MD07G1123000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0014366
Physical Location & Seq
Forward (+)
15832158 .. 15835736
3579 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1123000.v1.1.491

Sequence Viewer

Length: 1905 bp
ATGAATCCTCACAAAACAGAAGATCTCTTCCACCATCATTTCCACCACAGCCACCATGATCTGGGCCCACCTTCCCACCAGATCATGGAATCCCATCCCTCCATTTCACTCCCTTCAGACGACGTCGTCCTCCACGACCCCGTCTTCCCCCTCCCCCCGGAAATCGACCTCTTCCGCTCCCCCTCCCCCTCCCCCTCCCACTCCTCCGACGAGAACGACGACGTCTCGAACCCTAACCCCAAATCCGACCCCAAATCCCAGCCCATCTACATCTCCCCGGAGCCCCACATCTCCTCCCAGTTCTACACCTTCAACCCGGAGTCTCATTCCCTCATGATCAGGTGCATCCTCGACGCCCGTCTCGCCACCCCCGCCGAGATCCGCGCCGCCACGCCCCGCCCTGTCCTCAAGTCCTGGCGCGCCGTCTGGAAGGACCGCAACGAGGACACCGCCTACGTCACCGCCTGGAAGCGCATCCAGGACAAGCTCGCCGCCCACGTCGACCAAAACGGTAACGAATTCTTGTGTTTCAAGAACAATAATCAGCAATTCGTTTCTCACATTAATCAGTGGCAAGACATTGTTATGAGTTTCCATAGCGATGCCGATTTGAAGCATTTAGGGTTGAAGGAAACCATTGATAGGATTAAGCAGGTTTGGACTGTAGGTGCTAAGTTTTATGGAATTCCTGAGAGTTACATTAGGGTTTGTGTTGCTGCATGCCCCGTTTGCTCCACGGACAGTTCGGGGCTTGCCGGGAGGGGCAAACGCCGGCGGTTTGAGTATACCGAGTCTTTTGATGTGCCTGCTAAAGAGGTGCCCACTAGGCTTCAGCAATTGGCTGCTAAGCATAAGGTGGTTCTTTGTATAAGGCAGAAGTACATCAGGTATAAGCCCTTTATGGCTGAGGTTAAGGACTATGCTTGTCATAGGGCCGGGGAGCCCGTGGCGAAGAAGTCCAAGATTTTGAAGAGGGAGCCGTATGCTTCAAAGAGGTGTGGGTGCGGGTTTCGGATTAGGGCCATTGTTCCGATTACGAATTACAATGAAAGGGACAAGACTTTTGTGTATCAGGAGGAGGGGGTGGCAGTGTTTAAGCTGTATGCTGTGCATTCAGGGCATGAGCCGGGTCCTTTGGACGGAAATGCGAGGATTATGCATCGGGTTGTGGGGCACAAAGGTGGGATTTTTATGGATCAGGATACTGTGTATGGGGTCAGTGAGGAATTGGACAATGATGGGTTTGGATTGTTCGGGAAGGATGAGGGGGACATGCAGTTTTTAGTGATGCAGCAGGTGCAGGAATTGAGAGCTGAAGTTGGGCTTTTAGAAGAGAAAATTGTGAAAATACCGCAGGAGCTTTTGAGTTCAGTGTCTCGAGATTTGTTTGATCTCGTGAATAAAGTTAGACAAGTAGGAGAGGAGAGTTCAAAGCCAATGGATTTGCTACACGATAAGACGCTTGCAGGTGATATTTTGGTTGTGGATAATGATTTGGCCCATTGGAGTGATCACCATCATGAACGTTTATATGGAGATGGCAAGGACGCTGATTTGATTGAGGATGATGAAGACAGTTTTGGGCGAACTCTTGAGGATGGTGTCCCTTGGGAGCAGCTGAGGGAAGACTGCAGGACTCCGAAAGATCTGATCGGTGACCCTTGTAAGCCCGAAAAGTGGTTGAAGTGCAGTGAATTTGATGAGAAGAGCATGCTTGATTGCGAGGATACTAAACTAACCAAGTCTTTAAGACATGATGAGGGTATAGGGGCAGATGTAGGTCTTGTTGGTATACAGGTCGATAGCTTCTATCAAGCGAATTCGAAATGGTTTGATTCTCCTTGTGGGTTGGACCCAGCTGCAGATTGTGGGGATAATACATTCAGGAATGGGGAGATTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

