Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0005654

Physical Location & Seq

Genomic Coordinates Reverse (-)

37371108 .. 37378360

Gene Structure 7253 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1319300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1422 bp

ATGGTGTTCTTCAGGAGCGTCACCGCGCTTTCCAAGCTCCGTTCCCGTCTTGGGCAACAGTCGAGTCTCAGAGATTCCGTCAGATGGATCCAAACGCAGAGCTCCACTGATCTTGAACTTCGTTCTCAGTTGGCTGAATTGATTCCTGAACAACAGGAACGTCTGAAGAAACTCAAGGCAGATTATGGAAAAGTTCAACTGGGCAATATCACTGTTGATATGGTGATTGGTGGAATGAGAGGAATGACAGGGTTGCTGTGGGAAACCTCCTTACTTGATCCAGATGAGGGAATTCGCTTCAGGGGTTTGTCAATTCCAGAATGCCAGAAAGTATTACCAGCTGCAAAGCCAGGTGGAGAACCCTTGCCTGAGGGTCTTCTGTGGCTGCTTTTAACAGGAAAGGTACCTAGCAAAGAGCAAGTAGATGCATTATCCAAGGAATTGAGGACTCGTGCCGTAGTTCCAGATTATGTGTATAAGGCCATTGATGCTCTGCCTATTACAGCACATCCAATGACCCAGTTTACCACTGGTGTCATGGCCCTCCAGGTAGAGAGTGAATTCCAGAAGGCATATGAAAAGGGGATACATAAATCAAAGTACTGGGAGCCAACTTTTGAGGATTCACTTAGCTTGATTGCACGAGTGCCAGTAGTAGCTGCCTATATTTATCAAAGAATTTTCAAGGATGGAAAAGTAAAACAGATTGATGATTCTTTGGATTATGGTGCAAATTTTTCACACATGCTGGGTTTTGACGATCCCAAGGTGTATGAGCTCATGAGGCTTTATGTCACCATCCATAGTGATCATGAGGGTGGGAATGTCAGTGCTCACACGGGACACTTGGTTGCTAGTGCACTTTCAGATCCGTATCTTTCATTTGCAGCTGCTTTAAATGGCTTGGCTGGCCCACTCCATGGTTTGGCTAATCAGGAAGTATTGCTTTGGATAAAATCTGTGGTAGATGAAGTTGGAGAGAATGCAACTACACAGCAGTTGAAAGATTATGTCTGGCAAACGTTAAAAAGTGGGAAGGTTGTTCCTGGTTTTGGACATGGAGTCTTGCGTAAAACAGACCCACGATACACATGTCAAAGGGAGTTTGCATTGAAACACATGCCTGATGATCCACTGTTTCAGTTGGTCTCCAAGCTTTATGAAGTTGTGCCTCCCATACTTACCGAACTAGGCAAGGTTAAAAACCCATGGCCCAATGTAGATGCACACAGTGGAGTTCTGTTGAACCATTTTGGTTTGACTGAGGCAAGATATTTTACTGTTCTCTTTGGTGTATCGAGGAGTATAGGGATTGGTTCTCAGTTGATATGGGACCGAGCTCTTGGTTTGCCTCTCGAAAGGCCAAAAAGTGTAACGATGGAATCGCTTGAGAATTTCTGCAAGAAAGCAGCTTCGTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004108 GO:0005488 GO:0005524 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0008144 GO:0008150 GO:0008152 GO:0008270 GO:0009060 GO:0009507 GO:0009536 GO:0009987 GO:0010035 GO:0010038 GO:0015980 GO:0016740 GO:0016746 GO:0016999 GO:0017076 GO:0017144 GO:0019752 GO:0030312 GO:0030554 GO:0031974 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036440 GO:0042221 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045333 GO:0046686 GO:0046872 GO:0046912 GO:0046914 GO:0050896 GO:0055114 GO:0070013 GO:0071704 GO:0071944 GO:0072350 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00020 ko00630 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Citrate_synt

