MD17G1170100.v1.1

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0018500
Physical Location & Seq
Reverse (-)
17341826 .. 17344245
2420 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1170100.v1.1.491

Sequence Viewer

Length: 960 bp
ATGACTTTCGATAACGCTCGGTTTCCAGAACATGTTATACAAGAATTTTCAAAAGCTGGATTTGTTCGACCTACACCAATTCAAGCTCAAGTATGGCCAATGGATTTGGAGGACCGTGACCTCATTGGTATTGCTCAACTAGGATCCGGGAAGATGCTAGGGTATCTGATCCCTGCAATTCTCCATGTAAACTCCCAGCCAAAACGATCTCGTGGTGGAGATGGCCCAATAGTATTGATTTTAGCTCCAACAACTCAACGCGCTGAACAAATCCAACAGGAGGGTGCAAAATTTGCTGCAGCTTACAACATTAAAGTCTTATCCGGTTTGTACCTGCTTAAGCATGAAAATTCTGGTCAGACTTGTGGCATTGGTTATGGTAACATGCAATTTTGTATCCGCTTAGACTGTCAAAAATCATTCTGGGGTGCTAGCTGGCCAGAAGAGGTTGATAAACGGGCAAAACTATTCCTTCACAAGCATGCAAAGTTGGTCGAGCTGCTAGGTGGTATATTGGATGGCAGCAGAATAGTTATATTCATGGATACAAAGAAAGGATGTGAATCAGGTGATCAGATCGCCCTGCAGCTTTGCACGGACGGTCAGCACGCAGTCTTGGTTCATGAAAACGAAAGTCAAGAGGAAAGGGTCCGGATTGTCTCTGAGTTTCAAGCTGGCAAGATCCCCATAATGATTGCAACAGATGTTGCCGCTCGTGATCTAGATCTGAAGGATGTGAAATATGTTATCAATTACGACTTTCCGGGATCCCTTGATGATTATGTTTACCACATTGACTGCTTTGGAAGTGCTGCAGCAAAAGGAACAGCATACACATTCTTTACAACTGCTAATGCTGGATGCGCAAAGGGACTTACCATCTTACTCAAGAAAGCTGGACAGAATGTTAGTTCTGAATTGGAACAACTGGGGCGTGATGTAACATATCCAGGTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko05202 ko05205 map03040 map05202 map05205
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

320

Amino Acids

35.11

Weight (kDa)

6.08

Isoelectric Point (pI)

