RLG00000029738

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0012183
Physical Location & Seq
Reverse (-)
48103661 .. 48110402
6742 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029738

Sequence Viewer

Length: 3375 bp
ATGACCTCAAACCGGCCTCTTTTGATCCCTTCTCCAAGAACACCCAACACCCACGATCTCCCAACTCTACCTGTATTTCCCGATTCAGCAAAACCCAGTTCCGCCATGGATTCTCAAAACCCAGAACCCACTTTGAATTCTTCTTCTTCAAGACGTAGCATTTCTTCCGTTCAATCCAAGCCGTCCATGCGGTATGGTTCCAGAGGAGCTGATTCCGAAGGCGCCGGGTCGCAGAAGGAAATGAACGATGAGGATGTCAGGATCATACACATCAATGATCTTGCCCACACCAATGAGAGGTTTGAGTTTTCTGGCAATTCTATAAGGACCGCAAAGTACTCGGTTATCACTTTCTTGCCTAGGAATTTGTTTGAACAGTTTCATAGAGTAGCATATCTGTATTTTCTAGTGATTGCTGTGCTCAATCAGCTTCCCCAGCTGGCAGTTTTCGGCCGGGGAGTTTCCATTTTGCCATTGGCATTTGTGTTGTTAGTTACTGCTGTTAAGGATGCTTATGAGGACTATAGGAGGCATCGATCCGACCGGATTGAGAACAATCGCCTTGCTTCCGTTTTTGTTAACAATCAGTTTCAGTTGAAGAAATGGAAGGATGTACAAGTTGGTGAAATCATCAAAATTGAAGCAAATGAGGGTATTCCTTGTGATATGGTGCTGCTCTCCACCAGTGACCCAACTGGTGTTGCTTATGTGCAGACTATCAATTTAGATGGGGAGTCAAATTTGAAGACCCGGTATGCGAAGCAAGAAACCCTCTCCAAGTTGCCTGAAAAGGACAGGATTACTGGGATGATCAAGTGTGAGAAGCCCAACAGGAATATTTATGGATTTCAGGCATTCATGGAGATTGATGGCAAAAGACTGTCATTAGGTCCCTCCAACATTGTGCTTCGAGGCTGTGAGCTCAAGAATACTCATTGGGCCCTTGGGGTGGCTGTATATGCTGGTCGTGAGACCAAAGTTATGCTCAATAGCTCCGGAGCCCCATCCAAGAGGAGTCGTCTCGAGACCCGTATGAACTACGAGATCATCATGCTCTCGCTGTTTCTTGTTGTCTTGTGTTCAGTTGTCTCTCTGTGTGCTGCTGTTTGGTTGAGGCGCGAAGATGATAATTTGGACGACATACTTTTCTACAGGAAGAAGGACTACTCTGAAGCCGAAGTCGAGGATTATAAGTATTATGGATGGGGATTAGAGATTGTTTTCACATTCCTCATGTCTATCATTGTGTTCCAGATCATGATCCCCATCTCTTTGTACATTTCTATGGAGCTCGTCCGGGTGGGTCAGGCTTACTTCATGATACGAGATAGTCAAATGTATGATGAGGCTTCTAATGCAAGATTTCAGTGCAGAGCTTTGAATATAAATGAAGATTTAGGACAAATTAAGTATGTATTCACGGACAAAACTGGTACACTTACCGAGAACAAAATGGAATTTCATTGTGCAAGCATTTGGGGAGTTGATTATAATGGTGGGAGAGGCAGTTTGGAAAAGGACCAATTGGTGCACAATGTTCAAATCGATGGAATTGTTTTGAGGCCAAAGATGAATGTAAAGGCTGACCCAGATCTTCTACGACTACTAAGAAGTGAAAAAGACACAAATGAAGGCAAACATGTGCACGAGTTCTTCCTCGCGTTGGCAGCTTGTAATACTATTGTGCCTCTTGTTACAGACTCGTCTGATCCAAATGTGAGATTGATTGATTACCAAGGGGAGTCTCCAGATGAACAGGCATTAGTTTATGCTGCCGCCGCCTATGGTTTTATGCTTATTGAAAGAACGTCTGGTCATATAACTATTGATATCCAAGGAGAAAGGCAAAGGTTCAGTGTTCTGGGTTTGCATGAATTTGATAGTGACCGGAAGAGAATGTCGGTGATATTGGGGTGCCCTGATAAAACTGTGAAAGTGTTTGTAAAAGGTGCTGACACTACCATGTTCAGTGTAACAGACAGAAGATTGAACTTGAACATAATACGCGCAACAGAAGCCCATATTCATGCTTACTCCTCTCTGGGTTTGAGAACACTTGTTGTGGGGATGAGAGAACTCACTGCTTCAGAATTTGAGCAGTGGCACTCTTCTTTTGAGGCAGCAAGCACTGCTTTAATAGGCAGGGCTGCTCTGCTTCGCAAGGTTGCTGGCAACATAGAGAACAATCTCATAATTTTAGGTGCCTCTGGTATTGAAGACAAACTGCAACTGGGGGTGCCTGAGGCCATAGACTCTCTAAGGACTGCAGGGGTTCAGGTGTGGGTTTTGACGGGGGACAAGCAAGAAACTGCCATATCAATTGGCTACTCATCAAAGCTTCTTACAAGGAGGATGACCCAGATTTTAATTAAAAGCAGCTCTAAGGAGTCATGTAGACGGAATTTAGAAGATGCCATACTCATGTCTAAGAAGCTCACGACCGTGTCTGGCAATGAACACAGTACCCAAGGAAGTTCTGGAGATGGTATTACTTCATTAGCTTTAATTATCGATGGTACCAGCCTTGTTTATATTCTTGACAGTGAACTTGAGGAAAAGCTATTTGAATTAGCAAATAGATGTTCTGTGGTGCTCTGTTGCCGAGTGGCTCCTCTGCAAAAAGCTGGAATTGTTGCCCTTGTAAAAAACAGAACTGCTGATATGGCACTTGCCATTGGGGATGGTGCTAATGATGTTTCGATGATCCAAATGGCTGATGTTGGAGTTGGAATCAGTGGCCAAGAGGGTCGACAAGCTGTCATGGCTTCAGATTTTGCAATGGGGCAGTTCAGATTCTTAGTTCCCCTCTTATTGGTACATGGGCATTGGAATTACCAGCGAATGGGCTACATGATACTATACAACTTTTATAGGAATGCTGTGTTTGTTCTTATTTTGTTTTGGTATGTGCTCTTTACTTGTTTTACTCTGACAACTGCAATCACGGAATGGAGCAGCATGTTGTATTCTATAATCTACACTGCATTACCTACAATAGTTGTTGGGGTTCTTGACAAGGACCTAAGTAGAATGACCCTTCTATCATATCCTCAGCTATATGGAGCTGGTCACAGACAAGAGTGCTATAACACGAAATTATTTTGGGTAACAATGGTTGACACATTGTGGCAAAGTGTGGCTGTCTTCTTTATCCCCCTCTTTGCATATTGGGGTAGCACCATTGATACCTCAAGTATAGGAGATCTTTGGACTCTTTCGGTGGTTATATTGGTTAATATACACTTGGCTATGGATATTATTCGGTGGAGTTGGATTACACATGCAGCTATTTGGGGATCTATCATTGCGACTTGGATTTGTGTCATTGTAATTGATGCTATACCTTCACTGGTTGGCTACTGGTATGTCCCCTAA

Protein Analysis

1125

Amino Acids

126.08

Weight (kDa)

6.28

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PhoLip_ATPase_N PF16209 89 - 155 8.9e-25 Phospholipid-translocating ATPase N-terminal
Cation_ATPase PF13246 552 - 743 3.7e-14 P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C PF16212 920 - 1116 2.5e-65 Phospholipid-translocating P-type ATPase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012183)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04930
fragaria_vesca FvH4_7g05080 FvH4_7g05080
malus_domestica MD02G1263000.v1.1 MD07G1054900.v1.1
prunus_persica Prupe.2G061800_v2.0.a1
pyrus_communis pycom02g22540 pycom07g04270
rosa_chinensis RchiOBHm_Chr1g0331371
rosa_laevigata RLG00000029738
rosa_multiflora Rmu_sc0005956.1_g000004
rosa_roxburghii Rroxscaffold_4G00319160
rosa_rugosa Rorug01G0092900 Rorug01G0092900
rosa_samantha Rh1AG117900 Rh1BG088700 Rh1CG112800
rosa_wichuraiana Rw0G002870 Rw1G009440

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1191, 1491
Acc65I GGTACC 1 cut(s) 2516
AccB1I GGYRCC 5 cut(s) 221, 1914, 2201, 2236, 2516
AccB7I CCANNNNNTGG 1 cut(s) 2842
AccI GTMKAC 2 cut(s) 2395, 2749
