Rorug05G0439500
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004229
Physical Location & Seq
Forward (+)
61075308 .. 61086610
11303 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0439500.1

Sequence Viewer

Length: 3081 bp
ATGGAAGACGAACATTCCGAAACGCCAGCCTCACTCATTCCCCCACTCCCAAGAGACTCCCGCGGTTCCCTCGAAGTATTCAACCCCTCCTCCTCGACCCGACCCGCCAGCCCCCCACCCTTCCGACCTTCCCAACCCGCCTCCTGGAATACCTGGATCGACCCGCCACGTGACACCCAACCCGACCCGCCGCCACCCAACGCCCCCTCCAAGTCCGGCCGCGACGTCGACGGCACCGTCACCTCATGGATGGCCCTCAAGGACTTCCCGCCAACTCCACCGCCGCCGTCGTCCAAGCCTTCAAAACAGTCCCAGAAATCCCCGCCGCTCATCCAGCTTACCATCTCCGCCTCGGTCAACGACAAGTCAACGCCGGAGCCGAGCGACGCCGCGCAGAGAGCCGCCGAGTGGGGATTGGTTCTGAAGACGGACACGGAGACGGGGAAGCCGCAGGGAGTGACCACCCGGAACTCGGGCGGACCCGAAGACCCGAGCCACAAACCGGGTACCTCCAGAAGAAACTCCAACAACTCGGTCCGGAGCTCCGGGGAGTTGTCCGACGACGGAGGCGGCGGAGGAGGAGGAGGAAAAGGGTTTCCGAGAGCTTCGGAGGACTTGAAGAACGTGCTGTCGACGTTTCAGCAGACGTTCGTGGTGTCGGATGCTACCAAACCCGATTACCCGATCATGTACGCCAGCGCCGGGTTTTTCAAGATGACCGGGTACACCTCCAAGGAGGTCATCGGCAGGAACTGCCGGTTTTTGCAGGGAGCGGATACGGACCCGGACGACGTGGCGCAGCTGAGGGAGGCTTTGCAGAAGAACACGAGCTATTGTGGTCGGTTGTTGAATTATAAGAAAGATGGGACCCCTTTTTGGAATTTACTTACTATTGCTCCCATTAAGGACGAAACTGGGAAGGTCCTCAAATTCATTGGAATGCAAGTGGAGGTGAGCAAGCACACTGAGGGGTCCAAGGATAAGATGCTGCGCCCCAATGGATTGCCTGAATCCCTCATTCGATATGATGCTCGTCAGAAAGAGATGGCTACACATTCAGTAACTGAGCTTGTGCAGGCAGTGAGGAGACCGAGGTCACTTAGTGAATCGATAAATCGTCCCTTTAGAAAGTCCGGAGGTGGCGGTAGGGGAGAGGAGGTAATAGAAGCTCTTGCGAGGAGAAACTCCGAGAGTTTGCCTAGGAGAAACTCAGAGAATGTGGCACCACCTAGACGTAACTCACTTGGGGATGCTAAAATCTCAATGCAGTGTATCAATGAGGTTCCTGAAAAGAAACAGAAAAAATCTAGACGGCGCTCTTTCATGGGGGTTATTCGGAAAAGTCAAACTCAAAGCCAACCCGATGATGATACATTTGACAACTTTGGAGATTCTGAGGATGTCCGTGATGACAGTGACAATGATGATGAGAGACCTATGAGTCTTGAAGATAAAGCGAGGAAGAAGGAAATGAGGAAGGGTATAGATCTTGCTACGACGCTGGAACGTATAGAGAAAAACTTTGTCATTACTGATCCAAGGCTGCCTGATAATCCCATTATATTTGCATCAGACAGCTTCTTAGAGCTAACGGAGTACAGCCGCGAAGAAATATTGGGAAGAAATTGCAGATTCCTTCAAGGATCTGAGACTGATCCAGCAACTGTGAAAAAAATAAGAGACGCAATTGACAACCAGACTGACGTGACTGTACAACTCATCAATTACACGAAAAGCGGCAAAAAGTTCTGGAATGTCTTTCATCTGCAGCCTATGCGTGATCAAAAGGGAGAAGTACAGTATTTTATTGGAGTACAACTTGATGGCAGTCAGCACATTGAGCCTCTGCATAACTCTATTCCTGAAGTTACAGCAAAAGAGAGTGAAAAACTGGTCAAAGAGACTGCAGTTAATGTTGATGAGGCAGCAAGAGAACTTCCAGATGCCAATATGAAACCAGAAGATTTATGGATGAATCATTCGAAGATCGTTCATCCAAAGCCTCATAGAAGGGACAGCCCCCCTTGGAGAGCTATTCAAAAGATTCTAGACAGTGGAGAACAGATAGGATTGAAACATTTTAAGCCTATAAAGCCCTTGGGATCTGGTGATACTGGCAGTGTGCATTTGGTCCAATTGTGTGGAACTGATCAGTACTACGCCATGAAGGCTATGGATAAGGGTATCATGCTCAATAGGAACAAGGTGCACAGAGCTTGTGCAGAGAGAGAAATCCTCGACATGTTGGATCACCCTTTTCTTCCTGCATTGTATGCTTCGTTTCAGACCAAAACACATGTTTGCCTGATCACTGATTACTACCCTGGTGGAGAACTCTTCCTACTTTTGGACAGCCAACCAACGAAGATACTGAAGGAAGATTCAGTGAGATTTTATGTTGCAGAGGTAGTTGTTGCCTTGGAGTATCTTCACTGTCAAGGGATAATTTACAGGGATTTGAAGCCTGAAAATGTTTTACTTCAGAGCAGTGGGCACGTAACCCTGACCGATTTTGATTTATCGTGTTTGACATCTTGCAAACCACAGCTTTTAATTCCGACCATAAACGAAAAGAAAAGGCATCACAAAGGTCAACAGAATCCAATCTTCATGGCTGAACCAATGAGAGCATCAAATTCTTTTGTTGGAACGGAAGAGTACATCGCTCCGGAAATCATAACTGGAGCTGGCCATTCTAGTGCAGTAGACTGGTGGGCTCTTGGTATTCTTATATATGAAATGCTCTATGGATATACACCATTTAGGGGAAAGACAAGGCAAAAGACGTTCGCCAATATTCTTCACAAGGATCTCAAATTTCCAGGAAGTATACCGGCAAGTCTCCAAGCAAAACAGTTAATGTATCGGTTGTTGCATCGTGATCCCAAAAACAGATTGGGGTCTCAGGAAGGGGCAAGCGAAATTAAGCGTCACCCTTTCTTTCGGGGTATCAACTGGGCTCTAGTTCGCTGCATGAACCCTCCTCAACTCGATGCTCCTCTCTTCGGGACAACTGAAGCTGAAAAGGGTGCTAAGCTTGTGGATCCTGAGATGCAAGATCTACAAACAAATATTTTCTAA

Protein Analysis

1026

Amino Acids

114.73

Weight (kDa)

7.87

Isoelectric Point (pI)

49.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAS_9 PF13426 223 - 319 3.4e-20 PAS domain
PAS PF00989 228 - 314 7.4e-08 PAS fold
PAS_3 PF08447 229 - 313 2.5e-07 PAS fold
PAS_9 PF13426 516 - 608 2e-19 PAS domain
PAS PF00989 519 - 608 3.4e-06 PAS fold
PAS_3 PF08447 520 - 605 7.5e-06 PAS fold
Pkinase PF00069 696 - 981 9.3e-60 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 697 - 843 1.2e-16 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 886 - 966 5.4e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 855
AasI GACNNNNNNGTC 2 cut(s) 236, 1440
AatII GACGTC 1 cut(s) 228
Acc65I GGTACC 1 cut(s) 506
AccB1I GGYRCC 3 cut(s) 233, 506, 1222
AccBSI CCGCTC 2 cut(s) 328, 773
AccI GTMKAC 4 cut(s) 228, 632, 2706, 2830
AccII CGCG 4 cut(s) 63, 222, 392, 1605
AccIII TCCGGA 3 cut(s) 537, 1133, 2668
AcoI YGGCCR 2 cut(s) 217, 2689
AcsI RAATTY 4 cut(s) 880, 929, 2635, 2816
AcuI CTGAAG 5 cut(s) 443, 1884, 2393, 2465, 3036
AcvI CACGTG 1 cut(s) 170
AcyI GRCGYC 2 cut(s) 225, 387
AdeI CACNNNGTG 1 cut(s) 1103
AfaI GTAC 9 cut(s) 508, 692, 725, 1598, 1713, 1797, 1815, 2156, 2660
AfiI CCNNNNNNNGG 9 cut(s) 144, 408, 472, 502, 702, 876, 2347, 2765, 3005
AflIII ACRYGT 2 cut(s) 2241, 2296
