pycom07g08060

PRA1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0008159
Physical Location & Seq
Reverse (-)
7449113 .. 7449691
579 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g08060.1

Sequence Viewer

Length: 579 bp
ATGGCTGACTACGGCACCATGAAAAGGCCGTCCACCATCTCCACACCAACACCTACTGATGATCCCCATAAACCGAAAGATTCAACCGGAAAAATGAGCAAGGACTTCAAGCTCTTCTGCTTCTTCAACATCCCGGCGACCCCAGAAGCCGCCGCGGTTCGCATCACCCGTAACTTTGGTTATTTCGGATCGTACTACACACTCTTGATATGGACCGTACTCTCCATAACCCTAGTGCCTCAGCGCCAGATGTCTCTAATTTTCTTGGTGATAATGACTGCAGTGGGATGCTCGTACCTTGCGCTGTTGAGGGTAGTGCCTGATTCTCTGCTGCTGCACAAGACCGTCGAAGGGCGGTTGGTGCTGGTTTTGTTGGCCATTGGGACGATGATTGAGCTCATACTTACGTATGCAGCCGAACATCTGTTTGTGACTTTAGCTTGTACGGTGCCAATTATTTTGGTTCATGCAGTTTTTAGGGTTAGAGATGATGGTTTTGTTAAGGAAGAGGATTGTGGTTTTGGTGAATATGTTCCCCCCACAACAACCGAAGAACCCAAGTTGGCGGATTCAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

193

Amino Acids

21.31

Weight (kDa)

6.07

Isoelectric Point (pI)

