Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0006701

Physical Location & Seq

Genomic Coordinates Reverse (-)

2959614 .. 2964167

Gene Structure 4554 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1037400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1866 bp

ATGCCACAAGAAAGCAATTTTAATTCAATAATCTTCACATCACCTAGCAAGAACATGAGAGGGCTGAAGGGTTTAGTCTCCAACAATGAAGCTCCTCCATTTCCAAACACTGAGGAGTTCATCAATGACTATGAATTGGCTCAAAGGAAAGCTGAAGAAGCAGCTTCAAGGAGATACCAAGCAGCAGAATGGTTGAGGAAGATGGACTATGGCGCTTCCGGAACACTTTCCAAAGAGCCCTCTGAAGAAGAGTTCCGCCTTGCTCTCCGCAATGGCCTTATTCTCTGCAACGTCCTCAACAAAGTCAATCCTGGTGCTGTTCTTAAGGTGGTGGAAAATCCCATCATTGCGGTTCAGTCAACGGAGGGGGCAGCACAGTCTGCAATTCAGTATTTTGAGAACATGAGGAATTTCCTTGAGGCTGTTAAGGACATGAAGCTCTTGACATTCGAAGCGTCTGATCTCGAAAAGGGAGGCTCGTCGAGTAAAGTTGTAGACTGCATTCTTTGTCTCAAAGGATTTTACGAATGGAAGCAAGCCGGTGGAATTGGAGTTTGGAGATATGGAGGGACTGTGAGGATCACATCCTTCCCAAAAGGGTCACTATCCTCCTTAGGTAGTGAAAGTGCTGATGAGTCAATAGACGAGTCGGAGTCATCACAGTTTGAACAACTACTGGAGTTTCTTCATCTTTCTAGTGAGGTCTCAACCGAAGAATCCAGAACTGCCAATGCTCTCGCTTTCCTTTTCGATCGCTTTGGACTTGGACTTATACAAGCTTACCTTCGAGAGACTAATATGGTCGAAGACTTGCCCTTGAATGCAACGGTAATCGATACTTTACTGAGTAAGGTAGTCAAGGATTTCTCAGCGCTGCTTGTTTCACAAGGCACTCAGGATGCCATTCGGCATCGCGTGGAAATAATCTTATATCCCTTATTTACAAATTTTCTGCAGCTTGGCCTCTTTCTAAAGAAACTACTGAGAGGAGACTTGGGTGGTCTATCAAAATCGGAATTTGTAGAAGCTATTACACAATATCTAGGTCAAAGAAATGGTTCGGTGTCAAATGATCTATCCAACTTCTGCATTTGTGGTGGGAAAGGTAGCGAGGCTGTTCAGTGTGATAGCAGTCATTCGTCTGTCCATGAAGAACTAATTAGGACTCAACAGAAACAGCTTGAGGAGCTCAAATCATCTTTCCAACTGACGAAATACAAAGTCAAACAAATTCATTCAAACTGGGAGGAAGAACTTAGAAGGCTTGCACATCACATCAAAGGCCTTGAAGTGGCATCCTCTTCCTATCAACAAGTTATCGAAGAGAACCGAGTTCTTTACAATCAAGTGCAAGACCTCAAAGGATCAATTAGAGTCTATTGCAGAATCAGACCCTTTTTACCGGGGCAATTGAACGGCCAGTCCACTGTGGATTATATCGGAGAGAATGGAACCATCATGATTGTCAATCCCCTTAAACAGGGCAAAGATGCAAGAAGGGTTTTTACATTTAACAAAGTCTTCAGAACAAATGTCACACAAGAGCAAATATATGGTGACACTCAACCACTGATCAGGTCGGTTTTAGACGGTTATAATGCATGCATCTTCGCGTATGGACAGACTGGTTCGGGGAAGACATACACAATGAGTGGCCCTGATTTAACAACAGAAGAGACATGGGGTGTAAACTATAGAGCCCTTCGTGACTTATTTCAAATATCGAAGGCGAGGCAAGACATAGTAAGATATGAAGTTGCAGTTCAGATGATCGAAATATACAACGAACAAGTGAGAGACCTTTTAGTCAGCGATGGCTCTAACAGAAGATATCCTTGCCCTTCCTTTATTTTTTCTCCAACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005856 GO:0005871 GO:0005875 GO:0006091 GO:0006928 GO:0007017 GO:0007018 GO:0007275 GO:0008150 GO:0008152 GO:0009060 GO:0009791 GO:0009845 GO:0009987 GO:0015630 GO:0015980 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032501 GO:0032502 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044464 GO:0045333 GO:0048856 GO:0055114 GO:0090351

