Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0012183

Physical Location & Seq

Genomic Coordinates Reverse (-)

31732199 .. 31738472

Gene Structure 6274 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1263000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4017 bp

ATGACCTCGAAGCGACCTCTTCTGATCCCATCTCCAAGAACCCCCAATACCCAACACCTCCCTGCTCTACCCGTCTATTCGGATATCGCAAATCCCACTTCCGGTGGCACTGGATCGTTTTCCGGCATGGATTCCAGAAACCCCGCTGCAGCTGACAATTCCATCAACGTTGAACCCCCGACCTTCAAGTCTTCCTCCCAAAGAAGCGTTTCTTCCCTTCAATCCAAGGCTTCCGGCAACAATTCTGTTCGGGAAAGCGCTGACAATTCCATCAACGTTGAACCCCCCACCTTCAAATCTTCCTCCCAAAGAAGCGTTTCTTCCTCCCGAAGAAGCGCTTCTTCCCTTCAATCCAAGGCTTCCAGCAACAATTCTGTTCGGGAAAGCGCTGACAATTCCATCAACGTTGAACCCCCCACCTTCAAATCTTCCTCCCAAAGAAGCGTTTCTTCCCTTCAATCCAAGGCTTCCGGCAACAATTCTGTTCGGGAAAGCGTTTCTTCCCTTCAATCCAAGGCTTCCGGCAACAATTCTGTTCGGGAAAGCGTTTCTTCCCTTCAATCCAAGAATTCCGGCAATAATTCTGTTCGGGAAGTGAGTTTCGGCGATCTCGAATCGAAGCCAGTGCGGTATGGATCGAGAGGAGCTGATTCGGAAGCGCTCAGCTTGTCCCAGAAGGAGCTGAACGAGGAGGATGTAAGGGTTGTTTATATAGATGATCTTGCTAAGACAAATGAGAGGTTTGAGTTTTCTGGTAATTCAATTAGGACGGCAAAGTACTCAGTTTTTACTTTTTTGCCTAGAAATTTGTTTGAACAGTTTCATAGGGTAGCGTACGTATATTTTCTTGTGATTGCTGTGCTCAATCAGCTCCCCCAGCTGGCGGTTTTTGGCCGTGGAGTTTCCATATTGCCACTGGCATTTGTGTTGCTTGTTACTGCTGTTAAGGATGGCTTCGAGGACTATAGGCGGCATCGGTCTGATAGAATTGAGAACAATCGTCTGGCATCAGTTTTGGTTGGTAATCAGTTCCAGTGGAAAAAGTGGAAGGATGTTCGAGTTGGTGAAATCATCAAAATTCAAGCTAGTGAAGCTATACCTTGTGATATGGTACTGCTTTCGACGAGTGATCCAACTGGGGTTGCTTATGTGCAGACAATCAATTTGGATGGGGAATCAAATTTGAAGACGCGGTATGCAAAACAAGAAACCCTCTCCAAGATGCCTGATAAGGAGAAGATTACAGGGTTGATTAAGTGTGAAAATCCCAACAGGAACATATACGGATTTCATGCATTCATGGAAATTGATGGGAAGAGATTGTCACTGGGACCCTCTAACATTATACTTCGAGGGTGTGAGCTTAAGAATACTCATTGGGTAATTGGAGTTGCTGTATATGCCGGTCGTGAGACCAAAGTTATGCTCAATAGCTCTGGAGCCCCATCCAAGAGGAGTCGCCTTGAGACCCGTATGAACTTTGAGATCATTTTACTCTCCGGGTTTCTTGTCGCTTTGTGTACAGTTGTCTCATTCTGTGCTGCGGTTTGGTTGAGACGCCACAATGATGAGTTGGATGACATACTTTTCTACAGGAAGAAGGACTACTCTGAAGAGAAAGTGGACGATTATAACTATTATGGATGGGGTTTGGAGATTCTTTTCACGTTCCTTATGTCTGTCATTGTGTTCCAGATCATGATCCCCATCTCATTGTACATTTCTATGGAGCTTGTCCGGGTGGGTCAGGCTTACTTCATGATCAGGGATGGTCAAATGTATGATGAGGCCTCTGATTCAAGATTTCAGTGCAGAGCTTTGAATATCAATGAAGATTTAGGACAAATTAAGTATGTATTCTCAGACAAAACTGGTACGCTGACAGAGAATAAAATGGAATTTCAATGTGCAAGCATTTGGGGAGTAGATTATAATGGCGGGAGAAGTAGTCCGGAGCAAGTGGGGTACTCTGTCGAAGTCAATGGGAAGATTTTGAGGCCAAAGATGAAGGTGAAGGCTGACCCAGATCTTCAGCAATTACTCAGAAGTGGAAAGGACACAAATGAAGGCAAACATGTGTACGAGTTCTTCCTTGCATTGGCAGCTTGTAATACCATTGTGCCTCTTGTTGTAGACACGTCTGATCCAAATGGGGGATTATTAGATTACCAAGGGGAGTCTCCAGATGAACAAGCATTGGTTTATGCTGCTGCTGCCTATGGTTTTATGCTTATTGAACGAACCTCTGGTCATATAGTTATCGATATCCAAGGGGATAGGCAAAGGTTCAACGTCTTGGGTTTGCATGAGTTTGATAGTGATCGGAAGAGAATGTCGGTTATATTGGGGTGCCCTGATAAGACCATCAAAGTCTTTGTAAAAGGTGCTGACACAACCATGTTCAGTGTAATAGACAAACGATTGAAATTGGATATAATACGAGCAACAGAAGCCCATATTCATGCTTACTCCTCTTTGGGTTTGAGAACACTTGTTGTGGGCATGAGAGAACTGAGTGCTACAGAATTCGAGCAGTGGCACTCATCTTTTGAGGAAGCAAGCACTGCTTTAATTGGTAGGGCTGCTCTACTTCGCAAGGTTGCTGGCAACATAGAAAACAATCTCATTATACTAGGTGCCTCTGGCATTGAAGATAAACTCCAACTGGGGGTGCCTGAAGCGATAGACTCTCTAAGAACAGCAGGGGTTCAGGTATGGGTTTTGACAGGGGACAAGCAAGAAACTGCCATATCAATCGGCTACTCATCAAAGCTTCTAACAAGAAGAATGACACAGATTGTAATTAACAGTAGTTCTAGGGATTCATGTCGACGGAGTTTAGAGGATGCCATACTAATGTCTAGGAAGCTCATGACGGTGTCTGCAGATACACAAACCGACAGAGGAAGCTCTGGACATGGTGGAACTCAAGTAGCTTTAATCATTGATGGTACCAGCCTTGTGTATATTCTTGACAGTGAACTTGAGGAAAAGCTCTTTGAATTAGCAAGTTACTGTACTGTGGTGCTTTGTTGCCGAGTGGCTCCATTGCAAAAAGCTGGAATTGTAGCCCTTGTGAAGAACAGGACGGCTGATATGACACTTGCCATTGGGGATGGTGCTAATGACGTTTCAATGATCCAAATGGCTGATGTGGGAGTTGGCATCAGTGGCCAAGAAGGTCGGCAAGCTGTCATGTCATCAGATTTTGCAATGGGACAGTTCAGATTCTTAGTTCCCCTTTTATTAATACATGGGCATTGGAATTACCAGCGGATGGGCTATATGATATTATACAATTTCTACAGGAATGCAGTGTTTGTTCTTATTTTGTTTTGGTACGTGCTCTTCACTTCTTTTACTTTGACAACTGCAATCACCGACTGGAGCAGCATGTTGTATTCTATAATCTACACGGCGGTACCTACAATTGTTGTTGGGATTCTTGACAAGGACTTAAGTAGAAGGACCCTTCTAGATTATCCTCAGCTATACGGAGCTGGTCAGAGACAAGAATGCTACAACTCAAAATTATTTTGGTTAACGGTTGTTGACACATTGTGGCAAAGTCTGGCTGTCTTCTTTATCCCTCTCTTTGCATATTGGGGTAGCAGCATTGATACATCAAGTATAGGGGATCTTTGGACGCTTGCAGTGGTAATTTTGGTTAATTTGCATTTGGCTATGGATGTTATCCGGTGGAATTGGATTACACATGCAGCCATTTGGGGATCTATAATTGCGACTTGGATTTGTGTCTTTGTCATCGATGCTATACCTTCACTGGTTGGATACTGGGCCATTTTTCAAGTCGTAAAGACTGCATCGTTTTGGTTATGTTTGTTGGCAATCATCATAGCCGCAATGGCTCCACGCTTTGTTGTAAAGTTTTTGTATCAGTACTATAGACCTAGTGATGTTCAGATTGCAAGAGAATTTGAGAGGTTTGGCAATCCAAGCGCGTCAAGGCCTGCACAAATAGAAATGAACGCGATCTTGGACACGCCGAGGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0008150 GO:0009987 GO:0010876 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0033036 GO:0034204 GO:0042623 GO:0043492 GO:0044464 GO:0045332 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1339

Amino Acids

148.99

Weight (kDa)

7.76

Isoelectric Point (pI)