44.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 42 - 170 2.5e-18 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 14, 448
AccII CGCG 1 cut(s) 155
AciI CCGC 5 cut(s) 150, 153, 155, 355, 566
AclWI GGATC 2 cut(s) 56, 196
AcoI YGGCCR 1 cut(s) 375
AfaI GTAC 4 cut(s) 194, 219, 296, 445
AfiI CCNNNNNNNGG 2 cut(s) 24, 351
AgsI TTSAA 3 cut(s) 84, 109, 127
AluBI AGCT 3 cut(s) 112, 397, 440
AluI AGCT 3 cut(s) 112, 397, 440
Alw21I GWGCWC 1 cut(s) 399
Alw26I GTCTC 1 cut(s) 258
AlwI GGATC 2 cut(s) 56, 196
AoxI GGCC 2 cut(s) 26, 375
ApeKI GCWGC 3 cut(s) 331, 334, 413
Asp700I GAANNNNTTC 1 cut(s) 531
AspLEI GCGC 2 cut(s) 246, 304
AspS9I GGNCC 1 cut(s) 213
AsuC2I CCSGG 1 cut(s) 134
AsuHPI GGTGA 3 cut(s) 157, 280, 536
AvaII GGWCC 1 cut(s) 213
BalI TGGCCA 1 cut(s) 377
BanI GGYRCC 2 cut(s) 14, 448
BanII GRGCYC 1 cut(s) 399
Bbv12I GWGCWC 1 cut(s) 399
BbvCI CCTCAGC 1 cut(s) 240
BbvI GCAGC 3 cut(s) 318, 321, 425
BccI CCATC 2 cut(s) 44, 485
BceAI ACGGC 2 cut(s) 13, 28
BcnI CCSGG 1 cut(s) 134
BcoDI GTCTC 1 cut(s) 258
BfaI CTAG 1 cut(s) 233
BfmI CTRYAG 1 cut(s) 279
BfoI RGCGCY 1 cut(s) 247
BisI GCNGC 5 cut(s) 150, 153, 332, 335, 414
BlsI GCNGC 5 cut(s) 151, 154, 333, 336, 415
Bme1390I CCNGG 1 cut(s) 134
Bme18I GGWCC 1 cut(s) 213
BmgT120I GGNCC 1 cut(s) 213
BmiI GGNNCC 2 cut(s) 16, 450
BmrFI CCNGG 1 cut(s) 134
BmsI GCATC 2 cut(s) 171, 278
Bpu10I CCTNAGC 1 cut(s) 240
BpuMI CCSGG 1 cut(s) 134
BsaAI YACGTR 1 cut(s) 408
BsaJI CCNNGG 1 cut(s) 153
BsaWI WCCGGW 1 cut(s) 86
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 351
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 2 cut(s) 129, 293
BseLI CCNNNNNNNGG 2 cut(s) 24, 351
BseMII CTCAG 1 cut(s) 254
BseXI GCAGC 3 cut(s) 318, 321, 425
BsgI GTGCAG 1 cut(s) 320
Bsh1236I CGCG 1 cut(s) 155
BshFI GGCC 2 cut(s) 28, 377
BshNI GGYRCC 2 cut(s) 14, 448
BsiHKAI GWGCWC 1 cut(s) 399
BsiSI CCGG 2 cut(s) 87, 134
BslFI GGGAC 1 cut(s) 397
BslI CCNNNNNNNGG 2 cut(s) 24, 351
BsmAI GTCTC 1 cut(s) 258
BsmFI GGGAC 1 cut(s) 397
BsnI GGCC 2 cut(s) 28, 377
Bsp1286I GDGCHC 1 cut(s) 399
Bsp143I GATC 2 cut(s) 61, 188
BspACI CCGC 5 cut(s) 150, 153, 155, 355, 566
BspANI GGCC 2 cut(s) 28, 377
BspCNI CTCAG 1 cut(s) 253
BspFNI CGCG 1 cut(s) 155
BspLI GGNNCC 2 cut(s) 16, 450
BspMAI CTGCAG 1 cut(s) 283
BspPI GGATC 2 cut(s) 56, 196
BspQI GCTCTTC 1 cut(s) 119
BspT107I GGYRCC 2 cut(s) 14, 448
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 2 cut(s) 61, 188
Bst4CI ACNGT 3 cut(s) 217, 346, 448
Bst6I CTCTTC 2 cut(s) 119, 501
BstBAI YACGTR 1 cut(s) 408
BstDEI CTNAG 1 cut(s) 240
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 2 cut(s) 129, 293
BstFNI CGCG 1 cut(s) 155
BstH2I RGCGCY 1 cut(s) 247
BstHHI GCGC 2 cut(s) 246, 304
BstKTI GATC 2 cut(s) 64, 191
BstMAI GTCTC 1 cut(s) 258
BstMBI GATC 2 cut(s) 61, 188
BstMWI GCNNNNNNNGC 1 cut(s) 361
BstSCI CCNGG 1 cut(s) 132
BstSFI CTRYAG 1 cut(s) 279
BstSNI TACGTA 1 cut(s) 408
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 3 cut(s) 318, 321, 425
BsuRI GGCC 2 cut(s) 28, 377
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 2 cut(s) 129, 293
BtsI GCAGTG 1 cut(s) 288
BtsIMutI CAGTG 1 cut(s) 288
CfoI GCGC 2 cut(s) 246, 304
Cfr13I GGNCC 1 cut(s) 213
Cfr42I CCGCGG 1 cut(s) 156
Csp6I GTAC 4 cut(s) 193, 218, 295, 444
CviAII CATG 2 cut(s) 19, 467
CviJI RGCY 8 cut(s) 5, 28, 112, 149, 377, 397, 416, 440
CviKI_1 RGCY 8 cut(s) 5, 28, 112, 149, 377, 397, 416, 440
CviQI GTAC 4 cut(s) 193, 218, 295, 444
DdeI CTNAG 1 cut(s) 240
DpnI GATC 2 cut(s) 63, 190
DpnII GATC 2 cut(s) 61, 188
EaeI YGGCCR 1 cut(s) 375
Eam1104I CTCTTC 2 cut(s) 119, 501
EarI CTCTTC 2 cut(s) 119, 501
Ecl136II GAGCTC 1 cut(s) 397
Eco105I TACGTA 1 cut(s) 408
Eco24I GRGCYC 1 cut(s) 399
