Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0012183

Physical Location & Seq

Genomic Coordinates Forward (+)

5074711 .. 5080885

Gene Structure 6175 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1054900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3678 bp

ATGACCTCGAAGCGGCCTTTTCTGATCCCATCTCCAAGAACCCCCAATACCCAACGCCTCCCTACTCTGCCCATCTATTCTGATTTCTTAAATTCCACTTCCGGTGGCACTGGATCGTTTTCCGGCATGAATTCGAGAAACCCAGCTGCAGCTGACAATTCCATCAACGTTGAACCCACCACCCTCAATTCTACCTCCCTTAGAAGCGTTTCTTCCCTTCAATCCAAGGCTTCCCAGAACAATTCTGTTTGGGAAGTGAGTTTCGGCGATGTCGGATCGAAGCCAGTGCGTTATGGATCGAGAGGAGCTGATTCCGAGGCGCTCAGCATGTCCCAGAAGGAGTTGAACGAGGAGAATGTAAGGGTTGTTTATATAGATGATGTTGCTAAGACAAATGAGAGGTTTGCGTTTTCTGGTAATTCAATTAGGACGGCAAAGTACTCAATTTTTACATTTTTGCCTAGAAATTTGTTTGAACAGTTTCATAGGGTAGCATACATATATTTTCTTGTGATTGCTGTGCTCAATCAGCTCCCCCAGCTGGCGGTTTTTGGCCGTGGAGTTTCCATATTGCCATTGGCATTTGTGTTGCTTGTTACTGCTGTTAAGGATGCCTACGAGGACTACAGGCGGCATCGGTCTGATAGAATTGAGAACAATCGTCTGGCATCAGTTTTGGTTGGTAACCAGTTCCAGTCGAAAAAGTGGAAGGATGTTCGAGTTGGTGAAATCATCAAAATTGAAGCAAATGAGGCTATACCTTGTGATATGGTACTGCTCTCAACGAGTGATCCAACTGGGGTTGCTTATGTGCAGACTATCAATTTAGATGGGGAGTCAAATTTGAAGACGAGGTACGCTAAACAAGAAACCCTCTCCAAGTTACCTGATAAGGAGAAGATTACTGGGGTGATTAAGTGTGAAAATCCCAACAGGAACATATACGGCCTTCACGCATTCATGGAAATTGATGGGAAGCGGTTGTCACTGGGACCCTCTAACATTGTACTTCGAGGGTGTGAGCTTAAGAATACGCATTGGGCACTTGGAGTTGCTGTATATGCCGGTCGTGAGACCAAAGTTATGCTCAATAACTCTGGAGCCCCATCCAAGAGGAGTCGCCTTGAGACCCGTATGAACTTTGAGATCATTTTGCTCTCTGGATTTCTTGTTACTTTGTGTACAGTTGTCTCATTATGTGCTGCTGTTTGGTTGAGACGCAACAATGAGAAGTTGGATGACATACTTTTCTACAGGAAGAAGGACTACTCAGAGGGGAAAGTGGACAATTATAACTATTATGGATGGGGTTTAGAGATTATTTTCACGTTCCTTATGTCTGTCATTGTGTTCCAGGTCATGATCCCCATCTCATTGTACATTTCTATGGAGCTTGTCCGGGTGGGTCAGGCTTACTTCATGATCAGGGATGGTCAAATGTATGATGAGGCCTCTAATTCAAGATTTCAGTGCCGAGCTTTGAATATCAACGAAGATTTAGGACAAATTAAGTATGTATTCTCAGACAAAACTGGTACGCTGACAGAAAATAAAATGGAATTTCAATGTGCAAGCATTTGGGGAGTAGATTATAACGGTGGGAGAAGCAGTTTGGAGCAAGTGGGGTACTCTGTTGAAGTTGATGGGAAGATTTTGAGGCCAAAGATGAAGGTGAAGGCTGACCCAGAACTTCAGCAATTACTCAGAAGTGGAAAGAATACAAATGAAGGCAAACATGTGTACGAGTTCTTCCTGGCATTGGCAGCTTGTAATACCATTGTGCCTCTTGTTGTAGACACGTCTGATCCTAATGTGGGATTATTAGATTACCAAGGGGAGTCCCCAGATGAACAAGCATTGGTTTATGCTGCCGCCGCCTATGGTTTTATGCTTATTGAACGAACCTCTGGTCATATAATTATTGATATCCAAGGGGACAGGAAAAGGTTCAATGTCTTGGGTTTGCATGAGTTCGATAGTGATCGGAAGAGAATGTCGGTTATATTGGGGTGCCCTGATAAGACTGTCAAAGTCTTTGTAAAAGGTGCTGACACAACCATGTTCAGTGTAATAGACAAACGATTGAACTTGGATATAATAAGAGCAACAGAAGTCCATATTCATGCTTACTCCTCTTTGGGTTTGAGAACACTTGTGGTGGGCATGCGAGAACTGAGTGCTACAGAATTCGAGCAGTGGCACTTGTCTTTTGAGGAAGCAAGCACTGCTTTAATTGGTAGGGCTGCTCTACTTCGCAATGTTGCGGGCAACATAGAAAACAATCTCATTATACTAGGTGCCTCTGGCATTGAAGATAAACTGCAACTGGGGGTGCCTGAAGCAATAGACTCTCTAAGAACAGCAGGGGTTCAGGTATGGATTTTGACAGGGGACAAGCAAGAAACTGCCATATCAATCGGCTACTCCTCAAAGCTTCTAACAAGAAGAATGACACAGATTATAATTAACAGTAGCTCTAAGGATTCATGTCGAAGGGGTTTAGAGGATGCCGTACTAATGTCTAGGAAGCTCCTGACGGTGTCTGCAGATACACACACCGACGGAGGAAACTCTGGACATGGTGGAACTCAAGTAGCTTTAATTATTGACGGCACCAGCCTTGTGTACATTCTTGACAGTGAACTTGAGGAAAAGCTCTTTGAACTAGCAAGTAACTGTGCTGTGGTGCTTTGTTGCCGAGTGGCTCCATTGCAAAAGGCTGGAATTGTAGCCCTTGTGAAGAACAGGACGACTGATATGACACTTGCCATTGGGGATGGTGCTAATGACGTTTCAATGATCCAAATGGCTGATGTGGGAGTTGGCATCAGTGGCCAAGAAGGTCGGCAAGCTGTCATGGCATCCGATTTTGCAATGGGGCAGTTCAGATTCTTAGTTCCCCTTTTATTGGTACACGGTCATTGGAATTACCAGCGGATGGGCTATATGATATTATACAATTTCTATAGGAATGCAGTGTTTGTTCTGATTTTGTTTTGGTACGTGCTCTTCACTTCTTTTAGTTTGACAACTGCGATCACTGAATGGAGCAGCATGTTGTATTCTATAATTTACACTGCAGCACCTACAATTGTTGTTGGGATTCTTGACAAGGACCTAAGTAGAAGGACACTTCTAGATTACCCGCAGCTATACGGAGCTGGTCAGAGACAAGAATGCTACAACTCAAAATTATTTTGGTTAACGATCGGTGACACATTGTGGCAAAGTCTGGCTGTCTTCTTTATCCCTCTCTTTGCGTATTGGGGCAGCAGCATTGATACATCAAGTATAGGAGATCTTTGGACACTTGCAGTGGTAATTTTGGTTAATTTTCATTTGGCTATGGATGTTATCCGGTGGAATTGGATTACACATGCATCAATTTGGGGATCTATAATTGCGACTTGGATTTGTGTCATTGTCATTGATGCTATACCTTCACTGGTTGGCTACTGGGCCATTTTTCAAGTCGCAAAGACTGCATCGTTTTGGTTATGTTTGTTGGCAATCGTCACAGCTGCAATCGCTCCACGCTTTGTTGTAAAGTTTCTGTATCAGTACTATAGACCTTGTGATGTTCAAATTGCAAGAGAATTTGAGAAGTTCGGCAATCCAAGCGCGTCAAACCCTGCACAAATAGAAATGGATGCGATCTTGGACCTGCGTAGGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004012 GO:0005215 GO:0005319 GO:0005548 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0008150 GO:0009987 GO:0010876 GO:0015711 GO:0015748 GO:0015914 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0033036 GO:0034204 GO:0042623 GO:0043492 GO:0044464 GO:0045332 GO:0051179 GO:0051234 GO:0061024 GO:0065007 GO:0065008 GO:0071702 GO:0071840 GO:0071944 GO:0097035

