MD03G1034500.v1.1

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0018500
Physical Location & Seq
Reverse (-)
2771175 .. 2775590
4416 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1034500.v1.1.491

Sequence Viewer

Length: 867 bp
ATGCTAGAAAAACAAAACATGGGGTTTGGGTCTCAAATACAAGACATTGTTTCTAAGATTCACCCAGACTGTCAAATATTATTGTTGGGTACTAGCTGGTCAAAGGAGGTTGATAAACGGGCAAACCGGTTCCTTTACAACGCATGCAATGTTACAATTGTTTCTCCACCTCTGAAACCTACACATCCGATCGACCAAACTGTTTACGTCGTTACTGAAGATCAGAAATATAACAAATTGGTAGAGTTGCTAGGTCGTATAGTGGATGGCAGCAGAATACTGATATTCATGGATATAAAGGAAGGATGCGAAAAACGTGATCAGTTTGCCCTGCAGCTTTATAGGGACGGTCAGCATGCAGTCTCGCTTCATGAAAACAAGGGTCAAGCGGAAATGGCCCGGATTGTCTCTGAGTTTCAAGCTGGCAGGATCCCCATAATGATTGCAACAGATGTTGCTGCTCGTGATCTAGATCTGAAGGATGTGAAATATGTTATCAATTACGACTTTCCAGTGTCCCTTGATGATTATGTTTACCGCATTGACCGTGCTGGAAGTGCCGCAGCAAAAGGAACAGTATTCACGTTCTTTACAACTGCTAATGCTGTATTCGCAGAGGAACTTATTATCTTACTCAAGGAAGCTGGACAGAAGGTTAGTTCTGAATTGGAACAACTGGGGCGTGATGTACCATATCCAGGAACCTACATACCTGCACCGTGGCGGTGGGCGGTTAACCATTACATTGGAGCTGGTCATTTCTTTCCGTTGTATCTGCATTGTTCTAAGACCAAGGATATTGCCAGTAACGTCTGTTGTGCGGAAGCGTCAAATATGAAACCAATAAGCAACAACGGCAAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko05202 ko05205 map03040 map05202 map05205
Pfam Domains
Protein Families
Helicase_C

Protein Analysis

289

Amino Acids

32.34

Weight (kDa)

6.39

Isoelectric Point (pI)

