MD02G1203100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014366
Physical Location & Seq
Reverse (-)
20212616 .. 20214532
1917 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1203100.v1.1.491

Sequence Viewer

Length: 1917 bp
ATGAATCCTCACAAAACGGAAGATCTCTTCCACCATCACTTCCACCACCACCAACACCAACACCAACACCACCTCAGCCACCATGATCTGGGCCCACCTCCCCACCAGATCATGGAATCCCCGCCCTCCATTTCACTCCCTGCAGACGACGTCGTCTTCCCACTCCCCCCGGAAATCGACCTCTTCCGCTCCCCCTCCTCCTCTCCCTCCCACTCCTCCGACGAAAACGACGACGTCCCAAACCCTAACCCCAAATCCGACCCCAAATCCCAGCCCATCTACATCTCCCCGGAGCCCCACATCTCCTCCCAGTTCTACACCTTCAACCCGGATTCCCATTCCCTCATGATCAGGTGCATCCTCGACGCCCGCCTCGCCACCCCCGTCGAGATCCGCGCCGCCACGCCCCGCCCCGTCCTCAAGTCCTGGCGCGCCGTCTGGAAGGACCGCAACGAGGACACCGCCTACGTCACCGCCTGGAAGCGCATCCAGGACAAGCTCGCCGCCCACGTCGACCAAAACGGTAACGAATTCTTGTGCTTCAAGAACAATAACCAGCAATTCGTTTCTCACATTAATCAGTGGCAAGACATTGTTATGAGTTTCCATAGCGATGCCGATTTGAAACACTTAGGGTTGAAGGAAACCATTGATAGGATTAAGCAGGTTTGGACTGTAGGTGCTAAGTTTTATGGAATTCCTGAGAGTTACATTAGGGTTTGTGTTGCTGCATGCACCATTTGCTCCACGGATAATTCGGGGCTCTCAGGGAGGGGCAAACGCCGGCGGTTTGAGTATACCGAGTCTTTTGATGTGCCTGCTAAAGAGGTGCCCACTAGGCTTCAGCAGTTGGCTGCTAAGCATAAGGTGGTTCTTTGTATAAGGCAGAAGTATATCAGGTATAAGCCCTTTATGGCGGAGGTTAAGGACTATGCTTGTCATAGGGCCGGGGAGCCCGCTGCTAAGAAGTCCAAGATTTTGAAGAGGGAACCATATGCTTCGAAGAGGTGCGGCTGTGGGTTTCGGATTAGGGCCATTGTTCCAATTACAAATTACAATGAGAAGGACAAGACTTTTGTGTATCAGGAAGAGGGGGTGGCAGTGTTTAAGCTGTATGCTGTGCATTCAGGGCATGAGCCAGGTCCATTGGACGGGAATGCGAGGATTATGCATCGGGTTGTGGGGCACAAAGGTGGGATTTTTATGGATCAGGATACTGTGTATGGGGTCAGTGAGGAATTGGACAATGATGGATTTGGATTGTTCGGGAAGGATGAGGGGGACATGCAGTTGTTAGTCATGCAACAGGTGCAGGAATTGAGAGCTGAAGTTGGGCTTTTAGAAGAGAAAATTGTGAAAATGCCGCAAGAGCTTTTGGGTTCAGTGTCTCGAGATTTGTTTGATCTTGTCAATAAAGTTAGACAAGTAGGAGAGGAGAGTTCAAAGCCAATGGATTTGCTACAGGATAAGACACTTGCAGGTGATATTTTGGTTGGGGATAATGATTTGGCCCATTGGAGTGATCACCATCACGAACGTTTATATGGAGATGGCAAGGACACTGATCTGATTGAGGATGATGAAGACAGTTTTGGGCGAACTCTTGGGGATGGTGTCCCTTGGGAGCAGCTGAGGGAAGACTGTAGGAGTCCGAAAGATCTGATCGGTGACCCTTGTAAGCCTGAAAAGTGGTTGAAGTGCAGTGAATTTGATGAGAAGAGCATGCTTGATTGCGAGGATACGAAACTAACCAAGTCCTTAAGACATGATGAGGGTATAGGGGCAGATGTTGGTCTTGTTGGTATACAGGTCGATAGCTTCTATCAAGCGAATTCGAAATGGTTTGATTCTCCTTGTGGGTTGGATCCAGCTGCCGATTGTGGGGATAATACGTTCAGGAATGAGGAGATTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

