MD02G1318800.v1.1

Prokaryotic RING finger family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007795
Physical Location & Seq
Reverse (-)
37324856 .. 37326879
2024 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1318800.v1.1.491

Sequence Viewer

Length: 1296 bp
ATGGGGAACAATATAAGGGAAGAAATCATGATTTTGGATCTAAACCAAGAGCCTTTAGACCAACCACCTGATTCGGTGATCGGATTAGATAACATACTCAATGAGCTTGAAACTGCACATGGACGCATTGAAGAACGTATCAGACAACTAGAAGCAGTCACTTCCAGGGCTTCACAGCGTCCGAGGTGGCAACAAGCTCAAATTTCCCCTCAAATTGTGAATACCACTACCGGAGTTGTTGCTTCTGATACACAAAACGAACACAGTCAGAGTGGAGACGATGCTCTAGCTTCCCGAGAAACAGTGGAATCGGGCAAAACCAACAGAGGTGATAACATGCATTTGATAGCCAAGGCACTGGGGATGGATACACCTGTGAAAAAGGCAGAGCCTCATACTGGGAGCTTTTTTGATTGTAATATATGCTTGGACATGGCAAGAGATCCTATATTGACATGTTGCGGTCATCTGTTTTGTTGGCCATGCTTCTATCAGTTGTCATATGCGGATTCAAATGCAAAGGAATGCCCCGTGTGTGAAGGAGAAGTCACTGATACAAGTCTGATTCCAATTTACGGGAATGGGGATGGTAACTGTTCTCGCAAATCAAAGGAGTCTGTTTCAAAGGCCCCTCCTAGGCCCCCTGCACACAGGATTGAGGGTTTGAGGCAGCAGCTCATTAGGCGGGGCCCATCTTCTACTTTGATTGAAGAAAGGATTCAGCAACTTAGCAACATAGTTGGTTCAATGAGGGAGAGAAGACGGTCACGGGATAGTTCCCGTGCCCAAATCATGGCTGAAAGAACTAGTTTCGTGCCCAGCCAGAATCATGCATCCCAGGCATCACCTGCCACTGAGGCGAGAAACCAAAACCAGCATGATTCCCATCAAGTACTACAACGAGCTGCTTCAGTATCTTCCCTTTCGTCAGTTTTGAGTACTGCAATGGATTCAGCAGACAGATTAGTCGAGGATCTTGAGGAATATAGTATTAGTCCCCATGTGAGAAGAACCCATCATCAATCTTTACATATTGACACTGGAGATACATCTTCCAGCATTGTTGCAGTTATACAACCAGAAACTCTGACTTCTGATACTGCTGCTGAAATCAACTCTGATGCGCTCCCTTCTGCTTCCTTGATAAGAACTGATACCATTATTGTGGACCAGACAACAGATTCAGAAACAAATTTTGCATTGCTCCCCTCATCTTCTAGGAGAAGAACTAATCAAATAGATTTTGATTCAAACAATGGATATGCATCTGAACCTAGAAGAAGAAGGTTGAGATAA

Protein Analysis

432

Amino Acids

47.71

Weight (kDa)

5.59

Isoelectric Point (pI)

