MD02G1157800.v1.1

oxygen-evolving enhancer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013223
Physical Location & Seq
Reverse (-)
13189819 .. 13191728
1910 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1157800.v1.1.491

Sequence Viewer

Length: 999 bp
ATGGCAGCCTCCCTCCAAGCAGCTGCTACGCTCATGCAGCCGACCAAGGTCGGCGTGTCGGCGAGGACCAGTGTGCAACTGAGGTCCTCTCAGAGTGTCTCCAAGGCCTTTGGCTTGGAGCCATCTGCTTCTGCTAGGCTCACTTGCTCTTTGCAATCTGATCTCAAGGACTTGGCTCACAAGTGCCTTGATGCATCCAAGATTGCTGGATTTGCTCTTGCTACTTCCGCTCTCGTCGTTTCGGGAGCAGGTGCAGAGGGAGCTCCAAAGAGGTTGACCTTCGACGAAATCCAGAGCAAGACCTACTTGGAAGTGAAGGGAACAGGAACTGCTAACCAATGCCCAACCATCGACGGAGGATCCGAAGCATTTGCCTTCAAACCCGGCAAGTACAGTGCCAAGAAGTTCTGCCTAGAGCCAACATCCTTCACTGTTAAGGCTGAGAGTGTGAACAAGAACGCTCCACCAGAGTTCCAAAACACCAAGCTCATGACCCGCCTAACCTACACCCTCGATGAGATCGAAGGACCTTTCGAAGTTGCTCCAGATGGCTCCATCAAGTTCGAAGAGAAAGACGGAATTGACTACGCTGCAGTGACAGTCCAGCTCCCGGGAGGCGAGCGCGTGCCCTTCCTCTTTACCATCAAGCAGCTGGTAGCCTCTGGCAAGCCCGAAAATTTCACGGGAGAGTTTCTAGTACCATCATACCGTGGATCCTCTTTCTTGGACCCCAAGGGAAGAGGAGGATCCACCGGTTATGACAATGCGGTCGCACTGCCGGCAGGAGGCAGAGGAGACGAGGAGGAGCTGGCAAAGGAGAACACCAAGAATACAGCAGCATCGAAGGGGAAGATAACACTGAGTGTGACAAAGACCAAGCCTGAGACTGGTGAGTTGATTGGGGTGTTCGAGAGCCTTCAGCCATCCGACACTGACTTGGGAGCCAAGACTCCAAAGGATGTCAAAATCCAGGGAGTTTGGTATGCTCAGCTTGATTAG

Protein Analysis

333

Amino Acids

35.08

Weight (kDa)

6.09

Isoelectric Point (pI)