639

Amino Acids

72.37

Weight (kDa)

5.72

Isoelectric Point (pI)

48.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014366)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60850
fragaria_vesca FvH4_7g11970
malus_domestica MD02G1203100.v1.1 MD07G1123000.v1.1
prunus_persica Prupe.2G146400_v2.0.a1
pyrus_communis pycom02g16600 pycom07g11140
rosa_chinensis RchiOBHm_Chr1g0350691
rosa_laevigata RLG00000028463
rosa_multiflora Rmu_sc0002150.1_g000003
rosa_roxburghii Rroxscaffold_4G00304600
rosa_rugosa Rorug01G0213100
rosa_samantha Rh1AG227400 Rh1BG196900 Rh1CG212300 Rh1DG224800
rosa_wichuraiana Rw1G019690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1469
AasI GACNNNNNNGTC 1 cut(s) 152
AatII GACGTC 2 cut(s) 153, 237
Acc36I ACCTGC 2 cut(s) 655, 1469
AccB1I GGYRCC 1 cut(s) 829
AccB7I CCANNNNNTGG 2 cut(s) 88, 112
AccBSI CCGCTC 1 cut(s) 189
AccI GTMKAC 3 cut(s) 513, 797, 1804
AccII CGCG 2 cut(s) 396, 432
AclI AACGTT 1 cut(s) 1537
AclWI GGATC 4 cut(s) 385, 1215, 1859, 1872
AcsI RAATTY 4 cut(s) 530, 696, 1706, 1831
AcuI CTGAAG 2 cut(s) 827, 1347
AcyI GRCGYC 3 cut(s) 150, 234, 366
AfiI CCNNNNNNNGG 6 cut(s) 88, 112, 454, 654, 1151, 1881
AflII CTTAAG 1 cut(s) 1759
AgsI TTSAA 7 cut(s) 325, 544, 625, 640, 982, 1443, 1696
AjiI CACGTC 1 cut(s) 511
AjnI CCWGG 4 cut(s) 425, 476, 489, 1138
AjuI GAANNNNNNNTTGG 2 cut(s) 1575, 1607
AleI CACNNNNGTG 1 cut(s) 1191
AloI GAACNNNNNNTCC 2 cut(s) 1877, 1909
AluBI AGCT 7 cut(s) 499, 1111, 1325, 1372, 1630, 1818, 1871
AluI AGCT 7 cut(s) 499, 1111, 1325, 1372, 1630, 1818, 1871
Alw26I GTCTC 1 cut(s) 1392
AlwI GGATC 4 cut(s) 385, 1215, 1859, 1872
Ama87I CYCGRG 1 cut(s) 1389
AoxI GGCC 4 cut(s) 91, 945, 1032, 1509
ApaI GGGCCC 1 cut(s) 95
ApeKI GCWGC 5 cut(s) 728, 854, 959, 1627, 1871
ApoI RAATTY 4 cut(s) 530, 696, 1706, 1831
ArsI GACNNNNNNTTYG 2 cut(s) 1058, 1090
AscI GGCGCGCC 1 cut(s) 430
AseI ATTAAT 1 cut(s) 576
AspLEI GCGC 4 cut(s) 398, 432, 434, 486
AspS9I GGNCC 7 cut(s) 91, 92, 445, 945, 1032, 1142, 1510
AsuC2I CCSGG 4 cut(s) 170, 290, 329, 949