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase E1-E2_ATPase PhoLip_ATPase_C PhoLip_ATPase_N

Protein Analysis

1226

Amino Acids

136.84

Weight (kDa)

7.21

Isoelectric Point (pI)

36.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PhoLip_ATPase_N	PF16209	123 - 189	1e-23	Phospholipid-translocating ATPase N-terminal
Cation_ATPase	PF13246	586 - 775	2.1e-13	P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C	PF16212	952 - 1191	6.7e-76	Phospholipid-translocating P-type ATPase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012183)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G04930
fragaria_vesca	FvH4_7g05080 FvH4_7g05080
malus_domestica	MD02G1263000.v1.1 MD07G1054900.v1.1
prunus_persica	Prupe.2G061800_v2.0.a1
pyrus_communis	pycom02g22540 pycom07g04270
rosa_chinensis	RchiOBHm_Chr1g0331371
rosa_laevigata	RLG00000029738
rosa_multiflora	Rmu_sc0005956.1_g000004
rosa_roxburghii	Rroxscaffold_4G00319160
rosa_rugosa	Rorug01G0092900 Rorug01G0092900
rosa_samantha	Rh1AG117900 Rh1BG088700 Rh1CG112800
rosa_wichuraiana	Rw0G002870 Rw1G009440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1293, 1593, 2462
Acc36I	ACCTGC	1 cut(s)	3672
AccB1I	GGYRCC	4 cut(s)	2010, 2297, 2332, 2612
AccB7I	CCANNNNNTGG	1 cut(s)	2938
AccI	GTMKAC	1 cut(s)	1794
AccII	CGCG	1 cut(s)	3623
AciI	CCGC	9 cut(s)	13, 545, 631, 979, 1872, 1875, 2264, 2935, 3146
AclI	AACGTT	1 cut(s)	168
AclWI	GGATC	9 cut(s)	19, 121, 283, 304, 785, 1357, 1799, 2794, 3400
AcoI	YGGCCR	2 cut(s)	553, 2833
AcsI	RAATTY	7 cut(s)	91, 130, 466, 841, 1559, 2186, 3596
AcuI	CTGAAG	2 cut(s)	1676, 2358
AdeI	CACNNNGTG	1 cut(s)	3222
AfiI	CCNNNNNNNGG	7 cut(s)	12, 541, 544, 1759, 1814, 2908, 2938
AflII	CTTAAG	1 cut(s)	1025
AflIII	ACRYGT	2 cut(s)	1735, 1797
AjiI	CACGTC	1 cut(s)	1800
AjnI	CCWGG	2 cut(s)	1353, 1752
Alw21I	GWGCWC	2 cut(s)	525, 3009
Alw26I	GTCTC	5 cut(s)	1067, 1121, 1195, 1210, 3163
AlwI	GGATC	9 cut(s)	19, 121, 283, 304, 785, 1357, 1799, 2794, 3400
AoxI	GGCC	7 cut(s)	14, 553, 946, 1449, 1658, 2833, 3459
ApoI	RAATTY	7 cut(s)	91, 130, 466, 841, 1559, 2186, 3596
ArsI	GACNNNNNNTTYG	2 cut(s)	1894, 1926
Asp700I	GAANNNNTTC	3 cut(s)	208, 480, 1946
AspLEI	GCGC	2 cut(s)	322, 3623
AspS9I	GGNCC	4 cut(s)	992, 3115, 3459, 3661