AjiI CACGTC 2 cut(s) 793, 1705
AjnI CCWGG 4 cut(s) 143, 152, 2323, 2821
Alw21I GWGCWC 2 cut(s) 545, 2211
Alw26I GTCTC 9 cut(s) 48, 431, 1081, 1426, 1643, 1674, 1895, 2846, 2907
Alw44I GTGCAC 1 cut(s) 2207
AlwNI CAGNNNCTG 3 cut(s) 753, 1064, 1664
Ama87I CYCGRG 2 cut(s) 472, 490
Aor13HI TCCGGA 3 cut(s) 537, 1133, 2668
AoxI GGCC 3 cut(s) 217, 252, 2689
ApaLI GTGCAC 1 cut(s) 2207
ApeKI GCWGC 6 cut(s) 799, 988, 1543, 1768, 1925, 2970
ApoI RAATTY 4 cut(s) 880, 929, 2635, 2816
Asp718I GGTACC 1 cut(s) 506
AspA2I CCTAGG 1 cut(s) 1199
AspLEI GCGC 5 cut(s) 394, 701, 799, 993, 1317
AspS9I GGNCC 8 cut(s) 253, 479, 535, 781, 867, 922, 972, 2131
AsuC2I CCSGG 6 cut(s) 466, 504, 547, 703, 721, 785
AsuHPI GGTGA 5 cut(s) 232, 964, 2120, 2243, 2924
AsuII TTCGAA 1 cut(s) 1982
AvaI CYCGRG 2 cut(s) 472, 490
AvaII GGWCC 7 cut(s) 479, 535, 781, 867, 922, 972, 2131
AvrII CCTAGG 1 cut(s) 1199
BaeGI GKGCMC 2 cut(s) 2211, 2496
BalI TGGCCA 1 cut(s) 2691
BamHI GGATCC 1 cut(s) 3043
BanI GGYRCC 3 cut(s) 233, 506, 1222
BanII GRGCYC 3 cut(s) 545, 2719, 2962
BauI CACGAG 1 cut(s) 826
BbrPI CACGTG 1 cut(s) 170
BbsI GAAGAC 3 cut(s) 12, 431, 492
Bbv12I GWGCWC 2 cut(s) 545, 2211
BbvCI CCTCAGC 1 cut(s) 803
BbvI GCAGC 6 cut(s) 811, 975, 1530, 1780, 1937, 2957
BccI CCATC 5 cut(s) 244, 350, 857, 1039, 1817
BceAI ACGGC 3 cut(s) 247, 271, 1328
BciT130I CCWGG 4 cut(s) 145, 154, 2325, 2823
BciVI GTATCC 1 cut(s) 769
BclI TGATCA 3 cut(s) 1780, 2149, 2307
BcnI CCSGG 6 cut(s) 466, 504, 547, 703, 721, 785
BcoDI GTCTC 9 cut(s) 48, 431, 1081, 1426, 1643, 1674, 1895, 2846, 2907
BfaI CTAG 6 cut(s) 1200, 1230, 1308, 2048, 2697, 2963
BfmI CTRYAG 2 cut(s) 1766, 1905
BfoI RGCGCY 2 cut(s) 702, 1318
BfuI GTATCC 1 cut(s) 769
BglI GCCNNNNNGGC 1 cut(s) 2168
BglII AGATCT 2 cut(s) 1486, 3058
BlnI CCTAGG 1 cut(s) 1199
BlpI GCTNAGC 1 cut(s) 3033
BmcAI AGTACT 1 cut(s) 2156
Bme18I GGWCC 7 cut(s) 479, 535, 781, 867, 922, 972, 2131
BmeT110I CYCGRG 2 cut(s) 472, 490
BmgBI CACGTC 2 cut(s) 793, 1705
BmgT120I GGNCC 8 cut(s) 253, 479, 535, 781, 867, 922, 972, 2131
BmrI ACTGGG 2 cut(s) 924, 2965
BmuI ACTGGG 2 cut(s) 924, 2965
BoxI GACNNNNGTC 1 cut(s) 1093
BpiI GAAGAC 3 cut(s) 12, 431, 492
BplI GAGNNNNNCTC 2 cut(s) 2220, 2252
BpmI CTGGAG 2 cut(s) 496, 2703
Bpu10I CCTNAGC 1 cut(s) 803
Bpu1102I GCTNAGC 1 cut(s) 3033
Bpu14I TTCGAA 1 cut(s) 1982
