RchiOBHm_Chr5g0075421
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0006701
Physical Location & Seq
Forward (+)
81241248 .. 81243695
2448 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35014

Sequence Viewer

Length: 1530 bp
ATGAAAGTTGGTCATAGAAATCGTGCTGTTGGTGCTACAGCTCTGAATGAAAGAAGCAGTCGGTCTCATAGTGTCTTAACAGTCCATATACTTGGAAAGGAGTTGGCTTCTGGTTCTATTCTACGGGGTTGCCTCCATCTCGTGGATTTGGCAGGGAGCGAGAGGGTTGATAAATCAGAAGCCACTGGTGAGAGACTAAAGGAAGCTCAACATATAAATAGATCACTTTCTGCACTTGGAGATGTCATCTCTGCTCTTGCACAGAAGAGCGCACATGTTCCTTACAGAAACAGCAAACTTACTCAAGTCTTGCAAGATTCTTTAGGTGGTCAAGCCAAAACTATGATGTTTGTACATATAAACCCGGAACTTAATGCTCTTGGGGAGACGATTAGTACACTCAAGTTTGCTGAAAGGGTTGCATCCATAGAACTTGGGGCAGCTAAATCTAATAAAGAAACGGGAGAAATCCGGGAACTCAAAGAAGAGATATCAGATCTTAAACTAGCATTGGAAAAAAAGGAAGCTGAACTAAACCAAATCAAAGGTGGTACAAGAAACACCATTGATTCCCAAAAGTCTAGAGCCGTTTCACCTTTTCGTATTCCAAGGGGCATTGGCAACAGTTCAAAGCAAGAAACTTGTCAGCGACCCTTAGATGATGCTAAGATTTCTGAGGCAAGAAGCTGTTCTTCGGGCAAGCAAAGGAGATCAAGGTTTCCTTCTGCATTCACCGAGAAAGAGATCATTCCAAAGATACCATTTCCAGCTGAAGAAAGATTAGTGATCTCTGGAAAACACAGATCACCATCTCCTCCTGTCAGGCGATCAATATCTACTGATAGAGGGGCTCTCATTAGACCCAGAGTTAAGGCTGACACAACAGAAAACCAACCAATTGCGAAACCAACATTTCCATCAGCTAGAGTACCGGTCAATAAATCTCTTGCTAGTACCATGCCATCAATCCCATCAACGGAAAACTTGAGGGTACTTATCAGTTCACAAGAATCAACAAAGCACGATGACATTTCAGATGCATTGTATAGCTTCCAGAAGGCCAAAAGAGTTCATCCAGAAAATGAAGACGAGCAGTTTAAGCAAGCTCTTAATGTAAGACAAGGCGGTATCAGAAAAAGCAAGAATGAGACCAAGGCTAAGGCCAAGCAGAACCGAATACCAAAGTATGATGCAGTGACAGCTTTGTCTTCTGACTTGAATGGTCTTGAAAGGATCGAAGAGGCTCGAAAGAGTGACTTCTCGGAGCCAGAAAATGAGCATCTACCCATTAGTTCACCCATGCATAGTTCTTTGATGGCAAAAAAGCTTCGGCAAAACTCATCAAGGAACTATATAAATCTTGAACCACGAGGATCTGTGCAAGCAGCAGAACCTTTAATGGCTGGGAAAACAGAAAACAAGCTTCCAAATGGTGGAACTCGATATCAGAAAGAAGGCAGCAATATGTCCATGCCTGAATTAAGAAGAAGCCGGTCTACACCTCGCGGGAAATTTTTTAATTTTACATAG

Protein Analysis

509

Amino Acids

56.24

Weight (kDa)

9.94

Isoelectric Point (pI)