AsuC2I	CCSGG	1 cut(s)	1400
AsuHPI	GGTGA	4 cut(s)	737, 922, 1684, 3224
AvaII	GGWCC	3 cut(s)	992, 3115, 3661
BaeGI	GKGCMC	2 cut(s)	1045, 2015
BalI	TGGCCA	1 cut(s)	2835
BanI	GGYRCC	4 cut(s)	2010, 2297, 2332, 2612
BanII	GRGCYC	1 cut(s)	1105
BarI	GAAGNNNNNNTAC	8 cut(s)	839, 871, 1250, 1282, 1733, 1765, 2993, 3025
BbsI	GAAGAC	2 cut(s)	854, 3232
Bbv12I	GWGCWC	2 cut(s)	525, 3009
BceAI	ACGGC	5 cut(s)	447, 540, 961, 2495, 2626
BcgI	CGANNNNNNTGC	2 cut(s)	268, 302
BciT130I	CCWGG	2 cut(s)	1355, 1754
BclI	TGATCA	1 cut(s)	1422
BcnI	CCSGG	1 cut(s)	1400
BcoDI	GTCTC	5 cut(s)	1067, 1121, 1195, 1210, 3163
BfaI	CTAG	5 cut(s)	462, 2294, 2523, 2666, 3137
BfmI	CTRYAG	8 cut(s)	147, 625, 1252, 2181, 2544, 2965, 3078, 3565
BfoI	RGCGCY	1 cut(s)	323
BfrI	CTTAAG	1 cut(s)	1025
BfuAI	ACCTGC	1 cut(s)	3672
BglII	AGATCT	1 cut(s)	3298
BlpI	GCTNAGC	1 cut(s)	323
BmcAI	AGTACT	2 cut(s)	440, 3563
Bme1390I	CCNGG	3 cut(s)	1355, 1400, 1754
Bme18I	GGWCC	3 cut(s)	992, 3115, 3661
BmgBI	CACGTC	1 cut(s)	1800
BmgT120I	GGNCC	4 cut(s)	992, 3115, 3459, 3661
BmiI	GGNNCC	8 cut(s)	993, 994, 1102, 2012, 2299, 2334, 2614, 2706
BmrFI	CCNGG	3 cut(s)	1355, 1400, 1754
BmrI	ACTGGG	5 cut(s)	807, 915, 998, 2336, 3466
BmuI	ACTGGG	5 cut(s)	807, 915, 998, 2336, 3466
BpiI	GAAGAC	2 cut(s)	854, 3232
BpmI	CTGGAG	1 cut(s)	1119
Bpu1102I	GCTNAGC	1 cut(s)	323
BpuEI	CTTGAG	3 cut(s)	1145, 2574, 2666
BpuMI	CCSGG	1 cut(s)	1400
BsaAI	YACGTR	1 cut(s)	3004
BsaBI	GATNNNNATC	2 cut(s)	1367, 1980
BsaI	GGTCTC	2 cut(s)	1067, 1121
BsaJI	CCNNGG	5 cut(s)	225, 315, 556, 1831, 1930
BsaWI	WCCGGW	2 cut(s)	101, 3357
BsaXI	ACNNNNNCTCC	4 cut(s)	1041, 1071, 1594, 1624
Bsc4I	CCNNNNNNNGG	7 cut(s)	12, 541, 544, 1759, 1814, 2908, 2938
Bse118I	RCCGGY	1 cut(s)	1064
Bse3DI	GCAATG	3 cut(s)	2263, 2708, 2880
Bse8I	GATNNNNATC	2 cut(s)	1367, 1980
BseBI	CCWGG	2 cut(s)	1355, 1754
BseDI	CCNNGG	5 cut(s)	225, 315, 556, 1831, 1930
BseJI	GATNNNNATC	2 cut(s)	1367, 1980
BseLI	CCNNNNNNNGG	7 cut(s)	12, 541, 544, 1759, 1814, 2908, 2938
BseMI	GCAATG	3 cut(s)	2263, 2708, 2880
BseMII	CTCAG	5 cut(s)	337, 1284, 1536, 1717, 2165
BseRI	GAGGAG	5 cut(s)	318, 365, 1129, 2122, 2416
BseSI	GKGCMC	2 cut(s)	1045, 2015
BseYI	CCCAGC	2 cut(s)	142, 537
BsgI	GTGCAG	2 cut(s)	833, 3618
Bsh1236I	CGCG	1 cut(s)	3623