25.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Helicase_C PF00271 76 - 186 4.3e-20 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 721
AciI CCGC 6 cut(s) 389, 538, 561, 724, 731, 821
AclWI GGATC 2 cut(s) 424, 437
AcuI CTGAAG 2 cut(s) 237, 497
AfaI GTAC 2 cut(s) 91, 690
AfiI CCNNNNNNNGG 1 cut(s) 698
AgeI ACCGGT 1 cut(s) 126
AgsI TTSAA 1 cut(s) 419
AjnI CCWGG 1 cut(s) 697
AluBI AGCT 5 cut(s) 96, 337, 422, 644, 752
AluI AGCT 5 cut(s) 96, 337, 422, 644, 752
Alw26I GTCTC 3 cut(s) 36, 367, 412
AlwI GGATC 2 cut(s) 424, 437
AoxI GGCC 1 cut(s) 396
ApeKI GCWGC 4 cut(s) 270, 334, 458, 563
AsiGI ACCGGT 1 cut(s) 126
AspS9I GGNCC 1 cut(s) 397
AsuC2I CCSGG 1 cut(s) 400
AsuHPI GGTGA 1 cut(s) 53
BamHI GGATCC 1 cut(s) 429
BauI CACGAG 1 cut(s) 462
BbvI GCAGC 4 cut(s) 282, 346, 445, 575
BccI CCATC 1 cut(s) 260
BciT130I CCWGG 1 cut(s) 699
BclI TGATCA 1 cut(s) 319
BcnI CCSGG 1 cut(s) 400
BcoDI GTCTC 3 cut(s) 36, 367, 412
BfaI CTAG 4 cut(s) 5, 93, 251, 470
BfmI CTRYAG 1 cut(s) 332
BfuAI ACCTGC 1 cut(s) 721
BglII AGATCT 1 cut(s) 472
BisI GCNGC 5 cut(s) 271, 335, 459, 561, 564
BlsI GCNGC 5 cut(s) 272, 336, 460, 562, 565
Bme1390I CCNGG 2 cut(s) 400, 699
BmgT120I GGNCC 1 cut(s) 397
BmiI GGNNCC 3 cut(s) 131, 431, 703
BmrFI CCNGG 2 cut(s) 400, 699
BmrI ACTGGG 1 cut(s) 686
BmsI GCATC 1 cut(s) 296
BmuI ACTGGG 1 cut(s) 686
BpuEI CTTGAG 1 cut(s) 620
BpuMI CCSGG 1 cut(s) 400
BsaBI GATNNNNATC 1 cut(s) 471
BsaI GGTCTC 1 cut(s) 36
BsaJI CCNNGG 2 cut(s) 719, 792
BsaWI WCCGGW 1 cut(s) 126
Bsc4I CCNNNNNNNGG 1 cut(s) 698
Bse118I RCCGGY 1 cut(s) 126
Bse1I ACTGG 3 cut(s) 512, 681, 804
Bse3DI GCAATG 1 cut(s) 154
Bse8I GATNNNNATC 1 cut(s) 471
BseBI CCWGG 1 cut(s) 699
BseDI CCNNGG 2 cut(s) 719, 792
BseGI GGATG 4 cut(s) 184, 271, 311, 487
BseJI GATNNNNATC 1 cut(s) 471
BseLI CCNNNNNNNGG 1 cut(s) 698
BseMI GCAATG 1 cut(s) 154
BseMII CTCAG 1 cut(s) 402
BseNI ACTGG 3 cut(s) 512, 681, 804
BseXI GCAGC 4 cut(s) 282, 346, 445, 575
BsgI GTGCAG 1 cut(s) 699
Bsh1285I CGRYCG 1 cut(s) 192
BshFI GGCC 1 cut(s) 398
BshTI ACCGGT 1 cut(s) 126
BsiEI CGRYCG 1 cut(s) 192
BsiSI CCGG 2 cut(s) 127, 400
BslFI GGGAC 2 cut(s) 359, 502
BslI CCNNNNNNNGG 1 cut(s) 698
BsmAI GTCTC 3 cut(s) 36, 367, 412
BsmFI GGGAC 2 cut(s) 359, 502
BsnI GGCC 1 cut(s) 398
Bso31I GGTCTC 1 cut(s) 36
Bsp143I GATC 6 cut(s) 189, 220, 319, 429, 466, 472
BspACI CCGC 6 cut(s) 389, 538, 561, 724, 731, 821
BspANI GGCC 1 cut(s) 398
BspCNI CTCAG 1 cut(s) 403
BspHI TCATGA 1 cut(s) 370
BspLI GGNNCC 3 cut(s) 131, 431, 703
BspMAI CTGCAG 1 cut(s) 336
BspMI ACCTGC 1 cut(s) 721
BspPI GGATC 2 cut(s) 424, 437
BspTNI GGTCTC 1 cut(s) 36
BsrDI GCAATG 1 cut(s) 154
BsrFI RCCGGY 1 cut(s) 126
