Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0009245

Physical Location & Seq

Genomic Coordinates Forward (+)

20922209 .. 20926210

Gene Structure 4002 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1207200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2244 bp

ATGACTTCATCTTCATCTGTTACTCTGTCCCAGGCCCTCCTGGCCCGAGCCATTTCCCACCACGGCTCCAACTCCACCTCCGACCGGGTCTCCCTCTCCACAACCCTCACACTCCCCACTTTCTCCGGCCTCAAATCGGCTTCTTCTTCGTCATCGCCGTCGTCTTCCCGCCTCCCGCGCCGCAGGCTTGGGGCCAACCGGCCGGTCCGGGCTGCCGCGGTCGAGACTCTGGACAAGACCACGGAGACGTCGTTGGTGGAGAAGTCGGTGAATACGATCCGGTTCTTGGCGATTGACGCTGTGGAGAAGGCCAATTCGGGTCACCCGGGTTTGCCCATGGGATGTGCTCCAATGGGTCATATTTTGTACGACGAAATCATGAGGTACAACCCCAAGAACCCCTACTGGTTCAACCGTGACCGGTTCGTGTTGTCTGCTGGACACGGATGTATGTTGCAGTATGCTCTGCTGCACCTTGCCGGATACGACAGTGTAAAGGAAGAAGATTTGAAGAGTTTCCGTCAATGGGGAAGCAAGACCCCTGGACACCCTGAGAACTTTGAGACACCCGGTGTTGAAGTCACAACCGGTCCCTTGGGGCAAGGTATTGCGAATGCTGTTGGTTTGGCTCTTGCTGAGAAGCACTTGGCTGCACGTTACAACAAGCCAGACAGTGAGATCGTTGACCACTACACATACTGTATATTGGGTGATGGCTGTCAAATGGAGGGTATTTCAAATGAAGCTGCTTCTCTTGCAGGGCACTGGGGGCTTGGGAAGCTGATAGCCTTCTATGATGACAACCACATTTCCATTGATGGAGACACTGAGATTGCATTCACCGAAAGTGTTGACACCCGTTTTGAGGGTCTTGGGTGGCATGTTATCTGGGTGAAGAATGGAAACACTGGGTATGATGAGATTCGAGCTGCCATTAAGGAAGCAAAGGCTGTCACGGACAAACCCACTTTGATAAAGGTGACAACCACCATTGGTTTTGGATCCCCAAACAAAGCAAACTCATACAGTGTTCATGGTGCTGCACTTGGTTCCAAGGAAGTTGATGCTACCAGGAAGAACCTTGGATGGCCATATGAGCCTTTCCACGTGCCAGAGGATGTTAAAAGTCACTGGAGCCGCCATACCGCTGAAGGTTCTGCACTTGAAGTTGAATGGAATGCCAAGTTTGCAGAATATGAGAAGAAGTACGCAGAGGAAGCTGCAGAGCTGAAGTCCATCGTTAAGGGTGAGCTACCTGCTGGTTGGGAAAAAGCACTCCCGAGGTACACTCCTGAGTCCCCAGCTGACGCTACCCGAAATTTATCTCAAGCGAACCTTAATGCCCTTTCTAAGGTTCTTCCCGGTCTTATTGGAGGAAGTGCAGATCTTGCTTCTTCTAACATGACTTTACTGAAAAGTTTTGGAGACTTCCAGAAGAATACCCCAGAAGAGCGCAATGTAAGGTTTGGTGTAAGAGAGCATGGAATGGGAGCCATCTGCAATGGGATTGCCCTTCACAGCCCTGGCCTGATTCCTTACTGTGCCACTTTCTTTGTTTTCACTGACTACATGAGAGCTGCCATAAGGATCTCTGCCTTGTCAGAGGCTGGAGTTATCTATGTTATGACCCACGATTCAATTGGTCTTGGAGAAGATGGACCAACCCATCAGCCGATTGAGCACTTGGCAAGTTTCCGGGCAATGCCCAACATTTTGATGCTCCGCCCAGCTGATGGGAATGAGACTGCTGGGGCATACAGGGTTGCAGTCCTTAACAGGAAAAGACCATCCATCCTTGCCCTCTCTCGACAAAAGTTGCCCAATCTTCCTGGAACTTCCATTGAGGGAGTTGAGAAGGGAGGCTACACAATATCAGACAACTCTTCCGGTAACAAGCCAGATGTCATTTTGATGGCAACTGGATCTGAGTTGGAAATTGTTGCCAAAGCTGGCGATGAGCTCAGAAAAGAAGGGAAGACTGTTAGAGTTGTCTCCTTTGTTTCTTGGGAACTCTTTGATGATCAATCAGATGACTACAAGGAAAGTGTCTTGCCGGCTGCGGTAACAGCTAGGGTTAGCCTTGAGGCTGGATCAACATTCGGGTGGCACAAGATTGTTGGAAGCAAAGGAAAGGTGATTGGAATCGATAAATTTGGGGCAAGTGCACCGGCTGGAAAAATATACAAGGAGTATGGCATCACTGTTGAGGCAGTTGTTGCTGCAGCAAAAGAAGTTAGCAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004802 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0016020 GO:0016740 GO:0016744 GO:0031976 GO:0031984 GO:0034357 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0055035