635

Amino Acids

71.86

Weight (kDa)

5.62

Isoelectric Point (pI)

45.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014366)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G60850
fragaria_vesca FvH4_7g11970
malus_domestica MD02G1203100.v1.1 MD07G1123000.v1.1
prunus_persica Prupe.2G146400_v2.0.a1
pyrus_communis pycom02g16600 pycom07g11140
rosa_chinensis RchiOBHm_Chr1g0350691
rosa_laevigata RLG00000028463
rosa_multiflora Rmu_sc0002150.1_g000003
rosa_roxburghii Rroxscaffold_4G00304600
rosa_rugosa Rorug01G0213100
rosa_samantha Rh1AG227400 Rh1BG196900 Rh1CG212300 Rh1DG224800
rosa_wichuraiana Rw1G019690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1285, 1457
AasI GACNNNNNNGTC 1 cut(s) 125
AatII GACGTC 2 cut(s) 126, 225
Acc36I ACCTGC 3 cut(s) 643, 1285, 1457
AccB1I GGYRCC 1 cut(s) 817
AccB7I CCANNNNNTGG 2 cut(s) 61, 85
AccBSI CCGCTC 1 cut(s) 177
AccI GTMKAC 3 cut(s) 501, 785, 1792
AccII CGCG 2 cut(s) 384, 420
AclI AACGTT 1 cut(s) 1525
AclWI GGATC 2 cut(s) 373, 1203
AcsI RAATTY 4 cut(s) 518, 684, 1694, 1819
AcuI CTGAAG 3 cut(s) 99, 815, 1335
AcyI GRCGYC 3 cut(s) 123, 222, 354
AfaI GTAC 1 cut(s) 881
AfiI CCNNNNNNNGG 7 cut(s) 61, 85, 157, 442, 642, 1139, 1677
AgsI TTSAA 8 cut(s) 313, 532, 613, 628, 970, 990, 1431, 1684
AjiI CACGTC 1 cut(s) 499
AjnI CCWGG 3 cut(s) 413, 464, 477
AjuI GAANNNNNNNTTGG 2 cut(s) 1563, 1595
AleI CACNNNNGTG 1 cut(s) 1179
AluBI AGCT 7 cut(s) 487, 1099, 1313, 1360, 1618, 1806, 1859
AluI AGCT 7 cut(s) 487, 1099, 1313, 1360, 1618, 1806, 1859
Alw26I GTCTC 4 cut(s) 229, 327, 365, 1380
AlwI GGATC 2 cut(s) 373, 1203
Ama87I CYCGRG 1 cut(s) 1377
AoxI GGCC 4 cut(s) 64, 933, 1020, 1497
ApaI GGGCCC 1 cut(s) 68
ApeKI GCWGC 5 cut(s) 716, 842, 1291, 1615, 1859
ApoI RAATTY 4 cut(s) 518, 684, 1694, 1819
ArsI GACNNNNNNTTYG 2 cut(s) 1046, 1078
AscI GGCGCGCC 1 cut(s) 418
AseI ATTAAT 1 cut(s) 564
AspLEI GCGC 4 cut(s) 386, 420, 422, 474
AspS9I GGNCC 8 cut(s) 64, 65, 433, 933, 1020, 1130, 1498, 1852
AsuC2I CCSGG 6 cut(s) 158, 278, 317, 757, 937, 1128
AsuHPI GGTGA 4 cut(s) 451, 1481, 1505, 1667
AsuII TTCGAA 1 cut(s) 1823
AvaI CYCGRG 1 cut(s) 1377