Protein Analysis

474

Amino Acids

52.65

Weight (kDa)

7.21

Isoelectric Point (pI)

28.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Citrate_synt	PF00285	78 - 456	4e-95	Citrate synthase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005654)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G44350 AT2G44350 AT2G44350 AT2G44350 AT3G60100 AT3G60100 AT3G60100
fragaria_vesca	FvH4_7g00080 FvH4_7g00080 FvH4_7g00080
malus_domestica	MD00G1055300.v1.1 MD02G1319300.v1.1
prunus_persica	Prupe.2G002900_v2.0.a1 Prupe.2G002900_v2.0.a1
pyrus_communis	pycom07g00060
rosa_chinensis	RchiOBHm_Chr1g0313271
rosa_laevigata	RLG00000030810
rosa_multiflora	Rmu_sc0000035.1_g000001 Rmu_sc0004761.1_g000015
rosa_roxburghii	Rroxscaffold_4G00332290
rosa_rugosa	RorugPtG0002200 RorugPtG0002300.1 RorugPtG0002400.1
rosa_samantha	Rh1AG002300 Rh1CG004400 Rh1CG006800 Rh1DG002200
rosa_wichuraiana	Rw1G000130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	403
AccB1I	GGYRCC	1 cut(s)	403
AccB7I	CCANNNNNTGG	2 cut(s)	920, 925
AccII	CGCG	1 cut(s)	26
AciI	CCGC	1 cut(s)	24
AclI	AACGTT	1 cut(s)	1022
AclWI	GGATC	6 cut(s)	82, 95, 272, 755, 863, 1124
AcsI	RAATTY	5 cut(s)	291, 560, 678, 733, 1393
AcuI	CTGAAG	2 cut(s)	185, 283
AdeI	CACNNNGTG	1 cut(s)	1232
AfaI	GTAC	2 cut(s)	405, 602
AfiI	CCNNNNNNNGG	6 cut(s)	51, 84, 287, 920, 925, 1052
AflIII	ACRYGT	1 cut(s)	1091
AgsI	TTSAA	6 cut(s)	116, 197, 685, 1003, 1114, 1246
AhdI	GACNNNNNGTC	1 cut(s)	1061
AjnI	CCWGG	3 cut(s)	349, 546, 1045
Alw21I	GWGCWC	5 cut(s)	104, 780, 835, 862, 1342
Alw26I	GTCTC	2 cut(s)	71, 1153
Alw44I	GTGCAC	1 cut(s)	858
AlwI	GGATC	6 cut(s)	82, 95, 272, 755, 863, 1124
AoxI	GGCC	5 cut(s)	480, 540, 910, 1211, 1361
ApaLI	GTGCAC	1 cut(s)	858
ApeKI	GCWGC	6 cut(s)	341, 385, 659, 887, 890, 1409
ApoI	RAATTY	5 cut(s)	291, 560, 678, 733, 1393
Asp700I	GAANNNNTTC	1 cut(s)	141
Asp718I	GGTACC	1 cut(s)	403
AspLEI	GCGC	1 cut(s)	28
AspS9I	GGNCC	4 cut(s)	541, 911, 1212, 1333
AsuHPI	GGTGA	3 cut(s)	13, 235, 787
AvaII	GGWCC	1 cut(s)	1333
AxyI	CCTNAGG	1 cut(s)	369
BaeGI	GKGCMC	1 cut(s)	862