AsuHPI GGTGA 4 cut(s) 463, 1493, 1517, 1679
AsuII TTCGAA 2 cut(s) 1001, 1835
AvaI CYCGRG 1 cut(s) 1389
AvaII GGWCC 2 cut(s) 445, 1142
BaeGI GKGCMC 3 cut(s) 95, 834, 1188
BamHI GGATCC 1 cut(s) 1864
BanI GGYRCC 1 cut(s) 829
BanII GRGCYC 4 cut(s) 95, 297, 765, 957
BbsI GAAGAC 3 cut(s) 148, 1590, 1644
BbvCI CCTCAGC 2 cut(s) 74, 1631
BbvI GCAGC 5 cut(s) 715, 841, 946, 1639, 1858
BccI CCATC 6 cut(s) 42, 284, 1244, 1536, 1544, 1604
BceAI ACGGC 1 cut(s) 419
BcgI CGANNNNNNTGC 2 cut(s) 1150, 1184
BciT130I CCWGG 4 cut(s) 427, 478, 491, 1140
BciVI GTATCC 2 cut(s) 1207, 1732
BclI TGATCA 2 cut(s) 348, 1522
BcnI CCSGG 4 cut(s) 170, 290, 329, 949
BcoDI GTCTC 1 cut(s) 1392
BfaI CTAG 1 cut(s) 837
BfmI CTRYAG 4 cut(s) 141, 675, 1460, 1642
BfrI CTTAAG 1 cut(s) 1759
BfuAI ACCTGC 2 cut(s) 655, 1469
BfuI GTATCC 2 cut(s) 1207, 1732
BglI GCCNNNNNGGC 1 cut(s) 838
BglII AGATCT 2 cut(s) 22, 1657
BisI GCNGC 9 cut(s) 399, 504, 729, 855, 960, 1012, 1364, 1628, 1872
BlpI GCTNAGC 1 cut(s) 858
BlsI GCNGC 9 cut(s) 400, 505, 730, 856, 961, 1013, 1365, 1629, 1873
Bme1390I CCNGG 8 cut(s) 170, 290, 329, 427, 478, 491, 949, 1140
Bme18I GGWCC 2 cut(s) 445, 1142
BmeT110I CYCGRG 1 cut(s) 1389
BmgBI CACGTC 1 cut(s) 511
BmgT120I GGNCC 7 cut(s) 91, 92, 445, 945, 1032, 1142, 1510
BmiI GGNNCC 6 cut(s) 93, 294, 831, 954, 990, 1866
BmrFI CCNGG 8 cut(s) 170, 290, 329, 427, 478, 491, 949, 1140
BmrI ACTGGG 1 cut(s) 304
BmsI GCATC 4 cut(s) 366, 495, 604, 1180
BmuI ACTGGG 1 cut(s) 304
BpiI GAAGAC 3 cut(s) 148, 1590, 1644
Bpu10I CCTNAGC 2 cut(s) 74, 1631
Bpu1102I GCTNAGC 1 cut(s) 858
Bpu14I TTCGAA 2 cut(s) 1001, 1835
BpuEI CTTGAG 1 cut(s) 404
BpuMI CCSGG 4 cut(s) 170, 290, 329, 949
BsaBI GATNNNNATC 1 cut(s) 1527
BsaHI GRCGYC 3 cut(s) 150, 234, 366
BsaJI CCNNGG 5 cut(s) 168, 288, 747, 948, 1619
BsaXI ACNNNNNCTCC 2 cut(s) 290, 320
Bsc4I CCNNNNNNNGG 6 cut(s) 88, 112, 454, 654, 1151, 1881
Bse118I RCCGGY 1 cut(s) 783
Bse1I ACTGG 1 cut(s) 310
Bse8I GATNNNNATC 1 cut(s) 1527
BseBI CCWGG 4 cut(s) 427, 478, 491, 1140
BseDI CCNNGG 5 cut(s) 168, 288, 747, 948, 1619
BseGI GGATG 5 cut(s) 357, 486, 1279, 1582, 1615