30.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 25 - 108 6e-12 DEAD/DEAH box helicase
Helicase_C PF00271 161 - 262 4.4e-18 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 865
Acc36I ACCTGC 1 cut(s) 342
AccBSI CCGCTC 1 cut(s) 713
AccII CGCG 1 cut(s) 261
AccIII TCCGGA 1 cut(s) 651
AciI CCGC 2 cut(s) 400, 711
AclWI GGATC 6 cut(s) 138, 151, 163, 676, 762, 775
AcoI YGGCCR 2 cut(s) 95, 437
AcsI RAATTY 3 cut(s) 44, 290, 349
AcuI CTGAAG 1 cut(s) 749
AfaI GTAC 1 cut(s) 332
AfiI CCNNNNNNNGG 1 cut(s) 280
AflII CTTAAG 1 cut(s) 338
AflIII ACRYGT 1 cut(s) 31
AgsI TTSAA 3 cut(s) 51, 83, 671
AjnI CCWGG 1 cut(s) 949
AluBI AGCT 9 cut(s) 56, 86, 245, 302, 435, 499, 589, 674, 896
AluI AGCT 9 cut(s) 56, 86, 245, 302, 435, 499, 589, 674, 896
Alw26I GTCTC 1 cut(s) 664
AlwI GGATC 6 cut(s) 138, 151, 163, 676, 762, 775
Aor13HI TCCGGA 1 cut(s) 651
AoxI GGCC 3 cut(s) 95, 223, 437
ApeKI GCWGC 7 cut(s) 296, 299, 499, 522, 586, 812, 815
ApoI RAATTY 3 cut(s) 44, 290, 349
AspLEI GCGC 2 cut(s) 263, 866
AspS9I GGNCC 3 cut(s) 112, 224, 649
AsuC2I CCSGG 2 cut(s) 148, 765
AsuHPI GGTGA 1 cut(s) 581
AsuNHI GCTAGC 1 cut(s) 431
AvaII GGWCC 2 cut(s) 112, 649
BalI TGGCCA 2 cut(s) 97, 439
BamHI GGATCC 2 cut(s) 143, 767
BauI CACGAG 2 cut(s) 210, 714
BbvI GCAGC 7 cut(s) 283, 311, 486, 534, 598, 799, 827
BccI CCATC 3 cut(s) 215, 512, 887
BciT130I CCWGG 1 cut(s) 951
BciVI GTATCC 2 cut(s) 407, 538
BclI TGATCA 1 cut(s) 571
BcnI CCSGG 2 cut(s) 148, 765
BcoDI GTCTC 1 cut(s) 664
BfaI CTAG 5 cut(s) 140, 158, 432, 503, 722
BfmI CTRYAG 3 cut(s) 297, 584, 813
BfrI CTTAAG 1 cut(s) 338
BfuAI ACCTGC 1 cut(s) 342
BfuI GTATCC 2 cut(s) 407, 538
BglII AGATCT 1 cut(s) 724
BisI GCNGC 8 cut(s) 297, 300, 500, 523, 587, 711, 813, 816
BlsI GCNGC 8 cut(s) 298, 301, 501, 524, 588, 712, 814, 817
Bme1390I CCNGG 3 cut(s) 148, 765, 951
Bme18I GGWCC 2 cut(s) 112, 649
BmgT120I GGNCC 3 cut(s) 112, 224, 649
BmiI GGNNCC 3 cut(s) 145, 650, 769
BmrFI CCNGG 3 cut(s) 148, 765, 951
BmrI ACTGGG 1 cut(s) 938
BmsI GCATC 2 cut(s) 144, 851
BmtI GCTAGC 1 cut(s) 435
BmuI ACTGGG 1 cut(s) 938
BpuEI CTTGAG 2 cut(s) 72, 872
BpuMI CCSGG 2 cut(s) 148, 765
BsaBI GATNNNNATC 2 cut(s) 562, 723
BsaWI WCCGGW 2 cut(s) 323, 651
Bsc4I CCNNNNNNNGG 1 cut(s) 280
Bse1I ACTGG 1 cut(s) 933
Bse8I GATNNNNATC 2 cut(s) 562, 723
BseAI TCCGGA 1 cut(s) 651
BseBI CCWGG 1 cut(s) 951
BseGI GGATG 4 cut(s) 523, 563, 739, 866
