Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0012183

Physical Location & Seq

Genomic Coordinates Reverse (-)

46687787 .. 46692097

Gene Structure 4311 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00319160.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3375 bp

ATGACCTCAAACCGGCCTCTTTTGATCCCGTCTCCAAGAACACCCAACACCCACGATCTCCCAACTCTACCTGTATTTCCCGATTCAGCAAAACCCAGTTCCGCCATGGATTCTGAAAACCCACAGCCCACTTTGAATTCTTCTTCTTCAAGACGTAGCATTTCTTCCGTTCAATCCAAGCCGTCCATGCGGTATGGTTCCAGAGGAGCTGATTCCGAAGCCGCCGCGTCGCAGAAGGAAATGAACGATGAGGATGTCAGGATCATACACATCAATGATCTTGCCCACACCAATGAGAGGTTTGAGTTTTCTGGCAATTCTATAAGGACCACAAAGTACTCAGTTATCACTTTCTTGCCTAGGAATTTGTTTGAACAGTTTCATAGAGTAGCTTATATGTATTTTCTAGTGATTGCTGTGCTCAATCAGCTCCCCCAGCTGGCAGTTTTCGGCCGGGGAGTTTCCATTTTGCCATTAGCATTTGTGTTGTTAGTTACTGCTGTTAAGGATGCGTATGAGGACTTTAGGAGGCATCGATCCGACCGGATTGAGAACAATCGCCTTGCTTCGGTTTTTGTTAACAATCAGTTTCAGTTGAAGAAATGGAAGGATGTACAAGTTGGTGAAATCATCAAAATTGAAGCAAATGAGGGTATTCCTTGTGATATGGTTCTGCTCTCCACCAGTGACCCAACTGGTGTTGCTTATGTGCAGACTATCAATTTAGATGGGGAGTCAAATTTGAAGACCCGGTATGCGAAACAAGAAACCCTCTCCAAGTTGCCTGAAAAGGACAGGATTACTGGGATGATCAAGTGTGAGAAACCCAACAGGAATATTTATGGATTTCAGGCATTCATGGAGATTGATGAGAAAAGACTGTCATTAGGCCCCTCCAACATTGTGCTTCGAGGCTGTGAGCTCAAGAATACTCATTGGGCACTCGGGGTGGCTGTATATGCTGGTCGTGAGACCAAAGTTATGCTGAATAGCTCCGGAGCCCCATCCAAGAGGAGTCGTCTCGAGACCCGTATGAACCACGAGATCATCATGCTCTCGCTGTTTCTTGTTGTCTTGTGTTCAGTTGTCTCTCTGTGTGCTGCTGTTTGGTTGAGGCGCGAAGATGATAATTTGGACGACATACTTTTCTACAGGAAGAAGGACTACTCTGAAGCTGAAGTCAAGGATTATAAGTATTATGGATGGGGATTAGAGATTGTTTTCACATTCCTCATGTCTATCATTGTGTTCCAGATCATGATCCCCATCTCTTTGTACATTTCTATGGAGCTCGTCCGGGTGGGTCAGGCTTACTTCATGATACGGGATAGTCAAATGTATGATGAGGCTTCTAATGCAAGATTTCAGTGTAGAGCTTTGAATATAAATGAAGATTTAGGACAAATTAAGTATGTATTCTCGGACAAAACTGGTACACTTACCGAGAACAAAATGGAATTTCAATGTGCAAGCATTTGGGGAGTTGATTATAATGGTGGGAGAGGCAGTTTGGAAAAGGACCAATTGGTGCACAATGTTCAAATCGATGGAATTGTTTTGAGGCCAAAGATGAATGTAAAGGCTGACCCAGATCTTCTACGACTACTAAGAAGTGAAAAAGACACAAATGAAGGCAAACATGTGCACGAGTTCTTCCTCGCGTTGGCAGCTTGTAATACTATTGTGCCTCTTGTTACAGACTCGTCTGATCCAAATGTGAGATTGATTGATTACCAAGGGGAGTCTCCAGATGAACAGGCATTAGTTTATGCTGCCGCCGCCTATGGTTTTATGCTTATTGAAAGAACGTCTGGTCATATAACTATTGATATCCAAGGAGAAAGGCAAAGGTTCAGTGTTCTGGGTTTGCATGAATTTGATAGTGACCGGAAGAGAATGTCGGTGATATTGGGGTGCCCTGATAAAACCGTGAAAGTGTTTGTAAAAGGTGCTGACACTACCATGTTCAGTGTAACAGACAGAAGATTGAACTTGAACATAATACGCGCAACAGAAGCCCATATTCATGCTTACTCCTCTCTGGGTTTGAGAACACTTGTTGTGGGGATGAGAGAACTCACTGCTTCAGAATTTGAGCAGTGGCACTCTTCTTTTGAGGCAGCAAGCACTGCTTTAATAGGCAGGGCTGCTCTGCTTCGCAAGGTTGCTGGCAACATAGAGAACAATCTCATAATTTTAGGTGCCTCTGGTATTGAAGACAAACTGCAACTGGGGGTGCCTGAGGCCATATACTCTCTAAGAACTGCAGGGGTTCAGGTGTGGGTTTTGACAGGGGACAAGCAAGAAACTGCCATATCAATTGGCTACTCATCAAAGCTTCTTACGAGGAGGATGACCCAGATTTTAATTAACAGCAGCTCTAAGGAGTCATGTAGACGGAATTTAGAAGATGCCATACTCATGTCTAAGAAGCTCACAACCGTGTCTGGCAATGAACACAGTACCCGAGGAAGTTCTGGAGATGGTATAACTTCACTAGCTTTAATTATTGATGGTACCAGCCTTGTTTATATTCTTGACAGTGAACTTGAGGAAAAGCTATTTGAATTAGCAAATAGATGTTCTGTGGTGCTCTGTTGCCGAGTGGCTCCTCTGCAAAAAGCTGGAATTGTTGCCCTTGTAAAAAAAAGAACTCCTGATATGACACTTGCCATTGGGGATGGTGCTAATGATGTTTCGATGATCCAAATGGCTGATGTTGGAGTTGGAATCAGTGGCCAAGAGGGTCGACAAGCTGTCATGGCTTCAGATTTTGCAATGGGGCAGTTCAGATTCTTAGTTCCCCTCTTATTGGTACATGGGCATTGGAATTACCAGCGAATGGGCTACATGATACTTTACAACTTTTATAGGAATGCTGTGTTTGTTTTTATTTTGTTTTGGTATGTGCTCTTTACTTGTTTTACTCTGACAACTGCAATCACGGAATGGAGCAGCATGTTGTATTCTATAATCTACACTGCATTACCTACAATAGTTGTTGGGGTTCTTGACAAGGACCTAAGTAGAATGACCCTTCTATCATATCCTCAGCTATATGGAGCTGGTCACAGACAAGAGTGCTATAACACGAAATTATTTTGGGTAACAATGGTTGACACATTGTGGCAAAGTGTGGCTGTCTTCTTTATCCCCCTTTTTGCATATTGGGGTAGCACCATTGATACCTCAAGTATAGGAGATCTTTGGACTCTTTCGGTGGTTATATTGGTTAATATACACTTGGCTATGGATATTATTCAGTGGAGTTGGATTACACATGCAGCTATTTGGGGATCTATCATTGCAACTTGGATTTGTGTCATTGTAATTGATGCTATACCTTCACTGGTTGGCTACTGGTATGTCCCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0008150 GO:0009987 GO:0010876 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0033036 GO:0034204 GO:0042623 GO:0043492 GO:0044464 GO:0045332 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1124

Amino Acids

126.3

Weight (kDa)

6.44

Isoelectric Point (pI)

38.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	89 - 155	5.9e-25	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	552 - 743	3.7e-14	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	920 - 1116	5.7e-65	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0012183)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04930
fragaria_vesca	FvH4_7g05080 FvH4_7g05080
malus_domestica	MD02G1263000.v1.1 MD07G1054900.v1.1
prunus_persica	Prupe.2G061800_v2.0.a1
pyrus_communis	pycom02g22540 pycom07g04270
rosa_chinensis	RchiOBHm_Chr1g0331371
rosa_laevigata	RLG00000029738
rosa_multiflora	Rmu_sc0005956.1_g000004
rosa_roxburghii	Rroxscaffold_4G00319160
