Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0007795

Physical Location & Seq

Genomic Coordinates Forward (+)

261730 .. 264341

Gene Structure 2612 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1002400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1311 bp

ATGGAGATGGGGAGTAATATAAGGGAAGAAATCATGATTTTGGATTTAAACCAAGAGCCTTTAGACCAACTGCCTGATTCGGTGATCGGATTAGATAACATACTCGATGAGCTTGAAACTGCCCATGGACGCATTGAAGAACGTATCAGACAACTAGAAGCAGTCACTTCCAGGGCTAGGCAGCGTCAGAGGTGGCGACAAGCTCAAATTTCCCCTCAAATTGTGAATATCACTGCTGGTGTTGTTGCTTCTGATACACAAAACGAACACAGTCAGAATGAGGACGATGCTCCAGCTGCCCGAGAAACAGTGGAATCTGGCAAAACCAACAGAGGTGATAACATGCATTTGATAGCCAAGGCACTGGGGATGGATACAAATGTGAAAAAGGCAGAGCCTCATACTGGGAGCTTTTTTGATTGTCATATATGCTTGGATATGGCAAGAGATCCTATATTGACATGTTGCGGTCATTTGTTTTGTTGGCCTTGCTTCTATCAGTTGTCGTATGCGGATTCAAATGCAAAGGAATGCCCCGTGTGTAAGGGAGAAGTAACTGATACAAGTCTGATTCCAATTTACGGGAATGGGGATGGTAAATGTTTTCGCAAGTCAAACGAGTCTGTTTCAAAGGCCCCTCCTAGGCCCCCTGCACACAGGATTGAGGGTTTGAGGCAGCAGCGTATTAGGCGGGGCCCATCTTCTACATTGATTGAAGAAAGGATTCAGCAACTTAGCAACATAGTTGGTTCAATGGGGGAGAGAAGACGGTCACGGGATAGTTTCCATGCACAAGTCATGGCTGAAAGAACTAGTTTCGTGCCCAGCCAGGACCAGACATCACAGGCATCACCTGCCACTGAGACAAGAAACCAACACCAGCATGATTCCCTTCAAGTTTCAAGATTATTACTACAAGGAGCCGCTTCAGTATCTTCCCTTTCATCAGCATTGAATACTGCATTGGATTCAGCAGAAAGATTAGTCGAGGACCTCGAGGAATATAGTAATGGTCACCATGTTAGAAGAAACCGTCATCAATCTTTACATATTGATAATGGAGATACATCTGTGAGCATTGCTGCAGTTATACAACCAGAAACGCAGACTTCTGATACTGCTGCTGAAATCAACTCTGATGCGCTCCCCTCTGCTTCCTTTATAAGAACTGATACCATTATTGTGGATCAGCCAACAGGTTCAGAAACAAATTTTGCATTGCATCCCTCATCTTCTAGGAGAACTAATTCAAGAGGTTCTGATTCAAACAATGGATATACATCTGAACCTAGAAGGAGAAGGTTGAGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006508 GO:0006515 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010033 GO:0010243 GO:0010498 GO:0012505 GO:0016020 GO:0019538 GO:0019899 GO:0030163 GO:0031984 GO:0032991 GO:0033554 GO:0034976 GO:0035966 GO:0035967 GO:0036503 GO:0036513 GO:0042175 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044390 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0050896 GO:0051603 GO:0051716 GO:0051788 GO:0070887 GO:0071218 GO:0071310 GO:0071704 GO:0071712 GO:0098796 GO:0098827 GO:1901564 GO:1901565 GO:1901575 GO:1901698

KEGG Pathways

Metabolic & Signaling

ko04141 map04141

Pfam Domains

Protein Families

zf-C3HC4_3 zf-RING_5

Protein Analysis

437

Amino Acids

48.43

Weight (kDa)

5.86

Isoelectric Point (pI)

