MD02G1230100.v1.1

transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010756
Physical Location & Seq
Reverse (-)
27588220 .. 27597333
9114 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1230100.v1.1.491

Sequence Viewer

Length: 2286 bp
ATGAGTGAATTTCTGGACCTCGAATCCCAAGACGGGGTTCGAATGCCATGGAACGTAATCCCAGGCACGAAGCAAGAGTCCACCAACAGCGTAGTCCCAGTCTCCGCCATCTACACTCCAATCAAGCCATTCCCCTCCATGCCCGTCCTCCCCTACGCTGCCCTCCGCTGCCGTACCTGCCGCTCCGTCCTCAACCCATTCTCCACCGTCGATTACGCCGCCAAGATCTGGATCTGCCCTTTCTGCTTCACCCGCAACCACTTCCCTCCCCACTACGCCTCCATCTCTGATGAGAACCTCCCGGCCGAGCTCTTCCCCCAGTACACAACCATCGAATACGACTCGTCGCTCGAGAAACCCACCACGCCTCCGGTCTTCATTTTCGTCGTCGATACCTGCATGATCGAGGAGGAGCTGTCGTTCCTCAAGTCCGCTCTGTCTCAGGCTCTGGGTCTCCTCCCGGACTACTCTCTCGTGGGGCTCATCACTTTCGGGACCTTCGTTCATGTCCACGAATTGGGTTTCTCCGGTGTCCCCAAAACTTACATTTTCAAAGGATCCAAGGACGTCAACAAGGAGCAGTTGCTTGAACAGATTAGTTTCTTTCTCAAGAAGCCGAAACCGGCTACTGGGGTCATTGCTGGGCACCGGGACGGATTTAGCACCGAGAGCATTGCCAGATTTTTGCTGCCCGCTTCGGATTGTGAGTTTGCACTTAATTCGGTGTTGGAGGAGTTGCAGAAGGACCCTTGGCCAGTGCCCGCAGATCAGAGGGCCACCCGATGCACCAGCACTGCCCTTAGCATTGCGGCCAGCTTGTTGGGAGCTTGCGTGCCCGGTTCTGGGGCTCGGATTATGGCATTTATTGGCGGGCCTTCCACAGAAGGCATGGGTGCTATTGTGTCAAAAAACCTATCTGAACCCATTCGTTCTCACAAGGACCTAGATAAAGATTCTGCCACACACTTCCATAAAGCCGTGAAGTTCTATGAGGGACTCTCAAGGCAGCTTGTAAACCAAGGACATGTTCTTGATCTTTTTGCTTGCGCCCTTGACCAGGTGGGGATCGCAGAACTTAAAATTGCAGTTGAAAGAACTGGAGGACTAGTTGTACTTGCTGAAAGTTTTGGCCATTCGGTGTTTAAGGACTCACTCAGACGTGTTTTTAAGTTGGGTGATTATGAGCTTGGTTTATCATCAAATGGTATATTTGAGATAAACTGCTCAAAAGATGTCAAAGTTCAGGGCATTATTGGTCCTTGTGCATCTCTTGAGAAGAAAGGTCCTTTGTGCTCGGACACGGTTGTTGGTCAGGGGAGCACTAGTGCATGGAAGATGTGTGGCCTTGACAAAGCGACATCTTTATGTCTAATATTTGAAATTGTTAAGAAGGAAATCCCTGATGCTACTGTTCAACCGACTAGCAATCAGTTTTACTTCCAGTTTCTTACTTATTATCAGCATAGTAGTGGTCAAATGAGACTCCGTGTTACGACCCTATCGAGAAGATGGGTTGCTGGACCTGGAAGCCTTCAGGAGTTGATTGCTGGATTTGACCAAGAAGCAGCTGCTGTAGTCATGGCACGCCTAGTTTCTTTCAAAATGGAAACTGAGGCAGAGTTTGACCCTATAAGATGGTTGGATAAATCATTGATACACATGTGTTCTCGGTTTGGAGATTACCAAAAGGACAGTCCCTCTTCTTTCAGTTTATCTCCACGGTTTTCAATCTTCCCTCAATTTATGTTTCATTTACGACGTTCTCAATTTGTGCAGGTTTTTAACAACAGCCCAGATGAAACAGCATACTTCAGAATGATCCTCAATCGAGAAAATGTTACCAACTCAGTTGTGATGCTTCAGCCTTCGTTGATTTCATATTCATTTCATTCAGGCCCAGAACCAGCACTCCTTGACGTCGCTGCCATTGCAGCAGATAGGATTCTGCTTTTAGACGCCTACTTTACTGTTGTTATTTTTCATGGTTCAACCATTGCTCAATGGCGGAAAGCTGGATATCAGGATCTGCCAGAACATCAGGCATTTGCTCAATTGTTACAAGCTCCTCGTGATGATGGAGATCAAATTATCAAGGAAAGATTTCCAGTACCCCGCCTGGTGATTTGCGATCAGCATGGTTCTCAGGCCCGTTTTCTTTTGGCAAAGTTGAATCCTTCTGCGACTTATAACAACGAAGCTCCACTTCCGGGAGGGGATGTGATCTTTACCGACGATGTCAGTTTTGAGGTCTTCTTGGATCATCTCCAGAGATTAGCAGTTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

762

Amino Acids

84.38

Weight (kDa)

5.88

Isoelectric Point (pI)

45.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Sec23_Sec24 PF04810 55 - 92 1.4e-16 Sec23/Sec24 zinc finger
Sec23_trunk PF04811 122 - 389 1.7e-64 Sec23/Sec24 trunk domain
Sec23_BS PF08033 400 - 505 1.5e-23 Sec23/Sec24 beta-sandwich domain
Sec23_helical PF04815 519 - 617 7.9e-26 Sec23/Sec24 helical domain
Gelsolin PF00626 632 - 719 8.3e-14 Gelsolin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2187
AatII GACGTC 2 cut(s) 570, 1920
Acc36I ACCTGC 3 cut(s) 185, 404, 1765
AccB1I GGYRCC 1 cut(s) 645
AccB7I CCANNNNNTGG 2 cut(s) 228, 517
AccBSI CCGCTC 2 cut(s) 183, 434
AclWI GGATC 7 cut(s) 239, 552, 565, 1073, 1813, 2031, 2265
AcoI YGGCCR 4 cut(s) 303, 752, 810, 1129
AcsI RAATTY 1 cut(s) 8
AcuI CTGAAG 3 cut(s) 1517, 1795, 1844
AcyI GRCGYC 3 cut(s) 567, 1917, 1956
AfaI GTAC 4 cut(s) 175, 323, 1113, 2109
AfiI CCNNNNNNNGG 9 cut(s) 33, 34, 228, 517, 629, 842, 843, 1057, 2207
AflIII ACRYGT 3 cut(s) 1024, 1159, 1659
AhlI ACTAGT 2 cut(s) 1105, 1322
AjiI CACGTC 1 cut(s) 1160
AjnI CCWGG 4 cut(s) 61, 1056, 1522, 2115
AjuI GAANNNNNNNTTGG 4 cut(s) 21, 53, 1011, 1043
AloI GAACNNNNNNTCC 4 cut(s) 404, 436, 1893, 1925
Alw21I GWGCWC 3 cut(s) 312, 1295, 1322
Alw26I GTCTC 4 cut(s) 106, 444, 458, 1474
AlwI GGATC 7 cut(s) 239, 552, 565, 1073, 1813, 2031, 2265
AlwNI CAGNNNCTG 3 cut(s) 448, 1571, 2026
Ama87I CYCGRG 1 cut(s) 350
AoxI GGCC 9 cut(s) 303, 752, 774, 810, 872, 1129, 1342, 1894, 2145
ApeKI GCWGC 8 cut(s) 158, 168, 688, 1006, 1565, 1568, 1922, 1931
ApoI RAATTY 1 cut(s) 8
ArsI GACNNNNNNTTYG 4 cut(s) 62, 94, 1678, 1710
Asp700I GAANNNNTTC 2 cut(s) 924, 2100
