Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0005615

Physical Location & Seq

Genomic Coordinates Forward (+)

19950017 .. 19957606

Gene Structure 7590 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1201700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2703 bp

ATGTCTTCGCGAAACGGCGTCGCGCTGAGGGGTTCCGTGAAGCTGGATCGCCCGGCCAATTTCAGAACGTCGTCGTTCAAGTCCAGGCTCCCTCCTTCTCAATCTCCCGGATCCGCCGTGCGCCGGAGCAGCCCGGCGCTGTTTGGCACCGCAGCTTCCAAGGACGACGGAGTACCCGGAAGAGTTCGAGTAGCTGTAAGATTGCGGCCCAGAAATGCGGAGGAGATGATGGCTGATGCCGATTTTGCTGACTGTGTGGAGTTGCAGCCAGAGCTTAAAAGGTTGAAACTTAGAAAGAACAATTGGGATTTGGATACCTATGAGTTTGATGATGTGCTCACGGAGTATGCATCGCAGAAGCGTGTCTATGAAGTTGTTGCCAAGCCTGTCGTGGAGAGTGTTTTAGATGGTTACAATGGCACGGTTATGGCTTACGGTCAGACAGGTACAGGGAAAACATTTACTCTTGGACAGCTTGGAGAAGAAGATACATCTGAGCGTGGAATCATGGTTCGTTCAATGGAGGACATTTTGGCAGATATATCTCCCCAGACTGATTCTATCTCTGTATCGTACCTGCAGCTTTATATGGAGACCCTACAGGATCTGCTTGATCCGGCAAATGATAATATTCCCATTGTGGAAGATCCAAGAACTGGAGATGTTTCAGTACCTGGGGCCACTATTGTAGAGATAAAAAATCAGCAAAGTTTTCTGGAGTTGTTAAGATGTGGGGAAGCTCACCGTATAGCTGCTAACACAAAATTGAACACTGAATCATCTCGTAGTCATGCGATTTTGATGGTACAAGTTAAGAGGTCTGTCTTAGGAAGAGAAGATGATGTTGCAAGTGAAAATGGTGACCCTACTCACTTGACCAAGCCTTTCAAGCCACTAGTCCGGAAAAGCAAACTTGTGGTGGTAGATTTGGCAGGTTCCGAACGTATACAAAAATCAGGAAGTGAGGGACATATGTTAGAGGAAGCTAAGTGTATCAATCTATCGCTTAGTGCACTAGGGAAGTGCATTAATTCTCTAGCAGAGAATAGTTCTCATGTTCCATTTCGCGATTCAAAGCTTACAAGATTGCTTAAAGATTCGTTTGGAGGATCAGCGAGGACTTCATTAATTGTTACTATTGGACCTTCTCCACGTCATCGGGGAGAGACTACAAGTACCATACTATTTGGACAAAGGGCTATGAAGGTGGAGAATATGTTGAAAATTAAGGAAGAGTTTGATTACAAAAGTTTGTCAAGGAAACTTGAAGTACAAGTGGACAAGCTTATTGCAGAAAATGAAAGGCAGCATAAAAATTTTGAGGATGAAATTGAAAGAATAAACCTAGAAGCACAAAAACGTGTCTCTGATGTTGAAAGAAACTTTGCTGATGCATTAGAGAAGGAGAGGCGAAAATGCCAAATGGATTATATGGAAGCTGTTAAGGAGCTGGAGGAGAAGTTGGTGTCAAATCAGAAGCCGGACCATGAGGGCCTGATTGATATTAAATGTAACGGAGAGGGGTCCGGGCTTTCTCCTTCTAAGGAAGTGGCTGAGTTACAAGAGTTGCTTAAAAAAGAAATTGACCTCAGAAAAAAGGCTGAGGAGGAGCTGAAAAATCTCAAAAGCCTAGGGCTATATTCACAATCACAAGCTGGTGGAGATGCGGAATTTTTGAAGGTTCATAAACTTCTGGAGGAGGACGCTGAACATAAGAAGAAACTTGAAGAAGAAATAGTAATTTTACGCAGTCAGTTGTTGCAGTCGAATTTTGAAGCTGAGCAGATGAGAAGGTGTCTTGATAGAGGCGGGTCTGGAAATGGGTTTACTGGTATAGATTCTTCTGCACCTTCAGTTAGGCATTCCTATCCCAGAGACACTGGGAATGGACACAAGGCACCAGTTTCCACACTCTTTGAACAAGTTGGATTGCAAAAGATTTTGTCCTTACTTGAGTCGGATGATGCTAATGTTCGCATTCATGCTGTAAAAGTGGTAGCCAACTTAGCGGCTGAAGAGGCAAATCAGAAAAGGATAGTTGAAGCTGGTGGTCTTACTTCCTTGCTGATGCTTCTTAGAAGCTTTGAGGACGAAACCGTTCGAAGAGTGGCAGCTGGTGCCATTGCAAATCTTGCAATGAATGAAGCCAATCAAGAAATTATAATGCAGGAAGGTGGGATTAGTTTGTTAGCAACGACAGCCGCTGATGCTGATGATGCTCAAACCCTACGCATGGTTGCTGGAGCTATTGCTAATTTATGCGGCAATGATAAGCTACAAATGAAGCTAAGGTCTGAAGGTGGTATTAAGGCGTTGCTAGGAATTGTGAGGTGCGGGCATCCTGATGTTCTTTCCCAAGTGGCACGTGGTATTGCTAACTTTGCAAAATGTGAATCACGAGCGTGCACTCAAGGGATAAAGAATGGAAGATCTTTCTTGATAGAAGATGGTGCGCTTCCATGGATTGTACAAAATGCTAATGACGAGGCTGCACCAATCCGGCGCCACATTGAGCTTGCACTCTGTCACTTAGCACAACATGAGGTGAATGCAAAGGACATGATTAGTGGAGGTGCCCTCTGGGAGCTAGTTAGAATTTCCCGAGACTGTTCACGAGAGGACATAAGGAGTCTTGCTCATCGGACTTTGAATTCAAGCCCCACTTTCCGTGCTGAAATGCGACGGTTACGGATAGACTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko04144 ko04728 map04144 map04728

