MD02G1300200.v1.1

Protein of unknown function (DUF2462)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014444
Physical Location & Seq
Forward (+)
35500837 .. 35502909
2073 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1300200.v1.1.491

Sequence Viewer

Length: 267 bp
ATGACGCAGAAAGGGAAGCTGTTCAAAGGGCAGCAGAAGAGGAAGACGATTCCTCCCAACCGCCATGGAAAAATGACTCAAATTCGCAAAGGCAAGCGAGTAATTAAGCCTTCCAAGGTCACCGGTGACATGGATTCCGATCGGGAGATAACAAAGTTCATAAACCACTGCAATGAGGTGAAGGCGGCCACCGCTGCAAACAAGGAAGGTGGCCAGTTAAGTATAGTCAAAACACCTCAGGACTCCGGTAACAATGCGAAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2462

Protein Analysis

89

Amino Acids

9.77

Weight (kDa)

10.43

Isoelectric Point (pI)

26.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2462 PF09495 1 - 80 1.2e-08 Protein of unknown function (DUF2462)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014444)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52040
fragaria_vesca FvH4_7g01820
malus_domestica MD02G1300200.v1.1 MD07G1024700.v1.1
prunus_persica Prupe.2G024400_v2.0.a1
pyrus_communis pycom02g25220
rosa_chinensis RchiOBHm_Chr1g0318451
rosa_multiflora Rmu_sc0004803.1_g000001
rosa_roxburghii Rroxscaffold_4G00329240
rosa_rugosa Rorug01G0021400
rosa_samantha Rh1AG033500 Rh1BG029500 Rh1CG031800 Rh1CG033200 Rh1DG037300 Rh1DG037700
rosa_wichuraiana Rw1G002680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 61, 185, 192
AcoI YGGCCR 2 cut(s) 186, 211
AcsI RAATTY 1 cut(s) 81
AgeI ACCGGT 1 cut(s) 122
AgsI TTSAA 1 cut(s) 25
AluBI AGCT 1 cut(s) 19
AluI AGCT 1 cut(s) 19
AoxI GGCC 2 cut(s) 186, 211
ApeKI GCWGC 2 cut(s) 31, 194
ApoI RAATTY 1 cut(s) 81
AsiGI ACCGGT 1 cut(s) 122
Asp700I GAANNNNTTC 1 cut(s) 20
AsuHPI GGTGA 3 cut(s) 112, 137, 190
AxyI CCTNAGG 1 cut(s) 237
BalI TGGCCA 1 cut(s) 213
BbsI GAAGAC 1 cut(s) 50
BbvI GCAGC 2 cut(s) 43, 181
BisI GCNGC 3 cut(s) 32, 186, 195
BlsI GCNGC 3 cut(s) 33, 187, 196
BpiI GAAGAC 1 cut(s) 50
BsaBI GATNNNNATC 1 cut(s) 138
BsaJI CCNNGG 2 cut(s) 64, 114
BsaWI WCCGGW 2 cut(s) 122, 245
BsaXI ACNNNNNCTCC 2 cut(s) 37, 67
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 1 cut(s) 214
Bse21I CCTNAGG 1 cut(s) 237
Bse3DI GCAATG 1 cut(s) 178
Bse8I GATNNNNATC 1 cut(s) 138
BseDI CCNNGG 2 cut(s) 64, 114
BseJI GATNNNNATC 1 cut(s) 138
BseMI GCAATG 1 cut(s) 178
BseMII CTCAG 1 cut(s) 251
BseNI ACTGG 1 cut(s) 214
BseXI GCAGC 2 cut(s) 43, 181
Bsh1285I CGRYCG 1 cut(s) 142
BshFI GGCC 2 cut(s) 188, 213
BshTI ACCGGT 1 cut(s) 122
BsiEI CGRYCG 1 cut(s) 142
BsiSI CCGG 2 cut(s) 123, 246
BsnI GGCC 2 cut(s) 188, 213
Bsp143I GATC 1 cut(s) 139
Bsp19I CCATGG 1 cut(s) 64
BspACI CCGC 3 cut(s) 61, 185, 192
BspANI GGCC 2 cut(s) 188, 213
BspCNI CTCAG 1 cut(s) 250
BsrDI GCAATG 1 cut(s) 178
BsrFI RCCGGY 1 cut(s) 122
BsrI ACTGG 1 cut(s) 214
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 2 cut(s) 64, 114
BssMI GATC 1 cut(s) 139
BssT1I CCWWGG 2 cut(s) 64, 114
Bst6I CTCTTC 1 cut(s) 32
BstC8I GCNNGC 1 cut(s) 95
BstDEI CTNAG 1 cut(s) 237
BstDSI CCRYGG 1 cut(s) 64
BstEII GGTNACC 1 cut(s) 118
BstKTI GATC 1 cut(s) 142
BstMBI GATC 1 cut(s) 139
BstMCI CGRYCG 1 cut(s) 142
BstMWI GCNNNNNNNGC 2 cut(s) 191, 194