BseJI GATNNNNATC 2 cut(s) 562, 723
BseLI CCNNNNNNNGG 1 cut(s) 280
BseMII CTCAG 1 cut(s) 654
BseNI ACTGG 1 cut(s) 933
BseXI GCAGC 7 cut(s) 283, 311, 486, 534, 598, 799, 827
BseYI CCCAGC 1 cut(s) 195
Bsh1236I CGCG 1 cut(s) 261
BshFI GGCC 3 cut(s) 97, 225, 439
BsiSI CCGG 4 cut(s) 147, 324, 652, 764
BslFI GGGAC 1 cut(s) 885
BslI CCNNNNNNNGG 1 cut(s) 280
BsmAI GTCTC 1 cut(s) 664
BsmFI GGGAC 1 cut(s) 885
BsnI GGCC 3 cut(s) 97, 225, 439
Bsp13I TCCGGA 1 cut(s) 651
Bsp143I GATC 9 cut(s) 143, 168, 206, 571, 576, 681, 718, 724, 767
BspACI CCGC 2 cut(s) 400, 711
BspANI GGCC 3 cut(s) 97, 225, 439
BspCNI CTCAG 1 cut(s) 655
BspEI TCCGGA 1 cut(s) 651
BspFNI CGCG 1 cut(s) 261
BspHI TCATGA 1 cut(s) 622
BspLI GGNNCC 3 cut(s) 145, 650, 769
BspMAI CTGCAG 3 cut(s) 301, 588, 817
BspMI ACCTGC 1 cut(s) 342
BspOI GCTAGC 1 cut(s) 435
BspPI GGATC 6 cut(s) 138, 151, 163, 676, 762, 775
BspTI CTTAAG 1 cut(s) 338
BsrBI CCGCTC 1 cut(s) 713
BsrI ACTGG 1 cut(s) 933
BssMI GATC 9 cut(s) 143, 168, 206, 571, 576, 681, 718, 724, 767
BssSI CACGAG 2 cut(s) 210, 714
Bst2BI CACGAG 2 cut(s) 210, 714
Bst2UI CCWGG 1 cut(s) 951
Bst4CI ACNGT 3 cut(s) 116, 410, 602
Bst6I CTCTTC 1 cut(s) 438
BstAFI CTTAAG 1 cut(s) 338
BstAPI GCANNNNNTGC 1 cut(s) 293
BstC8I GCNNGC 5 cut(s) 433, 437, 483, 609, 676
BstDEI CTNAG 2 cut(s) 403, 663
BstF5I GGATG 4 cut(s) 523, 563, 739, 866
BstFNI CGCG 1 cut(s) 261
BstHHI GCGC 2 cut(s) 263, 866
BstKTI GATC 9 cut(s) 146, 171, 209, 574, 579, 684, 721, 727, 770
BstMAI GTCTC 1 cut(s) 664
BstMBI GATC 9 cut(s) 143, 168, 206, 571, 576, 681, 718, 724, 767
BstMWI GCNNNNNNNGC 2 cut(s) 293, 863
BstNI CCWGG 1 cut(s) 951
BstNSI RCATGY 3 cut(s) 35, 388, 485
BstSCI CCNGG 3 cut(s) 146, 763, 949
BstSFI CTRYAG 3 cut(s) 297, 584, 813
BstUI CGCG 1 cut(s) 261
BstV1I GCAGC 7 cut(s) 283, 311, 486, 534, 598, 799, 827
BstX2I RGATCY 4 cut(s) 143, 681, 724, 767
BstYI RGATCY 4 cut(s) 143, 681, 724, 767
BsuI GTATCC 2 cut(s) 407, 538
BsuRI GGCC 3 cut(s) 97, 225, 439
BtsCI GGATG 4 cut(s) 523, 563, 739, 866
BveI ACCTGC 1 cut(s) 342
Cac8I GCNNGC 5 cut(s) 433, 437, 483, 609, 676
CciI TCATGA 1 cut(s) 622
CfoI GCGC 2 cut(s) 263, 866
Cfr13I GGNCC 3 cut(s) 112, 224, 649
Csp6I GTAC 1 cut(s) 331
CviAII CATG 7 cut(s) 32, 185, 344, 385, 482, 541, 623
CviQI GTAC 1 cut(s) 331
DdeI CTNAG 2 cut(s) 403, 663
DpnI GATC 9 cut(s) 145, 170, 208, 573, 578, 683, 720, 726, 769