AccII CGCG 3 cut(s) 1119, 1661, 2007
AccIII TCCGGA 1 cut(s) 995
AciI CCGC 5 cut(s) 102, 190, 330, 1776, 1779
AclWI GGATC 7 cut(s) 19, 269, 531, 1255, 1703, 2698, 3304
AcoI YGGCCR 2 cut(s) 451, 2737
AcsI RAATTY 7 cut(s) 136, 364, 739, 1457, 1874, 2090, 2401
AcuI CTGAAG 3 cut(s) 1191, 2070, 2751
AcyI GRCGYC 1 cut(s) 222
AdeI CACNNNGTG 1 cut(s) 3126
AfaI GTAC 7 cut(s) 338, 615, 1277, 1435, 2464, 2518, 2817
AfiI CCNNNNNNNGG 6 cut(s) 12, 297, 949, 1663, 2812, 2842
AflIII ACRYGT 1 cut(s) 1639
AhdI GACNNNNNGTC 1 cut(s) 2756
AleI CACNNNNGTG 1 cut(s) 2441
Alw21I GWGCWC 7 cut(s) 423, 924, 1293, 1533, 1647, 2595, 2913
Alw26I GTCTC 5 cut(s) 965, 1019, 1025, 1093, 1749
Alw44I GTGCAC 2 cut(s) 1529, 1643
AlwI GGATC 7 cut(s) 19, 269, 531, 1255, 1703, 2698, 3304
Ama87I CYCGRG 1 cut(s) 1022
Aor13HI TCCGGA 1 cut(s) 995
AoxI GGCC 6 cut(s) 14, 451, 939, 1562, 2244, 2737
ApaI GGGCCC 1 cut(s) 943
ApaLI GTGCAC 2 cut(s) 1529, 1643
ApeKI GCWGC 9 cut(s) 673, 1100, 1667, 1772, 2120, 2147, 2376, 2955, 3284
ApoI RAATTY 7 cut(s) 136, 364, 739, 1457, 1874, 2090, 2401
Asp700I GAANNNNTTC 1 cut(s) 378
Asp718I GGTACC 1 cut(s) 2516
AspA2I CCTAGG 1 cut(s) 359
AspLEI GCGC 3 cut(s) 224, 1119, 2009
AspS9I GGNCC 6 cut(s) 327, 890, 939, 940, 1519, 3019
AsuC2I CCSGG 4 cut(s) 226, 455, 751, 1298
AsuHPI GGTGA 2 cut(s) 635, 1915
AvaI CYCGRG 1 cut(s) 1022
AvaII GGWCC 4 cut(s) 327, 890, 1519, 3019
AvrII CCTAGG 1 cut(s) 359
AxyI CCTNAGG 1 cut(s) 2241
BaeGI GKGCMC 4 cut(s) 943, 1533, 1647, 1919
BalI TGGCCA 1 cut(s) 2739
BanI GGYRCC 5 cut(s) 221, 1914, 2201, 2236, 2516
BanII GRGCYC 4 cut(s) 924, 943, 1003, 1293
BarI GAAGNNNNNNTAC 6 cut(s) 148, 180, 1148, 1180, 2400, 2432
BauI CACGAG 1 cut(s) 1646
BbsI GAAGAC 3 cut(s) 752, 2223, 3136
Bbv12I GWGCWC 7 cut(s) 423, 924, 1293, 1533, 1647, 2595, 2913
BbvCI CCTCAGC 1 cut(s) 3051
BbvI GCAGC 9 cut(s) 660, 1087, 1679, 1759, 2132, 2134, 2388, 2967, 3296
BccI CCATC 9 cut(s) 722, 863, 1012, 1197, 1274, 1541, 2477, 2507, 2675
BceAI ACGGC 1 cut(s) 166
BcgI CGANNNNNNTGC 2 cut(s) 321, 355
BclI TGATCA 1 cut(s) 810
BcnI CCSGG 4 cut(s) 226, 455, 751, 1298
BcoDI GTCTC 5 cut(s) 965, 1019, 1025, 1093, 1749
BfaI CTAG 2 cut(s) 360, 407
BfmI CTRYAG 3 cut(s) 523, 1150, 2265
BfoI RGCGCY 1 cut(s) 225
BglII AGATCT 2 cut(s) 1591, 3202
BlnI CCTAGG 1 cut(s) 359
BmcAI AGTACT 1 cut(s) 338
Bme1390I CCNGG 4 cut(s) 226, 455, 751, 1298
Bme18I GGWCC 4 cut(s) 327, 890, 1519, 3019
