MD03G1024200.v1.1

Cytochrome c-type biogenesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012321
Physical Location & Seq
Reverse (-)
2003364 .. 2004288
925 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1024200.v1.1.491

Sequence Viewer

Length: 825 bp
ATGGCCACTCGCATCGCCCTCCGCCTCAAATCCCACCTCCTCCGCGCCACAACCCCGATCCACCACCACCCCATCAAATATCCACCGTCCATCTTCACCCGACTCTCCAACCCTCATCTCCACCCTCCATCAAATCCCCATCTTCCCTTCACCCTCCGTTTCTTCTCCACCGCCCGCCGCGGCCCAACCCGCCCCAAACCCGTCGACATCGGTGCCCGCGCCCGCCAGCTCCAAACCCGCCGACTCTGGACCTACGCTCTGACCTTCAGCTGCATCGCCGGATTCATTGTCATTGTCCTCAACAGCTTCCAAGACCAGCTCGTCTTCTACGTCACGCCAACCGACGCAATCGAGAAGTACTCCACAAACCCAAGCAAGAGCAAGTTCCGGGTCGGCGGCCTGGTCCTCGAAGGCAGCGTCGTCCAGCCGTCCTCCTCGCCGGAGATGGAGTTCGTCGTCACCGATTTGATGACCGATATCTTGGTCCGGTACAAGGGATCATTGCCCGATTTGTTCCGGGAGGGTCACTCGGTGGTGGTGGAGGGATTTGTGAAGCCTTTCACGGATGAAATTCGGAAGGAGATTTCGACGAAAAGCATATCGGAGAAGGCGAGGACCGGCGAGTGCTATTTCGCCGCCACCGAGGTTTTGGCCAAGCATGACGAGAAGTACATGCCGGGTGAAGTCGCCGCCGCGATTGAGAAGAATAAGAAGAAGCTGGAGGAGGCCGGTGCCGGTGCCGGTGAAGATGGTAAAGATGAAATGGGTTACACTTGGATAGTTCATAGTTGGATGATTGATTCAAAAGGATTGATCTTTAGGTGA

Protein Analysis

275

Amino Acids

30.82

Weight (kDa)

9.41

Isoelectric Point (pI)

43.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CcmE PF03100 108 - 226 2.6e-27 CcmE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 320
AccB1I GGYRCC 3 cut(s) 212, 731, 737
AccI GTMKAC 1 cut(s) 204
AccII CGCG 4 cut(s) 45, 180, 219, 695
AclWI GGATC 2 cut(s) 52, 505
AcoI YGGCCR 2 cut(s) 3, 651
AcsI RAATTY 1 cut(s) 570
AcuI CTGAAG 1 cut(s) 250
AdeI CACNNNGTG 1 cut(s) 532
AfaI GTAC 3 cut(s) 359, 491, 671
AfiI CCNNNNNNNGG 1 cut(s) 493
AgsI TTSAA 1 cut(s) 804
AjnI CCWGG 1 cut(s) 399
AloI GAACNNNNNNTCC 2 cut(s) 434, 466
AluBI AGCT 5 cut(s) 229, 270, 306, 319, 718
AluI AGCT 5 cut(s) 229, 270, 306, 319, 718
AlwI GGATC 2 cut(s) 52, 505
AoxI GGCC 5 cut(s) 3, 181, 397, 651, 726
ApeKI GCWGC 2 cut(s) 270, 414
ApoI RAATTY 1 cut(s) 570
ArsI GACNNNNNNTTYG 2 cut(s) 402, 434
Asp700I GAANNNNTTC 1 cut(s) 557
AspLEI GCGC 2 cut(s) 47, 221
AspS9I GGNCC 5 cut(s) 182, 249, 403, 484, 615
AsuC2I CCSGG 3 cut(s) 389, 518, 678
AsuHPI GGTGA 5 cut(s) 88, 142, 451, 692, 755
AvaII GGWCC 4 cut(s) 249, 403, 484, 615
BaeGI GKGCMC 1 cut(s) 217
BalI TGGCCA 2 cut(s) 5, 653
BanI GGYRCC 3 cut(s) 212, 731, 737
BbsI GAAGAC 1 cut(s) 316
BbvI GCAGC 2 cut(s) 257, 426
BccI CCATC 6 cut(s) 80, 98, 136, 147, 439, 743
BceAI ACGGC 1 cut(s) 412
BcgI CGANNNNNNTGC 2 cut(s) 194, 228
BciT130I CCWGG 1 cut(s) 401
BcnI CCSGG 3 cut(s) 389, 518, 678
BisI GCNGC 8 cut(s) 178, 181, 271, 397, 415, 636, 690, 693
BlsI GCNGC 8 cut(s) 179, 182, 272, 398, 416, 637, 691, 694
BmcAI AGTACT 1 cut(s) 359
Bme1390I CCNGG 4 cut(s) 389, 401, 518, 678
Bme18I GGWCC 4 cut(s) 249, 403, 484, 615
BmgT120I GGNCC 5 cut(s) 182, 249, 403, 484, 615
BmiI GGNNCC 3 cut(s) 214, 733, 739
BmrFI CCNGG 4 cut(s) 389, 401, 518, 678
BmsI GCATC 2 cut(s) 21, 282
BpiI GAAGAC 1 cut(s) 316
BplI GAGNNNNNCTC 4 cut(s) 344, 376, 512, 544
BpmI CTGGAG 1 cut(s) 740
BpuMI CCSGG 3 cut(s) 389, 518, 678
BsaJI CCNNGG 2 cut(s) 178, 642
BsaWI WCCGGW 1 cut(s) 486
BsaXI ACNNNNNCTCC 2 cut(s) 434, 464
Bsc4I CCNNNNNNNGG 1 cut(s) 493
Bse118I RCCGGY 4 cut(s) 617, 728, 734, 740
Bse3DI GCAATG 1 cut(s) 500
BseBI CCWGG 1 cut(s) 401
BseDI CCNNGG 2 cut(s) 178, 642
BseGI GGATG 2 cut(s) 571, 798
BseLI CCNNNNNNNGG 1 cut(s) 493
BseMI GCAATG 1 cut(s) 500
BseRI GAGGAG 3 cut(s) 29, 424, 737
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 2 cut(s) 257, 426
Bsh1236I CGCG 4 cut(s) 45, 180, 219, 695
BshFI GGCC 5 cut(s) 5, 183, 399, 653, 728
BshNI GGYRCC 3 cut(s) 212, 731, 737
BslI CCNNNNNNNGG 1 cut(s) 493
BsnI GGCC 5 cut(s) 5, 183, 399, 653, 728
Bsp1286I GDGCHC 1 cut(s) 217
Bsp143I GATC 3 cut(s) 57, 497, 813
BspANI GGCC 5 cut(s) 5, 183, 399, 653, 728
BspFNI CGCG 4 cut(s) 45, 180, 219, 695
BspLI GGNNCC 3 cut(s) 214, 733, 739
BspPI GGATC 2 cut(s) 52, 505