35.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MSP PF01716 99 - 331 7e-100 Manganese-stabilising protein / photosystem II polypeptide
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013223)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50820 AT5G66570
fragaria_vesca FvH4_1g14430
malus_domestica MD02G1157800.v1.1 MD15G1272500.v1.1
prunus_persica Prupe.7G145900_v2.0.a1
pyrus_communis pycom02g12470 pycom15g23610
rosa_chinensis RchiOBHm_Chr2g0103161
rosa_laevigata RLG00000017214
rosa_multiflora Rmu_sc0000388.1_g000004
rosa_roxburghii Rroxscaffold_2G00139880
rosa_rugosa Rorug02G0112200
rosa_samantha Rh2AG161000 Rh2BG167700 Rh2CG168000 Rh2DG166600
rosa_wichuraiana Rw2G012630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 239
AasI GACNNNNNNGTC 1 cut(s) 767
Acc36I ACCTGC 1 cut(s) 239
AccBSI CCGCTC 1 cut(s) 230
AccII CGCG 1 cut(s) 624
AciI CCGC 3 cut(s) 228, 496, 767
AclWI GGATC 6 cut(s) 354, 367, 708, 721, 741, 754
AcsI RAATTY 1 cut(s) 676
AcuI CTGAAG 1 cut(s) 902
AdeI CACNNNGTG 1 cut(s) 863
AfaI GTAC 2 cut(s) 392, 699
AfiI CCNNNNNNNGG 3 cut(s) 610, 785, 887
AgeI ACCGGT 1 cut(s) 752
AgsI TTSAA 1 cut(s) 379
AjnI CCWGG 1 cut(s) 969
AluBI AGCT 7 cut(s) 23, 263, 487, 607, 652, 808, 991
AluI AGCT 7 cut(s) 23, 263, 487, 607, 652, 808, 991
Alw21I GWGCWC 1 cut(s) 265
Alw26I GTCTC 3 cut(s) 103, 789, 878
AlwI GGATC 6 cut(s) 354, 367, 708, 721, 741, 754
AlwNI CAGNNNCTG 1 cut(s) 329
Ama87I CYCGRG 1 cut(s) 610
AoxI GGCC 1 cut(s) 105
ApeKI GCWGC 7 cut(s) 5, 20, 23, 37, 590, 649, 836
ApoI RAATTY 1 cut(s) 676
AsiGI ACCGGT 1 cut(s) 752
AspLEI GCGC 1 cut(s) 624
AspS9I GGNCC 4 cut(s) 66, 84, 527, 727
AsuC2I CCSGG 3 cut(s) 384, 611, 612
AsuHPI GGTGA 1 cut(s) 902
AsuII TTCGAA 2 cut(s) 534, 564
AvaI CYCGRG 1 cut(s) 610
AvaII GGWCC 4 cut(s) 66, 84, 527, 727
BaeGI GKGCMC 1 cut(s) 630
BamHI GGATCC 3 cut(s) 359, 713, 746
BanII GRGCYC 1 cut(s) 265
Bbv12I GWGCWC 1 cut(s) 265
BbvI GCAGC 7 cut(s) 10, 17, 32, 49, 577, 661, 848
BccI CCATC 7 cut(s) 130, 356, 542, 563, 650, 709, 931
BcgI CGANNNNNNTGC 2 cut(s) 353, 387
BciT130I CCWGG 1 cut(s) 971
BcnI CCSGG 3 cut(s) 384, 611, 612
BcoDI GTCTC 3 cut(s) 103, 789, 878
BfaI CTAG 3 cut(s) 135, 413, 695
BfmI CTRYAG 1 cut(s) 591
BfuAI ACCTGC 1 cut(s) 239
