MD11G1037800.v1.1

DEAD-box ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0018500
Physical Location & Seq
Reverse (-)
3232469 .. 3235257
2789 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1037800.v1.1.491

Sequence Viewer

Length: 1482 bp
ATGGCGAAGCGGTCCAATGTCCCTAATTCAGTAAGCCTAACACAACATTATGGTGCTGATGCCGCTCAAGGCTCCTCAAATACGGCCAGTGAGGGCAAGGAGTATGGGAGCCAACGGAGGAAAATTACTGCTCAAGGCGGCACGCCTGAAACGCAGGACCACCACAAGAGGGAAATTACTACTGATGGCACATCTTATGCGCCCGACGAAGTCGAGGAGTATAGGCTACGGAGGGAAATCCGTGTTGTAGGCTGGGATGATGTTCCGGACCCCATCACGACTTTCGATAACACTCGGTTTCCAGAACATGTTATACAAGAATTTTCAAAAGCTGGATTTGTTCGACCTACACCAATTCAAGCTCAAGTATGGCCAATGGATTTGGAGGACCGTGACCTTATTGGTATTGCTCAACCAGGATCCGGGAAGATGCTAGGGTATCTGATCCCTGCAATTTTCCATGTAAACTCCCAGCCAAAACGATCTAGTGGTGGAGATGGCCCAATAGTATTGATTTTAGCTCTAACAACTCAACGCGCTGAACAAATCCAACAGGAGGGTGCTAAATTTGCTGCAGCTTACAACATTAAAGTCTTATCCGGCTTGTACCTGCTTAAGCATGAAAATTCTGGTCAGACTTGTGGCATTGGTTATGGTAACATGCAATTTTGTGTAGGAGGTGGGATTTTAATCGACACACTTAGTAGCTTGCTTGTTTTGTTAAAGAGCAAAAGTACGAACTTGAAAAGCGTTACATACCTTGTGTTGGATGGTGCTGATTGGATGCTATCAGACACGCATGAGCCTCAAATTCGAAATATATTATCTCAGATCTGTTTAGACTATCAAAAATCATTCTGGGGTGCTAGCTGGCTAGAAGAGGTTGATAAACGGGCAAAACTATTCCTTCACAACGCATGCAAAGTTCAAATAGTTTCTCCAATTCTGAAAGTGACACCTCCGGTCAACCAAATTGTTTACGTCCTTACTGAAGATCAGAAATATAACAAGTTGGTCGAGCTGCTAGGTGGTATATTGGATGGCAGCAGAATAGTGATATTCATGGATACAAAGAAAGGATGTGAATCACGTGATCAGATCGCCCTGCAGCTTTGCACGGACGGTCAGCACGTAGTCTTGGTTCATGAAAACGAAAGTCAAGCGGAAAGGGTCCGGATTGTCTCTGAGTTTCAAGCTGGCAAGATCCCCATAATGATTGCAACAGATGTTGCCGCTCGTGATCTAGATCTGAAGGATGTGAAATATGTTATCAATTACGACTTTCCGGGATCCCTTGATGATTATGTTTACCACATTGACTGCTTTGGAAGTGCTGCAGCAAAAGGAACAGCATACACATTCTTTACAACTGCTAATGCTGGATGCGCAAAGGGACTTACCATCTTACTCAAGAAAGCTGGACAGAATGTTAGTTCTGAATTGGAACAACTGGGGCGTGATGTAACATATCCAGGTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 ko05202 ko05205 map03040 map05202 map05205
Pfam Domains
Protein Families
DEAD Helicase_C

Protein Analysis

494

Amino Acids

54.35

Weight (kDa)

5.82

Isoelectric Point (pI)