KEGG Pathways

Metabolic & Signaling

ko00030 ko00710 ko01051 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00030 map00710 map01051 map01100 map01110 map01120 map01130 map01200 map01230

Pfam Domains

Protein Families

Transket_pyr Transketolase_C Transketolase_N

Protein Analysis

748

Amino Acids

80.56

Weight (kDa)

6.29

Isoelectric Point (pI)

34.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Transketolase_N	PF00456	88 - 419	4.4e-153	Transketolase, thiamine diphosphate binding domain
Transket_pyr	PF02779	437 - 607	5.7e-53	Transketolase, pyrimidine binding domain
Transketolase_C_1	PF22613	620 - 734	8e-40	Transketolase-like TK C-terminal domain
Transketolase_C	PF02780	632 - 738	9.9e-09	Transketolase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0009245)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45290 AT2G45290 AT3G60750 AT3G60750
fragaria_vesca	FvH4_7g11510 FvH4_7g11510 FvH4_7g11510 FvH4_7g11510
malus_domestica	MD02G1207200.v1.1 MD07G1119500.v1.1
prunus_persica	Prupe.2G143600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0349951
rosa_laevigata	RLG00000028513
rosa_multiflora	Rmu_co8514097.1_g000001 Rmu_sc0000778.1_g000002 Rmu_sc0005220.1_g000014
rosa_roxburghii	Rroxscaffold_4G00305250
rosa_rugosa	Rorug01G0207300
rosa_samantha	Rh1BG191100 Rh1CG208600 Rh1DG219700
rosa_wichuraiana	Rw0G007600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	251
Acc36I	ACCTGC	1 cut(s)	1264
AccB7I	CCANNNNNTGG	1 cut(s)	1733
AccII	CGCG	2 cut(s)	178, 218
AciI	CCGC	9 cut(s)	169, 176, 181, 216, 218, 1138, 1146, 1723, 2060
AclWI	GGATC	6 cut(s)	271, 996, 1009, 1595, 1930, 2098
AcoI	YGGCCR	2 cut(s)	200, 1088
AcsI	RAATTY	2 cut(s)	1318, 2150
AcuI	CTGAAG	2 cut(s)	1170, 1250
AcvI	CACGTG	1 cut(s)	1108
AcyI	GRCGYC	1 cut(s)	248
AdeI	CACNNNGTG	1 cut(s)	572
AfaI	GTAC	4 cut(s)	368, 386, 1208, 1286
AfiI	CCNNNNNNNGG	7 cut(s)	84, 136, 286, 526, 865, 1351, 1733
AgeI	ACCGGT	2 cut(s)	420, 587
AgsI	TTSAA	7 cut(s)	412, 511, 578, 738, 1166, 1172, 1638
AjnI	CCWGG	6 cut(s)	30, 39, 541, 1070, 1522, 1828
Alw21I	GWGCWC	4 cut(s)	349, 1683, 1962, 2167
Alw26I	GTCTC	8 cut(s)	94, 218, 239, 557, 816, 1419, 1736, 1996
Alw44I	GTGCAC	1 cut(s)	2163
AlwI	GGATC	6 cut(s)	271, 996, 1009, 1595, 1930, 2098
AlwNI	CAGNNNCTG	1 cut(s)	1607
Ama87I	CYCGRG	3 cut(s)	45, 325, 1279