Bsh1285I	CGRYCG	2 cut(s)	1069, 3210
BshFI	GGCC	7 cut(s)	16, 555, 948, 1451, 1660, 2835, 3461
BshNI	GGYRCC	4 cut(s)	2010, 2297, 2332, 2612
BsiEI	CGRYCG	2 cut(s)	1069, 3210
BsiHKAI	GWGCWC	2 cut(s)	525, 3009
BsiSI	CCGG	5 cut(s)	102, 123, 1065, 1399, 3358
BslFI	GGGAC	5 cut(s)	316, 1005, 1825, 1949, 2405
BslI	CCNNNNNNNGG	7 cut(s)	12, 541, 544, 1759, 1814, 2908, 2938
BsmAI	GTCTC	5 cut(s)	1067, 1121, 1195, 1210, 3163
BsmBI	CGTCTC	1 cut(s)	1210
BsmFI	GGGAC	5 cut(s)	316, 1005, 1825, 1949, 2405
BsmI	GAATGC	3 cut(s)	956, 2977, 3182
BsnI	GGCC	7 cut(s)	16, 555, 948, 1451, 1660, 2835, 3461
Bso31I	GGTCTC	2 cut(s)	1067, 1121
Bsp1286I	GDGCHC	5 cut(s)	525, 1045, 1105, 2015, 3009
Bsp1407I	TGTACA	3 cut(s)	1181, 1377, 2625
Bsp1720I	GCTNAGC	1 cut(s)	323
BspACI	CCGC	9 cut(s)	13, 545, 631, 979, 1872, 1875, 2264, 2935, 3146
BspANI	GGCC	7 cut(s)	16, 555, 948, 1451, 1660, 2835, 3461
BspCNI	CTCAG	5 cut(s)	336, 1283, 1535, 1716, 2166
BspFNI	CGCG	1 cut(s)	3623
BspHI	TCATGA	2 cut(s)	1359, 1419
BspLI	GGNNCC	8 cut(s)	993, 994, 1102, 2012, 2299, 2334, 2614, 2706
BspMAI	CTGCAG	3 cut(s)	151, 2548, 3082
BspMI	ACCTGC	1 cut(s)	3672
BspPI	GGATC	9 cut(s)	19, 121, 283, 304, 785, 1357, 1799, 2794, 3400
BspQI	GCTCTTC	1 cut(s)	3014
BspT107I	GGYRCC	4 cut(s)	2010, 2297, 2332, 2612
BspTI	CTTAAG	1 cut(s)	1025
BspTNI	GGTCTC	2 cut(s)	1067, 1121
BsrDI	GCAATG	3 cut(s)	2263, 2708, 2880
BsrFI	RCCGGY	1 cut(s)	1064
BsrGI	TGTACA	3 cut(s)	1181, 1377, 2625
BssAI	RCCGGY	1 cut(s)	1064
BssECI	CCNNGG	5 cut(s)	225, 315, 556, 1831, 1930
BssT1I	CCWWGG	3 cut(s)	225, 1831, 1930
Bst2UI	CCWGG	2 cut(s)	1355, 1754
Bst4CI	ACNGT	9 cut(s)	480, 1186, 1598, 2026, 2471, 2539, 2639, 2678, 2918
Bst6I	CTCTTC	2 cut(s)	1982, 3014
BstAFI	CTTAAG	1 cut(s)	1025
BstAPI	GCANNNNNTGC	2 cut(s)	2225, 3482
BstAUI	TGTACA	3 cut(s)	1181, 1377, 2625
BstBAI	YACGTR	1 cut(s)	3004
BstC8I	GCNNGC	6 cut(s)	543, 1573, 2165, 2221, 2266, 2850
BstDSI	CCRYGG	1 cut(s)	556
BstEII	GGTNACC	1 cut(s)	683
BstFNI	CGCG	1 cut(s)	3623
BstH2I	RGCGCY	1 cut(s)	323
BstHHI	GCGC	2 cut(s)	322, 3623
BstMAI	GTCTC	5 cut(s)	1067, 1121, 1195, 1210, 3163
BstMCI	CGRYCG	2 cut(s)	1069, 3210
BstNI	CCWGG	2 cut(s)	1355, 1754
BstNSI	RCATGY	5 cut(s)	331, 1739, 2167, 3058, 3380
BstPI	GGTNACC	1 cut(s)	683