37.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 117 - 283 4.6e-17 DEAD/DEAH box helicase
Helicase_C PF00271 334 - 436 3.3e-18 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1387
Acc36I ACCTGC 1 cut(s) 618
AccBSI CCGCTC 2 cut(s) 65, 1235
AccII CGCG 1 cut(s) 537
AccIII TCCGGA 2 cut(s) 265, 1173
AciI CCGC 5 cut(s) 10, 63, 138, 1163, 1233
AclWI GGATC 6 cut(s) 414, 427, 439, 1198, 1284, 1297
AcoI YGGCCR 2 cut(s) 84, 371
AcsI RAATTY 4 cut(s) 320, 566, 625, 810
AcuI CTGAAG 2 cut(s) 1011, 1271
AcvI CACGTG 1 cut(s) 1091
AfaI GTAC 2 cut(s) 608, 736
AfiI CCNNNNNNNGG 4 cut(s) 169, 422, 556, 766
AflII CTTAAG 1 cut(s) 614
AflIII ACRYGT 1 cut(s) 307
AgsI TTSAA 5 cut(s) 327, 359, 745, 929, 1193
AjnI CCWGG 2 cut(s) 415, 1471
Alw26I GTCTC 1 cut(s) 1186
AlwI GGATC 6 cut(s) 414, 427, 439, 1198, 1284, 1297
Aor13HI TCCGGA 2 cut(s) 265, 1173
AoxI GGCC 3 cut(s) 84, 371, 499
ApeKI GCWGC 7 cut(s) 572, 575, 1021, 1044, 1108, 1334, 1337
ApoI RAATTY 4 cut(s) 320, 566, 625, 810
AspLEI GCGC 3 cut(s) 202, 539, 1388
AspS9I GGNCC 6 cut(s) 12, 157, 268, 388, 500, 1171
AsuC2I CCSGG 2 cut(s) 424, 1287
AsuII TTCGAA 1 cut(s) 814
AsuNHI GCTAGC 1 cut(s) 866
AvaII GGWCC 5 cut(s) 12, 157, 268, 388, 1171
BalI TGGCCA 1 cut(s) 373
BamHI GGATCC 2 cut(s) 419, 1289
BauI CACGAG 1 cut(s) 1236
BbrPI CACGTG 1 cut(s) 1091
BbvI GCAGC 7 cut(s) 559, 587, 1008, 1056, 1120, 1321, 1349
BccI CCATC 6 cut(s) 179, 281, 491, 764, 1034, 1409
BceAI ACGGC 1 cut(s) 99
BciT130I CCWGG 2 cut(s) 417, 1473
BciVI GTATCC 1 cut(s) 1060
BclI TGATCA 1 cut(s) 1093
BcnI CCSGG 2 cut(s) 424, 1287
BcoDI GTCTC 1 cut(s) 1186
BfaI CTAG 6 cut(s) 434, 486, 867, 875, 1025, 1244
BfmI CTRYAG 3 cut(s) 573, 1106, 1335
BfrI CTTAAG 1 cut(s) 614
BfuAI ACCTGC 1 cut(s) 618
BfuI GTATCC 1 cut(s) 1060
BglII AGATCT 2 cut(s) 831, 1246
Bme1390I CCNGG 4 cut(s) 417, 424, 1287, 1473
Bme18I GGWCC 5 cut(s) 12, 157, 268, 388, 1171
BmgT120I GGNCC 6 cut(s) 12, 157, 268, 388, 500, 1171
BmiI GGNNCC 6 cut(s) 73, 110, 270, 421, 1172, 1291
BmrFI CCNGG 4 cut(s) 417, 424, 1287, 1473
BmrI ACTGGG 1 cut(s) 1460
BmsI GCATC 4 cut(s) 49, 420, 774, 1373
BmtI GCTAGC 1 cut(s) 870
BmuI ACTGGG 1 cut(s) 1460
Bpu14I TTCGAA 1 cut(s) 814
BpuEI CTTGAG 4 cut(s) 51, 117, 348, 1394
BpuMI CCSGG 2 cut(s) 424, 1287
BsaAI YACGTR 2 cut(s) 1091, 1132
BsaBI GATNNNNATC 3 cut(s) 689, 1084, 1245
BsaWI WCCGGW 3 cut(s) 265, 961, 1173
Bsc4I CCNNNNNNNGG 4 cut(s) 169, 422, 556, 766
Bse1I ACTGG 2 cut(s) 87, 1455
Bse8I GATNNNNATC 3 cut(s) 689, 1084, 1245
BseAI TCCGGA 2 cut(s) 265, 1173