BsrI ACTGG 3 cut(s) 512, 681, 804
BssAI RCCGGY 1 cut(s) 126
BssECI CCNNGG 2 cut(s) 719, 792
BssMI GATC 6 cut(s) 189, 220, 319, 429, 466, 472
BssSI CACGAG 1 cut(s) 462
BssT1I CCWWGG 1 cut(s) 792
Bst2BI CACGAG 1 cut(s) 462
Bst2UI CCWGG 1 cut(s) 699
Bst4CI ACNGT 6 cut(s) 71, 202, 350, 548, 577, 720
BstC8I GCNNGC 3 cut(s) 145, 357, 424
BstDEI CTNAG 3 cut(s) 54, 411, 786
BstDSI CCRYGG 1 cut(s) 719
BstF5I GGATG 4 cut(s) 184, 271, 311, 487
BstKTI GATC 6 cut(s) 192, 223, 322, 432, 469, 475
BstMAI GTCTC 3 cut(s) 36, 367, 412
BstMBI GATC 6 cut(s) 189, 220, 319, 429, 466, 472
BstMCI CGRYCG 1 cut(s) 192
BstMWI GCNNNNNNNGC 4 cut(s) 395, 557, 611, 855
BstNI CCWGG 1 cut(s) 699
BstNSI RCATGY 2 cut(s) 147, 359
BstSCI CCNGG 2 cut(s) 398, 697
BstSFI CTRYAG 1 cut(s) 332
BstV1I GCAGC 4 cut(s) 282, 346, 445, 575
BstX2I RGATCY 2 cut(s) 429, 472
BstXI CCANNNNNNTGG 1 cut(s) 746
BstYI RGATCY 2 cut(s) 429, 472
BsuRI GGCC 1 cut(s) 398
BtgI CCRYGG 1 cut(s) 719
BtsCI GGATG 4 cut(s) 184, 271, 311, 487
BtsIMutI CAGTG 1 cut(s) 519
BveI ACCTGC 1 cut(s) 721
Cac8I GCNNGC 3 cut(s) 145, 357, 424
CciI TCATGA 1 cut(s) 370
Cfr10I RCCGGY 1 cut(s) 126
Cfr13I GGNCC 1 cut(s) 397
CseI GACGC 1 cut(s) 816
Csp6I GTAC 2 cut(s) 90, 689
CspAI ACCGGT 1 cut(s) 126
CviAII CATG 5 cut(s) 19, 144, 289, 356, 371
CviJI RGCY 6 cut(s) 96, 337, 398, 422, 644, 752
CviKI_1 RGCY 6 cut(s) 96, 337, 398, 422, 644, 752
CviQI GTAC 2 cut(s) 90, 689
DdeI CTNAG 3 cut(s) 54, 411, 786
DpnI GATC 6 cut(s) 191, 222, 321, 431, 468, 474
DpnII GATC 6 cut(s) 189, 220, 319, 429, 466, 472
Eco130I CCWWGG 1 cut(s) 792
Eco31I GGTCTC 1 cut(s) 36
Eco57I CTGAAG 2 cut(s) 237, 497
EcoRII CCWGG 1 cut(s) 697
EcoT14I CCWWGG 1 cut(s) 792
ErhI CCWWGG 1 cut(s) 792
FaeI CATG 5 cut(s) 22, 147, 292, 359, 374
FaqI GGGAC 2 cut(s) 359, 502
FatI CATG 5 cut(s) 18, 143, 288, 355, 370
FbaI TGATCA 1 cut(s) 319
Fnu4HI GCNGC 5 cut(s) 271, 335, 459, 561, 564
FokI GGATG 4 cut(s) 171, 278, 318, 494
Fsp4HI GCNGC 5 cut(s) 271, 335, 459, 561, 564
FspBI CTAG 4 cut(s) 5, 93, 251, 470
GluI GCNGC 5 cut(s) 271, 335, 459, 561, 564
HaeIII GGCC 1 cut(s) 398
HapII CCGG 2 cut(s) 127, 400
HgaI GACGC 1 cut(s) 816
Hin1II CATG 5 cut(s) 22, 147, 292, 359, 374
HincII GTYRAC 1 cut(s) 736
HindII GTYRAC 1 cut(s) 736
HinfI GANTC 1 cut(s) 58
HpaI GTTAAC 1 cut(s) 736
HpaII CCGG 2 cut(s) 127, 400
HphI GGTGA 1 cut(s) 53
Hpy166II GTNNAC 3 cut(s) 205, 535, 736
Hpy188I TCNGA 6 cut(s) 174, 189, 225, 412, 477, 664
Hpy188III TCNNGA 3 cut(s) 371, 464, 470
Hpy8I GTNNAC 3 cut(s) 205, 535, 736
Hpy99I CGWCG 1 cut(s) 212
HpyAV CCTTC 3 cut(s) 296, 472, 646
HpyCH4III ACNGT 6 cut(s) 71, 202, 350, 548, 577, 720
HpyCH4IV ACGT 4 cut(s) 207, 316, 584, 810