62.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-C3HC4_3	PF13920	141 - 187	1.3e-07	Zinc finger, C3HC4 type (RING finger)
zf-RING_5	PF14634	141 - 182	5.7e-07	zinc-RING finger domain
zf-C3HC4_2	PF13923	141 - 181	6.1e-07	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	141 - 181	1.5e-06	Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX	PF13445	141 - 179	3.7e-06	RING-type zinc-finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007795)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G44410
fragaria_vesca	FvH4_7g00150 FvH4_7g00150 FvH4_7g00150
malus_domestica	MD02G1318800.v1.1 MD07G1002400.v1.1
prunus_persica	Prupe.2G003800_v2.0.a1 Prupe.2G003800_v2.0.a1 Prupe.2G003800_v2.0.a1
pyrus_communis	pycom02g26710 pycom07g00240
rosa_chinensis	RchiOBHm_Chr1g0313401
rosa_laevigata	RLG00000030800
rosa_multiflora	Rmu_sc0000035.1_g000030 Rmu_sc0000035.1_g000044
rosa_roxburghii	Rroxscaffold_4G00332220
rosa_rugosa	RorugPtG0004700.1
rosa_samantha	Rh1AG003200 Rh1BG000200 Rh1CG001400 Rh1CG007600 Rh1DG002700 Rh1DG003100
rosa_wichuraiana	Rw1G000200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1163
AarI	CACCTGC	1 cut(s)	862
AbsI	CCTCGAGG	1 cut(s)	995
Acc36I	ACCTGC	1 cut(s)	862
AciI	CCGC	4 cut(s)	468, 512, 691, 924
AclWI	GGATC	2 cut(s)	443, 1194
AcsI	RAATTY	2 cut(s)	207, 1210
AcuI	CTGAAG	1 cut(s)	912
AfiI	CCNNNNNNNGG	4 cut(s)	177, 404, 581, 642
AflIII	ACRYGT	1 cut(s)	461
AhlI	ACTAGT	1 cut(s)	812
AjnI	CCWGG	2 cut(s)	170, 828
AjuI	GAANNNNNNNTTGG	2 cut(s)	947, 979
AluBI	AGCT	4 cut(s)	112, 203, 296, 411
AluI	AGCT	4 cut(s)	112, 203, 296, 411
Alw26I	GTCTC	1 cut(s)	857
AlwI	GGATC	2 cut(s)	443, 1194
Ama87I	CYCGRG	2 cut(s)	300, 995
AoxI	GGCC	4 cut(s)	485, 633, 644, 694
ApaI	GGGCCC	1 cut(s)	698
ApeKI	GCWGC	6 cut(s)	181, 296, 676, 679, 1082, 1121
ApoI	RAATTY	2 cut(s)	207, 1210
Asp700I	GAANNNNTTC	2 cut(s)	723, 1246
AspA2I	CCTAGG	1 cut(s)	641
AspLEI	GCGC	1 cut(s)	1144
AspS9I	GGNCC	6 cut(s)	634, 645, 694, 695, 832, 991
AsuHPI	GGTGA	4 cut(s)	94, 347, 843, 1007
AvaI	CYCGRG	2 cut(s)	300, 995
AvaII	GGWCC	2 cut(s)	832, 991
AvrII	CCTAGG	1 cut(s)	641
BaeGI	GKGCMC	2 cut(s)	698, 825
BaeI	ACNNNNGTAYC	4 cut(s)	366, 399, 1056, 1089
BanII	GRGCYC	1 cut(s)	698