BseBI CCWGG 2 cut(s) 417, 1473
BseGI GGATG 7 cut(s) 262, 775, 789, 1045, 1085, 1261, 1388
BseJI GATNNNNATC 3 cut(s) 689, 1084, 1245
BseLI CCNNNNNNNGG 4 cut(s) 169, 422, 556, 766
BseMII CTCAG 2 cut(s) 842, 1176
BseNI ACTGG 2 cut(s) 87, 1455
BseRI GAGGAG 2 cut(s) 64, 230
BseXI GCAGC 7 cut(s) 559, 587, 1008, 1056, 1120, 1321, 1349
BseYI CCCAGC 2 cut(s) 252, 471
Bsh1236I CGCG 1 cut(s) 537
BshFI GGCC 3 cut(s) 86, 373, 501
BsiSI CCGG 6 cut(s) 266, 423, 600, 962, 1174, 1286
BslFI GGGAC 2 cut(s) 5, 1407
BslI CCNNNNNNNGG 4 cut(s) 169, 422, 556, 766
BsmAI GTCTC 1 cut(s) 1186
BsmFI GGGAC 2 cut(s) 5, 1407
BsnI GGCC 3 cut(s) 86, 373, 501
Bsp119I TTCGAA 1 cut(s) 814
Bsp13I TCCGGA 2 cut(s) 265, 1173
BspACI CCGC 5 cut(s) 10, 63, 138, 1163, 1233
BspANI GGCC 3 cut(s) 86, 373, 501
BspCNI CTCAG 2 cut(s) 841, 1177
BspEI TCCGGA 2 cut(s) 265, 1173
BspFNI CGCG 1 cut(s) 537
BspHI TCATGA 1 cut(s) 1144
BspLI GGNNCC 6 cut(s) 73, 110, 270, 421, 1172, 1291
BspMAI CTGCAG 3 cut(s) 577, 1110, 1339
BspMI ACCTGC 1 cut(s) 618
BspOI GCTAGC 1 cut(s) 870
BspPI GGATC 6 cut(s) 414, 427, 439, 1198, 1284, 1297
BspT104I TTCGAA 1 cut(s) 814
BspTI CTTAAG 1 cut(s) 614
BsrBI CCGCTC 2 cut(s) 65, 1235
BsrI ACTGG 2 cut(s) 87, 1455
BssSI CACGAG 1 cut(s) 1236
Bst2BI CACGAG 1 cut(s) 1236
Bst2UI CCWGG 2 cut(s) 417, 1473
Bst4CI ACNGT 2 cut(s) 392, 1124
Bst6I CTCTTC 1 cut(s) 873
BstAFI CTTAAG 1 cut(s) 614
BstBAI YACGTR 2 cut(s) 1091, 1132
BstBI TTCGAA 1 cut(s) 814
BstC8I GCNNGC 6 cut(s) 143, 710, 868, 872, 919, 1198
BstDEI CTNAG 3 cut(s) 701, 828, 1185
BstF5I GGATG 7 cut(s) 262, 775, 789, 1045, 1085, 1261, 1388
BstFNI CGCG 1 cut(s) 537
BstHHI GCGC 3 cut(s) 202, 539, 1388
BstMAI GTCTC 1 cut(s) 1186
BstMWI GCNNNNNNNGC 4 cut(s) 62, 151, 569, 1385
BstNI CCWGG 2 cut(s) 417, 1473
BstNSI RCATGY 3 cut(s) 311, 664, 921
BstSCI CCNGG 4 cut(s) 415, 422, 1285, 1471
BstSFI CTRYAG 3 cut(s) 573, 1106, 1335
BstUI CGCG 1 cut(s) 537
BstV1I GCAGC 7 cut(s) 559, 587, 1008, 1056, 1120, 1321, 1349
BstX2I RGATCY 5 cut(s) 419, 831, 1203, 1246, 1289
BstYI RGATCY 5 cut(s) 419, 831, 1203, 1246, 1289
BsuI GTATCC 1 cut(s) 1060
BsuRI GGCC 3 cut(s) 86, 373, 501
BtsCI GGATG 7 cut(s) 262, 775, 789, 1045, 1085, 1261, 1388
BtsIMutI CAGTG 1 cut(s) 94
BveI ACCTGC 1 cut(s) 618
Cac8I GCNNGC 6 cut(s) 143, 710, 868, 872, 919, 1198
CciI TCATGA 1 cut(s) 1144
CfoI GCGC 3 cut(s) 202, 539, 1388
Cfr13I GGNCC 6 cut(s) 12, 157, 268, 388, 500, 1171
Csp6I GTAC 2 cut(s) 607, 735
CviAII CATG 8 cut(s) 308, 461, 620, 661, 800, 918, 1063, 1145
CviQI GTAC 2 cut(s) 607, 735