AspLEI GCGC 1 cut(s) 1049
AsuC2I CCSGG 5 cut(s) 302, 461, 650, 837, 2208
AsuHPI GGTGA 3 cut(s) 241, 1187, 2131
AsuII TTCGAA 1 cut(s) 40
AvaI CYCGRG 1 cut(s) 350
AvaII GGWCC 7 cut(s) 16, 495, 745, 940, 1256, 1283, 1520
BaeGI GKGCMC 3 cut(s) 648, 762, 837
BaeI ACNNNNGTAYC 2 cut(s) 1646, 1679
BalI TGGCCA 2 cut(s) 754, 1131
BamHI GGATCC 1 cut(s) 557
BanI GGYRCC 1 cut(s) 645
BanII GRGCYC 3 cut(s) 312, 483, 850
BauI CACGAG 2 cut(s) 473, 2067
BbsI GAAGAC 2 cut(s) 367, 2242
Bbv12I GWGCWC 3 cut(s) 312, 1295, 1322
BbvI GCAGC 8 cut(s) 145, 155, 675, 1018, 1555, 1577, 1909, 1943
BccI CCATC 6 cut(s) 116, 290, 338, 1503, 1629, 2069
BceAI ACGGC 2 cut(s) 156, 962
BcgI CGANNNNNNTGC 4 cut(s) 656, 690, 702, 736
BciT130I CCWGG 4 cut(s) 63, 1058, 1524, 2117
BcnI CCSGG 5 cut(s) 302, 461, 650, 837, 2208
BcoDI GTCTC 4 cut(s) 106, 444, 458, 1474
BcuI ACTAGT 2 cut(s) 1105, 1322
BfaI CTAG 5 cut(s) 944, 1106, 1323, 1422, 1589
BfmI CTRYAG 1 cut(s) 1572
BfuAI ACCTGC 3 cut(s) 185, 404, 1765
BglII AGATCT 1 cut(s) 225
Bme1390I CCNGG 9 cut(s) 63, 302, 461, 650, 837, 1058, 1524, 2117, 2208
Bme18I GGWCC 7 cut(s) 16, 495, 745, 940, 1256, 1283, 1520
BmeT110I CYCGRG 1 cut(s) 350
BmgBI CACGTC 1 cut(s) 1160
BmiI GGNNCC 4 cut(s) 496, 559, 647, 747
BmrFI CCNGG 9 cut(s) 63, 302, 461, 650, 837, 1058, 1524, 2117, 2208
BmrI ACTGGG 3 cut(s) 92, 313, 639
BmsI GCATC 4 cut(s) 773, 1274, 1393, 1845
BmuI ACTGGG 3 cut(s) 92, 313, 639
BpiI GAAGAC 2 cut(s) 367, 2242
BplI GAGNNNNNCTC 2 cut(s) 983, 1015
BpmI CTGGAG 2 cut(s) 1119, 2249
Bpu10I CCTNAGC 1 cut(s) 800
Bpu14I TTCGAA 1 cut(s) 40
BpuEI CTTGAG 4 cut(s) 410, 593, 985, 1292
BpuMI CCSGG 5 cut(s) 302, 461, 650, 837, 2208
BsaBI GATNNNNATC 2 cut(s) 230, 2079
BsaHI GRCGYC 3 cut(s) 567, 1917, 1956
BsaI GGTCTC 1 cut(s) 458
BsaJI CCNNGG 6 cut(s) 47, 61, 561, 749, 1018, 1718
BsaWI WCCGGW 2 cut(s) 370, 527
Bsc4I CCNNNNNNNGG 9 cut(s) 33, 34, 228, 517, 629, 842, 843, 1057, 2207
Bse118I RCCGGY 1 cut(s) 622
Bse1I ACTGG 7 cut(s) 98, 319, 634, 755, 1102, 1441, 2105
Bse3DI GCAATG 5 cut(s) 636, 672, 804, 1926, 1992
Bse8I GATNNNNATC 2 cut(s) 230, 2079
BseBI CCWGG 4 cut(s) 63, 1058, 1524, 2117