AoxI	GGCC	8 cut(s)	33, 42, 127, 192, 200, 309, 1088, 1525
ApaLI	GTGCAC	1 cut(s)	2163
ApoI	RAATTY	2 cut(s)	1318, 2150
ArsI	GACNNNNNNTTYG	2 cut(s)	845, 877
AsiGI	ACCGGT	2 cut(s)	420, 587
Asp700I	GAANNNNTTC	1 cut(s)	515
AspLEI	GCGC	2 cut(s)	180, 1455
AspS9I	GGNCC	6 cut(s)	34, 43, 192, 205, 590, 1658
AsuC2I	CCSGG	7 cut(s)	86, 209, 326, 327, 570, 1362, 1697
AsuHPI	GGTGA	8 cut(s)	280, 314, 722, 832, 904, 991, 1259, 2146
AvaI	CYCGRG	3 cut(s)	45, 325, 1279
AvaII	GGWCC	3 cut(s)	205, 590, 1658
BaeGI	GKGCMC	2 cut(s)	765, 2167
BaeI	ACNNNNGTAYC	2 cut(s)	475, 508
BalI	TGGCCA	1 cut(s)	1090
BamHI	GGATCC	1 cut(s)	1001
BanII	GRGCYC	1 cut(s)	1962
BarI	GAAGNNNNNNTAC	2 cut(s)	1847, 1879
BbrPI	CACGTG	1 cut(s)	1108
BbsI	GAAGAC	2 cut(s)	156, 1982
Bbv12I	GWGCWC	4 cut(s)	349, 1683, 1962, 2167
BceAI	ACGGC	2 cut(s)	79, 142
BciT130I	CCWGG	6 cut(s)	32, 41, 543, 1072, 1524, 1830
BciVI	GTATCC	1 cut(s)	476
BclI	TGATCA	1 cut(s)	2020
BcnI	CCSGG	7 cut(s)	86, 209, 326, 327, 570, 1362, 1697
BcoDI	GTCTC	8 cut(s)	94, 218, 239, 557, 816, 1419, 1736, 1996
BfaI	CTAG	1 cut(s)	2070
BfmI	CTRYAG	2 cut(s)	1221, 2220
BfuAI	ACCTGC	1 cut(s)	1264
BfuI	GTATCC	1 cut(s)	476
BglI	GCCNNNNNGGC	1 cut(s)	41
BglII	AGATCT	1 cut(s)	1384
Bme18I	GGWCC	3 cut(s)	205, 590, 1658
BmeT110I	CYCGRG	3 cut(s)	45, 325, 1279
BmgT120I	GGNCC	6 cut(s)	34, 43, 192, 205, 590, 1658
BmiI	GGNNCC	7 cut(s)	67, 193, 592, 1003, 1051, 1136, 1492
BmrI	ACTGGG	2 cut(s)	775, 918
BmsI	GCATC	3 cut(s)	1054, 1707, 2205
BmuI	ACTGGG	2 cut(s)	775, 918
BpiI	GAAGAC	2 cut(s)	156, 1982
BplI	GAGNNNNNCTC	2 cut(s)	1273, 1305
BpmI	CTGGAG	2 cut(s)	1153, 1629
BpuEI	CTTGAG	2 cut(s)	1311, 2102
BpuMI	CCSGG	7 cut(s)	86, 209, 326, 327, 570, 1362, 1697
Bsa29I	ATCGAT	1 cut(s)	2145
BsaAI	YACGTR	1 cut(s)	1108
BsaBI	GATNNNNATC	1 cut(s)	2141
BsaHI	GRCGYC	1 cut(s)	248
BsaI	GGTCTC	1 cut(s)	94
BsaWI	WCCGGW	4 cut(s)	279, 420, 587, 1886
BsaXI	ACNNNNNCTCC	8 cut(s)	50, 62, 74, 80, 92, 104, 236, 266
Bsc4I	CCNNNNNNNGG	7 cut(s)	84, 136, 286, 526, 865, 1351, 1733
Bse118I	RCCGGY	6 cut(s)	198, 202, 420, 587, 2053, 2167
Bse1I	ACTGG	5 cut(s)	410, 770, 913, 1136, 1924
Bse3DI	GCAATG	3 cut(s)	1462, 1507, 1707
Bse8I	GATNNNNATC	1 cut(s)	2141
BseBI	CCWGG	6 cut(s)	32, 41, 543, 1072, 1524, 1830
BseCI	ATCGAT	1 cut(s)	2145
BseGI	GGATG	6 cut(s)	347, 452, 1091, 1123, 1787, 1791
BseJI	GATNNNNATC	1 cut(s)	2141