BisI GCNGC 7 cut(s) 6, 21, 24, 38, 591, 650, 837
BlpI GCTNAGC 1 cut(s) 987
BlsI GCNGC 7 cut(s) 7, 22, 25, 39, 592, 651, 838
Bme1390I CCNGG 4 cut(s) 384, 611, 612, 971
Bme18I GGWCC 4 cut(s) 66, 84, 527, 727
BmeT110I CYCGRG 1 cut(s) 610
BmgT120I GGNCC 4 cut(s) 66, 84, 527, 727
BmiI GGNNCC 7 cut(s) 120, 361, 553, 715, 729, 748, 943
BmrFI CCNGG 4 cut(s) 384, 611, 612, 971
BmsI GCATC 3 cut(s) 181, 203, 848
BoxI GACNNNNGTC 1 cut(s) 47
BplI GAGNNNNNCTC 2 cut(s) 73, 105
BpmI CTGGAG 1 cut(s) 528
Bpu1102I GCTNAGC 1 cut(s) 987
Bpu14I TTCGAA 2 cut(s) 534, 564
BpuEI CTTGAG 1 cut(s) 149
BpuMI CCSGG 3 cut(s) 384, 611, 612
BsaJI CCNNGG 6 cut(s) 45, 102, 610, 709, 732, 970
BsaWI WCCGGW 1 cut(s) 752
Bsc4I CCNNNNNNNGG 3 cut(s) 610, 785, 887
Bse118I RCCGGY 2 cut(s) 752, 778
Bse1I ACTGG 2 cut(s) 69, 892
BseBI CCWGG 1 cut(s) 971
BseDI CCNNGG 6 cut(s) 45, 102, 610, 709, 732, 970
BseGI GGATG 4 cut(s) 194, 422, 923, 964
BseLI CCNNNNNNNGG 3 cut(s) 610, 785, 887
BseMII CTCAG 5 cut(s) 71, 104, 432, 851, 873
BseNI ACTGG 2 cut(s) 69, 892
BseRI GAGGAG 4 cut(s) 756, 807, 815, 818
BseSI GKGCMC 1 cut(s) 630
BseXI GCAGC 7 cut(s) 10, 17, 32, 49, 577, 661, 848
BsgI GTGCAG 1 cut(s) 273
Bsh1236I CGCG 1 cut(s) 624
Bsh1285I CGRYCG 1 cut(s) 771
BshFI GGCC 1 cut(s) 107
BshTI ACCGGT 1 cut(s) 752
BsiEI CGRYCG 1 cut(s) 771
BsiHKAI GWGCWC 1 cut(s) 265
BsiHKCI CYCGRG 1 cut(s) 610
BsiSI CCGG 4 cut(s) 384, 611, 753, 779
BslI CCNNNNNNNGG 3 cut(s) 610, 785, 887
BsmAI GTCTC 3 cut(s) 103, 789, 878
BsmBI CGTCTC 1 cut(s) 789
BsnI GGCC 1 cut(s) 107
BsoBI CYCGRG 1 cut(s) 610
Bsp119I TTCGAA 2 cut(s) 534, 564
Bsp1286I GDGCHC 2 cut(s) 265, 630
Bsp143I GATC 5 cut(s) 160, 359, 519, 713, 746
Bsp1720I GCTNAGC 1 cut(s) 987
BspACI CCGC 3 cut(s) 228, 496, 767
BspANI GGCC 1 cut(s) 107
BspCNI CTCAG 5 cut(s) 72, 103, 433, 852, 874
BspFNI CGCG 1 cut(s) 624
BspHI TCATGA 1 cut(s) 489
BspLI GGNNCC 7 cut(s) 120, 361, 553, 715, 729, 748, 943
BspMAI CTGCAG 1 cut(s) 595
BspMI ACCTGC 1 cut(s) 239
BspPI GGATC 6 cut(s) 354, 367, 708, 721, 741, 754
BspT104I TTCGAA 2 cut(s) 534, 564
BsrBI CCGCTC 1 cut(s) 230
BsrFI RCCGGY 2 cut(s) 752, 778
BsrI ACTGG 2 cut(s) 69, 892
BssAI RCCGGY 2 cut(s) 752, 778
BssECI CCNNGG 6 cut(s) 45, 102, 610, 709, 732, 970
BssMI GATC 5 cut(s) 160, 359, 519, 713, 746