BseDI CCNNGG 6 cut(s) 47, 61, 561, 749, 1018, 1718
BseGI GGATG 1 cut(s) 2221
BseJI GATNNNNATC 2 cut(s) 230, 2079
BseLI CCNNNNNNNGG 9 cut(s) 33, 34, 228, 517, 629, 842, 843, 1057, 2207
BseMI GCAATG 5 cut(s) 636, 672, 804, 1926, 1992
BseMII CTCAG 5 cut(s) 455, 1168, 1602, 1860, 2156
BseNI ACTGG 7 cut(s) 98, 319, 634, 755, 1102, 1441, 2105
BseRI GAGGAG 5 cut(s) 422, 425, 446, 746, 2055
BseSI GKGCMC 3 cut(s) 648, 762, 837
BseX3I CGGCCG 1 cut(s) 303
BseXI GCAGC 8 cut(s) 145, 155, 675, 1018, 1555, 1577, 1909, 1943
BseYI CCCAGC 1 cut(s) 641
BsgI GTGCAG 1 cut(s) 1793
Bsh1285I CGRYCG 1 cut(s) 306
BshFI GGCC 9 cut(s) 305, 754, 776, 812, 874, 1131, 1344, 1896, 2147
BshNI GGYRCC 1 cut(s) 645
BsiEI CGRYCG 1 cut(s) 306
BsiHKAI GWGCWC 3 cut(s) 312, 1295, 1322
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 8 cut(s) 302, 371, 461, 528, 623, 649, 837, 2207
BslFI GGGAC 6 cut(s) 80, 508, 518, 665, 1008, 1680
BslI CCNNNNNNNGG 9 cut(s) 33, 34, 228, 517, 629, 842, 843, 1057, 2207
BsmAI GTCTC 4 cut(s) 106, 444, 458, 1474
BsmFI GGGAC 6 cut(s) 80, 508, 518, 665, 1008, 1680
BsmI GAATGC 1 cut(s) 48
BsnI GGCC 9 cut(s) 305, 754, 776, 812, 874, 1131, 1344, 1896, 2147
Bso31I GGTCTC 1 cut(s) 458
BsoBI CYCGRG 1 cut(s) 350
Bsp119I TTCGAA 1 cut(s) 40
Bsp1286I GDGCHC 8 cut(s) 312, 483, 648, 762, 837, 850, 1295, 1322
Bsp19I CCATGG 1 cut(s) 47
BspANI GGCC 9 cut(s) 305, 754, 776, 812, 874, 1131, 1344, 1896, 2147
BspCNI CTCAG 5 cut(s) 454, 1167, 1603, 1859, 2155
BspLI GGNNCC 4 cut(s) 496, 559, 647, 747
BspMI ACCTGC 3 cut(s) 185, 404, 1765
BspPI GGATC 7 cut(s) 239, 552, 565, 1073, 1813, 2031, 2265
BspQI GCTCTTC 1 cut(s) 317
BspT104I TTCGAA 1 cut(s) 40
BspT107I GGYRCC 1 cut(s) 645
BspTNI GGTCTC 1 cut(s) 458
BsrBI CCGCTC 2 cut(s) 183, 434
BsrDI GCAATG 5 cut(s) 636, 672, 804, 1926, 1992
BsrFI RCCGGY 1 cut(s) 622
BsrI ACTGG 7 cut(s) 98, 319, 634, 755, 1102, 1441, 2105
BssAI RCCGGY 1 cut(s) 622
BssECI CCNNGG 6 cut(s) 47, 61, 561, 749, 1018, 1718
BssNI GRCGYC 3 cut(s) 567, 1917, 1956
BssSI CACGAG 2 cut(s) 473, 2067
BssT1I CCWWGG 4 cut(s) 47, 561, 749, 1018
Bst2BI CACGAG 2 cut(s) 473, 2067
Bst2UI CCWGG 4 cut(s) 63, 1058, 1524, 2117