BmeRI GACNNNNNGTC 1 cut(s) 2756
BmeT110I CYCGRG 1 cut(s) 1022
BmgT120I GGNCC 6 cut(s) 327, 890, 939, 940, 1519, 3019
BmrFI CCNGG 4 cut(s) 226, 455, 751, 1298
BmrI ACTGGG 3 cut(s) 90, 813, 2240
BmsI GCATC 4 cut(s) 499, 541, 2401, 3325
BmuI ACTGGG 3 cut(s) 90, 813, 2240
BpiI GAAGAC 3 cut(s) 752, 2223, 3136
BpmI CTGGAG 2 cut(s) 1731, 2499
Bpu10I CCTNAGC 1 cut(s) 3051
BpuEI CTTGAG 3 cut(s) 908, 2570, 3175
BpuMI CCSGG 4 cut(s) 226, 455, 751, 1298
Bsa29I ATCGAT 3 cut(s) 535, 1545, 2511
BsaBI GATNNNNATC 2 cut(s) 1259, 1265
BsaHI GRCGYC 1 cut(s) 222
BsaI GGTCTC 2 cut(s) 965, 1019
BsaJI CCNNGG 7 cut(s) 105, 359, 454, 943, 1735, 1834, 2467
BsaWI WCCGGW 3 cut(s) 543, 995, 1887
BsaXI ACNNNNNCTCC 2 cut(s) 1492, 1522
Bsc4I CCNNNNNNNGG 6 cut(s) 12, 297, 949, 1663, 2812, 2842
Bse118I RCCGGY 1 cut(s) 12
Bse1I ACTGG 8 cut(s) 96, 684, 700, 808, 1435, 2235, 3354, 3365
Bse21I CCTNAGG 1 cut(s) 2241
Bse3DI GCAATG 3 cut(s) 2458, 2784, 3303
Bse8I GATNNNNATC 2 cut(s) 1259, 1265
BseAI TCCGGA 1 cut(s) 995
BseCI ATCGAT 3 cut(s) 535, 1545, 2511
BseDI CCNNGG 7 cut(s) 105, 359, 454, 943, 1735, 1834, 2467
BseGI GGATG 9 cut(s) 259, 514, 616, 813, 1004, 1208, 2073, 2358, 2686
BseJI GATNNNNATC 2 cut(s) 1259, 1265
BseLI CCNNNNNNNGG 6 cut(s) 12, 297, 949, 1663, 2812, 2842
BseMI GCAATG 3 cut(s) 2458, 2784, 3303
BseMII CTCAG 2 cut(s) 2232, 3065
BseNI ACTGG 8 cut(s) 96, 684, 700, 808, 1435, 2235, 3354, 3365
BseRI GAGGAG 4 cut(s) 219, 1027, 2026, 2601
BseSI GKGCMC 4 cut(s) 943, 1533, 1647, 1919
BseX3I CGGCCG 1 cut(s) 451
BseXI GCAGC 9 cut(s) 660, 1087, 1679, 1759, 2132, 2134, 2388, 2967, 3296
BseYI CCCAGC 1 cut(s) 435
BsgI GTGCAG 2 cut(s) 731, 1390
Bsh1236I CGCG 3 cut(s) 1119, 1661, 2007
Bsh1285I CGRYCG 3 cut(s) 454, 544, 2442
BshFI GGCC 6 cut(s) 16, 453, 941, 1564, 2246, 2739
BshNI GGYRCC 5 cut(s) 221, 1914, 2201, 2236, 2516
BshVI ATCGAT 3 cut(s) 535, 1545, 2511
BsiEI CGRYCG 3 cut(s) 454, 544, 2442
BsiHKAI GWGCWC 7 cut(s) 423, 924, 1293, 1533, 1647, 2595, 2913
BsiHKCI CYCGRG 1 cut(s) 1022
BsiSI CCGG 8 cut(s) 13, 225, 454, 544, 751, 996, 1297, 1888
BslFI GGGAC 3 cut(s) 876, 2309, 3353
BslI CCNNNNNNNGG 6 cut(s) 12, 297, 949, 1663, 2812, 2842
BsmAI GTCTC 5 cut(s) 965, 1019, 1025, 1093, 1749
BsmBI CGTCTC 1 cut(s) 1025
BsmFI GGGAC 3 cut(s) 876, 2309, 3353
BsmI GAATGC 2 cut(s) 854, 2881
BsnI GGCC 6 cut(s) 16, 453, 941, 1564, 2246, 2739
Bso31I GGTCTC 2 cut(s) 965, 1019
BsoBI CYCGRG 1 cut(s) 1022