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CH Kinesin

Protein Analysis

622

Amino Acids

69.74

Weight (kDa)

5.6

Isoelectric Point (pI)

42.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CH	PF00307	59 - 175	5.1e-10	Calponin homology (CH) domain
Microtub_bd	PF16796	442 - 602	7e-41	Microtubule binding
Kinesin	PF00225	462 - 605	2.7e-38	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006701)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44730 AT3G44730 AT3G44730 AT3G44730
fragaria_vesca	FvH4_3g42160
malus_domestica	MD03G1037300.v1.1 MD03G1037400.v1.1 MD11G1038400.v1.1 MD11G1038500.v1.1
prunus_persica	Prupe.6G029300_v2.0.a1 Prupe.6G029300_v2.0.a1 Prupe.6G029300_v2.0.a1 Prupe.6G029300_v2.0.a1
pyrus_communis	pycom03g02870 pycom11g03260
rosa_chinensis	RchiOBHm_Chr5g0075401 RchiOBHm_Chr5g0075421
rosa_laevigata	RLG00000036560
rosa_multiflora	Rmu_sc0000770.1_g000017
rosa_roxburghii	Rroxscaffold_1G00006100
rosa_rugosa	Rorug05G0439500
rosa_samantha	Rh5AG496100 Rh5BG517700 Rh5CG541300 Rh5DG531600
rosa_wichuraiana	Rw5G046030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1596
AasI	GACNNNNNNGTC	1 cut(s)	1805
AccI	GTMKAC	1 cut(s)	495
AccII	CGCG	2 cut(s)	915, 1613
AccIII	TCCGGA	1 cut(s)	218
AciI	CCGC	3 cut(s)	256, 268, 350
AclWI	GGATC	2 cut(s)	587, 1372
AcoI	YGGCCR	1 cut(s)	1417
AcsI	RAATTY	4 cut(s)	409, 946, 1016, 1230
AcuI	CTGAAG	4 cut(s)	86, 174, 264, 1507
AfeI	AGCGCT	1 cut(s)	873
AfiI	CCNNNNNNNGG	2 cut(s)	1291, 1480
AflII	CTTAAG	1 cut(s)	323
AgsI	TTSAA	8 cut(s)	27, 168, 668, 820, 1239, 1289, 1414, 1718
AjnI	CCWGG	1 cut(s)	310
AjuI	GAANNNNNNNTTGG	2 cut(s)	538, 570
AloI	GAACNNNNNNTCC	2 cut(s)	1406, 1438
AluBI	AGCT	9 cut(s)	92, 152, 164, 439, 779, 958, 1028, 1180, 1189
AluI	AGCT	9 cut(s)	92, 152, 164, 439, 779, 958, 1028, 1180, 1189
Alw21I	GWGCWC	1 cut(s)	1191
Alw26I	GTCTC	7 cut(s)	82, 515, 709, 785, 984, 1670, 1791
AlwI	GGATC	2 cut(s)	587, 1372
Aor13HI	TCCGGA	1 cut(s)	218
Aor51HI	AGCGCT	1 cut(s)	873
AoxI	GGCC	5 cut(s)	274, 961, 1282, 1417, 1654
ApeKI	GCWGC	5 cut(s)	161, 182, 371, 874, 955
ApoI	RAATTY	4 cut(s)	409, 946, 1016, 1230
