MD15G1264700.v1.1

F-Box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013590
Physical Location & Seq
Reverse (-)
22676353 .. 22678939
2587 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1264700.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGGATCAAGAAAGGAGAACAAATAAGTTACTTGCAAACAAAAACAGTATTCTATACATGGACCTCAAGGACATCATTAAGAACCATGCCCTCGAGTTTCTACCTGCTAAATCACTTCTCAGGTTCACTGGAGTTTGTAGGGACTGGAAGCTCGATATCAAAACTCCTTTCTTTGCCCACAAGCAGTCAAATAGTTTTCGTGGAATATCGGGATTCTTTTATCAATCCAATGCAGGCCCTCCTTCATTCATCTCTATCAATCCGAATGCATATGGTGTTCCAGACCCATCTCTGAAGTTCTTGCCTGAGCCAGTTGACATACGAGCTTCTTCGAATGGATTACTTTGCTGCCAGGGACGTGATGGCTATAAGGCTTACTACATTTGCAATCCTGTTACAAGGAAGTGGACAAAACTTCCCAAGCCAAACGCGGATCACGGACATGACCCTGCTATTGTTCTCATCTTTGAACCTTCTCTGCTCAATTTTGTGGCCGAGTACAAGCTCGTGTGTGCTTTTCCATCAGTTGATTTCGACAATGGATATGAATTCGAGATTTATTCCTCTAGGGAGGAGTCTTGGAGGGTTTCCGGTGAGATTTTCTTCGGCAAGAGCACTCTCTTGCCAAGATCCGGTGTTCATGTGAACGACATAGTTTATTGGCTGGCATTGAATGGTCAGATTCTTGTTTTTGACCTGAAGATGGAGCGTGCGCAACAACTTAACAGAGGATATGGGACCCTTGGTATGATGGATGGAAAGCTTTGTTCTACGTTTTCTCAACCTCCCACACTAGTTGTACGAACGTTGTGTAATGCGTACACAAACACAATGGCGATGGGCAGCAATGTCGAGCCATGGGTAATGAAACATCAGATCAAACTGCCCGCTCCGATGTCATCTGGAACCGGTTATGGTCTAAAGTCTCGTAGTGTAGTGTTTGGCAGTGGAAATGTGATCATGTTTCGGGGTGGCGGAAAAATGCACGTTTACGACATCAAGACTAAAGAAATTCAATGTGTTTGTGATGAACTTAATTCGAGTGGGAGGCTTGTTTCTCACGTGAACAGTCTTGTGGAGCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

362

Amino Acids

40.48

Weight (kDa)

8.93

Isoelectric Point (pI)

35.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
b-prop_At3g26010-like PF24750 108 - 278 1.2e-11 F-box protein At3g26010-like, beta-propeller
FBA_3 PF08268 108 - 237 2.9e-07 F-box associated beta propeller domain
FBA_1 PF07734 110 - 240 6.5e-11 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013590)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g13490
malus_domestica MD02G1150300.v1.1 MD15G1264700.v1.1 MD15G1264800.v1.1
prunus_persica Prupe.7G152800_v2.0.a1
pyrus_communis pycom02g11810 pycom15g23050 pycom15g23060
rosa_chinensis RchiOBHm_Chr2g0101881
rosa_laevigata RLG00000017107
rosa_multiflora Rmu_sc0008071.1_g000001
rosa_roxburghii Rroxscaffold_2G00140970
rosa_rugosa Rorug02G0102800
rosa_samantha Rh2AG150100 Rh2BG155700 Rh2CG156000 Rh2DG155800
rosa_wichuraiana Rw2G011750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 714
Acc36I ACCTGC 1 cut(s) 112
AccBSI CCGCTC 1 cut(s) 890