DpnII GATC 9 cut(s) 143, 168, 206, 571, 576, 681, 718, 724, 767
EaeI YGGCCR 2 cut(s) 95, 437
Eam1104I CTCTTC 1 cut(s) 438
EarI CTCTTC 1 cut(s) 438
Eco47I GGWCC 2 cut(s) 112, 649
Eco57I CTGAAG 1 cut(s) 749
EcoRII CCWGG 1 cut(s) 949
FaeI CATG 7 cut(s) 35, 188, 347, 388, 485, 544, 626
FaqI GGGAC 1 cut(s) 885
FatI CATG 7 cut(s) 31, 184, 343, 384, 481, 540, 622
FbaI TGATCA 1 cut(s) 571
Fnu4HI GCNGC 8 cut(s) 297, 300, 500, 523, 587, 711, 813, 816
FokI GGATG 4 cut(s) 530, 570, 746, 873
Fsp4HI GCNGC 8 cut(s) 297, 300, 500, 523, 587, 711, 813, 816
FspBI CTAG 5 cut(s) 140, 158, 432, 503, 722
FspI TGCGCA 1 cut(s) 865
GlaI GCGC 2 cut(s) 262, 865
GluI GCNGC 8 cut(s) 297, 300, 500, 523, 587, 711, 813, 816
GsaI CCCAGC 1 cut(s) 199
HaeIII GGCC 3 cut(s) 97, 225, 439
HapII CCGG 4 cut(s) 147, 324, 652, 764
HhaI GCGC 2 cut(s) 263, 866
Hin1II CATG 7 cut(s) 35, 188, 347, 388, 485, 544, 626
Hin6I GCGC 2 cut(s) 261, 864
HinP1I GCGC 2 cut(s) 261, 864
HinfI GANTC 1 cut(s) 563
HpaII CCGG 4 cut(s) 147, 324, 652, 764
HphI GGTGA 1 cut(s) 581
Hpy166II GTNNAC 2 cut(s) 190, 787
Hpy188I TCNGA 6 cut(s) 168, 360, 576, 664, 729, 916
Hpy188III TCNNGA 7 cut(s) 26, 623, 638, 652, 716, 722, 889
Hpy8I GTNNAC 2 cut(s) 190, 787
HpyAV CCTTC 2 cut(s) 482, 724
HpyCH4III ACNGT 3 cut(s) 116, 410, 602
HpyCH4V TGCA 9 cut(s) 176, 287, 299, 388, 485, 586, 594, 698, 815
HpyF10VI GCNNNNNNNGC 2 cut(s) 293, 863
HpyF3I CTNAG 2 cut(s) 403, 663
Hsp92II CATG 7 cut(s) 35, 188, 347, 388, 485, 544, 626
HspAI GCGC 2 cut(s) 261, 864
Kpn2I TCCGGA 1 cut(s) 651
Ksp22I TGATCA 1 cut(s) 571
Kzo9I GATC 9 cut(s) 143, 168, 206, 571, 576, 681, 718, 724, 767
LmnI GCTCC 1 cut(s) 250
Lsp1109I GCAGC 7 cut(s) 283, 311, 486, 534, 598, 799, 827
LweI GCATC 2 cut(s) 144, 851
MaeI CTAG 5 cut(s) 140, 158, 432, 503, 722
MaeIII GTNAC 3 cut(s) 116, 380, 940
MalI GATC 9 cut(s) 145, 170, 208, 573, 578, 683, 720, 726, 769
MbiI CCGCTC 1 cut(s) 713
MboI GATC 9 cut(s) 143, 168, 206, 571, 576, 681, 718, 724, 767
MboII GAAGA 2 cut(s) 163, 455
MflI RGATCY 4 cut(s) 143, 681, 724, 767
MlsI TGGCCA 2 cut(s) 97, 439
MluCI AATT 8 cut(s) 44, 78, 177, 290, 349, 389, 751, 917
MluNI TGGCCA 2 cut(s) 97, 439
MmeI TCCRAC 2 cut(s) 272, 298
MnlI CCTC 5 cut(s) 103, 131, 274, 439, 634
Mox20I TGGCCA 2 cut(s) 97, 439
MroI TCCGGA 1 cut(s) 651
MscI TGGCCA 2 cut(s) 97, 439
MseI TTAA 2 cut(s) 312, 339
MslI CAYNNNNRTG 1 cut(s) 480
