MD07G1082300.v1.1

transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010756
Physical Location & Seq
Forward (+)
8058042 .. 8075809
17768 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1082300.v1.1.491

Sequence Viewer

Length: 2286 bp
ATGAGTGAATTTATGGACCTGGAATCCCAAGACGGGGTTCGAATGCCATGGAACGTAATCCCAGGCACGAAGCAAGAGTCCACCAACAGCGTAGTCCCAGTCTCCGCCATCTACACTCCGATCAAGCCATTCCCCTCCATGCCCGTCCTCCCCTACGCCCCCCTCCGCTGCCGCACCTGCCGCTCCGTCCTTAACCCATTCTCCACCGTCGATTACGCCGCCAAGATCTGGATCTGCCCTTTCTGCTTCACCCGCAACCACTTCCCTCCCCACTACGCCTCCATCTCCGACGAGAACCTCCCTGCCGAGCTCTTCCCCCAGTACACCACCATCGAGTACGACTCCTCCATCGAAAAACCCACCTCGCCTCCGGTCTTTATTTTCGTCGTCGACACCTGCATGATCGAGGAGGAGCTCTCGTTTCTCAAGTCCGCTCTGTCTCAGGCTCTCGGTCTCCTCCCGGACCACTCTCTCGTGGGGCTCATCACTTTCGGGACCTTCGTCCATGTCCACGAATTGGGTTTCCCCAATGTCCCCAAAACTTACATTTTCAAAGGATCCAAGGACGTCAACAAGGAGCAATTGCTTGAACAGATGAGTTTCTTTCTCAAGAAGCCGAAGCCGGCTACTGGGGTTATTGCGGGCCCCCGTGACGGGTTGAGCACCGAGAGCATTGCCAGATTTTTGTTGCCCGCATCAGATTGTGAGTTTGCGCTTAATTCAGTGTTGGAGGAGTTGCAGAAGGACCCTTGGCCGGTGCCCGCGGATCAGAGGGCCACCCGGTGCACCAGCACCGCGCTTAGCGTTGCTGCCAGCTTGTTGGGAGCTTGCGTGCCAGGTTCTGGAGCTCGGATCATGGCATTCATTGGTGGGCCGTCTACAGAAGGCCAGGGTGCTATTGTATCGAAAAACTTATCTGAGCCTATTCGTTCTCACAAGGACCTGGATAAGGATTCTGCTCCACACTTCCATAAAGCCGTGAAGTTCTATGAAGGACTATCAAAGCAGCTTGTTAACCAAGGACATGTTCTTGATCTTTTTGCTTGTGCCCTTGACCAGGTGGGGATCGCAGAACTTAAAATTGCTGTTGAGAGAACTGGAGGACTAGTTGTGCTTGCTGAAAGTTTTGGCCATTCGGTGTTTAAGGACTCACTCAGACGCGTTTTTCAGTCGGGTGATTATGAGCTTGGTTTGTCATCAAATGGTATATTTGAGATAAACTTCTCAAAAGATGTCAAAGTTCAGGGCATTATTGGTCCTTGTGCATCGCTTGAGAAGAAAGGTCCTCTGTGCTCGGACGCGGTTGTTGGTCAGGGGAGCACTAGTGCATGGAAGATGTGTGGCCTTGACAAAGCGACATCTTTATGTCTAATATTTGAAATTGTTAAGAAGGAAATCCCAGATGCTACTGTTCAACCCACTAGCAATCAATTTTACTTCCAGTTTCTCACTTATTATCAGCATAGTAGTGGTCAAATGAGACTTCGTGTTACAACCCTATCGAGAAGATGGGTTGCTGGACCTGGAAGCCTTCAGGAGTTGATTGCTGGGTTTGACCAAGAAGCAGCTGCTGTAGTCATGGCACGCCTAGTTTCCTTCAAAATGGAAAGTGAGGCAGAGTTTGACCCTATAAGATGGTTGGATAAATCACTGATACACATGTGTTCTCGGTTTGGAGATTACCAAAAGGACAGTCCCTCTTCTTTCAGCTTATCTCCACGGTTTTCAATCTTCCCTCAATTTATGTTTCATTTACGACGTTCTCAATTTGTCCAGGTTTTTAACAACAGCCCAGATGAAACAGCGTACTTCAGAATGATCCTAAACCGAGAAAATGTTACCAATTCAGTTGTGATGATTCAGCCTTCGTTGATTTCATATTCATTTCATTCAGGCCCAGAACCAGCACTTCTTGATGTCGCTGCCATCGCAGCAGACAGGATTCTGCTTTTAGACGCCTACTTTACTGTTGTTATTTTTCATGGTTCAACTATTGCACAATGGCGGAAAGCAGGGTATCAGGACCTGCCAGAACATCAGGCATTTGCTCAATTGTTACAAGCTCCTCGTGACGATGGAGATCAAATTAGCAAGGAAAGATTTCCAGTACCCCGCCTGGTGATTTGCGATCAGCATGGTTCTCAGGCCCGTTTTCTTTTGGCAAAGTTGAATCCTTCTGCTACCTACAACAACGAAGCTCCACTTCCGGGAGGGGATGTGATCTTTACTGACGATGTCAGTTTTGAGGTCTTCTTGGATCATCTCCAGAGATTAGCAGTTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
Gelsolin Sec23_BS Sec23_helical Sec23_trunk zf-Sec23_Sec24

Protein Analysis

762

Amino Acids

84.3

Weight (kDa)

5.81

Isoelectric Point (pI)

48.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-Sec23_Sec24 PF04810 54 - 92 3.3e-17 Sec23/Sec24 zinc finger
Sec23_trunk PF04811 122 - 389 5.3e-65 Sec23/Sec24 trunk domain
Sec23_BS PF08033 400 - 505 3.7e-22 Sec23/Sec24 beta-sandwich domain
Sec23_helical PF04815 519 - 617 7.1e-26 Sec23/Sec24 helical domain
Gelsolin PF00626 632 - 719 3.9e-14 Gelsolin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 185, 404
AatII GACGTC 1 cut(s) 570
Acc36I ACCTGC 3 cut(s) 185, 404, 2034
AccB1I GGYRCC 1 cut(s) 757
AccB7I CCANNNNNTGG 3 cut(s) 228, 517, 842
AccBSI CCGCTC 2 cut(s) 183, 434
AccI GTMKAC 2 cut(s) 390, 878
AccII CGCG 4 cut(s) 764, 797, 1161, 1301
AclWI GGATC 8 cut(s) 239, 552, 565, 774, 860, 1073, 1813, 2265
AcoI YGGCCR 2 cut(s) 752, 1129
AcsI RAATTY 1 cut(s) 8
AcuI CTGAAG 2 cut(s) 1517, 1795
AcyI GRCGYC 2 cut(s) 567, 1956
AdeI CACNNNGTG 1 cut(s) 783
AfaI GTAC 4 cut(s) 323, 338, 1808, 2109
AflIII ACRYGT 3 cut(s) 1024, 1159, 1659
AgsI TTSAA 9 cut(s) 553, 590, 1379, 1415, 1600, 1728, 1989, 2170, 2282
AhlI ACTAGT 2 cut(s) 1105, 1322
AjnI CCWGG 9 cut(s) 18, 61, 835, 888, 942, 1056, 1522, 1773, 2115
AjuI GAANNNNNNNTTGG 4 cut(s) 21, 53, 1011, 1043
Alw21I GWGCWC 7 cut(s) 312, 417, 665, 788, 850, 1295, 1322
Alw26I GTCTC 4 cut(s) 106, 444, 458, 1474
Alw44I GTGCAC 1 cut(s) 784
AlwI GGATC 8 cut(s) 239, 552, 565, 774, 860, 1073, 1813, 2265
AlwNI CAGNNNCTG 3 cut(s) 842, 1571, 2026
AoxI GGCC 9 cut(s) 643, 752, 774, 872, 886, 1129, 1342, 1894, 2145
