pycom11g03170

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0018500
Physical Location & Seq
Reverse (-)
2436525 .. 2438871
2347 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g03170.1

Sequence Viewer

Length: 1053 bp
ATGGAGATCACTACTGAGGTCACCTCTTATACGCCCGAAGACGTCGAGGAGTATCGGCTACGGAGGGGAATCCGTGTTGTAGGCTGGGATGATATTCCAAACCCTATCAGGGCCTTCGATGACACTGGGTTTCCGGAAAATGTTGTACAAGAATTTTCAAAAGCTGGATTTGTTAGACCTACACCAATTCAAGCTCAAGTATGGCCAATGGATTTGGAGGACCATGACCTCATTGGTATTGCTCAACCAGGATCTGGGAAGATGCTAGCGTATCTGATCCCTGCAATTCTCCATGTAAACTCCCTGCCAAAACGATCTCGTGGTGGAGATGGCCCAATAGTATTGATTTTGACTCCCACAATTCAACGCGCTGAACAAATCCAACAGGAGGGTGCTAAATTTGCTGCAGCTTACAACATTAAAGTCTTATCGGGCTTGCACCTGCTAAAACGTGAAAATTATGGTCAGACTTGTGGAGGTGGGATTTTAATCGACACACTTAGTAGCTTGCTTGTTTTGTTAAAGAGCAAAAGTACGAACTTGAAAAGCGTCACATACCTTGTGTTGGATGGTGCTGATTGGATGCTATCAGACACGCATGAGTCTCAAATACGAAATATATTATCTGAGATTCGCTCAGACTGTCAAAAATCATTCTGGAGTACTGGCTGGCCAGAAGAGGTTGATAAACGGGCAAAGCTATTCCTTCACAACGCTTGCAAAGTTCAAATAGTTTCTCCAGATCTGAAAGTGACACCTCCGGTTGACCAAATTGTTTACGTCCTTACTGAAGATCAGAAATATAACAAGTTGGTCGCGCTGCTATATGGTATATCGGATGGCAGCAGAGTAGTGATATTCATGGATACAAAGAAAGGACGTGAATCACACGATCAGATCGCCCTGCAGCTTTGCACGGCCGGCCTGCCCGCAGCCTCGTTTCATGAAAACAAAAGTCAAGCTGAAAGGATCGGTATTGCCTCTGAGTTTCAAGCTGGCAAGGTCCCTATAATGATTGCAACAGATGTAGCTGCTCGTGTTCTAGATCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko05202 ko05205 map03040 map05202 map05205
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

351

Amino Acids

38.81

Weight (kDa)

5.86

Isoelectric Point (pI)