BseJI GATNNNNATC 1 cut(s) 1527
BseLI CCNNNNNNNGG 6 cut(s) 88, 112, 454, 654, 1151, 1881
BseMII CTCAG 4 cut(s) 88, 693, 780, 1622
BseNI ACTGG 1 cut(s) 310
BsePI GCGCGC 1 cut(s) 430
BseRI GAGGAG 5 cut(s) 187, 190, 205, 295, 1448
BseSI GKGCMC 3 cut(s) 95, 834, 1188
BseXI GCAGC 5 cut(s) 715, 841, 946, 1639, 1858
BseYI CCCAGC 1 cut(s) 270
BsgI GTGCAG 2 cut(s) 1331, 1720
Bsh1236I CGCG 2 cut(s) 396, 432
BshFI GGCC 4 cut(s) 93, 947, 1034, 1511
BshNI GGYRCC 1 cut(s) 829
BsiHKCI CYCGRG 1 cut(s) 1389
BsiSI CCGG 5 cut(s) 170, 290, 329, 784, 948
BslFI GGGAC 3 cut(s) 221, 1295, 1601
BslI CCNNNNNNNGG 6 cut(s) 88, 112, 454, 654, 1151, 1881
BsmAI GTCTC 1 cut(s) 1392
BsmFI GGGAC 3 cut(s) 221, 1295, 1601
BsmI GAATGC 2 cut(s) 1123, 1162
BsnI GGCC 4 cut(s) 93, 947, 1034, 1511
BsoBI CYCGRG 1 cut(s) 1389
Bsp119I TTCGAA 2 cut(s) 1001, 1835
Bsp120I GGGCCC 1 cut(s) 91
Bsp1286I GDGCHC 6 cut(s) 95, 297, 765, 834, 957, 1188
Bsp1720I GCTNAGC 1 cut(s) 858
BspANI GGCC 4 cut(s) 93, 947, 1034, 1511
BspCNI CTCAG 4 cut(s) 87, 694, 779, 1623
BspFNI CGCG 2 cut(s) 396, 432
BspHI TCATGA 1 cut(s) 345
BspLI GGNNCC 6 cut(s) 93, 294, 831, 954, 990, 1866
BspMAI CTGCAG 1 cut(s) 145
BspMI ACCTGC 2 cut(s) 655, 1469
BspPI GGATC 4 cut(s) 385, 1215, 1859, 1872
BspQI GCTCTTC 1 cut(s) 1712
BspT104I TTCGAA 2 cut(s) 1001, 1835
BspT107I GGYRCC 1 cut(s) 829
BspTI CTTAAG 1 cut(s) 1759
BsrBI CCGCTC 1 cut(s) 189
BsrFI RCCGGY 1 cut(s) 783
BsrI ACTGG 1 cut(s) 310
BssAI RCCGGY 1 cut(s) 783
BssECI CCNNGG 5 cut(s) 168, 288, 747, 948, 1619
BssHII GCGCGC 1 cut(s) 430
BssNAI GTATAC 2 cut(s) 798, 1805
BssNI GRCGYC 3 cut(s) 150, 234, 366
BssT1I CCWWGG 1 cut(s) 1619
Bst1107I GTATAC 2 cut(s) 798, 1805
Bst2UI CCWGG 4 cut(s) 427, 478, 491, 1140
Bst4CI ACNGT 5 cut(s) 524, 676, 1219, 1589, 1643
Bst6I CTCTTC 7 cut(s) 32, 188, 977, 998, 1083, 1338, 1712
BstACI GRCGYC 3 cut(s) 150, 234, 366
BstAFI CTTAAG 1 cut(s) 1759
BstAPI GCANNNNNTGC 2 cut(s) 741, 1309
BstBI TTCGAA 2 cut(s) 1001, 1835
BstC8I GCNNGC 8 cut(s) 370, 432, 501, 733, 785, 819, 957, 1724
BstDEI CTNAG 8 cut(s) 74, 631, 684, 702, 766, 858, 963, 1631
BstDSI CCRYGG 1 cut(s) 747
