MD17G1170000.v1.1

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0018500
Physical Location & Seq
Reverse (-)
17333086 .. 17335672
2587 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1170000.v1.1.491

Sequence Viewer

Length: 1272 bp
ATGGCGAAGCGCTCGACTGACCCCAATTCAGTAACCCTAGCACAACATTATGGTGCTGATGCTGCTCAAGGCTCCTCAAATACGGCCGGTGAGGGCAAGGAGTATAGGAGCAAGCGGAGGAAAATTACTGCTCAAGGCGGCACGTCTGAAACGCAGGACCACCACAAGAGGGAAATTACTACTGATGGCACCTCTTATACGCCCGACAACGTCGAGGAGTATAGGCTACAGAGGGGAATTCGAGTTGTAGGCTGGGATGATGTTCCACACCCCATTAGGACTTTCGATGACACTCAGTTTCCGGAAAATGTTATACAAGAATTTTCAAAAGCTGGATTTGTCGGACCTACACCAATTCAAGCTCAAGTATGGCCAATGGATTTGGAGGACTGTGACCTCATTGGTATTGCCCAACCAGGATCCGGGAAGATGCTAGCGTATCTGATCCCTGCAATTCTCCATGCAAACTCCCAGCCAAAACGATCTCGTGGTGGAGATGGCCCAATAGTATTGATTTTAGCTCCAAACACTCAACGCGCTGAACAAATCCATCAAGAGTGTGCTAAATTTGCTGCTGCTTACAACTTTAAAGTCTTATTCGGCTTGTCCCTGCTTAAGCCCGAAAATTGTGGTCAGACTTGTGGAGGTGGGATTTTAATCTACACACTTGGTGGCTTGCTTCTTTCGTTAAAGAGAAAACGTACAAACTTGAAAAGCGTCACATACCTTGTGTTGGATGGTGCTGATTGGATGCTATCAGACACGCATGAGCCTCAAATAAGAAATCTATTATCTCAGATTCGCTTAGACTGTCAAAAATCATTCTGGGGTAGTAGCTGGCCAGAAGATGTTGATAAACGGGCAAAACTATTCCTTCACAACCCATGCAAAGTTCAAATAATTTCTCCAGATCTGAAAGTGACGCCTCCGGTGGACCAAATTGTTTACGTCCTTACTGAGGATCAGAAATATAACAAGTTGGTTGCGCTACTAGATGGTATATTGGATGGCAGCAGAGTAGTGATATTCATGGATACGAAGAAAGGATGTGAATCACAAGATCAGATCGCTCTGCAGCTTTGCACGGACGGTCTGCACGCAGTCTCGTTTCATGAAAACAAAAGTCAAGCGGAAAGGGTCGGTATTGCCTCTGAGTTTCAAGCTGGTAAGATCCCCATAATGATTGCAACAGATGTAGCCGCTCATGTTCTAGATCTGAAGGATGTGAAATATGTTATCAATTACGACTTTCCAGGATCCCTTGATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko05202 ko05205 map03040 map05202 map05205
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

424

Amino Acids

46.76

Weight (kDa)

6.02

Isoelectric Point (pI)