BpuEI CTTGAG 1 cut(s) 242
BpuMI CCSGG 6 cut(s) 466, 504, 547, 703, 721, 785
Bsa29I ATCGAT 1 cut(s) 1109
BsaAI YACGTR 2 cut(s) 170, 2497
BsaHI GRCGYC 2 cut(s) 225, 387
BsaI GGTCTC 3 cut(s) 1081, 1426, 2907
BsaWI WCCGGW 3 cut(s) 537, 1133, 2668
BsaXI ACNNNNNCTCC 8 cut(s) 74, 104, 191, 221, 880, 910, 1143, 1173
Bsc4I CCNNNNNNNGG 9 cut(s) 144, 408, 472, 502, 702, 876, 2347, 2765, 3005
Bse118I RCCGGY 2 cut(s) 756, 2833
Bse1I ACTGG 6 cut(s) 919, 1896, 2119, 2686, 2714, 2960
BseAI TCCGGA 3 cut(s) 537, 1133, 2668
BseBI CCWGG 4 cut(s) 145, 154, 2325, 2823
BseCI ATCGAT 1 cut(s) 1109
BseGI GGATG 7 cut(s) 255, 330, 667, 1255, 1405, 1977, 1993
BseLI CCNNNNNNNGG 9 cut(s) 144, 408, 472, 502, 702, 876, 2347, 2765, 3005
BseMII CTCAG 8 cut(s) 794, 957, 1056, 1224, 1386, 1638, 2918, 3039
BseNI ACTGG 6 cut(s) 919, 1896, 2119, 2686, 2714, 2960
BseSI GKGCMC 2 cut(s) 2211, 2496
BseX3I CGGCCG 1 cut(s) 217
BseXI GCAGC 6 cut(s) 811, 975, 1530, 1780, 1937, 2957
BsgI GTGCAG 3 cut(s) 1094, 2241, 2721
Bsh1236I CGCG 4 cut(s) 63, 222, 392, 1605
Bsh1285I CGRYCG 1 cut(s) 220
BshFI GGCC 3 cut(s) 219, 254, 2691
BshNI GGYRCC 3 cut(s) 233, 506, 1222
BshVI ATCGAT 1 cut(s) 1109
BsiEI CGRYCG 1 cut(s) 220
BsiHKAI GWGCWC 2 cut(s) 545, 2211
BsiHKCI CYCGRG 2 cut(s) 472, 490
BslFI GGGAC 5 cut(s) 295, 880, 1104, 2027, 3022
BslI CCNNNNNNNGG 9 cut(s) 144, 408, 472, 502, 702, 876, 2347, 2765, 3005
BsmAI GTCTC 9 cut(s) 48, 431, 1081, 1426, 1643, 1674, 1895, 2846, 2907
BsmBI CGTCTC 2 cut(s) 431, 1674
BsmFI GGGAC 5 cut(s) 295, 880, 1104, 2027, 3022
BsmI GAATGC 1 cut(s) 945
BsnI GGCC 3 cut(s) 219, 254, 2691
Bso31I GGTCTC 3 cut(s) 1081, 1426, 2907
BsoBI CYCGRG 2 cut(s) 472, 490
Bsp119I TTCGAA 1 cut(s) 1982
Bsp1286I GDGCHC 5 cut(s) 545, 2211, 2496, 2719, 2962
Bsp13I TCCGGA 3 cut(s) 537, 1133, 2668
Bsp1407I TGTACA 1 cut(s) 1711
Bsp1720I GCTNAGC 1 cut(s) 3033
BspANI GGCC 3 cut(s) 219, 254, 2691
BspCNI CTCAG 8 cut(s) 795, 958, 1057, 1223, 1387, 1639, 2917, 3040
BspDI ATCGAT 1 cut(s) 1109
BspEI TCCGGA 3 cut(s) 537, 1133, 2668
BspFNI CGCG 4 cut(s) 63, 222, 392, 1605
BspMAI CTGCAG 2 cut(s) 1770, 1909
BspT104I TTCGAA 1 cut(s) 1982
BspT107I GGYRCC 3 cut(s) 233, 506, 1222
BspTNI GGTCTC 3 cut(s) 1081, 1426, 2907
BsrBI CCGCTC 2 cut(s) 328, 773
BsrFI RCCGGY 2 cut(s) 756, 2833
BsrGI TGTACA 1 cut(s) 1711
BsrI ACTGG 6 cut(s) 919, 1896, 2119, 2686, 2714, 2960