BaeI	ACNNNNGTAYC	2 cut(s)	1078, 1111
BamHI	GGATCC	1 cut(s)	87
BanI	GGYRCC	1 cut(s)	403
BanII	GRGCYC	3 cut(s)	104, 780, 1342
BauI	CACGAG	2 cut(s)	450, 642
BbsI	GAAGAC	1 cut(s)	368
Bbv12I	GWGCWC	5 cut(s)	104, 780, 835, 862, 1342
BbvI	GCAGC	5 cut(s)	328, 372, 646, 877, 899
BccI	CCATC	4 cut(s)	78, 683, 806, 1372
BceAI	ACGGC	1 cut(s)	440
BciT130I	CCWGG	3 cut(s)	351, 548, 1047
BciVI	GTATCC	1 cut(s)	579
BclI	TGATCA	1 cut(s)	808
BcoDI	GTCTC	2 cut(s)	71, 1153
BfaI	CTAG	3 cut(s)	408, 855, 1190
BfuI	GTATCC	1 cut(s)	579
BisI	GCNGC	6 cut(s)	342, 386, 660, 888, 891, 1410
BlsI	GCNGC	6 cut(s)	343, 387, 661, 889, 892, 1411
BmcAI	AGTACT	1 cut(s)	602
Bme1390I	CCNGG	3 cut(s)	351, 548, 1047
Bme18I	GGWCC	1 cut(s)	1333
BmeRI	GACNNNNNGTC	1 cut(s)	1061
BmgT120I	GGNCC	4 cut(s)	541, 911, 1212, 1333
BmiI	GGNNCC	4 cut(s)	89, 405, 609, 1334
BmrFI	CCNGG	3 cut(s)	351, 548, 1047
BmrI	ACTGGG	3 cut(s)	209, 514, 613
BmsI	GCATC	3 cut(s)	415, 478, 1213
BmuI	ACTGGG	3 cut(s)	209, 514, 613
BpiI	GAAGAC	1 cut(s)	368
BpmI	CTGGAG	1 cut(s)	530
BpuEI	CTTGAG	2 cut(s)	158, 1409
BsaBI	GATNNNNATC	1 cut(s)	873
BsaI	GGTCTC	1 cut(s)	1153
BsaJI	CCNNGG	4 cut(s)	435, 765, 919, 1208
Bsc4I	CCNNNNNNNGG	6 cut(s)	51, 84, 287, 920, 925, 1052
Bse1I	ACTGG	5 cut(s)	204, 520, 535, 608, 650
Bse21I	CCTNAGG	1 cut(s)	369
Bse8I	GATNNNNATC	1 cut(s)	873
BseBI	CCWGG	3 cut(s)	351, 548, 1047
BseDI	CCNNGG	4 cut(s)	435, 765, 919, 1208
BseGI	GGATG	3 cut(s)	508, 694, 798
BseJI	GATNNNNATC	1 cut(s)	873
BseLI	CCNNNNNNNGG	6 cut(s)	51, 84, 287, 920, 925, 1052
BseMII	CTCAG	5 cut(s)	82, 140, 360, 1254, 1334
BseNI	ACTGG	5 cut(s)	204, 520, 535, 608, 650
BseRI	GAGGAG	1 cut(s)	1315
BseSI	GKGCMC	1 cut(s)	862
BseXI	GCAGC	5 cut(s)	328, 372, 646, 877, 899
BseYI	CCCAGC	1 cut(s)	748
Bsh1236I	CGCG	1 cut(s)	26
BshFI	GGCC	5 cut(s)	482, 542, 912, 1213, 1363
BshNI	GGYRCC	1 cut(s)	403
BsiHKAI	GWGCWC	5 cut(s)	104, 780, 835, 862, 1342
BslFI	GGGAC	2 cut(s)	855, 1346
BslI	CCNNNNNNNGG	6 cut(s)	51, 84, 287, 920, 925, 1052
BsmAI	GTCTC	2 cut(s)	71, 1153
BsmFI	GGGAC	2 cut(s)	855, 1346