Msp20I TGGCCA 2 cut(s) 97, 439
MspCI CTTAAG 1 cut(s) 338
MspI CCGG 4 cut(s) 147, 324, 652, 764
MspR9I CCNGG 3 cut(s) 148, 765, 951
MvaI CCWGG 1 cut(s) 951
MvnI CGCG 1 cut(s) 261
MwoI GCNNNNNNNGC 2 cut(s) 293, 863
NciI CCSGG 2 cut(s) 148, 765
NdeII GATC 9 cut(s) 143, 168, 206, 571, 576, 681, 718, 724, 767
NheI GCTAGC 1 cut(s) 431
NlaIII CATG 7 cut(s) 35, 188, 347, 388, 485, 544, 626
NlaIV GGNNCC 3 cut(s) 145, 650, 769
NmuCI GTSAC 1 cut(s) 116
NsbI TGCGCA 1 cut(s) 865
NspI RCATGY 3 cut(s) 35, 388, 485
PaeI GCATGC 1 cut(s) 485
PagI TCATGA 1 cut(s) 622
PciI ACATGT 1 cut(s) 31
PfeI GAWTC 1 cut(s) 563
PfoI TCCNGGA 2 cut(s) 146, 763
PkrI GCNGC 8 cut(s) 298, 301, 501, 524, 588, 712, 814, 817
PscI ACATGT 1 cut(s) 31
Psp6I CCWGG 1 cut(s) 949
PspFI CCCAGC 1 cut(s) 195
PspGI CCWGG 1 cut(s) 949
PspN4I GGNNCC 3 cut(s) 145, 650, 769
PspPI GGNCC 3 cut(s) 112, 224, 649
PsrI GAACNNNNNNTAC 2 cut(s) 21, 53
PstI CTGCAG 3 cut(s) 301, 588, 817
PsuI RGATCY 4 cut(s) 143, 681, 724, 767
RsaI GTAC 1 cut(s) 332
RsaNI GTAC 1 cut(s) 331
RseI CAYNNNNRTG 1 cut(s) 480
SaqAI TTAA 2 cut(s) 312, 339
SatI GCNGC 8 cut(s) 297, 300, 500, 523, 587, 711, 813, 816
Sau3AI GATC 9 cut(s) 143, 168, 206, 571, 576, 681, 718, 724, 767
Sau96I GGNCC 3 cut(s) 112, 224, 649
ScrFI CCNGG 3 cut(s) 148, 765, 951
SfaNI GCATC 2 cut(s) 144, 851
SfcI CTRYAG 3 cut(s) 297, 584, 813
SinI GGWCC 2 cut(s) 112, 649
SmiMI CAYNNNNRTG 1 cut(s) 480
SmlI CTYRAG 3 cut(s) 87, 338, 887
SmoI CTYRAG 3 cut(s) 87, 338, 887
SphI GCATGC 1 cut(s) 485
Sse9I AATT 8 cut(s) 44, 78, 177, 290, 349, 389, 751, 917
SsiI CCGC 2 cut(s) 400, 711
SspMI CTAG 5 cut(s) 140, 158, 432, 503, 722
StyD4I CCNGG 3 cut(s) 146, 763, 949
TaaI ACNGT 3 cut(s) 116, 410, 602
TaqI TCGA 3 cut(s) 9, 67, 495
TasI AATT 8 cut(s) 44, 78, 177, 290, 349, 389, 751, 917
TauI GCSGC 1 cut(s) 713
TfiI GAWTC 1 cut(s) 563
Tru1I TTAA 2 cut(s) 312, 339
Tru9I TTAA 2 cut(s) 312, 339
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 7 cut(s) 296, 299, 499, 522, 586, 812, 815
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 4 cut(s) 360, 529, 611, 639
TspGWI ACGGA 1 cut(s) 611
Vha464I CTTAAG 1 cut(s) 338
VpaK11BI GGWCC 2 cut(s) 112, 649
XapI RAATTY 3 cut(s) 44, 290, 349
XbaI TCTAGA 1 cut(s) 721
XceI RCATGY 3 cut(s) 35, 388, 485
XspI CTAG 5 cut(s) 140, 158, 432, 503, 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.