Pfam Domains

Protein Families

Arm Kinesin

Protein Analysis

901

Amino Acids

99.51

Weight (kDa)

6.09

Isoelectric Point (pI)

47.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	60 - 204	3.4e-16	Microtubule binding
Kinesin	PF00225	67 - 403	1.2e-96	Kinesin motor domain
ARM_ARMC5	PF24768	665 - 869	9.4e-16	ARMC5
Arm	PF00514	675 - 712	9.3e-06	Armadillo/beta-catenin-like repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005615)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01950 AT1G01950 AT1G01950 AT1G01950
fragaria_vesca	FvH4_2g41300 FvH4_7g12050 FvH4_7g12050 FvH4_7g12050 FvH4_7g12050 FvH4_7g12050 FvH4_7g12050
malus_domestica	MD02G1201700.v1.1
prunus_persica	Prupe.2G147100_v2.0.a1 Prupe.2G147100_v2.0.a1 Prupe.2G147100_v2.0.a1 Prupe.2G147100_v2.0.a1
pyrus_communis	pycom02g16510
rosa_chinensis	RchiOBHm_Chr1g0350761
rosa_laevigata	RLG00000028455
rosa_multiflora	Rmu_sc0006200.1_g000002 Rmu_sc0008591.1_g000001
rosa_roxburghii	Rroxscaffold_4G00304510
rosa_rugosa	Rorug01G0214000
rosa_samantha	Rh1AG228400 Rh1BG197800 Rh1CG213200 Rh1DG225700
rosa_wichuraiana	Rw1G019780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2162
Acc36I	ACCTGC	2 cut(s)	585, 923
AccB1I	GGYRCC	5 cut(s)	146, 1897, 2117, 2502, 2573
AccB7I	CCANNNNNTGG	1 cut(s)	656
AccI	GTMKAC	1 cut(s)	946
AccII	CGCG	3 cut(s)	10, 23, 1068
AccIII	TCCGGA	1 cut(s)	900
AclWI	GGATC	7 cut(s)	54, 105, 118, 608, 612, 641, 1117
AcoI	YGGCCR	1 cut(s)	54
AcsI	RAATTY	5 cut(s)	1315, 1670, 1768, 2595, 2650
AcuI	CTGAAG	3 cut(s)	1836, 2034, 2316
AcvI	CACGTG	1 cut(s)	2366
AcyI	GRCGYC	2 cut(s)	18, 2503
AfaI	GTAC	8 cut(s)	174, 448, 575, 672, 807, 1177, 1272, 2469
AfiI	CCNNNNNNNGG	3 cut(s)	123, 656, 2233
AflIII	ACRYGT	1 cut(s)	1360
AhlI	ACTAGT	1 cut(s)	895
AjiI	CACGTC	1 cut(s)	1154
AjnI	CCWGG	2 cut(s)	83, 673
AjuI	GAANNNNNNNTTGG	2 cut(s)	286, 318
AleI	CACNNNNGTG	1 cut(s)	2401
AloI	GAACNNNNNNTCC	2 cut(s)	495, 527
Alw21I	GWGCWC	3 cut(s)	339, 1015, 2408
Alw26I	GTCTC	5 cut(s)	587, 1160, 1369, 1869, 2598
Alw44I	GTGCAC	2 cut(s)	1011, 2404
AlwI	GGATC	7 cut(s)	54, 105, 118, 608, 612, 641, 1117
AlwNI	CAGNNNCTG	3 cut(s)	607, 674, 2204