38.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 61 - 229 1.6e-23 DEAD/DEAH box helicase
Helicase_C PF00271 267 - 349 1.3e-09 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 450
AatII GACGTC 1 cut(s) 45
Acc36I ACCTGC 1 cut(s) 450
AccB7I CCANNNNNTGG 1 cut(s) 254
AccII CGCG 2 cut(s) 369, 818
AccIII TCCGGA 1 cut(s) 133
AciI CCGC 1 cut(s) 930
AclWI GGATC 3 cut(s) 259, 271, 977
AcoI YGGCCR 3 cut(s) 203, 671, 918
AcsI RAATTY 2 cut(s) 152, 398
AcuI CTGAAG 1 cut(s) 810
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 3 cut(s) 147, 535, 664
AfiI CCNNNNNNNGG 4 cut(s) 109, 254, 388, 565
AgsI TTSAA 6 cut(s) 159, 191, 365, 544, 728, 992
AjiI CACGTC 1 cut(s) 881
AjnI CCWGG 1 cut(s) 247
AluBI AGCT 9 cut(s) 164, 194, 410, 507, 700, 910, 962, 995, 1031
AluI AGCT 9 cut(s) 164, 194, 410, 507, 700, 910, 962, 995, 1031
Alw26I GTCTC 1 cut(s) 609
AlwI GGATC 3 cut(s) 259, 271, 977
AlwNI CAGNNNCTG 1 cut(s) 254
Aor13HI TCCGGA 1 cut(s) 133
AoxI GGCC 6 cut(s) 111, 203, 331, 671, 918, 922
ApeKI GCWGC 7 cut(s) 404, 407, 820, 843, 907, 932, 1031
ApoI RAATTY 2 cut(s) 152, 398
AspLEI GCGC 2 cut(s) 371, 820
AspS9I GGNCC 4 cut(s) 111, 220, 332, 1003
AsuHPI GGTGA 1 cut(s) 13
AsuNHI GCTAGC 1 cut(s) 265
AvaII GGWCC 2 cut(s) 220, 1003
BalI TGGCCA 2 cut(s) 205, 673
BauI CACGAG 2 cut(s) 318, 1035
BbsI GAAGAC 1 cut(s) 45
BbvI GCAGC 7 cut(s) 391, 419, 807, 855, 919, 944, 1018
BccI CCATC 3 cut(s) 323, 563, 833
BceAI ACGGC 1 cut(s) 933
BciT130I CCWGG 1 cut(s) 249
BciVI GTATCC 1 cut(s) 859
BcoDI GTCTC 1 cut(s) 609
BfaI CTAG 2 cut(s) 266, 1043
BfmI CTRYAG 2 cut(s) 405, 905
BfuAI ACCTGC 1 cut(s) 450
BfuI GTATCC 1 cut(s) 859
BglII AGATCT 1 cut(s) 742
BisI GCNGC 7 cut(s) 405, 408, 821, 844, 908, 933, 1032
BlsI GCNGC 7 cut(s) 406, 409, 822, 845, 909, 934, 1033
BmcAI AGTACT 1 cut(s) 664
Bme1390I CCNGG 1 cut(s) 249
Bme18I GGWCC 2 cut(s) 220, 1003
BmgBI CACGTC 1 cut(s) 881
BmgT120I GGNCC 4 cut(s) 111, 220, 332, 1003
BmiI GGNNCC 1 cut(s) 1005
BmrFI CCNGG 1 cut(s) 249
BmrI ACTGGG 1 cut(s) 135
BmsI GCATC 2 cut(s) 252, 573
BmtI GCTAGC 1 cut(s) 269
BmuI ACTGGG 1 cut(s) 135
BpiI GAAGAC 1 cut(s) 45
BplI GAGNNNNNCTC 4 cut(s) 8, 40, 620, 652
BpmI CTGGAG 2 cut(s) 679, 723
BpuEI CTTGAG 1 cut(s) 180
Bsa29I ATCGAT 1 cut(s) 1048
BsaBI GATNNNNATC 1 cut(s) 488
BsaHI GRCGYC 1 cut(s) 42
BsaWI WCCGGW 2 cut(s) 133, 760
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 254, 388, 565
Bse118I RCCGGY 1 cut(s) 920
Bse1I ACTGG 2 cut(s) 130, 670
Bse8I GATNNNNATC 1 cut(s) 488
BseAI TCCGGA 1 cut(s) 133
BseBI CCWGG 1 cut(s) 249
BseCI ATCGAT 1 cut(s) 1048