43.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	236 - 302	6.5e-24	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	699 - 888	3.7e-14	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	1065 - 1305	8.3e-76	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0012183)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04930
fragaria_vesca	FvH4_7g05080 FvH4_7g05080
malus_domestica	MD02G1263000.v1.1 MD07G1054900.v1.1
prunus_persica	Prupe.2G061800_v2.0.a1
pyrus_communis	pycom02g22540 pycom07g04270
rosa_chinensis	RchiOBHm_Chr1g0331371
rosa_laevigata	RLG00000029738
rosa_multiflora	Rmu_sc0005956.1_g000004
rosa_roxburghii	Rroxscaffold_4G00319160
rosa_rugosa	Rorug01G0092900 Rorug01G0092900
rosa_samantha	Rh1AG117900 Rh1BG088700 Rh1CG112800
rosa_wichuraiana	Rw0G002870 Rw1G009440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1632, 1932
AasI	GACNNNNNNGTC	1 cut(s)	187
Acc65I	GGTACC	2 cut(s)	2951, 3421
AccB1I	GGYRCC	5 cut(s)	2349, 2636, 2671, 2951, 3421
AccB7I	CCANNNNNTGG	1 cut(s)	3277
AccI	GTMKAC	2 cut(s)	2133, 2830
AccII	CGCG	3 cut(s)	1192, 3962, 3992
AccIII	TCCGGA	1 cut(s)	1951
AclI	AACGTT	3 cut(s)	168, 276, 405
AclWI	GGATC	9 cut(s)	19, 121, 643, 1124, 1696, 2138, 3133, 3645, 3739
AcoI	YGGCCR	2 cut(s)	892, 3172
AcsI	RAATTY	7 cut(s)	568, 805, 1077, 1180, 1898, 2525, 3935
AcuI	CTGAAG	3 cut(s)	1632, 2015, 2697
AcyI	GRCGYC	1 cut(s)	1558
AdeI	CACNNNGTG	1 cut(s)	3561
AfeI	AGCGCT	4 cut(s)	259, 337, 388, 660
AfiI	CCNNNNNNNGG	7 cut(s)	101, 880, 883, 2098, 2153, 2668, 3277
AflII	CTTAAG	2 cut(s)	1364, 3457
AflIII	ACRYGT	2 cut(s)	2074, 2136
AjiI	CACGTC	1 cut(s)	2139
AjuI	GAANNNNNNNTTGG	2 cut(s)	3980, 4012
Alw21I	GWGCWC	2 cut(s)	864, 3348
Alw26I	GTCTC	6 cut(s)	1406, 1460, 1534, 1549, 2184, 3502
AlwI	GGATC	9 cut(s)	19, 121, 643, 1124, 1696, 2138, 3133, 3645, 3739
Aor13HI	TCCGGA	1 cut(s)	1951
Aor51HI	AGCGCT	4 cut(s)	259, 337, 388, 660
AoxI	GGCC	6 cut(s)	892, 1788, 1997, 3172, 3798, 3968
ApoI	RAATTY	7 cut(s)	568, 805, 1077, 1180, 1898, 2525, 3935
ArsI	GACNNNNNNTTYG	4 cut(s)	1147, 1179, 2233, 2265
AseI	ATTAAT	1 cut(s)	3248
Asp700I	GAANNNNTTC	5 cut(s)	208, 316, 337, 445, 819
Asp718I	GGTACC	2 cut(s)	2951, 3421
AspLEI	GCGC	5 cut(s)	260, 338, 389, 661, 3962
AspS9I	GGNCC	3 cut(s)	1331, 3468, 3798
AsuC2I	CCSGG	2 cut(s)	1501, 1739
AsuHPI	GGTGA	3 cut(s)	1076, 2023, 3370
AvaII	GGWCC	2 cut(s)	1331, 3468
BaeGI	GKGCMC	1 cut(s)	2354
BaeI	ACNNNNGTAYC	2 cut(s)	3405, 3438
