Rorug01G0021400

Protein of unknown function (DUF2462)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0014444
Physical Location & Seq
Forward (+)
3481268 .. 3486394
5127 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0021400.1

Sequence Viewer

Length: 2697 bp
ATGGCTGCTCTGGCTCACGCCCTGCTCGGAATCATCCCAGCTCGGTGTGGTCTCTGCCGAGCCATTTATCCCAACCTCTGCTGCTCTTGGCGCCCTTCACCTAATCCCTCTGGATTCTCCATTTCCAGTAGGACCCACCTTGCTAATCTCACCGCCCGGTCCAGACTCAACGCCGCCCACACCCTCACCGTCCACAATGCTCCGGCTCCCCCCACTCTTGAAATCCCCGTCACCTGTTACCAGCTTATTGGCGTTCCTGATCTAGCTGAGAAAGATGAGGTTATTAAAGCGGTAATGGATTTGAAGAATTCTGAAGTTGAAGAGGGTTATACTGAGAATGCTATTAAATCTCGGCAGGACCTCTTAATGGATGTGAGAGATAAGCTTATTTTTGAACCAGAATATGCTGGTAATAGTCATGAAAATATCCGACCTAAATCTGCACTTCAAATCCCTTGGGCATGGTTGGCAGCTGCTCTTTGCCTCCTTCAAGAGGTTAGGGAAGTAGAGCTTGTGCTAGATATTGGTCAAGCAGCTATTAAGCATCCAGATGCTAAGCCATATATTCATGATATTCTTCTCTCTATGGCACTGGCTGAGTGTGCAACTGCAATGATTGGTTTTGAGAGCAATAAAGTGTCCCAGGGATTTGAAGCTATTTCTCGTGCTCAGAGTCTTCTAAGAAGTAAAAAATCTCTTGAGAAAATATCATTATTATCTAAGATTGACAGAGCGAAAACTATGTGTGAGTGTCTGATTGCATCAGAGCTTACTGATCTGAAACTAGAGGAAGCTCTTACGCTGTTTCTTCTCGGTAAGGATAATGAGGCTGCAGTTGTTGAAAAGCTGAAGAAGCTTGATGGAAGCAAAAACCAATTAATACAGTCTTTATGCAAACAAGGCGACCTCAAGCTAGCTCTACAACTTTTTCCTCACGAACCCAACCCCTCCCAACAGACCTGTGAGCTCTTAATCCTTTCTTGCATCCGCCGGGATTCTCTCTCTGATGGATTAGACGTGCATCGCCACCTTGTTGATGGCGGGTGGGACCAAGACCCTTTTTTGGCCACCAAACTTATAGAGATGTACTCTGAGTTGGACTCCATCGAGAATGCACGTGAAGTATTCGATAAAACTCGTAAAAGAACTATTTATATGTGGAATGCACTTTTTAGAGCCCTTACGTTGGCTGGCCATGGAACTGAGGTGCTAAATTTGTACAGACAAATGAATACAATTGGGGTTTCGTCTGATCGGTTTACGTATACGTATGTACTCAAAGCTTGTGTTGTTTCAGAGATTTTGAGTTCGCTTCTCCAGAAGGGAAAGGAGATTCATGGGCATATTTTGCGTCACGGATATGGGGTCCATGTCCATGTAACGACCACTTTGTTGGATATGTATGCAAGGTTCGGGTGCGTTTCATGTGCTAGTAGTGTATTTGATCAGATGCAAAATAGAAATGTGGTTTCTTGGAGTGCTATGATTGCTTGCTATGCAAAGAATGCAAGGCCTTATGAAGCTTTGGAACTTTTTCGGAAGATGATACTAGAGGCCCCTGATTTGTTTCCCAACTCCGTGACCATGGTTAGTGTTCTTCAAGCTTGTGCAGCTCTTGCTGCCTTGGAGCAGGGGAAGTTCATTCATGGGTACATCCTGAGAAGGGGTCTTGATTCAACCCTTCCGGTTATGAGTAGCCTTATCACAATGTATGCGAGATGTGGTAAGCTTGAGCTGGGCCAACAAGTTTTCAATATGATAAAAAAGAAGGATGTGGTATCATGGAATTCCTTGATTTCAAGTTATGGAATTCATGGATATGGAAAGAGGGCAATTCAAATTTTTGAAGACATGATCAATCATGGAGTATCACCGAGTCATATTTCATTTGTTAGTGTTTTGGGGGCCTGTAGTCATGAAGGTCTTGTTGACGAGGGGAAGTTAATATTCCAGTCTATGGTGAGAGATCATGGATTATATCCCAGCATTGAGCAGTATGCTTGTATGGTGGATCTTCTTGGTCGTGCTAATCGGTTAGATGAAGCAGCCAAGGTCATTGAAGATATGCGGATTGAACCAGGAGCTCAAGTTTGGGGTGCTCTTCTTGGATCATGTAGGATACATGGTAATGTTGAGCTGGCAGAGAGAGCAAGCAAAAGGTTATTTGAGCTGGAGCCTAGGAATGCTGGGAATTATGTACTTCTAGCAGATATTTATGCTGAGGCCAAGATGTGGGATGAGGTAAAAAGAGTAAAAAAACTTCTAGAAGCTCGGGAACTTCAAAAGGTACCAGGTCGCAGTTGGATTGAAGTTAATAGGAAGATATACTCATTCGATTCCGTTGACGAGTTTAACCCACAAATAGAGCAACTTCATGCACTGCTGGGTGAATTGTCAATAGAGATGAAAGGACGAGGATATAAGCCACAGACTAAAGTTGTCCTCTATGATCTTGATGAGGAAGAGAAGGAACGTATAGTATTAGGCCATAGTGAAAAGTTAGCAGTTGCATTTGGTCTCATCAATACCAAAAATGGGGAAACTATTCGGATTTCCAAGAATTTGAGGCTATGTGAAGATTGCCATTATGTTACAAAGTTCATATCCAAGTTTACCAATAGAGAGATTTTAGTCCGAGATGTAAATCGGTTTCACCATTTCCGTGACGGGGTTTGTTCATGCGGGGATTATTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2462

Protein Analysis

898

Amino Acids

100.81

Weight (kDa)

6.74

Isoelectric Point (pI)

46.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arm_PDR PF25515 150 - 243 2e-23 Plastid division regulator ARM repeat
PPR_2 PF13041 383 - 429 4.9e-06 PPR repeat family
PPR_2 PF13041 487 - 537 5.3e-06 PPR repeat family
PPR PF01535 490 - 516 5.6e-06 PPR repeat
PPR_2 PF13041 590 - 638 1.2e-09 PPR repeat family
PPR PF01535 593 - 623 1e-06 PPR repeat
E_motif PF20431 708 - 770 3.1e-20 E motif
DYW_deaminase PF14432 806 - 898 1.2e-42 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014444)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52040
fragaria_vesca FvH4_7g01820
malus_domestica MD02G1300200.v1.1 MD07G1024700.v1.1
prunus_persica Prupe.2G024400_v2.0.a1
pyrus_communis pycom02g25220
rosa_chinensis RchiOBHm_Chr1g0318451
rosa_multiflora Rmu_sc0004803.1_g000001
rosa_roxburghii Rroxscaffold_4G00329240
rosa_rugosa Rorug01G0021400
rosa_samantha Rh1AG033500 Rh1BG029500 Rh1CG031800 Rh1CG033200 Rh1DG037300 Rh1DG037700
rosa_wichuraiana Rw1G002680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 2287
AccB1I GGYRCC 2 cut(s) 90, 2287
AccB7I CCANNNNNTGG 2 cut(s) 1957, 2232
AccI GTMKAC 1 cut(s) 1265
AciI CCGC 7 cut(s) 153, 174, 290, 988, 1041, 2068, 2682
AclWI GGATC 2 cut(s) 2019, 2116
AcoI YGGCCR 2 cut(s) 1065, 1192
AcsI RAATTY 6 cut(s) 307, 1213, 1786, 1809, 1839, 2560
AcuI CTGAAG 2 cut(s) 333, 869
AcvI CACGTG 1 cut(s) 1118
AcyI GRCGYC 1 cut(s) 91
AfaI GTAC 6 cut(s) 1088, 1220, 1275, 1652, 2199, 2289
AfiI CCNNNNNNNGG 9 cut(s) 367, 493, 1063, 1186, 1663, 1957, 2232, 2535, 2668
AjiI CACGTC 1 cut(s) 1018
AjnI CCWGG 3 cut(s) 642, 2077, 2290