rosa_rugosa	Rorug01G0092900 Rorug01G0092900
rosa_samantha	Rh1AG117900 Rh1BG088700 Rh1CG112800
rosa_wichuraiana	Rw0G002870 Rw1G009440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1191, 1491
Acc65I	GGTACC	1 cut(s)	2516
AccB1I	GGYRCC	4 cut(s)	1914, 2201, 2236, 2516
AccB7I	CCANNNNNTGG	1 cut(s)	2842
AccI	GTMKAC	2 cut(s)	2395, 2749
AccII	CGCG	4 cut(s)	227, 1119, 1661, 2007
AccIII	TCCGGA	1 cut(s)	995
AciI	CCGC	6 cut(s)	102, 190, 222, 225, 1776, 1779
AclWI	GGATC	7 cut(s)	19, 269, 531, 1255, 1703, 2698, 3304
AcoI	YGGCCR	2 cut(s)	451, 2737
AcsI	RAATTY	7 cut(s)	136, 364, 739, 1457, 1874, 2090, 2401
AcuI	CTGAAG	4 cut(s)	1191, 1197, 2070, 2751
AdeI	CACNNNGTG	1 cut(s)	3126
AfaI	GTAC	7 cut(s)	338, 615, 1277, 1435, 2464, 2518, 2817
AfiI	CCNNNNNNNGG	7 cut(s)	12, 297, 439, 568, 1663, 2812, 2842
AflIII	ACRYGT	1 cut(s)	1639
AhdI	GACNNNNNGTC	1 cut(s)	2756
AleI	CACNNNNGTG	1 cut(s)	2441
Alw21I	GWGCWC	7 cut(s)	423, 924, 1293, 1533, 1647, 2595, 2913
Alw26I	GTCTC	6 cut(s)	36, 965, 1019, 1025, 1093, 1749
Alw44I	GTGCAC	2 cut(s)	1529, 1643
AlwI	GGATC	7 cut(s)	19, 269, 531, 1255, 1703, 2698, 3304
Ama87I	CYCGRG	3 cut(s)	944, 1022, 2466
Aor13HI	TCCGGA	1 cut(s)	995
AoxI	GGCC	6 cut(s)	14, 451, 889, 1562, 2244, 2737
ApaLI	GTGCAC	2 cut(s)	1529, 1643
ApeKI	GCWGC	8 cut(s)	1100, 1667, 1772, 2120, 2147, 2376, 2955, 3284
ApoI	RAATTY	7 cut(s)	136, 364, 739, 1457, 1874, 2090, 2401
Asp700I	GAANNNNTTC	1 cut(s)	378
Asp718I	GGTACC	1 cut(s)	2516
AspA2I	CCTAGG	1 cut(s)	359
AspLEI	GCGC	2 cut(s)	1119, 2009
AspS9I	GGNCC	4 cut(s)	327, 890, 1519, 3019
AsuC2I	CCSGG	3 cut(s)	455, 751, 1298
AsuHPI	GGTGA	2 cut(s)	635, 1915
AvaI	CYCGRG	3 cut(s)	944, 1022, 2466
AvaII	GGWCC	3 cut(s)	327, 1519, 3019
AvrII	CCTAGG	1 cut(s)	359
AxyI	CCTNAGG	1 cut(s)	2241
BaeGI	GKGCMC	4 cut(s)	943, 1533, 1647, 1919
BalI	TGGCCA	1 cut(s)	2739
BanI	GGYRCC	4 cut(s)	1914, 2201, 2236, 2516
BanII	GRGCYC	3 cut(s)	924, 1003, 1293
BarI	GAAGNNNNNNTAC	6 cut(s)	148, 180, 1148, 1180, 2400, 2432
BauI	CACGAG	2 cut(s)	1040, 1646
BbsI	GAAGAC	3 cut(s)	752, 2223, 3136
Bbv12I	GWGCWC	7 cut(s)	423, 924, 1293, 1533, 1647, 2595, 2913
BbvCI	CCTCAGC	1 cut(s)	3051
BbvI	GCAGC	8 cut(s)	1087, 1679, 1759, 2132, 2134, 2388, 2967, 3296
BccI	CCATC	8 cut(s)	722, 1012, 1197, 1274, 1541, 2477, 2507, 2675
BceAI	ACGGC	1 cut(s)	166
BclI	TGATCA	1 cut(s)	810
BcnI	CCSGG	3 cut(s)	455, 751, 1298