BstEII GGTNACC 1 cut(s) 1667
BstF5I GGATG 5 cut(s) 357, 486, 1279, 1582, 1615
BstFNI CGCG 2 cut(s) 396, 432
BstHHI GCGC 4 cut(s) 398, 432, 434, 486
BstMAI GTCTC 1 cut(s) 1392
BstMWI GCNNNNNNNGC 6 cut(s) 374, 741, 838, 1129, 1309, 1369
BstNI CCWGG 4 cut(s) 427, 478, 491, 1140
BstNSI RCATGY 3 cut(s) 735, 1288, 1726
BstPI GGTNACC 1 cut(s) 1667
BstSCI CCNGG 8 cut(s) 168, 288, 327, 425, 476, 489, 947, 1138
BstSFI CTRYAG 4 cut(s) 141, 675, 1460, 1642
BstSLI GKGCMC 3 cut(s) 95, 834, 1188
BstUI CGCG 2 cut(s) 396, 432
BstV1I GCAGC 5 cut(s) 715, 841, 946, 1639, 1858
BstV2I GAAGAC 3 cut(s) 148, 1590, 1644
BstX2I RGATCY 4 cut(s) 22, 390, 1657, 1864
BstYI RGATCY 4 cut(s) 22, 390, 1657, 1864
BstZ17I GTATAC 2 cut(s) 798, 1805
BsuI GTATCC 2 cut(s) 1207, 1732
BsuRI GGCC 4 cut(s) 93, 947, 1034, 1511
BtgI CCRYGG 1 cut(s) 747
BtgZI GCGATG 1 cut(s) 627
BtrI CACGTC 1 cut(s) 511
BtsCI GGATG 5 cut(s) 357, 486, 1279, 1582, 1615
BtsI GCAGTG 2 cut(s) 1107, 1708
BtsIMutI CAGTG 6 cut(s) 587, 1107, 1237, 1389, 1560, 1708
BveI ACCTGC 2 cut(s) 655, 1469
Cac8I GCNNGC 8 cut(s) 370, 432, 501, 733, 785, 819, 957, 1724
CciI TCATGA 1 cut(s) 345
CfoI GCGC 4 cut(s) 398, 432, 434, 486
Cfr10I RCCGGY 1 cut(s) 783
Cfr13I GGNCC 7 cut(s) 91, 92, 445, 945, 1032, 1142, 1510
CseI GACGC 1 cut(s) 374
CviAII CATG 9 cut(s) 83, 112, 346, 732, 1133, 1285, 1300, 1723, 1766
DdeI CTNAG 8 cut(s) 74, 631, 684, 702, 766, 858, 963, 1631
DrdI GACNNNNNNGTC 1 cut(s) 152
DseDI GACNNNNNNGTC 1 cut(s) 152
Eam1104I CTCTTC 7 cut(s) 32, 188, 977, 998, 1083, 1338, 1712
EarI CTCTTC 7 cut(s) 32, 188, 977, 998, 1083, 1338, 1712
EciI GGCGGA 1 cut(s) 932
Eco130I CCWWGG 1 cut(s) 1619
Eco24I GRGCYC 4 cut(s) 95, 297, 765, 957
Eco47I GGWCC 2 cut(s) 445, 1142
Eco57I CTGAAG 2 cut(s) 827, 1347
Eco88I CYCGRG 1 cut(s) 1389
Eco91I GGTNACC 1 cut(s) 1667
EcoO65I GGTNACC 1 cut(s) 1667
EcoRI GAATTC 3 cut(s) 530, 696, 1831
EcoRII CCWGG 4 cut(s) 425, 476, 489, 1138
EcoT14I CCWWGG 1 cut(s) 1619
EcoT22I ATGCAT 1 cut(s) 1173
EcoT38I GRGCYC 4 cut(s) 95, 297, 765, 957
ErhI CCWWGG 1 cut(s) 1619
FaeI CATG 9 cut(s) 86, 115, 349, 735, 1136, 1288, 1303, 1726, 1769