Ama87I	CYCGRG	1 cut(s)	2601
Aor13HI	TCCGGA	1 cut(s)	900
AoxI	GGCC	4 cut(s)	54, 206, 678, 1492
ApaLI	GTGCAC	2 cut(s)	1011, 2404
ApeKI	GCWGC	8 cut(s)	129, 152, 265, 580, 752, 1306, 2111, 2489
ApoI	RAATTY	5 cut(s)	1315, 1670, 1768, 2595, 2650
AseI	ATTAAT	2 cut(s)	1029, 1127
Asp700I	GAANNNNTTC	1 cut(s)	2097
AspA2I	CCTAGG	1 cut(s)	1630
AspLEI	GCGC	5 cut(s)	25, 123, 139, 2455, 2505
AspS9I	GGNCC	6 cut(s)	207, 678, 1142, 1483, 1492, 1524
AsuC2I	CCSGG	5 cut(s)	53, 108, 134, 177, 1528
AsuHPI	GGTGA	3 cut(s)	734, 872, 2557
AsuII	TTCGAA	1 cut(s)	2101
AvaI	CYCGRG	1 cut(s)	2601
AvaII	GGWCC	3 cut(s)	1142, 1483, 1524
AvrII	CCTAGG	1 cut(s)	1630
BaeGI	GKGCMC	3 cut(s)	1015, 2408, 2578
BamHI	GGATCC	1 cut(s)	110
BanI	GGYRCC	5 cut(s)	146, 1897, 2117, 2502, 2573
BarI	GAAGNNNNNNTAC	2 cut(s)	1825, 1857
BauI	CACGAG	2 cut(s)	2397, 2613
BbrPI	CACGTG	1 cut(s)	2366
Bbv12I	GWGCWC	3 cut(s)	339, 1015, 2408
BbvCI	CCTCAGC	2 cut(s)	26, 1602
BbvI	GCAGC	8 cut(s)	141, 164, 277, 592, 739, 1318, 2123, 2476
BccI	CCATC	4 cut(s)	223, 401, 796, 2441
BceAI	ACGGC	2 cut(s)	31, 101
BciT130I	CCWGG	2 cut(s)	85, 675
BciVI	GTATCC	1 cut(s)	307
BcnI	CCSGG	5 cut(s)	53, 108, 134, 177, 1528
BcoDI	GTCTC	5 cut(s)	587, 1160, 1369, 1869, 2598
BcuI	ACTAGT	1 cut(s)	895
BfaI	CTAG	7 cut(s)	896, 1016, 1037, 1346, 1631, 2318, 2588
BfmI	CTRYAG	2 cut(s)	578, 599
BfoI	RGCGCY	2 cut(s)	140, 2506
BfuAI	ACCTGC	2 cut(s)	585, 923
BfuI	GTATCC	1 cut(s)	307
BglII	AGATCT	1 cut(s)	2429
BlnI	CCTAGG	1 cut(s)	1630
BlpI	GCTNAGC	1 cut(s)	1779
Bme1390I	CCNGG	7 cut(s)	53, 85, 108, 134, 177, 675, 1528
Bme18I	GGWCC	3 cut(s)	1142, 1483, 1524
BmeT110I	CYCGRG	1 cut(s)	2601
BmgBI	CACGTC	1 cut(s)	1154
BmgT120I	GGNCC	6 cut(s)	207, 678, 1142, 1483, 1492, 1524
BmrFI	CCNGG	7 cut(s)	53, 85, 108, 134, 177, 675, 1528
BmrI	ACTGGG	1 cut(s)	1890
BmsI	GCATC	9 cut(s)	226, 359, 1381, 1654, 1954, 2058, 2197, 2206, 2347
BmuI	ACTGGG	1 cut(s)	1890
BplI	GAGNNNNNCTC	4 cut(s)	2562, 2594, 2620, 2652
BpmI	CTGGAG	5 cut(s)	678, 737, 1472, 1715, 2262
Bpu10I	CCTNAGC	3 cut(s)	26, 1602, 2288
Bpu1102I	GCTNAGC	1 cut(s)	1779
Bpu14I	TTCGAA	1 cut(s)	2101
BpuEI	CTTGAG	2 cut(s)	1973, 2394
