MD07G1024700.v1.1

Protein of unknown function (DUF2462)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0014444
Physical Location & Seq
Reverse (-)
2060183 .. 2062663
2481 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1024700.v1.1.491

Sequence Viewer

Length: 432 bp
ATGAGATTTATTTGGAATGTTGGGTTTCTTGAGCATATCATTGGGTGCACAGGGATTACCCAAACCAAAAGCCCTGAACAAAACCCTAGTCGAGTCCGAGTAGAAAAGAAAAAAAAAAGCCGCAGAGGCGCTGAAGAGAGAGAAGTGAGAAATAGGAAGAGAGAGATGACGCAGAAAGGGAAGCTGTTCAAAGGGCAGCAGAAGAGGAAAACGATTCCTCCCAACCGCCATGGAAAAATGACTCAAATTCGCAAAGGCAAGCGAGTAATCAAGCCTTCGAAGGTCACCGGCGAGATGGATTCCGATCGGGAGATGACAAAGTTCATAAACCACTGCAATGAGATGAAGGCGGCCACTGCTGCAAACAAGGAAGGCGGTCAATTAAGTATTGTCAAAACACCTCAAGCATCCGGTAACACTACAAAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2462

Protein Analysis

144

Amino Acids

16.36

Weight (kDa)

10.81

Isoelectric Point (pI)