Alw21I GWGCWC 4 cut(s) 670, 969, 2086, 2101
Alw26I GTCTC 2 cut(s) 56, 2522
AlwI GGATC 2 cut(s) 2019, 2116
Ama87I CYCGRG 1 cut(s) 2271
AoxI GGCC 8 cut(s) 1065, 1192, 1511, 1554, 1738, 1905, 2223, 2485
ApeKI GCWGC 9 cut(s) 5, 81, 470, 473, 533, 830, 1610, 1619, 2045
ApoI RAATTY 6 cut(s) 307, 1213, 1786, 1809, 1839, 2560
AseI ATTAAT 1 cut(s) 878
Asp700I GAANNNNTTC 2 cut(s) 1533, 2544
Asp718I GGTACC 1 cut(s) 2287
AspA2I CCTAGG 1 cut(s) 2177
AspLEI GCGC 1 cut(s) 93
AspS9I GGNCC 8 cut(s) 132, 159, 358, 1048, 1366, 1555, 1738, 1905
AsuC2I CCSGG 2 cut(s) 157, 992
AsuHPI GGTGA 8 cut(s) 90, 142, 178, 223, 1863, 1972, 2399, 2645
AsuNHI GCTAGC 1 cut(s) 913
AvaI CYCGRG 1 cut(s) 2271
AvaII GGWCC 5 cut(s) 132, 159, 358, 1048, 1366
AvrII CCTAGG 1 cut(s) 2177
BalI TGGCCA 2 cut(s) 1067, 1194
BanI GGYRCC 2 cut(s) 90, 2287
BanII GRGCYC 3 cut(s) 969, 1180, 2086
BarI GAAGNNNNNNTAC 2 cut(s) 2244, 2276
BauI CACGAG 1 cut(s) 663
BbrPI CACGTG 1 cut(s) 1118
BbsI GAAGAC 2 cut(s) 668, 1854
Bbv12I GWGCWC 4 cut(s) 670, 969, 2086, 2101
BbvCI CCTCAGC 1 cut(s) 2220
BbvI GCAGC 8 cut(s) 68, 460, 482, 545, 817, 1606, 1622, 2057
BccI CCATC 4 cut(s) 854, 1001, 1031, 1112
BciT130I CCWGG 3 cut(s) 644, 2079, 2292
BciVI GTATCC 1 cut(s) 2112
BclI TGATCA 2 cut(s) 1444, 1854
BcnI CCSGG 2 cut(s) 157, 992
BcoDI GTCTC 2 cut(s) 56, 2522
BfaI CTAG 9 cut(s) 263, 518, 785, 914, 1431, 1550, 2178, 2204, 2264
BfmI CTRYAG 2 cut(s) 831, 1909
BfoI RGCGCY 1 cut(s) 94
BfuI GTATCC 1 cut(s) 2112
BlnI CCTAGG 1 cut(s) 2177
BlpI GCTNAGC 1 cut(s) 555
Bme1390I CCNGG 5 cut(s) 157, 644, 992, 2079, 2292
Bme18I GGWCC 5 cut(s) 132, 159, 358, 1048, 1366
BmeT110I CYCGRG 1 cut(s) 2271
BmgBI CACGTC 1 cut(s) 1018
BmgT120I GGNCC 8 cut(s) 132, 159, 358, 1048, 1366, 1555, 1738, 1905
BmiI GGNNCC 9 cut(s) 92, 134, 207, 1049, 1367, 1557, 1906, 2175, 2289
BmrFI CCNGG 5 cut(s) 157, 644, 992, 2079, 2292
BmsI GCATC 6 cut(s) 541, 553, 770, 993, 1030, 1440
BmtI GCTAGC 1 cut(s) 917
BpiI GAAGAC 2 cut(s) 668, 1854
BplI GAGNNNNNCTC 4 cut(s) 1073, 1105, 1085, 1117
BpmI CTGGAG 2 cut(s) 1301, 2192
Bpu10I CCTNAGC 1 cut(s) 2220
Bpu1102I GCTNAGC 1 cut(s) 555
BpuEI CTTGAG 4 cut(s) 719, 893, 1751, 2070
BpuMI CCSGG 2 cut(s) 157, 992
BsaAI YACGTR 3 cut(s) 1118, 1263, 1269
BsaBI GATNNNNATC 1 cut(s) 2643
BsaHI GRCGYC 1 cut(s) 91
BsaI GGTCTC 2 cut(s) 56, 2522
BsaJI CCNNGG 8 cut(s) 455, 642, 643, 1195, 1584, 1623, 2049, 2177