BspT107I GGYRCC 3 cut(s) 212, 731, 737
BsrDI GCAATG 1 cut(s) 500
BsrFI RCCGGY 4 cut(s) 617, 728, 734, 740
BssAI RCCGGY 4 cut(s) 617, 728, 734, 740
BssECI CCNNGG 2 cut(s) 178, 642
BssMI GATC 3 cut(s) 57, 497, 813
Bst2UI CCWGG 1 cut(s) 401
Bst4CI ACNGT 1 cut(s) 87
BstC8I GCNNGC 4 cut(s) 175, 217, 223, 227
BstDSI CCRYGG 1 cut(s) 178
BstF5I GGATG 2 cut(s) 571, 798
BstFNI CGCG 4 cut(s) 45, 180, 219, 695
BstHHI GCGC 2 cut(s) 47, 221
BstKTI GATC 3 cut(s) 60, 500, 816
BstMBI GATC 3 cut(s) 57, 497, 813
BstMWI GCNNNNNNNGC 1 cut(s) 189
BstNI CCWGG 1 cut(s) 401
BstNSI RCATGY 1 cut(s) 676
BstSCI CCNGG 4 cut(s) 387, 399, 516, 676
BstSLI GKGCMC 1 cut(s) 217
BstUI CGCG 4 cut(s) 45, 180, 219, 695
BstV1I GCAGC 2 cut(s) 257, 426
BstV2I GAAGAC 1 cut(s) 316
BsuRI GGCC 5 cut(s) 5, 183, 399, 653, 728
BtgI CCRYGG 1 cut(s) 178
BtgZI GCGATG 1 cut(s) 259
BtsCI GGATG 2 cut(s) 571, 798
Cac8I GCNNGC 4 cut(s) 175, 217, 223, 227
CfoI GCGC 2 cut(s) 47, 221
Cfr10I RCCGGY 4 cut(s) 617, 728, 734, 740
Cfr13I GGNCC 5 cut(s) 182, 249, 403, 484, 615
Cfr42I CCGCGG 1 cut(s) 181
CseI GACGC 2 cut(s) 353, 406
Csp6I GTAC 3 cut(s) 358, 490, 670
CviAII CATG 2 cut(s) 659, 673
CviQI GTAC 3 cut(s) 358, 490, 670
DpnI GATC 3 cut(s) 59, 499, 815
DpnII GATC 3 cut(s) 57, 497, 813
DraIII CACNNNGTG 1 cut(s) 532
DrdI GACNNNNNNGTC 1 cut(s) 320
DseDI GACNNNNNNGTC 1 cut(s) 320
EaeI YGGCCR 2 cut(s) 3, 651
EciI GGCGGA 1 cut(s) 11
Eco32I GATATC 1 cut(s) 478
Eco47I GGWCC 4 cut(s) 249, 403, 484, 615
Eco57I CTGAAG 1 cut(s) 250
EcoRII CCWGG 1 cut(s) 399
EcoRV GATATC 1 cut(s) 478
FaeI CATG 2 cut(s) 662, 676
FaiI YATR 4 cut(s) 599, 660, 674, 786
FatI CATG 2 cut(s) 658, 672
FauI CCCGC 5 cut(s) 182, 197, 224, 230, 245
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 8 cut(s) 178, 181, 271, 397, 415, 636, 690, 693
FokI GGATG 2 cut(s) 578, 805
Fsp4HI GCNGC 8 cut(s) 178, 181, 271, 397, 415, 636, 690, 693
GlaI GCGC 2 cut(s) 46, 220
GluI GCNGC 8 cut(s) 178, 181, 271, 397, 415, 636, 690, 693
GsuI CTGGAG 1 cut(s) 740
HaeIII GGCC 5 cut(s) 5, 183, 399, 653, 728
HgaI GACGC 2 cut(s) 353, 406
HhaI GCGC 2 cut(s) 47, 221
Hin1II CATG 2 cut(s) 662, 676
Hin6I GCGC 2 cut(s) 45, 219
HinP1I GCGC 2 cut(s) 45, 219
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HinfI GANTC 4 cut(s) 102, 243, 282, 800
HphI GGTGA 5 cut(s) 88, 142, 451, 692, 755
Hpy166II GTNNAC 1 cut(s) 205
Hpy188I TCNGA 3 cut(s) 261, 576, 604
Hpy188III TCNNGA 2 cut(s) 247, 352
Hpy8I GTNNAC 1 cut(s) 205
Hpy99I CGWCG 5 cut(s) 206, 347, 422, 458, 592
HpyAV CCTTC 5 cut(s) 157, 274, 404, 571, 601
HpyCH4III ACNGT 1 cut(s) 87
HpyCH4IV ACGT 1 cut(s) 330
HpyCH4V TGCA 1 cut(s) 273
HpyF10VI GCNNNNNNNGC 1 cut(s) 189
HpySE526I ACGT 1 cut(s) 330
