MD07G1098000.v1.1

PRA1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008159
Physical Location & Seq
Reverse (-)
10793607 .. 10794185
579 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1098000.v1.1.491

Sequence Viewer

Length: 579 bp
ATGGCTGACTACGGCACCATGAAAAGGCCGTCCACCATCTCCACACCAACACCTACTGATGATCCCCATAAACCGAAAGATTCAACCGGAAAAATGAGCAAGGACTTCAAGCTCTTCTGCTTCTTCAACATCCCGGCAACCCCAGAAGCTGCCGCGGTTCGCTTCACCCGAAACTTTGGATACTTCGGATCGTACTACACACTCTTCATATGGATCGTACTCTCCATAACCCTAGTGCCTCAGCGCCGGATGTCTCTAATTTTCTTGGTGATAATGACGGCAGCGGGATGCTCGTACCTTGCGCTGTTGAGGGTAGTACCTGATTCTCTGCTGCTGCACAAGACCGTCGAAAGGCGTTTGATGCTGGTTTTGTTGGCCATTGGGACGATGATTGAGCTCATACTTACGGATGCAGCCGAATATCTGTTTGTGACTTTAGCTTGTACGGTGCCAATTATTTTGGTTCATGCAGTTTTTAGGGTTAGAGATGATGGTTTTGTTGAGGAAGAGGCTTGTGGTTTTGGTGAATATGTTCCCCCCACAACAACCGAAGAACCCAAGTTGGCGGATTCAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

193

Amino Acids

21.45

Weight (kDa)

5.92

Isoelectric Point (pI)