BsaWI WCCGGW 1 cut(s) 1684
BsaXI ACNNNNNCTCC 2 cut(s) 2073, 2103
Bsc4I CCNNNNNNNGG 9 cut(s) 367, 493, 1063, 1186, 1663, 1957, 2232, 2535, 2668
Bse1I ACTGG 3 cut(s) 126, 597, 1951
Bse3DI GCAATG 1 cut(s) 618
Bse8I GATNNNNATC 1 cut(s) 2643
BseBI CCWGG 3 cut(s) 644, 2079, 2292
BseDI CCNNGG 8 cut(s) 455, 642, 643, 1195, 1584, 1623, 2049, 2177
BseGI GGATG 7 cut(s) 33, 376, 544, 984, 1653, 1777, 2242
BseJI GATNNNNATC 1 cut(s) 2643
BseLI CCNNNNNNNGG 9 cut(s) 367, 493, 1063, 1186, 1663, 1957, 2232, 2535, 2668
BseMI GCAATG 1 cut(s) 618
BseMII CTCAG 8 cut(s) 258, 324, 588, 683, 1083, 1194, 1649, 2211
BseNI ACTGG 3 cut(s) 126, 597, 1951
BseXI GCAGC 8 cut(s) 68, 460, 482, 545, 817, 1606, 1622, 2057
BseYI CCCAGC 5 cut(s) 37, 1735, 1982, 2186, 2383
BsgI GTGCAG 2 cut(s) 426, 1629
BshFI GGCC 8 cut(s) 1067, 1194, 1513, 1556, 1740, 1907, 2225, 2487
BshNI GGYRCC 2 cut(s) 90, 2287
BsiHKAI GWGCWC 4 cut(s) 670, 969, 2086, 2101
BsiHKCI CYCGRG 1 cut(s) 2271
BsiSI CCGG 4 cut(s) 157, 203, 991, 1685
BslFI GGGAC 2 cut(s) 625, 1061
BslI CCNNNNNNNGG 9 cut(s) 367, 493, 1063, 1186, 1663, 1957, 2232, 2535, 2668
BsmAI GTCTC 2 cut(s) 56, 2522
BsmFI GGGAC 2 cut(s) 625, 1061
BsmI GAATGC 5 cut(s) 343, 1117, 1168, 1510, 2188
BsnI GGCC 8 cut(s) 1067, 1194, 1513, 1556, 1740, 1907, 2225, 2487
Bso31I GGTCTC 2 cut(s) 56, 2522
BsoBI CYCGRG 1 cut(s) 2271
Bsp1286I GDGCHC 5 cut(s) 670, 969, 1180, 2086, 2101
Bsp1407I TGTACA 1 cut(s) 1218
Bsp143I GATC 9 cut(s) 259, 775, 1252, 1444, 1854, 1966, 2011, 2108, 2449
Bsp1720I GCTNAGC 1 cut(s) 555
Bsp19I CCATGG 2 cut(s) 1195, 1584
BspACI CCGC 7 cut(s) 153, 174, 290, 988, 1041, 2068, 2682
BspANI GGCC 8 cut(s) 1067, 1194, 1513, 1556, 1740, 1907, 2225, 2487
BspCNI CTCAG 8 cut(s) 259, 325, 589, 682, 1084, 1195, 1650, 2212
BspHI TCATGA 3 cut(s) 418, 568, 1915
BspLI GGNNCC 9 cut(s) 92, 134, 207, 1049, 1367, 1557, 1906, 2175, 2289
BspMAI CTGCAG 1 cut(s) 835
BspOI GCTAGC 1 cut(s) 917
BspPI GGATC 2 cut(s) 2019, 2116
BspQI GCTCTTC 1 cut(s) 2106
BspT107I GGYRCC 2 cut(s) 90, 2287
BspTNI GGTCTC 2 cut(s) 56, 2522
BsrDI GCAATG 1 cut(s) 618
BsrGI TGTACA 1 cut(s) 1218
BsrI ACTGG 3 cut(s) 126, 597, 1951
BssECI CCNNGG 8 cut(s) 455, 642, 643, 1195, 1584, 1623, 2049, 2177
BssMI GATC 9 cut(s) 259, 775, 1252, 1444, 1854, 1966, 2011, 2108, 2449
BssNAI GTATAC 1 cut(s) 1266
BssNI GRCGYC 1 cut(s) 91
BssSI CACGAG 1 cut(s) 663