BsmI	GAATGC	2 cut(s)	326, 988
BsnI	GGCC	5 cut(s)	482, 542, 912, 1213, 1363
Bso31I	GGTCTC	1 cut(s)	1153
Bsp1286I	GDGCHC	5 cut(s)	104, 780, 835, 862, 1342
Bsp143I	GATC	7 cut(s)	87, 109, 277, 760, 808, 868, 1129
Bsp19I	CCATGG	2 cut(s)	919, 1208
BspACI	CCGC	1 cut(s)	24
BspANI	GGCC	5 cut(s)	482, 542, 912, 1213, 1363
BspCNI	CTCAG	5 cut(s)	81, 139, 361, 1255, 1333
BspFNI	CGCG	1 cut(s)	26
BspHI	TCATGA	2 cut(s)	780, 811
BspLI	GGNNCC	4 cut(s)	89, 405, 609, 1334
BspPI	GGATC	6 cut(s)	82, 95, 272, 755, 863, 1124
BspT107I	GGYRCC	1 cut(s)	403
BspTNI	GGTCTC	1 cut(s)	1153
BsrI	ACTGG	5 cut(s)	204, 520, 535, 608, 650
BssECI	CCNNGG	4 cut(s)	435, 765, 919, 1208
BssMI	GATC	7 cut(s)	87, 109, 277, 760, 808, 868, 1129
BssSI	CACGAG	2 cut(s)	450, 642
BssT1I	CCWWGG	4 cut(s)	435, 765, 919, 1208
Bst2BI	CACGAG	2 cut(s)	450, 642
Bst2UI	CCWGG	3 cut(s)	351, 548, 1047
Bst4CI	ACNGT	5 cut(s)	60, 214, 1137, 1232, 1282
BstC8I	GCNNGC	1 cut(s)	910
BstDEI	CTNAG	6 cut(s)	68, 126, 369, 629, 1263, 1320
BstDSI	CCRYGG	2 cut(s)	919, 1208
BstF5I	GGATG	3 cut(s)	508, 694, 798
BstFNI	CGCG	1 cut(s)	26
BstHHI	GCGC	1 cut(s)	28
BstKTI	GATC	7 cut(s)	90, 112, 280, 763, 811, 871, 1132
BstMAI	GTCTC	2 cut(s)	71, 1153
BstMBI	GATC	7 cut(s)	87, 109, 277, 760, 808, 868, 1129
BstMWI	GCNNNNNNNGC	4 cut(s)	34, 488, 784, 909
BstNI	CCWGG	3 cut(s)	351, 548, 1047
BstNSI	RCATGY	3 cut(s)	748, 1095, 1123
BstSCI	CCNGG	3 cut(s)	349, 546, 1045
BstSLI	GKGCMC	1 cut(s)	862
BstUI	CGCG	1 cut(s)	26
BstV1I	GCAGC	5 cut(s)	328, 372, 646, 877, 899
BstV2I	GAAGAC	1 cut(s)	368
BstX2I	RGATCY	2 cut(s)	87, 868
BstYI	RGATCY	2 cut(s)	87, 868
Bsu36I	CCTNAGG	1 cut(s)	369
BsuI	GTATCC	1 cut(s)	579
BsuRI	GGCC	5 cut(s)	482, 542, 912, 1213, 1363
BtgI	CCRYGG	2 cut(s)	919, 1208
BtsCI	GGATG	3 cut(s)	508, 694, 798
BtsIMutI	CAGTG	6 cut(s)	105, 210, 528, 835, 1133, 1237
Cac8I	GCNNGC	1 cut(s)	910
CciI	TCATGA	2 cut(s)	780, 811
CfoI	GCGC	1 cut(s)	28
Cfr13I	GGNCC	4 cut(s)	541, 911, 1212, 1333
CseI	GACGC	1 cut(s)	7
Csp6I	GTAC	2 cut(s)	404, 601
CspCI	CAANNNNNGTGG	2 cut(s)	94, 129