BseLI	CCNNNNNNNGG	7 cut(s)	84, 136, 286, 526, 865, 1351, 1733
BseMI	GCAATG	3 cut(s)	1462, 1507, 1707
BseMII	CTCAG	6 cut(s)	543, 627, 819, 1284, 1917, 1975
BseNI	ACTGG	5 cut(s)	410, 770, 913, 1136, 1924
BseSI	GKGCMC	2 cut(s)	765, 2167
BseX3I	CGGCCG	1 cut(s)	200
BseYI	CCCAGC	3 cut(s)	1300, 1726, 1748
BsgI	GTGCAG	5 cut(s)	455, 636, 1026, 1143, 1401
Bsh1236I	CGCG	2 cut(s)	178, 218
Bsh1285I	CGRYCG	3 cut(s)	85, 203, 222
BshFI	GGCC	8 cut(s)	35, 44, 129, 194, 202, 311, 1090, 1527
BshTI	ACCGGT	2 cut(s)	420, 587
BshVI	ATCGAT	1 cut(s)	2145
BsiEI	CGRYCG	3 cut(s)	85, 203, 222
BsiHKAI	GWGCWC	4 cut(s)	349, 1683, 1962, 2167
BsiHKCI	CYCGRG	3 cut(s)	45, 325, 1279
BslFI	GGGAC	3 cut(s)	13, 576, 1282
BslI	CCNNNNNNNGG	7 cut(s)	84, 136, 286, 526, 865, 1351, 1733
BsmAI	GTCTC	8 cut(s)	94, 218, 239, 557, 816, 1419, 1736, 1996
BsmBI	CGTCTC	1 cut(s)	239
BsmFI	GGGAC	3 cut(s)	13, 576, 1282
BsmI	GAATGC	3 cut(s)	619, 836, 1183
BsnI	GGCC	8 cut(s)	35, 44, 129, 194, 202, 311, 1090, 1527
Bso31I	GGTCTC	1 cut(s)	94
BsoBI	CYCGRG	3 cut(s)	45, 325, 1279
Bsp1286I	GDGCHC	5 cut(s)	349, 765, 1683, 1962, 2167
Bsp143I	GATC	8 cut(s)	276, 678, 1001, 1384, 1587, 1922, 2020, 2090
Bsp19I	CCATGG	1 cut(s)	336
BspACI	CCGC	9 cut(s)	169, 176, 181, 216, 218, 1138, 1146, 1723, 2060
BspANI	GGCC	8 cut(s)	35, 44, 129, 194, 202, 311, 1090, 1527
BspCNI	CTCAG	6 cut(s)	544, 628, 820, 1285, 1918, 1974
BspDI	ATCGAT	1 cut(s)	2145
BspFNI	CGCG	2 cut(s)	178, 218
BspHI	TCATGA	1 cut(s)	378
BspLI	GGNNCC	7 cut(s)	67, 193, 592, 1003, 1051, 1136, 1492
BspMAI	CTGCAG	2 cut(s)	1225, 2224
BspMI	ACCTGC	1 cut(s)	1264
BspPI	GGATC	6 cut(s)	271, 996, 1009, 1595, 1930, 2098
BspQI	GCTCTTC	1 cut(s)	1443
BspTNI	GGTCTC	1 cut(s)	94
BsrDI	GCAATG	3 cut(s)	1462, 1507, 1707
BsrFI	RCCGGY	6 cut(s)	198, 202, 420, 587, 2053, 2167
BsrI	ACTGG	5 cut(s)	410, 770, 913, 1136, 1924
BssAI	RCCGGY	6 cut(s)	198, 202, 420, 587, 2053, 2167
BssMI	GATC	8 cut(s)	276, 678, 1001, 1384, 1587, 1922, 2020, 2090
BssNI	GRCGYC	1 cut(s)	248
BssT1I	CCWWGG	4 cut(s)	336, 594, 1053, 1081
Bst2UI	CCWGG	6 cut(s)	32, 41, 543, 1072, 1524, 1830
Bst4CI	ACNGT	8 cut(s)	416, 491, 674, 701, 1028, 1541, 1981, 2203
Bst6I	CTCTTC	3 cut(s)	506, 1443, 1888
BstACI	GRCGYC	1 cut(s)	248
BstAPI	GCANNNNNTGC	2 cut(s)	1388, 2216
BstBAI	YACGTR	1 cut(s)	1108
BstC8I	GCNNGC	3 cut(s)	185, 1951, 2055