BssT1I CCWWGG 3 cut(s) 45, 102, 732
Bst2UI CCWGG 1 cut(s) 971
Bst4CI ACNGT 4 cut(s) 395, 433, 601, 710
Bst6I CTCTTC 2 cut(s) 561, 733
BstBI TTCGAA 2 cut(s) 534, 564
BstC8I GCNNGC 5 cut(s) 620, 626, 668, 780, 810
BstDEI CTNAG 6 cut(s) 80, 90, 441, 860, 882, 987
BstDSI CCRYGG 1 cut(s) 709
BstF5I GGATG 4 cut(s) 194, 422, 923, 964
BstFNI CGCG 1 cut(s) 624
BstHHI GCGC 1 cut(s) 624
BstKTI GATC 5 cut(s) 163, 362, 522, 716, 749
BstMAI GTCTC 3 cut(s) 103, 789, 878
BstMBI GATC 5 cut(s) 160, 359, 519, 713, 746
BstMCI CGRYCG 1 cut(s) 771
BstMWI GCNNNNNNNGC 5 cut(s) 37, 212, 227, 260, 779
BstNI CCWGG 1 cut(s) 971
BstPAI GACNNNNGTC 1 cut(s) 47
BstSCI CCNGG 4 cut(s) 382, 609, 610, 969
BstSFI CTRYAG 1 cut(s) 591
BstSLI GKGCMC 1 cut(s) 630
BstUI CGCG 1 cut(s) 624
BstV1I GCAGC 7 cut(s) 10, 17, 32, 49, 577, 661, 848
BstX2I RGATCY 3 cut(s) 359, 713, 746
BstYI RGATCY 3 cut(s) 359, 713, 746
BsuRI GGCC 1 cut(s) 107
BtgI CCRYGG 1 cut(s) 709
BtsCI GGATG 4 cut(s) 194, 422, 923, 964
BtsI GCAGTG 2 cut(s) 600, 773
BtsIMutI CAGTG 7 cut(s) 76, 400, 429, 600, 773, 857, 930
BveI ACCTGC 1 cut(s) 239
Cac8I GCNNGC 5 cut(s) 620, 626, 668, 780, 810
CaiI CAGNNNCTG 1 cut(s) 329
CciI TCATGA 1 cut(s) 489
CfoI GCGC 1 cut(s) 624
Cfr10I RCCGGY 2 cut(s) 752, 778
Cfr13I GGNCC 4 cut(s) 66, 84, 527, 727
Cfr9I CCCGGG 1 cut(s) 610
Csp6I GTAC 2 cut(s) 391, 698
CspAI ACCGGT 1 cut(s) 752
CviAII CATG 2 cut(s) 34, 490
CviQI GTAC 2 cut(s) 391, 698
DdeI CTNAG 6 cut(s) 80, 90, 441, 860, 882, 987
DpnI GATC 5 cut(s) 162, 361, 521, 715, 748
DpnII GATC 5 cut(s) 160, 359, 519, 713, 746
DraIII CACNNNGTG 1 cut(s) 863
DrdI GACNNNNNNGTC 1 cut(s) 767
DseDI GACNNNNNNGTC 1 cut(s) 767
Eam1104I CTCTTC 2 cut(s) 561, 733
EarI CTCTTC 2 cut(s) 561, 733
Ecl136II GAGCTC 1 cut(s) 263
Eco130I CCWWGG 3 cut(s) 45, 102, 732
Eco147I AGGCCT 1 cut(s) 107
Eco24I GRGCYC 1 cut(s) 265
Eco47I GGWCC 4 cut(s) 66, 84, 527, 727
Eco53kI GAGCTC 1 cut(s) 263
Eco57I CTGAAG 1 cut(s) 902
Eco88I CYCGRG 1 cut(s) 610
EcoICRI GAGCTC 1 cut(s) 263
EcoO109I RGGNCCY 2 cut(s) 84, 527
EcoRII CCWGG 1 cut(s) 969
EcoT14I CCWWGG 3 cut(s) 45, 102, 732
EcoT22I ATGCAT 1 cut(s) 196
EcoT38I GRGCYC 1 cut(s) 265
ErhI CCWWGG 3 cut(s) 45, 102, 732
Esp3I CGTCTC 1 cut(s) 789
FaeI CATG 2 cut(s) 37, 493
FaiI YATR 5 cut(s) 35, 491, 706, 759, 984