AvaII GGWCC 3 cut(s) 433, 1130, 1852
BaeGI GKGCMC 3 cut(s) 68, 822, 1176
BanI GGYRCC 1 cut(s) 817
BanII GRGCYC 3 cut(s) 68, 285, 945
BauI CACGAG 1 cut(s) 1394
BbsI GAAGAC 3 cut(s) 136, 1578, 1632
BbvCI CCTCAGC 2 cut(s) 906, 1619
BbvI GCAGC 5 cut(s) 703, 829, 1303, 1627, 1846
BccI CCATC 7 cut(s) 42, 102, 272, 1232, 1524, 1532, 1592
BceAI ACGGC 2 cut(s) 407, 964
BcgI CGANNNNNNTGC 2 cut(s) 1138, 1172
BciT130I CCWGG 3 cut(s) 415, 466, 479
BciVI GTATCC 2 cut(s) 1195, 1720
BclI TGATCA 2 cut(s) 336, 1510
BcnI CCSGG 6 cut(s) 158, 278, 317, 757, 937, 1128
BcoDI GTCTC 4 cut(s) 229, 327, 365, 1380
BfaI CTAG 1 cut(s) 825
BfmI CTRYAG 3 cut(s) 663, 1630, 1860
BfuAI ACCTGC 3 cut(s) 643, 1285, 1457
BfuI GTATCC 2 cut(s) 1195, 1720
BglI GCCNNNNNGGC 1 cut(s) 826
BglII AGATCT 2 cut(s) 22, 1645
BisI GCNGC 7 cut(s) 387, 492, 717, 843, 1292, 1616, 1860
BlpI GCTNAGC 1 cut(s) 846
BlsI GCNGC 7 cut(s) 388, 493, 718, 844, 1293, 1617, 1861
Bme1390I CCNGG 9 cut(s) 158, 278, 317, 415, 466, 479, 757, 937, 1128
Bme18I GGWCC 3 cut(s) 433, 1130, 1852
BmeT110I CYCGRG 1 cut(s) 1377
BmgBI CACGTC 1 cut(s) 499
BmgT120I GGNCC 8 cut(s) 64, 65, 433, 933, 1020, 1130, 1498, 1852
BmiI GGNNCC 7 cut(s) 66, 282, 819, 942, 978, 1131, 1854
BmrFI CCNGG 9 cut(s) 158, 278, 317, 415, 466, 479, 757, 937, 1128
BmrI ACTGGG 1 cut(s) 292
BmsI GCATC 5 cut(s) 354, 483, 592, 1168, 1278
BmuI ACTGGG 1 cut(s) 292
BoxI GACNNNNGTC 1 cut(s) 357
BpiI GAAGAC 3 cut(s) 136, 1578, 1632
Bpu10I CCTNAGC 2 cut(s) 906, 1619
Bpu1102I GCTNAGC 1 cut(s) 846
Bpu14I TTCGAA 1 cut(s) 1823
BpuEI CTTGAG 2 cut(s) 392, 1613
BpuMI CCSGG 6 cut(s) 158, 278, 317, 757, 937, 1128
BsaBI GATNNNNATC 1 cut(s) 1515
BsaHI GRCGYC 3 cut(s) 123, 222, 354
BsaJI CCNNGG 6 cut(s) 156, 276, 735, 936, 945, 1607
BsaXI ACNNNNNCTCC 2 cut(s) 278, 308
Bsc4I CCNNNNNNNGG 7 cut(s) 61, 85, 157, 442, 642, 1139, 1677
Bse118I RCCGGY 1 cut(s) 771
Bse1I ACTGG 1 cut(s) 298
Bse8I GATNNNNATC 1 cut(s) 1515
BseBI CCWGG 3 cut(s) 415, 466, 479
BseDI CCNNGG 6 cut(s) 156, 276, 735, 936, 945, 1607
BseGI GGATG 6 cut(s) 94, 345, 474, 1267, 1570, 1603
BseJI GATNNNNATC 1 cut(s) 1515
BseLI CCNNNNNNNGG 7 cut(s) 61, 85, 157, 442, 642, 1139, 1677