ApaI GGGCCC 1 cut(s) 647
ApaLI GTGCAC 1 cut(s) 784
ApeKI GCWGC 7 cut(s) 168, 809, 1006, 1565, 1568, 1922, 1931
ApoI RAATTY 1 cut(s) 8
ArsI GACNNNNNNTTYG 4 cut(s) 62, 94, 1678, 1710
Asp700I GAANNNNTTC 1 cut(s) 2100
AspLEI GCGC 2 cut(s) 715, 799
AsuC2I CCSGG 3 cut(s) 461, 781, 2208
AsuHPI GGTGA 3 cut(s) 241, 1187, 2131
AsuII TTCGAA 1 cut(s) 40
AvaII GGWCC 9 cut(s) 16, 463, 495, 745, 940, 1256, 1283, 1520, 2023
BaeGI GKGCMC 4 cut(s) 647, 762, 788, 1051
BaeI ACNNNNGTAYC 2 cut(s) 1646, 1679
BalI TGGCCA 1 cut(s) 1131
BamHI GGATCC 1 cut(s) 557
BanI GGYRCC 1 cut(s) 757
BanII GRGCYC 5 cut(s) 312, 417, 483, 647, 850
BauI CACGAG 2 cut(s) 473, 2067
BbsI GAAGAC 1 cut(s) 2242
Bbv12I GWGCWC 7 cut(s) 312, 417, 665, 788, 850, 1295, 1322
BbvI GCAGC 7 cut(s) 155, 796, 1018, 1555, 1577, 1909, 1943
BccI CCATC 8 cut(s) 116, 290, 338, 356, 1503, 1629, 1934, 2069
BceAI ACGGC 2 cut(s) 859, 962
BcgI CGANNNNNNTGC 2 cut(s) 656, 690
BciT130I CCWGG 9 cut(s) 20, 63, 837, 890, 944, 1058, 1524, 1775, 2117
BcnI CCSGG 3 cut(s) 461, 781, 2208
BcoDI GTCTC 4 cut(s) 106, 444, 458, 1474
BcuI ACTAGT 2 cut(s) 1105, 1322
BfaI CTAG 4 cut(s) 1106, 1323, 1422, 1589
BfmI CTRYAG 2 cut(s) 879, 1572
BfuAI ACCTGC 3 cut(s) 185, 404, 2034
BglII AGATCT 1 cut(s) 225
BlpI GCTNAGC 1 cut(s) 800
Bme18I GGWCC 9 cut(s) 16, 463, 495, 745, 940, 1256, 1283, 1520, 2023
BmiI GGNNCC 6 cut(s) 496, 559, 645, 646, 747, 759
BmrI ACTGGG 3 cut(s) 92, 313, 639
BmsI GCATC 3 cut(s) 704, 1274, 1393
BmuI ACTGGG 3 cut(s) 92, 313, 639
BoxI GACNNNNGTC 1 cut(s) 500
BpiI GAAGAC 1 cut(s) 2242
BplI GAGNNNNNCTC 4 cut(s) 326, 358, 401, 433
BpmI CTGGAG 3 cut(s) 864, 1119, 2249
Bpu1102I GCTNAGC 1 cut(s) 800
Bpu14I TTCGAA 1 cut(s) 40
BpuEI CTTGAG 3 cut(s) 410, 593, 1292
BpuMI CCSGG 3 cut(s) 461, 781, 2208
BsaBI GATNNNNATC 2 cut(s) 230, 2079
BsaHI GRCGYC 2 cut(s) 567, 1956
BsaI GGTCTC 1 cut(s) 458
BsaJI CCNNGG 8 cut(s) 47, 61, 561, 749, 762, 889, 1018, 1718
BsaWI WCCGGW 1 cut(s) 370
Bse118I RCCGGY 2 cut(s) 622, 754
Bse1I ACTGG 6 cut(s) 98, 319, 634, 1102, 1441, 2105
Bse3DI GCAATG 1 cut(s) 672
Bse8I GATNNNNATC 2 cut(s) 230, 2079