Eco47I GGWCC 1 cut(s) 213
Eco53kI GAGCTC 1 cut(s) 397
EcoICRI GAGCTC 1 cut(s) 397
EcoT38I GRGCYC 1 cut(s) 399
FaeI CATG 2 cut(s) 22, 470
FaiI YATR 8 cut(s) 20, 69, 211, 227, 401, 411, 468, 531
FaqI GGGAC 1 cut(s) 397
FatI CATG 2 cut(s) 18, 466
Fnu4HI GCNGC 5 cut(s) 150, 153, 332, 335, 414
FokI GGATG 2 cut(s) 116, 300
FriOI GRGCYC 1 cut(s) 399
Fsp4HI GCNGC 5 cut(s) 150, 153, 332, 335, 414
FspBI CTAG 1 cut(s) 233
GlaI GCGC 2 cut(s) 245, 303
GluI GCNGC 5 cut(s) 150, 153, 332, 335, 414
HaeII RGCGCY 1 cut(s) 247
HaeIII GGCC 2 cut(s) 28, 377
HapII CCGG 2 cut(s) 87, 134
HhaI GCGC 2 cut(s) 246, 304
Hin1II CATG 2 cut(s) 22, 470
Hin6I GCGC 2 cut(s) 244, 302
HinP1I GCGC 2 cut(s) 244, 302
HinfI GANTC 3 cut(s) 80, 323, 569
HpaII CCGG 2 cut(s) 87, 134
HphI GGTGA 3 cut(s) 157, 280, 536
Hpy166II GTNNAC 1 cut(s) 33
Hpy188I TCNGA 1 cut(s) 188
Hpy188III TCNNGA 1 cut(s) 205
Hpy8I GTNNAC 1 cut(s) 33
Hpy99I CGWCG 1 cut(s) 350
HpyAV CCTTC 1 cut(s) 344
HpyCH4III ACNGT 3 cut(s) 217, 346, 448
HpyCH4IV ACGT 1 cut(s) 407
HpyCH4V TGCA 4 cut(s) 281, 337, 413, 470
HpyF10VI GCNNNNNNNGC 1 cut(s) 361
HpyF3I CTNAG 1 cut(s) 240
HpySE526I ACGT 1 cut(s) 407
Hsp92II CATG 2 cut(s) 22, 470
HspAI GCGC 2 cut(s) 244, 302
KspI CCGCGG 1 cut(s) 156
Kzo9I GATC 2 cut(s) 61, 188
LguI GCTCTTC 1 cut(s) 119
LpnPI CCDG 6 cut(s) 100, 147, 156, 260, 333, 350
Lsp1109I GCAGC 3 cut(s) 318, 321, 425
LweI GCATC 2 cut(s) 171, 278
MaeI CTAG 1 cut(s) 233
MaeII ACGT 1 cut(s) 407
MaeIII GTNAC 2 cut(s) 170, 430
MalI GATC 2 cut(s) 63, 190
MboI GATC 2 cut(s) 61, 188
MboII GAAGA 4 cut(s) 106, 115, 518, 563
MhlI GDGCHC 1 cut(s) 399
MlsI TGGCCA 1 cut(s) 377
MluCI AATT 2 cut(s) 258, 453
MluNI TGGCCA 1 cut(s) 377
MnlI CCTC 3 cut(s) 249, 303, 502
Mox20I TGGCCA 1 cut(s) 377
MroXI GAANNNNTTC 1 cut(s) 531
MscI TGGCCA 1 cut(s) 377
MseI TTAA 1 cut(s) 501
Msp20I TGGCCA 1 cut(s) 377
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 2 cut(s) 87, 134
MspR9I CCNGG 1 cut(s) 134
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 1 cut(s) 361
NciI CCSGG 1 cut(s) 134
NdeII GATC 2 cut(s) 61, 188
NlaIII CATG 2 cut(s) 22, 470
NlaIV GGNNCC 2 cut(s) 16, 450
NmuCI GTSAC 1 cut(s) 430
PciSI GCTCTTC 1 cut(s) 119
PcsI WCGNNNNNNNCGW 1 cut(s) 166
PdmI GAANNNNTTC 1 cut(s) 531
PfeI GAWTC 3 cut(s) 80, 323, 569
PkrI GCNGC 5 cut(s) 151, 154, 333, 336, 415
Ppu21I YACGTR 1 cut(s) 408
Psp124BI GAGCTC 1 cut(s) 399
PspN4I GGNNCC 2 cut(s) 16, 450
PspPI GGNCC 1 cut(s) 213
PstI CTGCAG 1 cut(s) 283
RsaI GTAC 4 cut(s) 194, 219, 296, 445
RsaNI GTAC 4 cut(s) 193, 218, 295, 444
SacI GAGCTC 1 cut(s) 399
SacII CCGCGG 1 cut(s) 156
SapI GCTCTTC 1 cut(s) 119
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 5 cut(s) 150, 153, 332, 335, 414
Sau3AI GATC 2 cut(s) 61, 188
Sau96I GGNCC 1 cut(s) 213
ScrFI CCNGG 1 cut(s) 134
SduI GDGCHC 1 cut(s) 399
SetI ASST 6 cut(s) 55, 114, 300, 399, 410, 442
SfaNI GCATC 2 cut(s) 171, 278
SfcI CTRYAG 1 cut(s) 279
Sfr303I CCGCGG 1 cut(s) 156
SgrBI CCGCGG 1 cut(s) 156
SinI GGWCC 1 cut(s) 213
SnaBI TACGTA 1 cut(s) 408
Sse9I AATT 2 cut(s) 258, 453
SsiI CCGC 5 cut(s) 150, 153, 155, 355, 566
SspMI CTAG 1 cut(s) 233
SstI GAGCTC 1 cut(s) 399
StyD4I CCNGG 1 cut(s) 132
TaaI ACNGT 3 cut(s) 217, 346, 448
TaiI ACGT 1 cut(s) 410
TaqI TCGA 1 cut(s) 348
TasI AATT 2 cut(s) 258, 453
TauI GCSGC 2 cut(s) 152, 155
TfiI GAWTC 3 cut(s) 80, 323, 569
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TscAI CASTG 1 cut(s) 288
TseFI GTSAC 1 cut(s) 430
TseI GCWGC 3 cut(s) 331, 334, 413
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 2 cut(s) 35, 455
TspRI CASTG 1 cut(s) 288
VpaK11BI GGWCC 1 cut(s) 213
XmnI GAANNNNTTC 1 cut(s) 531
XspI CTAG 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.