65.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_5 PF14634 138 - 180 3.6e-08 zinc-RING finger domain
zf-C3HC4_3 PF13920 139 - 185 1.3e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 139 - 179 6.1e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 139 - 179 1.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 139 - 177 4.4e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 856
AasI GACNNNNNNGTC 1 cut(s) 965
Acc36I ACCTGC 1 cut(s) 856
AccB7I CCANNNNNTGG 1 cut(s) 793
AciI CCGC 3 cut(s) 462, 506, 685
AclWI GGATC 3 cut(s) 45, 437, 981
AcoI YGGCCR 1 cut(s) 479
AcsI RAATTY 2 cut(s) 201, 1192
AcuI CTGAAG 1 cut(s) 894
AfaI GTAC 2 cut(s) 894, 940
AfiI CCNNNNNNNGG 4 cut(s) 398, 575, 636, 793
AflIII ACRYGT 1 cut(s) 455
AgsI TTSAA 7 cut(s) 110, 131, 513, 624, 710, 747, 1251
AhlI ACTAGT 1 cut(s) 806
AjnI CCWGG 2 cut(s) 164, 837
AluBI AGCT 6 cut(s) 106, 197, 290, 405, 676, 905
AluI AGCT 6 cut(s) 106, 197, 290, 405, 676, 905
Alw26I GTCTC 1 cut(s) 270
AlwI GGATC 3 cut(s) 45, 437, 981
Ama87I CYCGRG 1 cut(s) 294
AoxI GGCC 4 cut(s) 479, 627, 638, 688
ApaI GGGCCC 1 cut(s) 692
ApeKI GCWGC 4 cut(s) 670, 673, 905, 1103
ApoI RAATTY 2 cut(s) 201, 1192
Asp700I GAANNNNTTC 1 cut(s) 717
AspA2I CCTAGG 1 cut(s) 635
AspLEI GCGC 1 cut(s) 1126
AspS9I GGNCC 5 cut(s) 628, 639, 688, 689, 1168
AsuHPI GGTGA 3 cut(s) 88, 341, 837
AvaI CYCGRG 1 cut(s) 294
AvaII GGWCC 1 cut(s) 1168
AvrII CCTAGG 1 cut(s) 635
BaeGI GKGCMC 3 cut(s) 692, 787, 819
BaeI ACNNNNGTAYC 2 cut(s) 360, 393
BalI TGGCCA 1 cut(s) 481
BanII GRGCYC 1 cut(s) 692
BbsI GAAGAC 1 cut(s) 766
BbvI GCAGC 4 cut(s) 682, 685, 892, 1090
BccI CCATC 5 cut(s) 358, 581, 700, 894, 1023
BciT130I CCWGG 2 cut(s) 166, 839
BciVI GTATCC 1 cut(s) 361
BcoDI GTCTC 1 cut(s) 270
BcuI ACTAGT 1 cut(s) 806
BfaI CTAG 6 cut(s) 149, 287, 636, 807, 1218, 1275
BfuAI ACCTGC 1 cut(s) 856
BfuI GTATCC 1 cut(s) 361
BglI GCCNNNNNGGC 1 cut(s) 857
BisI GCNGC 4 cut(s) 671, 674, 906, 1104
BlnI CCTAGG 1 cut(s) 635
BlsI GCNGC 4 cut(s) 672, 675, 907, 1105
BmcAI AGTACT 2 cut(s) 894, 940
Bme1390I CCNGG 2 cut(s) 166, 839
Bme18I GGWCC 1 cut(s) 1168
BmeT110I CYCGRG 1 cut(s) 294
BmgT120I GGNCC 5 cut(s) 628, 639, 688, 689, 1168
BmiI GGNNCC 4 cut(s) 630, 641, 689, 690
BmrFI CCNGG 2 cut(s) 166, 839
BmrI ACTGGG 2 cut(s) 368, 408
BmsI GCATC 5 cut(s) 271, 842, 851, 1111, 1274
BmuI ACTGGG 2 cut(s) 368, 408
BpiI GAAGAC 1 cut(s) 766
BpmI CTGGAG 1 cut(s) 1062