Bsp120I GGGCCC 1 cut(s) 939
Bsp13I TCCGGA 1 cut(s) 995
Bsp1407I TGTACA 2 cut(s) 613, 1275
Bsp19I CCATGG 1 cut(s) 105
BspACI CCGC 5 cut(s) 102, 190, 330, 1776, 1779
BspANI GGCC 6 cut(s) 16, 453, 941, 1564, 2246, 2739
BspCNI CTCAG 2 cut(s) 2233, 3064
BspDI ATCGAT 3 cut(s) 535, 1545, 2511
BspEI TCCGGA 1 cut(s) 995
BspFNI CGCG 3 cut(s) 1119, 1661, 2007
BspHI TCATGA 2 cut(s) 1257, 1317
BspMAI CTGCAG 1 cut(s) 2269
BspPI GGATC 7 cut(s) 19, 269, 531, 1255, 1703, 2698, 3304
BspT107I GGYRCC 5 cut(s) 221, 1914, 2201, 2236, 2516
BspTNI GGTCTC 2 cut(s) 965, 1019
BsrDI GCAATG 3 cut(s) 2458, 2784, 3303
BsrFI RCCGGY 1 cut(s) 12
BsrGI TGTACA 2 cut(s) 613, 1275
BsrI ACTGG 8 cut(s) 96, 684, 700, 808, 1435, 2235, 3354, 3365
BssAI RCCGGY 1 cut(s) 12
BssECI CCNNGG 7 cut(s) 105, 359, 454, 943, 1735, 1834, 2467
BssNI GRCGYC 1 cut(s) 222
BssSI CACGAG 1 cut(s) 1646
BssT1I CCWWGG 6 cut(s) 105, 359, 943, 1735, 1834, 2467
Bst2BI CACGAG 1 cut(s) 1646
Bst4CI ACNGT 6 cut(s) 378, 882, 1930, 2443, 2462, 2543
Bst6I CTCTTC 2 cut(s) 1886, 2113
BstACI GRCGYC 1 cut(s) 222
BstAPI GCANNNNNTGC 1 cut(s) 2129
BstAUI TGTACA 2 cut(s) 613, 1275
BstC8I GCNNGC 4 cut(s) 441, 1471, 2125, 2170
BstDEI CTNAG 8 cut(s) 1607, 2241, 2258, 2382, 2427, 2797, 3023, 3051
BstDSI CCRYGG 1 cut(s) 105
BstF5I GGATG 9 cut(s) 259, 514, 616, 813, 1004, 1208, 2073, 2358, 2686
BstFNI CGCG 3 cut(s) 1119, 1661, 2007
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 3 cut(s) 224, 1119, 2009
BstMAI GTCTC 5 cut(s) 965, 1019, 1025, 1093, 1749
BstMCI CGRYCG 3 cut(s) 454, 544, 2442
BstNSI RCATGY 3 cut(s) 1643, 2962, 3284
BstSCI CCNGG 4 cut(s) 224, 453, 749, 1296
BstSFI CTRYAG 3 cut(s) 523, 1150, 2265
BstSLI GKGCMC 4 cut(s) 943, 1533, 1647, 1919
BstUI CGCG 3 cut(s) 1119, 1661, 2007
BstV1I GCAGC 9 cut(s) 660, 1087, 1679, 1759, 2132, 2134, 2388, 2967, 3296
BstV2I GAAGAC 3 cut(s) 752, 2223, 3136
BstX2I RGATCY 3 cut(s) 1591, 3202, 3296
BstYI RGATCY 3 cut(s) 1591, 3202, 3296
BstZI CGGCCG 1 cut(s) 451
Bsu15I ATCGAT 3 cut(s) 535, 1545, 2511
Bsu36I CCTNAGG 1 cut(s) 2241
BsuRI GGCC 6 cut(s) 16, 453, 941, 1564, 2246, 2739
BsuTUI ATCGAT 3 cut(s) 535, 1545, 2511
BtgI CCRYGG 1 cut(s) 105
BtsCI GGATG 9 cut(s) 259, 514, 616, 813, 1004, 1208, 2073, 2358, 2686
BtsI GCAGTG 4 cut(s) 2079, 2105, 2127, 2979
Cac8I GCNNGC 4 cut(s) 441, 1471, 2125, 2170
CciI TCATGA 2 cut(s) 1257, 1317
CfoI GCGC 3 cut(s) 224, 1119, 2009
Cfr10I RCCGGY 1 cut(s) 12