Bst4CI ACNGT 6 cut(s) 208, 1303, 1411, 1694, 1722, 1969
Bst6I CTCTTC 2 cut(s) 317, 1705
BstACI GRCGYC 3 cut(s) 567, 1917, 1956
BstBI TTCGAA 1 cut(s) 40
BstC8I GCNNGC 8 cut(s) 693, 762, 814, 829, 833, 872, 1045, 1585
BstDEI CTNAG 6 cut(s) 441, 800, 1154, 1611, 1846, 2142
BstDSI CCRYGG 2 cut(s) 47, 1718
BstENI CCTNNNNNAGG 1 cut(s) 1055
BstF5I GGATG 1 cut(s) 2221
BstHHI GCGC 1 cut(s) 1049
BstMAI GTCTC 4 cut(s) 106, 444, 458, 1474
BstMCI CGRYCG 1 cut(s) 306
BstMWI GCNNNNNNNGC 6 cut(s) 177, 243, 252, 669, 1928, 1931
BstNI CCWGG 4 cut(s) 63, 1058, 1524, 2117
BstNSI RCATGY 2 cut(s) 1028, 1663
BstSCI CCNGG 9 cut(s) 61, 300, 459, 648, 835, 1056, 1522, 2115, 2206
BstSFI CTRYAG 1 cut(s) 1572
BstSLI GKGCMC 3 cut(s) 648, 762, 837
BstV1I GCAGC 8 cut(s) 145, 155, 675, 1018, 1555, 1577, 1909, 1943
BstV2I GAAGAC 2 cut(s) 367, 2242
BstX2I RGATCY 4 cut(s) 225, 231, 557, 2023
BstXI CCANNNNNNTGG 1 cut(s) 820
BstYI RGATCY 4 cut(s) 225, 231, 557, 2023
BstZI CGGCCG 1 cut(s) 303
BsuRI GGCC 9 cut(s) 305, 754, 776, 812, 874, 1131, 1344, 1896, 2147
BtgI CCRYGG 2 cut(s) 47, 1718
BtrI CACGTC 1 cut(s) 1160
BtsCI GGATG 1 cut(s) 2221
BtsI GCAGTG 1 cut(s) 792
BtsIMutI CAGTG 2 cut(s) 762, 792
BveI ACCTGC 3 cut(s) 185, 404, 1765
Cac8I GCNNGC 8 cut(s) 693, 762, 814, 829, 833, 872, 1045, 1585
CaiI CAGNNNCTG 3 cut(s) 448, 1571, 2026
CfoI GCGC 1 cut(s) 1049
Cfr10I RCCGGY 1 cut(s) 622
CseI GACGC 1 cut(s) 1964
CsiI ACCWGGT 1 cut(s) 1056
Csp6I GTAC 4 cut(s) 174, 322, 1112, 2108
CviQI GTAC 4 cut(s) 174, 322, 1112, 2108
DdeI CTNAG 6 cut(s) 441, 800, 1154, 1611, 1846, 2142
EaeI YGGCCR 4 cut(s) 303, 752, 810, 1129
EagI CGGCCG 1 cut(s) 303
Eam1104I CTCTTC 2 cut(s) 317, 1705
EarI CTCTTC 2 cut(s) 317, 1705
EciI GGCGGA 2 cut(s) 94, 2020
Ecl136II GAGCTC 1 cut(s) 310
EclXI CGGCCG 1 cut(s) 303
Eco130I CCWWGG 4 cut(s) 47, 561, 749, 1018
Eco24I GRGCYC 3 cut(s) 312, 483, 850
Eco31I GGTCTC 1 cut(s) 458
Eco32I GATATC 1 cut(s) 2018
Eco47I GGWCC 7 cut(s) 16, 495, 745, 940, 1256, 1283, 1520
Eco52I CGGCCG 1 cut(s) 303
Eco53kI GAGCTC 1 cut(s) 310
Eco57I CTGAAG 3 cut(s) 1517, 1795, 1844
Eco88I CYCGRG 1 cut(s) 350
EcoICRI GAGCTC 1 cut(s) 310
EcoNI CCTNNNNNAGG 1 cut(s) 1055