BalI	TGGCCA	1 cut(s)	3174
BanI	GGYRCC	5 cut(s)	2349, 2636, 2671, 2951, 3421
BanII	GRGCYC	1 cut(s)	1444
BarI	GAAGNNNNNNTAC	6 cut(s)	1589, 1621, 2072, 2104, 3332, 3364
BbsI	GAAGAC	3 cut(s)	183, 1193, 3571
Bbv12I	GWGCWC	2 cut(s)	864, 3348
BbvCI	CCTCAGC	1 cut(s)	3486
BceAI	ACGGC	4 cut(s)	786, 879, 3105, 3432
BcgI	CGANNNNNNTGC	2 cut(s)	607, 641
BciVI	GTATCC	1 cut(s)	3785
BclI	TGATCA	1 cut(s)	1761
BcnI	CCSGG	2 cut(s)	1501, 1739
BcoDI	GTCTC	6 cut(s)	1406, 1460, 1534, 1549, 2184, 3502
BfaI	CTAG	7 cut(s)	801, 1086, 2633, 2817, 2862, 3476, 3912
BfmI	CTRYAG	7 cut(s)	147, 964, 1591, 2520, 2883, 3304, 3904
BfoI	RGCGCY	4 cut(s)	261, 339, 390, 662
BfrI	CTTAAG	2 cut(s)	1364, 3457
BfuI	GTATCC	1 cut(s)	3785
BglI	GCCNNNNNGGC	1 cut(s)	3866
BglII	AGATCT	1 cut(s)	2026
BlpI	GCTNAGC	1 cut(s)	662
BmcAI	AGTACT	2 cut(s)	779, 3902
Bme1390I	CCNGG	2 cut(s)	1501, 1739
Bme18I	GGWCC	2 cut(s)	1331, 3468
BmgBI	CACGTC	1 cut(s)	2139
BmgT120I	GGNCC	3 cut(s)	1331, 3468, 3798
BmrFI	CCNGG	2 cut(s)	1501, 1739
BmrI	ACTGGG	4 cut(s)	1146, 1337, 2675, 3805
BmsI	GCATC	7 cut(s)	982, 1016, 1212, 2836, 3174, 3760, 3833
BmuI	ACTGGG	4 cut(s)	1146, 1337, 2675, 3805
BpiI	GAAGAC	3 cut(s)	183, 1193, 3571
BpmI	CTGGAG	3 cut(s)	1458, 2166, 3406
Bpu10I	CCTNAGC	1 cut(s)	3486
Bpu1102I	GCTNAGC	1 cut(s)	662
BpuEI	CTTGAG	3 cut(s)	1484, 2913, 3005
BpuMI	CCSGG	2 cut(s)	1501, 1739
Bsa29I	ATCGAT	2 cut(s)	2262, 3768
BsaAI	YACGTR	2 cut(s)	838, 3343
BsaBI	GATNNNNATC	3 cut(s)	1700, 1706, 2319
BsaHI	GRCGYC	1 cut(s)	1558
BsaI	GGTCTC	2 cut(s)	1406, 1460
BsaJI	CCNNGG	8 cut(s)	225, 354, 462, 513, 895, 2170, 2269, 4007
BsaWI	WCCGGW	3 cut(s)	101, 1951, 3696
BsaXI	ACNNNNNCTCC	4 cut(s)	1380, 1410, 1933, 1963
Bsc4I	CCNNNNNNNGG	7 cut(s)	101, 880, 883, 2098, 2153, 2668, 3277
Bse118I	RCCGGY	1 cut(s)	1403
Bse3DI	GCAATG	3 cut(s)	3047, 3219, 3870
Bse8I	GATNNNNATC	3 cut(s)	1700, 1706, 2319
BseAI	TCCGGA	1 cut(s)	1951
BseCI	ATCGAT	2 cut(s)	2262, 3768
BseDI	CCNNGG	8 cut(s)	225, 354, 462, 513, 895, 2170, 2269, 4007
BseJI	GATNNNNATC	3 cut(s)	1700, 1706, 2319
BseLI	CCNNNNNNNGG	7 cut(s)	101, 880, 883, 2098, 2153, 2668, 3277
BseMI	GCAATG	3 cut(s)	3047, 3219, 3870
BseMII	CTCAG	6 cut(s)	676, 795, 1875, 2056, 2504, 3500
BseRI	GAGGAG	4 cut(s)	657, 704, 1468, 2461
BseSI	GKGCMC	1 cut(s)	2354
BseYI	CCCAGC	1 cut(s)	876
BsgI	GTGCAG	3 cut(s)	1172, 1831, 3957
Bsh1236I	CGCG	3 cut(s)	1192, 3962, 3992