BcoDI	GTCTC	6 cut(s)	36, 965, 1019, 1025, 1093, 1749
BfaI	CTAG	3 cut(s)	360, 407, 2498
BfmI	CTRYAG	2 cut(s)	1150, 2265
BglII	AGATCT	2 cut(s)	1591, 3202
BlnI	CCTAGG	1 cut(s)	359
BmcAI	AGTACT	1 cut(s)	338
Bme1390I	CCNGG	3 cut(s)	455, 751, 1298
Bme18I	GGWCC	3 cut(s)	327, 1519, 3019
BmeRI	GACNNNNNGTC	1 cut(s)	2756
BmeT110I	CYCGRG	3 cut(s)	944, 1022, 2466
BmgT120I	GGNCC	4 cut(s)	327, 890, 1519, 3019
BmiI	GGNNCC	8 cut(s)	199, 892, 1000, 1916, 2203, 2238, 2518, 2610
BmrFI	CCNGG	3 cut(s)	455, 751, 1298
BmrI	ACTGGG	3 cut(s)	90, 813, 2240
BmsI	GCATC	4 cut(s)	499, 541, 2401, 3325
BmuI	ACTGGG	3 cut(s)	90, 813, 2240
BpiI	GAAGAC	3 cut(s)	752, 2223, 3136
BpmI	CTGGAG	2 cut(s)	1731, 2499
Bpu10I	CCTNAGC	1 cut(s)	3051
BpuEI	CTTGAG	3 cut(s)	908, 2570, 3175
BpuMI	CCSGG	3 cut(s)	455, 751, 1298
Bsa29I	ATCGAT	2 cut(s)	535, 1545
BsaBI	GATNNNNATC	2 cut(s)	1259, 1265
BsaI	GGTCTC	2 cut(s)	965, 1019
BsaJI	CCNNGG	6 cut(s)	105, 359, 454, 1735, 1834, 2467
BsaWI	WCCGGW	3 cut(s)	543, 995, 1887
BsaXI	ACNNNNNCTCC	2 cut(s)	1492, 1522
Bsc4I	CCNNNNNNNGG	7 cut(s)	12, 297, 439, 568, 1663, 2812, 2842
Bse118I	RCCGGY	1 cut(s)	12
Bse1I	ACTGG	8 cut(s)	96, 684, 700, 808, 1435, 2235, 3354, 3365
Bse21I	CCTNAGG	1 cut(s)	2241
Bse3DI	GCAATG	3 cut(s)	2458, 2784, 3303
Bse8I	GATNNNNATC	2 cut(s)	1259, 1265
BseAI	TCCGGA	1 cut(s)	995
BseCI	ATCGAT	2 cut(s)	535, 1545
BseDI	CCNNGG	6 cut(s)	105, 359, 454, 1735, 1834, 2467
BseGI	GGATG	9 cut(s)	259, 514, 616, 813, 1004, 1208, 2073, 2358, 2686
BseJI	GATNNNNATC	2 cut(s)	1259, 1265
BseLI	CCNNNNNNNGG	7 cut(s)	12, 297, 439, 568, 1663, 2812, 2842
BseMI	GCAATG	3 cut(s)	2458, 2784, 3303
BseMII	CTCAG	3 cut(s)	354, 2232, 3065
BseNI	ACTGG	8 cut(s)	96, 684, 700, 808, 1435, 2235, 3354, 3365
BseRI	GAGGAG	5 cut(s)	219, 1027, 2026, 2362, 2601
BseSI	GKGCMC	4 cut(s)	943, 1533, 1647, 1919
BseX3I	CGGCCG	1 cut(s)	451
BseXI	GCAGC	8 cut(s)	1087, 1679, 1759, 2132, 2134, 2388, 2967, 3296
BseYI	CCCAGC	1 cut(s)	435
BsgI	GTGCAG	1 cut(s)	731
Bsh1236I	CGCG	4 cut(s)	227, 1119, 1661, 2007
Bsh1285I	CGRYCG	2 cut(s)	454, 544
BshFI	GGCC	6 cut(s)	16, 453, 891, 1564, 2246, 2739
BshNI	GGYRCC	4 cut(s)	1914, 2201, 2236, 2516
BshVI	ATCGAT	2 cut(s)	535, 1545
BsiEI	CGRYCG	2 cut(s)	454, 544
BsiHKAI	GWGCWC	7 cut(s)	423, 924, 1293, 1533, 1647, 2595, 2913
BsiHKCI	CYCGRG	3 cut(s)	944, 1022, 2466
BsiSI	CCGG	7 cut(s)	13, 454, 544, 751, 996, 1297, 1888