Cfr13I GGNCC 6 cut(s) 327, 890, 939, 940, 1519, 3019
ClaI ATCGAT 3 cut(s) 535, 1545, 2511
Csp6I GTAC 7 cut(s) 337, 614, 1276, 1434, 2463, 2517, 2816
CviQI GTAC 7 cut(s) 337, 614, 1276, 1434, 2463, 2517, 2816
DdeI CTNAG 8 cut(s) 1607, 2241, 2258, 2382, 2427, 2797, 3023, 3051
DinI GGCGCC 1 cut(s) 223
DraIII CACNNNGTG 1 cut(s) 3126
DriI GACNNNNNGTC 1 cut(s) 2756
EaeI YGGCCR 2 cut(s) 451, 2737
EagI CGGCCG 1 cut(s) 451
Eam1104I CTCTTC 2 cut(s) 1886, 2113
Eam1105I GACNNNNNGTC 1 cut(s) 2756
EarI CTCTTC 2 cut(s) 1886, 2113
EciI GGCGGA 1 cut(s) 91
Ecl136II GAGCTC 2 cut(s) 922, 1291
EclXI CGGCCG 1 cut(s) 451
Eco130I CCWWGG 6 cut(s) 105, 359, 943, 1735, 1834, 2467
Eco24I GRGCYC 4 cut(s) 924, 943, 1003, 1293
Eco31I GGTCTC 2 cut(s) 965, 1019
Eco32I GATATC 1 cut(s) 1831
Eco47I GGWCC 4 cut(s) 327, 890, 1519, 3019
Eco52I CGGCCG 1 cut(s) 451
Eco53kI GAGCTC 2 cut(s) 922, 1291
Eco57I CTGAAG 3 cut(s) 1191, 2070, 2751
Eco81I CCTNAGG 1 cut(s) 2241
Eco88I CYCGRG 1 cut(s) 1022
EcoICRI GAGCTC 2 cut(s) 922, 1291
EcoO109I RGGNCCY 3 cut(s) 890, 940, 3019
EcoRI GAATTC 1 cut(s) 136
EcoRV GATATC 1 cut(s) 1831
EcoT14I CCWWGG 6 cut(s) 105, 359, 943, 1735, 1834, 2467
EcoT38I GRGCYC 4 cut(s) 924, 943, 1003, 1293
EgeI GGCGCC 1 cut(s) 223
EheI GGCGCC 1 cut(s) 223
ErhI CCWWGG 6 cut(s) 105, 359, 943, 1735, 1834, 2467
Esp3I CGTCTC 1 cut(s) 1025
FalI AAGNNNNNCTT 2 cut(s) 2116, 2148
FaqI GGGAC 3 cut(s) 876, 2309, 3353
FbaI TGATCA 1 cut(s) 810
FblI GTMKAC 2 cut(s) 2395, 2749
FokI GGATG 9 cut(s) 266, 521, 623, 820, 991, 1215, 2080, 2365, 2693
FriOI GRGCYC 4 cut(s) 924, 943, 1003, 1293
FspBI CTAG 2 cut(s) 360, 407
GlaI GCGC 3 cut(s) 223, 1118, 2008
GsaI CCCAGC 1 cut(s) 439
GsuI CTGGAG 2 cut(s) 1731, 2499
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 6 cut(s) 16, 453, 941, 1564, 2246, 2739
HapII CCGG 8 cut(s) 13, 225, 454, 544, 751, 996, 1297, 1888
HhaI GCGC 3 cut(s) 224, 1119, 2009
Hin1I GRCGYC 1 cut(s) 222
Hin6I GCGC 3 cut(s) 222, 1117, 2007
HinP1I GCGC 3 cut(s) 222, 1117, 2007
HincII GTYRAC 3 cut(s) 580, 2750, 3118
HindII GTYRAC 3 cut(s) 580, 2750, 3118
HindIII AAGCTT 1 cut(s) 2336
HpaI GTTAAC 1 cut(s) 580
HpaII CCGG 8 cut(s) 13, 225, 454, 544, 751, 996, 1297, 1888
HphI GGTGA 2 cut(s) 635, 1915
Hpy166II GTNNAC 8 cut(s) 580, 1436, 1531, 1645, 2396, 2546, 2750, 3118
Hpy188I TCNGA 8 cut(s) 217, 541, 1171, 1708, 2089, 2770, 2792, 2931