FalI AAGNNNNNCTT 2 cut(s) 1320, 1352
FaqI GGGAC 3 cut(s) 221, 1295, 1601
FatI CATG 9 cut(s) 82, 111, 345, 731, 1132, 1284, 1299, 1722, 1765
FauI CCCGC 4 cut(s) 129, 377, 416, 964
FauNDI CATATG 1 cut(s) 994
FbaI TGATCA 2 cut(s) 348, 1522
FblI GTMKAC 3 cut(s) 513, 797, 1804
Fnu4HI GCNGC 9 cut(s) 399, 504, 729, 855, 960, 1012, 1364, 1628, 1872
FokI GGATG 5 cut(s) 344, 473, 1286, 1589, 1622
FriOI GRGCYC 4 cut(s) 95, 297, 765, 957
Fsp4HI GCNGC 9 cut(s) 399, 504, 729, 855, 960, 1012, 1364, 1628, 1872
FspBI CTAG 1 cut(s) 837
GlaI GCGC 4 cut(s) 397, 431, 433, 485
GluI GCNGC 9 cut(s) 399, 504, 729, 855, 960, 1012, 1364, 1628, 1872
GsaI CCCAGC 1 cut(s) 274
HaeIII GGCC 4 cut(s) 93, 947, 1034, 1511
HapII CCGG 5 cut(s) 170, 290, 329, 784, 948
HgaI GACGC 1 cut(s) 374
HhaI GCGC 4 cut(s) 398, 432, 434, 486
Hin1I GRCGYC 3 cut(s) 150, 234, 366
Hin1II CATG 9 cut(s) 86, 115, 349, 735, 1136, 1288, 1303, 1726, 1769
Hin6I GCGC 4 cut(s) 396, 430, 432, 484
HinP1I GCGC 4 cut(s) 396, 430, 432, 484
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HinfI GANTC 6 cut(s) 4, 116, 332, 803, 1648, 1847
HpaII CCGG 5 cut(s) 170, 290, 329, 784, 948
HphI GGTGA 4 cut(s) 463, 1493, 1517, 1679
Hpy166II GTNNAC 3 cut(s) 514, 798, 1805
Hpy188I TCNGA 6 cut(s) 220, 259, 1026, 1569, 1653, 1662
Hpy8I GTNNAC 3 cut(s) 514, 798, 1805
Hpy99I CGWCG 8 cut(s) 152, 155, 224, 233, 236, 368, 389, 515
HpyAV CCTTC 5 cut(s) 331, 436, 634, 1057, 1264
HpyCH4III ACNGT 5 cut(s) 524, 676, 1219, 1589, 1643
HpyCH4IV ACGT 6 cut(s) 150, 234, 468, 510, 1537, 1892
HpyF10VI GCNNNNNNNGC 6 cut(s) 374, 741, 838, 1129, 1309, 1369
HpyF3I CTNAG 8 cut(s) 74, 631, 684, 702, 766, 858, 963, 1631
HpySE526I ACGT 6 cut(s) 150, 234, 468, 510, 1537, 1892
Hsp92I GRCGYC 3 cut(s) 150, 234, 366
Hsp92II CATG 9 cut(s) 86, 115, 349, 735, 1136, 1288, 1303, 1726, 1769
HspAI GCGC 4 cut(s) 396, 430, 432, 484
KroI GCCGGC 1 cut(s) 783
KroNI GCCGGC 1 cut(s) 785
Ksp22I TGATCA 2 cut(s) 348, 1522
LguI GCTCTTC 1 cut(s) 1712
LmnI GCTCC 5 cut(s) 194, 292, 749, 952, 1624
Lsp1109I GCAGC 5 cut(s) 715, 841, 946, 1639, 1858
LweI GCATC 4 cut(s) 366, 495, 604, 1180
MaeI CTAG 1 cut(s) 837