BpuMI	CCSGG	5 cut(s)	53, 108, 134, 177, 1528
BsaAI	YACGTR	1 cut(s)	2366
BsaHI	GRCGYC	2 cut(s)	18, 2503
BsaI	GGTCTC	1 cut(s)	587
BsaJI	CCNNGG	4 cut(s)	159, 674, 1630, 2459
BsaWI	WCCGGW	1 cut(s)	900
BsaXI	ACNNNNNCTCC	4 cut(s)	1202, 1232, 1445, 1475
Bsc4I	CCNNNNNNNGG	3 cut(s)	123, 656, 2233
Bse1I	ACTGG	4 cut(s)	661, 1834, 1885, 1901
Bse3DI	GCAATG	3 cut(s)	2121, 2142, 2272
BseAI	TCCGGA	1 cut(s)	900
BseBI	CCWGG	2 cut(s)	85, 675
BseDI	CCNNGG	4 cut(s)	159, 674, 1630, 2459
BseGI	GGATG	3 cut(s)	1330, 1966, 2338
BseLI	CCNNNNNNNGG	3 cut(s)	123, 656, 2233
BseMI	GCAATG	3 cut(s)	2121, 2142, 2272
BseMII	CTCAG	6 cut(s)	17, 486, 1545, 1593, 1603, 1770
BseNI	ACTGG	4 cut(s)	661, 1834, 1885, 1901
BseRI	GAGGAG	5 cut(s)	236, 1469, 1619, 1622, 1712
BseSI	GKGCMC	3 cut(s)	1015, 2408, 2578
BseXI	GCAGC	8 cut(s)	141, 164, 277, 592, 739, 1318, 2123, 2476
BsgI	GTGCAG	2 cut(s)	1830, 2475
Bsh1236I	CGCG	3 cut(s)	10, 23, 1068
BshFI	GGCC	4 cut(s)	56, 208, 680, 1494
BshNI	GGYRCC	5 cut(s)	146, 1897, 2117, 2502, 2573
BsiHKAI	GWGCWC	3 cut(s)	339, 1015, 2408
BsiHKCI	CYCGRG	1 cut(s)	2601
BslFI	GGGAC	1 cut(s)	981
BslI	CCNNNNNNNGG	3 cut(s)	123, 656, 2233
BsmAI	GTCTC	5 cut(s)	587, 1160, 1369, 1869, 2598
BsmFI	GGGAC	1 cut(s)	981
BsmI	GAATGC	3 cut(s)	1861, 1977, 2554
BsnI	GGCC	4 cut(s)	56, 208, 680, 1494
Bso31I	GGTCTC	1 cut(s)	587
BsoBI	CYCGRG	1 cut(s)	2601
Bsp119I	TTCGAA	1 cut(s)	2101
Bsp1286I	GDGCHC	4 cut(s)	339, 1015, 2408, 2578
Bsp13I	TCCGGA	1 cut(s)	900
Bsp1407I	TGTACA	1 cut(s)	2467
Bsp143I	GATC	7 cut(s)	46, 110, 604, 613, 646, 1109, 2429
Bsp1720I	GCTNAGC	1 cut(s)	1779
Bsp19I	CCATGG	1 cut(s)	2459
Bsp68I	TCGCGA	2 cut(s)	10, 1068
BspANI	GGCC	4 cut(s)	56, 208, 680, 1494
BspCNI	CTCAG	6 cut(s)	18, 487, 1546, 1594, 1602, 1771
BspEI	TCCGGA	1 cut(s)	900
BspFNI	CGCG	3 cut(s)	10, 23, 1068
BspMAI	CTGCAG	1 cut(s)	582
BspMI	ACCTGC	2 cut(s)	585, 923
BspPI	GGATC	7 cut(s)	54, 105, 118, 608, 612, 641, 1117
BspT104I	TTCGAA	1 cut(s)	2101
BspT107I	GGYRCC	5 cut(s)	146, 1897, 2117, 2502, 2573
BspTNI	GGTCTC	1 cut(s)	587
BsrDI	GCAATG	3 cut(s)	2121, 2142, 2272
BsrGI	TGTACA	1 cut(s)	2467
BsrI	ACTGG	4 cut(s)	661, 1834, 1885, 1901
BssECI	CCNNGG	4 cut(s)	159, 674, 1630, 2459