BseBI CCWGG 9 cut(s) 20, 63, 837, 890, 944, 1058, 1524, 1775, 2117
BseDI CCNNGG 8 cut(s) 47, 61, 561, 749, 762, 889, 1018, 1718
BseGI GGATG 1 cut(s) 2221
BseJI GATNNNNATC 2 cut(s) 230, 2079
BseMI GCAATG 1 cut(s) 672
BseMII CTCAG 4 cut(s) 455, 909, 1168, 2156
BseNI ACTGG 6 cut(s) 98, 319, 634, 1102, 1441, 2105
BseRI GAGGAG 6 cut(s) 334, 422, 425, 446, 746, 2055
BseSI GKGCMC 4 cut(s) 647, 762, 788, 1051
BseXI GCAGC 7 cut(s) 155, 796, 1018, 1555, 1577, 1909, 1943
BseYI CCCAGC 1 cut(s) 1547
Bsh1236I CGCG 4 cut(s) 764, 797, 1161, 1301
BshFI GGCC 9 cut(s) 645, 754, 776, 874, 888, 1131, 1344, 1896, 2147
BshNI GGYRCC 1 cut(s) 757
BsiHKAI GWGCWC 7 cut(s) 312, 417, 665, 788, 850, 1295, 1322
BsiSI CCGG 6 cut(s) 371, 461, 623, 755, 781, 2207
BslFI GGGAC 4 cut(s) 80, 508, 518, 1680
BsmAI GTCTC 4 cut(s) 106, 444, 458, 1474
BsmFI GGGAC 4 cut(s) 80, 508, 518, 1680
BsmI GAATGC 2 cut(s) 48, 860
BsnI GGCC 9 cut(s) 645, 754, 776, 874, 888, 1131, 1344, 1896, 2147
Bso31I GGTCTC 1 cut(s) 458
Bsp119I TTCGAA 1 cut(s) 40
Bsp120I GGGCCC 1 cut(s) 643
Bsp1720I GCTNAGC 1 cut(s) 800
Bsp19I CCATGG 1 cut(s) 47
BspANI GGCC 9 cut(s) 645, 754, 776, 874, 888, 1131, 1344, 1896, 2147
BspCNI CTCAG 4 cut(s) 454, 910, 1167, 2155
BspFNI CGCG 4 cut(s) 764, 797, 1161, 1301
BspLI GGNNCC 6 cut(s) 496, 559, 645, 646, 747, 759
BspMI ACCTGC 3 cut(s) 185, 404, 2034
BspPI GGATC 8 cut(s) 239, 552, 565, 774, 860, 1073, 1813, 2265
BspQI GCTCTTC 1 cut(s) 317
BspT104I TTCGAA 1 cut(s) 40
BspT107I GGYRCC 1 cut(s) 757
BspTNI GGTCTC 1 cut(s) 458
BsrBI CCGCTC 2 cut(s) 183, 434
BsrDI GCAATG 1 cut(s) 672
BsrFI RCCGGY 2 cut(s) 622, 754
BsrI ACTGG 6 cut(s) 98, 319, 634, 1102, 1441, 2105
BssAI RCCGGY 2 cut(s) 622, 754
BssECI CCNNGG 8 cut(s) 47, 61, 561, 749, 762, 889, 1018, 1718
BssNI GRCGYC 2 cut(s) 567, 1956
BssSI CACGAG 2 cut(s) 473, 2067
BssT1I CCWWGG 4 cut(s) 47, 561, 749, 1018
Bst2BI CACGAG 2 cut(s) 473, 2067
Bst2UI CCWGG 9 cut(s) 20, 63, 837, 890, 944, 1058, 1524, 1775, 2117
Bst4CI ACNGT 5 cut(s) 208, 1411, 1694, 1722, 1969
Bst6I CTCTTC 2 cut(s) 317, 1705
BstACI GRCGYC 2 cut(s) 567, 1956
BstBI TTCGAA 1 cut(s) 40