BseGI GGATG 4 cut(s) 94, 574, 588, 844
BseJI GATNNNNATC 1 cut(s) 488
BseLI CCNNNNNNNGG 4 cut(s) 109, 254, 388, 565
BseMII CTCAG 4 cut(s) 6, 618, 651, 975
BseNI ACTGG 2 cut(s) 130, 670
BseRI GAGGAG 1 cut(s) 62
BseX3I CGGCCG 1 cut(s) 918
BseXI GCAGC 7 cut(s) 391, 419, 807, 855, 919, 944, 1018
BseYI CCCAGC 1 cut(s) 84
Bsh1236I CGCG 2 cut(s) 369, 818
Bsh1285I CGRYCG 1 cut(s) 921
BshFI GGCC 6 cut(s) 113, 205, 333, 673, 920, 924
BshVI ATCGAT 1 cut(s) 1048
BsiEI CGRYCG 1 cut(s) 921
BsiSI CCGG 3 cut(s) 134, 761, 921
BslFI GGGAC 1 cut(s) 989
BslI CCNNNNNNNGG 4 cut(s) 109, 254, 388, 565
BsmAI GTCTC 1 cut(s) 609
BsmFI GGGAC 1 cut(s) 989
BsnI GGCC 6 cut(s) 113, 205, 333, 673, 920, 924
Bsp13I TCCGGA 1 cut(s) 133
Bsp1407I TGTACA 1 cut(s) 145
BspACI CCGC 1 cut(s) 930
BspANI GGCC 6 cut(s) 113, 205, 333, 673, 920, 924
BspCNI CTCAG 4 cut(s) 7, 619, 650, 976
BspDI ATCGAT 1 cut(s) 1048
BspEI TCCGGA 1 cut(s) 133
BspFNI CGCG 2 cut(s) 369, 818
BspHI TCATGA 1 cut(s) 943
BspLI GGNNCC 1 cut(s) 1005
BspMAI CTGCAG 2 cut(s) 409, 909
BspMI ACCTGC 1 cut(s) 450
BspOI GCTAGC 1 cut(s) 269
BspPI GGATC 3 cut(s) 259, 271, 977
BsrFI RCCGGY 1 cut(s) 920
BsrGI TGTACA 1 cut(s) 145
BsrI ACTGG 2 cut(s) 130, 670
BssAI RCCGGY 1 cut(s) 920
BssNI GRCGYC 1 cut(s) 42
BssSI CACGAG 2 cut(s) 318, 1035
Bst2BI CACGAG 2 cut(s) 318, 1035
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 1 cut(s) 644
Bst6I CTCTTC 1 cut(s) 672
BstACI GRCGYC 1 cut(s) 42
BstAUI TGTACA 1 cut(s) 145
BstC8I GCNNGC 9 cut(s) 267, 437, 509, 671, 718, 922, 926, 930, 997
BstDEI CTNAG 5 cut(s) 15, 500, 627, 637, 984
BstEII GGTNACC 1 cut(s) 19
BstF5I GGATG 4 cut(s) 94, 574, 588, 844
BstFNI CGCG 2 cut(s) 369, 818
BstHHI GCGC 2 cut(s) 371, 820
BstMAI GTCTC 1 cut(s) 609
BstMCI CGRYCG 1 cut(s) 921
BstMWI GCNNNNNNNGC 2 cut(s) 401, 921
BstNI CCWGG 1 cut(s) 249
BstPI GGTNACC 1 cut(s) 19
BstSCI CCNGG 1 cut(s) 247
BstSFI CTRYAG 2 cut(s) 405, 905
BstUI CGCG 2 cut(s) 369, 818
BstV1I GCAGC 7 cut(s) 391, 419, 807, 855, 919, 944, 1018
BstV2I GAAGAC 1 cut(s) 45
BstX2I RGATCY 2 cut(s) 251, 742
BstYI RGATCY 2 cut(s) 251, 742
BstZI CGGCCG 1 cut(s) 918
Bsu15I ATCGAT 1 cut(s) 1048
BsuI GTATCC 1 cut(s) 859
BsuRI GGCC 6 cut(s) 113, 205, 333, 673, 920, 924
BsuTUI ATCGAT 1 cut(s) 1048
BtrI CACGTC 1 cut(s) 881
BtsCI GGATG 4 cut(s) 94, 574, 588, 844
BtsIMutI CAGTG 1 cut(s) 123
BveI ACCTGC 1 cut(s) 450
Cac8I GCNNGC 9 cut(s) 267, 437, 509, 671, 718, 922, 926, 930, 997
CaiI CAGNNNCTG 1 cut(s) 254
CciI TCATGA 1 cut(s) 943
CfoI GCGC 2 cut(s) 371, 820
Cfr10I RCCGGY 1 cut(s) 920
