pycom02g26710

Prokaryotic RING finger family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0007795
Physical Location & Seq
Reverse (-)
24512335 .. 24513642
1308 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g26710.1

Sequence Viewer

Length: 1308 bp
ATGGGGAGCAATATAAGGGAAGAAATCATGATTTTGGATCTAAACCAAGAGCCTTTAGACCAACCACCTGATTCGGTGATCGGATTAGATAACATACTCAATGAGCTTGAAACTGCACATGGACGCATTGAAGAACGTATCAGACAACTAGAAGCAGTCACTTCCAGGGCTTCGCAGCGTCCGAGGTGGCGACAAGCTCAAATTTCCCCTCAAATTGTGAATATCACTGCCGGAGTTGTTGCTTCTGATACACAAAACGAACACAGTCAGAGTGGAGACGATGCTCTAGCTTCCCGAGAAACAGTGGAATCGGGCAAAACCAACAGAGGTGATAACATGCATTTGATAGCCAAGGCACTGGGGATGGATACACCTCTGAAAAAGGCAGAGCCTCATACTGGGAGCTTTTTTGATTGTAATATATGCTTGGACATGGCAAGAGATCCTATATTGACATGTTGCGGTCATCTGTTTTGTTGGCCATGCTTCTATCAGTTGTCATATGCGGATTCAAATGCAAAGGAATGCCCCGTGTGTGAAGGAGAAGTCACTGATACAAGTCTGATTCCAATTTACGGGAATGGGGATGGTAACTGTTCTCGCAAATCAAAGGAGTCTGTTTCAAAGGCCCCTCCTAGGCCCCCTGCACACAGGATTGAGGGTTTGAGGCAACAGCTCATTAGGTGGGGCCCATCTTCTACTTTGATTGAAGAAAGGATTCAGCAACTTAGCAACATAGTTGGTTCAATGAGGGAGAGAAGACGGTCACGGGATAGTTCCCCTGCCCAAATCATGGCTGAAAGAACTAGTTTCGTGCCCAGCCAGAATCATGCATCCCAGGCATCACCTGCCACTGAGGCGAGAAACCAAAACCAGCATGATTCCCTTCAAGTTTCAAGATTATTACTACAACGAGCTGCTTCAGTATCTTCCCTTTCGTCAGCTTTGAGTACTGCAATGGATTCGGCAGAAAGATTAGTCGAGGATCTCGAGGAATATAGTATTAGTCACCATGTGAGAAGAACCCATCATCAATCTTTACATATTGACAATGGAGATACATCTTCCAGCATTGCTGCAGTTATACAACCAGAAACTCTTACTTCTGATACTGCTGCTGAAATCAACTCTGATGCGCTCCCTTCTGCTTCCTTTATAAGAACTGAGACCGTTATTGTGGACCAGACAACAGATTCAGAAACAAATTTTGCATTGCTCCCCTCATCTTCTAGGAGAAGAACTAATCAAATAGATTTTGATTCAAACAATGCATATGCATCTGAACCTAGAAGAAGAAGGTTGAGATAA

Protein Analysis

436

Amino Acids

48.18

Weight (kDa)

5.59

Isoelectric Point (pI)

