MD02G1218900.v1.1

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008159
Physical Location & Seq
Forward (+)
25452464 .. 25453051
588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1218900.v1.1.491

Sequence Viewer

Length: 588 bp
ATGGCTAGTTATGGCACCGTGCAAAGGCCGTCCACCACTTCAACACCACCACCTCCACTAACTACCACTGGTGATCTCAATCAGCGGAAGGACTCAACCGGAAAAAAGCGCAACGACTTCAAGCTCTTCTGCCTCTTCAACATCCCCGCAACCCCAGAAGCAACCGCAGTTCGAATCGTCAGAAACTTTAGTTATTTCGGTTTGTACTACACACTCTTCATATTGACAATACTCTCCATAACTCTAGTGCCTCAGCGCAAGGATTCCCTAATTTTCTTGACGGTAATGATTGTCGTGGGATGCTTGTACCTCGTGCTGTTAAGGGTAGTACATGAATCTCTACTGCTGCACAAGACCGTCGAGAGGCAGGTGGTGCTGGCCTTGTTGGCCATCGGGACGATGATTGAGCTCATACTCACGGATGCAGTCGTACATTTGTTGGTGACTTTGGCTAGTGTGATACCAGTTATTTTGGTTCATGCAGTTTTTAGGGTTGGAGATGATGTTTTTGTTGAGGAGGCTGGTGGTTTTGGTGAATTCGTTCCCCTCACAACAACCGATGAACCTAAGTTGTCCGATTCAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

196

Amino Acids

21.49

Weight (kDa)

6.41

Isoelectric Point (pI)