BbsI	GAAGAC	1 cut(s)	772
BbvI	GCAGC	6 cut(s)	193, 283, 688, 691, 1069, 1108
BccI	CCATC	3 cut(s)	364, 587, 706
BciT130I	CCWGG	2 cut(s)	172, 830
BciVI	GTATCC	1 cut(s)	367
BcoDI	GTCTC	1 cut(s)	857
BcuI	ACTAGT	1 cut(s)	812
BfaI	CTAG	6 cut(s)	155, 177, 642, 813, 1236, 1290
BfmI	CTRYAG	1 cut(s)	1083
BfuAI	ACCTGC	1 cut(s)	862
BfuI	GTATCC	1 cut(s)	367
BisI	GCNGC	7 cut(s)	182, 297, 677, 680, 924, 1083, 1122
BlnI	CCTAGG	1 cut(s)	641
BlsI	GCNGC	7 cut(s)	183, 298, 678, 681, 925, 1084, 1123
Bme1390I	CCNGG	2 cut(s)	172, 830
Bme18I	GGWCC	2 cut(s)	832, 991
BmeT110I	CYCGRG	2 cut(s)	300, 995
BmgT120I	GGNCC	6 cut(s)	634, 645, 694, 695, 832, 991
BmiI	GGNNCC	5 cut(s)	636, 647, 695, 696, 922
BmrFI	CCNGG	2 cut(s)	172, 830
BmrI	ACTGGG	2 cut(s)	374, 414
BmsI	GCATC	4 cut(s)	277, 857, 1129, 1231
BmuI	ACTGGG	2 cut(s)	374, 414
BpiI	GAAGAC	1 cut(s)	772
BpmI	CTGGAG	1 cut(s)	276
BsaBI	GATNNNNATC	1 cut(s)	1279
BsaJI	CCNNGG	4 cut(s)	124, 171, 357, 641
Bsc4I	CCNNNNNNNGG	4 cut(s)	177, 404, 581, 642
Bse1I	ACTGG	2 cut(s)	369, 409
Bse3DI	GCAATG	2 cut(s)	1077, 1217
Bse8I	GATNNNNATC	1 cut(s)	1279
BseBI	CCWGG	2 cut(s)	172, 830
BseDI	CCNNGG	4 cut(s)	124, 171, 357, 641
BseGI	GGATG	3 cut(s)	375, 598, 1222
BseJI	GATNNNNATC	1 cut(s)	1279
BseLI	CCNNNNNNNGG	4 cut(s)	177, 404, 581, 642
BseMI	GCAATG	2 cut(s)	1077, 1217
BseMII	CTCAG	1 cut(s)	852
BseNI	ACTGG	2 cut(s)	369, 409
BseSI	GKGCMC	2 cut(s)	698, 825
BseXI	GCAGC	6 cut(s)	193, 283, 688, 691, 1069, 1108
BseYI	CCCAGC	1 cut(s)	824
BsgI	GTGCAG	1 cut(s)	636
BshFI	GGCC	4 cut(s)	487, 635, 646, 696
BsiHKCI	CYCGRG	2 cut(s)	300, 995
BslI	CCNNNNNNNGG	4 cut(s)	177, 404, 581, 642
BsmAI	GTCTC	1 cut(s)	857
BsmI	GAATGC	1 cut(s)	536
BsnI	GGCC	4 cut(s)	487, 635, 646, 696
BsoBI	CYCGRG	2 cut(s)	300, 995
Bsp120I	GGGCCC	1 cut(s)	694
Bsp1286I	GDGCHC	2 cut(s)	698, 825
Bsp143I	GATC	3 cut(s)	84, 448, 1186
Bsp19I	CCATGG	1 cut(s)	124
BspACI	CCGC	4 cut(s)	468, 512, 691, 924
BspANI	GGCC	4 cut(s)	487, 635, 646, 696
BspCNI	CTCAG	1 cut(s)	853
BspHI	TCATGA	1 cut(s)	33
BspLI	GGNNCC	5 cut(s)	636, 647, 695, 696, 922
BspMAI	CTGCAG	1 cut(s)	1087
BspMI	ACCTGC	1 cut(s)	862
BspPI	GGATC	2 cut(s)	443, 1194
BsrDI	GCAATG	2 cut(s)	1077, 1217
BsrI	ACTGG	2 cut(s)	369, 409