69.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_5 PF14634 138 - 180 3.7e-08 zinc-RING finger domain
zf-C3HC4_3 PF13920 139 - 185 1.3e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 139 - 179 6.2e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 139 - 179 1.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 139 - 177 4.5e-06 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1157
AarI CACCTGC 1 cut(s) 856
Acc36I ACCTGC 1 cut(s) 856
AccB7I CCANNNNNTGG 1 cut(s) 793
AciI CCGC 2 cut(s) 462, 506
AclWI GGATC 3 cut(s) 45, 437, 993
AcoI YGGCCR 1 cut(s) 479
AcsI RAATTY 2 cut(s) 201, 1204
AcuI CTGAAG 1 cut(s) 906
AdeI CACNNNGTG 1 cut(s) 1015
AfaI GTAC 1 cut(s) 952
AfiI CCNNNNNNNGG 4 cut(s) 398, 575, 636, 793
AflIII ACRYGT 1 cut(s) 455
AgsI TTSAA 9 cut(s) 110, 131, 513, 624, 710, 747, 890, 897, 1263
AhlI ACTAGT 1 cut(s) 806
AjnI CCWGG 2 cut(s) 164, 837
AluBI AGCT 7 cut(s) 106, 197, 290, 405, 676, 917, 944
AluI AGCT 7 cut(s) 106, 197, 290, 405, 676, 917, 944
Alw26I GTCTC 2 cut(s) 270, 1160
AlwI GGATC 3 cut(s) 45, 437, 993
Ama87I CYCGRG 2 cut(s) 294, 989
AoxI GGCC 4 cut(s) 479, 627, 638, 688
ApaI GGGCCC 1 cut(s) 692
ApeKI GCWGC 4 cut(s) 175, 917, 1076, 1115
ApoI RAATTY 2 cut(s) 201, 1204
Asp700I GAANNNNTTC 1 cut(s) 717
AspA2I CCTAGG 1 cut(s) 635
AspLEI GCGC 1 cut(s) 1138
AspS9I GGNCC 5 cut(s) 628, 639, 688, 689, 1180
AsuHPI GGTGA 4 cut(s) 88, 341, 837, 1001
AvaI CYCGRG 2 cut(s) 294, 989
AvaII GGWCC 1 cut(s) 1180
AvrII CCTAGG 1 cut(s) 635
BaeGI GKGCMC 2 cut(s) 692, 819
BalI TGGCCA 1 cut(s) 481
BanII GRGCYC 1 cut(s) 692
BbsI GAAGAC 1 cut(s) 766
BbvI GCAGC 4 cut(s) 187, 904, 1063, 1102
BccI CCATC 4 cut(s) 358, 581, 700, 1035
BcgI CGANNNNNNTGC 2 cut(s) 945, 979
BciT130I CCWGG 2 cut(s) 166, 839
BciVI GTATCC 1 cut(s) 361
BcoDI GTCTC 2 cut(s) 270, 1160
BcuI ACTAGT 1 cut(s) 806
BfaI CTAG 6 cut(s) 149, 287, 636, 807, 1230, 1287
BfmI CTRYAG 1 cut(s) 1077
BfuAI ACCTGC 1 cut(s) 856
BfuI GTATCC 1 cut(s) 361
BglI GCCNNNNNGGC 1 cut(s) 857
BisI GCNGC 4 cut(s) 176, 918, 1077, 1116
BlnI CCTAGG 1 cut(s) 635
BlsI GCNGC 4 cut(s) 177, 919, 1078, 1117
BmcAI AGTACT 1 cut(s) 952
Bme1390I CCNGG 2 cut(s) 166, 839
Bme18I GGWCC 1 cut(s) 1180
BmeT110I CYCGRG 2 cut(s) 294, 989
BmgT120I GGNCC 5 cut(s) 628, 639, 688, 689, 1180
BmiI GGNNCC 4 cut(s) 630, 641, 689, 690
BmrFI CCNGG 2 cut(s) 166, 839
BmrI ACTGGG 2 cut(s) 368, 408
BmsI GCATC 5 cut(s) 271, 842, 851, 1123, 1286