BstSCI	CCNGG	3 cut(s)	1353, 1398, 1752
BstSFI	CTRYAG	8 cut(s)	147, 625, 1252, 2181, 2544, 2965, 3078, 3565
BstSLI	GKGCMC	2 cut(s)	1045, 2015
BstUI	CGCG	1 cut(s)	3623
BstV2I	GAAGAC	2 cut(s)	854, 3232
BstX2I	RGATCY	2 cut(s)	3298, 3392
BstYI	RGATCY	2 cut(s)	3298, 3392
BsuRI	GGCC	7 cut(s)	16, 555, 948, 1451, 1660, 2835, 3461
BtgI	CCRYGG	1 cut(s)	556
BtgZI	GCGATG	1 cut(s)	282
BtrI	CACGTC	1 cut(s)	1800
BtsI	GCAGTG	5 cut(s)	2201, 2223, 2982, 3075, 3321
BveI	ACCTGC	1 cut(s)	3672
Cac8I	GCNNGC	6 cut(s)	543, 1573, 2165, 2221, 2266, 2850
CciI	TCATGA	2 cut(s)	1359, 1419
CfoI	GCGC	2 cut(s)	322, 3623
Cfr10I	RCCGGY	1 cut(s)	1064
Cfr13I	GGNCC	4 cut(s)	992, 3115, 3459, 3661
CseI	GACGC	2 cut(s)	1227, 3612
DraIII	CACNNNGTG	1 cut(s)	3222
EaeI	YGGCCR	2 cut(s)	553, 2833
Eam1104I	CTCTTC	2 cut(s)	1982, 3014
EarI	CTCTTC	2 cut(s)	1982, 3014
Eco130I	CCWWGG	3 cut(s)	225, 1831, 1930
Eco147I	AGGCCT	1 cut(s)	1451
Eco24I	GRGCYC	1 cut(s)	1105
Eco31I	GGTCTC	2 cut(s)	1067, 1121
Eco32I	GATATC	1 cut(s)	1927
Eco47I	GGWCC	3 cut(s)	992, 3115, 3661
Eco57I	CTGAAG	2 cut(s)	1676, 2358
Eco91I	GGTNACC	1 cut(s)	683
EcoO109I	RGGNCCY	2 cut(s)	992, 3115
EcoO65I	GGTNACC	1 cut(s)	683
EcoRI	GAATTC	2 cut(s)	130, 2186
EcoRII	CCWGG	2 cut(s)	1353, 1752
EcoRV	GATATC	1 cut(s)	1927
EcoT14I	CCWWGG	3 cut(s)	225, 1831, 1930
EcoT22I	ATGCAT	1 cut(s)	3382
EcoT38I	GRGCYC	1 cut(s)	1105
ErhI	CCWWGG	3 cut(s)	225, 1831, 1930
Esp3I	CGTCTC	1 cut(s)	1210
FalI	AAGNNNNNCTT	4 cut(s)	196, 228, 2212, 2244
FaqI	GGGAC	5 cut(s)	316, 1005, 1825, 1949, 2405
FauI	CCCGC	2 cut(s)	2257, 3153
FbaI	TGATCA	1 cut(s)	1422
FblI	GTMKAC	1 cut(s)	1794
FriOI	GRGCYC	1 cut(s)	1105
FspBI	CTAG	5 cut(s)	462, 2294, 2523, 2666, 3137
GlaI	GCGC	2 cut(s)	321, 3622
GsaI	CCCAGC	2 cut(s)	146, 541
GsuI	CTGGAG	1 cut(s)	1119
HaeII	RGCGCY	1 cut(s)	323
HaeIII	GGCC	7 cut(s)	16, 555, 948, 1451, 1660, 2835, 3461
HapII	CCGG	5 cut(s)	102, 123, 1065, 1399, 3358
HgaI	GACGC	2 cut(s)	1227, 3612
HhaI	GCGC	2 cut(s)	322, 3623
Hin6I	GCGC	2 cut(s)	320, 3621
HinP1I	GCGC	2 cut(s)	320, 3621
HincII	GTYRAC	1 cut(s)	3204
HindII	GTYRAC	1 cut(s)	3204
HindIII	AAGCTT	1 cut(s)	2432
HinfI	GANTC	8 cut(s)	311, 836, 1117, 1838, 2348, 2483, 2889, 3103
HpaI	GTTAAC	1 cut(s)	3204
HpaII	CCGG	5 cut(s)	102, 123, 1065, 1399, 3358