CviAII	CATG	9 cut(s)	538, 745, 781, 812, 920, 1058, 1092, 1120, 1209
CviQI	GTAC	2 cut(s)	404, 601
DdeI	CTNAG	6 cut(s)	68, 126, 369, 629, 1263, 1320
DpnI	GATC	7 cut(s)	89, 111, 279, 762, 810, 870, 1131
DpnII	GATC	7 cut(s)	87, 109, 277, 760, 808, 868, 1129
DraI	TTTAAA	1 cut(s)	897
DraIII	CACNNNGTG	1 cut(s)	1232
DriI	GACNNNNNGTC	1 cut(s)	1061
Eam1105I	GACNNNNNGTC	1 cut(s)	1061
Ecl136II	GAGCTC	3 cut(s)	102, 778, 1340
Eco130I	CCWWGG	4 cut(s)	435, 765, 919, 1208
Eco24I	GRGCYC	3 cut(s)	104, 780, 1342
Eco31I	GGTCTC	1 cut(s)	1153
Eco47I	GGWCC	1 cut(s)	1333
Eco53kI	GAGCTC	3 cut(s)	102, 778, 1340
Eco57I	CTGAAG	2 cut(s)	185, 283
Eco81I	CCTNAGG	1 cut(s)	369
EcoICRI	GAGCTC	3 cut(s)	102, 778, 1340
EcoRI	GAATTC	2 cut(s)	291, 560
EcoRII	CCWGG	3 cut(s)	349, 546, 1045
EcoT14I	CCWWGG	4 cut(s)	435, 765, 919, 1208
EcoT22I	ATGCAT	1 cut(s)	430
EcoT38I	GRGCYC	3 cut(s)	104, 780, 1342
ErhI	CCWWGG	4 cut(s)	435, 765, 919, 1208
FaeI	CATG	9 cut(s)	541, 748, 784, 815, 923, 1061, 1095, 1123, 1212
FalI	AAGNNNNNCTT	2 cut(s)	930, 962
FaqI	GGGAC	2 cut(s)	855, 1346
FatI	CATG	9 cut(s)	537, 744, 780, 811, 919, 1057, 1091, 1119, 1208
FauNDI	CATATG	1 cut(s)	574
FbaI	TGATCA	1 cut(s)	808
Fnu4HI	GCNGC	6 cut(s)	342, 386, 660, 888, 891, 1410
FokI	GGATG	3 cut(s)	495, 701, 785
FriOI	GRGCYC	3 cut(s)	104, 780, 1342
Fsp4HI	GCNGC	6 cut(s)	342, 386, 660, 888, 891, 1410
FspBI	CTAG	3 cut(s)	408, 855, 1190
GlaI	GCGC	1 cut(s)	27
GluI	GCNGC	6 cut(s)	342, 386, 660, 888, 891, 1410
GsaI	CCCAGC	1 cut(s)	752
GsuI	CTGGAG	1 cut(s)	530
HaeIII	GGCC	5 cut(s)	482, 542, 912, 1213, 1363
HgaI	GACGC	1 cut(s)	7
HhaI	GCGC	1 cut(s)	28
Hin1II	CATG	9 cut(s)	541, 748, 784, 815, 923, 1061, 1095, 1123, 1212
Hin6I	GCGC	1 cut(s)	26
HinP1I	GCGC	1 cut(s)	26
HindIII	AAGCTT	1 cut(s)	1154
HinfI	GANTC	8 cut(s)	64, 74, 142, 448, 623, 713, 1062, 1382
HphI	GGTGA	3 cut(s)	13, 235, 787
Hpy166II	GTNNAC	2 cut(s)	525, 860
Hpy188I	TCNGA	4 cut(s)	71, 83, 165, 868
Hpy8I	GTNNAC	2 cut(s)	525, 860
HpyAV	CCTTC	2 cut(s)	562, 1030
HpyCH4III	ACNGT	5 cut(s)	60, 214, 1137, 1232, 1282
HpyCH4IV	ACGT	2 cut(s)	160, 1022