35.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 117 - 271 2.6e-21 DEAD/DEAH box helicase
Helicase_C PF00271 323 - 421 1.7e-16 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 188
AccBSI CCGCTC 1 cut(s) 1202
AccII CGCG 1 cut(s) 537
AccIII TCCGGA 1 cut(s) 301
AciI CCGC 4 cut(s) 115, 138, 1130, 1200
AclWI GGATC 7 cut(s) 414, 427, 439, 969, 1165, 1251, 1264
AcoI YGGCCR 3 cut(s) 84, 371, 839
AcsI RAATTY 3 cut(s) 237, 320, 566
AcuI CTGAAG 1 cut(s) 1238
AcyI GRCGYC 1 cut(s) 923
AdeI CACNNNGTG 1 cut(s) 671
AfaI GTAC 1 cut(s) 703
AfeI AGCGCT 1 cut(s) 11
AfiI CCNNNNNNNGG 4 cut(s) 169, 422, 733, 958
AflII CTTAAG 1 cut(s) 614
AgsI TTSAA 5 cut(s) 327, 359, 712, 896, 1160
AjiI CACGTC 1 cut(s) 144
AjnI CCWGG 2 cut(s) 415, 1252
AluBI AGCT 6 cut(s) 332, 362, 521, 837, 1078, 1163
AluI AGCT 6 cut(s) 332, 362, 521, 837, 1078, 1163
Alw26I GTCTC 1 cut(s) 1108
AlwI GGATC 7 cut(s) 414, 427, 439, 969, 1165, 1251, 1264
Aor13HI TCCGGA 1 cut(s) 301
Aor51HI AGCGCT 1 cut(s) 11
AoxI GGCC 4 cut(s) 84, 371, 499, 839
ApeKI GCWGC 5 cut(s) 62, 572, 575, 1011, 1075
ApoI RAATTY 3 cut(s) 237, 320, 566
AspLEI GCGC 3 cut(s) 12, 539, 988
AspS9I GGNCC 4 cut(s) 157, 344, 500, 934
AsuC2I CCSGG 1 cut(s) 424
AsuHPI GGTGA 1 cut(s) 101
AsuNHI GCTAGC 1 cut(s) 433
AvaII GGWCC 3 cut(s) 157, 344, 934
BalI TGGCCA 2 cut(s) 373, 841
BamHI GGATCC 2 cut(s) 419, 1256
BanI GGYRCC 1 cut(s) 188
BauI CACGAG 1 cut(s) 486
BbvI GCAGC 5 cut(s) 49, 559, 562, 1023, 1087
BccI CCATC 6 cut(s) 179, 491, 558, 731, 989, 1001
BceAI ACGGC 1 cut(s) 99
BciT130I CCWGG 2 cut(s) 417, 1254
BciVI GTATCC 1 cut(s) 1027
BcnI CCSGG 1 cut(s) 424
BcoDI GTCTC 1 cut(s) 1108
BfaI CTAG 4 cut(s) 38, 434, 992, 1211
BfmI CTRYAG 2 cut(s) 227, 1073
BfoI RGCGCY 1 cut(s) 13
BfrI CTTAAG 1 cut(s) 614
BfuI GTATCC 1 cut(s) 1027
BglII AGATCT 2 cut(s) 910, 1213
BisI GCNGC 7 cut(s) 63, 139, 573, 576, 1012, 1076, 1200
BlsI GCNGC 7 cut(s) 64, 140, 574, 577, 1013, 1077, 1201
Bme1390I CCNGG 3 cut(s) 417, 424, 1254
Bme18I GGWCC 3 cut(s) 157, 344, 934
BmgBI CACGTC 1 cut(s) 144
BmgT120I GGNCC 4 cut(s) 157, 344, 500, 934
BmiI GGNNCC 4 cut(s) 73, 190, 421, 1258
BmrFI CCNGG 3 cut(s) 417, 424, 1254
BmsI GCATC 3 cut(s) 49, 420, 741
BmtI GCTAGC 1 cut(s) 437
BpmI CTGGAG 1 cut(s) 891
BpuEI CTTGAG 3 cut(s) 51, 117, 348
BpuMI CCSGG 1 cut(s) 424
BsaBI GATNNNNATC 2 cut(s) 656, 1051
BsaHI GRCGYC 1 cut(s) 923
BsaWI WCCGGW 2 cut(s) 301, 928
Bsc4I CCNNNNNNNGG 4 cut(s) 169, 422, 733, 958