FalI AAGNNNNNCTT 2 cut(s) 290, 322
FatI CATG 2 cut(s) 33, 489
FauI CCCGC 1 cut(s) 503
Fnu4HI GCNGC 7 cut(s) 6, 21, 24, 38, 591, 650, 837
FokI GGATG 4 cut(s) 181, 409, 910, 971
FriOI GRGCYC 1 cut(s) 265
Fsp4HI GCNGC 7 cut(s) 6, 21, 24, 38, 591, 650, 837
FspBI CTAG 3 cut(s) 135, 413, 695
GlaI GCGC 1 cut(s) 623
GluI GCNGC 7 cut(s) 6, 21, 24, 38, 591, 650, 837
GsuI CTGGAG 1 cut(s) 528
HaeIII GGCC 1 cut(s) 107
HapII CCGG 4 cut(s) 384, 611, 753, 779
HhaI GCGC 1 cut(s) 624
Hin1II CATG 2 cut(s) 37, 493
Hin6I GCGC 1 cut(s) 622
HinP1I GCGC 1 cut(s) 622
HincII GTYRAC 1 cut(s) 276
HindII GTYRAC 1 cut(s) 276
HinfI GANTC 1 cut(s) 949
HpaII CCGG 4 cut(s) 384, 611, 753, 779
HphI GGTGA 1 cut(s) 902
Hpy166II GTNNAC 2 cut(s) 276, 451
Hpy188I TCNGA 4 cut(s) 93, 160, 364, 928
Hpy188III TCNNGA 5 cut(s) 243, 292, 490, 545, 910
Hpy8I GTNNAC 2 cut(s) 276, 451
Hpy99I CGWCG 3 cut(s) 239, 287, 356
HpyAV CCTTC 8 cut(s) 289, 310, 385, 436, 518, 640, 838, 926
HpyCH4III ACNGT 4 cut(s) 395, 433, 601, 710
HpyCH4V TGCA 6 cut(s) 37, 76, 154, 194, 254, 593
HpyF10VI GCNNNNNNNGC 5 cut(s) 37, 212, 227, 260, 779
HpyF3I CTNAG 6 cut(s) 80, 90, 441, 860, 882, 987
Hsp92II CATG 2 cut(s) 37, 493
HspAI GCGC 1 cut(s) 622
KroI GCCGGC 1 cut(s) 778
KroNI GCCGGC 1 cut(s) 780
Kzo9I GATC 5 cut(s) 160, 359, 519, 713, 746
Lsp1109I GCAGC 7 cut(s) 10, 17, 32, 49, 577, 661, 848
LweI GCATC 3 cut(s) 181, 203, 848
MaeI CTAG 3 cut(s) 135, 413, 695
MaeIII GTNAC 2 cut(s) 595, 865
MalI GATC 5 cut(s) 162, 361, 521, 715, 748
MbiI CCGCTC 1 cut(s) 230
MboI GATC 5 cut(s) 160, 359, 519, 713, 746
MboII GAAGA 3 cut(s) 578, 750, 862
MflI RGATCY 3 cut(s) 359, 713, 746
MhlI GDGCHC 2 cut(s) 265, 630
MluCI AATT 2 cut(s) 579, 676
MlyI GAGTC 1 cut(s) 943
MmeI TCCRAC 1 cut(s) 951
Mph1103I ATGCAT 1 cut(s) 196
MroNI GCCGGC 1 cut(s) 778
MseI TTAA 1 cut(s) 435
MspA1I CMGCKG 2 cut(s) 23, 652
MspI CCGG 4 cut(s) 384, 611, 753, 779
MspR9I CCNGG 4 cut(s) 384, 611, 612, 971
MvaI CCWGG 1 cut(s) 971
MvnI CGCG 1 cut(s) 624
MwoI GCNNNNNNNGC 5 cut(s) 37, 212, 227, 260, 779
NaeI GCCGGC 1 cut(s) 780
NciI CCSGG 3 cut(s) 384, 611, 612
NdeII GATC 5 cut(s) 160, 359, 519, 713, 746
NgoMIV GCCGGC 1 cut(s) 778
NlaIII CATG 2 cut(s) 37, 493
NlaIV GGNNCC 7 cut(s) 120, 361, 553, 715, 729, 748, 943