BmuI ACTGGG 2 cut(s) 368, 408
BpiI GAAGAC 1 cut(s) 766
BsaI GGTCTC 1 cut(s) 1160
BsaJI CCNNGG 5 cut(s) 165, 182, 351, 635, 837
Bsc4I CCNNNNNNNGG 4 cut(s) 398, 575, 636, 793
Bse1I ACTGG 2 cut(s) 363, 403
Bse3DI GCAATG 3 cut(s) 963, 1071, 1211
BseBI CCWGG 2 cut(s) 166, 839
BseDI CCNNGG 5 cut(s) 165, 182, 351, 635, 837
BseGI GGATG 3 cut(s) 369, 592, 833
BseLI CCNNNNNNNGG 4 cut(s) 398, 575, 636, 793
BseMI GCAATG 3 cut(s) 963, 1071, 1211
BseMII CTCAG 2 cut(s) 846, 1155
BseNI ACTGG 2 cut(s) 363, 403
BseSI GKGCMC 2 cut(s) 692, 819
BseXI GCAGC 4 cut(s) 187, 904, 1063, 1102
BseYI CCCAGC 1 cut(s) 818
BsgI GTGCAG 2 cut(s) 99, 630
BshFI GGCC 4 cut(s) 481, 629, 640, 690
BsiHKCI CYCGRG 2 cut(s) 294, 989
BsiSI CCGG 1 cut(s) 231
BslI CCNNNNNNNGG 4 cut(s) 398, 575, 636, 793
BsmAI GTCTC 2 cut(s) 270, 1160
BsmBI CGTCTC 1 cut(s) 270
BsmI GAATGC 1 cut(s) 530
BsnI GGCC 4 cut(s) 481, 629, 640, 690
Bso31I GGTCTC 1 cut(s) 1160
BsoBI CYCGRG 2 cut(s) 294, 989
Bsp120I GGGCCC 1 cut(s) 688
Bsp1286I GDGCHC 2 cut(s) 692, 819
Bsp143I GATC 4 cut(s) 37, 78, 442, 985
BspACI CCGC 2 cut(s) 462, 506
BspANI GGCC 4 cut(s) 481, 629, 640, 690
BspCNI CTCAG 2 cut(s) 847, 1156
BspHI TCATGA 1 cut(s) 27
BspLI GGNNCC 4 cut(s) 630, 641, 689, 690
BspMAI CTGCAG 1 cut(s) 1081
BspMI ACCTGC 1 cut(s) 856
BspPI GGATC 3 cut(s) 45, 437, 993
BspTNI GGTCTC 1 cut(s) 1160
BsrDI GCAATG 3 cut(s) 963, 1071, 1211
BsrI ACTGG 2 cut(s) 363, 403
BssECI CCNNGG 5 cut(s) 165, 182, 351, 635, 837
BssMI GATC 4 cut(s) 37, 78, 442, 985
BssT1I CCWWGG 2 cut(s) 351, 635
Bst2UI CCWGG 2 cut(s) 166, 839
Bst4CI ACNGT 5 cut(s) 266, 304, 596, 765, 1171
BstAPI GCANNNNNTGC 1 cut(s) 848
BstDEI CTNAG 3 cut(s) 728, 855, 1164
BstF5I GGATG 3 cut(s) 369, 592, 833
BstHHI GCGC 1 cut(s) 1138
BstKTI GATC 4 cut(s) 40, 81, 445, 988
BstMAI GTCTC 2 cut(s) 270, 1160
BstMBI GATC 4 cut(s) 37, 78, 442, 985
BstMWI GCNNNNNNNGC 3 cut(s) 839, 848, 857
BstNI CCWGG 2 cut(s) 166, 839
BstNSI RCATGY 2 cut(s) 340, 459
BstSCI CCNGG 2 cut(s) 164, 837
BstSFI CTRYAG 1 cut(s) 1077
BstSLI GKGCMC 2 cut(s) 692, 819
BstV1I GCAGC 4 cut(s) 187, 904, 1063, 1102
BstV2I GAAGAC 1 cut(s) 766
BstX2I RGATCY 3 cut(s) 37, 442, 985
BstXI CCANNNNNNTGG 1 cut(s) 358
BstYI RGATCY 3 cut(s) 37, 442, 985
BsuI GTATCC 1 cut(s) 361
BsuRI GGCC 4 cut(s) 481, 629, 640, 690
BtsCI GGATG 3 cut(s) 369, 592, 833
BtsI GCAGTG 1 cut(s) 225
BtsIMutI CAGTG 5 cut(s) 225, 309, 356, 549, 852
BveI ACCTGC 1 cut(s) 856