57.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinesin PF00225 1 - 143 1.3e-56 Kinesin motor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1204
AccB7I CCANNNNNTGG 2 cut(s) 142, 1433
AccI GTMKAC 1 cut(s) 1496
AccII CGCG 1 cut(s) 1506
AciI CCGC 2 cut(s) 1125, 1506
AclWI GGATC 2 cut(s) 1241, 1381
AcsI RAATTY 1 cut(s) 1511
AcuI CTGAAG 1 cut(s) 792
AfaI GTAC 6 cut(s) 354, 397, 553, 930, 955, 993
AfiI CCNNNNNNNGG 3 cut(s) 142, 976, 1433
AflIII ACRYGT 1 cut(s) 274
AgeI ACCGGT 1 cut(s) 931
AgsI TTSAA 4 cut(s) 630, 1219, 1229, 1364
Alw26I GTCTC 4 cut(s) 69, 187, 380, 1142
AlwI GGATC 2 cut(s) 1241, 1381
AoxI GGCC 2 cut(s) 1059, 1161
ApeKI GCWGC 3 cut(s) 440, 1385, 1458
ApoI RAATTY 1 cut(s) 1511
AsiGI ACCGGT 1 cut(s) 931
Asp700I GAANNNNTTC 1 cut(s) 688
AspLEI GCGC 1 cut(s) 272
AsuC2I CCSGG 2 cut(s) 365, 473
AsuHPI GGTGA 5 cut(s) 200, 585, 724, 798, 1287
BanII GRGCYC 1 cut(s) 853
BarI GAAGNNNNNNTAC 2 cut(s) 1480, 1512
BauI CACGAG 2 cut(s) 140, 1368
BbsI GAAGAC 2 cut(s) 1092, 1200
BbvI GCAGC 3 cut(s) 452, 1397, 1470
BccI CCATC 6 cut(s) 144, 817, 925, 970, 979, 1309
BceAI ACGGC 1 cut(s) 572
BcnI CCSGG 2 cut(s) 365, 473
BcoDI GTCTC 4 cut(s) 69, 187, 380, 1142
BfaI CTAG 4 cut(s) 506, 582, 924, 951
BfmI CTRYAG 1 cut(s) 36
BglII AGATCT 1 cut(s) 496
BisI GCNGC 3 cut(s) 441, 1386, 1459
BlsI GCNGC 3 cut(s) 442, 1387, 1460
Bme1390I CCNGG 2 cut(s) 365, 473
BmiI GGNNCC 1 cut(s) 1266
BmrFI CCNGG 2 cut(s) 365, 473
BmsI GCATC 5 cut(s) 431, 652, 1027, 1180, 1288
BpiI GAAGAC 2 cut(s) 1092, 1200
BplI GAGNNNNNCTC 2 cut(s) 837, 869
Bpu10I CCTNAGC 1 cut(s) 1158
BpuEI CTTGAG 3 cut(s) 288, 386, 1006
BpuMI CCSGG 2 cut(s) 365, 473
BsaBI GATNNNNATC 2 cut(s) 808, 832
BsaI GGTCTC 2 cut(s) 69, 1142
BsaJI CCNNGG 2 cut(s) 608, 1152
BsaWI WCCGGW 1 cut(s) 931
Bsc4I CCNNNNNNNGG 3 cut(s) 142, 976, 1433
Bse118I RCCGGY 2 cut(s) 931, 1491
Bse1I ACTGG 1 cut(s) 190
Bse8I GATNNNNATC 2 cut(s) 808, 832
BseDI CCNNGG 2 cut(s) 608, 1152
BseGI GGATG 2 cut(s) 422, 1072
BseJI GATNNNNATC 2 cut(s) 808, 832
BseLI CCNNNNNNNGG 3 cut(s) 142, 976, 1433
BseMII CTCAG 1 cut(s) 666
BseNI ACTGG 1 cut(s) 190
BseRI GAGGAG 1 cut(s) 804
BseXI GCAGC 3 cut(s) 452, 1397, 1470
BseYI CCCAGC 1 cut(s) 1403
BsgI GTGCAG 1 cut(s) 216
Bsh1236I CGCG 1 cut(s) 1506
BshFI GGCC 2 cut(s) 1061, 1163
BshTI ACCGGT 1 cut(s) 931
BsiSI CCGG 4 cut(s) 365, 472, 932, 1492
BslI CCNNNNNNNGG 3 cut(s) 142, 976, 1433