BssMI	GATC	7 cut(s)	46, 110, 604, 613, 646, 1109, 2429
BssNAI	GTATAC	1 cut(s)	947
BssNI	GRCGYC	2 cut(s)	18, 2503
BssSI	CACGAG	2 cut(s)	2397, 2613
BssT1I	CCWWGG	3 cut(s)	159, 1630, 2459
Bst1107I	GTATAC	1 cut(s)	947
Bst2BI	CACGAG	2 cut(s)	2397, 2613
Bst2UI	CCWGG	2 cut(s)	85, 675
Bst4CI	ACNGT	7 cut(s)	254, 424, 437, 746, 2098, 2609, 2685
Bst6I	CTCTTC	5 cut(s)	175, 826, 1227, 2010, 2098
BstACI	GRCGYC	2 cut(s)	18, 2503
BstAPI	GCANNNNNTGC	2 cut(s)	2117, 2132
BstAUI	TGTACA	1 cut(s)	2467
BstBAI	YACGTR	1 cut(s)	2366
BstBI	TTCGAA	1 cut(s)	2101
BstC8I	GCNNGC	3 cut(s)	2336, 2404, 2517
BstDSI	CCRYGG	1 cut(s)	2459
BstEII	GGTNACC	1 cut(s)	860
BstF5I	GGATG	3 cut(s)	1330, 1966, 2338
BstFNI	CGCG	3 cut(s)	10, 23, 1068
BstH2I	RGCGCY	2 cut(s)	140, 2506
BstHHI	GCGC	5 cut(s)	25, 123, 139, 2455, 2505
BstKTI	GATC	7 cut(s)	49, 113, 607, 616, 649, 1112, 2432
BstMAI	GTCTC	5 cut(s)	587, 1160, 1369, 1869, 2598
BstMBI	GATC	7 cut(s)	46, 110, 604, 613, 646, 1109, 2429
BstNI	CCWGG	2 cut(s)	85, 675
BstPI	GGTNACC	1 cut(s)	860
BstSCI	CCNGG	7 cut(s)	51, 83, 106, 132, 175, 673, 1526
BstSFI	CTRYAG	2 cut(s)	578, 599
BstSLI	GKGCMC	3 cut(s)	1015, 2408, 2578
BstUI	CGCG	3 cut(s)	10, 23, 1068
BstV1I	GCAGC	8 cut(s)	141, 164, 277, 592, 739, 1318, 2123, 2476
BstX2I	RGATCY	4 cut(s)	110, 604, 646, 2429
BstYI	RGATCY	4 cut(s)	110, 604, 646, 2429
BstZ17I	GTATAC	1 cut(s)	947
BsuI	GTATCC	1 cut(s)	307
BsuRI	GGCC	4 cut(s)	56, 208, 680, 1494
BtgI	CCRYGG	1 cut(s)	2459
BtgZI	GCGATG	1 cut(s)	336
BtrI	CACGTC	1 cut(s)	1154
BtsCI	GGATG	3 cut(s)	1330, 1966, 2338
BtsIMutI	CAGTG	2 cut(s)	771, 1878
BtuMI	TCGCGA	2 cut(s)	10, 1068
BveI	ACCTGC	2 cut(s)	585, 923
Cac8I	GCNNGC	3 cut(s)	2336, 2404, 2517
CaiI	CAGNNNCTG	3 cut(s)	607, 674, 2204
CfoI	GCGC	5 cut(s)	25, 123, 139, 2455, 2505
Cfr13I	GGNCC	6 cut(s)	207, 678, 1142, 1483, 1492, 1524
CseI	GACGC	2 cut(s)	7, 1712
Csp6I	GTAC	8 cut(s)	173, 447, 574, 671, 806, 1176, 1271, 2468
CspCI	CAANNNNNGTGG	2 cut(s)	1897, 1932
CviAII	CATG	9 cut(s)	508, 791, 1055, 1487, 1982, 2233, 2460, 2540, 2560
CviQI	GTAC	8 cut(s)	173, 447, 574, 671, 806, 1176, 1271, 2468
DinI	GGCGCC	1 cut(s)	2504