BslFI	GGGAC	2 cut(s)	2309, 3353
BslI	CCNNNNNNNGG	7 cut(s)	12, 297, 439, 568, 1663, 2812, 2842
BsmAI	GTCTC	6 cut(s)	36, 965, 1019, 1025, 1093, 1749
BsmBI	CGTCTC	2 cut(s)	36, 1025
BsmFI	GGGAC	2 cut(s)	2309, 3353
BsmI	GAATGC	2 cut(s)	854, 2881
BsnI	GGCC	6 cut(s)	16, 453, 891, 1564, 2246, 2739
Bso31I	GGTCTC	2 cut(s)	965, 1019
BsoBI	CYCGRG	3 cut(s)	944, 1022, 2466
Bsp13I	TCCGGA	1 cut(s)	995
Bsp1407I	TGTACA	2 cut(s)	613, 1275
Bsp19I	CCATGG	1 cut(s)	105
BspACI	CCGC	6 cut(s)	102, 190, 222, 225, 1776, 1779
BspANI	GGCC	6 cut(s)	16, 453, 891, 1564, 2246, 2739
BspCNI	CTCAG	3 cut(s)	353, 2233, 3064
BspDI	ATCGAT	2 cut(s)	535, 1545
BspEI	TCCGGA	1 cut(s)	995
BspFNI	CGCG	4 cut(s)	227, 1119, 1661, 2007
BspHI	TCATGA	2 cut(s)	1257, 1317
BspLI	GGNNCC	8 cut(s)	199, 892, 1000, 1916, 2203, 2238, 2518, 2610
BspMAI	CTGCAG	1 cut(s)	2269
BspPI	GGATC	7 cut(s)	19, 269, 531, 1255, 1703, 2698, 3304
BspT107I	GGYRCC	4 cut(s)	1914, 2201, 2236, 2516
BspTNI	GGTCTC	2 cut(s)	965, 1019
BsrDI	GCAATG	3 cut(s)	2458, 2784, 3303
BsrFI	RCCGGY	1 cut(s)	12
BsrGI	TGTACA	2 cut(s)	613, 1275
BsrI	ACTGG	8 cut(s)	96, 684, 700, 808, 1435, 2235, 3354, 3365
BssAI	RCCGGY	1 cut(s)	12
BssECI	CCNNGG	6 cut(s)	105, 359, 454, 1735, 1834, 2467
BssSI	CACGAG	2 cut(s)	1040, 1646
BssT1I	CCWWGG	4 cut(s)	105, 359, 1735, 1834
Bst2BI	CACGAG	2 cut(s)	1040, 1646
Bst4CI	ACNGT	6 cut(s)	378, 882, 1930, 2443, 2462, 2543
Bst6I	CTCTTC	2 cut(s)	1886, 2113
BstAPI	GCANNNNNTGC	1 cut(s)	2129
BstAUI	TGTACA	2 cut(s)	613, 1275
BstC8I	GCNNGC	4 cut(s)	441, 1471, 2125, 2170
BstDEI	CTNAG	9 cut(s)	340, 1607, 2241, 2258, 2382, 2427, 2797, 3023, 3051
BstDSI	CCRYGG	1 cut(s)	105
BstF5I	GGATG	9 cut(s)	259, 514, 616, 813, 1004, 1208, 2073, 2358, 2686
BstFNI	CGCG	4 cut(s)	227, 1119, 1661, 2007
BstHHI	GCGC	2 cut(s)	1119, 2009
BstMAI	GTCTC	6 cut(s)	36, 965, 1019, 1025, 1093, 1749
BstMCI	CGRYCG	2 cut(s)	454, 544
BstNSI	RCATGY	3 cut(s)	1643, 2962, 3284
BstSCI	CCNGG	3 cut(s)	453, 749, 1296
BstSFI	CTRYAG	2 cut(s)	1150, 2265
BstSLI	GKGCMC	4 cut(s)	943, 1533, 1647, 1919
BstUI	CGCG	4 cut(s)	227, 1119, 1661, 2007
BstV1I	GCAGC	8 cut(s)	1087, 1679, 1759, 2132, 2134, 2388, 2967, 3296
BstV2I	GAAGAC	3 cut(s)	752, 2223, 3136
BstX2I	RGATCY	3 cut(s)	1591, 3202, 3296
BstYI	RGATCY	3 cut(s)	1591, 3202, 3296
BstZI	CGGCCG	1 cut(s)	451
Bsu15I	ATCGAT	2 cut(s)	535, 1545
Bsu36I	CCTNAGG	1 cut(s)	2241
BsuRI	GGCC	6 cut(s)	16, 453, 891, 1564, 2246, 2739