BstDEI	CTNAG	7 cut(s)	552, 636, 828, 1293, 1350, 1926, 1961
BstDSI	CCRYGG	4 cut(s)	61, 216, 240, 336
BstEII	GGTNACC	1 cut(s)	320
BstENI	CCTNNNNNAGG	1 cut(s)	1349
BstF5I	GGATG	6 cut(s)	347, 452, 1091, 1123, 1787, 1791
BstFNI	CGCG	2 cut(s)	178, 218
BstHHI	GCGC	2 cut(s)	180, 1455
BstKTI	GATC	8 cut(s)	279, 681, 1004, 1387, 1590, 1925, 2023, 2093
BstMAI	GTCTC	8 cut(s)	94, 218, 239, 557, 816, 1419, 1736, 1996
BstMBI	GATC	8 cut(s)	276, 678, 1001, 1384, 1587, 1922, 2020, 2090
BstMCI	CGRYCG	3 cut(s)	85, 203, 222
BstNI	CCWGG	6 cut(s)	32, 41, 543, 1072, 1524, 1830
BstNSI	RCATGY	1 cut(s)	884
BstPI	GGTNACC	1 cut(s)	320
BstSFI	CTRYAG	2 cut(s)	1221, 2220
BstSLI	GKGCMC	2 cut(s)	765, 2167
BstUI	CGCG	2 cut(s)	178, 218
BstV2I	GAAGAC	2 cut(s)	156, 1982
BstX2I	RGATCY	4 cut(s)	1001, 1384, 1587, 1922
BstYI	RGATCY	4 cut(s)	1001, 1384, 1587, 1922
BstZI	CGGCCG	1 cut(s)	200
Bsu15I	ATCGAT	1 cut(s)	2145
BsuI	GTATCC	1 cut(s)	476
BsuRI	GGCC	8 cut(s)	35, 44, 129, 194, 202, 311, 1090, 1527
BsuTUI	ATCGAT	1 cut(s)	2145
BtgI	CCRYGG	4 cut(s)	61, 216, 240, 336
BtgZI	GCGATG	2 cut(s)	138, 1968
BtsCI	GGATG	6 cut(s)	347, 452, 1091, 1123, 1787, 1791
BtsIMutI	CAGTG	9 cut(s)	496, 679, 763, 825, 906, 1033, 1129, 1560, 2199
BveI	ACCTGC	1 cut(s)	1264
Cac8I	GCNNGC	3 cut(s)	185, 1951, 2055
CaiI	CAGNNNCTG	1 cut(s)	1607
CciI	TCATGA	1 cut(s)	378
CfoI	GCGC	2 cut(s)	180, 1455
Cfr10I	RCCGGY	6 cut(s)	198, 202, 420, 587, 2053, 2167
Cfr13I	GGNCC	6 cut(s)	34, 43, 192, 205, 590, 1658
Cfr42I	CCGCGG	1 cut(s)	219
Cfr9I	CCCGGG	1 cut(s)	325
ClaI	ATCGAT	1 cut(s)	2145
CpoI	CGGWCCG	1 cut(s)	205
CseI	GACGC	2 cut(s)	305, 1316
Csp6I	GTAC	4 cut(s)	367, 385, 1207, 1285
CspAI	ACCGGT	2 cut(s)	420, 587
CspCI	CAANNNNNGTGG	2 cut(s)	1534, 1569
CspI	CGGWCCG	1 cut(s)	205
CviAII	CATG	7 cut(s)	337, 379, 881, 1034, 1402, 1481, 1570
CviQI	GTAC	4 cut(s)	367, 385, 1207, 1285
DdeI	CTNAG	7 cut(s)	552, 636, 828, 1293, 1350, 1926, 1961
DpnI	GATC	8 cut(s)	278, 680, 1003, 1386, 1589, 1924, 2022, 2092
DpnII	GATC	8 cut(s)	276, 678, 1001, 1384, 1587, 1922, 2020, 2090
DraIII	CACNNNGTG	1 cut(s)	572
EaeI	YGGCCR	2 cut(s)	200, 1088
EagI	CGGCCG	1 cut(s)	200
Eam1104I	CTCTTC	3 cut(s)	506, 1443, 1888
EarI	CTCTTC	3 cut(s)	506, 1443, 1888
EciI	GGCGGA	1 cut(s)	1712
Ecl136II	GAGCTC	1 cut(s)	1960
EclXI	CGGCCG	1 cut(s)	200
Eco130I	CCWWGG	4 cut(s)	336, 594, 1053, 1081