BsmAI GTCTC 4 cut(s) 69, 187, 380, 1142
BsmBI CGTCTC 1 cut(s) 380
BsmI GAATGC 1 cut(s) 728
BsnI GGCC 2 cut(s) 1061, 1163
Bso31I GGTCTC 2 cut(s) 69, 1142
Bsp1286I GDGCHC 1 cut(s) 853
Bsp1407I TGTACA 1 cut(s) 352
Bsp143I GATC 9 cut(s) 221, 496, 710, 744, 786, 803, 827, 1233, 1373
BspACI CCGC 2 cut(s) 1125, 1506
BspANI GGCC 2 cut(s) 1061, 1163
BspCNI CTCAG 1 cut(s) 667
BspFNI CGCG 1 cut(s) 1506
BspLI GGNNCC 1 cut(s) 1266
BspPI GGATC 2 cut(s) 1241, 1381
BspQI GCTCTTC 1 cut(s) 260
BspTNI GGTCTC 2 cut(s) 69, 1142
BsrFI RCCGGY 2 cut(s) 931, 1491
BsrGI TGTACA 1 cut(s) 352
BsrI ACTGG 1 cut(s) 190
BssAI RCCGGY 2 cut(s) 931, 1491
BssECI CCNNGG 2 cut(s) 608, 1152
BssMI GATC 9 cut(s) 221, 496, 710, 744, 786, 803, 827, 1233, 1373
BssSI CACGAG 2 cut(s) 140, 1368
BssT1I CCWWGG 2 cut(s) 608, 1152
Bst2BI CACGAG 2 cut(s) 140, 1368
Bst4CI ACNGT 2 cut(s) 82, 626
Bst6I CTCTTC 3 cut(s) 260, 480, 1233
BstAUI TGTACA 1 cut(s) 352
BstC8I GCNNGC 3 cut(s) 701, 1104, 1383
BstDEI CTNAG 4 cut(s) 655, 666, 675, 1158
BstF5I GGATG 2 cut(s) 422, 1072
BstFNI CGCG 1 cut(s) 1506
BstHHI GCGC 1 cut(s) 272
BstKTI GATC 9 cut(s) 224, 499, 713, 747, 789, 806, 830, 1236, 1376
BstMAI GTCTC 4 cut(s) 69, 187, 380, 1142
BstMBI GATC 9 cut(s) 221, 496, 710, 744, 786, 803, 827, 1233, 1373
BstMWI GCNNNNNNNGC 3 cut(s) 32, 1099, 1199
BstNSI RCATGY 1 cut(s) 278
BstSCI CCNGG 2 cut(s) 363, 471
BstSFI CTRYAG 1 cut(s) 36
BstUI CGCG 1 cut(s) 1506
BstV1I GCAGC 3 cut(s) 452, 1397, 1470
BstV2I GAAGAC 2 cut(s) 1092, 1200
BstX2I RGATCY 2 cut(s) 496, 1373
BstXI CCANNNNNNTGG 1 cut(s) 92
BstYI RGATCY 2 cut(s) 496, 1373
BsuRI GGCC 2 cut(s) 1061, 1163
BtsCI GGATG 2 cut(s) 422, 1072
BtsI GCAGTG 1 cut(s) 1200
BtsIMutI CAGTG 2 cut(s) 183, 1200
Cac8I GCNNGC 3 cut(s) 701, 1104, 1383
CfoI GCGC 1 cut(s) 272
Cfr10I RCCGGY 2 cut(s) 931, 1491
Csp6I GTAC 6 cut(s) 353, 396, 552, 929, 954, 992
CspAI ACCGGT 1 cut(s) 931
CviAII CATG 4 cut(s) 275, 958, 1300, 1471
CviQI GTAC 6 cut(s) 353, 396, 552, 929, 954, 992
DdeI CTNAG 4 cut(s) 655, 666, 675, 1158
DpnI GATC 9 cut(s) 223, 498, 712, 746, 788, 805, 829, 1235, 1375
DpnII GATC 9 cut(s) 221, 496, 710, 744, 786, 803, 827, 1233, 1373
DrdI GACNNNNNNGTC 1 cut(s) 1204
DseDI GACNNNNNNGTC 1 cut(s) 1204
Eam1104I CTCTTC 3 cut(s) 260, 480, 1233
EarI CTCTTC 3 cut(s) 260, 480, 1233
Eco130I CCWWGG 2 cut(s) 608, 1152
Eco24I GRGCYC 1 cut(s) 853
Eco31I GGTCTC 2 cut(s) 69, 1142
Eco32I GATATC 2 cut(s) 492, 1445