BssAI RCCGGY 2 cut(s) 756, 2833
BssNAI GTATAC 1 cut(s) 2831
BssNI GRCGYC 2 cut(s) 225, 387
BssSI CACGAG 1 cut(s) 826
BssT1I CCWWGG 7 cut(s) 732, 975, 1199, 1538, 2024, 2097, 2418
Bst1107I GTATAC 1 cut(s) 2831
Bst2BI CACGAG 1 cut(s) 826
Bst2UI CCWGG 4 cut(s) 145, 154, 2325, 2823
Bst4CI ACNGT 9 cut(s) 238, 309, 1415, 1666, 1711, 1800, 2054, 2435, 2856
Bst6I CTCTTC 3 cut(s) 2342, 2649, 3008
BstACI GRCGYC 2 cut(s) 225, 387
BstAPI GCANNNNNTGC 3 cut(s) 753, 1774, 2273
BstAUI TGTACA 1 cut(s) 1711
BstBAI YACGTR 2 cut(s) 170, 2497
BstBI TTCGAA 1 cut(s) 1982
BstC8I GCNNGC 7 cut(s) 27, 109, 697, 959, 1077, 2689, 2917
BstDSI CCRYGG 1 cut(s) 61
BstF5I GGATG 7 cut(s) 255, 330, 667, 1255, 1405, 1977, 1993
BstFNI CGCG 4 cut(s) 63, 222, 392, 1605
BstH2I RGCGCY 2 cut(s) 702, 1318
BstHHI GCGC 5 cut(s) 394, 701, 799, 993, 1317
BstMAI GTCTC 9 cut(s) 48, 431, 1081, 1426, 1643, 1674, 1895, 2846, 2907
BstMCI CGRYCG 1 cut(s) 220
BstMWI GCNNNNNNNGC 8 cut(s) 334, 398, 753, 1774, 1840, 2092, 2168, 2273
BstNI CCWGG 4 cut(s) 145, 154, 2325, 2823
BstNSI RCATGY 2 cut(s) 2245, 2300
BstPAI GACNNNNGTC 1 cut(s) 1093
BstSFI CTRYAG 2 cut(s) 1766, 1905
BstSLI GKGCMC 2 cut(s) 2211, 2496
BstUI CGCG 4 cut(s) 63, 222, 392, 1605
BstV1I GCAGC 6 cut(s) 811, 975, 1530, 1780, 1937, 2957
BstV2I GAAGAC 3 cut(s) 12, 431, 492
BstX2I RGATCY 6 cut(s) 1486, 1643, 2102, 2809, 3043, 3058
BstXI CCANNNNNNTGG 1 cut(s) 2141
BstYI RGATCY 6 cut(s) 1486, 1643, 2102, 2809, 3043, 3058
BstZ17I GTATAC 1 cut(s) 2831
BstZI CGGCCG 1 cut(s) 217
Bsu15I ATCGAT 1 cut(s) 1109
BsuI GTATCC 1 cut(s) 769
BsuRI GGCC 3 cut(s) 219, 254, 2691
BsuTUI ATCGAT 1 cut(s) 1109
BtgI CCRYGG 1 cut(s) 61
BtgZI GCGATG 1 cut(s) 2647
BtrI CACGTC 2 cut(s) 793, 1705
BtsCI GGATG 7 cut(s) 255, 330, 667, 1255, 1405, 1977, 1993
BtsI GCAGTG 4 cut(s) 1086, 1274, 2125, 2494
Cac8I GCNNGC 7 cut(s) 27, 109, 697, 959, 1077, 2689, 2917
CaiI CAGNNNCTG 3 cut(s) 753, 1064, 1664
CfoI GCGC 5 cut(s) 394, 701, 799, 993, 1317
Cfr10I RCCGGY 2 cut(s) 756, 2833
Cfr13I GGNCC 8 cut(s) 253, 479, 535, 781, 867, 922, 972, 2131
Cfr42I CCGCGG 1 cut(s) 64
ClaI ATCGAT 1 cut(s) 1109
CpoI CGGWCCG 1 cut(s) 535
CseI GACGC 4 cut(s) 395, 1507, 1691, 2918
Csp6I GTAC 9 cut(s) 507, 691, 724, 1597, 1712, 1796, 1814, 2155, 2659
CspI CGGWCCG 1 cut(s) 535