Bsh1285I	CGRYCG	1 cut(s)	1408
BshFI	GGCC	6 cut(s)	894, 1790, 1999, 3174, 3800, 3970
BshNI	GGYRCC	5 cut(s)	2349, 2636, 2671, 2951, 3421
BshVI	ATCGAT	2 cut(s)	2262, 3768
BsiEI	CGRYCG	1 cut(s)	1408
BsiHKAI	GWGCWC	2 cut(s)	864, 3348
BsiWI	CGTACG	1 cut(s)	834
BslFI	GGGAC	4 cut(s)	655, 1344, 2744, 3231
BslI	CCNNNNNNNGG	7 cut(s)	101, 880, 883, 2098, 2153, 2668, 3277
BsmAI	GTCTC	6 cut(s)	1406, 1460, 1534, 1549, 2184, 3502
BsmBI	CGTCTC	1 cut(s)	1549
BsmFI	GGGAC	4 cut(s)	655, 1344, 2744, 3231
BsmI	GAATGC	3 cut(s)	1295, 3316, 3521
BsnI	GGCC	6 cut(s)	894, 1790, 1999, 3174, 3800, 3970
Bso31I	GGTCTC	2 cut(s)	1406, 1460
Bsp1286I	GDGCHC	4 cut(s)	864, 1444, 2354, 3348
Bsp13I	TCCGGA	1 cut(s)	1951
Bsp1407I	TGTACA	2 cut(s)	1520, 1716
Bsp1720I	GCTNAGC	1 cut(s)	662
BspANI	GGCC	6 cut(s)	894, 1790, 1999, 3174, 3800, 3970
BspCNI	CTCAG	6 cut(s)	675, 794, 1874, 2055, 2505, 3499
BspDI	ATCGAT	2 cut(s)	2262, 3768
BspEI	TCCGGA	1 cut(s)	1951
BspFNI	CGCG	3 cut(s)	1192, 3962, 3992
BspHI	TCATGA	3 cut(s)	1698, 1758, 2871
BspMAI	CTGCAG	2 cut(s)	151, 2887
BspPI	GGATC	9 cut(s)	19, 121, 643, 1124, 1696, 2138, 3133, 3645, 3739
BspQI	GCTCTTC	1 cut(s)	3353
BspT107I	GGYRCC	5 cut(s)	2349, 2636, 2671, 2951, 3421
BspTI	CTTAAG	2 cut(s)	1364, 3457
BspTNI	GGTCTC	2 cut(s)	1406, 1460
BsrDI	GCAATG	3 cut(s)	3047, 3219, 3870
BsrFI	RCCGGY	1 cut(s)	1403
BsrGI	TGTACA	2 cut(s)	1520, 1716
BssAI	RCCGGY	1 cut(s)	1403
BssECI	CCNNGG	8 cut(s)	225, 354, 462, 513, 895, 2170, 2269, 4007
BssNI	GRCGYC	1 cut(s)	1558
BssT1I	CCWWGG	6 cut(s)	225, 354, 462, 513, 2170, 2269
Bst4CI	ACNGT	9 cut(s)	819, 1525, 2810, 2878, 2978, 3017, 3022, 3222, 3547
Bst6I	CTCTTC	5 cut(s)	24, 1310, 1608, 2321, 3353
BstACI	GRCGYC	1 cut(s)	1558
BstAFI	CTTAAG	2 cut(s)	1364, 3457
BstAPI	GCANNNNNTGC	1 cut(s)	2564
BstAUI	TGTACA	2 cut(s)	1520, 1716
BstBAI	YACGTR	2 cut(s)	838, 3343
BstC8I	GCNNGC	7 cut(s)	882, 1912, 2560, 2605, 3189, 3651, 3972
BstDEI	CTNAG	9 cut(s)	662, 726, 781, 1861, 2042, 2513, 2693, 3232, 3486
BstDSI	CCRYGG	1 cut(s)	895
BstFNI	CGCG	3 cut(s)	1192, 3962, 3992
BstH2I	RGCGCY	4 cut(s)	261, 339, 390, 662
BstHHI	GCGC	5 cut(s)	260, 338, 389, 661, 3962
BstMAI	GTCTC	6 cut(s)	1406, 1460, 1534, 1549, 2184, 3502
BstMCI	CGRYCG	1 cut(s)	1408
BstNSI	RCATGY	3 cut(s)	2078, 3397, 3719
BstSCI	CCNGG	2 cut(s)	1499, 1737
BstSFI	CTRYAG	7 cut(s)	147, 964, 1591, 2520, 2883, 3304, 3904
BstSLI	GKGCMC	1 cut(s)	2354