BpuEI CTTGAG 1 cut(s) 998
BsaBI GATNNNNATC 2 cut(s) 885, 1264
BsaJI CCNNGG 5 cut(s) 165, 182, 351, 635, 837
BsaWI WCCGGW 1 cut(s) 230
Bsc4I CCNNNNNNNGG 4 cut(s) 398, 575, 636, 793
Bse1I ACTGG 3 cut(s) 363, 403, 1045
Bse3DI GCAATG 2 cut(s) 951, 1199
Bse8I GATNNNNATC 2 cut(s) 885, 1264
BseBI CCWGG 2 cut(s) 166, 839
BseDI CCNNGG 5 cut(s) 165, 182, 351, 635, 837
BseGI GGATG 3 cut(s) 369, 592, 833
BseJI GATNNNNATC 2 cut(s) 885, 1264
BseLI CCNNNNNNNGG 4 cut(s) 398, 575, 636, 793
BseMI GCAATG 2 cut(s) 951, 1199
BseMII CTCAG 1 cut(s) 846
BseNI ACTGG 3 cut(s) 363, 403, 1045
BseSI GKGCMC 3 cut(s) 692, 787, 819
BseXI GCAGC 4 cut(s) 682, 685, 892, 1090
BseYI CCCAGC 1 cut(s) 818
BsgI GTGCAG 2 cut(s) 99, 630
BshFI GGCC 4 cut(s) 481, 629, 640, 690
BsiHKCI CYCGRG 1 cut(s) 294
BsiSI CCGG 1 cut(s) 231
BslFI GGGAC 1 cut(s) 981
BslI CCNNNNNNNGG 4 cut(s) 398, 575, 636, 793
BsmAI GTCTC 1 cut(s) 270
BsmBI CGTCTC 1 cut(s) 270
BsmFI GGGAC 1 cut(s) 981
BsmI GAATGC 1 cut(s) 530
BsnI GGCC 4 cut(s) 481, 629, 640, 690
BsoBI CYCGRG 1 cut(s) 294
Bsp120I GGGCCC 1 cut(s) 688
Bsp1286I GDGCHC 3 cut(s) 692, 787, 819
Bsp143I GATC 4 cut(s) 37, 78, 442, 973
BspACI CCGC 3 cut(s) 462, 506, 685
BspANI GGCC 4 cut(s) 481, 629, 640, 690
BspCNI CTCAG 1 cut(s) 847
BspHI TCATGA 1 cut(s) 27
BspLI GGNNCC 4 cut(s) 630, 641, 689, 690
BspMI ACCTGC 1 cut(s) 856
BspPI GGATC 3 cut(s) 45, 437, 981
BsrDI GCAATG 2 cut(s) 951, 1199
BsrI ACTGG 3 cut(s) 363, 403, 1045
BssECI CCNNGG 5 cut(s) 165, 182, 351, 635, 837
BssMI GATC 4 cut(s) 37, 78, 442, 973
BssT1I CCWWGG 2 cut(s) 351, 635
Bst2UI CCWGG 2 cut(s) 166, 839
Bst4CI ACNGT 4 cut(s) 266, 304, 596, 765
BstAPI GCANNNNNTGC 1 cut(s) 848
BstDEI CTNAG 2 cut(s) 728, 855
BstF5I GGATG 3 cut(s) 369, 592, 833
BstHHI GCGC 1 cut(s) 1126
BstKTI GATC 4 cut(s) 40, 81, 445, 976
BstMAI GTCTC 1 cut(s) 270
BstMBI GATC 4 cut(s) 37, 78, 442, 973
BstMWI GCNNNNNNNGC 4 cut(s) 682, 839, 848, 857
BstNI CCWGG 2 cut(s) 166, 839
BstNSI RCATGY 2 cut(s) 340, 459
BstSCI CCNGG 2 cut(s) 164, 837
BstSLI GKGCMC 3 cut(s) 692, 787, 819
BstV1I GCAGC 4 cut(s) 682, 685, 892, 1090
BstV2I GAAGAC 1 cut(s) 766
BstX2I RGATCY 3 cut(s) 37, 442, 973
BstXI CCANNNNNNTGG 2 cut(s) 358, 1165
BstYI RGATCY 3 cut(s) 37, 442, 973
BsuI GTATCC 1 cut(s) 361
BsuRI GGCC 4 cut(s) 481, 629, 640, 690
BtsCI GGATG 3 cut(s) 369, 592, 833
BtsIMutI CAGTG 5 cut(s) 309, 356, 549, 852, 1038
BveI ACCTGC 1 cut(s) 856