53.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 42 - 170 4.2e-19 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 14, 448
AccII CGCG 1 cut(s) 155
AciI CCGC 4 cut(s) 153, 155, 284, 566
AclWI GGATC 3 cut(s) 56, 196, 221
AcoI YGGCCR 1 cut(s) 375
AfaI GTAC 5 cut(s) 194, 219, 296, 318, 445
AfiI CCNNNNNNNGG 2 cut(s) 24, 351
AgsI TTSAA 3 cut(s) 84, 109, 127
AluBI AGCT 4 cut(s) 112, 149, 397, 440
AluI AGCT 4 cut(s) 112, 149, 397, 440
Alw21I GWGCWC 1 cut(s) 399
Alw26I GTCTC 1 cut(s) 258
AlwI GGATC 3 cut(s) 56, 196, 221
AlwNI CAGNNNCTG 1 cut(s) 149
AoxI GGCC 2 cut(s) 26, 375
ApeKI GCWGC 5 cut(s) 149, 281, 331, 334, 413
Asp700I GAANNNNTTC 1 cut(s) 531
AspLEI GCGC 2 cut(s) 246, 304
AsuC2I CCSGG 1 cut(s) 134
AsuHPI GGTGA 3 cut(s) 157, 280, 536
BalI TGGCCA 1 cut(s) 377
BanI GGYRCC 2 cut(s) 14, 448
BanII GRGCYC 1 cut(s) 399
Bbv12I GWGCWC 1 cut(s) 399
BbvCI CCTCAGC 1 cut(s) 240
BbvI GCAGC 5 cut(s) 136, 293, 318, 321, 425
BccI CCATC 2 cut(s) 44, 485
BceAI ACGGC 3 cut(s) 13, 28, 294
BciVI GTATCC 1 cut(s) 173
BcnI CCSGG 1 cut(s) 134
BcoDI GTCTC 1 cut(s) 258
BfaI CTAG 1 cut(s) 233
BfoI RGCGCY 1 cut(s) 247
BfuI GTATCC 1 cut(s) 173
BisI GCNGC 6 cut(s) 150, 153, 282, 332, 335, 414
BlsI GCNGC 6 cut(s) 151, 154, 283, 333, 336, 415
Bme1390I CCNGG 1 cut(s) 134
BmiI GGNNCC 2 cut(s) 16, 450
BmrFI CCNGG 1 cut(s) 134
BmsI GCATC 3 cut(s) 278, 351, 400
Bpu10I CCTNAGC 1 cut(s) 240
BpuMI CCSGG 1 cut(s) 134
BsaJI CCNNGG 1 cut(s) 153
BsaWI WCCGGW 1 cut(s) 86
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 351
BseDI CCNNGG 1 cut(s) 153
BseGI GGATG 4 cut(s) 129, 255, 293, 415
BseLI CCNNNNNNNGG 2 cut(s) 24, 351
BseMII CTCAG 1 cut(s) 254
BseXI GCAGC 5 cut(s) 136, 293, 318, 321, 425
BsgI GTGCAG 1 cut(s) 320
Bsh1236I CGCG 1 cut(s) 155
BshFI GGCC 2 cut(s) 28, 377
BshNI GGYRCC 2 cut(s) 14, 448
BsiHKAI GWGCWC 1 cut(s) 399
BsiSI CCGG 3 cut(s) 87, 134, 247
BslFI GGGAC 1 cut(s) 397
BslI CCNNNNNNNGG 2 cut(s) 24, 351
BsmAI GTCTC 1 cut(s) 258
BsmFI GGGAC 1 cut(s) 397
BsnI GGCC 2 cut(s) 28, 377
Bsp1286I GDGCHC 1 cut(s) 399
Bsp143I GATC 3 cut(s) 61, 188, 213
BspACI CCGC 4 cut(s) 153, 155, 284, 566
BspANI GGCC 2 cut(s) 28, 377
BspCNI CTCAG 1 cut(s) 253
BspFNI CGCG 1 cut(s) 155
BspLI GGNNCC 2 cut(s) 16, 450
BspPI GGATC 3 cut(s) 56, 196, 221
BspQI GCTCTTC 1 cut(s) 119
BspT107I GGYRCC 2 cut(s) 14, 448
BssECI CCNNGG 1 cut(s) 153
BssMI GATC 3 cut(s) 61, 188, 213
Bst4CI ACNGT 2 cut(s) 346, 448
Bst6I CTCTTC 3 cut(s) 119, 209, 501
BstDEI CTNAG 1 cut(s) 240
BstDSI CCRYGG 1 cut(s) 153
BstF5I GGATG 4 cut(s) 129, 255, 293, 415
BstFNI CGCG 1 cut(s) 155
BstH2I RGCGCY 1 cut(s) 247
BstHHI GCGC 2 cut(s) 246, 304
BstKTI GATC 3 cut(s) 64, 191, 216
BstMAI GTCTC 1 cut(s) 258
BstMBI GATC 3 cut(s) 61, 188, 213
BstMWI GCNNNNNNNGC 1 cut(s) 361
BstSCI CCNGG 1 cut(s) 132
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 5 cut(s) 136, 293, 318, 321, 425
BsuI GTATCC 1 cut(s) 173
BsuRI GGCC 2 cut(s) 28, 377
BtgI CCRYGG 1 cut(s) 153
BtsCI GGATG 4 cut(s) 129, 255, 293, 415
CaiI CAGNNNCTG 1 cut(s) 149
CfoI GCGC 2 cut(s) 246, 304
Cfr42I CCGCGG 1 cut(s) 156
Csp6I GTAC 5 cut(s) 193, 218, 295, 317, 444
CviAII CATG 2 cut(s) 19, 467
CviJI RGCY 9 cut(s) 5, 28, 112, 149, 377, 397, 416, 440, 512
CviKI_1 RGCY 9 cut(s) 5, 28, 112, 149, 377, 397, 416, 440, 512
CviQI GTAC 5 cut(s) 193, 218, 295, 317, 444
DdeI CTNAG 1 cut(s) 240
DpnI GATC 3 cut(s) 63, 190, 215
DpnII GATC 3 cut(s) 61, 188, 213
EaeI YGGCCR 1 cut(s) 375
Eam1104I CTCTTC 3 cut(s) 119, 209, 501