Hsp92II CATG 2 cut(s) 662, 676
HspAI GCGC 2 cut(s) 45, 219
KspI CCGCGG 1 cut(s) 181
Kzo9I GATC 3 cut(s) 57, 497, 813
LmnI GCTCC 1 cut(s) 234
Lsp1109I GCAGC 2 cut(s) 257, 426
LweI GCATC 2 cut(s) 21, 282
MaeII ACGT 1 cut(s) 330
MaeIII GTNAC 4 cut(s) 331, 457, 524, 767
MalI GATC 3 cut(s) 59, 499, 815
MboI GATC 3 cut(s) 57, 497, 813
MboII GAAGA 7 cut(s) 85, 134, 154, 316, 715, 724, 758
MhlI GDGCHC 1 cut(s) 217
MlsI TGGCCA 2 cut(s) 5, 653
MluCI AATT 1 cut(s) 570
MluNI TGGCCA 2 cut(s) 5, 653
MlyI GAGTC 2 cut(s) 96, 237
MmeI TCCRAC 2 cut(s) 132, 770
Mox20I TGGCCA 2 cut(s) 5, 653
MroXI GAANNNNTTC 1 cut(s) 557
MscI TGGCCA 2 cut(s) 5, 653
Msp20I TGGCCA 2 cut(s) 5, 653
MspA1I CMGCKG 2 cut(s) 180, 270
MspR9I CCNGG 4 cut(s) 389, 401, 518, 678
MvaI CCWGG 1 cut(s) 401
MvnI CGCG 4 cut(s) 45, 180, 219, 695
MwoI GCNNNNNNNGC 1 cut(s) 189
NciI CCSGG 3 cut(s) 389, 518, 678
NdeII GATC 3 cut(s) 57, 497, 813
NlaIII CATG 2 cut(s) 662, 676
NlaIV GGNNCC 3 cut(s) 214, 733, 739
NmuCI GTSAC 3 cut(s) 331, 457, 524
NspI RCATGY 1 cut(s) 676
PcsI WCGNNNNNNNCGW 5 cut(s) 327, 414, 459, 608, 639
PdmI GAANNNNTTC 1 cut(s) 557
PfeI GAWTC 2 cut(s) 282, 800
PfoI TCCNGGA 1 cut(s) 516
PkrI GCNGC 8 cut(s) 179, 182, 272, 398, 416, 637, 691, 694
PleI GAGTC 2 cut(s) 96, 237
PpsI GAGTC 2 cut(s) 96, 237
Psp6I CCWGG 1 cut(s) 399
PspGI CCWGG 1 cut(s) 399
PspN4I GGNNCC 3 cut(s) 214, 733, 739
PspPI GGNCC 5 cut(s) 182, 249, 403, 484, 615
PvuII CAGCTG 1 cut(s) 270
RsaI GTAC 3 cut(s) 359, 491, 671
RsaNI GTAC 3 cut(s) 358, 490, 670
SacII CCGCGG 1 cut(s) 181
SalI GTCGAC 1 cut(s) 203
SatI GCNGC 8 cut(s) 178, 181, 271, 397, 415, 636, 690, 693
Sau3AI GATC 3 cut(s) 57, 497, 813
Sau96I GGNCC 5 cut(s) 182, 249, 403, 484, 615
ScaI AGTACT 1 cut(s) 359
SchI GAGTC 2 cut(s) 96, 237
ScrFI CCNGG 4 cut(s) 389, 401, 518, 678
SduI GDGCHC 1 cut(s) 217
SfaNI GCATC 2 cut(s) 21, 282
Sfr303I CCGCGG 1 cut(s) 181
SgrBI CCGCGG 1 cut(s) 181
SinI GGWCC 4 cut(s) 249, 403, 484, 615
Sse9I AATT 1 cut(s) 570
StyD4I CCNGG 4 cut(s) 387, 399, 516, 676
TaaI ACNGT 1 cut(s) 87
TaiI ACGT 1 cut(s) 333
TaqI TCGA 4 cut(s) 204, 351, 408, 587
TaqII GACCGA 1 cut(s) 488
TasI AATT 1 cut(s) 570
TatI WGTACW 2 cut(s) 357, 669
TauI GCSGC 6 cut(s) 180, 183, 399, 638, 692, 695
TfiI GAWTC 2 cut(s) 282, 800
TseFI GTSAC 3 cut(s) 331, 457, 524
TseI GCWGC 2 cut(s) 270, 414
Tsp45I GTSAC 3 cut(s) 331, 457, 524
TspDTI ATGAA 4 cut(s) 274, 582, 773, 774
TspGWI ACGGA 2 cut(s) 146, 578
VpaK11BI GGWCC 4 cut(s) 249, 403, 484, 615
XapI RAATTY 1 cut(s) 570
XceI RCATGY 1 cut(s) 676
XcmI CCANNNNNNNNNTGG 1 cut(s) 646
XmiI GTMKAC 1 cut(s) 204
XmnI GAANNNNTTC 1 cut(s) 557
ZrmI AGTACT 1 cut(s) 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.