Hpy8I GTNNAC 8 cut(s) 580, 1436, 1531, 1645, 2396, 2546, 2750, 3118
HpyAV CCTTC 8 cut(s) 39, 212, 229, 601, 1153, 1625, 3047, 3354
HpyCH4III ACNGT 6 cut(s) 378, 882, 1930, 2443, 2462, 2543
HpyCH4IV ACGT 2 cut(s) 154, 1808
HpyF3I CTNAG 8 cut(s) 1607, 2241, 2258, 2382, 2427, 2797, 3023, 3051
HpySE526I ACGT 2 cut(s) 154, 1808
Hsp92I GRCGYC 1 cut(s) 222
HspAI GCGC 3 cut(s) 222, 1117, 2007
KasI GGCGCC 1 cut(s) 221
Kpn2I TCCGGA 1 cut(s) 995
KpnI GGTACC 1 cut(s) 2520
Ksp22I TGATCA 1 cut(s) 810
KspAI GTTAAC 1 cut(s) 580
LmnI GCTCC 7 cut(s) 206, 998, 998, 1288, 2614, 2952, 3062
Lsp1109I GCAGC 9 cut(s) 660, 1087, 1679, 1759, 2132, 2134, 2388, 2967, 3296
LweI GCATC 4 cut(s) 499, 541, 2401, 3325
MaeI CTAG 2 cut(s) 360, 407
MaeII ACGT 2 cut(s) 154, 1808
MaeIII GTNAC 7 cut(s) 493, 686, 1693, 1883, 1972, 3068, 3106
MfeI CAATTG 2 cut(s) 1523, 2319
MflI RGATCY 3 cut(s) 1591, 3202, 3296
MlsI TGGCCA 1 cut(s) 2739
MluNI TGGCCA 1 cut(s) 2739
Mly113I GGCGCC 1 cut(s) 222
MlyI GAGTC 7 cut(s) 743, 1024, 1694, 1751, 2246, 2396, 3205
MmeI TCCRAC 5 cut(s) 564, 921, 2701, 2707, 3251
Mox20I TGGCCA 1 cut(s) 2739
MroI TCCGGA 1 cut(s) 995
MroXI GAANNNNTTC 1 cut(s) 378
MscI TGGCCA 1 cut(s) 2739
MseI TTAA 8 cut(s) 504, 579, 1407, 2135, 2366, 2370, 2504, 3234
MslI CAYNNNNRTG 7 cut(s) 273, 291, 1283, 1961, 2025, 2420, 2441
Msp20I TGGCCA 1 cut(s) 2739
MspA1I CMGCKG 1 cut(s) 439
MspI CCGG 8 cut(s) 13, 225, 454, 544, 751, 996, 1297, 1888
MspR9I CCNGG 4 cut(s) 226, 455, 751, 1298
MunI CAATTG 2 cut(s) 1523, 2319
Mva1269I GAATGC 2 cut(s) 854, 2881
MvnI CGCG 3 cut(s) 1119, 1661, 2007
NarI GGCGCC 1 cut(s) 222
NciI CCSGG 4 cut(s) 226, 455, 751, 1298
NcoI CCATGG 1 cut(s) 105
NmeAIII GCCGAG 1 cut(s) 2627
NmuCI GTSAC 3 cut(s) 686, 1883, 3068
NspI RCATGY 3 cut(s) 1643, 2962, 3284
OliI CACNNNNGTG 1 cut(s) 2441
PacI TTAATTAA 1 cut(s) 2370
PaeR7I CTCGAG 1 cut(s) 1022
PagI TCATGA 2 cut(s) 1257, 1317
PciI ACATGT 1 cut(s) 1639
PctI GAATGC 2 cut(s) 854, 2881
PdmI GAANNNNTTC 1 cut(s) 378
PfeI GAWTC 5 cut(s) 83, 110, 212, 2730, 2793
PflFI GACNNNGTC 1 cut(s) 2443
PflMI CCANNNNNTGG 1 cut(s) 2842
PleI GAGTC 7 cut(s) 742, 1023, 1694, 1750, 2246, 2395, 3205
PluTI GGCGCC 1 cut(s) 225
PpsI GAGTC 7 cut(s) 742, 1023, 1694, 1750, 2246, 2395, 3205
PpuMI RGGWCCY 2 cut(s) 890, 3019
PscI ACATGT 1 cut(s) 1639
PsiI TTATAA 2 cut(s) 1191, 1491
Psp124BI GAGCTC 2 cut(s) 924, 1293
Psp5II RGGWCCY 2 cut(s) 890, 3019
PspFI CCCAGC 1 cut(s) 435