EcoO109I RGGNCCY 4 cut(s) 495, 745, 940, 1283
EcoRII CCWGG 4 cut(s) 61, 1056, 1522, 2115
EcoRV GATATC 1 cut(s) 2018
EcoT14I CCWWGG 4 cut(s) 47, 561, 749, 1018
EcoT38I GRGCYC 3 cut(s) 312, 483, 850
ErhI CCWWGG 4 cut(s) 47, 561, 749, 1018
FalI AAGNNNNNCTT 2 cut(s) 2187, 2219
FaqI GGGAC 6 cut(s) 80, 508, 518, 665, 1008, 1680
FauI CCCGC 5 cut(s) 260, 700, 769, 863, 2120
FokI GGATG 1 cut(s) 2228
FriOI GRGCYC 3 cut(s) 312, 483, 850
FspBI CTAG 5 cut(s) 944, 1106, 1323, 1422, 1589
GlaI GCGC 1 cut(s) 1048
GsaI CCCAGC 1 cut(s) 645
GsuI CTGGAG 2 cut(s) 1119, 2249
HaeIII GGCC 9 cut(s) 305, 754, 776, 812, 874, 1131, 1344, 1896, 2147
HapII CCGG 8 cut(s) 302, 371, 461, 528, 623, 649, 837, 2207
HgaI GACGC 1 cut(s) 1964
HhaI GCGC 1 cut(s) 1049
Hin1I GRCGYC 3 cut(s) 567, 1917, 1956
Hin6I GCGC 1 cut(s) 1047
HinP1I GCGC 1 cut(s) 1047
HincII GTYRAC 1 cut(s) 571
HindII GTYRAC 1 cut(s) 571
HinfI GANTC 9 cut(s) 23, 77, 341, 953, 996, 1148, 1482, 1942, 2170
HpaII CCGG 8 cut(s) 302, 371, 461, 528, 623, 649, 837, 2207
HphI GGTGA 3 cut(s) 241, 1187, 2131
Hpy166II GTNNAC 5 cut(s) 81, 324, 511, 571, 1015
Hpy188I TCNGA 8 cut(s) 289, 700, 771, 852, 919, 1157, 1297, 1814
Hpy8I GTNNAC 5 cut(s) 81, 324, 511, 571, 1015
Hpy99I CGWCG 7 cut(s) 212, 349, 389, 392, 1761, 1922, 2234
HpyAV CCTTC 8 cut(s) 508, 736, 878, 885, 1384, 1541, 1875, 2184
HpyCH4III ACNGT 6 cut(s) 208, 1303, 1411, 1694, 1722, 1969
HpyCH4IV ACGT 5 cut(s) 54, 567, 1159, 1759, 1917
HpyCH4V TGCA 9 cut(s) 399, 713, 739, 786, 1085, 1265, 1328, 1774, 1931
HpyF10VI GCNNNNNNNGC 6 cut(s) 177, 243, 252, 669, 1928, 1931
HpyF3I CTNAG 6 cut(s) 441, 800, 1154, 1611, 1846, 2142
HpySE526I ACGT 5 cut(s) 54, 567, 1159, 1759, 1917
Hsp92I GRCGYC 3 cut(s) 567, 1917, 1956
HspAI GCGC 1 cut(s) 1047
LguI GCTCTTC 1 cut(s) 317
LmnI GCTCC 7 cut(s) 188, 412, 577, 824, 1317, 2068, 2203
Lsp1109I GCAGC 8 cut(s) 145, 155, 675, 1018, 1555, 1577, 1909, 1943
LweI GCATC 4 cut(s) 773, 1274, 1393, 1845
MabI ACCWGGT 1 cut(s) 1056
MaeI CTAG 5 cut(s) 944, 1106, 1323, 1422, 1589
MaeII ACGT 5 cut(s) 54, 567, 1159, 1759, 1917
MaeIII GTNAC 3 cut(s) 1489, 1837, 2055
MbiI CCGCTC 2 cut(s) 183, 434