HphI	GGTGA	4 cut(s)	737, 922, 1684, 3224
Hpy166II	GTNNAC	8 cut(s)	1182, 1285, 1741, 1795, 2626, 2642, 2914, 3204
Hpy8I	GTNNAC	8 cut(s)	1182, 1285, 1741, 1795, 2626, 2642, 2914, 3204
Hpy99I	CGWCG	1 cut(s)	2564
HpyCH4III	ACNGT	9 cut(s)	480, 1186, 1598, 2026, 2471, 2539, 2639, 2678, 2918
HpyCH4IV	ACGT	5 cut(s)	168, 1328, 1799, 2790, 3003
HpySE526I	ACGT	5 cut(s)	168, 1328, 1799, 2790, 3003
HspAI	GCGC	2 cut(s)	320, 3621
KflI	GGGWCCC	1 cut(s)	992
Ksp22I	TGATCA	1 cut(s)	1422
KspAI	GTTAAC	1 cut(s)	3204
LguI	GCTCTTC	1 cut(s)	3014
MaeI	CTAG	5 cut(s)	462, 2294, 2523, 2666, 3137
MaeII	ACGT	5 cut(s)	168, 1328, 1799, 2790, 3003
MaeIII	GTNAC	8 cut(s)	595, 683, 882, 984, 1171, 2672, 3212, 3514
MfeI	CAATTG	1 cut(s)	3090
MflI	RGATCY	2 cut(s)	3298, 3392
MhlI	GDGCHC	5 cut(s)	525, 1045, 1105, 2015, 3009
MlsI	TGGCCA	1 cut(s)	2835
MluNI	TGGCCA	1 cut(s)	2835
MlyI	GAGTC	4 cut(s)	845, 1126, 1847, 2342
MmeI	TCCRAC	3 cut(s)	253, 818, 1215
Mox20I	TGGCCA	1 cut(s)	2835
Mph1103I	ATGCAT	1 cut(s)	3382
MroXI	GAANNNNTTC	3 cut(s)	208, 480, 1946
MscI	TGGCCA	1 cut(s)	2835
MslI	CAYNNNNRTG	3 cut(s)	1385, 2057, 2121
Msp20I	TGGCCA	1 cut(s)	2835
MspA1I	CMGCKG	5 cut(s)	146, 152, 541, 2935, 3521
MspCI	CTTAAG	1 cut(s)	1025
MspI	CCGG	5 cut(s)	102, 123, 1065, 1399, 3358
MspR9I	CCNGG	3 cut(s)	1355, 1400, 1754
MunI	CAATTG	1 cut(s)	3090
Mva1269I	GAATGC	3 cut(s)	956, 2977, 3182
MvaI	CCWGG	2 cut(s)	1355, 1754
MvnI	CGCG	1 cut(s)	3623
NciI	CCSGG	1 cut(s)	1400
NlaIV	GGNNCC	8 cut(s)	993, 994, 1102, 2012, 2299, 2334, 2614, 2706
NmeAIII	GCCGAG	2 cut(s)	1499, 2723
NmuCI	GTSAC	3 cut(s)	984, 3212, 3514
NsiI	ATGCAT	1 cut(s)	3382
NspI	RCATGY	5 cut(s)	331, 1739, 2167, 3058, 3380
PaeI	GCATGC	1 cut(s)	2167
PagI	TCATGA	2 cut(s)	1359, 1419
PceI	AGGCCT	1 cut(s)	1451
PciI	ACATGT	1 cut(s)	1735
PciSI	GCTCTTC	1 cut(s)	3014
PctI	GAATGC	3 cut(s)	956, 2977, 3182
PdmI	GAANNNNTTC	3 cut(s)	208, 480, 1946
PfeI	GAWTC	4 cut(s)	311, 2483, 2889, 3103
PflFI	GACNNNGTC	1 cut(s)	2539
PflMI	CCANNNNNTGG	1 cut(s)	2938
Ple19I	CGATCG	1 cut(s)	3210
PleI	GAGTC	4 cut(s)	844, 1125, 1846, 2342
PpsI	GAGTC	4 cut(s)	844, 1125, 1846, 2342
Ppu21I	YACGTR	1 cut(s)	3004
PpuMI	RGGWCCY	2 cut(s)	992, 3115
PscI	ACATGT	1 cut(s)	1735
PsiI	TTATAA	3 cut(s)	1293, 1593, 2462