CviAII CATG 9 cut(s) 246, 688, 1324, 2164, 2188, 2242, 2297, 2611, 2974
CviQI GTAC 9 cut(s) 507, 691, 724, 1597, 1712, 1796, 1814, 2155, 2659
DraIII CACNNNGTG 1 cut(s) 1103
DrdI GACNNNNNNGTC 2 cut(s) 236, 1440
DseDI GACNNNNNNGTC 2 cut(s) 236, 1440
EaeI YGGCCR 2 cut(s) 217, 2689
EagI CGGCCG 1 cut(s) 217
Eam1104I CTCTTC 3 cut(s) 2342, 2649, 3008
EarI CTCTTC 3 cut(s) 2342, 2649, 3008
EciI GGCGGA 3 cut(s) 337, 492, 588
Ecl136II GAGCTC 1 cut(s) 543
EclXI CGGCCG 1 cut(s) 217
Eco130I CCWWGG 7 cut(s) 732, 975, 1199, 1538, 2024, 2097, 2418
Eco24I GRGCYC 3 cut(s) 545, 2719, 2962
Eco31I GGTCTC 3 cut(s) 1081, 1426, 2907
Eco47I GGWCC 7 cut(s) 479, 535, 781, 867, 922, 972, 2131
Eco52I CGGCCG 1 cut(s) 217
Eco53kI GAGCTC 1 cut(s) 543
Eco57I CTGAAG 5 cut(s) 443, 1884, 2393, 2465, 3036
Eco72I CACGTG 1 cut(s) 170
Eco88I CYCGRG 2 cut(s) 472, 490
EcoICRI GAGCTC 1 cut(s) 543
EcoO109I RGGNCCY 2 cut(s) 867, 922
EcoRII CCWGG 4 cut(s) 143, 152, 2323, 2821
EcoT14I CCWWGG 7 cut(s) 732, 975, 1199, 1538, 2024, 2097, 2418
EcoT38I GRGCYC 3 cut(s) 545, 2719, 2962
ErhI CCWWGG 7 cut(s) 732, 975, 1199, 1538, 2024, 2097, 2418
Esp3I CGTCTC 2 cut(s) 431, 1674
FaeI CATG 9 cut(s) 249, 691, 1327, 2167, 2191, 2245, 2300, 2614, 2977
FaqI GGGAC 5 cut(s) 295, 880, 1104, 2027, 3022
FatI CATG 9 cut(s) 245, 687, 1323, 2163, 2187, 2241, 2296, 2610, 2973
FauI CCCGC 7 cut(s) 68, 112, 145, 171, 195, 276, 330
FbaI TGATCA 3 cut(s) 1780, 2149, 2307
FblI GTMKAC 4 cut(s) 228, 632, 2706, 2830
FokI GGATG 7 cut(s) 262, 317, 674, 1262, 1412, 1980, 1984
FriOI GRGCYC 3 cut(s) 545, 2719, 2962
FspBI CTAG 6 cut(s) 1200, 1230, 1308, 2048, 2697, 2963
GlaI GCGC 5 cut(s) 393, 700, 798, 992, 1316
GsuI CTGGAG 2 cut(s) 496, 2703
HaeII RGCGCY 2 cut(s) 702, 1318
HaeIII GGCC 3 cut(s) 219, 254, 2691
HgaI GACGC 4 cut(s) 395, 1507, 1691, 2918
HhaI GCGC 5 cut(s) 394, 701, 799, 993, 1317
Hin1I GRCGYC 2 cut(s) 225, 387
Hin1II CATG 9 cut(s) 249, 691, 1327, 2167, 2191, 2245, 2300, 2614, 2977
Hin6I GCGC 5 cut(s) 392, 699, 797, 991, 1315
HinP1I GCGC 5 cut(s) 392, 699, 797, 991, 1315
HincII GTYRAC 5 cut(s) 229, 358, 369, 633, 2594
HindII GTYRAC 5 cut(s) 229, 358, 369, 633, 2594
HindIII AAGCTT 1 cut(s) 3035
HphI GGTGA 5 cut(s) 232, 964, 2120, 2243, 2924
Hpy166II GTNNAC 9 cut(s) 229, 358, 369, 633, 726, 2209, 2594, 2707, 2831
Hpy8I GTNNAC 9 cut(s) 229, 358, 369, 633, 726, 2209, 2594, 2707, 2831