36.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 45 - 173 4.2e-18 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 358
Acc36I ACCTGC 1 cut(s) 358
AccB1I GGYRCC 1 cut(s) 14
AciI CCGC 3 cut(s) 85, 147, 165
AcoI YGGCCR 1 cut(s) 387
AcsI RAATTY 1 cut(s) 536
AfaI GTAC 4 cut(s) 206, 308, 330, 432
AfiI CCNNNNNNNGG 2 cut(s) 24, 363
AgsI TTSAA 3 cut(s) 42, 121, 139
AluBI AGCT 2 cut(s) 124, 409
AluI AGCT 2 cut(s) 124, 409
Alw21I GWGCWC 1 cut(s) 411
AoxI GGCC 3 cut(s) 26, 378, 387
ApeKI GCWGC 1 cut(s) 346
ApoI RAATTY 1 cut(s) 536
Asp700I GAANNNNTTC 1 cut(s) 540
AspLEI GCGC 2 cut(s) 111, 258
AsuHPI GGTGA 3 cut(s) 83, 454, 545
AsuII TTCGAA 1 cut(s) 172
BalI TGGCCA 1 cut(s) 389
BanI GGYRCC 1 cut(s) 14
BanII GRGCYC 1 cut(s) 411
BauI CACGAG 1 cut(s) 311
Bbv12I GWGCWC 1 cut(s) 411
BbvCI CCTCAGC 1 cut(s) 252
BbvI GCAGC 1 cut(s) 333
BccI CCATC 1 cut(s) 398
BceAI ACGGC 1 cut(s) 13
BfaI CTAG 3 cut(s) 6, 245, 453
BfuAI ACCTGC 1 cut(s) 358
BglI GCCNNNNNGGC 1 cut(s) 386
BisI GCNGC 1 cut(s) 347
BlsI GCNGC 1 cut(s) 348
BmiI GGNNCC 1 cut(s) 16
BmsI GCATC 2 cut(s) 290, 412
Bpu10I CCTNAGC 1 cut(s) 252
Bpu14I TTCGAA 1 cut(s) 172
BsaBI GATNNNNATC 1 cut(s) 78
BsaWI WCCGGW 1 cut(s) 98
BsaXI ACNNNNNCTCC 2 cut(s) 489, 519
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 363
Bse1I ACTGG 2 cut(s) 73, 464
Bse8I GATNNNNATC 1 cut(s) 78
BseGI GGATG 3 cut(s) 141, 305, 427
BseJI GATNNNNATC 1 cut(s) 78
BseLI CCNNNNNNNGG 2 cut(s) 24, 363
BseMII CTCAG 1 cut(s) 266
BseNI ACTGG 2 cut(s) 73, 464
BseRI GAGGAG 1 cut(s) 530
BseXI GCAGC 1 cut(s) 333
BsgI GTGCAG 1 cut(s) 332
BshFI GGCC 3 cut(s) 28, 380, 389
BshNI GGYRCC 1 cut(s) 14
BsiHKAI GWGCWC 1 cut(s) 411
BsiSI CCGG 1 cut(s) 99
BslFI GGGAC 1 cut(s) 409
BslI CCNNNNNNNGG 2 cut(s) 24, 363
BsmFI GGGAC 1 cut(s) 409
BsnI GGCC 3 cut(s) 28, 380, 389
Bsp119I TTCGAA 1 cut(s) 172
Bsp1286I GDGCHC 1 cut(s) 411
Bsp143I GATC 1 cut(s) 73
BspACI CCGC 3 cut(s) 85, 147, 165
BspANI GGCC 3 cut(s) 28, 380, 389
BspCNI CTCAG 1 cut(s) 265
BspLI GGNNCC 1 cut(s) 16
BspMI ACCTGC 1 cut(s) 358
BspQI GCTCTTC 1 cut(s) 131
BspT104I TTCGAA 1 cut(s) 172
BspT107I GGYRCC 1 cut(s) 14
BsrI ACTGG 2 cut(s) 73, 464
BssMI GATC 1 cut(s) 73
BssSI CACGAG 1 cut(s) 311
Bst2BI CACGAG 1 cut(s) 311
Bst4CI ACNGT 3 cut(s) 19, 283, 358
Bst6I CTCTTC 3 cut(s) 131, 140, 221
BstAPI GCANNNNNTGC 1 cut(s) 373
BstBI TTCGAA 1 cut(s) 172
BstC8I GCNNGC 1 cut(s) 378
BstDEI CTNAG 2 cut(s) 252, 567
BstF5I GGATG 3 cut(s) 141, 305, 427
BstHHI GCGC 2 cut(s) 111, 258
BstKTI GATC 1 cut(s) 76
BstMBI GATC 1 cut(s) 73
BstMWI GCNNNNNNNGC 2 cut(s) 373, 386
BstV1I GCAGC 1 cut(s) 333
BsuRI GGCC 3 cut(s) 28, 380, 389
BtsCI GGATG 3 cut(s) 141, 305, 427
BtsIMutI CAGTG 1 cut(s) 66
BveI ACCTGC 1 cut(s) 358
Cac8I GCNNGC 1 cut(s) 378
CfoI GCGC 2 cut(s) 111, 258
Csp6I GTAC 4 cut(s) 205, 307, 329, 431
CviAII CATG 2 cut(s) 332, 479
CviJI RGCY 8 cut(s) 5, 28, 124, 380, 389, 409, 452, 521
CviKI_1 RGCY 8 cut(s) 5, 28, 124, 380, 389, 409, 452, 521
CviQI GTAC 4 cut(s) 205, 307, 329, 431
DdeI CTNAG 2 cut(s) 252, 567
DpnI GATC 1 cut(s) 75
DpnII GATC 1 cut(s) 73
EaeI YGGCCR 1 cut(s) 387
Eam1104I CTCTTC 3 cut(s) 131, 140, 221
EarI CTCTTC 3 cut(s) 131, 140, 221
Ecl136II GAGCTC 1 cut(s) 409
Eco24I GRGCYC 1 cut(s) 411
Eco53kI GAGCTC 1 cut(s) 409