BstC8I GCNNGC 9 cut(s) 624, 643, 693, 762, 814, 829, 833, 1116, 1585
BstDEI CTNAG 5 cut(s) 441, 800, 918, 1154, 2142
BstDSI CCRYGG 3 cut(s) 47, 762, 1718
BstENI CCTNNNNNAGG 2 cut(s) 947, 1055
BstF5I GGATG 1 cut(s) 2221
BstFNI CGCG 4 cut(s) 764, 797, 1161, 1301
BstHHI GCGC 2 cut(s) 715, 799
BstMAI GTCTC 4 cut(s) 106, 444, 458, 1474
BstMWI GCNNNNNNNGC 7 cut(s) 177, 180, 243, 252, 669, 1928, 1931
BstNI CCWGG 9 cut(s) 20, 63, 837, 890, 944, 1058, 1524, 1775, 2117
BstNSI RCATGY 2 cut(s) 1028, 1663
BstPAI GACNNNNGTC 1 cut(s) 500
BstSFI CTRYAG 2 cut(s) 879, 1572
BstSLI GKGCMC 4 cut(s) 647, 762, 788, 1051
BstUI CGCG 4 cut(s) 764, 797, 1161, 1301
BstV1I GCAGC 7 cut(s) 155, 796, 1018, 1555, 1577, 1909, 1943
BstV2I GAAGAC 1 cut(s) 2242
BstX2I RGATCY 3 cut(s) 225, 231, 557
BstXI CCANNNNNNTGG 1 cut(s) 820
BstYI RGATCY 3 cut(s) 225, 231, 557
BsuRI GGCC 9 cut(s) 645, 754, 776, 874, 888, 1131, 1344, 1896, 2147
BtgI CCRYGG 3 cut(s) 47, 762, 1718
BtgZI GCGATG 2 cut(s) 1251, 1912
BtsCI GGATG 1 cut(s) 2221
BtsIMutI CAGTG 2 cut(s) 729, 1649
BveI ACCTGC 3 cut(s) 185, 404, 2034
Cac8I GCNNGC 9 cut(s) 624, 643, 693, 762, 814, 829, 833, 1116, 1585
CaiI CAGNNNCTG 3 cut(s) 842, 1571, 2026
CfoI GCGC 2 cut(s) 715, 799
Cfr10I RCCGGY 2 cut(s) 622, 754
Cfr42I CCGCGG 1 cut(s) 765
CseI GACGC 3 cut(s) 1167, 1307, 1964
CsiI ACCWGGT 1 cut(s) 1056
Csp6I GTAC 4 cut(s) 322, 337, 1807, 2108
CviQI GTAC 4 cut(s) 322, 337, 1807, 2108
DdeI CTNAG 5 cut(s) 441, 800, 918, 1154, 2142
DraIII CACNNNGTG 1 cut(s) 783
EaeI YGGCCR 2 cut(s) 752, 1129
Eam1104I CTCTTC 2 cut(s) 317, 1705
EarI CTCTTC 2 cut(s) 317, 1705
EciI GGCGGA 2 cut(s) 94, 2020
Ecl136II GAGCTC 3 cut(s) 310, 415, 848
Eco130I CCWWGG 4 cut(s) 47, 561, 749, 1018
Eco24I GRGCYC 5 cut(s) 312, 417, 483, 647, 850
Eco31I GGTCTC 1 cut(s) 458
Eco47I GGWCC 9 cut(s) 16, 463, 495, 745, 940, 1256, 1283, 1520, 2023
Eco53kI GAGCTC 3 cut(s) 310, 415, 848
Eco57I CTGAAG 2 cut(s) 1517, 1795
EcoICRI GAGCTC 3 cut(s) 310, 415, 848
EcoNI CCTNNNNNAGG 2 cut(s) 947, 1055
EcoO109I RGGNCCY 6 cut(s) 495, 644, 745, 940, 1283, 2023
EcoRII CCWGG 9 cut(s) 18, 61, 835, 888, 942, 1056, 1522, 1773, 2115