BssT1I CCWWGG 6 cut(s) 455, 1195, 1584, 1623, 2049, 2177
Bst1107I GTATAC 1 cut(s) 1266
Bst2BI CACGAG 1 cut(s) 663
Bst2UI CCWGG 3 cut(s) 644, 2079, 2292
Bst4CI ACNGT 2 cut(s) 190, 885
Bst6I CTCTTC 3 cut(s) 315, 2106, 2457
BstACI GRCGYC 1 cut(s) 91
BstAPI GCANNNNNTGC 3 cut(s) 1348, 1505, 1616
BstAUI TGTACA 1 cut(s) 1218
BstBAI YACGTR 3 cut(s) 1118, 1263, 1269
BstC8I GCNNGC 5 cut(s) 915, 1192, 1492, 2139, 2152
BstDSI CCRYGG 2 cut(s) 1195, 1584
BstENI CCTNNNNNAGG 1 cut(s) 491
BstF5I GGATG 7 cut(s) 33, 376, 544, 984, 1653, 1777, 2242
BstH2I RGCGCY 1 cut(s) 94
BstHHI GCGC 1 cut(s) 93
BstKTI GATC 9 cut(s) 262, 778, 1255, 1447, 1857, 1969, 2014, 2111, 2452
BstMAI GTCTC 2 cut(s) 56, 2522
BstMBI GATC 9 cut(s) 259, 775, 1252, 1444, 1854, 1966, 2011, 2108, 2449
BstNI CCWGG 3 cut(s) 644, 2079, 2292
BstSCI CCNGG 5 cut(s) 155, 642, 990, 2077, 2290
BstSFI CTRYAG 2 cut(s) 831, 1909
BstSNI TACGTA 2 cut(s) 1263, 1269
BstV1I GCAGC 8 cut(s) 68, 460, 482, 545, 817, 1606, 1622, 2057
BstV2I GAAGAC 2 cut(s) 668, 1854
BstX2I RGATCY 1 cut(s) 2011
BstXI CCANNNNNNTGG 2 cut(s) 248, 1393
BstYI RGATCY 1 cut(s) 2011
BstZ17I GTATAC 1 cut(s) 1266
BsuI GTATCC 1 cut(s) 2112
BsuRI GGCC 8 cut(s) 1067, 1194, 1513, 1556, 1740, 1907, 2225, 2487
BtgI CCRYGG 2 cut(s) 1195, 1584
BtgZI GCGATG 1 cut(s) 1007
BtrI CACGTC 1 cut(s) 1018
BtsCI GGATG 7 cut(s) 33, 376, 544, 984, 1653, 1777, 2242
BtsI GCAGTG 1 cut(s) 2378
BtsIMutI CAGTG 2 cut(s) 590, 2378
Cac8I GCNNGC 5 cut(s) 915, 1192, 1492, 2139, 2152
CciI TCATGA 3 cut(s) 418, 568, 1915
CfoI GCGC 1 cut(s) 93
Cfr13I GGNCC 8 cut(s) 132, 159, 358, 1048, 1366, 1555, 1738, 1905
CseI GACGC 1 cut(s) 1340
CsiI ACCWGGT 1 cut(s) 2290
Csp6I GTAC 6 cut(s) 1087, 1219, 1274, 1651, 2198, 2288
CviQI GTAC 6 cut(s) 1087, 1219, 1274, 1651, 2198, 2288
DinI GGCGCC 1 cut(s) 92
DpnI GATC 9 cut(s) 261, 777, 1254, 1446, 1856, 1968, 2013, 2110, 2451
DpnII GATC 9 cut(s) 259, 775, 1252, 1444, 1854, 1966, 2011, 2108, 2449
EaeI YGGCCR 2 cut(s) 1065, 1192
Eam1104I CTCTTC 3 cut(s) 315, 2106, 2457
EarI CTCTTC 3 cut(s) 315, 2106, 2457
EciI GGCGGA 1 cut(s) 977
Ecl136II GAGCTC 2 cut(s) 967, 2084
Eco105I TACGTA 2 cut(s) 1263, 1269
Eco130I CCWWGG 6 cut(s) 455, 1195, 1584, 1623, 2049, 2177
Eco147I AGGCCT 1 cut(s) 1513
Eco24I GRGCYC 3 cut(s) 969, 1180, 2086
Eco31I GGTCTC 2 cut(s) 56, 2522
Eco47I GGWCC 5 cut(s) 132, 159, 358, 1048, 1366