HpyF10VI	GCNNNNNNNGC	4 cut(s)	34, 488, 784, 909
HpyF3I	CTNAG	6 cut(s)	68, 126, 369, 629, 1263, 1320
HpySE526I	ACGT	2 cut(s)	160, 1022
Hsp92II	CATG	9 cut(s)	541, 748, 784, 815, 923, 1061, 1095, 1123, 1212
HspAI	GCGC	1 cut(s)	26
KpnI	GGTACC	1 cut(s)	407
Ksp22I	TGATCA	1 cut(s)	808
Kzo9I	GATC	7 cut(s)	87, 109, 277, 760, 808, 868, 1129
LmnI	GCTCC	4 cut(s)	15, 42, 107, 607
Lsp1109I	GCAGC	5 cut(s)	328, 372, 646, 877, 899
LweI	GCATC	3 cut(s)	415, 478, 1213
MaeI	CTAG	3 cut(s)	408, 855, 1190
MaeII	ACGT	2 cut(s)	160, 1022
MaeIII	GTNAC	3 cut(s)	19, 793, 1372
MalI	GATC	7 cut(s)	89, 111, 279, 762, 810, 870, 1131
MboI	GATC	7 cut(s)	87, 109, 277, 760, 808, 868, 1129
MboII	GAAGA	2 cut(s)	178, 368
MflI	RGATCY	2 cut(s)	87, 868
MhlI	GDGCHC	5 cut(s)	104, 780, 835, 862, 1342
MluCI	AATT	8 cut(s)	137, 291, 312, 440, 560, 678, 733, 1393
MlyI	GAGTC	3 cut(s)	73, 442, 1071
MmeI	TCCRAC	1 cut(s)	955
Mph1103I	ATGCAT	1 cut(s)	430
MroXI	GAANNNNTTC	1 cut(s)	141
MseI	TTAA	4 cut(s)	392, 896, 1025, 1200
MslI	CAYNNNNRTG	1 cut(s)	816
MspA1I	CMGCKG	2 cut(s)	341, 890
MspR9I	CCNGG	3 cut(s)	351, 548, 1047
Mva1269I	GAATGC	2 cut(s)	326, 988
MvaI	CCWGG	3 cut(s)	351, 548, 1047
MvnI	CGCG	1 cut(s)	26
MwoI	GCNNNNNNNGC	4 cut(s)	34, 488, 784, 909
NcoI	CCATGG	2 cut(s)	919, 1208
NdeI	CATATG	1 cut(s)	574
NdeII	GATC	7 cut(s)	87, 109, 277, 760, 808, 868, 1129
NlaIII	CATG	9 cut(s)	541, 748, 784, 815, 923, 1061, 1095, 1123, 1212
NlaIV	GGNNCC	4 cut(s)	89, 405, 609, 1334
NmuCI	GTSAC	2 cut(s)	19, 793
NsiI	ATGCAT	1 cut(s)	430
NspI	RCATGY	3 cut(s)	748, 1095, 1123
PagI	TCATGA	2 cut(s)	780, 811
PciI	ACATGT	1 cut(s)	1091
PctI	GAATGC	2 cut(s)	326, 988
PdmI	GAANNNNTTC	1 cut(s)	141
PfeI	GAWTC	5 cut(s)	74, 142, 623, 713, 1382
PflMI	CCANNNNNTGG	2 cut(s)	920, 925
PkrI	GCNGC	6 cut(s)	343, 387, 661, 889, 892, 1411
PleI	GAGTC	3 cut(s)	72, 442, 1070
PpsI	GAGTC	3 cut(s)	72, 442, 1070
PscI	ACATGT	1 cut(s)	1091
Psp124BI	GAGCTC	3 cut(s)	104, 780, 1342
Psp1406I	AACGTT	1 cut(s)	1022
Psp6I	CCWGG	3 cut(s)	349, 546, 1045
PspFI	CCCAGC	1 cut(s)	748