BseMII CTCAG 3 cut(s) 681, 897, 1610
BseNI ACTGG 1 cut(s) 298
BsePI GCGCGC 1 cut(s) 418
BseRI GAGGAG 4 cut(s) 193, 283, 1091, 1436
BseSI GKGCMC 3 cut(s) 68, 822, 1176
BseXI GCAGC 5 cut(s) 703, 829, 1303, 1627, 1846
BseYI CCCAGC 2 cut(s) 258, 1855
BsgI GTGCAG 2 cut(s) 1319, 1708
Bsh1236I CGCG 2 cut(s) 384, 420
BshFI GGCC 4 cut(s) 66, 935, 1022, 1499
BshNI GGYRCC 1 cut(s) 817
BsiHKCI CYCGRG 1 cut(s) 1377
BsiSI CCGG 7 cut(s) 158, 278, 317, 756, 772, 936, 1127
BslFI GGGAC 3 cut(s) 1067, 1283, 1589
BslI CCNNNNNNNGG 7 cut(s) 61, 85, 157, 442, 642, 1139, 1677
BsmAI GTCTC 4 cut(s) 229, 327, 365, 1380
BsmBI CGTCTC 2 cut(s) 229, 365
BsmFI GGGAC 3 cut(s) 1067, 1283, 1589
BsmI GAATGC 1 cut(s) 1111
BsnI GGCC 4 cut(s) 66, 935, 1022, 1499
BsoBI CYCGRG 1 cut(s) 1377
Bsp119I TTCGAA 1 cut(s) 1823
Bsp120I GGGCCC 1 cut(s) 64
Bsp1286I GDGCHC 5 cut(s) 68, 285, 822, 945, 1176
Bsp1720I GCTNAGC 1 cut(s) 846
BspANI GGCC 4 cut(s) 66, 935, 1022, 1499
BspCNI CTCAG 3 cut(s) 682, 898, 1611
BspFNI CGCG 2 cut(s) 384, 420
BspHI TCATGA 2 cut(s) 333, 1519
BspLI GGNNCC 7 cut(s) 66, 282, 819, 942, 978, 1131, 1854
BspMAI CTGCAG 2 cut(s) 1634, 1864
BspMI ACCTGC 3 cut(s) 643, 1285, 1457
BspPI GGATC 2 cut(s) 373, 1203
BspQI GCTCTTC 1 cut(s) 1700
BspT104I TTCGAA 1 cut(s) 1823
BspT107I GGYRCC 1 cut(s) 817
BsrBI CCGCTC 1 cut(s) 177
BsrFI RCCGGY 1 cut(s) 771
BsrI ACTGG 1 cut(s) 298
BssAI RCCGGY 1 cut(s) 771
BssECI CCNNGG 6 cut(s) 156, 276, 735, 936, 945, 1607
BssHII GCGCGC 1 cut(s) 418
BssNAI GTATAC 2 cut(s) 786, 1793
BssNI GRCGYC 3 cut(s) 123, 222, 354
BssSI CACGAG 1 cut(s) 1394
BssT1I CCWWGG 1 cut(s) 1607
Bst1107I GTATAC 2 cut(s) 786, 1793
Bst2BI CACGAG 1 cut(s) 1394
Bst2UI CCWGG 3 cut(s) 415, 466, 479
Bst4CI ACNGT 5 cut(s) 512, 664, 743, 1207, 1577
Bst6I CTCTTC 5 cut(s) 32, 176, 965, 1326, 1700
BstACI GRCGYC 3 cut(s) 123, 222, 354
BstAPI GCANNNNNTGC 1 cut(s) 1297
BstBI TTCGAA 1 cut(s) 1823
BstC8I GCNNGC 8 cut(s) 420, 489, 721, 753, 773, 807, 1464, 1712
BstDEI CTNAG 5 cut(s) 672, 690, 846, 906, 1619
BstDSI CCRYGG 2 cut(s) 735, 945
BstEII GGTNACC 1 cut(s) 1655
BstF5I GGATG 6 cut(s) 94, 345, 474, 1267, 1570, 1603
BstFNI CGCG 2 cut(s) 384, 420