BstSNI	TACGTA	1 cut(s)	838
BstUI	CGCG	3 cut(s)	1192, 3962, 3992
BstV2I	GAAGAC	3 cut(s)	183, 1193, 3571
BstX2I	RGATCY	3 cut(s)	2026, 3637, 3731
BstYI	RGATCY	3 cut(s)	2026, 3637, 3731
Bsu15I	ATCGAT	2 cut(s)	2262, 3768
BsuI	GTATCC	1 cut(s)	3785
BsuRI	GGCC	6 cut(s)	894, 1790, 1999, 3174, 3800, 3970
BsuTUI	ATCGAT	2 cut(s)	2262, 3768
BtgI	CCRYGG	1 cut(s)	895
BtrI	CACGTC	1 cut(s)	2139
BtsI	GCAGTG	4 cut(s)	2540, 2562, 3321, 3660
Cac8I	GCNNGC	7 cut(s)	882, 1912, 2560, 2605, 3189, 3651, 3972
CciI	TCATGA	3 cut(s)	1698, 1758, 2871
CfoI	GCGC	5 cut(s)	260, 338, 389, 661, 3962
Cfr10I	RCCGGY	1 cut(s)	1403
Cfr13I	GGNCC	3 cut(s)	1331, 3468, 3798
ClaI	ATCGAT	2 cut(s)	2262, 3768
CseI	GACGC	4 cut(s)	1198, 1566, 3655, 3951
DdeI	CTNAG	9 cut(s)	662, 726, 781, 1861, 2042, 2513, 2693, 3232, 3486
DraIII	CACNNNGTG	1 cut(s)	3561
DrdI	GACNNNNNNGTC	1 cut(s)	187
DseDI	GACNNNNNNGTC	1 cut(s)	187
EaeI	YGGCCR	2 cut(s)	892, 3172
Eam1104I	CTCTTC	5 cut(s)	24, 1310, 1608, 2321, 3353
EarI	CTCTTC	5 cut(s)	24, 1310, 1608, 2321, 3353
Eco105I	TACGTA	1 cut(s)	838
Eco130I	CCWWGG	6 cut(s)	225, 354, 462, 513, 2170, 2269
Eco147I	AGGCCT	2 cut(s)	1790, 3970
Eco24I	GRGCYC	1 cut(s)	1444
Eco31I	GGTCTC	2 cut(s)	1406, 1460
Eco32I	GATATC	2 cut(s)	85, 2266
Eco47I	GGWCC	2 cut(s)	1331, 3468
Eco47III	AGCGCT	4 cut(s)	259, 337, 388, 660
Eco57I	CTGAAG	3 cut(s)	1632, 2015, 2697
EcoO109I	RGGNCCY	2 cut(s)	1331, 3468
EcoRI	GAATTC	2 cut(s)	568, 2525
EcoRV	GATATC	2 cut(s)	85, 2266
EcoT14I	CCWWGG	6 cut(s)	225, 354, 462, 513, 2170, 2269
EcoT22I	ATGCAT	1 cut(s)	1297
EcoT38I	GRGCYC	1 cut(s)	1444
ErhI	CCWWGG	6 cut(s)	225, 354, 462, 513, 2170, 2269
Esp3I	CGTCTC	1 cut(s)	1549
FaqI	GGGAC	4 cut(s)	655, 1344, 2744, 3231
FauI	CCCGC	2 cut(s)	151, 1931
FbaI	TGATCA	1 cut(s)	1761
FblI	GTMKAC	2 cut(s)	2133, 2830
FriOI	GRGCYC	1 cut(s)	1444
FspBI	CTAG	7 cut(s)	801, 1086, 2633, 2817, 2862, 3476, 3912
GlaI	GCGC	5 cut(s)	259, 337, 388, 660, 3961
GsaI	CCCAGC	1 cut(s)	880
GsuI	CTGGAG	3 cut(s)	1458, 2166, 3406
HaeII	RGCGCY	4 cut(s)	261, 339, 390, 662
HaeIII	GGCC	6 cut(s)	894, 1790, 1999, 3174, 3800, 3970
HgaI	GACGC	4 cut(s)	1198, 1566, 3655, 3951
HhaI	GCGC	5 cut(s)	260, 338, 389, 661, 3962
Hin1I	GRCGYC	1 cut(s)	1558
Hin6I	GCGC	5 cut(s)	258, 336, 387, 659, 3960
HinP1I	GCGC	5 cut(s)	258, 336, 387, 659, 3960
HincII	GTYRAC	3 cut(s)	2831, 3543, 3553
HindII	GTYRAC	3 cut(s)	2831, 3543, 3553
HindIII	AAGCTT	1 cut(s)	2771