AccII CGCG 1 cut(s) 431
AciI CCGC 3 cut(s) 431, 888, 975
AclI AACGTT 1 cut(s) 806
AclWI GGATC 3 cut(s) 12, 441, 624
AcoI YGGCCR 1 cut(s) 492
AcsI RAATTY 2 cut(s) 548, 1011
AcuI CTGAAG 2 cut(s) 314, 719
AcvI CACGTG 1 cut(s) 1063
AfaI GTAC 3 cut(s) 500, 801, 821
AfiI CCNNNNNNNGG 2 cut(s) 632, 703
AgeI ACCGGT 1 cut(s) 908
AgsI TTSAA 3 cut(s) 470, 673, 1016
AhlI ACTAGT 1 cut(s) 793
AjiI CACGTC 1 cut(s) 359
AjnI CCWGG 1 cut(s) 351
AluBI AGCT 5 cut(s) 151, 326, 505, 763, 1081
AluI AGCT 5 cut(s) 151, 326, 505, 763, 1081
Alw21I GWGCWC 2 cut(s) 617, 1083
Alw26I GTCTC 1 cut(s) 930
AlwI GGATC 3 cut(s) 12, 441, 624
Ama87I CYCGRG 1 cut(s) 92
AoxI GGCC 2 cut(s) 235, 492
ApeKI GCWGC 2 cut(s) 348, 843
ApoI RAATTY 2 cut(s) 548, 1011
AsiGI ACCGGT 1 cut(s) 908
AspLEI GCGC 1 cut(s) 715
AspS9I GGNCC 3 cut(s) 61, 236, 738
AsuHPI GGTGA 1 cut(s) 605
AsuII TTCGAA 1 cut(s) 332
AvaI CYCGRG 1 cut(s) 92
AvaII GGWCC 2 cut(s) 61, 738
BanII GRGCYC 1 cut(s) 1083
BauI CACGAG 1 cut(s) 506
BbrPI CACGTG 1 cut(s) 1063
Bbv12I GWGCWC 2 cut(s) 617, 1083
BbvI GCAGC 2 cut(s) 335, 855
BccI CCATC 7 cut(s) 295, 356, 529, 697, 745, 749, 832
BcgI CGANNNNNNTGC 2 cut(s) 832, 866
BciT130I CCWGG 1 cut(s) 353
BclI TGATCA 1 cut(s) 957
BcoDI GTCTC 1 cut(s) 930
BcuI ACTAGT 1 cut(s) 793
BfaI CTAG 3 cut(s) 567, 794, 1084
BfuAI ACCTGC 1 cut(s) 112
BisI GCNGC 2 cut(s) 349, 844
BlsI GCNGC 2 cut(s) 350, 845
Bme1390I CCNGG 1 cut(s) 353
Bme18I GGWCC 2 cut(s) 61, 738
BmeT110I CYCGRG 1 cut(s) 92
BmgBI CACGTC 1 cut(s) 359
BmgT120I GGNCC 3 cut(s) 61, 236, 738
BmiI GGNNCC 3 cut(s) 739, 740, 907
BmrFI CCNGG 1 cut(s) 353
BpmI CTGGAG 1 cut(s) 150
Bpu10I CCTNAGC 1 cut(s) 306
Bpu14I TTCGAA 1 cut(s) 332
BpuEI CTTGAG 1 cut(s) 50
BsaAI YACGTR 1 cut(s) 1063
BsaJI CCNNGG 3 cut(s) 352, 742, 857
BsaWI WCCGGW 3 cut(s) 590, 632, 908
Bsc4I CCNNNNNNNGG 2 cut(s) 632, 703
Bse118I RCCGGY 1 cut(s) 908
Bse1I ACTGG 3 cut(s) 133, 149, 311
Bse3DI GCAATG 1 cut(s) 853
BseBI CCWGG 1 cut(s) 353
BseDI CCNNGG 3 cut(s) 352, 742, 857
BseGI GGATG 1 cut(s) 760
BseLI CCNNNNNNNGG 2 cut(s) 632, 703
BseMI GCAATG 1 cut(s) 853
BseMII CTCAG 2 cut(s) 133, 297
BseNI ACTGG 3 cut(s) 133, 149, 311
BseRI GAGGAG 1 cut(s) 587
BseXI GCAGC 2 cut(s) 335, 855
Bsh1236I CGCG 1 cut(s) 431
BshFI GGCC 2 cut(s) 237, 494
BshTI ACCGGT 1 cut(s) 908
BsiHKAI GWGCWC 2 cut(s) 617, 1083
BsiHKCI CYCGRG 1 cut(s) 92
BsiSI CCGG 3 cut(s) 591, 633, 909