Eco24I	GRGCYC	1 cut(s)	1962
Eco31I	GGTCTC	1 cut(s)	94
Eco47I	GGWCC	3 cut(s)	205, 590, 1658
Eco52I	CGGCCG	1 cut(s)	200
Eco53kI	GAGCTC	1 cut(s)	1960
Eco57I	CTGAAG	2 cut(s)	1170, 1250
Eco72I	CACGTG	1 cut(s)	1108
Eco88I	CYCGRG	3 cut(s)	45, 325, 1279
Eco91I	GGTNACC	1 cut(s)	320
EcoICRI	GAGCTC	1 cut(s)	1960
EcoNI	CCTNNNNNAGG	1 cut(s)	1349
EcoO109I	RGGNCCY	1 cut(s)	34
EcoO65I	GGTNACC	1 cut(s)	320
EcoRII	CCWGG	6 cut(s)	30, 39, 541, 1070, 1522, 1828
EcoT14I	CCWWGG	4 cut(s)	336, 594, 1053, 1081
EcoT38I	GRGCYC	1 cut(s)	1962
ErhI	CCWWGG	4 cut(s)	336, 594, 1053, 1081
Esp3I	CGTCTC	1 cut(s)	239
FaeI	CATG	7 cut(s)	340, 382, 884, 1037, 1405, 1484, 1573
FalI	AAGNNNNNCTT	2 cut(s)	1320, 1352
FaqI	GGGAC	3 cut(s)	13, 576, 1282
FatI	CATG	7 cut(s)	336, 378, 880, 1033, 1401, 1480, 1569
FauI	CCCGC	2 cut(s)	176, 183
FauNDI	CATATG	1 cut(s)	1093
FbaI	TGATCA	1 cut(s)	2020
FokI	GGATG	6 cut(s)	354, 459, 1098, 1130, 1774, 1778
FriOI	GRGCYC	1 cut(s)	1962
FspBI	CTAG	1 cut(s)	2070
GlaI	GCGC	2 cut(s)	179, 1454
GsaI	CCCAGC	3 cut(s)	1304, 1730, 1752
GsuI	CTGGAG	2 cut(s)	1153, 1629
HaeIII	GGCC	8 cut(s)	35, 44, 129, 194, 202, 311, 1090, 1527
HgaI	GACGC	2 cut(s)	305, 1316
HhaI	GCGC	2 cut(s)	180, 1455
Hin1I	GRCGYC	1 cut(s)	248
Hin1II	CATG	7 cut(s)	340, 382, 884, 1037, 1405, 1484, 1573
Hin6I	GCGC	2 cut(s)	178, 1453
HinP1I	GCGC	2 cut(s)	178, 1453
HincII	GTYRAC	2 cut(s)	685, 853
HindII	GTYRAC	2 cut(s)	685, 853
HinfI	GANTC	6 cut(s)	226, 922, 1295, 1531, 1634, 2142
HphI	GGTGA	8 cut(s)	280, 314, 722, 832, 904, 991, 1259, 2146
Hpy166II	GTNNAC	4 cut(s)	685, 853, 1287, 2165
Hpy188I	TCNGA	6 cut(s)	82, 1603, 1876, 1927, 1964, 2029
Hpy188III	TCNNGA	7 cut(s)	223, 230, 379, 1279, 1292, 1432, 1806
Hpy8I	GTNNAC	4 cut(s)	685, 853, 1287, 2165
Hpy99I	CGWCG	3 cut(s)	163, 253, 374
HpyAV	CCTTC	6 cut(s)	301, 799, 1145, 1523, 1849, 1964
HpyCH4III	ACNGT	8 cut(s)	416, 491, 674, 701, 1028, 1541, 1981, 2203
HpyCH4IV	ACGT	3 cut(s)	248, 655, 1107
HpyF3I	CTNAG	7 cut(s)	552, 636, 828, 1293, 1350, 1926, 1961
HpySE526I	ACGT	3 cut(s)	248, 655, 1107
Hsp92I	GRCGYC	1 cut(s)	248
Hsp92II	CATG	7 cut(s)	340, 382, 884, 1037, 1405, 1484, 1573
HspAI	GCGC	2 cut(s)	178, 1453
KroI	GCCGGC	1 cut(s)	2053
KroNI	GCCGGC	1 cut(s)	2055
Ksp22I	TGATCA	1 cut(s)	2020
KspI	CCGCGG	1 cut(s)	219
Kzo9I	GATC	8 cut(s)	276, 678, 1001, 1384, 1587, 1922, 2020, 2090