CciI TCATGA 1 cut(s) 27
CfoI GCGC 1 cut(s) 1126
Cfr13I GGNCC 5 cut(s) 628, 639, 688, 689, 1168
CseI GACGC 2 cut(s) 132, 167
Csp6I GTAC 2 cut(s) 893, 939
CviQI GTAC 2 cut(s) 893, 939
DdeI CTNAG 2 cut(s) 728, 855
DpnI GATC 4 cut(s) 39, 80, 444, 975
DpnII GATC 4 cut(s) 37, 78, 442, 973
DrdI GACNNNNNNGTC 1 cut(s) 965
DseDI GACNNNNNNGTC 1 cut(s) 965
EaeI YGGCCR 1 cut(s) 479
Eco130I CCWWGG 2 cut(s) 351, 635
Eco24I GRGCYC 1 cut(s) 692
Eco47I GGWCC 1 cut(s) 1168
Eco57I CTGAAG 1 cut(s) 894
Eco88I CYCGRG 1 cut(s) 294
EcoO109I RGGNCCY 3 cut(s) 628, 639, 688
EcoRII CCWGG 2 cut(s) 164, 837
EcoT14I CCWWGG 2 cut(s) 351, 635
EcoT22I ATGCAT 3 cut(s) 342, 835, 1267
EcoT38I GRGCYC 1 cut(s) 692
ErhI CCWWGG 2 cut(s) 351, 635
Esp3I CGTCTC 1 cut(s) 270
FaqI GGGAC 1 cut(s) 981
FauI CCCGC 1 cut(s) 678
FauNDI CATATG 1 cut(s) 502
Fnu4HI GCNGC 4 cut(s) 671, 674, 906, 1104
FokI GGATG 3 cut(s) 376, 599, 820
FriOI GRGCYC 1 cut(s) 692
Fsp4HI GCNGC 4 cut(s) 671, 674, 906, 1104
FspBI CTAG 6 cut(s) 149, 287, 636, 807, 1218, 1275
GlaI GCGC 1 cut(s) 1125
GluI GCNGC 4 cut(s) 671, 674, 906, 1104
GsaI CCCAGC 1 cut(s) 822
GsuI CTGGAG 1 cut(s) 1062
HaeIII GGCC 4 cut(s) 481, 629, 640, 690
HapII CCGG 1 cut(s) 231
HgaI GACGC 2 cut(s) 132, 167
HhaI GCGC 1 cut(s) 1126
Hin6I GCGC 1 cut(s) 1124
HinP1I GCGC 1 cut(s) 1124
HpaII CCGG 1 cut(s) 231
HphI GGTGA 3 cut(s) 88, 341, 837
Hpy166II GTNNAC 1 cut(s) 1168
Hpy188III TCNNGA 3 cut(s) 28, 294, 977
Hpy8I GTNNAC 1 cut(s) 1168
HpyAV CCTTC 3 cut(s) 533, 1140, 1278
HpyCH4III ACNGT 4 cut(s) 266, 304, 596, 765
HpyCH4IV ACGT 1 cut(s) 136
HpyCH4V TGCA 9 cut(s) 116, 340, 518, 647, 833, 944, 1067, 1199, 1265
HpyF10VI GCNNNNNNNGC 4 cut(s) 682, 839, 848, 857
HpyF3I CTNAG 2 cut(s) 728, 855
HpySE526I ACGT 1 cut(s) 136
HspAI GCGC 1 cut(s) 1124
Kzo9I GATC 4 cut(s) 37, 78, 442, 973
LmnI GCTCC 3 cut(s) 402, 1131, 1209
Lsp1109I GCAGC 4 cut(s) 682, 685, 892, 1090
LweI GCATC 5 cut(s) 271, 842, 851, 1111, 1274
MaeI CTAG 6 cut(s) 149, 287, 636, 807, 1218, 1275
MaeII ACGT 1 cut(s) 136
MaeIII GTNAC 4 cut(s) 157, 547, 590, 765
MalI GATC 4 cut(s) 39, 80, 444, 975
MboI GATC 4 cut(s) 37, 78, 442, 973
MflI RGATCY 3 cut(s) 37, 442, 973
MhlI GDGCHC 3 cut(s) 692, 787, 819
MlsI TGGCCA 1 cut(s) 481
MluCI AATT 4 cut(s) 201, 213, 570, 1192
MluNI TGGCCA 1 cut(s) 481
MlyI GAGTC 1 cut(s) 623
Mox20I TGGCCA 1 cut(s) 481
Mph1103I ATGCAT 3 cut(s) 342, 835, 1267