BslFI GGGAC 3 cut(s) 155, 369, 751
BslI CCNNNNNNNGG 2 cut(s) 632, 703
BsmAI GTCTC 1 cut(s) 930
BsmFI GGGAC 3 cut(s) 155, 369, 751
BsmI GAATGC 1 cut(s) 271
BsnI GGCC 2 cut(s) 237, 494
BsoBI CYCGRG 1 cut(s) 92
Bsp119I TTCGAA 1 cut(s) 332
Bsp1286I GDGCHC 2 cut(s) 617, 1083
Bsp143I GATC 5 cut(s) 4, 433, 629, 876, 957
Bsp19I CCATGG 1 cut(s) 857
BspACI CCGC 3 cut(s) 431, 888, 975
BspANI GGCC 2 cut(s) 237, 494
BspCNI CTCAG 2 cut(s) 132, 298
BspFNI CGCG 1 cut(s) 431
BspLI GGNNCC 3 cut(s) 739, 740, 907
BspMI ACCTGC 1 cut(s) 112
BspPI GGATC 3 cut(s) 12, 441, 624
BspT104I TTCGAA 1 cut(s) 332
BsrBI CCGCTC 1 cut(s) 890
BsrDI GCAATG 1 cut(s) 853
BsrFI RCCGGY 1 cut(s) 908
BsrI ACTGG 3 cut(s) 133, 149, 311
BssAI RCCGGY 1 cut(s) 908
BssECI CCNNGG 3 cut(s) 352, 742, 857
BssMI GATC 5 cut(s) 4, 433, 629, 876, 957
BssSI CACGAG 1 cut(s) 506
BssT1I CCWWGG 2 cut(s) 742, 857
Bst2BI CACGAG 1 cut(s) 506
Bst2UI CCWGG 1 cut(s) 353
Bst4CI ACNGT 2 cut(s) 47, 1070
BstBAI YACGTR 1 cut(s) 1063
BstBI TTCGAA 1 cut(s) 332
BstC8I GCNNGC 4 cut(s) 235, 666, 711, 888
BstDEI CTNAG 2 cut(s) 119, 306
BstDSI CCRYGG 1 cut(s) 857
BstF5I GGATG 1 cut(s) 760
BstFNI CGCG 1 cut(s) 431
BstHHI GCGC 1 cut(s) 715
BstKTI GATC 5 cut(s) 7, 436, 632, 879, 960
BstMAI GTCTC 1 cut(s) 930
BstMBI GATC 5 cut(s) 4, 433, 629, 876, 957
BstNI CCWGG 1 cut(s) 353
BstSCI CCNGG 1 cut(s) 351
BstUI CGCG 1 cut(s) 431
BstV1I GCAGC 2 cut(s) 335, 855
BstX2I RGATCY 1 cut(s) 629
BstYI RGATCY 1 cut(s) 629
BsuRI GGCC 2 cut(s) 237, 494
BtgI CCRYGG 1 cut(s) 857
BtgZI GCGATG 1 cut(s) 851
BtrI CACGTC 1 cut(s) 359
BtsCI GGATG 1 cut(s) 760
BtsI GCAGTG 1 cut(s) 952
BtsIMutI CAGTG 2 cut(s) 126, 952
BveI ACCTGC 1 cut(s) 112
Cac8I GCNNGC 4 cut(s) 235, 666, 711, 888
CfoI GCGC 1 cut(s) 715
Cfr10I RCCGGY 1 cut(s) 908
Cfr13I GGNCC 3 cut(s) 61, 236, 738
Csp6I GTAC 3 cut(s) 499, 800, 820
CspAI ACCGGT 1 cut(s) 908
CspCI CAANNNNNGTGG 2 cut(s) 778, 813
CviAII CATG 6 cut(s) 58, 86, 443, 641, 858, 961
CviQI GTAC 3 cut(s) 499, 800, 820
DdeI CTNAG 2 cut(s) 119, 306
DpnI GATC 5 cut(s) 6, 435, 631, 878, 959
DpnII GATC 5 cut(s) 4, 433, 629, 876, 957
EaeI YGGCCR 1 cut(s) 492
EciI GGCGGA 1 cut(s) 990
Ecl136II GAGCTC 1 cut(s) 1081
Eco130I CCWWGG 2 cut(s) 742, 857
Eco24I GRGCYC 1 cut(s) 1083
Eco32I GATATC 1 cut(s) 157
Eco47I GGWCC 2 cut(s) 61, 738
Eco53kI GAGCTC 1 cut(s) 1081
Eco57I CTGAAG 2 cut(s) 314, 719
Eco72I CACGTG 1 cut(s) 1063
Eco88I CYCGRG 1 cut(s) 92