35.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2462 PF09495 56 - 134 7.9e-07 Protein of unknown function (DUF2462)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014444)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52040
fragaria_vesca FvH4_7g01820
malus_domestica MD02G1300200.v1.1 MD07G1024700.v1.1
prunus_persica Prupe.2G024400_v2.0.a1
pyrus_communis pycom02g25220
rosa_chinensis RchiOBHm_Chr1g0318451
rosa_multiflora Rmu_sc0004803.1_g000001
rosa_roxburghii Rroxscaffold_4G00329240
rosa_rugosa Rorug01G0021400
rosa_samantha Rh1AG033500 Rh1BG029500 Rh1CG031800 Rh1CG033200 Rh1DG037300 Rh1DG037700
rosa_wichuraiana Rw1G002680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 121, 226, 350, 375
AcoI YGGCCR 1 cut(s) 351
AcsI RAATTY 1 cut(s) 246
AcuI CTGAAG 1 cut(s) 153
AgsI TTSAA 1 cut(s) 190
AluBI AGCT 1 cut(s) 184
AluI AGCT 1 cut(s) 184
Alw21I GWGCWC 1 cut(s) 50
Alw44I GTGCAC 1 cut(s) 46
AoxI GGCC 1 cut(s) 351
ApaLI GTGCAC 1 cut(s) 46
ApeKI GCWGC 2 cut(s) 196, 359
ApoI RAATTY 1 cut(s) 246
ArsI GACNNNNNNTTYG 2 cut(s) 73, 105
Asp700I GAANNNNTTC 1 cut(s) 185
AspLEI GCGC 1 cut(s) 131
AsuHPI GGTGA 1 cut(s) 277
AsuII TTCGAA 1 cut(s) 278
BaeGI GKGCMC 1 cut(s) 50
Bbv12I GWGCWC 1 cut(s) 50
BbvI GCAGC 2 cut(s) 208, 346
BccI CCATC 1 cut(s) 289
BfaI CTAG 1 cut(s) 87
BfoI RGCGCY 1 cut(s) 132
BglI GCCNNNNNGGC 1 cut(s) 126
BisI GCNGC 4 cut(s) 121, 197, 351, 360
BlsI GCNGC 4 cut(s) 122, 198, 352, 361
BmsI GCATC 1 cut(s) 416
Bpu14I TTCGAA 1 cut(s) 278
BpuEI CTTGAG 2 cut(s) 50, 387
BsaBI GATNNNNATC 1 cut(s) 303
BsaJI CCNNGG 1 cut(s) 229
BsaWI WCCGGW 1 cut(s) 410
BsaXI ACNNNNNCTCC 2 cut(s) 202, 232
Bse118I RCCGGY 1 cut(s) 287
Bse3DI GCAATG 1 cut(s) 343
Bse8I GATNNNNATC 1 cut(s) 303
BseDI CCNNGG 1 cut(s) 229
BseGI GGATG 1 cut(s) 407
BseJI GATNNNNATC 1 cut(s) 303
BseMI GCAATG 1 cut(s) 343
BseSI GKGCMC 1 cut(s) 50
BseXI GCAGC 2 cut(s) 208, 346
Bsh1285I CGRYCG 1 cut(s) 307
BshFI GGCC 1 cut(s) 353
BsiEI CGRYCG 1 cut(s) 307
BsiHKAI GWGCWC 1 cut(s) 50
BsiSI CCGG 2 cut(s) 288, 411
BsnI GGCC 1 cut(s) 353
Bsp119I TTCGAA 1 cut(s) 278
Bsp1286I GDGCHC 1 cut(s) 50
Bsp143I GATC 1 cut(s) 304
Bsp19I CCATGG 1 cut(s) 229
BspACI CCGC 4 cut(s) 121, 226, 350, 375
BspANI GGCC 1 cut(s) 353
BspT104I TTCGAA 1 cut(s) 278
BsrDI GCAATG 1 cut(s) 343
BsrFI RCCGGY 1 cut(s) 287
BssAI RCCGGY 1 cut(s) 287
BssECI CCNNGG 1 cut(s) 229
BssMI GATC 1 cut(s) 304
BssT1I CCWWGG 1 cut(s) 229
Bst6I CTCTTC 3 cut(s) 129, 152, 197
BstBI TTCGAA 1 cut(s) 278
BstC8I GCNNGC 1 cut(s) 260
BstDSI CCRYGG 1 cut(s) 229
BstEII GGTNACC 1 cut(s) 283
BstF5I GGATG 1 cut(s) 407
BstH2I RGCGCY 1 cut(s) 132
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 1 cut(s) 307
BstMBI GATC 1 cut(s) 304
BstMCI CGRYCG 1 cut(s) 307
BstMWI GCNNNNNNNGC 3 cut(s) 126, 356, 359
BstPI GGTNACC 1 cut(s) 283
BstSLI GKGCMC 1 cut(s) 50
BstV1I GCAGC 2 cut(s) 208, 346
BsuRI GGCC 1 cut(s) 353
BtgI CCRYGG 1 cut(s) 229
BtsCI GGATG 1 cut(s) 407
BtsI GCAGTG 2 cut(s) 331, 354
BtsIMutI CAGTG 2 cut(s) 331, 354
Cac8I GCNNGC 1 cut(s) 260
CfoI GCGC 1 cut(s) 131
Cfr10I RCCGGY 1 cut(s) 287
CseI GACGC 1 cut(s) 178
CviAII CATG 1 cut(s) 230
CviJI RGCY 5 cut(s) 72, 120, 184, 274, 353