EcoT14I CCWWGG 4 cut(s) 47, 561, 749, 1018
EcoT38I GRGCYC 5 cut(s) 312, 417, 483, 647, 850
ErhI CCWWGG 4 cut(s) 47, 561, 749, 1018
FalI AAGNNNNNCTT 2 cut(s) 2187, 2219
FaqI GGGAC 4 cut(s) 80, 508, 518, 1680
FauI CCCGC 5 cut(s) 260, 634, 700, 769, 2120
FblI GTMKAC 2 cut(s) 390, 878
FokI GGATG 1 cut(s) 2228
FriOI GRGCYC 5 cut(s) 312, 417, 483, 647, 850
FspBI CTAG 4 cut(s) 1106, 1323, 1422, 1589
GlaI GCGC 2 cut(s) 714, 798
GsaI CCCAGC 1 cut(s) 1551
GsuI CTGGAG 3 cut(s) 864, 1119, 2249
HaeIII GGCC 9 cut(s) 645, 754, 776, 874, 888, 1131, 1344, 1896, 2147
HapII CCGG 6 cut(s) 371, 461, 623, 755, 781, 2207
HgaI GACGC 3 cut(s) 1167, 1307, 1964
HhaI GCGC 2 cut(s) 715, 799
Hin1I GRCGYC 2 cut(s) 567, 1956
Hin6I GCGC 2 cut(s) 713, 797
HinP1I GCGC 2 cut(s) 713, 797
HincII GTYRAC 3 cut(s) 391, 571, 1015
HindII GTYRAC 3 cut(s) 391, 571, 1015
HinfI GANTC 8 cut(s) 23, 77, 341, 953, 1148, 1858, 1942, 2170
HpaI GTTAAC 1 cut(s) 1015
HpaII CCGG 6 cut(s) 371, 461, 623, 755, 781, 2207
HphI GGTGA 3 cut(s) 241, 1187, 2131
Hpy166II GTNNAC 8 cut(s) 81, 324, 391, 511, 571, 786, 879, 1015
Hpy188I TCNGA 9 cut(s) 120, 289, 700, 771, 852, 919, 1157, 1297, 1814
Hpy8I GTNNAC 8 cut(s) 81, 324, 391, 511, 571, 786, 879, 1015
Hpy99I CGWCG 5 cut(s) 212, 293, 389, 392, 1761
HpyAV CCTTC 9 cut(s) 508, 736, 878, 986, 1384, 1541, 1606, 1875, 2184
HpyCH4III ACNGT 5 cut(s) 208, 1411, 1694, 1722, 1969
HpyCH4IV ACGT 3 cut(s) 54, 567, 1759
HpyCH4V TGCA 6 cut(s) 399, 739, 786, 1265, 1328, 1997
HpyF10VI GCNNNNNNNGC 7 cut(s) 177, 180, 243, 252, 669, 1928, 1931
HpyF3I CTNAG 5 cut(s) 441, 800, 918, 1154, 2142
HpySE526I ACGT 3 cut(s) 54, 567, 1759
Hsp92I GRCGYC 2 cut(s) 567, 1956
HspAI GCGC 2 cut(s) 713, 797
KroI GCCGGC 1 cut(s) 622
KroNI GCCGGC 1 cut(s) 624
KspAI GTTAAC 1 cut(s) 1015
KspI CCGCGG 1 cut(s) 765
LguI GCTCTTC 1 cut(s) 317
LmnI GCTCC 9 cut(s) 188, 412, 577, 824, 845, 964, 1317, 2068, 2203
Lsp1109I GCAGC 7 cut(s) 155, 796, 1018, 1555, 1577, 1909, 1943
LweI GCATC 3 cut(s) 704, 1274, 1393
MabI ACCWGGT 1 cut(s) 1056
MaeI CTAG 4 cut(s) 1106, 1323, 1422, 1589
MaeII ACGT 3 cut(s) 54, 567, 1759