Cfr13I GGNCC 4 cut(s) 111, 220, 332, 1003
ClaI ATCGAT 1 cut(s) 1048
CseI GACGC 1 cut(s) 538
Csp6I GTAC 3 cut(s) 146, 534, 663
CviAII CATG 5 cut(s) 224, 293, 599, 862, 944
CviQI GTAC 3 cut(s) 146, 534, 663
DdeI CTNAG 5 cut(s) 15, 500, 627, 637, 984
EaeI YGGCCR 3 cut(s) 203, 671, 918
EagI CGGCCG 1 cut(s) 918
Eam1104I CTCTTC 1 cut(s) 672
EarI CTCTTC 1 cut(s) 672
EclXI CGGCCG 1 cut(s) 918
Eco47I GGWCC 2 cut(s) 220, 1003
Eco52I CGGCCG 1 cut(s) 918
Eco57I CTGAAG 1 cut(s) 810
Eco91I GGTNACC 1 cut(s) 19
EcoO109I RGGNCCY 2 cut(s) 111, 1003
EcoO65I GGTNACC 1 cut(s) 19
EcoRII CCWGG 1 cut(s) 247
FaeI CATG 5 cut(s) 227, 296, 602, 865, 947
FaqI GGGAC 1 cut(s) 989
FatI CATG 5 cut(s) 223, 292, 598, 861, 943
FauI CCCGC 1 cut(s) 937
Fnu4HI GCNGC 7 cut(s) 405, 408, 821, 844, 908, 933, 1032
FokI GGATG 4 cut(s) 101, 581, 595, 851
FseI GGCCGGCC 1 cut(s) 924
Fsp4HI GCNGC 7 cut(s) 405, 408, 821, 844, 908, 933, 1032
FspBI CTAG 2 cut(s) 266, 1043
GlaI GCGC 2 cut(s) 370, 819
GluI GCNGC 7 cut(s) 405, 408, 821, 844, 908, 933, 1032
GsaI CCCAGC 1 cut(s) 88
GsuI CTGGAG 2 cut(s) 679, 723
HaeIII GGCC 6 cut(s) 113, 205, 333, 673, 920, 924
HapII CCGG 3 cut(s) 134, 761, 921
HgaI GACGC 1 cut(s) 538
HhaI GCGC 2 cut(s) 371, 820
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 5 cut(s) 227, 296, 602, 865, 947
Hin6I GCGC 2 cut(s) 369, 818
HinP1I GCGC 2 cut(s) 369, 818
HincII GTYRAC 1 cut(s) 766
HindII GTYRAC 1 cut(s) 766
HinfI GANTC 5 cut(s) 69, 352, 602, 631, 884
HpaII CCGG 3 cut(s) 134, 761, 921
HphI GGTGA 1 cut(s) 13
Hpy166II GTNNAC 3 cut(s) 298, 766, 778
Hpy188III TCNNGA 5 cut(s) 134, 658, 740, 944, 1043
Hpy8I GTNNAC 3 cut(s) 298, 766, 778
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 2 cut(s) 124, 716
HpyCH4III ACNGT 1 cut(s) 644
HpyCH4IV ACGT 4 cut(s) 42, 451, 780, 880
HpyCH4V TGCA 7 cut(s) 284, 407, 439, 720, 907, 915, 1019
HpyF10VI GCNNNNNNNGC 2 cut(s) 401, 921
HpyF3I CTNAG 5 cut(s) 15, 500, 627, 637, 984
HpySE526I ACGT 4 cut(s) 42, 451, 780, 880
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 5 cut(s) 227, 296, 602, 865, 947
HspAI GCGC 2 cut(s) 369, 818
Kpn2I TCCGGA 1 cut(s) 133
KroI GCCGGC 1 cut(s) 920
KroNI GCCGGC 1 cut(s) 922
Lsp1109I GCAGC 7 cut(s) 391, 419, 807, 855, 919, 944, 1018
LweI GCATC 2 cut(s) 252, 573
MaeI CTAG 2 cut(s) 266, 1043
MaeII ACGT 4 cut(s) 42, 451, 780, 880
MaeIII GTNAC 3 cut(s) 19, 550, 751
MboII GAAGA 4 cut(s) 50, 271, 689, 803
MflI RGATCY 2 cut(s) 251, 742
MlsI TGGCCA 2 cut(s) 205, 673
MluCI AATT 7 cut(s) 152, 186, 285, 360, 398, 457, 771
MluNI TGGCCA 2 cut(s) 205, 673