MaeII ACGT 6 cut(s) 150, 234, 468, 510, 1537, 1892
MaeIII GTNAC 4 cut(s) 469, 524, 707, 1667
MbiI CCGCTC 1 cut(s) 189
MflI RGATCY 4 cut(s) 22, 390, 1657, 1864
MhlI GDGCHC 6 cut(s) 95, 297, 765, 834, 957, 1188
MlyI GAGTC 2 cut(s) 812, 1657
MmeI TCCRAC 3 cut(s) 243, 282, 1842
Mph1103I ATGCAT 1 cut(s) 1173
MreI CGCCGGCG 1 cut(s) 783
MroNI GCCGGC 1 cut(s) 783
MseI TTAA 5 cut(s) 576, 660, 924, 1107, 1760
MslI CAYNNNNRTG 4 cut(s) 596, 612, 1191, 1518
MspA1I CMGCKG 3 cut(s) 959, 1630, 1871
MspCI CTTAAG 1 cut(s) 1759
MspI CCGG 5 cut(s) 170, 290, 329, 784, 948
MspR9I CCNGG 8 cut(s) 170, 290, 329, 427, 478, 491, 949, 1140
Mva1269I GAATGC 2 cut(s) 1123, 1162
MvaI CCWGG 4 cut(s) 427, 478, 491, 1140
MvnI CGCG 2 cut(s) 396, 432
MwoI GCNNNNNNNGC 6 cut(s) 374, 741, 838, 1129, 1309, 1369
NaeI GCCGGC 1 cut(s) 785
NciI CCSGG 4 cut(s) 170, 290, 329, 949
NdeI CATATG 1 cut(s) 994
NgoMIV GCCGGC 1 cut(s) 783
NlaIII CATG 9 cut(s) 86, 115, 349, 735, 1136, 1288, 1303, 1726, 1769
NlaIV GGNNCC 6 cut(s) 93, 294, 831, 954, 990, 1866
NmuCI GTSAC 2 cut(s) 469, 1667
NsiI ATGCAT 1 cut(s) 1173
NspI RCATGY 3 cut(s) 735, 1288, 1726
NspV TTCGAA 2 cut(s) 1001, 1835
OliI CACNNNNGTG 1 cut(s) 1191
PaeI GCATGC 2 cut(s) 735, 1726
PaeR7I CTCGAG 1 cut(s) 1389
PagI TCATGA 1 cut(s) 345
PalAI GGCGCGCC 1 cut(s) 430
PaqCI CACCTGC 1 cut(s) 1469
PauI GCGCGC 1 cut(s) 430
PciSI GCTCTTC 1 cut(s) 1712
PcsI WCGNNNNNNNCGW 3 cut(s) 228, 381, 507
PctI GAATGC 2 cut(s) 1123, 1162
PdiI GCCGGC 1 cut(s) 785
PfeI GAWTC 4 cut(s) 4, 116, 332, 1847
PflFI GACNNNGTC 3 cut(s) 149, 152, 233
PflMI CCANNNNNTGG 2 cut(s) 88, 112
PfoI TCCNGGA 1 cut(s) 489
PkrI GCNGC 9 cut(s) 400, 505, 730, 856, 961, 1013, 1365, 1629, 1873
PleI GAGTC 2 cut(s) 811, 1656
PpsI GAGTC 2 cut(s) 811, 1656
PshBI ATTAAT 1 cut(s) 576
Psp1406I AACGTT 1 cut(s) 1537
Psp6I CCWGG 4 cut(s) 425, 476, 489, 1138
PspEI GGTNACC 1 cut(s) 1667
PspFI CCCAGC 1 cut(s) 270
PspGI CCWGG 4 cut(s) 425, 476, 489, 1138
PspN4I GGNNCC 6 cut(s) 93, 294, 831, 954, 990, 1866
PspOMI GGGCCC 1 cut(s) 91
PspPI GGNCC 7 cut(s) 91, 92, 445, 945, 1032, 1142, 1510
PstI CTGCAG 1 cut(s) 145