BssECI	CCNNGG	4 cut(s)	124, 171, 357, 641
BssMI	GATC	3 cut(s)	84, 448, 1186
BssT1I	CCWWGG	3 cut(s)	124, 357, 641
Bst2UI	CCWGG	2 cut(s)	172, 830
Bst4CI	ACNGT	4 cut(s)	272, 310, 771, 1034
BstAPI	GCANNNNNTGC	1 cut(s)	854
BstDEI	CTNAG	2 cut(s)	734, 861
BstDSI	CCRYGG	1 cut(s)	124
BstEII	GGTNACC	1 cut(s)	1013
BstF5I	GGATG	3 cut(s)	375, 598, 1222
BstHHI	GCGC	1 cut(s)	1144
BstKTI	GATC	3 cut(s)	87, 451, 1189
BstMAI	GTCTC	1 cut(s)	857
BstMBI	GATC	3 cut(s)	84, 448, 1186
BstMWI	GCNNNNNNNGC	3 cut(s)	296, 688, 854
BstNI	CCWGG	2 cut(s)	172, 830
BstNSI	RCATGY	2 cut(s)	346, 465
BstPI	GGTNACC	1 cut(s)	1013
BstSCI	CCNGG	2 cut(s)	170, 828
BstSFI	CTRYAG	1 cut(s)	1083
BstSLI	GKGCMC	2 cut(s)	698, 825
BstV1I	GCAGC	6 cut(s)	193, 283, 688, 691, 1069, 1108
BstV2I	GAAGAC	1 cut(s)	772
BstX2I	RGATCY	1 cut(s)	448
BstXI	CCANNNNNNTGG	2 cut(s)	364, 1183
BstYI	RGATCY	1 cut(s)	448
BsuI	GTATCC	1 cut(s)	367
BsuRI	GGCC	4 cut(s)	487, 635, 646, 696
BtgI	CCRYGG	1 cut(s)	124
BtsCI	GGATG	3 cut(s)	375, 598, 1222
BtsI	GCAGTG	1 cut(s)	231
BtsIMutI	CAGTG	4 cut(s)	231, 315, 362, 858
BveI	ACCTGC	1 cut(s)	862
CciI	TCATGA	1 cut(s)	33
CfoI	GCGC	1 cut(s)	1144
Cfr13I	GGNCC	6 cut(s)	634, 645, 694, 695, 832, 991
CseI	GACGC	2 cut(s)	138, 173
CviAII	CATG	8 cut(s)	34, 125, 343, 462, 788, 799, 884, 1019
DdeI	CTNAG	2 cut(s)	734, 861
DpnI	GATC	3 cut(s)	86, 450, 1188
DpnII	GATC	3 cut(s)	84, 448, 1186
DraI	TTTAAA	1 cut(s)	48
Eco130I	CCWWGG	3 cut(s)	124, 357, 641
Eco24I	GRGCYC	1 cut(s)	698
Eco47I	GGWCC	2 cut(s)	832, 991
Eco57I	CTGAAG	1 cut(s)	912
Eco88I	CYCGRG	2 cut(s)	300, 995
Eco91I	GGTNACC	1 cut(s)	1013
EcoO109I	RGGNCCY	4 cut(s)	634, 645, 694, 991
EcoO65I	GGTNACC	1 cut(s)	1013
EcoRII	CCWGG	2 cut(s)	170, 828
EcoT14I	CCWWGG	3 cut(s)	124, 357, 641
EcoT22I	ATGCAT	1 cut(s)	348
EcoT38I	GRGCYC	1 cut(s)	698
ErhI	CCWWGG	3 cut(s)	124, 357, 641
FaeI	CATG	8 cut(s)	37, 128, 346, 465, 791, 802, 887, 1022
FatI	CATG	8 cut(s)	33, 124, 342, 461, 787, 798, 883, 1018
FauI	CCCGC	1 cut(s)	684
Fnu4HI	GCNGC	7 cut(s)	182, 297, 677, 680, 924, 1083, 1122
FokI	GGATG	3 cut(s)	382, 605, 1209
FriOI	GRGCYC	1 cut(s)	698
Fsp4HI	GCNGC	7 cut(s)	182, 297, 677, 680, 924, 1083, 1122
FspBI	CTAG	6 cut(s)	155, 177, 642, 813, 1236, 1290