Eco53kI GAGCTC 2 cut(s) 967, 2084
Eco57I CTGAAG 2 cut(s) 333, 869
Eco72I CACGTG 1 cut(s) 1118
Eco88I CYCGRG 1 cut(s) 2271
EcoICRI GAGCTC 2 cut(s) 967, 2084
EcoNI CCTNNNNNAGG 1 cut(s) 491
EcoO109I RGGNCCY 4 cut(s) 132, 358, 1555, 1905
EcoRI GAATTC 3 cut(s) 307, 1786, 1809
EcoRII CCWGG 3 cut(s) 642, 2077, 2290
EcoT14I CCWWGG 6 cut(s) 455, 1195, 1584, 1623, 2049, 2177
EcoT38I GRGCYC 3 cut(s) 969, 1180, 2086
EgeI GGCGCC 1 cut(s) 92
EheI GGCGCC 1 cut(s) 92
ErhI CCWWGG 6 cut(s) 455, 1195, 1584, 1623, 2049, 2177
FalI AAGNNNNNCTT 2 cut(s) 495, 527
FaqI GGGAC 2 cut(s) 625, 1061
FauI CCCGC 2 cut(s) 1034, 2675
FbaI TGATCA 2 cut(s) 1444, 1854
FblI GTMKAC 1 cut(s) 1265
FokI GGATG 7 cut(s) 20, 383, 531, 971, 1640, 1784, 2249
FriOI GRGCYC 3 cut(s) 969, 1180, 2086
FspBI CTAG 9 cut(s) 263, 518, 785, 914, 1431, 1550, 2178, 2204, 2264
GlaI GCGC 1 cut(s) 92
GsaI CCCAGC 5 cut(s) 41, 1739, 1986, 2190, 2387
GsuI CTGGAG 2 cut(s) 1301, 2192
HaeII RGCGCY 1 cut(s) 94
HaeIII GGCC 8 cut(s) 1067, 1194, 1513, 1556, 1740, 1907, 2225, 2487
HapII CCGG 4 cut(s) 157, 203, 991, 1685
HgaI GACGC 1 cut(s) 1340
HhaI GCGC 1 cut(s) 93
Hin1I GRCGYC 1 cut(s) 91
Hin6I GCGC 1 cut(s) 91
HinP1I GCGC 1 cut(s) 91
HincII GTYRAC 2 cut(s) 1930, 2344
HindII GTYRAC 2 cut(s) 1930, 2344
HindIII AAGCTT 6 cut(s) 383, 854, 1281, 1521, 1602, 1727
HpaII CCGG 4 cut(s) 157, 203, 991, 1685
HphI GGTGA 8 cut(s) 90, 142, 178, 223, 1863, 1972, 2399, 2645
Hpy166II GTNNAC 6 cut(s) 193, 1260, 1266, 1930, 2344, 2613
Hpy8I GTNNAC 6 cut(s) 193, 1260, 1266, 1930, 2344, 2613
HpyAV CCTTC 8 cut(s) 105, 497, 1315, 1656, 1691, 1762, 1913, 2461
HpyCH4III ACNGT 2 cut(s) 190, 885
HpyCH4IV ACGT 6 cut(s) 1017, 1117, 1184, 1262, 1268, 2473
HpySE526I ACGT 6 cut(s) 1017, 1117, 1184, 1262, 1268, 2473
Hsp92I GRCGYC 1 cut(s) 91
HspAI GCGC 1 cut(s) 91
KasI GGCGCC 1 cut(s) 90
KpnI GGTACC 1 cut(s) 2291
Ksp22I TGATCA 2 cut(s) 1444, 1854
Kzo9I GATC 9 cut(s) 259, 775, 1252, 1444, 1854, 1966, 2011, 2108, 2449
LguI GCTCTTC 1 cut(s) 2106
LmnI GCTCC 5 cut(s) 205, 211, 1627, 2081, 2173
Lsp1109I GCAGC 8 cut(s) 68, 460, 482, 545, 817, 1606, 1622, 2057
LweI GCATC 6 cut(s) 541, 553, 770, 993, 1030, 1440
MabI ACCWGGT 1 cut(s) 2290
MaeI CTAG 9 cut(s) 263, 518, 785, 914, 1431, 1550, 2178, 2204, 2264
MaeII ACGT 6 cut(s) 1017, 1117, 1184, 1262, 1268, 2473
MaeIII GTNAC 7 cut(s) 229, 236, 1352, 1378, 1579, 2590, 2663
