Rroxscaffold_4G00308940

PRA1 family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0008159
Physical Location & Seq
Forward (+)
30536940 .. 30537530
591 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00308940.1

Sequence Viewer

Length: 591 bp
ATGGCTAGCCATGGCACCGTGCAAAGACCTTCCACCACCTCAACATCCCCAACTCTCCCAACAACTCATCCCCATGAACCGAAAGACTCAACACCAAGAGTGAGACTCGAATTCAGCCTCTGTTTTCCCTTCAACATCCCCAAGACCCCAGAAGCAGCCGCGGCCCGAATCATCAGAAACTTGAGTTACTACAGATTGTACTACACACTTTTCATATGGATCATACTCTTCATAACCCTCTTGCCGAAGCGTAAGCTCTCGTTGATCTTCTTGATAGCAATGACTGCTCTGACCTGCTCGTTCCTTGCGGTGATGAGGTTAGTACCTGCTGATTCTGTTGTCCTGCAATACAAGATCATTGATAGGAGACTGGTCCTGGCTTTGCTGGTAATTGCGACCATGGTTGAGCTCATAGTCACACGTGCGGCCATACATCTGTTTGTGACTTTGGCTTGTGCGACTCCAGTGATTTTGGTGCATGCAGTTTTCAGGGTTAGAGATGATCTTTGTGTTGAAGAGGAGGCTTCTGCTGCTGGTGAATTGGTTCCACTCATAAAAAACACTGAACCCAAGTTATCAGATTCCGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

196

Amino Acids

21.89

Weight (kDa)