Eco57I CTGAAG 1 cut(s) 792
EcoRV GATATC 2 cut(s) 492, 1445
EcoT14I CCWWGG 2 cut(s) 608, 1152
EcoT22I ATGCAT 2 cut(s) 1042, 1305
EcoT38I GRGCYC 1 cut(s) 853
ErhI CCWWGG 2 cut(s) 608, 1152
Esp3I CGTCTC 1 cut(s) 380
FaeI CATG 4 cut(s) 278, 961, 1303, 1474
FalI AAGNNNNNCTT 6 cut(s) 676, 708, 706, 738, 1241, 1273
FatI CATG 4 cut(s) 274, 957, 1299, 1470
FauI CCCGC 1 cut(s) 1499
FblI GTMKAC 1 cut(s) 1496
Fnu4HI GCNGC 3 cut(s) 441, 1386, 1459
FokI GGATG 2 cut(s) 409, 1059
FriOI GRGCYC 1 cut(s) 853
Fsp4HI GCNGC 3 cut(s) 441, 1386, 1459
FspBI CTAG 4 cut(s) 506, 582, 924, 951
GlaI GCGC 1 cut(s) 271
GluI GCNGC 3 cut(s) 441, 1386, 1459
GsaI CCCAGC 1 cut(s) 1407
HaeIII GGCC 2 cut(s) 1061, 1163
HapII CCGG 4 cut(s) 365, 472, 932, 1492
HhaI GCGC 1 cut(s) 272
Hin1II CATG 4 cut(s) 278, 961, 1303, 1474
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HindIII AAGCTT 2 cut(s) 1325, 1421
HinfI GANTC 3 cut(s) 317, 569, 1010
HpaII CCGG 4 cut(s) 365, 472, 932, 1492
HphI GGTGA 5 cut(s) 200, 585, 724, 798, 1287
Hpy166II GTNNAC 4 cut(s) 398, 1004, 1295, 1497
Hpy188I TCNGA 9 cut(s) 45, 178, 496, 676, 1036, 1133, 1213, 1264, 1449
Hpy188III TCNNGA 6 cut(s) 582, 792, 1054, 1076, 1226, 1361
Hpy8I GTNNAC 4 cut(s) 398, 1004, 1295, 1497
HpyAV CCTTC 3 cut(s) 732, 1051, 1448
HpyCH4III ACNGT 2 cut(s) 82, 626
HpyCH4V TGCA 9 cut(s) 233, 260, 313, 422, 728, 1040, 1193, 1303, 1381
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 1099, 1199
HpyF3I CTNAG 4 cut(s) 655, 666, 675, 1158
Hsp92II CATG 4 cut(s) 278, 961, 1303, 1474
HspAI GCGC 1 cut(s) 270
Kzo9I GATC 9 cut(s) 221, 496, 710, 744, 786, 803, 827, 1233, 1373
LguI GCTCTTC 1 cut(s) 260
LmnI GCTCC 2 cut(s) 156, 1264
Lsp1109I GCAGC 3 cut(s) 452, 1397, 1470
LweI GCATC 5 cut(s) 431, 652, 1027, 1180, 1288
MaeI CTAG 4 cut(s) 506, 582, 924, 951
MaeIII GTNAC 2 cut(s) 1195, 1253
MalI GATC 9 cut(s) 223, 498, 712, 746, 788, 805, 829, 1235, 1375
MboI GATC 9 cut(s) 221, 496, 710, 744, 786, 803, 827, 1233, 1373
MboII GAAGA 8 cut(s) 277, 497, 684, 785, 1097, 1200, 1250, 1497
MfeI CAATTG 1 cut(s) 897
MflI RGATCY 2 cut(s) 496, 1373
MhlI GDGCHC 1 cut(s) 853
MluCI AATT 4 cut(s) 897, 1478, 1511, 1519
MnlI CCTC 9 cut(s) 143, 156, 670, 825, 839, 981, 1234, 1364, 1512
Mph1103I ATGCAT 2 cut(s) 1042, 1305
MroXI GAANNNNTTC 1 cut(s) 688
MseI TTAA 9 cut(s) 77, 372, 501, 870, 1098, 1110, 1397, 1481, 1518
MspA1I CMGCKG 1 cut(s) 770