PsuI RGATCY 4 cut(s) 22, 390, 1657, 1864
PsyI GACNNNGTC 3 cut(s) 149, 152, 233
PteI GCGCGC 1 cut(s) 430
PvuII CAGCTG 2 cut(s) 1630, 1871
RseI CAYNNNNRTG 4 cut(s) 596, 612, 1191, 1518
SalI GTCGAC 1 cut(s) 512
SapI GCTCTTC 1 cut(s) 1712
SaqAI TTAA 5 cut(s) 576, 660, 924, 1107, 1760
SatI GCNGC 9 cut(s) 399, 504, 729, 855, 960, 1012, 1364, 1628, 1872
Sau96I GGNCC 7 cut(s) 91, 92, 445, 945, 1032, 1142, 1510
SchI GAGTC 2 cut(s) 812, 1657
ScrFI CCNGG 8 cut(s) 170, 290, 329, 427, 478, 491, 949, 1140
SduI GDGCHC 6 cut(s) 95, 297, 765, 834, 957, 1188
SfaNI GCATC 4 cut(s) 366, 495, 604, 1180
SfcI CTRYAG 4 cut(s) 141, 675, 1460, 1642
Sfr274I CTCGAG 1 cut(s) 1389
SfuI TTCGAA 2 cut(s) 1001, 1835
SgrAI CRCCGGYG 1 cut(s) 783
SgsI GGCGCGCC 1 cut(s) 430
SinI GGWCC 2 cut(s) 445, 1142
SlaI CTCGAG 1 cut(s) 1389
SmiMI CAYNNNNRTG 4 cut(s) 596, 612, 1191, 1518
SmlI CTYRAG 3 cut(s) 419, 1389, 1759
SmoI CTYRAG 3 cut(s) 419, 1389, 1759
SphI GCATGC 2 cut(s) 735, 1726
SspMI CTAG 1 cut(s) 837
StyD4I CCNGG 8 cut(s) 168, 288, 327, 425, 476, 489, 947, 1138
StyI CCWWGG 1 cut(s) 1619
TaaI ACNGT 5 cut(s) 524, 676, 1219, 1589, 1643
TaiI ACGT 6 cut(s) 153, 237, 471, 513, 1540, 1895
TaqI TCGA 8 cut(s) 177, 363, 387, 513, 1001, 1390, 1812, 1835
TauI GCSGC 4 cut(s) 401, 506, 1014, 1366
TfiI GAWTC 4 cut(s) 4, 116, 332, 1847
Tru1I TTAA 5 cut(s) 576, 660, 924, 1107, 1760
Tru9I TTAA 5 cut(s) 576, 660, 924, 1107, 1760
TscAI CASTG 6 cut(s) 587, 1107, 1237, 1389, 1567, 1708
TseFI GTSAC 2 cut(s) 469, 1667
TseI GCWGC 5 cut(s) 728, 854, 959, 1627, 1871
Tsp45I GTSAC 2 cut(s) 469, 1667
TspDTI ATGAA 2 cut(s) 17, 1596
TspGWI ACGGA 2 cut(s) 32, 764
TspRI CASTG 6 cut(s) 587, 1107, 1237, 1389, 1567, 1708
Tth111I GACNNNGTC 3 cut(s) 149, 152, 233
Van91I CCANNNNNTGG 2 cut(s) 88, 112
Vha464I CTTAAG 1 cut(s) 1759
VpaK11BI GGWCC 2 cut(s) 445, 1142
VspI ATTAAT 1 cut(s) 576
XapI RAATTY 4 cut(s) 530, 696, 1706, 1831
XceI RCATGY 3 cut(s) 735, 1288, 1726
XhoI CTCGAG 1 cut(s) 1389
XmiI GTMKAC 3 cut(s) 513, 797, 1804
XspI CTAG 1 cut(s) 837
ZraI GACGTC 2 cut(s) 151, 235
Zsp2I ATGCAT 1 cut(s) 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.