EcoICRI GAGCTC 1 cut(s) 409
EcoRI GAATTC 1 cut(s) 536
EcoT38I GRGCYC 1 cut(s) 411
FaeI CATG 2 cut(s) 335, 482
FaiI YATR 6 cut(s) 12, 221, 239, 333, 413, 480
FaqI GGGAC 1 cut(s) 409
FatI CATG 2 cut(s) 331, 478
FauI CCCGC 1 cut(s) 154
Fnu4HI GCNGC 1 cut(s) 347
FokI GGATG 3 cut(s) 128, 312, 434
FriOI GRGCYC 1 cut(s) 411
Fsp4HI GCNGC 1 cut(s) 347
FspBI CTAG 3 cut(s) 6, 245, 453
GlaI GCGC 2 cut(s) 110, 257
GluI GCNGC 1 cut(s) 347
HaeIII GGCC 3 cut(s) 28, 380, 389
HapII CCGG 1 cut(s) 99
HhaI GCGC 2 cut(s) 111, 258
Hin1II CATG 2 cut(s) 335, 482
Hin6I GCGC 2 cut(s) 109, 256
HinP1I GCGC 2 cut(s) 109, 256
HinfI GANTC 5 cut(s) 92, 174, 263, 335, 578
HpaII CCGG 1 cut(s) 99
HphI GGTGA 3 cut(s) 83, 454, 545
Hpy166II GTNNAC 1 cut(s) 33
Hpy188I TCNGA 2 cut(s) 182, 577
Hpy188III TCNNGA 3 cut(s) 277, 361, 394
Hpy8I GTNNAC 1 cut(s) 33
Hpy99I CGWCG 1 cut(s) 362
HpyAV CCTTC 1 cut(s) 82
HpyCH4III ACNGT 3 cut(s) 19, 283, 358
HpyCH4V TGCA 4 cut(s) 22, 349, 425, 482
HpyF10VI GCNNNNNNNGC 2 cut(s) 373, 386
HpyF3I CTNAG 2 cut(s) 252, 567
Hsp92II CATG 2 cut(s) 335, 482
HspAI GCGC 2 cut(s) 109, 256
Kzo9I GATC 1 cut(s) 73
LguI GCTCTTC 1 cut(s) 131
LpnPI CCDG 7 cut(s) 54, 112, 168, 353, 362, 477, 507
Lsp1109I GCAGC 1 cut(s) 333
LweI GCATC 2 cut(s) 290, 412
MaeI CTAG 3 cut(s) 6, 245, 453
MaeIII GTNAC 1 cut(s) 442
MalI GATC 1 cut(s) 75
MboI GATC 1 cut(s) 73
MboII GAAGA 3 cut(s) 118, 127, 208
MhlI GDGCHC 1 cut(s) 411
MlsI TGGCCA 1 cut(s) 389
MluCI AATT 2 cut(s) 270, 536
MluNI TGGCCA 1 cut(s) 389
MlyI GAGTC 1 cut(s) 86
MmeI TCCRAC 1 cut(s) 475
MnlI CCTC 8 cut(s) 63, 143, 261, 320, 357, 508, 511, 557
Mox20I TGGCCA 1 cut(s) 389
MroXI GAANNNNTTC 1 cut(s) 540
MscI TGGCCA 1 cut(s) 389
MseI TTAA 1 cut(s) 320
Msp20I TGGCCA 1 cut(s) 389
MspA1I CMGCKG 1 cut(s) 85
MspI CCGG 1 cut(s) 99
MwoI GCNNNNNNNGC 2 cut(s) 373, 386
NdeII GATC 1 cut(s) 73
NlaIII CATG 2 cut(s) 335, 482
NlaIV GGNNCC 1 cut(s) 16
NmuCI GTSAC 1 cut(s) 442
NspV TTCGAA 1 cut(s) 172
PaqCI CACCTGC 1 cut(s) 358
PciSI GCTCTTC 1 cut(s) 131
PdmI GAANNNNTTC 1 cut(s) 540
PfeI GAWTC 4 cut(s) 174, 263, 335, 578
PkrI GCNGC 1 cut(s) 348
PleI GAGTC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 86
Psp124BI GAGCTC 1 cut(s) 411
PspN4I GGNNCC 1 cut(s) 16
RsaI GTAC 4 cut(s) 206, 308, 330, 432
RsaNI GTAC 4 cut(s) 205, 307, 329, 431
SacI GAGCTC 1 cut(s) 411
SapI GCTCTTC 1 cut(s) 131
SaqAI TTAA 1 cut(s) 320
SatI GCNGC 1 cut(s) 347
Sau3AI GATC 1 cut(s) 73
SchI GAGTC 1 cut(s) 86
SduI GDGCHC 1 cut(s) 411
SetI ASST 6 cut(s) 55, 126, 312, 372, 411, 568
SfaNI GCATC 2 cut(s) 290, 412
SfiI GGCCNNNNNGGCC 1 cut(s) 386
SfuI TTCGAA 1 cut(s) 172
Sse9I AATT 2 cut(s) 270, 536
SsiI CCGC 3 cut(s) 85, 147, 165
SspMI CTAG 3 cut(s) 6, 245, 453
SstI GAGCTC 1 cut(s) 411
TaaI ACNGT 3 cut(s) 19, 283, 358
TaqI TCGA 2 cut(s) 172, 360
TasI AATT 2 cut(s) 270, 536
TatI WGTACW 2 cut(s) 204, 328
TfiI GAWTC 4 cut(s) 174, 263, 335, 578
Tru1I TTAA 1 cut(s) 320
Tru9I TTAA 1 cut(s) 320
TscAI CASTG 1 cut(s) 73
TseFI GTSAC 1 cut(s) 442
TseI GCWGC 1 cut(s) 346
Tsp45I GTSAC 1 cut(s) 442
TspDTI ATGAA 4 cut(s) 208, 348, 467, 576
TspGWI ACGGA 1 cut(s) 434
TspRI CASTG 1 cut(s) 73
XapI RAATTY 1 cut(s) 536
XmnI GAANNNNTTC 1 cut(s) 540
XspI CTAG 3 cut(s) 6, 245, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.