PspGI	CCWGG	3 cut(s)	349, 546, 1045
PspN4I	GGNNCC	4 cut(s)	89, 405, 609, 1334
PspPI	GGNCC	4 cut(s)	541, 911, 1212, 1333
PsuI	RGATCY	2 cut(s)	87, 868
PvuII	CAGCTG	2 cut(s)	341, 890
RsaI	GTAC	2 cut(s)	405, 602
RsaNI	GTAC	2 cut(s)	404, 601
RseI	CAYNNNNRTG	1 cut(s)	816
SacI	GAGCTC	3 cut(s)	104, 780, 1342
SaqAI	TTAA	4 cut(s)	392, 896, 1025, 1200
SatI	GCNGC	6 cut(s)	342, 386, 660, 888, 891, 1410
Sau3AI	GATC	7 cut(s)	87, 109, 277, 760, 808, 868, 1129
Sau96I	GGNCC	4 cut(s)	541, 911, 1212, 1333
ScaI	AGTACT	1 cut(s)	602
SchI	GAGTC	3 cut(s)	73, 442, 1071
ScrFI	CCNGG	3 cut(s)	351, 548, 1047
SduI	GDGCHC	5 cut(s)	104, 780, 835, 862, 1342
SfaNI	GCATC	3 cut(s)	415, 478, 1213
SinI	GGWCC	1 cut(s)	1333
SmiMI	CAYNNNNRTG	1 cut(s)	816
SmlI	CTYRAG	2 cut(s)	173, 1388
SmoI	CTYRAG	2 cut(s)	173, 1388
Sse9I	AATT	8 cut(s)	137, 291, 312, 440, 560, 678, 733, 1393
SsiI	CCGC	1 cut(s)	24
SspMI	CTAG	3 cut(s)	408, 855, 1190
SstI	GAGCTC	3 cut(s)	104, 780, 1342
StyD4I	CCNGG	3 cut(s)	349, 546, 1045
StyI	CCWWGG	4 cut(s)	435, 765, 919, 1208
TaaI	ACNGT	5 cut(s)	60, 214, 1137, 1232, 1282
TaiI	ACGT	2 cut(s)	163, 1025
TaqI	TCGA	3 cut(s)	62, 1298, 1356
TaqII	GACCGA	1 cut(s)	1350
TasI	AATT	8 cut(s)	137, 291, 312, 440, 560, 678, 733, 1393
TatI	WGTACW	1 cut(s)	600
TfiI	GAWTC	5 cut(s)	74, 142, 623, 713, 1382
Tru1I	TTAA	4 cut(s)	392, 896, 1025, 1200
Tru9I	TTAA	4 cut(s)	392, 896, 1025, 1200
TscAI	CASTG	6 cut(s)	112, 217, 535, 835, 1140, 1237
TseFI	GTSAC	2 cut(s)	19, 793
TseI	GCWGC	6 cut(s)	341, 385, 659, 887, 890, 1409
Tsp45I	GTSAC	2 cut(s)	19, 793
TspDTI	ATGAA	4 cut(s)	591, 870, 984, 1176
TspGWI	ACGGA	3 cut(s)	29, 67, 861
TspRI	CASTG	6 cut(s)	112, 217, 535, 835, 1140, 1237
Van91I	CCANNNNNTGG	2 cut(s)	920, 925
VneI	GTGCAC	1 cut(s)	858
VpaK11BI	GGWCC	1 cut(s)	1333
XapI	RAATTY	5 cut(s)	291, 560, 678, 733, 1393
XceI	RCATGY	3 cut(s)	748, 1095, 1123
XcmI	CCANNNNNNNNNTGG	2 cut(s)	527, 535
XmnI	GAANNNNTTC	1 cut(s)	141
XspI	CTAG	3 cut(s)	408, 855, 1190
ZrmI	AGTACT	1 cut(s)	602
Zsp2I	ATGCAT	1 cut(s)	430

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.