DdeI CTNAG 3 cut(s) 701, 828, 1185
EaeI YGGCCR 2 cut(s) 84, 371
Eam1104I CTCTTC 1 cut(s) 873
EarI CTCTTC 1 cut(s) 873
Eco47I GGWCC 5 cut(s) 12, 157, 268, 388, 1171
Eco57I CTGAAG 2 cut(s) 1011, 1271
Eco72I CACGTG 1 cut(s) 1091
EcoRII CCWGG 2 cut(s) 415, 1471
FaeI CATG 8 cut(s) 311, 464, 623, 664, 803, 921, 1066, 1148
FaqI GGGAC 2 cut(s) 5, 1407
FatI CATG 8 cut(s) 307, 460, 619, 660, 799, 917, 1062, 1144
FbaI TGATCA 1 cut(s) 1093
FokI GGATG 7 cut(s) 269, 782, 796, 1052, 1092, 1268, 1395
FspBI CTAG 6 cut(s) 434, 486, 867, 875, 1025, 1244
FspI TGCGCA 1 cut(s) 1387
GlaI GCGC 3 cut(s) 201, 538, 1387
GsaI CCCAGC 2 cut(s) 256, 475
HaeIII GGCC 3 cut(s) 86, 373, 501
HapII CCGG 6 cut(s) 266, 423, 600, 962, 1174, 1286
HhaI GCGC 3 cut(s) 202, 539, 1388
Hin1II CATG 8 cut(s) 311, 464, 623, 664, 803, 921, 1066, 1148
Hin6I GCGC 3 cut(s) 200, 537, 1386
HinP1I GCGC 3 cut(s) 200, 537, 1386
HincII GTYRAC 1 cut(s) 967
HindII GTYRAC 1 cut(s) 967
HinfI GANTC 1 cut(s) 1085
HpaII CCGG 6 cut(s) 266, 423, 600, 962, 1174, 1286
Hpy166II GTNNAC 4 cut(s) 466, 967, 979, 1309
Hpy188III TCNNGA 8 cut(s) 266, 277, 302, 1145, 1174, 1238, 1244, 1411
Hpy8I GTNNAC 4 cut(s) 466, 967, 979, 1309
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 2 cut(s) 917, 1246
HpyCH4III ACNGT 2 cut(s) 392, 1124
HpyCH4IV ACGT 3 cut(s) 981, 1090, 1131
HpyCH4V TGCA 8 cut(s) 452, 575, 664, 921, 1108, 1116, 1220, 1337
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 151, 569, 1385
HpyF3I CTNAG 3 cut(s) 701, 828, 1185
HpySE526I ACGT 3 cut(s) 981, 1090, 1131
Hsp92II CATG 8 cut(s) 311, 464, 623, 664, 803, 921, 1066, 1148
HspAI GCGC 3 cut(s) 200, 537, 1386
Kpn2I TCCGGA 2 cut(s) 265, 1173
Ksp22I TGATCA 1 cut(s) 1093
LmnI GCTCC 2 cut(s) 77, 108
Lsp1109I GCAGC 7 cut(s) 559, 587, 1008, 1056, 1120, 1321, 1349
LweI GCATC 4 cut(s) 49, 420, 774, 1373
MaeI CTAG 6 cut(s) 434, 486, 867, 875, 1025, 1244
MaeII ACGT 3 cut(s) 981, 1090, 1131
MaeIII GTNAC 5 cut(s) 392, 656, 751, 952, 1462
MbiI CCGCTC 2 cut(s) 65, 1235
MboII GAAGA 3 cut(s) 439, 890, 1004
MflI RGATCY 5 cut(s) 419, 831, 1203, 1246, 1289
MlsI TGGCCA 1 cut(s) 373
MluNI TGGCCA 1 cut(s) 373
MmeI TCCRAC 2 cut(s) 574, 747
Mox20I TGGCCA 1 cut(s) 373
MroI TCCGGA 2 cut(s) 265, 1173
MscI TGGCCA 1 cut(s) 373
MseI TTAA 4 cut(s) 588, 615, 689, 722
MslI CAYNNNNRTG 1 cut(s) 51
Msp20I TGGCCA 1 cut(s) 373
MspCI CTTAAG 1 cut(s) 614
MspI CCGG 6 cut(s) 266, 423, 600, 962, 1174, 1286
MspR9I CCNGG 4 cut(s) 417, 424, 1287, 1473
MvaI CCWGG 2 cut(s) 417, 1473
MvnI CGCG 1 cut(s) 537