EcoICRI GAGCTC 1 cut(s) 1081
EcoO109I RGGNCCY 2 cut(s) 236, 738
EcoRI GAATTC 1 cut(s) 548
EcoRII CCWGG 1 cut(s) 351
EcoRV GATATC 1 cut(s) 157
EcoT14I CCWWGG 2 cut(s) 742, 857
EcoT22I ATGCAT 1 cut(s) 271
EcoT38I GRGCYC 1 cut(s) 1083
ErhI CCWWGG 2 cut(s) 742, 857
FaeI CATG 6 cut(s) 61, 89, 446, 644, 861, 964
FaqI GGGAC 3 cut(s) 155, 369, 751
FatI CATG 6 cut(s) 57, 85, 442, 640, 857, 960
FauI CCCGC 1 cut(s) 895
FauNDI CATATG 1 cut(s) 271
FbaI TGATCA 1 cut(s) 957
Fnu4HI GCNGC 2 cut(s) 349, 844
FokI GGATG 1 cut(s) 767
FriOI GRGCYC 1 cut(s) 1083
Fsp4HI GCNGC 2 cut(s) 349, 844
FspBI CTAG 3 cut(s) 567, 794, 1084
FspI TGCGCA 1 cut(s) 714
GlaI GCGC 1 cut(s) 714
GluI GCNGC 2 cut(s) 349, 844
GsuI CTGGAG 1 cut(s) 150
HaeIII GGCC 2 cut(s) 237, 494
HapII CCGG 3 cut(s) 591, 633, 909
HhaI GCGC 1 cut(s) 715
Hin1II CATG 6 cut(s) 61, 89, 446, 644, 861, 964
Hin6I GCGC 1 cut(s) 713
HinP1I GCGC 1 cut(s) 713
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HindIII AAGCTT 1 cut(s) 761
HinfI GANTC 3 cut(s) 213, 575, 682
HpaII CCGG 3 cut(s) 591, 633, 909
HphI GGTGA 1 cut(s) 605
Hpy166II GTNNAC 7 cut(s) 126, 316, 408, 646, 822, 991, 1066
Hpy188I TCNGA 5 cut(s) 264, 294, 681, 876, 894
Hpy188III TCNNGA 6 cut(s) 8, 210, 281, 553, 903, 1000
Hpy8I GTNNAC 7 cut(s) 126, 316, 408, 646, 822, 991, 1066
HpyAV CCTTC 2 cut(s) 252, 483
HpyCH4III ACNGT 2 cut(s) 47, 1070
HpyCH4IV ACGT 5 cut(s) 358, 773, 806, 987, 1062
HpyCH4V TGCA 5 cut(s) 35, 233, 269, 387, 985
HpyF3I CTNAG 2 cut(s) 119, 306
HpySE526I ACGT 5 cut(s) 358, 773, 806, 987, 1062
Hsp92II CATG 6 cut(s) 61, 89, 446, 644, 861, 964
HspAI GCGC 1 cut(s) 713
KflI GGGWCCC 1 cut(s) 738
Ksp22I TGATCA 1 cut(s) 957
Kzo9I GATC 5 cut(s) 4, 433, 629, 876, 957
LmnI GCTCC 3 cut(s) 706, 895, 1078
Lsp1109I GCAGC 2 cut(s) 335, 855
MaeI CTAG 3 cut(s) 567, 794, 1084
MaeII ACGT 5 cut(s) 358, 773, 806, 987, 1062
MaeIII GTNAC 2 cut(s) 27, 394
MalI GATC 5 cut(s) 6, 435, 631, 878, 959
MbiI CCGCTC 1 cut(s) 890
MboI GATC 5 cut(s) 4, 433, 629, 876, 957
MboII GAAGA 3 cut(s) 321, 595, 712
MflI RGATCY 1 cut(s) 629
MhlI GDGCHC 2 cut(s) 617, 1083
MluCI AATT 4 cut(s) 484, 548, 1011, 1036
MlyI GAGTC 1 cut(s) 584
MnlI CCTC 9 cut(s) 74, 101, 249, 565, 574, 576, 722, 795, 1041
Mph1103I ATGCAT 1 cut(s) 271
MseI TTAA 3 cut(s) 78, 723, 1035
MslI CAYNNNNRTG 1 cut(s) 441
MspI CCGG 3 cut(s) 591, 633, 909
MspR9I CCNGG 1 cut(s) 353
Mva1269I GAATGC 1 cut(s) 271