HpyCH4III ACNGT 9 cut(s) 238, 309, 1415, 1666, 1711, 1800, 2054, 2435, 2856
HpyF10VI GCNNNNNNNGC 8 cut(s) 334, 398, 753, 1774, 1840, 2092, 2168, 2273
Hsp92I GRCGYC 2 cut(s) 225, 387
Hsp92II CATG 9 cut(s) 249, 691, 1327, 2167, 2191, 2245, 2300, 2614, 2977
HspAI GCGC 5 cut(s) 392, 699, 797, 991, 1315
KflI GGGWCCC 1 cut(s) 867
Kpn2I TCCGGA 3 cut(s) 537, 1133, 2668
KpnI GGTACC 1 cut(s) 510
Ksp22I TGATCA 3 cut(s) 1780, 2149, 2307
KspI CCGCGG 1 cut(s) 64
LmnI GCTCC 8 cut(s) 376, 540, 548, 770, 901, 2671, 2684, 3001
Lsp1109I GCAGC 6 cut(s) 811, 975, 1530, 1780, 1937, 2957
MaeI CTAG 6 cut(s) 1200, 1230, 1308, 2048, 2697, 2963
MbiI CCGCTC 2 cut(s) 328, 773
MfeI CAATTG 2 cut(s) 1686, 2135
MflI RGATCY 6 cut(s) 1486, 1643, 2102, 2809, 3043, 3058
MhlI GDGCHC 5 cut(s) 545, 2211, 2496, 2719, 2962
MlsI TGGCCA 1 cut(s) 2691
MluNI TGGCCA 1 cut(s) 2691
MlyI GAGTC 2 cut(s) 50, 1450
MmeI TCCRAC 7 cut(s) 148, 549, 582, 639, 2226, 2582, 2626
Mox20I TGGCCA 1 cut(s) 2691
MroI TCCGGA 3 cut(s) 537, 1133, 2668
MscI TGGCCA 1 cut(s) 2691
MseI TTAA 6 cut(s) 903, 1911, 2082, 2552, 2858, 2925
MslI CAYNNNNRTG 2 cut(s) 938, 2697
Msp20I TGGCCA 1 cut(s) 2691
MspA1I CMGCKG 2 cut(s) 63, 802
MunI CAATTG 2 cut(s) 1686, 2135
Mva1269I GAATGC 1 cut(s) 945
MvaI CCWGG 4 cut(s) 145, 154, 2325, 2823
MvnI CGCG 4 cut(s) 63, 222, 392, 1605
MwoI GCNNNNNNNGC 8 cut(s) 334, 398, 753, 1774, 1840, 2092, 2168, 2273
NciI CCSGG 6 cut(s) 466, 504, 547, 703, 721, 785
NlaIII CATG 9 cut(s) 249, 691, 1327, 2167, 2191, 2245, 2300, 2614, 2977
NmeAIII GCCGAG 2 cut(s) 405, 430
NmuCI GTSAC 7 cut(s) 170, 238, 457, 1095, 1415, 1705, 2930
NspI RCATGY 2 cut(s) 2245, 2300
NspV TTCGAA 1 cut(s) 1982
PciI ACATGT 2 cut(s) 2241, 2296
PcsI WCGNNNNNNNCGW 3 cut(s) 15, 234, 377
PctI GAATGC 1 cut(s) 945
PfeI GAWTC 8 cut(s) 1010, 1106, 1391, 1632, 1975, 2044, 2381, 2599
PfoI TCCNGGA 2 cut(s) 143, 2821
PleI GAGTC 2 cut(s) 50, 1449
PmaCI CACGTG 1 cut(s) 170
PmlI CACGTG 1 cut(s) 170
PpsI GAGTC 2 cut(s) 50, 1449
Ppu21I YACGTR 2 cut(s) 170, 2497
PpuMI RGGWCCY 2 cut(s) 867, 922
PscI ACATGT 2 cut(s) 2241, 2296
PshAI GACNNNNGTC 1 cut(s) 1093
PsiI TTATAA 1 cut(s) 855
Psp124BI GAGCTC 1 cut(s) 545
Psp5II RGGWCCY 2 cut(s) 867, 922
Psp6I CCWGG 4 cut(s) 143, 152, 2323, 2821
PspCI CACGTG 1 cut(s) 170
PspGI CCWGG 4 cut(s) 143, 152, 2323, 2821