MwoI GCNNNNNNNGC 4 cut(s) 62, 151, 569, 1385
NciI CCSGG 2 cut(s) 424, 1287
NheI GCTAGC 1 cut(s) 866
NlaIII CATG 8 cut(s) 311, 464, 623, 664, 803, 921, 1066, 1148
NlaIV GGNNCC 6 cut(s) 73, 110, 270, 421, 1172, 1291
NmuCI GTSAC 2 cut(s) 392, 952
NsbI TGCGCA 1 cut(s) 1387
NspI RCATGY 3 cut(s) 311, 664, 921
NspV TTCGAA 1 cut(s) 814
PaeI GCATGC 1 cut(s) 921
PagI TCATGA 1 cut(s) 1144
PciI ACATGT 1 cut(s) 307
PcsI WCGNNNNNNNCGW 1 cut(s) 1128
PfeI GAWTC 1 cut(s) 1085
PflFI GACNNNGTC 1 cut(s) 209
PfoI TCCNGGA 2 cut(s) 422, 1285
PmaCI CACGTG 1 cut(s) 1091
PmlI CACGTG 1 cut(s) 1091
Ppu21I YACGTR 2 cut(s) 1091, 1132
PscI ACATGT 1 cut(s) 307
Psp6I CCWGG 2 cut(s) 415, 1471
PspCI CACGTG 1 cut(s) 1091
PspFI CCCAGC 2 cut(s) 252, 471
PspGI CCWGG 2 cut(s) 415, 1471
PspN4I GGNNCC 6 cut(s) 73, 110, 270, 421, 1172, 1291
PspPI GGNCC 6 cut(s) 12, 157, 268, 388, 500, 1171
PsrI GAACNNNNNNTAC 4 cut(s) 297, 329, 1125, 1157
PstI CTGCAG 3 cut(s) 577, 1110, 1339
PsuI RGATCY 5 cut(s) 419, 831, 1203, 1246, 1289
PsyI GACNNNGTC 1 cut(s) 209
RsaI GTAC 2 cut(s) 608, 736
RsaNI GTAC 2 cut(s) 607, 735
RseI CAYNNNNRTG 1 cut(s) 51
SaqAI TTAA 4 cut(s) 588, 615, 689, 722
Sau96I GGNCC 6 cut(s) 12, 157, 268, 388, 500, 1171
ScrFI CCNGG 4 cut(s) 417, 424, 1287, 1473
SfaNI GCATC 4 cut(s) 49, 420, 774, 1373
SfcI CTRYAG 3 cut(s) 573, 1106, 1335
SfuI TTCGAA 1 cut(s) 814
SinI GGWCC 5 cut(s) 12, 157, 268, 388, 1171
SmiMI CAYNNNNRTG 1 cut(s) 51
SmlI CTYRAG 5 cut(s) 66, 132, 363, 614, 1409
SmoI CTYRAG 5 cut(s) 66, 132, 363, 614, 1409
SphI GCATGC 1 cut(s) 921
SsiI CCGC 5 cut(s) 10, 63, 138, 1163, 1233
SspMI CTAG 6 cut(s) 434, 486, 867, 875, 1025, 1244
StyD4I CCNGG 4 cut(s) 415, 422, 1285, 1471
TaaI ACNGT 2 cut(s) 392, 1124
TaiI ACGT 3 cut(s) 984, 1093, 1134
TaqI TCGA 6 cut(s) 213, 285, 343, 693, 814, 1017
TauI GCSGC 3 cut(s) 65, 141, 1235
TfiI GAWTC 1 cut(s) 1085
Tru1I TTAA 4 cut(s) 588, 615, 689, 722
Tru9I TTAA 4 cut(s) 588, 615, 689, 722
TscAI CASTG 1 cut(s) 94
TseFI GTSAC 2 cut(s) 392, 952
TseI GCWGC 7 cut(s) 572, 575, 1021, 1044, 1108, 1334, 1337
Tsp45I GTSAC 2 cut(s) 392, 952
TspDTI ATGAA 4 cut(s) 636, 1051, 1133, 1161
TspGWI ACGGA 4 cut(s) 130, 230, 244, 1133
TspRI CASTG 1 cut(s) 94
Tth111I GACNNNGTC 1 cut(s) 209
Vha464I CTTAAG 1 cut(s) 614
VpaK11BI GGWCC 5 cut(s) 12, 157, 268, 388, 1171
XapI RAATTY 4 cut(s) 320, 566, 625, 810
XbaI TCTAGA 1 cut(s) 1243
XceI RCATGY 3 cut(s) 311, 664, 921
XspI CTAG 6 cut(s) 434, 486, 867, 875, 1025, 1244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.