NmuCI GTSAC 2 cut(s) 595, 865
NsiI ATGCAT 1 cut(s) 196
NspV TTCGAA 2 cut(s) 534, 564
PagI TCATGA 1 cut(s) 489
PaqCI CACCTGC 1 cut(s) 239
PceI AGGCCT 1 cut(s) 107
PcsI WCGNNNNNNNCGW 2 cut(s) 360, 519
PdiI GCCGGC 1 cut(s) 780
PinAI ACCGGT 1 cut(s) 752
PkrI GCNGC 7 cut(s) 7, 22, 25, 39, 592, 651, 838
PleI GAGTC 1 cut(s) 943
PpsI GAGTC 1 cut(s) 943
PpuMI RGGWCCY 2 cut(s) 84, 527
PshAI GACNNNNGTC 1 cut(s) 47
Psp124BI GAGCTC 1 cut(s) 265
Psp5II RGGWCCY 2 cut(s) 84, 527
Psp6I CCWGG 1 cut(s) 969
PspGI CCWGG 1 cut(s) 969
PspN4I GGNNCC 7 cut(s) 120, 361, 553, 715, 729, 748, 943
PspPI GGNCC 4 cut(s) 66, 84, 527, 727
PspPPI RGGWCCY 2 cut(s) 84, 527
PstI CTGCAG 1 cut(s) 595
PstNI CAGNNNCTG 1 cut(s) 329
PsuI RGATCY 3 cut(s) 359, 713, 746
PvuII CAGCTG 2 cut(s) 23, 652
RsaI GTAC 2 cut(s) 392, 699
RsaNI GTAC 2 cut(s) 391, 698
SacI GAGCTC 1 cut(s) 265
SaqAI TTAA 1 cut(s) 435
SatI GCNGC 7 cut(s) 6, 21, 24, 38, 591, 650, 837
Sau3AI GATC 5 cut(s) 160, 359, 519, 713, 746
Sau96I GGNCC 4 cut(s) 66, 84, 527, 727
SchI GAGTC 1 cut(s) 943
ScrFI CCNGG 4 cut(s) 384, 611, 612, 971
SduI GDGCHC 2 cut(s) 265, 630
SfaNI GCATC 3 cut(s) 181, 203, 848
SfcI CTRYAG 1 cut(s) 591
SfuI TTCGAA 2 cut(s) 534, 564
SinI GGWCC 4 cut(s) 66, 84, 527, 727
SmaI CCCGGG 1 cut(s) 612
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 2 cut(s) 579, 676
SseBI AGGCCT 1 cut(s) 107
SsiI CCGC 3 cut(s) 228, 496, 767
SspMI CTAG 3 cut(s) 135, 413, 695
SstI GAGCTC 1 cut(s) 265
StuI AGGCCT 1 cut(s) 107
StyD4I CCNGG 4 cut(s) 382, 609, 610, 969
StyI CCWWGG 3 cut(s) 45, 102, 732
TaaI ACNGT 4 cut(s) 395, 433, 601, 710
TaqI TCGA 8 cut(s) 282, 351, 513, 522, 534, 564, 842, 909
TasI AATT 2 cut(s) 579, 676
TatI WGTACW 1 cut(s) 390
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TscAI CASTG 7 cut(s) 76, 400, 436, 600, 780, 864, 937
TseFI GTSAC 2 cut(s) 595, 865
TseI GCWGC 7 cut(s) 5, 20, 23, 37, 590, 649, 836
Tsp45I GTSAC 2 cut(s) 595, 865
TspGWI ACGGA 2 cut(s) 369, 591
TspMI CCCGGG 1 cut(s) 610
TspRI CASTG 7 cut(s) 76, 400, 436, 600, 780, 864, 937
VpaK11BI GGWCC 4 cut(s) 66, 84, 527, 727
XapI RAATTY 1 cut(s) 676
XcmI CCANNNNNNNNNTGG 1 cut(s) 649
XmaI CCCGGG 1 cut(s) 610
XspI CTAG 3 cut(s) 135, 413, 695
Zsp2I ATGCAT 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.