp1109I GCAGC 1 cut(s) 335
LweI GCATC 2 cut(s) 843, 929
MaeI CTAG 4 cut(s) 399, 795, 945, 1102
MaeIII GTNAC 1 cut(s) 394
MalI GATC 5 cut(s) 435, 442, 893, 1056, 1100
MboI GATC 5 cut(s) 433, 440, 891, 1054, 1098
MboII GAAGA 2 cut(s) 321, 1030
MflI RGATCY 2 cut(s) 440, 1098
MhlI GDGCHC 2 cut(s) 712, 757
MlsI TGGCCA 1 cut(s) 609
MluCI AATT 3 cut(s) 484, 727, 997
MluNI TGGCCA 1 cut(s) 609
MnlI CCTC 7 cut(s) 74, 101, 574, 737, 915, 945, 1059
Mox20I TGGCCA 1 cut(s) 609
MroI TCCGGA 1 cut(s) 227
MscI TGGCCA 1 cut(s) 609
MslI CAYNNNNRTG 2 cut(s) 861, 1008
Msp20I TGGCCA 1 cut(s) 609
MspI CCGG 4 cut(s) 228, 311, 963, 985
MspR9I CCNGG 4 cut(s) 353, 568, 758, 868
Mva1269I GAATGC 1 cut(s) 1066
MvaI CCWGG 4 cut(s) 353, 568, 758, 868
NdeI CATATG 1 cut(s) 509
NdeII GATC 5 cut(s) 433, 440, 891, 1054, 1098
NlaIII CATG 6 cut(s) 61, 89, 362, 644, 876, 1082
NlaIV GGNNCC 3 cut(s) 83, 754, 755
NmeAIII GCCGAG 1 cut(s) 520
PagI TCATGA 1 cut(s) 358
PasI CCCWGGG 1 cut(s) 757
PctI GAATGC 1 cut(s) 1066
PfeI GAWTC 2 cut(s) 4, 679
PflMI CCANNNNNTGG 2 cut(s) 632, 873
PkrI GCNGC 1 cut(s) 350
PsiI TTATAA 1 cut(s) 930
Psp6I CCWGG 4 cut(s) 351, 566, 756, 866
PspGI CCWGG 4 cut(s) 351, 566, 756, 866
PspN4I GGNNCC 3 cut(s) 83, 754, 755
PspOMI GGGCCC 1 cut(s) 753
PspPI GGNCC 3 cut(s) 61, 753, 754
PsuI RGATCY 2 cut(s) 440, 1098
RsaI GTAC 2 cut(s) 500, 983
RsaNI GTAC 2 cut(s) 499, 982
RseI CAYNNNNRTG 2 cut(s) 861, 1008
SatI GCNGC 1 cut(s) 349
Sau3AI GATC 5 cut(s) 433, 440, 891, 1054, 1098
Sau96I GGNCC 3 cut(s) 61, 753, 754
ScrFI CCNGG 4 cut(s) 353, 568, 758, 868
SduI GDGCHC 2 cut(s) 712, 757
SfaNI GCATC 2 cut(s) 843, 929
SfcI CTRYAG 1 cut(s) 801
SinI GGWCC 1 cut(s) 61
SmiMI CAYNNNNRTG 2 cut(s) 861, 1008
SpeI ACTAGT 1 cut(s) 944
Sse9I AATT 3 cut(s) 484, 727, 997
SsiI CCGC 1 cut(s) 993
SspMI CTAG 4 cut(s) 399, 795, 945, 1102
StyD4I CCNGG 4 cut(s) 351, 566, 756, 866
StyI CCWWGG 1 cut(s) 264
TaaI ACNGT 4 cut(s) 47, 814, 912, 957
TaqI TCGA 2 cut(s) 153, 937
TasI AATT 3 cut(s) 484, 727, 997
TatI WGTACW 1 cut(s) 498
TfiI GAWTC 2 cut(s) 4, 679
TscAI CASTG 1 cut(s) 133
TseI GCWGC 1 cut(s) 348
TspDTI ATGAA 4 cut(s) 17, 234, 238, 629
TspGWI ACGGA 1 cut(s) 453
TspRI CASTG 1 cut(s) 133
Van91I CCANNNNNTGG 2 cut(s) 632, 873
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 1 cut(s) 997
XcmI CCANNNNNNNNNTGG 1 cut(s) 359
XspI CTAG 4 cut(s) 399, 795, 945, 1102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.