CciI TCATGA 1 cut(s) 27
CfoI GCGC 1 cut(s) 1138
Cfr13I GGNCC 5 cut(s) 628, 639, 688, 689, 1180
CseI GACGC 2 cut(s) 132, 167
Csp6I GTAC 1 cut(s) 951
CviQI GTAC 1 cut(s) 951
DdeI CTNAG 3 cut(s) 728, 855, 1164
DpnI GATC 4 cut(s) 39, 80, 444, 987
DpnII GATC 4 cut(s) 37, 78, 442, 985
DraIII CACNNNGTG 1 cut(s) 1015
EaeI YGGCCR 1 cut(s) 479
Eco130I CCWWGG 2 cut(s) 351, 635
Eco24I GRGCYC 1 cut(s) 692
Eco31I GGTCTC 1 cut(s) 1160
Eco47I GGWCC 1 cut(s) 1180
Eco57I CTGAAG 1 cut(s) 906
Eco88I CYCGRG 2 cut(s) 294, 989
EcoO109I RGGNCCY 3 cut(s) 628, 639, 688
EcoRII CCWGG 2 cut(s) 164, 837
EcoT14I CCWWGG 2 cut(s) 351, 635
EcoT22I ATGCAT 4 cut(s) 342, 835, 1273, 1279
EcoT38I GRGCYC 1 cut(s) 692
ErhI CCWWGG 2 cut(s) 351, 635
Esp3I CGTCTC 1 cut(s) 270
FauNDI CATATG 2 cut(s) 502, 1273
Fnu4HI GCNGC 4 cut(s) 176, 918, 1077, 1116
FokI GGATG 3 cut(s) 376, 599, 820
FriOI GRGCYC 1 cut(s) 692
Fsp4HI GCNGC 4 cut(s) 176, 918, 1077, 1116
FspBI CTAG 6 cut(s) 149, 287, 636, 807, 1230, 1287
GlaI GCGC 1 cut(s) 1137
GluI GCNGC 4 cut(s) 176, 918, 1077, 1116
GsaI CCCAGC 1 cut(s) 822
HaeIII GGCC 4 cut(s) 481, 629, 640, 690
HapII CCGG 1 cut(s) 231
HgaI GACGC 2 cut(s) 132, 167
HhaI GCGC 1 cut(s) 1138
Hin6I GCGC 1 cut(s) 1136
HinP1I GCGC 1 cut(s) 1136
HpaII CCGG 1 cut(s) 231
HphI GGTGA 4 cut(s) 88, 341, 837, 1001
Hpy166II GTNNAC 1 cut(s) 1180
Hpy188III TCNNGA 4 cut(s) 28, 294, 897, 989
Hpy8I GTNNAC 1 cut(s) 1180
HpyAV CCTTC 4 cut(s) 533, 896, 1152, 1290
HpyCH4III ACNGT 5 cut(s) 266, 304, 596, 765, 1171
HpyCH4IV ACGT 1 cut(s) 136
HpyF10VI GCNNNNNNNGC 3 cut(s) 839, 848, 857
HpyF3I CTNAG 3 cut(s) 728, 855, 1164
HpySE526I ACGT 1 cut(s) 136
HspAI GCGC 1 cut(s) 1136
Kzo9I GATC 4 cut(s) 37, 78, 442, 985
LmnI GCTCC 4 cut(s) 6, 402, 1143, 1221
Lsp1109I GCAGC 4 cut(s) 187, 904, 1063, 1102
LweI GCATC 5 cut(s) 271, 842, 851, 1123, 1286
MaeI CTAG 6 cut(s) 149, 287, 636, 807, 1230, 1287
MaeII ACGT 1 cut(s) 136
MaeIII GTNAC 5 cut(s) 157, 547, 590, 765, 1007
MalI GATC 4 cut(s) 39, 80, 444, 987
MboI GATC 4 cut(s) 37, 78, 442, 985
MflI RGATCY 3 cut(s) 37, 442, 985
MhlI GDGCHC 2 cut(s) 692, 819
MlsI TGGCCA 1 cut(s) 481
MluCI AATT 4 cut(s) 201, 213, 570, 1204
MluNI TGGCCA 1 cut(s) 481
MlyI GAGTC 1 cut(s) 623
Mox20I TGGCCA 1 cut(s) 481
Mph1103I ATGCAT 4 cut(s) 342, 835, 1273, 1279
MroXI GAANNNNTTC 1 cut(s) 717
MscI TGGCCA 1 cut(s) 481
Msp20I TGGCCA 1 cut(s) 481
MspI CCGG 1 cut(s) 231
MspR9I CCNGG 2 cut(s) 166, 839