CviKI_1 RGCY 5 cut(s) 72, 120, 184, 274, 353
DpnI GATC 1 cut(s) 306
DpnII GATC 1 cut(s) 304
EaeI YGGCCR 1 cut(s) 351
Eam1104I CTCTTC 3 cut(s) 129, 152, 197
EarI CTCTTC 3 cut(s) 129, 152, 197
Eco130I CCWWGG 1 cut(s) 229
Eco57I CTGAAG 1 cut(s) 153
Eco91I GGTNACC 1 cut(s) 283
EcoO65I GGTNACC 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 229
ErhI CCWWGG 1 cut(s) 229
FaeI CATG 1 cut(s) 233
FaiI YATR 3 cut(s) 36, 231, 326
FatI CATG 1 cut(s) 229
Fnu4HI GCNGC 4 cut(s) 121, 197, 351, 360
FokI GGATG 1 cut(s) 394
Fsp4HI GCNGC 4 cut(s) 121, 197, 351, 360
FspBI CTAG 1 cut(s) 87
GlaI GCGC 1 cut(s) 130
GluI GCNGC 4 cut(s) 121, 197, 351, 360
HaeII RGCGCY 1 cut(s) 132
HaeIII GGCC 1 cut(s) 353
HapII CCGG 2 cut(s) 288, 411
HgaI GACGC 1 cut(s) 178
HhaI GCGC 1 cut(s) 131
Hin1II CATG 1 cut(s) 233
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HinfI GANTC 4 cut(s) 93, 214, 241, 299
HpaII CCGG 2 cut(s) 288, 411
HphI GGTGA 1 cut(s) 277
Hpy166II GTNNAC 1 cut(s) 48
Hpy188I TCNGA 2 cut(s) 98, 304
Hpy188III TCNNGA 2 cut(s) 29, 308
Hpy8I GTNNAC 1 cut(s) 48
HpyAV CCTTC 4 cut(s) 274, 285, 340, 365
HpyCH4V TGCA 3 cut(s) 48, 336, 362
HpyF10VI GCNNNNNNNGC 3 cut(s) 126, 356, 359
Hsp92II CATG 1 cut(s) 233
HspAI GCGC 1 cut(s) 129
Kzo9I GATC 1 cut(s) 304
LpnPI CCDG 4 cut(s) 36, 87, 301, 424
Lsp1109I GCAGC 2 cut(s) 208, 346
LweI GCATC 1 cut(s) 416
MaeI CTAG 1 cut(s) 87
MaeIII GTNAC 2 cut(s) 283, 413
MalI GATC 1 cut(s) 306
MboI GATC 1 cut(s) 304
MboII GAAGA 3 cut(s) 146, 169, 214
MhlI GDGCHC 1 cut(s) 50
MluCI AATT 2 cut(s) 246, 380
MlyI GAGTC 2 cut(s) 102, 235
MnlI CCTC 4 cut(s) 119, 198, 228, 411
MroXI GAANNNNTTC 1 cut(s) 185
MseI TTAA 1 cut(s) 383
MslI CAYNNNNRTG 1 cut(s) 336
MspI CCGG 2 cut(s) 288, 411
MwoI GCNNNNNNNGC 3 cut(s) 126, 356, 359
NcoI CCATGG 1 cut(s) 229
NdeII GATC 1 cut(s) 304
NlaIII CATG 1 cut(s) 233
NmuCI GTSAC 1 cut(s) 283
NspV TTCGAA 1 cut(s) 278
PdmI GAANNNNTTC 1 cut(s) 185
PfeI GAWTC 2 cut(s) 214, 299
PkrI GCNGC 4 cut(s) 122, 198, 352, 361
Ple19I CGATCG 1 cut(s) 307
PleI GAGTC 2 cut(s) 101, 235
PpsI GAGTC 2 cut(s) 101, 235
PspEI GGTNACC 1 cut(s) 283
PvuI CGATCG 1 cut(s) 307
RseI CAYNNNNRTG 1 cut(s) 336
SaqAI TTAA 1 cut(s) 383
SatI GCNGC 4 cut(s) 121, 197, 351, 360
Sau3AI GATC 1 cut(s) 304
SchI GAGTC 2 cut(s) 102, 235
SduI GDGCHC 1 cut(s) 50
SetI ASST 3 cut(s) 186, 285, 403
SfaNI GCATC 1 cut(s) 416
SfuI TTCGAA 1 cut(s) 278
SgrAI CRCCGGYG 1 cut(s) 287
SmiMI CAYNNNNRTG 1 cut(s) 336
SmlI CTYRAG 2 cut(s) 29, 402
SmoI CTYRAG 2 cut(s) 29, 402
Sse9I AATT 2 cut(s) 246, 380
SsiI CCGC 4 cut(s) 121, 226, 350, 375
SspMI CTAG 1 cut(s) 87
StyI CCWWGG 1 cut(s) 229
TaqI TCGA 2 cut(s) 91, 278
TasI AATT 2 cut(s) 246, 380
TauI GCSGC 2 cut(s) 123, 353
TfiI GAWTC 2 cut(s) 214, 299
Tru1I TTAA 1 cut(s) 383
Tru9I TTAA 1 cut(s) 383
TscAI CASTG 2 cut(s) 338, 361
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 2 cut(s) 196, 359
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 2 cut(s) 313, 359
TspRI CASTG 2 cut(s) 338, 361
VneI GTGCAC 1 cut(s) 46
XapI RAATTY 1 cut(s) 246
XmnI GAANNNNTTC 1 cut(s) 185
XspI CTAG 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.