GlaI	GCGC	1 cut(s)	1143
GluI	GCNGC	7 cut(s)	182, 297, 677, 680, 924, 1083, 1122
GsaI	CCCAGC	1 cut(s)	828
GsuI	CTGGAG	1 cut(s)	276
HaeIII	GGCC	4 cut(s)	487, 635, 646, 696
HgaI	GACGC	2 cut(s)	138, 173
HhaI	GCGC	1 cut(s)	1144
Hin1II	CATG	8 cut(s)	37, 128, 346, 465, 791, 802, 887, 1022
Hin6I	GCGC	1 cut(s)	1142
HinP1I	GCGC	1 cut(s)	1142
HinfI	GANTC	9 cut(s)	77, 314, 515, 571, 620, 724, 887, 968, 1262
HphI	GGTGA	4 cut(s)	94, 347, 843, 1007
Hpy188III	TCNNGA	3 cut(s)	34, 903, 1251
HpyAV	CCTTC	3 cut(s)	902, 1287, 1293
HpyCH4III	ACNGT	4 cut(s)	272, 310, 771, 1034
HpyCH4IV	ACGT	1 cut(s)	142
HpyCH4V	TGCA	8 cut(s)	346, 524, 653, 791, 962, 1085, 1217, 1222
HpyF10VI	GCNNNNNNNGC	3 cut(s)	296, 688, 854
HpyF3I	CTNAG	2 cut(s)	734, 861
HpySE526I	ACGT	1 cut(s)	142
Hsp92II	CATG	8 cut(s)	37, 128, 346, 465, 791, 802, 887, 1022
HspAI	GCGC	1 cut(s)	1142
Kzo9I	GATC	3 cut(s)	84, 448, 1186
LmnI	GCTCC	4 cut(s)	295, 408, 920, 1149
Lsp1109I	GCAGC	6 cut(s)	193, 283, 688, 691, 1069, 1108
LweI	GCATC	4 cut(s)	277, 857, 1129, 1231
MaeI	CTAG	6 cut(s)	155, 177, 642, 813, 1236, 1290
MaeII	ACGT	1 cut(s)	142
MaeIII	GTNAC	4 cut(s)	163, 553, 771, 1013
MalI	GATC	3 cut(s)	86, 450, 1188
MboI	GATC	3 cut(s)	84, 448, 1186
MboII	GAAGA	8 cut(s)	38, 149, 693, 728, 777, 927, 1038, 1224
MflI	RGATCY	1 cut(s)	448
MhlI	GDGCHC	2 cut(s)	698, 825
MluCI	AATT	5 cut(s)	207, 219, 576, 1210, 1246
MlyI	GAGTC	1 cut(s)	629
Mph1103I	ATGCAT	1 cut(s)	348
MroXI	GAANNNNTTC	2 cut(s)	723, 1246
MseI	TTAA	1 cut(s)	47
MslI	CAYNNNNRTG	2 cut(s)	882, 1181
MspA1I	CMGCKG	1 cut(s)	296
MspR9I	CCNGG	2 cut(s)	172, 830
Mva1269I	GAATGC	1 cut(s)	536
MvaI	CCWGG	2 cut(s)	172, 830
MwoI	GCNNNNNNNGC	3 cut(s)	296, 688, 854
NcoI	CCATGG	1 cut(s)	124
NdeII	GATC	3 cut(s)	84, 448, 1186
NlaIII	CATG	8 cut(s)	37, 128, 346, 465, 791, 802, 887, 1022
NlaIV	GGNNCC	5 cut(s)	636, 647, 695, 696, 922
NmuCI	GTSAC	3 cut(s)	163, 771, 1013
NsiI	ATGCAT	1 cut(s)	348
NspI	RCATGY	2 cut(s)	346, 465
PaeR7I	CTCGAG	1 cut(s)	995
PagI	TCATGA	1 cut(s)	33
PaqCI	CACCTGC	1 cut(s)	862
PciI	ACATGT	1 cut(s)	461
PcsI	WCGNNNNNNNCGW	1 cut(s)	993
PctI	GAATGC	1 cut(s)	536
PdmI	GAANNNNTTC	2 cut(s)	723, 1246
PfeI	GAWTC	8 cut(s)	77, 314, 515, 571, 724, 887, 968, 1262