Asp700I	GAANNNNTTC	2 cut(s)	226, 1057
AspLEI	GCGC	2 cut(s)	215, 874
AspS9I	GGNCC	1 cut(s)	1655
AsuC2I	CCSGG	1 cut(s)	1404
AsuHPI	GGTGA	2 cut(s)	33, 1568
AsuII	TTCGAA	1 cut(s)	450
AxyI	CCTNAGG	1 cut(s)	613
BanII	GRGCYC	3 cut(s)	240, 1191, 1702
BarI	GAAGNNNNNNTAC	2 cut(s)	1489, 1521
BbsI	GAAGAC	3 cut(s)	813, 1513, 1643
Bbv12I	GWGCWC	1 cut(s)	1191
BbvI	GCAGC	5 cut(s)	173, 194, 383, 861, 967
BccI	CCATC	4 cut(s)	196, 350, 1463, 1808
BceAI	ACGGC	1 cut(s)	1432
BciT130I	CCWGG	1 cut(s)	312
BclI	TGATCA	1 cut(s)	1572
BcnI	CCSGG	1 cut(s)	1404
BcoDI	GTCTC	7 cut(s)	82, 515, 709, 785, 984, 1670, 1791
BfaI	CTAG	3 cut(s)	45, 696, 1043
BfmI	CTRYAG	2 cut(s)	953, 1693
BfoI	RGCGCY	2 cut(s)	216, 875
BfrI	CTTAAG	1 cut(s)	323
BisI	GCNGC	5 cut(s)	162, 183, 372, 875, 956
BlsI	GCNGC	5 cut(s)	163, 184, 373, 876, 957
Bme1390I	CCNGG	2 cut(s)	312, 1404
BmgT120I	GGNCC	1 cut(s)	1655
BmiI	GGNNCC	1 cut(s)	1453
BmrFI	CCNGG	2 cut(s)	312, 1404
BmrI	ACTGGG	1 cut(s)	1252
BmsI	GCATC	5 cut(s)	889, 919, 1304, 1480, 1614
BmuI	ACTGGG	1 cut(s)	1252
BpiI	GAAGAC	3 cut(s)	813, 1513, 1643
BpmI	CTGGAG	1 cut(s)	698
Bpu14I	TTCGAA	1 cut(s)	450
BpuEI	CTTGAG	2 cut(s)	437, 1202
BpuMI	CCSGG	1 cut(s)	1404
Bsa29I	ATCGAT	1 cut(s)	834
BsaI	GGTCTC	2 cut(s)	709, 1791
BsaJI	CCNNGG	1 cut(s)	1403
BsaWI	WCCGGW	1 cut(s)	218
BsaXI	ACNNNNNCTCC	2 cut(s)	558, 588
Bsc4I	CCNNNNNNNGG	2 cut(s)	1291, 1480
Bse118I	RCCGGY	1 cut(s)	539
Bse1I	ACTGG	4 cut(s)	681, 1247, 1420, 1630
Bse21I	CCTNAGG	1 cut(s)	613
Bse3DI	GCAATG	2 cut(s)	277, 345
BseAI	TCCGGA	1 cut(s)	218
BseBI	CCWGG	1 cut(s)	312
BseCI	ATCGAT	1 cut(s)	834
BseDI	CCNNGG	1 cut(s)	1403
BseGI	GGATG	3 cut(s)	584, 904, 1295
BseLI	CCNNNNNNNGG	2 cut(s)	1291, 1480
BseMI	GCAATG	2 cut(s)	277, 345
BseMII	CTCAG	5 cut(s)	102, 836, 882, 908, 974
BseNI	ACTGG	4 cut(s)	681, 1247, 1420, 1630
BseRI	GAGGAG	4 cut(s)	84, 128, 1002, 1199
BseXI	GCAGC	5 cut(s)	173, 194, 383, 861, 967
Bsh1236I	CGCG	2 cut(s)	915, 1613
Bsh1285I	CGRYCG	1 cut(s)	754
BshFI	GGCC	5 cut(s)	276, 963, 1284, 1419, 1656
BshVI	ATCGAT	1 cut(s)	834
BsiEI	CGRYCG	1 cut(s)	754
BsiHKAI	GWGCWC	1 cut(s)	1191
BsiSI	CCGG	3 cut(s)	219, 540, 1403