BstHHI GCGC 4 cut(s) 386, 420, 422, 474
BstMAI GTCTC 4 cut(s) 229, 327, 365, 1380
BstMWI GCNNNNNNNGC 6 cut(s) 362, 371, 729, 826, 1117, 1297
BstNI CCWGG 3 cut(s) 415, 466, 479
BstNSI RCATGY 3 cut(s) 723, 1276, 1714
BstPAI GACNNNNGTC 1 cut(s) 357
BstPI GGTNACC 1 cut(s) 1655
BstSCI CCNGG 9 cut(s) 156, 276, 315, 413, 464, 477, 755, 935, 1126
BstSFI CTRYAG 3 cut(s) 663, 1630, 1860
BstSLI GKGCMC 3 cut(s) 68, 822, 1176
BstUI CGCG 2 cut(s) 384, 420
BstV1I GCAGC 5 cut(s) 703, 829, 1303, 1627, 1846
BstV2I GAAGAC 3 cut(s) 136, 1578, 1632
BstX2I RGATCY 3 cut(s) 22, 378, 1645
BstYI RGATCY 3 cut(s) 22, 378, 1645
BstZ17I GTATAC 2 cut(s) 786, 1793
BsuI GTATCC 2 cut(s) 1195, 1720
BsuRI GGCC 4 cut(s) 66, 935, 1022, 1499
BtgI CCRYGG 2 cut(s) 735, 945
BtgZI GCGATG 1 cut(s) 615
BtrI CACGTC 1 cut(s) 499
BtsCI GGATG 6 cut(s) 94, 345, 474, 1267, 1570, 1603
BtsI GCAGTG 2 cut(s) 1095, 1696
BtsIMutI CAGTG 5 cut(s) 575, 1095, 1225, 1377, 1696
BveI ACCTGC 3 cut(s) 643, 1285, 1457
Cac8I GCNNGC 8 cut(s) 420, 489, 721, 753, 773, 807, 1464, 1712
CciI TCATGA 2 cut(s) 333, 1519
CfoI GCGC 4 cut(s) 386, 420, 422, 474
Cfr10I RCCGGY 1 cut(s) 771
Cfr13I GGNCC 8 cut(s) 64, 65, 433, 933, 1020, 1130, 1498, 1852
CseI GACGC 3 cut(s) 362, 1468, 1556
Csp6I GTAC 1 cut(s) 880
CviAII CATG 9 cut(s) 56, 85, 334, 720, 1121, 1273, 1520, 1711, 1754
CviQI GTAC 1 cut(s) 880
DdeI CTNAG 5 cut(s) 672, 690, 846, 906, 1619
DrdI GACNNNNNNGTC 1 cut(s) 125
DseDI GACNNNNNNGTC 1 cut(s) 125
Eam1104I CTCTTC 5 cut(s) 32, 176, 965, 1326, 1700
EarI CTCTTC 5 cut(s) 32, 176, 965, 1326, 1700
Eco130I CCWWGG 1 cut(s) 1607
Eco24I GRGCYC 3 cut(s) 68, 285, 945
Eco47I GGWCC 3 cut(s) 433, 1130, 1852
Eco57I CTGAAG 3 cut(s) 99, 815, 1335
Eco88I CYCGRG 1 cut(s) 1377
Eco91I GGTNACC 1 cut(s) 1655
EcoO109I RGGNCCY 1 cut(s) 1130
EcoO65I GGTNACC 1 cut(s) 1655
EcoRI GAATTC 3 cut(s) 518, 684, 1819
EcoRII CCWGG 3 cut(s) 413, 464, 477
EcoT14I CCWWGG 1 cut(s) 1607
EcoT22I ATGCAT 1 cut(s) 1161
EcoT38I GRGCYC 3 cut(s) 68, 285, 945
ErhI CCWWGG 1 cut(s) 1607
Esp3I CGTCTC 2 cut(s) 229, 365
FaeI CATG 9 cut(s) 59, 88, 337, 723, 1124, 1276, 1523, 1714, 1757
FalI AAGNNNNNCTT 2 cut(s) 1308, 1340