PkrI	GCNGC	7 cut(s)	183, 298, 678, 681, 925, 1084, 1123
PleI	GAGTC	1 cut(s)	628
PpsI	GAGTC	1 cut(s)	628
PpuMI	RGGWCCY	1 cut(s)	991
PscI	ACATGT	1 cut(s)	461
PsiI	TTATAA	1 cut(s)	1163
Psp5II	RGGWCCY	1 cut(s)	991
Psp6I	CCWGG	2 cut(s)	170, 828
PspEI	GGTNACC	1 cut(s)	1013
PspFI	CCCAGC	1 cut(s)	824
PspGI	CCWGG	2 cut(s)	170, 828
PspN4I	GGNNCC	5 cut(s)	636, 647, 695, 696, 922
PspOMI	GGGCCC	1 cut(s)	694
PspPI	GGNCC	6 cut(s)	634, 645, 694, 695, 832, 991
PspPPI	RGGWCCY	1 cut(s)	991
PspXI	VCTCGAGB	1 cut(s)	995
PstI	CTGCAG	1 cut(s)	1087
PsuI	RGATCY	1 cut(s)	448
PvuII	CAGCTG	1 cut(s)	296
RseI	CAYNNNNRTG	2 cut(s)	882, 1181
SaqAI	TTAA	1 cut(s)	47
SatI	GCNGC	7 cut(s)	182, 297, 677, 680, 924, 1083, 1122
Sau3AI	GATC	3 cut(s)	84, 448, 1186
Sau96I	GGNCC	6 cut(s)	634, 645, 694, 695, 832, 991
SchI	GAGTC	1 cut(s)	629
ScrFI	CCNGG	2 cut(s)	172, 830
SduI	GDGCHC	2 cut(s)	698, 825
SfaNI	GCATC	4 cut(s)	277, 857, 1129, 1231
SfcI	CTRYAG	1 cut(s)	1083
Sfr274I	CTCGAG	1 cut(s)	995
SinI	GGWCC	2 cut(s)	832, 991
SlaI	CTCGAG	1 cut(s)	995
SmiMI	CAYNNNNRTG	2 cut(s)	882, 1181
SmlI	CTYRAG	1 cut(s)	995
SmoI	CTYRAG	1 cut(s)	995
SpeI	ACTAGT	1 cut(s)	812
Sse9I	AATT	5 cut(s)	207, 219, 576, 1210, 1246
SsiI	CCGC	4 cut(s)	468, 512, 691, 924
SspMI	CTAG	6 cut(s)	155, 177, 642, 813, 1236, 1290
StyD4I	CCNGG	2 cut(s)	170, 828
StyI	CCWWGG	3 cut(s)	124, 357, 641
TaaI	ACNGT	4 cut(s)	272, 310, 771, 1034
TaiI	ACGT	1 cut(s)	145
TaqI	TCGA	3 cut(s)	105, 987, 996
TasI	AATT	5 cut(s)	207, 219, 576, 1210, 1246
TauI	GCSGC	1 cut(s)	926
TfiI	GAWTC	8 cut(s)	77, 314, 515, 571, 724, 887, 968, 1262
Tru1I	TTAA	1 cut(s)	47
Tru9I	TTAA	1 cut(s)	47
TscAI	CASTG	4 cut(s)	238, 315, 369, 865
TseFI	GTSAC	3 cut(s)	163, 771, 1013
TseI	GCWGC	6 cut(s)	181, 296, 676, 679, 1082, 1121
Tsp45I	GTSAC	3 cut(s)	163, 771, 1013
TspDTI	ATGAA	1 cut(s)	933
TspRI	CASTG	4 cut(s)	238, 315, 369, 865
VpaK11BI	GGWCC	2 cut(s)	832, 991
XapI	RAATTY	2 cut(s)	207, 1210
XceI	RCATGY	2 cut(s)	346, 465
XhoI	CTCGAG	1 cut(s)	995
XmaJI	CCTAGG	1 cut(s)	641
XmnI	GAANNNNTTC	2 cut(s)	723, 1246
XspI	CTAG	6 cut(s)	155, 177, 642, 813, 1236, 1290
Zsp2I	ATGCAT	1 cut(s)	348

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.