LguI	GCTCTTC	1 cut(s)	1443
LmnI	GCTCC	5 cut(s)	71, 352, 1134, 1490, 1725
LweI	GCATC	3 cut(s)	1054, 1707, 2205
MaeI	CTAG	1 cut(s)	2070
MaeII	ACGT	3 cut(s)	248, 655, 1107
MalI	GATC	8 cut(s)	278, 680, 1003, 1386, 1589, 1924, 2022, 2092
MboI	GATC	8 cut(s)	276, 678, 1001, 1384, 1587, 1922, 2020, 2090
MfeI	CAATTG	1 cut(s)	1638
MflI	RGATCY	4 cut(s)	1001, 1384, 1587, 1922
MhlI	GDGCHC	5 cut(s)	349, 765, 1683, 1962, 2167
MlsI	TGGCCA	1 cut(s)	1090
MluCI	AATT	5 cut(s)	313, 1318, 1638, 1935, 2150
MluNI	TGGCCA	1 cut(s)	1090
MlyI	GAGTC	2 cut(s)	220, 1304
MmeI	TCCRAC	4 cut(s)	93, 105, 1911, 2098
Mox20I	TGGCCA	1 cut(s)	1090
MroNI	GCCGGC	1 cut(s)	2053
MroXI	GAANNNNTTC	1 cut(s)	515
MscI	TGGCCA	1 cut(s)	1090
MseI	TTAA	6 cut(s)	936, 1122, 1242, 1338, 1773, 2242
MslI	CAYNNNNRTG	3 cut(s)	1715, 1910, 2101
Msp20I	TGGCCA	1 cut(s)	1090
MspA1I	CMGCKG	4 cut(s)	218, 1148, 1304, 1730
MunI	CAATTG	1 cut(s)	1638
Mva1269I	GAATGC	3 cut(s)	619, 836, 1183
MvaI	CCWGG	6 cut(s)	32, 41, 543, 1072, 1524, 1830
MvnI	CGCG	2 cut(s)	178, 218
NaeI	GCCGGC	1 cut(s)	2055
NciI	CCSGG	7 cut(s)	86, 209, 326, 327, 570, 1362, 1697
NcoI	CCATGG	1 cut(s)	336
NdeI	CATATG	1 cut(s)	1093
NdeII	GATC	8 cut(s)	276, 678, 1001, 1384, 1587, 1922, 2020, 2090
NgoMIV	GCCGGC	1 cut(s)	2053
NlaIII	CATG	7 cut(s)	340, 382, 884, 1037, 1405, 1484, 1573
NlaIV	GGNNCC	7 cut(s)	67, 193, 592, 1003, 1051, 1136, 1492
NmuCI	GTSAC	6 cut(s)	320, 416, 580, 952, 979, 1127
NspI	RCATGY	1 cut(s)	884
PagI	TCATGA	1 cut(s)	378
PciSI	GCTCTTC	1 cut(s)	1443
PcsI	WCGNNNNNNNCGW	3 cut(s)	155, 248, 272
PctI	GAATGC	3 cut(s)	619, 836, 1183
PdiI	GCCGGC	1 cut(s)	2055
PdmI	GAANNNNTTC	1 cut(s)	515
PfeI	GAWTC	4 cut(s)	922, 1531, 1634, 2142
PflFI	GACNNNGTC	1 cut(s)	86
PflMI	CCANNNNNTGG	1 cut(s)	1733
PfoI	TCCNGGA	1 cut(s)	1828
PinAI	ACCGGT	2 cut(s)	420, 587
PleI	GAGTC	2 cut(s)	220, 1303
PmaCI	CACGTG	1 cut(s)	1108
PmlI	CACGTG	1 cut(s)	1108
PpsI	GAGTC	2 cut(s)	220, 1303
Ppu21I	YACGTR	1 cut(s)	1108
Psp124BI	GAGCTC	1 cut(s)	1962
Psp6I	CCWGG	6 cut(s)	30, 39, 541, 1070, 1522, 1828
PspCI	CACGTG	1 cut(s)	1108
PspEI	GGTNACC	1 cut(s)	320
PspFI	CCCAGC	3 cut(s)	1300, 1726, 1748
PspGI	CCWGG	6 cut(s)	30, 39, 541, 1070, 1522, 1828
PspN4I	GGNNCC	7 cut(s)	67, 193, 592, 1003, 1051, 1136, 1492
PspPI	GGNCC	6 cut(s)	34, 43, 192, 205, 590, 1658
PstI	CTGCAG	2 cut(s)	1225, 2224
PstNI	CAGNNNCTG	1 cut(s)	1607