BstPI GGTNACC 1 cut(s) 118
BstV1I GCAGC 2 cut(s) 43, 181
BstV2I GAAGAC 1 cut(s) 50
Bsu36I CCTNAGG 1 cut(s) 237
BsuRI GGCC 2 cut(s) 188, 213
BtgI CCRYGG 1 cut(s) 64
BtsI GCAGTG 1 cut(s) 166
BtsIMutI CAGTG 1 cut(s) 166
Cac8I GCNNGC 1 cut(s) 95
Cfr10I RCCGGY 1 cut(s) 122
CseI GACGC 1 cut(s) 13
CspAI ACCGGT 1 cut(s) 122
CspCI CAANNNNNGTGG 2 cut(s) 190, 225
CviAII CATG 2 cut(s) 65, 130
CviJI RGCY 4 cut(s) 19, 109, 188, 213
CviKI_1 RGCY 4 cut(s) 19, 109, 188, 213
DdeI CTNAG 1 cut(s) 237
DpnI GATC 1 cut(s) 141
DpnII GATC 1 cut(s) 139
EaeI YGGCCR 2 cut(s) 186, 211
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Eco130I CCWWGG 2 cut(s) 64, 114
Eco81I CCTNAGG 1 cut(s) 237
Eco91I GGTNACC 1 cut(s) 118
EcoO65I GGTNACC 1 cut(s) 118
EcoT14I CCWWGG 2 cut(s) 64, 114
ErhI CCWWGG 2 cut(s) 64, 114
FaeI CATG 2 cut(s) 68, 133
FaiI YATR 4 cut(s) 66, 131, 161, 224
FatI CATG 2 cut(s) 64, 129
Fnu4HI GCNGC 3 cut(s) 32, 186, 195
Fsp4HI GCNGC 3 cut(s) 32, 186, 195
GluI GCNGC 3 cut(s) 32, 186, 195
HaeIII GGCC 2 cut(s) 188, 213
HapII CCGG 2 cut(s) 123, 246
HgaI GACGC 1 cut(s) 13
Hin1II CATG 2 cut(s) 68, 133
HinfI GANTC 4 cut(s) 49, 76, 134, 242
HpaII CCGG 2 cut(s) 123, 246
HphI GGTGA 3 cut(s) 112, 137, 190
Hpy188I TCNGA 1 cut(s) 139
Hpy188III TCNNGA 2 cut(s) 143, 239
HpyAV CCTTC 3 cut(s) 120, 175, 200
HpyCH4V TGCA 2 cut(s) 171, 197
HpyF10VI GCNNNNNNNGC 2 cut(s) 191, 194
HpyF3I CTNAG 1 cut(s) 237
Hsp92II CATG 2 cut(s) 68, 133
Kzo9I GATC 1 cut(s) 139
LpnPI CCDG 4 cut(s) 136, 224, 227, 259
Lsp1109I GCAGC 2 cut(s) 43, 181
MaeIII GTNAC 3 cut(s) 118, 125, 248
MalI GATC 1 cut(s) 141
MboI GATC 1 cut(s) 139
MboII GAAGA 2 cut(s) 49, 55
MlsI TGGCCA 1 cut(s) 213
MluCI AATT 2 cut(s) 81, 102
MluNI TGGCCA 1 cut(s) 213
MlyI GAGTC 2 cut(s) 70, 236
MnlI CCTC 4 cut(s) 33, 63, 169, 246
Mox20I TGGCCA 1 cut(s) 213
MroXI GAANNNNTTC 1 cut(s) 20
MscI TGGCCA 1 cut(s) 213
MseI TTAA 2 cut(s) 105, 218
MslI CAYNNNNRTG 1 cut(s) 171
Msp20I TGGCCA 1 cut(s) 213
MspA1I CMGCKG 1 cut(s) 194
MspI CCGG 2 cut(s) 123, 246
MwoI GCNNNNNNNGC 2 cut(s) 191, 194
NcoI CCATGG 1 cut(s) 64
NdeII GATC 1 cut(s) 139
NlaIII CATG 2 cut(s) 68, 133
NmuCI GTSAC 2 cut(s) 118, 125
PdmI GAANNNNTTC 1 cut(s) 20
PfeI GAWTC 2 cut(s) 49, 134
PinAI ACCGGT 1 cut(s) 122
PkrI GCNGC 3 cut(s) 33, 187, 196
Ple19I CGATCG 1 cut(s) 142
PleI GAGTC 2 cut(s) 70, 236
PpsI GAGTC 2 cut(s) 70, 236
PspEI GGTNACC 1 cut(s) 118
PvuI CGATCG 1 cut(s) 142
RseI CAYNNNNRTG 1 cut(s) 171
SaqAI TTAA 2 cut(s) 105, 218
SatI GCNGC 3 cut(s) 32, 186, 195
Sau3AI GATC 1 cut(s) 139
SchI GAGTC 2 cut(s) 70, 236
SetI ASST 5 cut(s) 21, 120, 180, 211, 238
SgrAI CRCCGGYG 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 171
Sse9I AATT 2 cut(s) 81, 102
SsiI CCGC 3 cut(s) 61, 185, 192
StyI CCWWGG 2 cut(s) 64, 114
TasI AATT 2 cut(s) 81, 102
TauI GCSGC 1 cut(s) 188
TfiI GAWTC 2 cut(s) 49, 134
Tru1I TTAA 2 cut(s) 105, 218
Tru9I TTAA 2 cut(s) 105, 218
TscAI CASTG 1 cut(s) 173
TseFI GTSAC 2 cut(s) 118, 125
TseI GCWGC 2 cut(s) 31, 194
Tsp45I GTSAC 2 cut(s) 118, 125
TspDTI ATGAA 1 cut(s) 148
TspRI CASTG 1 cut(s) 173
XapI RAATTY 1 cut(s) 81
XmnI GAANNNNTTC 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.