9.18

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 43 - 174 1.6e-21 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 302, 334
AccB1I GGYRCC 1 cut(s) 14
AccII CGCG 1 cut(s) 161
AciI CCGC 4 cut(s) 159, 161, 308, 425
AclWI GGATC 1 cut(s) 227
AcoI YGGCCR 1 cut(s) 426
AcsI RAATTY 1 cut(s) 110
AcvI CACGTG 1 cut(s) 422
AfaI GTAC 2 cut(s) 200, 324
AflIII ACRYGT 1 cut(s) 419
AgsI TTSAA 2 cut(s) 133, 515
AjnI CCWGG 1 cut(s) 375
AluBI AGCT 2 cut(s) 256, 409
AluI AGCT 2 cut(s) 256, 409
Alw21I GWGCWC 1 cut(s) 411
Alw26I GTCTC 2 cut(s) 97, 361
AlwI GGATC 1 cut(s) 227
AlwNI CAGNNNCTG 1 cut(s) 120
AoxI GGCC 2 cut(s) 162, 426
ApeKI GCWGC 2 cut(s) 155, 530
ApoI RAATTY 1 cut(s) 110
Asp700I GAANNNNTTC 1 cut(s) 543
AspS9I GGNCC 2 cut(s) 163, 373
AsuHPI GGTGA 2 cut(s) 322, 548
AsuNHI GCTAGC 1 cut(s) 5
AvaII GGWCC 1 cut(s) 373
BanI GGYRCC 1 cut(s) 14
BanII GRGCYC 1 cut(s) 411
BbrPI CACGTG 1 cut(s) 422
Bbv12I GWGCWC 1 cut(s) 411
BbvI GCAGC 2 cut(s) 167, 517
BciT130I CCWGG 1 cut(s) 377
BcoDI GTCTC 2 cut(s) 97, 361
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 190
BfuAI ACCTGC 2 cut(s) 302, 334
BisI GCNGC 5 cut(s) 156, 159, 162, 426, 531
BlsI GCNGC 5 cut(s) 157, 160, 163, 427, 532
Bme1390I CCNGG 1 cut(s) 377
Bme18I GGWCC 1 cut(s) 373
BmgT120I GGNCC 2 cut(s) 163, 373
BmiI GGNNCC 2 cut(s) 16, 546
BmrFI CCNGG 1 cut(s) 377
BmtI GCTAGC 1 cut(s) 9
BplI GAGNNNNNCTC 2 cut(s) 90, 122
BpmI CTGGAG 1 cut(s) 447
BpuEI CTTGAG 1 cut(s) 202
BsaAI YACGTR 1 cut(s) 422
BsaJI CCNNGG 3 cut(s) 10, 159, 399
Bse1I ACTGG 2 cut(s) 375, 464
Bse3DI GCAATG 1 cut(s) 285
BseBI CCWGG 1 cut(s) 377
BseDI CCNNGG 3 cut(s) 10, 159, 399
BseGI GGATG 3 cut(s) 44, 67, 135
BseMI GCAATG 1 cut(s) 285
BseNI ACTGG 2 cut(s) 375, 464
BseRI GAGGAG 1 cut(s) 533
BseXI GCAGC 2 cut(s) 167, 517
Bsh1236I CGCG 1 cut(s) 161
BshFI GGCC 2 cut(s) 164, 428
BshNI GGYRCC 1 cut(s) 14
BsiHKAI GWGCWC 1 cut(s) 411
BsmAI GTCTC 2 cut(s) 97, 361
BsnI GGCC 2 cut(s) 164, 428
Bsp1286I GDGCHC 1 cut(s) 411
Bsp143I GATC 4 cut(s) 219, 264, 354, 502
Bsp19I CCATGG 2 cut(s) 10, 399
BspACI CCGC 4 cut(s) 159, 161, 308, 425
BspANI GGCC 2 cut(s) 164, 428
BspFNI CGCG 1 cut(s) 161
BspLI GGNNCC 2 cut(s) 16, 546
BspMI ACCTGC 2 cut(s) 302, 334
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 1 cut(s) 227
BspT107I GGYRCC 1 cut(s) 14
BsrDI GCAATG 1 cut(s) 285
BsrI ACTGG 2 cut(s) 375, 464
BssECI CCNNGG 3 cut(s) 10, 159, 399
BssMI GATC 4 cut(s) 219, 264, 354, 502
BssT1I CCWWGG 2 cut(s) 10, 399
Bst2UI CCWGG 1 cut(s) 377
Bst4CI ACNGT 1 cut(s) 19
Bst6I CTCTTC 2 cut(s) 233, 510
BstAPI GCANNNNNTGC 1 cut(s) 284
BstBAI YACGTR 1 cut(s) 422
BstC8I GCNNGC 2 cut(s) 7, 480
BstDSI CCRYGG 3 cut(s) 10, 159, 399
BstF5I GGATG 3 cut(s) 44, 67, 135
BstFNI CGCG 1 cut(s) 161
BstKTI GATC 4 cut(s) 222, 267, 357, 505
BstMAI GTCTC 2 cut(s) 97, 361
BstMBI GATC 4 cut(s) 219, 264, 354, 502
BstMWI GCNNNNNNNGC 3 cut(s) 161, 284, 530
BstNI CCWGG 1 cut(s) 377
BstNSI RCATGY 1 cut(s) 482
BstSCI CCNGG 1 cut(s) 375
BstSFI CTRYAG 1 cut(s) 190
BstUI CGCG 1 cut(s) 161
BstV1I GCAGC 2 cut(s) 167, 517
BsuRI GGCC 2 cut(s) 164, 428
BtgI CCRYGG 3 cut(s) 10, 159, 399
BtsCI GGATG 3 cut(s) 44, 67, 135
BtsIMutI CAGTG 2 cut(s) 471, 561
BveI ACCTGC 2 cut(s) 302, 334
Cac8I GCNNGC 2 cut(s) 7, 480
CaiI CAGNNNCTG 1 cut(s) 120
Cfr13I GGNCC 2 cut(s) 163, 373
Cfr42I CCGCGG 1 cut(s) 162
Csp6I GTAC 2 cut(s) 199, 323
CviAII CATG 4 cut(s) 11, 74, 400, 479
CviQI GTAC 2 cut(s) 199, 323
DpnI GATC 4 cut(s) 221, 266, 356, 504
DpnII GATC 4 cut(s) 219, 264, 354, 502
EaeI YGGCCR 1 cut(s) 426
Eam1104I CTCTTC 2 cut(s) 233, 510
EarI CTCTTC 2 cut(s) 233, 510
Ecl136II GAGCTC 1 cut(s) 409
Eco130I CCWWGG 2 cut(s) 10, 399
Eco24I GRGCYC 1 cut(s) 411
Eco47I GGWCC 1 cut(s) 373
Eco53kI GAGCTC 1 cut(s) 409