MboII GAAGA 8 cut(s) 304, 367, 1288, 1345, 1518, 1692, 1723, 2242
MfeI CAATTG 1 cut(s) 2051
MflI RGATCY 4 cut(s) 225, 231, 557, 2023
MhlI GDGCHC 8 cut(s) 312, 483, 648, 762, 837, 850, 1295, 1322
MlsI TGGCCA 2 cut(s) 754, 1131
MluCI AATT 9 cut(s) 8, 515, 718, 1080, 1380, 1739, 1766, 2051, 2085
MluNI TGGCCA 2 cut(s) 754, 1131
MlyI GAGTC 5 cut(s) 86, 335, 990, 1142, 1476
MmeI TCCRAC 2 cut(s) 708, 1620
Mox20I TGGCCA 2 cut(s) 754, 1131
MroXI GAANNNNTTC 2 cut(s) 924, 2100
MscI TGGCCA 2 cut(s) 754, 1131
MseI TTAA 6 cut(s) 717, 1077, 1143, 1167, 1386, 1782
MslI CAYNNNNRTG 2 cut(s) 1363, 1467
Msp20I TGGCCA 2 cut(s) 754, 1131
MspA1I CMGCKG 2 cut(s) 168, 1568
MspI CCGG 8 cut(s) 302, 371, 461, 528, 623, 649, 837, 2207
MspR9I CCNGG 9 cut(s) 63, 302, 461, 650, 837, 1058, 1524, 2117, 2208
MunI CAATTG 1 cut(s) 2051
Mva1269I GAATGC 1 cut(s) 48
MvaI CCWGG 4 cut(s) 63, 1058, 1524, 2117
MwoI GCNNNNNNNGC 6 cut(s) 177, 243, 252, 669, 1928, 1931
NciI CCSGG 5 cut(s) 302, 461, 650, 837, 2208
NcoI CCATGG 1 cut(s) 47
NlaIV GGNNCC 4 cut(s) 496, 559, 647, 747
NmeAIII GCCGAG 1 cut(s) 331
NspI RCATGY 2 cut(s) 1028, 1663
NspV TTCGAA 1 cut(s) 40
PaeR7I CTCGAG 1 cut(s) 350
PciI ACATGT 2 cut(s) 1024, 1659
PciSI GCTCTTC 1 cut(s) 317
PcsI WCGNNNNNNNCGW 2 cut(s) 498, 1499
PctI GAATGC 1 cut(s) 48
PdmI GAANNNNTTC 2 cut(s) 924, 2100
PfeI GAWTC 4 cut(s) 23, 953, 1942, 2170
PflFI GACNNNGTC 1 cut(s) 2234
PflMI CCANNNNNTGG 2 cut(s) 228, 517
PfoI TCCNGGA 2 cut(s) 459, 2206
PleI GAGTC 5 cut(s) 85, 335, 990, 1142, 1476
PpsI GAGTC 5 cut(s) 85, 335, 990, 1142, 1476
PpuMI RGGWCCY 4 cut(s) 495, 745, 940, 1283
PscI ACATGT 2 cut(s) 1024, 1659
PsiI TTATAA 1 cut(s) 2187
Psp124BI GAGCTC 1 cut(s) 312
Psp5II RGGWCCY 4 cut(s) 495, 745, 940, 1283
Psp6I CCWGG 4 cut(s) 61, 1056, 1522, 2115
PspFI CCCAGC 1 cut(s) 641
PspGI CCWGG 4 cut(s) 61, 1056, 1522, 2115
PspN4I GGNNCC 4 cut(s) 496, 559, 647, 747
PspPPI RGGWCCY 4 cut(s) 495, 745, 940, 1283
PstNI CAGNNNCTG 3 cut(s) 448, 1571, 2026
PsuI RGATCY 4 cut(s) 225, 231, 557, 2023
PsyI GACNNNGTC 1 cut(s) 2234
PvuII CAGCTG 1 cut(s) 1568
RsaI GTAC 4 cut(s) 175, 323, 1113, 2109