HpaI	GTTAAC	1 cut(s)	3543
HphI	GGTGA	3 cut(s)	1076, 2023, 3370
Hpy166II	GTNNAC	8 cut(s)	1521, 1624, 2080, 2134, 2831, 2981, 3543, 3553
Hpy8I	GTNNAC	8 cut(s)	1521, 1624, 2080, 2134, 2831, 2981, 3543, 3553
Hpy99I	CGWCG	2 cut(s)	1126, 2835
HpyCH4III	ACNGT	9 cut(s)	819, 1525, 2810, 2878, 2978, 3017, 3022, 3222, 3547
HpyCH4IV	ACGT	9 cut(s)	168, 276, 405, 837, 1667, 2138, 2292, 3129, 3342
HpyF3I	CTNAG	9 cut(s)	662, 726, 781, 1861, 2042, 2513, 2693, 3232, 3486
HpySE526I	ACGT	9 cut(s)	168, 276, 405, 837, 1667, 2138, 2292, 3129, 3342
Hsp92I	GRCGYC	1 cut(s)	1558
HspAI	GCGC	5 cut(s)	258, 336, 387, 659, 3960
KflI	GGGWCCC	1 cut(s)	1331
Kpn2I	TCCGGA	1 cut(s)	1951
KpnI	GGTACC	2 cut(s)	2955, 3425
Ksp22I	TGATCA	1 cut(s)	1761
KspAI	GTTAAC	1 cut(s)	3543
LguI	GCTCTTC	1 cut(s)	3353
LweI	GCATC	7 cut(s)	982, 1016, 1212, 2836, 3174, 3760, 3833
MaeI	CTAG	7 cut(s)	801, 1086, 2633, 2817, 2862, 3476, 3912
MaeII	ACGT	9 cut(s)	168, 276, 405, 837, 1667, 2138, 2292, 3129, 3342
MaeIII	GTNAC	3 cut(s)	934, 1323, 3011
MfeI	CAATTG	1 cut(s)	3429
MflI	RGATCY	3 cut(s)	2026, 3637, 3731
MhlI	GDGCHC	4 cut(s)	864, 1444, 2354, 3348
MlsI	TGGCCA	1 cut(s)	3174
MluNI	TGGCCA	1 cut(s)	3174
MlyI	GAGTC	3 cut(s)	1465, 2186, 2681
MmeI	TCCRAC	4 cut(s)	1157, 1554, 2686, 3769
Mox20I	TGGCCA	1 cut(s)	3174
Mph1103I	ATGCAT	1 cut(s)	1297
MroI	TCCGGA	1 cut(s)	1951
MroXI	GAANNNNTTC	5 cut(s)	208, 316, 337, 445, 819
MscI	TGGCCA	1 cut(s)	3174
MslI	CAYNNNNRTG	6 cut(s)	1566, 1724, 2396, 2460, 2855, 2876
Msp20I	TGGCCA	1 cut(s)	3174
MspA1I	CMGCKG	4 cut(s)	146, 152, 880, 3274
MspCI	CTTAAG	2 cut(s)	1364, 3457
MspR9I	CCNGG	2 cut(s)	1501, 1739
MunI	CAATTG	1 cut(s)	3429
Mva1269I	GAATGC	3 cut(s)	1295, 3316, 3521
MvnI	CGCG	3 cut(s)	1192, 3962, 3992
NciI	CCSGG	2 cut(s)	1501, 1739
NmeAIII	GCCGAG	1 cut(s)	3062
NmuCI	GTSAC	1 cut(s)	1323
NsiI	ATGCAT	1 cut(s)	1297
NspI	RCATGY	3 cut(s)	2078, 3397, 3719
PagI	TCATGA	3 cut(s)	1698, 1758, 2871
PceI	AGGCCT	2 cut(s)	1790, 3970
PciI	ACATGT	1 cut(s)	2074
PciSI	GCTCTTC	1 cut(s)	3353
PcsI	WCGNNNNNNNCGW	1 cut(s)	609
PctI	GAATGC	3 cut(s)	1295, 3316, 3521
PdmI	GAANNNNTTC	5 cut(s)	208, 316, 337, 445, 819
PfeI	GAWTC	9 cut(s)	131, 614, 650, 1175, 1657, 1796, 2822, 3228, 3442
Pfl23II	CGTACG	1 cut(s)	834
PflFI	GACNNNGTC	1 cut(s)	2878
PflMI	CCANNNNNTGG	1 cut(s)	3277
PleI	GAGTC	3 cut(s)	1464, 2185, 2681
PpsI	GAGTC	3 cut(s)	1464, 2185, 2681