BslFI	GGGAC	1 cut(s)	583
BslI	CCNNNNNNNGG	2 cut(s)	1291, 1480
BsmAI	GTCTC	7 cut(s)	82, 515, 709, 785, 984, 1670, 1791
BsmFI	GGGAC	1 cut(s)	583
BsmI	GAATGC	2 cut(s)	501, 826
BsnI	GGCC	5 cut(s)	276, 963, 1284, 1419, 1656
Bso31I	GGTCTC	2 cut(s)	709, 1791
Bsp119I	TTCGAA	1 cut(s)	450
Bsp1286I	GDGCHC	3 cut(s)	240, 1191, 1702
Bsp13I	TCCGGA	1 cut(s)	218
Bsp143I	GATC	7 cut(s)	460, 579, 751, 1072, 1364, 1572, 1770
BspACI	CCGC	3 cut(s)	256, 268, 350
BspANI	GGCC	5 cut(s)	276, 963, 1284, 1419, 1656
BspCNI	CTCAG	5 cut(s)	103, 837, 881, 907, 975
BspDI	ATCGAT	1 cut(s)	834
BspEI	TCCGGA	1 cut(s)	218
BspFNI	CGCG	2 cut(s)	915, 1613
BspHI	TCATGA	1 cut(s)	1458
BspLI	GGNNCC	1 cut(s)	1453
BspMAI	CTGCAG	1 cut(s)	957
BspPI	GGATC	2 cut(s)	587, 1372
BspT104I	TTCGAA	1 cut(s)	450
BspTI	CTTAAG	1 cut(s)	323
BspTNI	GGTCTC	2 cut(s)	709, 1791
BsrDI	GCAATG	2 cut(s)	277, 345
BsrFI	RCCGGY	1 cut(s)	539
BsrI	ACTGG	4 cut(s)	681, 1247, 1420, 1630
BssAI	RCCGGY	1 cut(s)	539
BssECI	CCNNGG	1 cut(s)	1403
BssMI	GATC	7 cut(s)	460, 579, 751, 1072, 1364, 1572, 1770
Bst2UI	CCWGG	1 cut(s)	312
Bst4CI	ACNGT	6 cut(s)	378, 574, 663, 829, 1429, 1592
Bst6I	CTCTTC	4 cut(s)	243, 1306, 1317, 1668
BstAFI	CTTAAG	1 cut(s)	323
BstAPI	GCANNNNNTGC	1 cut(s)	380
BstBI	TTCGAA	1 cut(s)	450
BstC8I	GCNNGC	3 cut(s)	537, 1266, 1603
BstDEI	CTNAG	7 cut(s)	111, 613, 845, 868, 894, 983, 1256
BstENI	CCTNNNNNAGG	1 cut(s)	1478
BstF5I	GGATG	3 cut(s)	584, 904, 1295
BstFNI	CGCG	2 cut(s)	915, 1613
BstH2I	RGCGCY	2 cut(s)	216, 875
BstHHI	GCGC	2 cut(s)	215, 874
BstKTI	GATC	7 cut(s)	463, 582, 754, 1075, 1367, 1575, 1773
BstMAI	GTCTC	7 cut(s)	82, 515, 709, 785, 984, 1670, 1791
BstMBI	GATC	7 cut(s)	460, 579, 751, 1072, 1364, 1572, 1770
BstMCI	CGRYCG	1 cut(s)	754
BstMWI	GCNNNNNNNGC	3 cut(s)	158, 380, 1186
BstNI	CCWGG	1 cut(s)	312
BstNSI	RCATGY	1 cut(s)	1605
BstSCI	CCNGG	2 cut(s)	310, 1402
BstSFI	CTRYAG	2 cut(s)	953, 1693
BstUI	CGCG	2 cut(s)	915, 1613
BstV1I	GCAGC	5 cut(s)	173, 194, 383, 861, 967
BstV2I	GAAGAC	3 cut(s)	813, 1513, 1643
Bsu15I	ATCGAT	1 cut(s)	834
Bsu36I	CCTNAGG	1 cut(s)	613
BsuRI	GGCC	5 cut(s)	276, 963, 1284, 1419, 1656