MaeIII GTNAC 5 cut(s) 650, 1489, 1837, 2055, 2069
MbiI CCGCTC 2 cut(s) 183, 434
MboII GAAGA 7 cut(s) 304, 1288, 1345, 1518, 1692, 1723, 2242
MfeI CAATTG 2 cut(s) 581, 2051
MflI RGATCY 3 cut(s) 225, 231, 557
MlsI TGGCCA 1 cut(s) 1131
MluI ACGCGT 1 cut(s) 1159
MluNI TGGCCA 1 cut(s) 1131
MlyI GAGTC 3 cut(s) 86, 335, 1142
MmeI TCCRAC 3 cut(s) 312, 708, 1620
Mox20I TGGCCA 1 cut(s) 1131
MroNI GCCGGC 1 cut(s) 622
MroXI GAANNNNTTC 1 cut(s) 2100
MscI TGGCCA 1 cut(s) 1131
MseI TTAA 7 cut(s) 192, 717, 1014, 1077, 1143, 1386, 1782
MslI CAYNNNNRTG 3 cut(s) 398, 1363, 1467
Msp20I TGGCCA 1 cut(s) 1131
MspA1I CMGCKG 3 cut(s) 168, 764, 1568
MspI CCGG 6 cut(s) 371, 461, 623, 755, 781, 2207
MunI CAATTG 2 cut(s) 581, 2051
Mva1269I GAATGC 2 cut(s) 48, 860
MvaI CCWGG 9 cut(s) 20, 63, 837, 890, 944, 1058, 1524, 1775, 2117
MvnI CGCG 4 cut(s) 764, 797, 1161, 1301
MwoI GCNNNNNNNGC 7 cut(s) 177, 180, 243, 252, 669, 1928, 1931
NaeI GCCGGC 1 cut(s) 624
NciI CCSGG 3 cut(s) 461, 781, 2208
NcoI CCATGG 1 cut(s) 47
NgoMIV GCCGGC 1 cut(s) 622
NlaIV GGNNCC 6 cut(s) 496, 559, 645, 646, 747, 759
NmeAIII GCCGAG 1 cut(s) 331
NmuCI GTSAC 2 cut(s) 650, 2069
NspI RCATGY 2 cut(s) 1028, 1663
NspV TTCGAA 1 cut(s) 40
PaqCI CACCTGC 2 cut(s) 185, 404
PciI ACATGT 2 cut(s) 1024, 1659
PciSI GCTCTTC 1 cut(s) 317
PcsI WCGNNNNNNNCGW 1 cut(s) 498
PctI GAATGC 2 cut(s) 48, 860
PdiI GCCGGC 1 cut(s) 624
PdmI GAANNNNTTC 1 cut(s) 2100
PfeI GAWTC 5 cut(s) 23, 953, 1858, 1942, 2170
PflFI GACNNNGTC 1 cut(s) 2234
PflMI CCANNNNNTGG 3 cut(s) 228, 517, 842
PfoI TCCNGGA 2 cut(s) 459, 2206
PleI GAGTC 3 cut(s) 85, 335, 1142
PpsI GAGTC 3 cut(s) 85, 335, 1142
PpuMI RGGWCCY 5 cut(s) 495, 745, 940, 1283, 2023
PscI ACATGT 2 cut(s) 1024, 1659
PshAI GACNNNNGTC 1 cut(s) 500
Psp124BI GAGCTC 3 cut(s) 312, 417, 850
Psp5II RGGWCCY 5 cut(s) 495, 745, 940, 1283, 2023
Psp6I CCWGG 9 cut(s) 18, 61, 835, 888, 942, 1056, 1522, 1773, 2115
PspFI CCCAGC 1 cut(s) 1547
PspGI CCWGG 9 cut(s) 18, 61, 835, 888, 942, 1056, 1522, 1773, 2115
PspN4I GGNNCC 6 cut(s) 496, 559, 645, 646, 747, 759
PspOMI GGGCCC 1 cut(s) 643
PspPPI RGGWCCY 5 cut(s) 495, 745, 940, 1283, 2023