Eco72I CACGTG 1 cut(s) 422
EcoICRI GAGCTC 1 cut(s) 409
EcoRI GAATTC 1 cut(s) 110
EcoRII CCWGG 1 cut(s) 375
EcoT14I CCWWGG 2 cut(s) 10, 399
EcoT38I GRGCYC 1 cut(s) 411
ErhI CCWWGG 2 cut(s) 10, 399
FaeI CATG 4 cut(s) 14, 77, 403, 482
FatI CATG 4 cut(s) 10, 73, 399, 478
FauNDI CATATG 1 cut(s) 215
Fnu4HI GCNGC 5 cut(s) 156, 159, 162, 426, 531
FokI GGATG 3 cut(s) 31, 54, 122
FriOI GRGCYC 1 cut(s) 411
Fsp4HI GCNGC 5 cut(s) 156, 159, 162, 426, 531
FspBI CTAG 1 cut(s) 6
GluI GCNGC 5 cut(s) 156, 159, 162, 426, 531
GsuI CTGGAG 1 cut(s) 447
HaeIII GGCC 2 cut(s) 164, 428
Hin1II CATG 4 cut(s) 14, 77, 403, 482
HinfI GANTC 6 cut(s) 86, 105, 168, 332, 460, 581
HphI GGTGA 2 cut(s) 322, 548
Hpy188I TCNGA 3 cut(s) 176, 291, 580
Hpy188III TCNNGA 1 cut(s) 271
HpyAV CCTTC 2 cut(s) 39, 139
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4IV ACGT 1 cut(s) 421
HpyCH4V TGCA 4 cut(s) 22, 346, 478, 482
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 284, 530
HpySE526I ACGT 1 cut(s) 421
Hsp92II CATG 4 cut(s) 14, 77, 403, 482
KspI CCGCGG 1 cut(s) 162
Kzo9I GATC 4 cut(s) 219, 264, 354, 502
Lsp1109I GCAGC 2 cut(s) 167, 517
MaeI CTAG 1 cut(s) 6
MaeII ACGT 1 cut(s) 421
MaeIII GTNAC 3 cut(s) 185, 415, 442
MalI GATC 4 cut(s) 221, 266, 356, 504
MboI GATC 4 cut(s) 219, 264, 354, 502
MboII GAAGA 3 cut(s) 220, 259, 527
MhlI GDGCHC 1 cut(s) 411
MluCI AATT 3 cut(s) 110, 390, 539
MlyI GAGTC 3 cut(s) 80, 99, 454
MnlI CCTC 6 cut(s) 49, 128, 248, 309, 511, 514
MroXI GAANNNNTTC 1 cut(s) 543
MslI CAYNNNNRTG 1 cut(s) 72
MspA1I CMGCKG 1 cut(s) 161
MspR9I CCNGG 1 cut(s) 377
MvaI CCWGG 1 cut(s) 377
MvnI CGCG 1 cut(s) 161
MwoI GCNNNNNNNGC 3 cut(s) 161, 284, 530
NcoI CCATGG 2 cut(s) 10, 399
NdeI CATATG 1 cut(s) 215
NdeII GATC 4 cut(s) 219, 264, 354, 502
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 4 cut(s) 14, 77, 403, 482
NlaIV GGNNCC 2 cut(s) 16, 546
NmuCI GTSAC 2 cut(s) 415, 442
NspI RCATGY 1 cut(s) 482
PaeI GCATGC 1 cut(s) 482
PdmI GAANNNNTTC 1 cut(s) 543
PfeI GAWTC 3 cut(s) 168, 332, 581
PkrI GCNGC 5 cut(s) 157, 160, 163, 427, 532
PleI GAGTC 3 cut(s) 80, 99, 454
PmaCI CACGTG 1 cut(s) 422
PmlI CACGTG 1 cut(s) 422
PpsI GAGTC 3 cut(s) 80, 99, 454
Ppu21I YACGTR 1 cut(s) 422
Psp124BI GAGCTC 1 cut(s) 411
Psp6I CCWGG 1 cut(s) 375
PspCI CACGTG 1 cut(s) 422
PspGI CCWGG 1 cut(s) 375
PspN4I GGNNCC 2 cut(s) 16, 546
PspPI GGNCC 2 cut(s) 163, 373
PstNI CAGNNNCTG 1 cut(s) 120
RsaI GTAC 2 cut(s) 200, 324
RsaNI GTAC 2 cut(s) 199, 323
RseI CAYNNNNRTG 1 cut(s) 72
SacI GAGCTC 1 cut(s) 411
SacII CCGCGG 1 cut(s) 162
SatI GCNGC 5 cut(s) 156, 159, 162, 426, 531
Sau3AI GATC 4 cut(s) 219, 264, 354, 502
Sau96I GGNCC 2 cut(s) 163, 373
SchI GAGTC 3 cut(s) 80, 99, 454
ScrFI CCNGG 1 cut(s) 377
SduI GDGCHC 1 cut(s) 411
SetI ASST 8 cut(s) 31, 41, 258, 296, 320, 328, 411, 424
SfcI CTRYAG 1 cut(s) 190
Sfr303I CCGCGG 1 cut(s) 162
SgrBI CCGCGG 1 cut(s) 162
SinI GGWCC 1 cut(s) 373
SmiMI CAYNNNNRTG 1 cut(s) 72
SmlI CTYRAG 1 cut(s) 181
SmoI CTYRAG 1 cut(s) 181
SphI GCATGC 1 cut(s) 482
Sse9I AATT 3 cut(s) 110, 390, 539
SsiI CCGC 4 cut(s) 159, 161, 308, 425
SspMI CTAG 1 cut(s) 6
SstI GAGCTC 1 cut(s) 411
StyD4I CCNGG 1 cut(s) 375
StyI CCWWGG 2 cut(s) 10, 399
TaaI ACNGT 1 cut(s) 19
TaiI ACGT 1 cut(s) 424
TaqI TCGA 1 cut(s) 108
TasI AATT 3 cut(s) 110, 390, 539
TatI WGTACW 1 cut(s) 198
TauI GCSGC 3 cut(s) 161, 164, 428
TfiI GAWTC 3 cut(s) 168, 332, 581
TscAI CASTG 2 cut(s) 471, 568
TseFI GTSAC 2 cut(s) 415, 442
TseI GCWGC 2 cut(s) 155, 530
Tsp45I GTSAC 2 cut(s) 415, 442
TspDTI ATGAA 3 cut(s) 90, 202, 220
TspGWI ACGGA 1 cut(s) 574
TspRI CASTG 2 cut(s) 471, 568
VpaK11BI GGWCC 1 cut(s) 373
XapI RAATTY 1 cut(s) 110
XceI RCATGY 1 cut(s) 482
XmnI GAANNNNTTC 1 cut(s) 543
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.