BsuTUI	ATCGAT	1 cut(s)	834
BtgZI	GCGATG	2 cut(s)	896, 1827
BtsCI	GGATG	3 cut(s)	584, 904, 1295
BtsIMutI	CAGTG	4 cut(s)	108, 1127, 1425, 1568
Cac8I	GCNNGC	3 cut(s)	537, 1266, 1603
CciI	TCATGA	1 cut(s)	1458
CfoI	GCGC	2 cut(s)	215, 874
Cfr10I	RCCGGY	1 cut(s)	539
Cfr13I	GGNCC	1 cut(s)	1655
ClaI	ATCGAT	1 cut(s)	834
CseI	GACGC	1 cut(s)	444
CviAII	CATG	7 cut(s)	55, 403, 433, 1148, 1459, 1602, 1680
DdeI	CTNAG	7 cut(s)	111, 613, 845, 868, 894, 983, 1256
DpnI	GATC	7 cut(s)	462, 581, 753, 1074, 1366, 1574, 1772
DpnII	GATC	7 cut(s)	460, 579, 751, 1072, 1364, 1572, 1770
DrdI	GACNNNNNNGTC	1 cut(s)	1805
DseDI	GACNNNNNNGTC	1 cut(s)	1805
EaeI	YGGCCR	1 cut(s)	1417
Eam1104I	CTCTTC	4 cut(s)	243, 1306, 1317, 1668
EarI	CTCTTC	4 cut(s)	243, 1306, 1317, 1668
EciI	GGCGGA	1 cut(s)	245
Ecl136II	GAGCTC	1 cut(s)	1189
Eco147I	AGGCCT	1 cut(s)	1284
Eco24I	GRGCYC	3 cut(s)	240, 1191, 1702
Eco31I	GGTCTC	2 cut(s)	709, 1791
Eco32I	GATATC	1 cut(s)	1832
Eco47III	AGCGCT	1 cut(s)	873
Eco53kI	GAGCTC	1 cut(s)	1189
Eco57I	CTGAAG	4 cut(s)	86, 174, 264, 1507
Eco81I	CCTNAGG	1 cut(s)	613
EcoICRI	GAGCTC	1 cut(s)	1189
EcoNI	CCTNNNNNAGG	1 cut(s)	1478
EcoRII	CCWGG	1 cut(s)	310
EcoRV	GATATC	1 cut(s)	1832
EcoT22I	ATGCAT	2 cut(s)	1603, 1607
EcoT38I	GRGCYC	3 cut(s)	240, 1191, 1702
FaeI	CATG	7 cut(s)	58, 406, 436, 1151, 1462, 1605, 1683
FalI	AAGNNNNNCTT	4 cut(s)	768, 800, 1819, 1851
FaqI	GGGAC	1 cut(s)	583
FatI	CATG	7 cut(s)	54, 402, 432, 1147, 1458, 1601, 1679
FbaI	TGATCA	1 cut(s)	1572
FblI	GTMKAC	1 cut(s)	495
Fnu4HI	GCNGC	5 cut(s)	162, 183, 372, 875, 956
FokI	GGATG	3 cut(s)	571, 911, 1282
FriOI	GRGCYC	3 cut(s)	240, 1191, 1702
Fsp4HI	GCNGC	5 cut(s)	162, 183, 372, 875, 956
FspBI	CTAG	3 cut(s)	45, 696, 1043
GlaI	GCGC	2 cut(s)	214, 873
GluI	GCNGC	5 cut(s)	162, 183, 372, 875, 956
GsuI	CTGGAG	1 cut(s)	698
HaeII	RGCGCY	2 cut(s)	216, 875
HaeIII	GGCC	5 cut(s)	276, 963, 1284, 1419, 1656
HapII	CCGG	3 cut(s)	219, 540, 1403
HgaI	GACGC	1 cut(s)	444
HhaI	GCGC	2 cut(s)	215, 874
Hin1II	CATG	7 cut(s)	58, 406, 436, 1151, 1462, 1605, 1683
Hin6I	GCGC	2 cut(s)	213, 872
HinP1I	GCGC	2 cut(s)	213, 872