FaqI GGGAC 3 cut(s) 1067, 1283, 1589
FatI CATG 9 cut(s) 55, 84, 333, 719, 1120, 1272, 1519, 1710, 1753
FauI CCCGC 3 cut(s) 379, 404, 998
FbaI TGATCA 2 cut(s) 336, 1510
FblI GTMKAC 3 cut(s) 501, 785, 1792
Fnu4HI GCNGC 7 cut(s) 387, 492, 717, 843, 1292, 1616, 1860
FokI GGATG 6 cut(s) 81, 332, 461, 1274, 1577, 1610
FriOI GRGCYC 3 cut(s) 68, 285, 945
Fsp4HI GCNGC 7 cut(s) 387, 492, 717, 843, 1292, 1616, 1860
FspBI CTAG 1 cut(s) 825
GlaI GCGC 4 cut(s) 385, 419, 421, 473
GluI GCNGC 7 cut(s) 387, 492, 717, 843, 1292, 1616, 1860
GsaI CCCAGC 2 cut(s) 262, 1859
HaeIII GGCC 4 cut(s) 66, 935, 1022, 1499
HapII CCGG 7 cut(s) 158, 278, 317, 756, 772, 936, 1127
HgaI GACGC 3 cut(s) 362, 1468, 1556
HhaI GCGC 4 cut(s) 386, 420, 422, 474
Hin1I GRCGYC 3 cut(s) 123, 222, 354
Hin1II CATG 9 cut(s) 59, 88, 337, 723, 1124, 1276, 1523, 1714, 1757
Hin6I GCGC 4 cut(s) 384, 418, 420, 472
HinP1I GCGC 4 cut(s) 384, 418, 420, 472
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HinfI GANTC 6 cut(s) 4, 89, 320, 791, 1636, 1835
HpaII CCGG 7 cut(s) 158, 278, 317, 756, 772, 936, 1127
HphI GGTGA 4 cut(s) 451, 1481, 1505, 1667
Hpy166II GTNNAC 3 cut(s) 502, 786, 1793
Hpy188I TCNGA 7 cut(s) 118, 208, 247, 1014, 1032, 1641, 1650
Hpy8I GTNNAC 3 cut(s) 502, 786, 1793
Hpy99I CGWCG 7 cut(s) 125, 128, 212, 221, 224, 356, 503
HpyAV CCTTC 6 cut(s) 81, 123, 319, 424, 622, 1252
HpyCH4III ACNGT 5 cut(s) 512, 664, 743, 1207, 1577
HpyCH4IV ACGT 5 cut(s) 123, 222, 456, 498, 1525
HpyF10VI GCNNNNNNNGC 6 cut(s) 362, 371, 729, 826, 1117, 1297
HpyF3I CTNAG 5 cut(s) 672, 690, 846, 906, 1619
HpySE526I ACGT 5 cut(s) 123, 222, 456, 498, 1525
Hsp92I GRCGYC 3 cut(s) 123, 222, 354
Hsp92II CATG 9 cut(s) 59, 88, 337, 723, 1124, 1276, 1523, 1714, 1757
HspAI GCGC 4 cut(s) 384, 418, 420, 472
KroI GCCGGC 1 cut(s) 771
KroNI GCCGGC 1 cut(s) 773
Ksp22I TGATCA 2 cut(s) 336, 1510
LguI GCTCTTC 1 cut(s) 1700
LmnI GCTCC 7 cut(s) 182, 280, 737, 940, 976, 1357, 1612
Lsp1109I GCAGC 5 cut(s) 703, 829, 1303, 1627, 1846
LweI GCATC 5 cut(s) 354, 483, 592, 1168, 1278
MaeI CTAG 1 cut(s) 825
MaeII ACGT 5 cut(s) 123, 222, 456, 498, 1525
MaeIII GTNAC 4 cut(s) 457, 512, 695, 1655
MbiI CCGCTC 1 cut(s) 177