DpnI	GATC	7 cut(s)	48, 112, 606, 615, 648, 1111, 2431
DpnII	GATC	7 cut(s)	46, 110, 604, 613, 646, 1109, 2429
EaeI	YGGCCR	1 cut(s)	54
Eam1104I	CTCTTC	5 cut(s)	175, 826, 1227, 2010, 2098
EarI	CTCTTC	5 cut(s)	175, 826, 1227, 2010, 2098
EciI	GGCGGA	1 cut(s)	103
Eco130I	CCWWGG	3 cut(s)	159, 1630, 2459
Eco31I	GGTCTC	1 cut(s)	587
Eco47I	GGWCC	3 cut(s)	1142, 1483, 1524
Eco57I	CTGAAG	3 cut(s)	1836, 2034, 2316
Eco72I	CACGTG	1 cut(s)	2366
Eco88I	CYCGRG	1 cut(s)	2601
Eco91I	GGTNACC	1 cut(s)	860
EcoO109I	RGGNCCY	1 cut(s)	1492
EcoO65I	GGTNACC	1 cut(s)	860
EcoRI	GAATTC	1 cut(s)	2650
EcoRII	CCWGG	2 cut(s)	83, 673
EcoT14I	CCWWGG	3 cut(s)	159, 1630, 2459
EcoT22I	ATGCAT	2 cut(s)	352, 1396
EgeI	GGCGCC	1 cut(s)	2504
EheI	GGCGCC	1 cut(s)	2504
ErhI	CCWWGG	3 cut(s)	159, 1630, 2459
FaeI	CATG	9 cut(s)	511, 794, 1058, 1490, 1985, 2236, 2463, 2543, 2563
FalI	AAGNNNNNCTT	4 cut(s)	1554, 1586, 2647, 2679
FaqI	GGGAC	1 cut(s)	981
FatI	CATG	9 cut(s)	507, 790, 1054, 1486, 1981, 2232, 2459, 2539, 2559
FauI	CCCGC	2 cut(s)	1802, 2327
FauNDI	CATATG	1 cut(s)	972
FblI	GTMKAC	1 cut(s)	946
FokI	GGATG	3 cut(s)	1337, 1973, 2325
FspBI	CTAG	7 cut(s)	896, 1016, 1037, 1346, 1631, 2318, 2588
GlaI	GCGC	5 cut(s)	24, 122, 138, 2454, 2504
GsuI	CTGGAG	5 cut(s)	678, 737, 1472, 1715, 2262
HaeII	RGCGCY	2 cut(s)	140, 2506
HaeIII	GGCC	4 cut(s)	56, 208, 680, 1494
HgaI	GACGC	2 cut(s)	7, 1712
HhaI	GCGC	5 cut(s)	25, 123, 139, 2455, 2505
Hin1I	GRCGYC	2 cut(s)	18, 2503
Hin1II	CATG	9 cut(s)	511, 794, 1058, 1490, 1985, 2236, 2463, 2543, 2563
Hin6I	GCGC	5 cut(s)	23, 121, 137, 2453, 2503
HinP1I	GCGC	5 cut(s)	23, 121, 137, 2453, 2503
HindIII	AAGCTT	3 cut(s)	1076, 1283, 2080
HinfI	GANTC	9 cut(s)	504, 557, 776, 1070, 1097, 1838, 1955, 2393, 2629
HphI	GGTGA	3 cut(s)	734, 872, 2557
Hpy166II	GTNNAC	6 cut(s)	947, 1013, 1279, 1827, 2406, 2612
Hpy8I	GTNNAC	6 cut(s)	947, 1013, 1279, 1827, 2406, 2612
Hpy99I	CGWCG	5 cut(s)	23, 73, 76, 170, 2685
HpyCH4III	ACNGT	7 cut(s)	254, 424, 437, 746, 2098, 2609, 2685
HpyCH4IV	ACGT	5 cut(s)	68, 943, 1153, 1360, 2365
HpySE526I	ACGT	5 cut(s)	68, 943, 1153, 1360, 2365
Hsp92I	GRCGYC	2 cut(s)	18, 2503
Hsp92II	CATG	9 cut(s)	511, 794, 1058, 1490, 1985, 2236, 2463, 2543, 2563