BsuTUI	ATCGAT	2 cut(s)	535, 1545
BtgI	CCRYGG	1 cut(s)	105
BtsCI	GGATG	9 cut(s)	259, 514, 616, 813, 1004, 1208, 2073, 2358, 2686
BtsI	GCAGTG	4 cut(s)	2079, 2105, 2127, 2979
Cac8I	GCNNGC	4 cut(s)	441, 1471, 2125, 2170
CciI	TCATGA	2 cut(s)	1257, 1317
CfoI	GCGC	2 cut(s)	1119, 2009
Cfr10I	RCCGGY	1 cut(s)	12
Cfr13I	GGNCC	4 cut(s)	327, 890, 1519, 3019
ClaI	ATCGAT	2 cut(s)	535, 1545
CseI	GACGC	1 cut(s)	216
Csp6I	GTAC	7 cut(s)	337, 614, 1276, 1434, 2463, 2517, 2816
CviQI	GTAC	7 cut(s)	337, 614, 1276, 1434, 2463, 2517, 2816
DdeI	CTNAG	9 cut(s)	340, 1607, 2241, 2258, 2382, 2427, 2797, 3023, 3051
DraIII	CACNNNGTG	1 cut(s)	3126
DriI	GACNNNNNGTC	1 cut(s)	2756
EaeI	YGGCCR	2 cut(s)	451, 2737
EagI	CGGCCG	1 cut(s)	451
Eam1104I	CTCTTC	2 cut(s)	1886, 2113
Eam1105I	GACNNNNNGTC	1 cut(s)	2756
EarI	CTCTTC	2 cut(s)	1886, 2113
EciI	GGCGGA	1 cut(s)	91
Ecl136II	GAGCTC	2 cut(s)	922, 1291
EclXI	CGGCCG	1 cut(s)	451
Eco130I	CCWWGG	4 cut(s)	105, 359, 1735, 1834
Eco24I	GRGCYC	3 cut(s)	924, 1003, 1293
Eco31I	GGTCTC	2 cut(s)	965, 1019
Eco32I	GATATC	1 cut(s)	1831
Eco47I	GGWCC	3 cut(s)	327, 1519, 3019
Eco52I	CGGCCG	1 cut(s)	451
Eco53kI	GAGCTC	2 cut(s)	922, 1291
Eco57I	CTGAAG	4 cut(s)	1191, 1197, 2070, 2751
Eco81I	CCTNAGG	1 cut(s)	2241
Eco88I	CYCGRG	3 cut(s)	944, 1022, 2466
EcoICRI	GAGCTC	2 cut(s)	922, 1291
EcoO109I	RGGNCCY	2 cut(s)	890, 3019
EcoRI	GAATTC	1 cut(s)	136
EcoRV	GATATC	1 cut(s)	1831
EcoT14I	CCWWGG	4 cut(s)	105, 359, 1735, 1834
EcoT38I	GRGCYC	3 cut(s)	924, 1003, 1293
ErhI	CCWWGG	4 cut(s)	105, 359, 1735, 1834
Esp3I	CGTCTC	2 cut(s)	36, 1025
FalI	AAGNNNNNCTT	2 cut(s)	2116, 2148
FaqI	GGGAC	2 cut(s)	2309, 3353
FbaI	TGATCA	1 cut(s)	810
FblI	GTMKAC	2 cut(s)	2395, 2749
FokI	GGATG	9 cut(s)	266, 521, 623, 820, 991, 1215, 2080, 2365, 2693
FriOI	GRGCYC	3 cut(s)	924, 1003, 1293
FspBI	CTAG	3 cut(s)	360, 407, 2498
GlaI	GCGC	2 cut(s)	1118, 2008
GsaI	CCCAGC	1 cut(s)	439
GsuI	CTGGAG	2 cut(s)	1731, 2499
HaeIII	GGCC	6 cut(s)	16, 453, 891, 1564, 2246, 2739
HapII	CCGG	7 cut(s)	13, 454, 544, 751, 996, 1297, 1888
HgaI	GACGC	1 cut(s)	216
HhaI	GCGC	2 cut(s)	1119, 2009
Hin6I	GCGC	2 cut(s)	1117, 2007
HinP1I	GCGC	2 cut(s)	1117, 2007
HincII	GTYRAC	3 cut(s)	580, 2750, 3118
HindII	GTYRAC	3 cut(s)	580, 2750, 3118
HindIII	AAGCTT	1 cut(s)	2336
HpaI	GTTAAC	1 cut(s)	580
HpaII	CCGG	7 cut(s)	13, 454, 544, 751, 996, 1297, 1888