Bse118I RCCGGY 1 cut(s) 86
Bse8I GATNNNNATC 2 cut(s) 656, 1051
BseAI TCCGGA 1 cut(s) 301
BseBI CCWGG 2 cut(s) 417, 1254
BseGI GGATG 6 cut(s) 262, 742, 756, 1012, 1052, 1228
BseJI GATNNNNATC 2 cut(s) 656, 1051
BseLI CCNNNNNNNGG 4 cut(s) 169, 422, 733, 958
BseMII CTCAG 4 cut(s) 308, 809, 948, 1143
BseRI GAGGAG 2 cut(s) 64, 230
BseX3I CGGCCG 1 cut(s) 84
BseXI GCAGC 5 cut(s) 49, 559, 562, 1023, 1087
BseYI CCCAGC 2 cut(s) 252, 471
BsgI GTGCAG 1 cut(s) 1079
Bsh1236I CGCG 1 cut(s) 537
Bsh1285I CGRYCG 1 cut(s) 87
BshFI GGCC 4 cut(s) 86, 373, 501, 841
BshNI GGYRCC 1 cut(s) 188
BsiEI CGRYCG 1 cut(s) 87
BsiSI CCGG 4 cut(s) 87, 302, 423, 929
BslFI GGGAC 1 cut(s) 592
BslI CCNNNNNNNGG 4 cut(s) 169, 422, 733, 958
BsmAI GTCTC 1 cut(s) 1108
BsmFI GGGAC 1 cut(s) 592
BsnI GGCC 4 cut(s) 86, 373, 501, 841
Bsp13I TCCGGA 1 cut(s) 301
BspACI CCGC 4 cut(s) 115, 138, 1130, 1200
BspANI GGCC 4 cut(s) 86, 373, 501, 841
BspCNI CTCAG 4 cut(s) 307, 808, 949, 1144
BspEI TCCGGA 1 cut(s) 301
BspFNI CGCG 1 cut(s) 537
BspHI TCATGA 1 cut(s) 1111
BspLI GGNNCC 4 cut(s) 73, 190, 421, 1258
BspMAI CTGCAG 1 cut(s) 1077
BspOI GCTAGC 1 cut(s) 437
BspPI GGATC 7 cut(s) 414, 427, 439, 969, 1165, 1251, 1264
BspT107I GGYRCC 1 cut(s) 188
BspTI CTTAAG 1 cut(s) 614
BsrBI CCGCTC 1 cut(s) 1202
BsrFI RCCGGY 1 cut(s) 86
BssAI RCCGGY 1 cut(s) 86
BssNI GRCGYC 1 cut(s) 923
BssSI CACGAG 1 cut(s) 486
Bst2BI CACGAG 1 cut(s) 486
Bst2UI CCWGG 2 cut(s) 417, 1254
Bst4CI ACNGT 3 cut(s) 392, 812, 1091
BstACI GRCGYC 1 cut(s) 923
BstAFI CTTAAG 1 cut(s) 614
BstC8I GCNNGC 5 cut(s) 113, 435, 677, 839, 1098
BstDEI CTNAG 5 cut(s) 294, 795, 805, 957, 1152
BstENI CCTNNNNNAGG 1 cut(s) 956
BstF5I GGATG 6 cut(s) 262, 742, 756, 1012, 1052, 1228
BstFNI CGCG 1 cut(s) 537
BstH2I RGCGCY 1 cut(s) 13
BstHHI GCGC 3 cut(s) 12, 539, 988
BstMAI GTCTC 1 cut(s) 1108
BstMCI CGRYCG 1 cut(s) 87
BstMWI GCNNNNNNNGC 2 cut(s) 62, 569
BstNI CCWGG 2 cut(s) 417, 1254
BstSCI CCNGG 3 cut(s) 415, 422, 1252
BstSFI CTRYAG 2 cut(s) 227, 1073
BstUI CGCG 1 cut(s) 537
BstV1I GCAGC 5 cut(s) 49, 559, 562, 1023, 1087
BstX2I RGATCY 5 cut(s) 419, 910, 1170, 1213, 1256
BstYI RGATCY 5 cut(s) 419, 910, 1170, 1213, 1256
BstZI CGGCCG 1 cut(s) 84
BsuI GTATCC 1 cut(s) 1027
BsuRI GGCC 4 cut(s) 86, 373, 501, 841
BtrI CACGTC 1 cut(s) 144
BtsCI GGATG 6 cut(s) 262, 742, 756, 1012, 1052, 1228
Cac8I GCNNGC 5 cut(s) 113, 435, 677, 839, 1098
CciI TCATGA 1 cut(s) 1111
CfoI GCGC 3 cut(s) 12, 539, 988