HincII	GTYRAC	1 cut(s)	360
HindII	GTYRAC	1 cut(s)	360
HindIII	AAGCTT	1 cut(s)	777
HinfI	GANTC	7 cut(s)	635, 647, 653, 716, 1165, 1374, 1386
HpaII	CCGG	3 cut(s)	219, 540, 1403
HphI	GGTGA	2 cut(s)	33, 1568
Hpy166II	GTNNAC	4 cut(s)	360, 496, 1425, 1690
Hpy188I	TCNGA	8 cut(s)	244, 460, 652, 1015, 1391, 1442, 1526, 1767
Hpy188III	TCNNGA	8 cut(s)	219, 442, 464, 720, 788, 896, 1459, 1706
Hpy8I	GTNNAC	4 cut(s)	360, 496, 1425, 1690
Hpy99I	CGWCG	1 cut(s)	484
HpyAV	CCTTC	8 cut(s)	61, 598, 794, 1254, 1491, 1712, 1720, 1851
HpyCH4III	ACNGT	6 cut(s)	378, 574, 663, 829, 1429, 1592
HpyCH4IV	ACGT	1 cut(s)	291
HpyF10VI	GCNNNNNNNGC	3 cut(s)	158, 380, 1186
HpyF3I	CTNAG	7 cut(s)	111, 613, 845, 868, 894, 983, 1256
HpySE526I	ACGT	1 cut(s)	291
Hsp92II	CATG	7 cut(s)	58, 406, 436, 1151, 1462, 1605, 1683
HspAI	GCGC	2 cut(s)	213, 872
Kpn2I	TCCGGA	1 cut(s)	218
Ksp22I	TGATCA	1 cut(s)	1572
Kzo9I	GATC	7 cut(s)	460, 579, 751, 1072, 1364, 1572, 1770
LmnI	GCTCC	2 cut(s)	97, 1186
Lsp1109I	GCAGC	5 cut(s)	173, 194, 383, 861, 967
LweI	GCATC	5 cut(s)	889, 919, 1304, 1480, 1614
MaeI	CTAG	3 cut(s)	45, 696, 1043
MaeII	ACGT	1 cut(s)	291
MaeIII	GTNAC	4 cut(s)	600, 1534, 1556, 1706
MalI	GATC	7 cut(s)	462, 581, 753, 1074, 1366, 1574, 1772
MboI	GATC	7 cut(s)	460, 579, 751, 1072, 1364, 1572, 1770
MfeI	CAATTG	1 cut(s)	1409
MhlI	GDGCHC	3 cut(s)	240, 1191, 1702
MlyI	GAGTC	5 cut(s)	644, 656, 662, 1159, 1383
MmeI	TCCRAC	4 cut(s)	105, 630, 1104, 1228
Mph1103I	ATGCAT	2 cut(s)	1603, 1607
MroI	TCCGGA	1 cut(s)	218
MroXI	GAANNNNTTC	2 cut(s)	226, 1057
MseI	TTAA	6 cut(s)	21, 324, 426, 1476, 1512, 1664
MspCI	CTTAAG	1 cut(s)	323
MspI	CCGG	3 cut(s)	219, 540, 1403
MspR9I	CCNGG	2 cut(s)	312, 1404
MunI	CAATTG	1 cut(s)	1409
Mva1269I	GAATGC	2 cut(s)	501, 826
MvaI	CCWGG	1 cut(s)	312
MvnI	CGCG	2 cut(s)	915, 1613
MwoI	GCNNNNNNNGC	3 cut(s)	158, 380, 1186
NciI	CCSGG	1 cut(s)	1404
NdeII	GATC	7 cut(s)	460, 579, 751, 1072, 1364, 1572, 1770
NlaIII	CATG	7 cut(s)	58, 406, 436, 1151, 1462, 1605, 1683
NlaIV	GGNNCC	1 cut(s)	1453
NmuCI	GTSAC	4 cut(s)	600, 1534, 1556, 1706
NsiI	ATGCAT	2 cut(s)	1603, 1607
NspI	RCATGY	1 cut(s)	1605