MalI GATC 9 cut(s) 261, 777, 1254, 1446, 1856, 1968, 2013, 2110, 2451
MboI GATC 9 cut(s) 259, 775, 1252, 1444, 1854, 1966, 2011, 2108, 2449
MfeI CAATTG 1 cut(s) 1236
MflI RGATCY 1 cut(s) 2011
MhlI GDGCHC 5 cut(s) 670, 969, 1180, 2086, 2101
MlsI TGGCCA 2 cut(s) 1067, 1194
MluNI TGGCCA 2 cut(s) 1067, 1194
Mly113I GGCGCC 1 cut(s) 91
MlyI GAGTC 4 cut(s) 159, 682, 1094, 1885
MmeI TCCRAC 4 cut(s) 454, 1077, 1374, 2282
Mox20I TGGCCA 2 cut(s) 1067, 1194
MroXI GAANNNNTTC 2 cut(s) 1533, 2544
MscI TGGCCA 2 cut(s) 1067, 1194
MseI TTAA 9 cut(s) 285, 345, 365, 540, 878, 971, 1943, 2313, 2352
MslI CAYNNNNRTG 6 cut(s) 549, 1359, 1374, 1818, 2127, 2661
Msp20I TGGCCA 2 cut(s) 1067, 1194
MspA1I CMGCKG 1 cut(s) 473
MspI CCGG 4 cut(s) 157, 203, 991, 1685
MspR9I CCNGG 5 cut(s) 157, 644, 992, 2079, 2292
MunI CAATTG 1 cut(s) 1236
Mva1269I GAATGC 5 cut(s) 343, 1117, 1168, 1510, 2188
MvaI CCWGG 3 cut(s) 644, 2079, 2292
NarI GGCGCC 1 cut(s) 91
NciI CCSGG 2 cut(s) 157, 992
NcoI CCATGG 2 cut(s) 1195, 1584
NdeII GATC 9 cut(s) 259, 775, 1252, 1444, 1854, 1966, 2011, 2108, 2449
NheI GCTAGC 1 cut(s) 913
NlaIV GGNNCC 9 cut(s) 92, 134, 207, 1049, 1367, 1557, 1906, 2175, 2289
NmeAIII GCCGAG 2 cut(s) 83, 331
NmuCI GTSAC 4 cut(s) 229, 1352, 1579, 2663
PagI TCATGA 3 cut(s) 418, 568, 1915
PasI CCCWGGG 1 cut(s) 643
PceI AGGCCT 1 cut(s) 1513
PciSI GCTCTTC 1 cut(s) 2106
PctI GAATGC 5 cut(s) 343, 1117, 1168, 1510, 2188
PdmI GAANNNNTTC 2 cut(s) 1533, 2544
PfeI GAWTC 6 cut(s) 30, 114, 995, 1333, 1673, 2336
PflMI CCANNNNNTGG 2 cut(s) 1957, 2232
PleI GAGTC 4 cut(s) 159, 681, 1094, 1884
PluTI GGCGCC 1 cut(s) 94
PmaCI CACGTG 1 cut(s) 1118
PmlI CACGTG 1 cut(s) 1118
PpsI GAGTC 4 cut(s) 159, 681, 1094, 1884
Ppu21I YACGTR 3 cut(s) 1118, 1263, 1269
PpuMI RGGWCCY 2 cut(s) 132, 358
PshBI ATTAAT 1 cut(s) 878
Psp124BI GAGCTC 2 cut(s) 969, 2086
Psp5II RGGWCCY 2 cut(s) 132, 358
Psp6I CCWGG 3 cut(s) 642, 2077, 2290
PspCI CACGTG 1 cut(s) 1118
PspFI CCCAGC 5 cut(s) 37, 1735, 1982, 2186, 2383
PspGI CCWGG 3 cut(s) 642, 2077, 2290
PspN4I GGNNCC 9 cut(s) 92, 134, 207, 1049, 1367, 1557, 1906, 2175, 2289
PspPI GGNCC 8 cut(s) 132, 159, 358, 1048, 1366, 1555, 1738, 1905
PspPPI RGGWCCY 2 cut(s) 132, 358
PsrI GAACNNNNNNTAC 2 cut(s) 1394, 1426
PstI CTGCAG 1 cut(s) 835
PsuI RGATCY 1 cut(s) 2011
PvuII CAGCTG 1 cut(s) 473
RsaI GTAC 6 cut(s) 1088, 1220, 1275, 1652, 2199, 2289