MroXI GAANNNNTTC 1 cut(s) 717
MscI TGGCCA 1 cut(s) 481
MslI CAYNNNNRTG 1 cut(s) 1163
Msp20I TGGCCA 1 cut(s) 481
MspI CCGG 1 cut(s) 231
MspR9I CCNGG 2 cut(s) 166, 839
Mva1269I GAATGC 1 cut(s) 530
MvaI CCWGG 2 cut(s) 166, 839
MwoI GCNNNNNNNGC 4 cut(s) 682, 839, 848, 857
NdeI CATATG 1 cut(s) 502
NdeII GATC 4 cut(s) 37, 78, 442, 973
NlaIV GGNNCC 4 cut(s) 630, 641, 689, 690
NmuCI GTSAC 3 cut(s) 157, 547, 765
NsiI ATGCAT 3 cut(s) 342, 835, 1267
NspI RCATGY 2 cut(s) 340, 459
PagI TCATGA 1 cut(s) 27
PaqCI CACCTGC 1 cut(s) 856
PciI ACATGT 1 cut(s) 455
PctI GAATGC 1 cut(s) 530
PdmI GAANNNNTTC 1 cut(s) 717
PflMI CCANNNNNTGG 1 cut(s) 793
PkrI GCNGC 4 cut(s) 672, 675, 907, 1105
PleI GAGTC 1 cut(s) 622
PpsI GAGTC 1 cut(s) 622
PscI ACATGT 1 cut(s) 455
Psp6I CCWGG 2 cut(s) 164, 837
PspFI CCCAGC 1 cut(s) 818
PspGI CCWGG 2 cut(s) 164, 837
PspN4I GGNNCC 4 cut(s) 630, 641, 689, 690
PspOMI GGGCCC 1 cut(s) 688
PspPI GGNCC 5 cut(s) 628, 639, 688, 689, 1168
PsuI RGATCY 3 cut(s) 37, 442, 973
RsaI GTAC 2 cut(s) 894, 940
RsaNI GTAC 2 cut(s) 893, 939
RseI CAYNNNNRTG 1 cut(s) 1163
SatI GCNGC 4 cut(s) 671, 674, 906, 1104
Sau3AI GATC 4 cut(s) 37, 78, 442, 973
Sau96I GGNCC 5 cut(s) 628, 639, 688, 689, 1168
ScaI AGTACT 2 cut(s) 894, 940
SchI GAGTC 1 cut(s) 623
ScrFI CCNGG 2 cut(s) 166, 839
SduI GDGCHC 3 cut(s) 692, 787, 819
SfaNI GCATC 5 cut(s) 271, 842, 851, 1111, 1274
SinI GGWCC 1 cut(s) 1168
SmiMI CAYNNNNRTG 1 cut(s) 1163
SmlI CTYRAG 1 cut(s) 977
SmoI CTYRAG 1 cut(s) 977
SpeI ACTAGT 1 cut(s) 806
Sse9I AATT 4 cut(s) 201, 213, 570, 1192
SsiI CCGC 3 cut(s) 462, 506, 685
SspMI CTAG 6 cut(s) 149, 287, 636, 807, 1218, 1275
StyD4I CCNGG 2 cut(s) 164, 837
StyI CCWWGG 2 cut(s) 351, 635
TaaI ACNGT 4 cut(s) 266, 304, 596, 765
TaiI ACGT 1 cut(s) 139
TaqI TCGA 1 cut(s) 969
TasI AATT 4 cut(s) 201, 213, 570, 1192
TatI WGTACW 2 cut(s) 892, 938
TscAI CASTG 5 cut(s) 309, 363, 556, 859, 1045
TseFI GTSAC 3 cut(s) 157, 547, 765
TseI GCWGC 4 cut(s) 670, 673, 905, 1103
Tsp45I GTSAC 3 cut(s) 157, 547, 765
TspRI CASTG 5 cut(s) 309, 363, 556, 859, 1045
Van91I CCANNNNNTGG 1 cut(s) 793
VpaK11BI GGWCC 1 cut(s) 1168
XapI RAATTY 2 cut(s) 201, 1192
XceI RCATGY 2 cut(s) 340, 459
XmaJI CCTAGG 1 cut(s) 635
XmnI GAANNNNTTC 1 cut(s) 717
XspI CTAG 6 cut(s) 149, 287, 636, 807, 1218, 1275
ZrmI AGTACT 2 cut(s) 894, 940
Zsp2I ATGCAT 3 cut(s) 342, 835, 1267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.