HspAI	GCGC	5 cut(s)	23, 121, 137, 2453, 2503
KasI	GGCGCC	1 cut(s)	2502
Kpn2I	TCCGGA	1 cut(s)	900
Kzo9I	GATC	7 cut(s)	46, 110, 604, 613, 646, 1109, 2429
LmnI	GCTCC	6 cut(s)	93, 126, 1447, 1609, 2243, 2584
Lsp1109I	GCAGC	8 cut(s)	141, 164, 277, 592, 739, 1318, 2123, 2476
LweI	GCATC	9 cut(s)	226, 359, 1381, 1654, 1954, 2058, 2197, 2206, 2347
MaeI	CTAG	7 cut(s)	896, 1016, 1037, 1346, 1631, 2318, 2588
MaeII	ACGT	5 cut(s)	68, 943, 1153, 1360, 2365
MaeIII	GTNAC	7 cut(s)	410, 860, 1132, 1511, 1557, 2525, 2685
MalI	GATC	7 cut(s)	48, 112, 606, 615, 648, 1111, 2431
MboI	GATC	7 cut(s)	46, 110, 604, 613, 646, 1109, 2429
MfeI	CAATTG	1 cut(s)	301
MflI	RGATCY	4 cut(s)	110, 604, 646, 2429
MhlI	GDGCHC	4 cut(s)	339, 1015, 2408, 2578
Mly113I	GGCGCC	1 cut(s)	2503
MlyI	GAGTC	2 cut(s)	1964, 2638
MmeI	TCCRAC	2 cut(s)	1906, 1938
Mph1103I	ATGCAT	2 cut(s)	352, 1396
MroI	TCCGGA	1 cut(s)	900
MroXI	GAANNNNTTC	1 cut(s)	2097
MslI	CAYNNNNRTG	3 cut(s)	425, 2343, 2401
MspA1I	CMGCKG	2 cut(s)	2114, 2204
MspR9I	CCNGG	7 cut(s)	53, 85, 108, 134, 177, 675, 1528
MunI	CAATTG	1 cut(s)	301
Mva1269I	GAATGC	3 cut(s)	1861, 1977, 2554
MvaI	CCWGG	2 cut(s)	85, 675
MvnI	CGCG	3 cut(s)	10, 23, 1068
NarI	GGCGCC	1 cut(s)	2503
NciI	CCSGG	5 cut(s)	53, 108, 134, 177, 1528
NcoI	CCATGG	1 cut(s)	2459
NdeI	CATATG	1 cut(s)	972
NdeII	GATC	7 cut(s)	46, 110, 604, 613, 646, 1109, 2429
NlaIII	CATG	9 cut(s)	511, 794, 1058, 1490, 1985, 2236, 2463, 2543, 2563
NmuCI	GTSAC	2 cut(s)	860, 2525
NruI	TCGCGA	2 cut(s)	10, 1068
NsiI	ATGCAT	2 cut(s)	352, 1396
NspV	TTCGAA	1 cut(s)	2101
OliI	CACNNNNGTG	1 cut(s)	2401
PctI	GAATGC	3 cut(s)	1861, 1977, 2554
PdmI	GAANNNNTTC	1 cut(s)	2097
PfeI	GAWTC	7 cut(s)	504, 557, 776, 1070, 1097, 1838, 2393
PflMI	CCANNNNNTGG	1 cut(s)	656
PfoI	TCCNGGA	1 cut(s)	106
PleI	GAGTC	2 cut(s)	1963, 2637
PluTI	GGCGCC	1 cut(s)	2506
PmaCI	CACGTG	1 cut(s)	2366
PmlI	CACGTG	1 cut(s)	2366
PpsI	GAGTC	2 cut(s)	1963, 2637
Ppu21I	YACGTR	1 cut(s)	2366
PshBI	ATTAAT	2 cut(s)	1029, 1127
PsiI	TTATAA	1 cut(s)	2162
Psp6I	CCWGG	2 cut(s)	83, 673
PspCI	CACGTG	1 cut(s)	2366
PspEI	GGTNACC	1 cut(s)	860
PspGI	CCWGG	2 cut(s)	83, 673