RsaNI GTAC 6 cut(s) 1087, 1219, 1274, 1651, 2198, 2288
RseI CAYNNNNRTG 6 cut(s) 549, 1359, 1374, 1818, 2127, 2661
SacI GAGCTC 2 cut(s) 969, 2086
SapI GCTCTTC 1 cut(s) 2106
SaqAI TTAA 9 cut(s) 285, 345, 365, 540, 878, 971, 1943, 2313, 2352
Sau3AI GATC 9 cut(s) 259, 775, 1252, 1444, 1854, 1966, 2011, 2108, 2449
Sau96I GGNCC 8 cut(s) 132, 159, 358, 1048, 1366, 1555, 1738, 1905
SchI GAGTC 4 cut(s) 159, 682, 1094, 1885
ScrFI CCNGG 5 cut(s) 157, 644, 992, 2079, 2292
SduI GDGCHC 5 cut(s) 670, 969, 1180, 2086, 2101
SexAI ACCWGGT 1 cut(s) 2290
SfaNI GCATC 6 cut(s) 541, 553, 770, 993, 1030, 1440
SfcI CTRYAG 2 cut(s) 831, 1909
SfoI GGCGCC 1 cut(s) 92
SinI GGWCC 5 cut(s) 132, 159, 358, 1048, 1366
SmiMI CAYNNNNRTG 6 cut(s) 549, 1359, 1374, 1818, 2127, 2661
SmlI CTYRAG 4 cut(s) 698, 908, 1730, 2085
SmoI CTYRAG 4 cut(s) 698, 908, 1730, 2085
SnaBI TACGTA 2 cut(s) 1263, 1269
SseBI AGGCCT 1 cut(s) 1513
SsiI CCGC 7 cut(s) 153, 174, 290, 988, 1041, 2068, 2682
SspDI GGCGCC 1 cut(s) 90
SspI AATATT 1 cut(s) 1947
SspMI CTAG 9 cut(s) 263, 518, 785, 914, 1431, 1550, 2178, 2204, 2264
SstI GAGCTC 2 cut(s) 969, 2086
StuI AGGCCT 1 cut(s) 1513
StyD4I CCNGG 5 cut(s) 155, 642, 990, 2077, 2290
StyI CCWWGG 6 cut(s) 455, 1195, 1584, 1623, 2049, 2177
TaaI ACNGT 2 cut(s) 190, 885
TaiI ACGT 6 cut(s) 1020, 1120, 1187, 1265, 1271, 2476
TaqI TCGA 3 cut(s) 1107, 1128, 2334
TatI WGTACW 4 cut(s) 1086, 1218, 1273, 2197
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 6 cut(s) 30, 114, 995, 1333, 1673, 2336
Tru1I TTAA 9 cut(s) 285, 345, 365, 540, 878, 971, 1943, 2313, 2352
Tru9I TTAA 9 cut(s) 285, 345, 365, 540, 878, 971, 1943, 2313, 2352
TscAI CASTG 2 cut(s) 597, 2385
TseFI GTSAC 4 cut(s) 229, 1352, 1579, 2663
TseI GCWGC 9 cut(s) 5, 81, 470, 473, 533, 830, 1610, 1619, 2045
Tsp45I GTSAC 4 cut(s) 229, 1352, 1579, 2663
TspGWI ACGGA 4 cut(s) 1371, 1567, 2329, 2651
TspRI CASTG 2 cut(s) 597, 2385
Van91I CCANNNNNTGG 2 cut(s) 1957, 2232
VpaK11BI GGWCC 5 cut(s) 132, 159, 358, 1048, 1366
VspI ATTAAT 1 cut(s) 878
XagI CCTNNNNNAGG 1 cut(s) 491
XapI RAATTY 6 cut(s) 307, 1213, 1786, 1809, 1839, 2560
XbaI TCTAGA 1 cut(s) 2263
XcmI CCANNNNNNNNNTGG 2 cut(s) 1034, 2298
XmaJI CCTAGG 1 cut(s) 2177
XmiI GTMKAC 1 cut(s) 1265
XmnI GAANNNNTTC 2 cut(s) 1533, 2544
XspI CTAG 9 cut(s) 263, 518, 785, 914, 1431, 1550, 2178, 2204, 2264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.