Rorug01G0207300

Transketolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0009245
Physical Location & Seq
Forward (+)
29246311 .. 29246832
522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0207300.1

Sequence Viewer

Length: 330 bp
ATGGCAGGAACTAGTGATCAATGGACTAGGGAAGAAAACAAAGCTTTCGAGGTTGCCCTAGCGGCAAACTGGAACTGCATTGAGAAAGGGGAATGGGAGAAGATTGCTTCATCGATTCCGGGGAAATCCATTGATCAAATAAAAGCACACTTCCGGCAGCTGTTGGATGATGTAGCAGCCATCGAGGATGACCAAGTGCCCTTTCCTAGTTATGCAGATGAAGATTATCCGATCAAGGAAGTCAGAAGTAGCGTCCTGGATGGTAAAGATTGTGGTGGTGAGCCAAAAGCGGTGGAAAGACAAGCCAAGTCGGTGAAGAAAACTAAATGA

Protein Analysis

109

Amino Acids

12.16

Weight (kDa)

4.91

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 7 - 55 9e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 62, 290
AhlI ACTAGT 1 cut(s) 11
AjnI CCWGG 1 cut(s) 255
AjuI GAANNNNNNNTTGG 2 cut(s) 186, 218
AluBI AGCT 2 cut(s) 44, 160
AluI AGCT 2 cut(s) 44, 160
ApeKI GCWGC 2 cut(s) 157, 176
AsuC2I CCSGG 1 cut(s) 120
AsuHPI GGTGA 2 cut(s) 290, 325
BaeGI GKGCMC 1 cut(s) 201
BbvI GCAGC 2 cut(s) 169, 188
BccI CCATC 2 cut(s) 188, 254
BciT130I CCWGG 1 cut(s) 257
BclI TGATCA 2 cut(s) 16, 133
BcnI CCSGG 1 cut(s) 120
BcuI ACTAGT 1 cut(s) 11
BfaI CTAG 4 cut(s) 12, 27, 59, 207
BglI GCCNNNNNGGC 1 cut(s) 62
BisI GCNGC 3 cut(s) 63, 158, 177
BlsI GCNGC 3 cut(s) 64, 159, 178
Bme1390I CCNGG 2 cut(s) 120, 257
BmrFI CCNGG 2 cut(s) 120, 257
BpuMI CCSGG 1 cut(s) 120
Bsa29I ATCGAT 1 cut(s) 113
BsaJI CCNNGG 1 cut(s) 119
Bse1I ACTGG 1 cut(s) 74
BseBI CCWGG 1 cut(s) 257
BseCI ATCGAT 1 cut(s) 113
BseDI CCNNGG 1 cut(s) 119
BseGI GGATG 3 cut(s) 172, 193, 265
BseNI ACTGG 1 cut(s) 74
BseSI GKGCMC 1 cut(s) 201
BseXI GCAGC 2 cut(s) 169, 188
BshVI ATCGAT 1 cut(s) 113
BsiSI CCGG 2 cut(s) 119, 154
Bsp1286I GDGCHC 1 cut(s) 201
Bsp143I GATC 3 cut(s) 16, 133, 231
BspACI CCGC 2 cut(s) 62, 290
BspDI ATCGAT 1 cut(s) 113
BsrI ACTGG 1 cut(s) 74
BssECI CCNNGG 1 cut(s) 119
BssMI GATC 3 cut(s) 16, 133, 231
Bst2UI CCWGG 1 cut(s) 257
BstF5I GGATG 3 cut(s) 172, 193, 265
BstKTI GATC 3 cut(s) 19, 136, 234
BstMBI GATC 3 cut(s) 16, 133, 231
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstNI CCWGG 1 cut(s) 257
BstSCI CCNGG 2 cut(s) 118, 255
BstSLI GKGCMC 1 cut(s) 201
BstV1I GCAGC 2 cut(s) 169, 188
Bsu15I ATCGAT 1 cut(s) 113
BsuTUI ATCGAT 1 cut(s) 113
BtsCI GGATG 3 cut(s) 172, 193, 265
ClaI ATCGAT 1 cut(s) 113
CseI GACGC 1 cut(s) 241
CspCI CAANNNNNGTGG 2 cut(s) 273, 308
CviJI RGCY 5 cut(s) 44, 160, 179, 283, 305
CviKI_1 RGCY 5 cut(s) 44, 160, 179, 283, 305
DpnI GATC 3 cut(s) 18, 135, 233
DpnII GATC 3 cut(s) 16, 133, 231
EcoRII CCWGG 1 cut(s) 255
FaiI YATR 1 cut(s) 213
FbaI TGATCA 2 cut(s) 16, 133
Fnu4HI GCNGC 3 cut(s) 63, 158, 177
FokI GGATG 3 cut(s) 179, 200, 272
Fsp4HI GCNGC 3 cut(s) 63, 158, 177
FspBI CTAG 4 cut(s) 12, 27, 59, 207
GluI GCNGC 3 cut(s) 63, 158, 177
HapII CCGG 2 cut(s) 119, 154
HgaI GACGC 1 cut(s) 241
HindIII AAGCTT 1 cut(s) 42
HinfI GANTC 1 cut(s) 115
HpaII CCGG 2 cut(s) 119, 154
HphI GGTGA 2 cut(s) 290, 325
Hpy188I TCNGA 2 cut(s) 231, 245
HpyCH4V TGCA 2 cut(s) 78, 215
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
Ksp22I TGATCA 2 cut(s) 16, 133
Kzo9I GATC 3 cut(s) 16, 133, 231
LpnPI CCDG 5 cut(s) 55, 132, 167, 242, 269
Lsp1109I GCAGC 2 cut(s) 169, 188
MaeI CTAG 4 cut(s) 12, 27, 59, 207
MalI GATC 3 cut(s) 18, 135, 233
MboI GATC 3 cut(s) 16, 133, 231
MboII GAAGA 4 cut(s) 44, 112, 233, 328
MhlI GDGCHC 1 cut(s) 201
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 2 cut(s) 43, 178
MspA1I CMGCKG 1 cut(s) 160
MspI CCGG 2 cut(s) 119, 154
MspR9I CCNGG 2 cut(s) 120, 257
MvaI CCWGG 1 cut(s) 257
MwoI GCNNNNNNNGC 1 cut(s) 62
NciI CCSGG 1 cut(s) 120
NdeII GATC 3 cut(s) 16, 133, 231
PfeI GAWTC 1 cut(s) 115
PfoI TCCNGGA 1 cut(s) 255
PkrI GCNGC 3 cut(s) 64, 159, 178
Psp6I CCWGG 1 cut(s) 255
PspGI CCWGG 1 cut(s) 255
PvuII CAGCTG 1 cut(s) 160
SatI GCNGC 3 cut(s) 63, 158, 177
Sau3AI GATC 3 cut(s) 16, 133, 231
ScrFI CCNGG 2 cut(s) 120, 257
SduI GDGCHC 1 cut(s) 201
SetI ASST 3 cut(s) 46, 54, 162
SpeI ACTAGT 1 cut(s) 11
SsiI CCGC 2 cut(s) 62, 290
SspMI CTAG 4 cut(s) 12, 27, 59, 207
StyD4I CCNGG 2 cut(s) 118, 255
TaqI TCGA 3 cut(s) 48, 113, 183
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 1 cut(s) 115
TseI GCWGC 2 cut(s) 157, 176
TspDTI ATGAA 2 cut(s) 99, 234
XspI CTAG 4 cut(s) 12, 27, 59, 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.