Mva1269I GAATGC 1 cut(s) 530
MvaI CCWGG 2 cut(s) 166, 839
MwoI GCNNNNNNNGC 3 cut(s) 839, 848, 857
NdeI CATATG 2 cut(s) 502, 1273
NdeII GATC 4 cut(s) 37, 78, 442, 985
NlaIV GGNNCC 4 cut(s) 630, 641, 689, 690
NmuCI GTSAC 4 cut(s) 157, 547, 765, 1007
NsiI ATGCAT 4 cut(s) 342, 835, 1273, 1279
NspI RCATGY 2 cut(s) 340, 459
PaeR7I CTCGAG 1 cut(s) 989
PagI TCATGA 1 cut(s) 27
PaqCI CACCTGC 1 cut(s) 856
PciI ACATGT 1 cut(s) 455
PcsI WCGNNNNNNNCGW 2 cut(s) 179, 987
PctI GAATGC 1 cut(s) 530
PdmI GAANNNNTTC 1 cut(s) 717
PflMI CCANNNNNTGG 1 cut(s) 793
PkrI GCNGC 4 cut(s) 177, 919, 1078, 1117
PleI GAGTC 1 cut(s) 622
PpsI GAGTC 1 cut(s) 622
PscI ACATGT 1 cut(s) 455
PsiI TTATAA 1 cut(s) 1157
Psp6I CCWGG 2 cut(s) 164, 837
PspFI CCCAGC 1 cut(s) 818
PspGI CCWGG 2 cut(s) 164, 837
PspN4I GGNNCC 4 cut(s) 630, 641, 689, 690
PspOMI GGGCCC 1 cut(s) 688
PspPI GGNCC 5 cut(s) 628, 639, 688, 689, 1180
PstI CTGCAG 1 cut(s) 1081
PsuI RGATCY 3 cut(s) 37, 442, 985
RsaI GTAC 1 cut(s) 952
RsaNI GTAC 1 cut(s) 951
SatI GCNGC 4 cut(s) 176, 918, 1077, 1116
Sau3AI GATC 4 cut(s) 37, 78, 442, 985
Sau96I GGNCC 5 cut(s) 628, 639, 688, 689, 1180
ScaI AGTACT 1 cut(s) 952
SchI GAGTC 1 cut(s) 623
ScrFI CCNGG 2 cut(s) 166, 839
SduI GDGCHC 2 cut(s) 692, 819
SfaNI GCATC 5 cut(s) 271, 842, 851, 1123, 1286
SfcI CTRYAG 1 cut(s) 1077
Sfr274I CTCGAG 1 cut(s) 989
SinI GGWCC 1 cut(s) 1180
SlaI CTCGAG 1 cut(s) 989
SmlI CTYRAG 1 cut(s) 989
SmoI CTYRAG 1 cut(s) 989
SpeI ACTAGT 1 cut(s) 806
Sse9I AATT 4 cut(s) 201, 213, 570, 1204
SsiI CCGC 2 cut(s) 462, 506
SspMI CTAG 6 cut(s) 149, 287, 636, 807, 1230, 1287
StyD4I CCNGG 2 cut(s) 164, 837
StyI CCWWGG 2 cut(s) 351, 635
TaaI ACNGT 5 cut(s) 266, 304, 596, 765, 1171
TaiI ACGT 1 cut(s) 139
TaqI TCGA 2 cut(s) 981, 990
TasI AATT 4 cut(s) 201, 213, 570, 1204
TatI WGTACW 1 cut(s) 950
TscAI CASTG 5 cut(s) 232, 309, 363, 556, 859
TseFI GTSAC 4 cut(s) 157, 547, 765, 1007
TseI GCWGC 4 cut(s) 175, 917, 1076, 1115
Tsp45I GTSAC 4 cut(s) 157, 547, 765, 1007
TspRI CASTG 5 cut(s) 232, 309, 363, 556, 859
Van91I CCANNNNNTGG 1 cut(s) 793
VpaK11BI GGWCC 1 cut(s) 1180
XapI RAATTY 2 cut(s) 201, 1204
XceI RCATGY 2 cut(s) 340, 459
XhoI CTCGAG 1 cut(s) 989
XmaJI CCTAGG 1 cut(s) 635
XmnI GAANNNNTTC 1 cut(s) 717
XspI CTAG 6 cut(s) 149, 287, 636, 807, 1230, 1287
ZrmI AGTACT 1 cut(s) 952
Zsp2I ATGCAT 4 cut(s) 342, 835, 1273, 1279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.