PsuI	RGATCY	4 cut(s)	1001, 1384, 1587, 1922
PsyI	GACNNNGTC	1 cut(s)	86
PvuII	CAGCTG	2 cut(s)	1304, 1730
RsaI	GTAC	4 cut(s)	368, 386, 1208, 1286
RsaNI	GTAC	4 cut(s)	367, 385, 1207, 1285
RseI	CAYNNNNRTG	3 cut(s)	1715, 1910, 2101
Rsr2I	CGGWCCG	1 cut(s)	205
RsrII	CGGWCCG	1 cut(s)	205
SacI	GAGCTC	1 cut(s)	1962
SacII	CCGCGG	1 cut(s)	219
SapI	GCTCTTC	1 cut(s)	1443
SaqAI	TTAA	6 cut(s)	936, 1122, 1242, 1338, 1773, 2242
Sau3AI	GATC	8 cut(s)	276, 678, 1001, 1384, 1587, 1922, 2020, 2090
Sau96I	GGNCC	6 cut(s)	34, 43, 192, 205, 590, 1658
SchI	GAGTC	2 cut(s)	220, 1304
SduI	GDGCHC	5 cut(s)	349, 765, 1683, 1962, 2167
SfaNI	GCATC	3 cut(s)	1054, 1707, 2205
SfcI	CTRYAG	2 cut(s)	1221, 2220
SfiI	GGCCNNNNNGGCC	1 cut(s)	41
Sfr303I	CCGCGG	1 cut(s)	219
SgrBI	CCGCGG	1 cut(s)	219
SinI	GGWCC	3 cut(s)	205, 590, 1658
SmaI	CCCGGG	1 cut(s)	327
SmiMI	CAYNNNNRTG	3 cut(s)	1715, 1910, 2101
SmlI	CTYRAG	2 cut(s)	1326, 2081
SmoI	CTYRAG	2 cut(s)	1326, 2081
Sse9I	AATT	5 cut(s)	313, 1318, 1638, 1935, 2150
SsiI	CCGC	9 cut(s)	169, 176, 181, 216, 218, 1138, 1146, 1723, 2060
SspMI	CTAG	1 cut(s)	2070
SstI	GAGCTC	1 cut(s)	1962
StyI	CCWWGG	4 cut(s)	336, 594, 1053, 1081
TaaI	ACNGT	8 cut(s)	416, 491, 674, 701, 1028, 1541, 1981, 2203
TaiI	ACGT	3 cut(s)	251, 658, 1110
TaqI	TCGA	4 cut(s)	222, 925, 1807, 2145
TasI	AATT	5 cut(s)	313, 1318, 1638, 1935, 2150
TauI	GCSGC	3 cut(s)	183, 218, 1140
TfiI	GAWTC	4 cut(s)	922, 1531, 1634, 2142
Tru1I	TTAA	6 cut(s)	936, 1122, 1242, 1338, 1773, 2242
Tru9I	TTAA	6 cut(s)	936, 1122, 1242, 1338, 1773, 2242
TscAI	CASTG	9 cut(s)	496, 679, 770, 832, 913, 1033, 1136, 1567, 2206
TseFI	GTSAC	6 cut(s)	320, 416, 580, 952, 979, 1127
Tsp45I	GTSAC	6 cut(s)	320, 416, 580, 952, 979, 1127
TspDTI	ATGAA	2 cut(s)	756, 1022
TspGWI	ACGGA	4 cut(s)	257, 459, 509, 971
TspMI	CCCGGG	1 cut(s)	325
TspRI	CASTG	9 cut(s)	496, 679, 770, 832, 913, 1033, 1136, 1567, 2206
Tth111I	GACNNNGTC	1 cut(s)	86
Van91I	CCANNNNNTGG	1 cut(s)	1733
VneI	GTGCAC	1 cut(s)	2163
VpaK11BI	GGWCC	3 cut(s)	205, 590, 1658
XagI	CCTNNNNNAGG	1 cut(s)	1349
XapI	RAATTY	2 cut(s)	1318, 2150
XceI	RCATGY	1 cut(s)	884
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1637
XmaI	CCCGGG	1 cut(s)	325
XmnI	GAANNNNTTC	1 cut(s)	515
XspI	CTAG	1 cut(s)	2070
ZraI	GACGTC	1 cut(s)	249

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.