MspI CCGG 4 cut(s) 365, 472, 932, 1492
MspR9I CCNGG 2 cut(s) 365, 473
MunI CAATTG 1 cut(s) 897
Mva1269I GAATGC 1 cut(s) 728
MvnI CGCG 1 cut(s) 1506
MwoI GCNNNNNNNGC 3 cut(s) 32, 1099, 1199
NciI CCSGG 2 cut(s) 365, 473
NdeII GATC 9 cut(s) 221, 496, 710, 744, 786, 803, 827, 1233, 1373
NlaIII CATG 4 cut(s) 278, 961, 1303, 1474
NlaIV GGNNCC 1 cut(s) 1266
NmuCI GTSAC 2 cut(s) 1195, 1253
NsiI ATGCAT 2 cut(s) 1042, 1305
NspI RCATGY 1 cut(s) 278
PciI ACATGT 1 cut(s) 274
PciSI GCTCTTC 1 cut(s) 260
PctI GAATGC 1 cut(s) 728
PdmI GAANNNNTTC 1 cut(s) 688
PfeI GAWTC 3 cut(s) 317, 569, 1010
PflMI CCANNNNNTGG 2 cut(s) 142, 1433
PfoI TCCNGGA 1 cut(s) 471
PinAI ACCGGT 1 cut(s) 931
PkrI GCNGC 3 cut(s) 442, 1387, 1460
PscI ACATGT 1 cut(s) 274
PspFI CCCAGC 1 cut(s) 1403
PspN4I GGNNCC 1 cut(s) 1266
PsuI RGATCY 2 cut(s) 496, 1373
PvuII CAGCTG 1 cut(s) 770
RsaI GTAC 6 cut(s) 354, 397, 553, 930, 955, 993
RsaNI GTAC 6 cut(s) 353, 396, 552, 929, 954, 992
SapI GCTCTTC 1 cut(s) 260
SaqAI TTAA 9 cut(s) 77, 372, 501, 870, 1098, 1110, 1397, 1481, 1518
SatI GCNGC 3 cut(s) 441, 1386, 1459
Sau3AI GATC 9 cut(s) 221, 496, 710, 744, 786, 803, 827, 1233, 1373
ScrFI CCNGG 2 cut(s) 365, 473
SduI GDGCHC 1 cut(s) 853
SfaNI GCATC 5 cut(s) 431, 652, 1027, 1180, 1288
SfcI CTRYAG 1 cut(s) 36
SmlI CTYRAG 3 cut(s) 303, 401, 985
SmoI CTYRAG 3 cut(s) 303, 401, 985
Sse9I AATT 4 cut(s) 897, 1478, 1511, 1519
SsiI CCGC 2 cut(s) 1125, 1506
SspMI CTAG 4 cut(s) 506, 582, 924, 951
StyD4I CCNGG 2 cut(s) 363, 471
StyI CCWWGG 2 cut(s) 608, 1152
TaaI ACNGT 2 cut(s) 82, 626
TaqI TCGA 3 cut(s) 1236, 1246, 1441
TaqII GACCGA 1 cut(s) 51
TasI AATT 4 cut(s) 897, 1478, 1511, 1519
TatI WGTACW 2 cut(s) 352, 395
TfiI GAWTC 3 cut(s) 317, 569, 1010
Tru1I TTAA 9 cut(s) 77, 372, 501, 870, 1098, 1110, 1397, 1481, 1518
Tru9I TTAA 9 cut(s) 77, 372, 501, 870, 1098, 1110, 1397, 1481, 1518
TscAI CASTG 2 cut(s) 190, 1200
TseFI GTSAC 2 cut(s) 1195, 1253
TseI GCWGC 3 cut(s) 440, 1385, 1458
Tsp45I GTSAC 2 cut(s) 1195, 1253
TspDTI ATGAA 4 cut(s) 17, 63, 1061, 1098
TspGWI ACGGA 1 cut(s) 992
TspRI CASTG 2 cut(s) 190, 1200
Van91I CCANNNNNTGG 2 cut(s) 142, 1433
XapI RAATTY 1 cut(s) 1511
XbaI TCTAGA 1 cut(s) 581
XceI RCATGY 1 cut(s) 278
XcmI CCANNNNNNNNNTGG 1 cut(s) 545
XmiI GTMKAC 1 cut(s) 1496
XmnI GAANNNNTTC 1 cut(s) 688
XspI CTAG 4 cut(s) 506, 582, 924, 951
Zsp2I ATGCAT 2 cut(s) 1042, 1305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.