Ppu21I	YACGTR	2 cut(s)	838, 3343
PpuMI	RGGWCCY	2 cut(s)	1331, 3468
PscI	ACATGT	1 cut(s)	2074
PshBI	ATTAAT	1 cut(s)	3248
PsiI	TTATAA	2 cut(s)	1632, 1932
Psp1406I	AACGTT	3 cut(s)	168, 276, 405
Psp5II	RGGWCCY	2 cut(s)	1331, 3468
PspFI	CCCAGC	1 cut(s)	876
PspLI	CGTACG	1 cut(s)	834
PspPI	GGNCC	3 cut(s)	1331, 3468, 3798
PspPPI	RGGWCCY	2 cut(s)	1331, 3468
PsrI	GAACNNNNNNTAC	2 cut(s)	31, 63
PstI	CTGCAG	2 cut(s)	151, 2887
PsuI	RGATCY	3 cut(s)	2026, 3637, 3731
PsyI	GACNNNGTC	1 cut(s)	2878
PvuII	CAGCTG	2 cut(s)	152, 880
RseI	CAYNNNNRTG	6 cut(s)	1566, 1724, 2396, 2460, 2855, 2876
SalI	GTCGAC	1 cut(s)	2829
SapI	GCTCTTC	1 cut(s)	3353
Sau96I	GGNCC	3 cut(s)	1331, 3468, 3798
ScaI	AGTACT	2 cut(s)	779, 3902
SchI	GAGTC	3 cut(s)	1465, 2186, 2681
ScrFI	CCNGG	2 cut(s)	1501, 1739
SduI	GDGCHC	4 cut(s)	864, 1444, 2354, 3348
SfaNI	GCATC	7 cut(s)	982, 1016, 1212, 2836, 3174, 3760, 3833
SfcI	CTRYAG	7 cut(s)	147, 964, 1591, 2520, 2883, 3304, 3904
SinI	GGWCC	2 cut(s)	1331, 3468
SmiMI	CAYNNNNRTG	6 cut(s)	1566, 1724, 2396, 2460, 2855, 2876
SmlI	CTYRAG	5 cut(s)	1364, 1463, 2928, 2984, 3457
SmoI	CTYRAG	5 cut(s)	1364, 1463, 2928, 2984, 3457
SnaBI	TACGTA	1 cut(s)	838
SseBI	AGGCCT	2 cut(s)	1790, 3970
SspMI	CTAG	7 cut(s)	801, 1086, 2633, 2817, 2862, 3476, 3912
StuI	AGGCCT	2 cut(s)	1790, 3970
StyD4I	CCNGG	2 cut(s)	1499, 1737
StyI	CCWWGG	6 cut(s)	225, 354, 462, 513, 2170, 2269
TaaI	ACNGT	9 cut(s)	819, 1525, 2810, 2878, 2978, 3017, 3022, 3222, 3547
TaiI	ACGT	9 cut(s)	171, 279, 408, 840, 1670, 2141, 2295, 3132, 3345
TaqII	GACCGA	1 cut(s)	966
TatI	WGTACW	5 cut(s)	777, 1520, 1716, 3017, 3900
TauI	GCSGC	2 cut(s)	973, 3863
TfiI	GAWTC	9 cut(s)	131, 614, 650, 1175, 1657, 1796, 2822, 3228, 3442
TseFI	GTSAC	1 cut(s)	1323
Tsp45I	GTSAC	1 cut(s)	1323
TspGWI	ACGGA	3 cut(s)	1299, 2848, 3510
Tth111I	GACNNNGTC	1 cut(s)	2878
Van91I	CCANNNNNTGG	1 cut(s)	3277
Vha464I	CTTAAG	2 cut(s)	1364, 3457
VpaK11BI	GGWCC	2 cut(s)	1331, 3468
VspI	ATTAAT	1 cut(s)	3248
XapI	RAATTY	7 cut(s)	568, 805, 1077, 1180, 1898, 2525, 3935
XbaI	TCTAGA	1 cut(s)	3475
XceI	RCATGY	3 cut(s)	2078, 3397, 3719
XcmI	CCANNNNNNNNNTGG	1 cut(s)	913
XmiI	GTMKAC	2 cut(s)	2133, 2830
XmnI	GAANNNNTTC	5 cut(s)	208, 316, 337, 445, 819
XspI	CTAG	7 cut(s)	801, 1086, 2633, 2817, 2862, 3476, 3912
ZrmI	AGTACT	2 cut(s)	779, 3902
Zsp2I	ATGCAT	1 cut(s)	1297

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.