PspPI	GGNCC	6 cut(s)	207, 678, 1142, 1483, 1492, 1524
PstI	CTGCAG	1 cut(s)	582
PstNI	CAGNNNCTG	3 cut(s)	607, 674, 2204
PsuI	RGATCY	4 cut(s)	110, 604, 646, 2429
PvuII	CAGCTG	1 cut(s)	2114
RruI	TCGCGA	2 cut(s)	10, 1068
RsaI	GTAC	8 cut(s)	174, 448, 575, 672, 807, 1177, 1272, 2469
RsaNI	GTAC	8 cut(s)	173, 447, 574, 671, 806, 1176, 1271, 2468
RseI	CAYNNNNRTG	3 cut(s)	425, 2343, 2401
Sau3AI	GATC	7 cut(s)	46, 110, 604, 613, 646, 1109, 2429
Sau96I	GGNCC	6 cut(s)	207, 678, 1142, 1483, 1492, 1524
SchI	GAGTC	2 cut(s)	1964, 2638
ScrFI	CCNGG	7 cut(s)	53, 85, 108, 134, 177, 675, 1528
SduI	GDGCHC	4 cut(s)	339, 1015, 2408, 2578
SfaNI	GCATC	9 cut(s)	226, 359, 1381, 1654, 1954, 2058, 2197, 2206, 2347
SfcI	CTRYAG	2 cut(s)	578, 599
SfoI	GGCGCC	1 cut(s)	2504
SfuI	TTCGAA	1 cut(s)	2101
SinI	GGWCC	3 cut(s)	1142, 1483, 1524
SmiMI	CAYNNNNRTG	3 cut(s)	425, 2343, 2401
SmlI	CTYRAG	2 cut(s)	1952, 2409
SmoI	CTYRAG	2 cut(s)	1952, 2409
SpeI	ACTAGT	1 cut(s)	895
SspDI	GGCGCC	1 cut(s)	2502
SspI	AATATT	1 cut(s)	631
SspMI	CTAG	7 cut(s)	896, 1016, 1037, 1346, 1631, 2318, 2588
StyD4I	CCNGG	7 cut(s)	51, 83, 106, 132, 175, 673, 1526
StyI	CCWWGG	3 cut(s)	159, 1630, 2459
TaaI	ACNGT	7 cut(s)	254, 424, 437, 746, 2098, 2609, 2685
TaiI	ACGT	5 cut(s)	71, 946, 1156, 1363, 2368
TaqI	TCGA	3 cut(s)	187, 1766, 2101
TatI	WGTACW	2 cut(s)	1270, 2467
TauI	GCSGC	4 cut(s)	208, 2012, 2204, 2265
TfiI	GAWTC	7 cut(s)	504, 557, 776, 1070, 1097, 1838, 2393
TscAI	CASTG	2 cut(s)	778, 1885
TseFI	GTSAC	2 cut(s)	860, 2525
TseI	GCWGC	8 cut(s)	129, 152, 265, 580, 752, 1306, 2111, 2489
Tsp45I	GTSAC	2 cut(s)	860, 2525
TspGWI	ACGGA	5 cut(s)	25, 183, 356, 1530, 2657
TspRI	CASTG	2 cut(s)	778, 1885
Van91I	CCANNNNNTGG	1 cut(s)	656
VneI	GTGCAC	2 cut(s)	1011, 2404
VpaK11BI	GGWCC	3 cut(s)	1142, 1483, 1524
VspI	ATTAAT	2 cut(s)	1029, 1127
XapI	RAATTY	5 cut(s)	1315, 1670, 1768, 2595, 2650
XcmI	CCANNNNNNNNNTGG	2 cut(s)	388, 2363
XmaJI	CCTAGG	1 cut(s)	1630
XmiI	GTMKAC	1 cut(s)	946
XmnI	GAANNNNTTC	1 cut(s)	2097
XspI	CTAG	7 cut(s)	896, 1016, 1037, 1346, 1631, 2318, 2588
Zsp2I	ATGCAT	2 cut(s)	352, 1396

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.