HpyCH4V TGCA 6 cut(s) 147, 334, 359, 446, 716, 778
HpyF10VI GCNNNNNNNGC 4 cut(s) 395, 557, 611, 855
HpyF3I CTNAG 3 cut(s) 54, 411, 786
HpySE526I ACGT 4 cut(s) 207, 316, 584, 810
Hsp92II CATG 5 cut(s) 22, 147, 292, 359, 374
Ksp22I TGATCA 1 cut(s) 319
KspAI GTTAAC 1 cut(s) 736
Kzo9I GATC 6 cut(s) 189, 220, 319, 429, 466, 472
LmnI GCTCC 1 cut(s) 749
Lsp1109I GCAGC 4 cut(s) 282, 346, 445, 575
LweI GCATC 1 cut(s) 296
MaeI CTAG 4 cut(s) 5, 93, 251, 470
MaeII ACGT 4 cut(s) 207, 316, 584, 810
MaeIII GTNAC 3 cut(s) 151, 211, 806
MalI GATC 6 cut(s) 191, 222, 321, 431, 468, 474
MboI GATC 6 cut(s) 189, 220, 319, 429, 466, 472
MboII GAAGA 1 cut(s) 230
MfeI CAATTG 1 cut(s) 156
MflI RGATCY 2 cut(s) 429, 472
MluCI AATT 4 cut(s) 156, 236, 499, 665
MnlI CCTC 3 cut(s) 100, 180, 610
MseI TTAA 1 cut(s) 735
MspI CCGG 2 cut(s) 127, 400
MspR9I CCNGG 2 cut(s) 400, 699
MunI CAATTG 1 cut(s) 156
MvaI CCWGG 1 cut(s) 699
MwoI GCNNNNNNNGC 4 cut(s) 395, 557, 611, 855
NciI CCSGG 1 cut(s) 400
NdeII GATC 6 cut(s) 189, 220, 319, 429, 466, 472
NlaIII CATG 5 cut(s) 22, 147, 292, 359, 374
NlaIV GGNNCC 3 cut(s) 131, 431, 703
NspI RCATGY 2 cut(s) 147, 359
PaeI GCATGC 2 cut(s) 147, 359
PagI TCATGA 1 cut(s) 370
PfeI GAWTC 1 cut(s) 58
PfoI TCCNGGA 1 cut(s) 697
PinAI ACCGGT 1 cut(s) 126
PkrI GCNGC 5 cut(s) 272, 336, 460, 562, 565
Ple19I CGATCG 1 cut(s) 192
Psp6I CCWGG 1 cut(s) 697
PspGI CCWGG 1 cut(s) 697
PspN4I GGNNCC 3 cut(s) 131, 431, 703
PspPI GGNCC 1 cut(s) 397
PstI CTGCAG 1 cut(s) 336
PsuI RGATCY 2 cut(s) 429, 472
PvuI CGATCG 1 cut(s) 192
RsaI GTAC 2 cut(s) 91, 690
RsaNI GTAC 2 cut(s) 90, 689
SaqAI TTAA 1 cut(s) 735
SatI GCNGC 5 cut(s) 271, 335, 459, 561, 564
Sau3AI GATC 6 cut(s) 189, 220, 319, 429, 466, 472
Sau96I GGNCC 1 cut(s) 397
ScrFI CCNGG 2 cut(s) 400, 699
SfaNI GCATC 1 cut(s) 296
SfcI CTRYAG 1 cut(s) 332
SmlI CTYRAG 1 cut(s) 635
SmoI CTYRAG 1 cut(s) 635
SphI GCATGC 2 cut(s) 147, 359
Sse9I AATT 4 cut(s) 156, 236, 499, 665
SsiI CCGC 6 cut(s) 389, 538, 561, 724, 731, 821
SspI AATATT 1 cut(s) 78
SspMI CTAG 4 cut(s) 5, 93, 251, 470
StyD4I CCNGG 2 cut(s) 398, 697
StyI CCWWGG 1 cut(s) 792
TaaI ACNGT 6 cut(s) 71, 202, 350, 548, 577, 720
TaiI ACGT 4 cut(s) 210, 319, 587, 813
TaqI TCGA 1 cut(s) 192
TasI AATT 4 cut(s) 156, 236, 499, 665
TauI GCSGC 1 cut(s) 563
TfiI GAWTC 1 cut(s) 58
Tru1I TTAA 1 cut(s) 735
Tru9I TTAA 1 cut(s) 735
TscAI CASTG 1 cut(s) 519
TseI GCWGC 4 cut(s) 270, 334, 458, 563
TspDTI ATGAA 4 cut(s) 277, 359, 387, 851
TspGWI ACGGA 1 cut(s) 756
TspRI CASTG 1 cut(s) 519
XbaI TCTAGA 1 cut(s) 469
XceI RCATGY 2 cut(s) 147, 359
XspI CTAG 4 cut(s) 5, 93, 251, 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.