RsaNI GTAC 4 cut(s) 174, 322, 1112, 2108
RseI CAYNNNNRTG 2 cut(s) 1363, 1467
SacI GAGCTC 1 cut(s) 312
SapI GCTCTTC 1 cut(s) 317
SaqAI TTAA 6 cut(s) 717, 1077, 1143, 1167, 1386, 1782
SchI GAGTC 5 cut(s) 86, 335, 990, 1142, 1476
ScrFI CCNGG 9 cut(s) 63, 302, 461, 650, 837, 1058, 1524, 2117, 2208
SduI GDGCHC 8 cut(s) 312, 483, 648, 762, 837, 850, 1295, 1322
SexAI ACCWGGT 1 cut(s) 1056
SfaNI GCATC 4 cut(s) 773, 1274, 1393, 1845
SfcI CTRYAG 1 cut(s) 1572
Sfr274I CTCGAG 1 cut(s) 350
SfuI TTCGAA 1 cut(s) 40
SinI GGWCC 7 cut(s) 16, 495, 745, 940, 1256, 1283, 1520
SlaI CTCGAG 1 cut(s) 350
SmiMI CAYNNNNRTG 2 cut(s) 1363, 1467
SmlI CTYRAG 5 cut(s) 350, 425, 608, 1000, 1271
SmoI CTYRAG 5 cut(s) 350, 425, 608, 1000, 1271
SpeI ACTAGT 2 cut(s) 1105, 1322
Sse9I AATT 9 cut(s) 8, 515, 718, 1080, 1380, 1739, 1766, 2051, 2085
SspI AATATT 1 cut(s) 1374
SspMI CTAG 5 cut(s) 944, 1106, 1323, 1422, 1589
SstI GAGCTC 1 cut(s) 312
StyD4I CCNGG 9 cut(s) 61, 300, 459, 648, 835, 1056, 1522, 2115, 2206
StyI CCWWGG 4 cut(s) 47, 561, 749, 1018
TaaI ACNGT 6 cut(s) 208, 1303, 1411, 1694, 1722, 1969
TaiI ACGT 5 cut(s) 57, 570, 1162, 1762, 1920
TaqI TCGA 9 cut(s) 21, 40, 210, 333, 351, 390, 405, 1502, 1828
TasI AATT 9 cut(s) 8, 515, 718, 1080, 1380, 1739, 1766, 2051, 2085
TatI WGTACW 2 cut(s) 321, 1111
TauI GCSGC 3 cut(s) 183, 221, 812
TfiI GAWTC 4 cut(s) 23, 953, 1942, 2170
Tru1I TTAA 6 cut(s) 717, 1077, 1143, 1167, 1386, 1782
Tru9I TTAA 6 cut(s) 717, 1077, 1143, 1167, 1386, 1782
TscAI CASTG 2 cut(s) 762, 799
TseI GCWGC 8 cut(s) 158, 168, 688, 1006, 1565, 1568, 1922, 1931
TspDTI ATGAA 8 cut(s) 367, 494, 1739, 1812, 1866, 1872, 1877, 1970
TspGWI ACGGA 3 cut(s) 175, 669, 1475
TspRI CASTG 2 cut(s) 762, 799
Tth111I GACNNNGTC 1 cut(s) 2234
Van91I CCANNNNNTGG 2 cut(s) 228, 517
VpaK11BI GGWCC 7 cut(s) 16, 495, 745, 940, 1256, 1283, 1520
XagI CCTNNNNNAGG 1 cut(s) 1055
XapI RAATTY 1 cut(s) 8
XceI RCATGY 2 cut(s) 1028, 1663
XcmI CCANNNNNNNNNTGG 1 cut(s) 886
XhoI CTCGAG 1 cut(s) 350
XmnI GAANNNNTTC 2 cut(s) 924, 2100
XspI CTAG 5 cut(s) 944, 1106, 1323, 1422, 1589
ZraI GACGTC 2 cut(s) 568, 1918
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.