MfeI CAATTG 1 cut(s) 836
MflI RGATCY 3 cut(s) 22, 378, 1645
MhlI GDGCHC 5 cut(s) 68, 285, 822, 945, 1176
MlyI GAGTC 3 cut(s) 329, 800, 1630
MmeI TCCRAC 3 cut(s) 231, 270, 1830
Mph1103I ATGCAT 1 cut(s) 1161
MreI CGCCGGCG 1 cut(s) 771
MroNI GCCGGC 1 cut(s) 771
MseI TTAA 5 cut(s) 564, 648, 912, 1095, 1748
MslI CAYNNNNRTG 5 cut(s) 584, 600, 1179, 1506, 1518
MspA1I CMGCKG 2 cut(s) 1618, 1859
MspI CCGG 7 cut(s) 158, 278, 317, 756, 772, 936, 1127
MspR9I CCNGG 9 cut(s) 158, 278, 317, 415, 466, 479, 757, 937, 1128
MunI CAATTG 1 cut(s) 836
Mva1269I GAATGC 1 cut(s) 1111
MvaI CCWGG 3 cut(s) 415, 466, 479
MvnI CGCG 2 cut(s) 384, 420
MwoI GCNNNNNNNGC 6 cut(s) 362, 371, 729, 826, 1117, 1297
NaeI GCCGGC 1 cut(s) 773
NciI CCSGG 6 cut(s) 158, 278, 317, 757, 937, 1128
NgoMIV GCCGGC 1 cut(s) 771
NlaIII CATG 9 cut(s) 59, 88, 337, 723, 1124, 1276, 1523, 1714, 1757
NlaIV GGNNCC 7 cut(s) 66, 282, 819, 942, 978, 1131, 1854
NmeAIII GCCGAG 1 cut(s) 400
NmuCI GTSAC 2 cut(s) 457, 1655
NsiI ATGCAT 1 cut(s) 1161
NspI RCATGY 3 cut(s) 723, 1276, 1714
NspV TTCGAA 1 cut(s) 1823
OliI CACNNNNGTG 1 cut(s) 1179
PaeI GCATGC 2 cut(s) 723, 1714
PaeR7I CTCGAG 1 cut(s) 1377
PagI TCATGA 2 cut(s) 333, 1519
PalAI GGCGCGCC 1 cut(s) 418
PaqCI CACCTGC 2 cut(s) 1285, 1457
PauI GCGCGC 1 cut(s) 418
PciSI GCTCTTC 1 cut(s) 1700
PcsI WCGNNNNNNNCGW 3 cut(s) 132, 216, 495
PctI GAATGC 1 cut(s) 1111
PdiI GCCGGC 1 cut(s) 773
PfeI GAWTC 3 cut(s) 4, 89, 1835
PflFI GACNNNGTC 4 cut(s) 122, 125, 140, 221
PflMI CCANNNNNTGG 2 cut(s) 61, 85
PfoI TCCNGGA 1 cut(s) 477
PkrI GCNGC 7 cut(s) 388, 493, 718, 844, 1293, 1617, 1861
PleI GAGTC 3 cut(s) 328, 799, 1630
PpsI GAGTC 3 cut(s) 328, 799, 1630
PpuMI RGGWCCY 1 cut(s) 1130
PshAI GACNNNNGTC 1 cut(s) 357
PshBI ATTAAT 1 cut(s) 564
Psp1406I AACGTT 1 cut(s) 1525
Psp5II RGGWCCY 1 cut(s) 1130
Psp6I CCWGG 3 cut(s) 413, 464, 477
PspEI GGTNACC 1 cut(s) 1655
PspFI CCCAGC 2 cut(s) 258, 1855
PspGI CCWGG 3 cut(s) 413, 464, 477
PspN4I GGNNCC 7 cut(s) 66, 282, 819, 942, 978, 1131, 1854
PspOMI GGGCCC 1 cut(s) 64
PspPI GGNCC 8 cut(s) 64, 65, 433, 933, 1020, 1130, 1498, 1852
PspPPI RGGWCCY 1 cut(s) 1130
PstI CTGCAG 2 cut(s) 1634, 1864