PstNI CAGNNNCTG 3 cut(s) 842, 1571, 2026
PsuI RGATCY 3 cut(s) 225, 231, 557
PsyI GACNNNGTC 1 cut(s) 2234
PvuII CAGCTG 1 cut(s) 1568
RsaI GTAC 4 cut(s) 323, 338, 1808, 2109
RsaNI GTAC 4 cut(s) 322, 337, 1807, 2108
RseI CAYNNNNRTG 3 cut(s) 398, 1363, 1467
SacI GAGCTC 3 cut(s) 312, 417, 850
SacII CCGCGG 1 cut(s) 765
SalI GTCGAC 1 cut(s) 389
SapI GCTCTTC 1 cut(s) 317
SaqAI TTAA 7 cut(s) 192, 717, 1014, 1077, 1143, 1386, 1782
SchI GAGTC 3 cut(s) 86, 335, 1142
SexAI ACCWGGT 1 cut(s) 1056
SfaNI GCATC 3 cut(s) 704, 1274, 1393
SfcI CTRYAG 2 cut(s) 879, 1572
Sfr303I CCGCGG 1 cut(s) 765
SfuI TTCGAA 1 cut(s) 40
SgrBI CCGCGG 1 cut(s) 765
SinI GGWCC 9 cut(s) 16, 463, 495, 745, 940, 1256, 1283, 1520, 2023
SmiMI CAYNNNNRTG 3 cut(s) 398, 1363, 1467
SmlI CTYRAG 3 cut(s) 425, 608, 1271
SmoI CTYRAG 3 cut(s) 425, 608, 1271
SpeI ACTAGT 2 cut(s) 1105, 1322
SspI AATATT 1 cut(s) 1374
SspMI CTAG 4 cut(s) 1106, 1323, 1422, 1589
SstI GAGCTC 3 cut(s) 312, 417, 850
StyI CCWWGG 4 cut(s) 47, 561, 749, 1018
TaaI ACNGT 5 cut(s) 208, 1411, 1694, 1722, 1969
TaiI ACGT 3 cut(s) 57, 570, 1762
TaqI TCGA 8 cut(s) 40, 210, 333, 351, 390, 405, 905, 1502
TaqII GACCGA 1 cut(s) 440
TatI WGTACW 1 cut(s) 321
TauI GCSGC 3 cut(s) 174, 183, 221
TfiI GAWTC 5 cut(s) 23, 953, 1858, 1942, 2170
Tru1I TTAA 7 cut(s) 192, 717, 1014, 1077, 1143, 1386, 1782
Tru9I TTAA 7 cut(s) 192, 717, 1014, 1077, 1143, 1386, 1782
TscAI CASTG 2 cut(s) 729, 1656
TseFI GTSAC 2 cut(s) 650, 2069
TseI GCWGC 7 cut(s) 168, 809, 1006, 1565, 1568, 1922, 1931
Tsp45I GTSAC 2 cut(s) 650, 2069
TspDTI ATGAA 8 cut(s) 853, 1005, 1739, 1812, 1866, 1872, 1877, 1970
TspGWI ACGGA 1 cut(s) 175
TspRI CASTG 2 cut(s) 729, 1656
Tth111I GACNNNGTC 1 cut(s) 2234
Van91I CCANNNNNTGG 3 cut(s) 228, 517, 842
VneI GTGCAC 1 cut(s) 784
VpaK11BI GGWCC 9 cut(s) 16, 463, 495, 745, 940, 1256, 1283, 1520, 2023
XagI CCTNNNNNAGG 2 cut(s) 947, 1055
XapI RAATTY 1 cut(s) 8
XceI RCATGY 2 cut(s) 1028, 1663
XmiI GTMKAC 2 cut(s) 390, 878
XmnI GAANNNNTTC 1 cut(s) 2100
XspI CTAG 4 cut(s) 1106, 1323, 1422, 1589
ZraI GACGTC 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.