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 4 cut(s) 157, 344, 500, 934
CseI GACGC 2 cut(s) 706, 931
Csp6I GTAC 1 cut(s) 702
CviAII CATG 6 cut(s) 461, 767, 885, 1030, 1112, 1205
CviQI GTAC 1 cut(s) 702
DdeI CTNAG 5 cut(s) 294, 795, 805, 957, 1152
DraI TTTAAA 1 cut(s) 589
DraIII CACNNNGTG 1 cut(s) 671
EaeI YGGCCR 3 cut(s) 84, 371, 839
EagI CGGCCG 1 cut(s) 84
EclXI CGGCCG 1 cut(s) 84
Eco47I GGWCC 3 cut(s) 157, 344, 934
Eco47III AGCGCT 1 cut(s) 11
Eco52I CGGCCG 1 cut(s) 84
Eco57I CTGAAG 1 cut(s) 1238
EcoNI CCTNNNNNAGG 1 cut(s) 956
EcoRI GAATTC 1 cut(s) 237
EcoRII CCWGG 2 cut(s) 415, 1252
FaeI CATG 6 cut(s) 464, 770, 888, 1033, 1115, 1208
FaqI GGGAC 1 cut(s) 592
FatI CATG 6 cut(s) 460, 766, 884, 1029, 1111, 1204
Fnu4HI GCNGC 7 cut(s) 63, 139, 573, 576, 1012, 1076, 1200
FokI GGATG 6 cut(s) 269, 749, 763, 1019, 1059, 1235
Fsp4HI GCNGC 7 cut(s) 63, 139, 573, 576, 1012, 1076, 1200
FspBI CTAG 4 cut(s) 38, 434, 992, 1211
GlaI GCGC 3 cut(s) 11, 538, 987
GluI GCNGC 7 cut(s) 63, 139, 573, 576, 1012, 1076, 1200
GsaI CCCAGC 2 cut(s) 256, 475
GsuI CTGGAG 1 cut(s) 891
HaeII RGCGCY 1 cut(s) 13
HaeIII GGCC 4 cut(s) 86, 373, 501, 841
HapII CCGG 4 cut(s) 87, 302, 423, 929
HgaI GACGC 2 cut(s) 706, 931
HhaI GCGC 3 cut(s) 12, 539, 988
Hin1I GRCGYC 1 cut(s) 923
Hin1II CATG 6 cut(s) 464, 770, 888, 1033, 1115, 1208
Hin6I GCGC 3 cut(s) 10, 537, 986
HinP1I GCGC 3 cut(s) 10, 537, 986
HinfI GANTC 2 cut(s) 799, 1052
HpaII CCGG 4 cut(s) 87, 302, 423, 929
HphI GGTGA 1 cut(s) 101
Hpy166II GTNNAC 2 cut(s) 934, 946
Hpy188III TCNNGA 5 cut(s) 302, 554, 908, 1112, 1211
Hpy8I GTNNAC 2 cut(s) 934, 946
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 2 cut(s) 884, 1213
HpyCH4III ACNGT 3 cut(s) 392, 812, 1091
HpyCH4IV ACGT 4 cut(s) 143, 210, 700, 948
HpyCH4V TGCA 7 cut(s) 452, 464, 888, 1075, 1083, 1096, 1187
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 569
HpyF3I CTNAG 5 cut(s) 294, 795, 805, 957, 1152
HpySE526I ACGT 4 cut(s) 143, 210, 700, 948
Hsp92I GRCGYC 1 cut(s) 923
Hsp92II CATG 6 cut(s) 464, 770, 888, 1033, 1115, 1208
HspAI GCGC 3 cut(s) 10, 537, 986
Kpn2I TCCGGA 1 cut(s) 301
LmnI GCTCC 3 cut(s) 77, 108, 526
Lsp1109I GCAGC 5 cut(s) 49, 559, 562, 1023, 1087
LweI GCATC 3 cut(s) 49, 420, 741
MaeI CTAG 4 cut(s) 38, 434, 992, 1211
MaeII ACGT 4 cut(s) 143, 210, 700, 948
MaeIII GTNAC 4 cut(s) 31, 392, 718, 919
MbiI CCGCTC 1 cut(s) 1202
MboII GAAGA 3 cut(s) 439, 857, 1051
MflI RGATCY 5 cut(s) 419, 910, 1170, 1213, 1256