NspV	TTCGAA	1 cut(s)	450
PaeI	GCATGC	1 cut(s)	1605
PagI	TCATGA	1 cut(s)	1458
PceI	AGGCCT	1 cut(s)	1284
PctI	GAATGC	2 cut(s)	501, 826
PdmI	GAANNNNTTC	2 cut(s)	226, 1057
PfeI	GAWTC	2 cut(s)	716, 1386
PkrI	GCNGC	5 cut(s)	163, 184, 373, 876, 957
Ple19I	CGATCG	1 cut(s)	754
PleI	GAGTC	5 cut(s)	643, 655, 661, 1159, 1382
PpsI	GAGTC	5 cut(s)	643, 655, 661, 1159, 1382
PsiI	TTATAA	1 cut(s)	1596
Psp124BI	GAGCTC	1 cut(s)	1191
Psp6I	CCWGG	1 cut(s)	310
PspGI	CCWGG	1 cut(s)	310
PspN4I	GGNNCC	1 cut(s)	1453
PspPI	GGNCC	1 cut(s)	1655
PstI	CTGCAG	1 cut(s)	957
PvuI	CGATCG	1 cut(s)	754
SacI	GAGCTC	1 cut(s)	1191
SaqAI	TTAA	6 cut(s)	21, 324, 426, 1476, 1512, 1664
SatI	GCNGC	5 cut(s)	162, 183, 372, 875, 956
Sau3AI	GATC	7 cut(s)	460, 579, 751, 1072, 1364, 1572, 1770
Sau96I	GGNCC	1 cut(s)	1655
SchI	GAGTC	5 cut(s)	644, 656, 662, 1159, 1383
ScrFI	CCNGG	2 cut(s)	312, 1404
SduI	GDGCHC	3 cut(s)	240, 1191, 1702
SfaNI	GCATC	5 cut(s)	889, 919, 1304, 1480, 1614
SfcI	CTRYAG	2 cut(s)	953, 1693
SfuI	TTCGAA	1 cut(s)	450
SmlI	CTYRAG	3 cut(s)	323, 416, 1181
SmoI	CTYRAG	3 cut(s)	323, 416, 1181
SphI	GCATGC	1 cut(s)	1605
SseBI	AGGCCT	1 cut(s)	1284
SsiI	CCGC	3 cut(s)	256, 268, 350
SspMI	CTAG	3 cut(s)	45, 696, 1043
SstI	GAGCTC	1 cut(s)	1191
StuI	AGGCCT	1 cut(s)	1284
StyD4I	CCNGG	2 cut(s)	310, 1402
TaaI	ACNGT	6 cut(s)	378, 574, 663, 829, 1429, 1592
TaiI	ACGT	1 cut(s)	294
TfiI	GAWTC	2 cut(s)	716, 1386
Tru1I	TTAA	6 cut(s)	21, 324, 426, 1476, 1512, 1664
Tru9I	TTAA	6 cut(s)	21, 324, 426, 1476, 1512, 1664
TscAI	CASTG	4 cut(s)	115, 1127, 1432, 1575
TseFI	GTSAC	4 cut(s)	600, 1534, 1556, 1706
TseI	GCWGC	5 cut(s)	161, 182, 371, 874, 955
Tsp45I	GTSAC	4 cut(s)	600, 1534, 1556, 1706
TspDTI	ATGAA	8 cut(s)	102, 109, 147, 449, 677, 1164, 1223, 1767
TspGWI	ACGGA	1 cut(s)	377
TspRI	CASTG	4 cut(s)	115, 1127, 1432, 1575
Vha464I	CTTAAG	1 cut(s)	323
XagI	CCTNNNNNAGG	1 cut(s)	1478
XapI	RAATTY	4 cut(s)	409, 946, 1016, 1230
XceI	RCATGY	1 cut(s)	1605
XmiI	GTMKAC	1 cut(s)	495
XmnI	GAANNNNTTC	2 cut(s)	226, 1057
XspI	CTAG	3 cut(s)	45, 696, 1043
Zsp2I	ATGCAT	2 cut(s)	1603, 1607

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.