PsuI RGATCY 3 cut(s) 22, 378, 1645
PsyI GACNNNGTC 4 cut(s) 122, 125, 140, 221
PteI GCGCGC 1 cut(s) 418
PvuII CAGCTG 2 cut(s) 1618, 1859
RsaI GTAC 1 cut(s) 881
RsaNI GTAC 1 cut(s) 880
RseI CAYNNNNRTG 5 cut(s) 584, 600, 1179, 1506, 1518
SalI GTCGAC 1 cut(s) 500
SapI GCTCTTC 1 cut(s) 1700
SaqAI TTAA 5 cut(s) 564, 648, 912, 1095, 1748
SatI GCNGC 7 cut(s) 387, 492, 717, 843, 1292, 1616, 1860
Sau96I GGNCC 8 cut(s) 64, 65, 433, 933, 1020, 1130, 1498, 1852
SchI GAGTC 3 cut(s) 329, 800, 1630
ScrFI CCNGG 9 cut(s) 158, 278, 317, 415, 466, 479, 757, 937, 1128
SduI GDGCHC 5 cut(s) 68, 285, 822, 945, 1176
SfaNI GCATC 5 cut(s) 354, 483, 592, 1168, 1278
SfcI CTRYAG 3 cut(s) 663, 1630, 1860
Sfr274I CTCGAG 1 cut(s) 1377
SfuI TTCGAA 1 cut(s) 1823
SgrAI CRCCGGYG 1 cut(s) 771
SgsI GGCGCGCC 1 cut(s) 418
SinI GGWCC 3 cut(s) 433, 1130, 1852
SlaI CTCGAG 1 cut(s) 1377
SmiMI CAYNNNNRTG 5 cut(s) 584, 600, 1179, 1506, 1518
SmlI CTYRAG 3 cut(s) 407, 1377, 1592
SmoI CTYRAG 3 cut(s) 407, 1377, 1592
SphI GCATGC 2 cut(s) 723, 1714
SspMI CTAG 1 cut(s) 825
StyD4I CCNGG 9 cut(s) 156, 276, 315, 413, 464, 477, 755, 935, 1126
StyI CCWWGG 1 cut(s) 1607
TaaI ACNGT 5 cut(s) 512, 664, 743, 1207, 1577
TaiI ACGT 5 cut(s) 126, 225, 459, 501, 1528
TaqI TCGA 7 cut(s) 165, 227, 351, 501, 1378, 1800, 1823
TatI WGTACW 1 cut(s) 879
TauI GCSGC 2 cut(s) 389, 494
TfiI GAWTC 3 cut(s) 4, 89, 1835
Tru1I TTAA 5 cut(s) 564, 648, 912, 1095, 1748
Tru9I TTAA 5 cut(s) 564, 648, 912, 1095, 1748
TscAI CASTG 5 cut(s) 575, 1095, 1225, 1377, 1696
TseFI GTSAC 2 cut(s) 457, 1655
TseI GCWGC 5 cut(s) 716, 842, 1291, 1615, 1859
Tsp45I GTSAC 2 cut(s) 457, 1655
TspDTI ATGAA 4 cut(s) 17, 1062, 1536, 1584
TspGWI ACGGA 2 cut(s) 752, 1155
TspRI CASTG 5 cut(s) 575, 1095, 1225, 1377, 1696
Tth111I GACNNNGTC 4 cut(s) 122, 125, 140, 221
Van91I CCANNNNNTGG 2 cut(s) 61, 85
VpaK11BI GGWCC 3 cut(s) 433, 1130, 1852
VspI ATTAAT 1 cut(s) 564
XapI RAATTY 4 cut(s) 518, 684, 1694, 1819
XceI RCATGY 3 cut(s) 723, 1276, 1714
XhoI CTCGAG 1 cut(s) 1377
XmiI GTMKAC 3 cut(s) 501, 785, 1792
XspI CTAG 1 cut(s) 825
ZraI GACGTC 2 cut(s) 124, 223
Zsp2I ATGCAT 1 cut(s) 1161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.