HphI	GGTGA	2 cut(s)	635, 1915
Hpy166II	GTNNAC	8 cut(s)	580, 1436, 1531, 1645, 2396, 2546, 2750, 3118
Hpy8I	GTNNAC	8 cut(s)	580, 1436, 1531, 1645, 2396, 2546, 2750, 3118
Hpy99I	CGWCG	1 cut(s)	232
HpyAV	CCTTC	6 cut(s)	229, 601, 1153, 1625, 3047, 3354
HpyCH4III	ACNGT	6 cut(s)	378, 882, 1930, 2443, 2462, 2543
HpyCH4IV	ACGT	2 cut(s)	154, 1808
HpyF3I	CTNAG	9 cut(s)	340, 1607, 2241, 2258, 2382, 2427, 2797, 3023, 3051
HpySE526I	ACGT	2 cut(s)	154, 1808
HspAI	GCGC	2 cut(s)	1117, 2007
Kpn2I	TCCGGA	1 cut(s)	995
KpnI	GGTACC	1 cut(s)	2520
Ksp22I	TGATCA	1 cut(s)	810
KspAI	GTTAAC	1 cut(s)	580
LmnI	GCTCC	8 cut(s)	206, 435, 998, 998, 1288, 2614, 2952, 3062
Lsp1109I	GCAGC	8 cut(s)	1087, 1679, 1759, 2132, 2134, 2388, 2967, 3296
LweI	GCATC	4 cut(s)	499, 541, 2401, 3325
MaeI	CTAG	3 cut(s)	360, 407, 2498
MaeII	ACGT	2 cut(s)	154, 1808
MaeIII	GTNAC	7 cut(s)	493, 686, 1693, 1883, 1972, 3068, 3106
MfeI	CAATTG	2 cut(s)	1523, 2319
MflI	RGATCY	3 cut(s)	1591, 3202, 3296
MlsI	TGGCCA	1 cut(s)	2739
MluNI	TGGCCA	1 cut(s)	2739
MlyI	GAGTC	6 cut(s)	743, 1024, 1694, 1751, 2396, 3205
MmeI	TCCRAC	5 cut(s)	564, 921, 2701, 2707, 3251
Mox20I	TGGCCA	1 cut(s)	2739
MroI	TCCGGA	1 cut(s)	995
MroXI	GAANNNNTTC	1 cut(s)	378
MscI	TGGCCA	1 cut(s)	2739
MseI	TTAA	8 cut(s)	504, 579, 1407, 2135, 2366, 2370, 2504, 3234
MslI	CAYNNNNRTG	7 cut(s)	273, 291, 1283, 1961, 2025, 2420, 2441
Msp20I	TGGCCA	1 cut(s)	2739
MspA1I	CMGCKG	1 cut(s)	439
MspI	CCGG	7 cut(s)	13, 454, 544, 751, 996, 1297, 1888
MspR9I	CCNGG	3 cut(s)	455, 751, 1298
MunI	CAATTG	2 cut(s)	1523, 2319
Mva1269I	GAATGC	2 cut(s)	854, 2881
MvnI	CGCG	4 cut(s)	227, 1119, 1661, 2007
NciI	CCSGG	3 cut(s)	455, 751, 1298
NcoI	CCATGG	1 cut(s)	105
NlaIV	GGNNCC	8 cut(s)	199, 892, 1000, 1916, 2203, 2238, 2518, 2610
NmeAIII	GCCGAG	1 cut(s)	2627
NmuCI	GTSAC	3 cut(s)	686, 1883, 3068
NspI	RCATGY	3 cut(s)	1643, 2962, 3284
OliI	CACNNNNGTG	1 cut(s)	2441
PacI	TTAATTAA	1 cut(s)	2370
PaeR7I	CTCGAG	1 cut(s)	1022
PagI	TCATGA	2 cut(s)	1257, 1317
PciI	ACATGT	1 cut(s)	1639
PctI	GAATGC	2 cut(s)	854, 2881
PdmI	GAANNNNTTC	1 cut(s)	378
PfeI	GAWTC	5 cut(s)	83, 110, 212, 2730, 2793
PflMI	CCANNNNNTGG	1 cut(s)	2842
PleI	GAGTC	6 cut(s)	742, 1023, 1694, 1750, 2395, 3205
PpsI	GAGTC	6 cut(s)	742, 1023, 1694, 1750, 2395, 3205
PpuMI	RGGWCCY	1 cut(s)	3019
PscI	ACATGT	1 cut(s)	1639
PsiI	TTATAA	2 cut(s)	1191, 1491
Psp124BI	GAGCTC	2 cut(s)	924, 1293