MvaI CCWGG 1 cut(s) 353
MvnI CGCG 1 cut(s) 431
NcoI CCATGG 1 cut(s) 857
NdeI CATATG 1 cut(s) 271
NdeII GATC 5 cut(s) 4, 433, 629, 876, 957
NlaIII CATG 6 cut(s) 61, 89, 446, 644, 861, 964
NlaIV GGNNCC 3 cut(s) 739, 740, 907
NmeAIII GCCGAG 1 cut(s) 520
NsbI TGCGCA 1 cut(s) 714
NsiI ATGCAT 1 cut(s) 271
NspV TTCGAA 1 cut(s) 332
PaeR7I CTCGAG 1 cut(s) 92
PctI GAATGC 1 cut(s) 271
PfeI GAWTC 2 cut(s) 213, 682
PinAI ACCGGT 1 cut(s) 908
PkrI GCNGC 2 cut(s) 350, 845
PleI GAGTC 1 cut(s) 583
PmaCI CACGTG 1 cut(s) 1063
PmlI CACGTG 1 cut(s) 1063
PpsI GAGTC 1 cut(s) 583
Ppu21I YACGTR 1 cut(s) 1063
PpuMI RGGWCCY 1 cut(s) 738
Psp124BI GAGCTC 1 cut(s) 1083
Psp1406I AACGTT 1 cut(s) 806
Psp5II RGGWCCY 1 cut(s) 738
Psp6I CCWGG 1 cut(s) 351
PspCI CACGTG 1 cut(s) 1063
PspGI CCWGG 1 cut(s) 351
PspN4I GGNNCC 3 cut(s) 739, 740, 907
PspPI GGNCC 3 cut(s) 61, 236, 738
PspPPI RGGWCCY 1 cut(s) 738
PspXI VCTCGAGB 1 cut(s) 92
PsuI RGATCY 1 cut(s) 629
RsaI GTAC 3 cut(s) 500, 801, 821
RsaNI GTAC 3 cut(s) 499, 800, 820
RseI CAYNNNNRTG 1 cut(s) 441
SacI GAGCTC 1 cut(s) 1083
SaqAI TTAA 3 cut(s) 78, 723, 1035
SatI GCNGC 2 cut(s) 349, 844
Sau3AI GATC 5 cut(s) 4, 433, 629, 876, 957
Sau96I GGNCC 3 cut(s) 61, 236, 738
SchI GAGTC 1 cut(s) 584
ScrFI CCNGG 1 cut(s) 353
SduI GDGCHC 2 cut(s) 617, 1083
Sfr274I CTCGAG 1 cut(s) 92
SfuI TTCGAA 1 cut(s) 332
SinI GGWCC 2 cut(s) 61, 738
SlaI CTCGAG 1 cut(s) 92
SmiMI CAYNNNNRTG 1 cut(s) 441
SmlI CTYRAG 2 cut(s) 65, 92
SmoI CTYRAG 2 cut(s) 65, 92
SpeI ACTAGT 1 cut(s) 793
Sse9I AATT 4 cut(s) 484, 548, 1011, 1036
SsiI CCGC 3 cut(s) 431, 888, 975
SspMI CTAG 3 cut(s) 567, 794, 1084
SstI GAGCTC 1 cut(s) 1083
StyD4I CCNGG 1 cut(s) 351
StyI CCWWGG 2 cut(s) 742, 857
TaaI ACNGT 2 cut(s) 47, 1070
TaiI ACGT 5 cut(s) 361, 776, 809, 990, 1065
TaqI TCGA 7 cut(s) 93, 153, 332, 534, 552, 852, 1040
TasI AATT 4 cut(s) 484, 548, 1011, 1036
TatI WGTACW 1 cut(s) 498
TfiI GAWTC 2 cut(s) 213, 682
Tru1I TTAA 3 cut(s) 78, 723, 1035
Tru9I TTAA 3 cut(s) 78, 723, 1035
TscAI CASTG 2 cut(s) 133, 952
TseI GCWGC 2 cut(s) 348, 843
TspDTI ATGAA 6 cut(s) 234, 238, 561, 629, 881, 1044
TspGWI ACGGA 1 cut(s) 453
TspRI CASTG 2 cut(s) 133, 952
VpaK11BI GGWCC 2 cut(s) 61, 738
XapI RAATTY 2 cut(s) 548, 1011
XcmI CCANNNNNNNNNTGG 1 cut(s) 359
XhoI CTCGAG 1 cut(s) 92
XspI CTAG 3 cut(s) 567, 794, 1084
Zsp2I ATGCAT 1 cut(s) 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.