PspPI GGNCC 8 cut(s) 253, 479, 535, 781, 867, 922, 972, 2131
PspPPI RGGWCCY 2 cut(s) 867, 922
PsrI GAACNNNNNNTAC 2 cut(s) 2325, 2357
PstI CTGCAG 2 cut(s) 1770, 1909
PstNI CAGNNNCTG 3 cut(s) 753, 1064, 1664
PsuI RGATCY 6 cut(s) 1486, 1643, 2102, 2809, 3043, 3058
PvuII CAGCTG 1 cut(s) 802
RsaI GTAC 9 cut(s) 508, 692, 725, 1598, 1713, 1797, 1815, 2156, 2660
RsaNI GTAC 9 cut(s) 507, 691, 724, 1597, 1712, 1796, 1814, 2155, 2659
RseI CAYNNNNRTG 2 cut(s) 938, 2697
Rsr2I CGGWCCG 1 cut(s) 535
RsrII CGGWCCG 1 cut(s) 535
SacI GAGCTC 1 cut(s) 545
SacII CCGCGG 1 cut(s) 64
SalI GTCGAC 2 cut(s) 227, 631
SaqAI TTAA 6 cut(s) 903, 1911, 2082, 2552, 2858, 2925
Sau96I GGNCC 8 cut(s) 253, 479, 535, 781, 867, 922, 972, 2131
ScaI AGTACT 1 cut(s) 2156
SchI GAGTC 2 cut(s) 50, 1450
SduI GDGCHC 5 cut(s) 545, 2211, 2496, 2719, 2962
SfcI CTRYAG 2 cut(s) 1766, 1905
Sfr303I CCGCGG 1 cut(s) 64
SfuI TTCGAA 1 cut(s) 1982
SgrBI CCGCGG 1 cut(s) 64
SgrDI CGTCGACG 1 cut(s) 227
SinI GGWCC 7 cut(s) 479, 535, 781, 867, 922, 972, 2131
SmiMI CAYNNNNRTG 2 cut(s) 938, 2697
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
SspI AATATT 3 cut(s) 1614, 2797, 3073
SspMI CTAG 6 cut(s) 1200, 1230, 1308, 2048, 2697, 2963
SstI GAGCTC 1 cut(s) 545
StyI CCWWGG 7 cut(s) 732, 975, 1199, 1538, 2024, 2097, 2418
TaaI ACNGT 9 cut(s) 238, 309, 1415, 1666, 1711, 1800, 2054, 2435, 2856
TaqII GACCGA 4 cut(s) 343, 523, 1105, 2522
TatI WGTACW 6 cut(s) 1596, 1711, 1795, 1813, 2154, 2658
TfiI GAWTC 8 cut(s) 1010, 1106, 1391, 1632, 1975, 2044, 2381, 2599
Tru1I TTAA 6 cut(s) 903, 1911, 2082, 2552, 2858, 2925
Tru9I TTAA 6 cut(s) 903, 1911, 2082, 2552, 2858, 2925
TseFI GTSAC 7 cut(s) 170, 238, 457, 1095, 1415, 1705, 2930
TseI GCWGC 6 cut(s) 799, 988, 1543, 1768, 1925, 2970
Tsp45I GTSAC 7 cut(s) 170, 238, 457, 1095, 1415, 1705, 2930
TspGWI ACGGA 7 cut(s) 443, 449, 579, 794, 1394, 1607, 2666
VneI GTGCAC 1 cut(s) 2207
VpaK11BI GGWCC 7 cut(s) 479, 535, 781, 867, 922, 972, 2131
XapI RAATTY 4 cut(s) 880, 929, 2635, 2816
XbaI TCTAGA 2 cut(s) 1307, 2047
XceI RCATGY 2 cut(s) 2245, 2300
XcmI CCANNNNNNNNNTGG 3 cut(s) 1965, 2170, 2893
XmaJI CCTAGG 1 cut(s) 1199
XmiI GTMKAC 4 cut(s) 228, 632, 2706, 2830
XspI CTAG 6 cut(s) 1200, 1230, 1308, 2048, 2697, 2963
ZraI GACGTC 1 cut(s) 226
ZrmI AGTACT 1 cut(s) 2156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.