Psp5II	RGGWCCY	1 cut(s)	3019
PspFI	CCCAGC	1 cut(s)	435
PspN4I	GGNNCC	8 cut(s)	199, 892, 1000, 1916, 2203, 2238, 2518, 2610
PspPI	GGNCC	4 cut(s)	327, 890, 1519, 3019
PspPPI	RGGWCCY	1 cut(s)	3019
PstI	CTGCAG	1 cut(s)	2269
PsuI	RGATCY	3 cut(s)	1591, 3202, 3296
PvuII	CAGCTG	1 cut(s)	439
RsaI	GTAC	7 cut(s)	338, 615, 1277, 1435, 2464, 2518, 2817
RsaNI	GTAC	7 cut(s)	337, 614, 1276, 1434, 2463, 2517, 2816
RseI	CAYNNNNRTG	7 cut(s)	273, 291, 1283, 1961, 2025, 2420, 2441
SacI	GAGCTC	2 cut(s)	924, 1293
SalI	GTCGAC	1 cut(s)	2748
SaqAI	TTAA	8 cut(s)	504, 579, 1407, 2135, 2366, 2370, 2504, 3234
Sau96I	GGNCC	4 cut(s)	327, 890, 1519, 3019
ScaI	AGTACT	1 cut(s)	338
SchI	GAGTC	6 cut(s)	743, 1024, 1694, 1751, 2396, 3205
ScrFI	CCNGG	3 cut(s)	455, 751, 1298
SfaNI	GCATC	4 cut(s)	499, 541, 2401, 3325
SfcI	CTRYAG	2 cut(s)	1150, 2265
Sfr274I	CTCGAG	1 cut(s)	1022
SinI	GGWCC	3 cut(s)	327, 1519, 3019
SlaI	CTCGAG	1 cut(s)	1022
SmiMI	CAYNNNNRTG	7 cut(s)	273, 291, 1283, 1961, 2025, 2420, 2441
SmlI	CTYRAG	4 cut(s)	923, 1022, 2549, 3190
SmoI	CTYRAG	4 cut(s)	923, 1022, 2549, 3190
SsiI	CCGC	6 cut(s)	102, 190, 222, 225, 1776, 1779
SspI	AATATT	1 cut(s)	838
SspMI	CTAG	3 cut(s)	360, 407, 2498
SstI	GAGCTC	2 cut(s)	924, 1293
StyD4I	CCNGG	3 cut(s)	453, 749, 1296
StyI	CCWWGG	4 cut(s)	105, 359, 1735, 1834
TaaI	ACNGT	6 cut(s)	378, 882, 1930, 2443, 2462, 2543
TaiI	ACGT	2 cut(s)	157, 1811
TaqI	TCGA	6 cut(s)	535, 910, 1023, 1545, 2699, 2749
TatI	WGTACW	3 cut(s)	336, 613, 1275
TauI	GCSGC	4 cut(s)	224, 227, 1778, 1781
TfiI	GAWTC	5 cut(s)	83, 110, 212, 2730, 2793
Tru1I	TTAA	8 cut(s)	504, 579, 1407, 2135, 2366, 2370, 2504, 3234
Tru9I	TTAA	8 cut(s)	504, 579, 1407, 2135, 2366, 2370, 2504, 3234
TseFI	GTSAC	3 cut(s)	686, 1883, 3068
TseI	GCWGC	8 cut(s)	1100, 1667, 1772, 2120, 2147, 2376, 2955, 3284
Tsp45I	GTSAC	3 cut(s)	686, 1883, 3068
TspGWI	ACGGA	3 cut(s)	157, 2413, 2960
Van91I	CCANNNNNTGG	1 cut(s)	2842
VneI	GTGCAC	2 cut(s)	1529, 1643
VpaK11BI	GGWCC	3 cut(s)	327, 1519, 3019
XapI	RAATTY	7 cut(s)	136, 364, 739, 1457, 1874, 2090, 2401
XceI	RCATGY	3 cut(s)	1643, 2962, 3284
XcmI	CCANNNNNNNNNTGG	1 cut(s)	103
XhoI	CTCGAG	1 cut(s)	1022
XmaJI	CCTAGG	1 cut(s)	359
XmiI	GTMKAC	2 cut(s)	2395, 2749
XmnI	GAANNNNTTC	1 cut(s)	378
XspI	CTAG	3 cut(s)	360, 407, 2498
ZrmI	AGTACT	1 cut(s)	338

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.