MlyI GAGTC 2 cut(s) 346, 611
MmeI TCCRAC 2 cut(s) 406, 546
Mox20I TGGCCA 2 cut(s) 205, 673
MroI TCCGGA 1 cut(s) 133
MroNI GCCGGC 1 cut(s) 920
MscI TGGCCA 2 cut(s) 205, 673
MseI TTAA 3 cut(s) 420, 488, 521
Msp20I TGGCCA 2 cut(s) 205, 673
MspI CCGG 3 cut(s) 134, 761, 921
MspR9I CCNGG 1 cut(s) 249
MvaI CCWGG 1 cut(s) 249
MvnI CGCG 2 cut(s) 369, 818
MwoI GCNNNNNNNGC 2 cut(s) 401, 921
NaeI GCCGGC 1 cut(s) 922
NgoMIV GCCGGC 1 cut(s) 920
NheI GCTAGC 1 cut(s) 265
NlaIII CATG 5 cut(s) 227, 296, 602, 865, 947
NlaIV GGNNCC 1 cut(s) 1005
NmuCI GTSAC 3 cut(s) 19, 550, 751
PagI TCATGA 1 cut(s) 943
PaqCI CACCTGC 1 cut(s) 450
PdiI GCCGGC 1 cut(s) 922
PfeI GAWTC 3 cut(s) 69, 631, 884
PflMI CCANNNNNTGG 1 cut(s) 254
PkrI GCNGC 7 cut(s) 406, 409, 822, 845, 909, 934, 1033
PleI GAGTC 2 cut(s) 346, 610
PpsI GAGTC 2 cut(s) 346, 610
PpuMI RGGWCCY 1 cut(s) 1003
Psp5II RGGWCCY 1 cut(s) 1003
Psp6I CCWGG 1 cut(s) 247
PspEI GGTNACC 1 cut(s) 19
PspFI CCCAGC 1 cut(s) 84
PspGI CCWGG 1 cut(s) 247
PspN4I GGNNCC 1 cut(s) 1005
PspPI GGNCC 4 cut(s) 111, 220, 332, 1003
PspPPI RGGWCCY 1 cut(s) 1003
PstI CTGCAG 2 cut(s) 409, 909
PstNI CAGNNNCTG 1 cut(s) 254
PsuI RGATCY 2 cut(s) 251, 742
RigI GGCCGGCC 1 cut(s) 924
RsaI GTAC 3 cut(s) 147, 535, 664
RsaNI GTAC 3 cut(s) 146, 534, 663
SaqAI TTAA 3 cut(s) 420, 488, 521
SatI GCNGC 7 cut(s) 405, 408, 821, 844, 908, 933, 1032
Sau96I GGNCC 4 cut(s) 111, 220, 332, 1003
ScaI AGTACT 1 cut(s) 664
SchI GAGTC 2 cut(s) 346, 611
ScrFI CCNGG 1 cut(s) 249
SfaNI GCATC 2 cut(s) 252, 573
SfcI CTRYAG 2 cut(s) 405, 905
SinI GGWCC 2 cut(s) 220, 1003
SmlI CTYRAG 1 cut(s) 195
SmoI CTYRAG 1 cut(s) 195
Sse9I AATT 7 cut(s) 152, 186, 285, 360, 398, 457, 771
SsiI CCGC 1 cut(s) 930
SspMI CTAG 2 cut(s) 266, 1043
StyD4I CCNGG 1 cut(s) 247
TaaI ACNGT 1 cut(s) 644
TaiI ACGT 4 cut(s) 45, 454, 783, 883
TaqI TCGA 4 cut(s) 45, 117, 492, 1048
TasI AATT 7 cut(s) 152, 186, 285, 360, 398, 457, 771
TatI WGTACW 2 cut(s) 145, 662
TfiI GAWTC 3 cut(s) 69, 631, 884
Tru1I TTAA 3 cut(s) 420, 488, 521
Tru9I TTAA 3 cut(s) 420, 488, 521
TscAI CASTG 1 cut(s) 130
TseFI GTSAC 3 cut(s) 19, 550, 751
TseI GCWGC 7 cut(s) 404, 407, 820, 843, 907, 932, 1031
Tsp45I GTSAC 3 cut(s) 19, 550, 751
TspDTI ATGAA 3 cut(s) 850, 932, 960
TspGWI ACGGA 2 cut(s) 62, 76
TspRI CASTG 1 cut(s) 130
Van91I CCANNNNNTGG 1 cut(s) 254
VpaK11BI GGWCC 2 cut(s) 220, 1003
XapI RAATTY 2 cut(s) 152, 398
XbaI TCTAGA 1 cut(s) 1042
XcmI CCANNNNNNNNNTGG 1 cut(s) 230
XspI CTAG 2 cut(s) 266, 1043
ZraI GACGTC 1 cut(s) 43
ZrmI AGTACT 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.