Psp1406I	AACGTT	1 cut(s)	168
Psp5II	RGGWCCY	2 cut(s)	992, 3115
Psp6I	CCWGG	2 cut(s)	1353, 1752
PspEI	GGTNACC	1 cut(s)	683
PspFI	CCCAGC	2 cut(s)	142, 537
PspGI	CCWGG	2 cut(s)	1353, 1752
PspN4I	GGNNCC	8 cut(s)	993, 994, 1102, 2012, 2299, 2334, 2614, 2706
PspPI	GGNCC	4 cut(s)	992, 3115, 3459, 3661
PspPPI	RGGWCCY	2 cut(s)	992, 3115
PsrI	GAACNNNNNNTAC	2 cut(s)	31, 63
PstI	CTGCAG	3 cut(s)	151, 2548, 3082
PsuI	RGATCY	2 cut(s)	3298, 3392
PsyI	GACNNNGTC	1 cut(s)	2539
PvuI	CGATCG	1 cut(s)	3210
PvuII	CAGCTG	4 cut(s)	146, 152, 541, 3521
RseI	CAYNNNNRTG	3 cut(s)	1385, 2057, 2121
SapI	GCTCTTC	1 cut(s)	3014
Sau96I	GGNCC	4 cut(s)	992, 3115, 3459, 3661
ScaI	AGTACT	2 cut(s)	440, 3563
SchI	GAGTC	4 cut(s)	845, 1126, 1847, 2342
ScrFI	CCNGG	3 cut(s)	1355, 1400, 1754
SduI	GDGCHC	5 cut(s)	525, 1045, 1105, 2015, 3009
SfcI	CTRYAG	8 cut(s)	147, 625, 1252, 2181, 2544, 2965, 3078, 3565
SinI	GGWCC	3 cut(s)	992, 3115, 3661
SmiMI	CAYNNNNRTG	3 cut(s)	1385, 2057, 2121
SmlI	CTYRAG	4 cut(s)	1025, 1124, 2589, 2645
SmoI	CTYRAG	4 cut(s)	1025, 1124, 2589, 2645
SphI	GCATGC	1 cut(s)	2167
SseBI	AGGCCT	1 cut(s)	1451
SsiI	CCGC	9 cut(s)	13, 545, 631, 979, 1872, 1875, 2264, 2935, 3146
SspMI	CTAG	5 cut(s)	462, 2294, 2523, 2666, 3137
StuI	AGGCCT	1 cut(s)	1451
StyD4I	CCNGG	3 cut(s)	1353, 1398, 1752
StyI	CCWWGG	3 cut(s)	225, 1831, 1930
TaaI	ACNGT	9 cut(s)	480, 1186, 1598, 2026, 2471, 2539, 2639, 2678, 2918
TaiI	ACGT	5 cut(s)	171, 1331, 1802, 2793, 3006
TaqII	GACCGA	1 cut(s)	627
TatI	WGTACW	6 cut(s)	438, 1006, 1181, 1377, 2625, 3561
TauI	GCSGC	4 cut(s)	16, 634, 1874, 1877
TfiI	GAWTC	4 cut(s)	311, 2483, 2889, 3103
TseFI	GTSAC	3 cut(s)	984, 3212, 3514
Tsp45I	GTSAC	3 cut(s)	984, 3212, 3514
TspGWI	ACGGA	2 cut(s)	2577, 3171
Tth111I	GACNNNGTC	1 cut(s)	2539
Van91I	CCANNNNNTGG	1 cut(s)	2938
Vha464I	CTTAAG	1 cut(s)	1025
VpaK11BI	GGWCC	3 cut(s)	992, 3115, 3661
XapI	RAATTY	7 cut(s)	91, 130, 466, 841, 1559, 2186, 3596
XbaI	TCTAGA	1 cut(s)	3136
XceI	RCATGY	5 cut(s)	331, 1739, 2167, 3058, 3380
XcmI	CCANNNNNNNNNTGG	1 cut(s)	574
XmiI	GTMKAC	1 cut(s)	1794
XmnI	GAANNNNTTC	3 cut(s)	208, 480, 1946
XspI	CTAG	5 cut(s)	462, 2294, 2523, 2666, 3137
ZrmI	AGTACT	2 cut(s)	440, 3563
Zsp2I	ATGCAT	1 cut(s)	3382

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.