PspOMI GGGCCC 1 cut(s) 939
PspPI GGNCC 6 cut(s) 327, 890, 939, 940, 1519, 3019
PspPPI RGGWCCY 2 cut(s) 890, 3019
PstI CTGCAG 1 cut(s) 2269
PsuI RGATCY 3 cut(s) 1591, 3202, 3296
PsyI GACNNNGTC 1 cut(s) 2443
PvuII CAGCTG 1 cut(s) 439
RsaI GTAC 7 cut(s) 338, 615, 1277, 1435, 2464, 2518, 2817
RsaNI GTAC 7 cut(s) 337, 614, 1276, 1434, 2463, 2517, 2816
RseI CAYNNNNRTG 7 cut(s) 273, 291, 1283, 1961, 2025, 2420, 2441
SacI GAGCTC 2 cut(s) 924, 1293
SalI GTCGAC 1 cut(s) 2748
SaqAI TTAA 8 cut(s) 504, 579, 1407, 2135, 2366, 2370, 2504, 3234
Sau96I GGNCC 6 cut(s) 327, 890, 939, 940, 1519, 3019
ScaI AGTACT 1 cut(s) 338
SchI GAGTC 7 cut(s) 743, 1024, 1694, 1751, 2246, 2396, 3205
ScrFI CCNGG 4 cut(s) 226, 455, 751, 1298
SfaNI GCATC 4 cut(s) 499, 541, 2401, 3325
SfcI CTRYAG 3 cut(s) 523, 1150, 2265
SfoI GGCGCC 1 cut(s) 223
Sfr274I CTCGAG 1 cut(s) 1022
SinI GGWCC 4 cut(s) 327, 890, 1519, 3019
SlaI CTCGAG 1 cut(s) 1022
SmiMI CAYNNNNRTG 7 cut(s) 273, 291, 1283, 1961, 2025, 2420, 2441
SmlI CTYRAG 4 cut(s) 923, 1022, 2549, 3190
SmoI CTYRAG 4 cut(s) 923, 1022, 2549, 3190
SsiI CCGC 5 cut(s) 102, 190, 330, 1776, 1779
SspDI GGCGCC 1 cut(s) 221
SspI AATATT 1 cut(s) 838
SspMI CTAG 2 cut(s) 360, 407
SstI GAGCTC 2 cut(s) 924, 1293
StyD4I CCNGG 4 cut(s) 224, 453, 749, 1296
StyI CCWWGG 6 cut(s) 105, 359, 943, 1735, 1834, 2467
TaaI ACNGT 6 cut(s) 378, 882, 1930, 2443, 2462, 2543
TaiI ACGT 2 cut(s) 157, 1811
TaqI TCGA 8 cut(s) 535, 910, 1023, 1182, 1545, 2511, 2699, 2749
TatI WGTACW 3 cut(s) 336, 613, 1275
TauI GCSGC 2 cut(s) 1778, 1781
TfiI GAWTC 5 cut(s) 83, 110, 212, 2730, 2793
Tru1I TTAA 8 cut(s) 504, 579, 1407, 2135, 2366, 2370, 2504, 3234
Tru9I TTAA 8 cut(s) 504, 579, 1407, 2135, 2366, 2370, 2504, 3234
TseFI GTSAC 3 cut(s) 686, 1883, 3068
TseI GCWGC 9 cut(s) 673, 1100, 1667, 1772, 2120, 2147, 2376, 2955, 3284
Tsp45I GTSAC 3 cut(s) 686, 1883, 3068
TspGWI ACGGA 5 cut(s) 157, 559, 1436, 2413, 2960
Tth111I GACNNNGTC 1 cut(s) 2443
Van91I CCANNNNNTGG 1 cut(s) 2842
VneI GTGCAC 2 cut(s) 1529, 1643
VpaK11BI GGWCC 4 cut(s) 327, 890, 1519, 3019
XapI RAATTY 7 cut(s) 136, 364, 739, 1457, 1874, 2090, 2401
XceI RCATGY 3 cut(s) 1643, 2962, 3284
XcmI CCANNNNNNNNNTGG 3 cut(s) 103, 472, 2474
XhoI CTCGAG 1 cut(s) 1022
XmaJI CCTAGG 1 cut(s) 359
XmiI GTMKAC 2 cut(s) 2395, 2749
XmnI GAANNNNTTC 1 cut(s) 378
XspI CTAG 2 cut(s) 360, 407
ZrmI AGTACT 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.