MlsI TGGCCA 2 cut(s) 373, 841
MluNI TGGCCA 2 cut(s) 373, 841
MmeI TCCRAC 2 cut(s) 322, 714
Mox20I TGGCCA 2 cut(s) 373, 841
MroI TCCGGA 1 cut(s) 301
MscI TGGCCA 2 cut(s) 373, 841
MseI TTAA 5 cut(s) 588, 615, 656, 689, 1270
MslI CAYNNNNRTG 1 cut(s) 51
Msp20I TGGCCA 2 cut(s) 373, 841
MspCI CTTAAG 1 cut(s) 614
MspI CCGG 4 cut(s) 87, 302, 423, 929
MspR9I CCNGG 3 cut(s) 417, 424, 1254
MvaI CCWGG 2 cut(s) 417, 1254
MvnI CGCG 1 cut(s) 537
MwoI GCNNNNNNNGC 2 cut(s) 62, 569
NciI CCSGG 1 cut(s) 424
NheI GCTAGC 1 cut(s) 433
NlaIII CATG 6 cut(s) 464, 770, 888, 1033, 1115, 1208
NlaIV GGNNCC 4 cut(s) 73, 190, 421, 1258
NmuCI GTSAC 3 cut(s) 392, 718, 919
PagI TCATGA 1 cut(s) 1111
PfeI GAWTC 2 cut(s) 799, 1052
PflFI GACNNNGTC 1 cut(s) 209
PfoI TCCNGGA 2 cut(s) 422, 1252
PkrI GCNGC 7 cut(s) 64, 140, 574, 577, 1013, 1077, 1201
Psp6I CCWGG 2 cut(s) 415, 1252
PspFI CCCAGC 2 cut(s) 252, 471
PspGI CCWGG 2 cut(s) 415, 1252
PspN4I GGNNCC 4 cut(s) 73, 190, 421, 1258
PspPI GGNCC 4 cut(s) 157, 344, 500, 934
PstI CTGCAG 1 cut(s) 1077
PsuI RGATCY 5 cut(s) 419, 910, 1170, 1213, 1256
PsyI GACNNNGTC 1 cut(s) 209
RsaI GTAC 1 cut(s) 703
RsaNI GTAC 1 cut(s) 702
RseI CAYNNNNRTG 1 cut(s) 51
SaqAI TTAA 5 cut(s) 588, 615, 656, 689, 1270
SatI GCNGC 7 cut(s) 63, 139, 573, 576, 1012, 1076, 1200
Sau96I GGNCC 4 cut(s) 157, 344, 500, 934
ScrFI CCNGG 3 cut(s) 417, 424, 1254
SfaNI GCATC 3 cut(s) 49, 420, 741
SfcI CTRYAG 2 cut(s) 227, 1073
SinI GGWCC 3 cut(s) 157, 344, 934
SmiMI CAYNNNNRTG 1 cut(s) 51
SmlI CTYRAG 4 cut(s) 66, 132, 363, 614
SmoI CTYRAG 4 cut(s) 66, 132, 363, 614
SsiI CCGC 4 cut(s) 115, 138, 1130, 1200
SspMI CTAG 4 cut(s) 38, 434, 992, 1211
StyD4I CCNGG 3 cut(s) 415, 422, 1252
TaaI ACNGT 3 cut(s) 392, 812, 1091
TaiI ACGT 4 cut(s) 146, 213, 703, 951
TaqI TCGA 4 cut(s) 14, 213, 241, 285
TauI GCSGC 2 cut(s) 141, 1202
TfiI GAWTC 2 cut(s) 799, 1052
Tru1I TTAA 5 cut(s) 588, 615, 656, 689, 1270
Tru9I TTAA 5 cut(s) 588, 615, 656, 689, 1270
TseFI GTSAC 3 cut(s) 392, 718, 919
TseI GCWGC 5 cut(s) 62, 572, 575, 1011, 1075
Tsp45I GTSAC 3 cut(s) 392, 718, 919
TspDTI ATGAA 3 cut(s) 1018, 1100, 1128
TspGWI ACGGA 1 cut(s) 1100
Tth111I GACNNNGTC 1 cut(s) 209
Vha464I CTTAAG 1 cut(s) 614
VpaK11BI GGWCC 3 cut(s) 157, 344, 934
XagI CCTNNNNNAGG 1 cut(s) 956
XapI RAATTY 3 cut(s) 237, 320, 566
XbaI TCTAGA 1 cut(s) 1210
XspI CTAG 4 cut(s) 38, 434, 992, 1211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.