EarI CTCTTC 3 cut(s) 119, 209, 501
Ecl136II GAGCTC 1 cut(s) 397
Eco24I GRGCYC 1 cut(s) 399
Eco53kI GAGCTC 1 cut(s) 397
EcoICRI GAGCTC 1 cut(s) 397
EcoT38I GRGCYC 1 cut(s) 399
FaeI CATG 2 cut(s) 22, 470
FaiI YATR 8 cut(s) 20, 69, 209, 211, 227, 401, 468, 531
FaqI GGGAC 1 cut(s) 397
FatI CATG 2 cut(s) 18, 466
FauI CCCGC 1 cut(s) 277
FauNDI CATATG 1 cut(s) 209
Fnu4HI GCNGC 6 cut(s) 150, 153, 282, 332, 335, 414
FokI GGATG 4 cut(s) 116, 262, 300, 422
FriOI GRGCYC 1 cut(s) 399
Fsp4HI GCNGC 6 cut(s) 150, 153, 282, 332, 335, 414
FspBI CTAG 1 cut(s) 233
GlaI GCGC 2 cut(s) 245, 303
GluI GCNGC 6 cut(s) 150, 153, 282, 332, 335, 414
HaeII RGCGCY 1 cut(s) 247
HaeIII GGCC 2 cut(s) 28, 377
HapII CCGG 3 cut(s) 87, 134, 247
HhaI GCGC 2 cut(s) 246, 304
Hin1II CATG 2 cut(s) 22, 470
Hin6I GCGC 2 cut(s) 244, 302
HinP1I GCGC 2 cut(s) 244, 302
HinfI GANTC 3 cut(s) 80, 323, 569
HpaII CCGG 3 cut(s) 87, 134, 247
HphI GGTGA 3 cut(s) 157, 280, 536
Hpy166II GTNNAC 1 cut(s) 33
Hpy188I TCNGA 1 cut(s) 188
Hpy8I GTNNAC 1 cut(s) 33
Hpy99I CGWCG 1 cut(s) 350
HpyCH4III ACNGT 2 cut(s) 346, 448
HpyCH4V TGCA 3 cut(s) 337, 413, 470
HpyF10VI GCNNNNNNNGC 1 cut(s) 361
HpyF3I CTNAG 1 cut(s) 240
Hsp92II CATG 2 cut(s) 22, 470
HspAI GCGC 2 cut(s) 244, 302
KspI CCGCGG 1 cut(s) 156
Kzo9I GATC 3 cut(s) 61, 188, 213
LguI GCTCTTC 1 cut(s) 119
LpnPI CCDG 6 cut(s) 100, 147, 156, 260, 333, 350
Lsp1109I GCAGC 5 cut(s) 136, 293, 318, 321, 425
LweI GCATC 3 cut(s) 278, 351, 400
MaeI CTAG 1 cut(s) 233
MaeIII GTNAC 1 cut(s) 430
MalI GATC 3 cut(s) 63, 190, 215
MboI GATC 3 cut(s) 61, 188, 213
MboII GAAGA 5 cut(s) 106, 115, 196, 518, 563
MhlI GDGCHC 1 cut(s) 399
MlsI TGGCCA 1 cut(s) 377
MluCI AATT 2 cut(s) 258, 453
MluNI TGGCCA 1 cut(s) 377
MnlI CCTC 4 cut(s) 249, 303, 496, 502
Mox20I TGGCCA 1 cut(s) 377
MroXI GAANNNNTTC 1 cut(s) 531
MscI TGGCCA 1 cut(s) 377
Msp20I TGGCCA 1 cut(s) 377
MspA1I CMGCKG 2 cut(s) 155, 284
MspI CCGG 3 cut(s) 87, 134, 247
MspR9I CCNGG 1 cut(s) 134
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 1 cut(s) 361
NciI CCSGG 1 cut(s) 134
NdeI CATATG 1 cut(s) 209
NdeII GATC 3 cut(s) 61, 188, 213
NlaIII CATG 2 cut(s) 22, 470
NlaIV GGNNCC 2 cut(s) 16, 450
NmuCI GTSAC 1 cut(s) 430
PciSI GCTCTTC 1 cut(s) 119
PcsI WCGNNNNNNNCGW 1 cut(s) 166
PdmI GAANNNNTTC 1 cut(s) 531
PfeI GAWTC 3 cut(s) 80, 323, 569
PkrI GCNGC 6 cut(s) 151, 154, 283, 333, 336, 415
Psp124BI GAGCTC 1 cut(s) 399
PspN4I GGNNCC 2 cut(s) 16, 450
PstNI CAGNNNCTG 1 cut(s) 149
RsaI GTAC 5 cut(s) 194, 219, 296, 318, 445
RsaNI GTAC 5 cut(s) 193, 218, 295, 317, 444
SacI GAGCTC 1 cut(s) 399
SacII CCGCGG 1 cut(s) 156
SapI GCTCTTC 1 cut(s) 119
SatI GCNGC 6 cut(s) 150, 153, 282, 332, 335, 414
Sau3AI GATC 3 cut(s) 61, 188, 213
ScrFI CCNGG 1 cut(s) 134
SduI GDGCHC 1 cut(s) 399
SetI ASST 7 cut(s) 55, 114, 151, 300, 322, 399, 442
SfaNI GCATC 3 cut(s) 278, 351, 400
Sfr303I CCGCGG 1 cut(s) 156
SgrBI CCGCGG 1 cut(s) 156
Sse9I AATT 2 cut(s) 258, 453
SsiI CCGC 4 cut(s) 153, 155, 284, 566
SspMI CTAG 1 cut(s) 233
SstI GAGCTC 1 cut(s) 399
StyD4I CCNGG 1 cut(s) 132
TaaI ACNGT 2 cut(s) 346, 448
TaqI TCGA 1 cut(s) 348
TasI AATT 2 cut(s) 258, 453
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 3 cut(s) 80, 323, 569
TseFI GTSAC 1 cut(s) 430
TseI GCWGC 5 cut(s) 149, 281, 331, 334, 413
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 3 cut(s) 35, 196, 455
TspGWI ACGGA 1 cut(s) 422
XmnI GAANNNNTTC 1 cut(s) 531
XspI CTAG 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.