Rh4DG141300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004229
Physical Location & Seq
Forward (+)
27983398 .. 27984242
845 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG141300.1

Sequence Viewer

Length: 354 bp
ATGAGGACCATACTTACCAATTGCGTCGTTGGCTTGCTGTTGACGGTGGCATCTCTAGAAGCCGATGCTAAACGGTTTGTTTTGCATGACCGTCACTTGGTTCAAGTTAAAACTGATCCAAATATGGATCAAAACCCTGAGGTTCTTGCTAGTGGTGGTGCAAAAGATCAATTTCAGTTTCAGTCCCAGACCACAAGTAAGGGTAGGGATGCAAAGCATGATGATCATGACGAAGATGACGTGAACGAGATTCATGCTACAACCCAATGCATGCCAGATAAACCTCAAGGGAGGCCGCATTGTAAAACTACTGAGTCTAAAAGCTTTCATGAGCCTAAAAGCCTTCATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

117

Amino Acids

13.07

Weight (kDa)

6.02

Isoelectric Point (pI)

30.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 296
AclWI GGATC 2 cut(s) 110, 135
AfiI CCNNNNNNNGG 1 cut(s) 97
AgsI TTSAA 1 cut(s) 104
AjiI CACGTC 1 cut(s) 241
AluBI AGCT 1 cut(s) 324
AluI AGCT 1 cut(s) 324
AlwI GGATC 2 cut(s) 110, 135
AoxI GGCC 1 cut(s) 293
AspS9I GGNCC 1 cut(s) 6
AvaII GGWCC 1 cut(s) 6
AxyI CCTNAGG 1 cut(s) 138
BcgI CGANNNNNNTGC 2 cut(s) 236, 270
BclI TGATCA 1 cut(s) 223
BfaI CTAG 2 cut(s) 56, 150
BisI GCNGC 1 cut(s) 296
BlsI GCNGC 1 cut(s) 297
Bme18I GGWCC 1 cut(s) 6
BmgBI CACGTC 1 cut(s) 241
BmgT120I GGNCC 1 cut(s) 6
BmsI GCATC 3 cut(s) 55, 59, 199
BpuEI CTTGAG 1 cut(s) 270
Bsc4I CCNNNNNNNGG 1 cut(s) 97
Bse21I CCTNAGG 1 cut(s) 138
BseGI GGATG 1 cut(s) 214
BseLI CCNNNNNNNGG 1 cut(s) 97
BseMII CTCAG 2 cut(s) 129, 303
BshFI GGCC 1 cut(s) 295
BslFI GGGAC 1 cut(s) 169
BslI CCNNNNNNNGG 1 cut(s) 97
BsmFI GGGAC 1 cut(s) 169
BsnI GGCC 1 cut(s) 295
Bsp143I GATC 4 cut(s) 115, 127, 166, 223
BspACI CCGC 1 cut(s) 296
BspANI GGCC 1 cut(s) 295
BspCNI CTCAG 2 cut(s) 130, 304
BspHI TCATGA 2 cut(s) 226, 328
BspPI GGATC 2 cut(s) 110, 135
BssMI GATC 4 cut(s) 115, 127, 166, 223
Bst4CI ACNGT 3 cut(s) 46, 75, 92
BstC8I GCNNGC 2 cut(s) 35, 272
BstDEI CTNAG 2 cut(s) 138, 312
BstF5I GGATG 1 cut(s) 214
BstKTI GATC 4 cut(s) 118, 130, 169, 226
BstMBI GATC 4 cut(s) 115, 127, 166, 223
BstMWI GCNNNNNNNGC 1 cut(s) 30
BstNSI RCATGY 1 cut(s) 274
Bsu36I CCTNAGG 1 cut(s) 138
BsuRI GGCC 1 cut(s) 295
BtrI CACGTC 1 cut(s) 241
BtsCI GGATG 1 cut(s) 214
Cac8I GCNNGC 2 cut(s) 35, 272
CciI TCATGA 2 cut(s) 226, 328
Cfr13I GGNCC 1 cut(s) 6
CseI GACGC 1 cut(s) 13
CviAII CATG 8 cut(s) 86, 218, 227, 254, 271, 329, 347, 351
CviJI RGCY 6 cut(s) 33, 62, 295, 324, 334, 342
CviKI_1 RGCY 6 cut(s) 33, 62, 295, 324, 334, 342
DdeI CTNAG 2 cut(s) 138, 312
DpnI GATC 4 cut(s) 117, 129, 168, 225
DpnII GATC 4 cut(s) 115, 127, 166, 223
Eco47I GGWCC 1 cut(s) 6
Eco81I CCTNAGG 1 cut(s) 138
EcoT22I ATGCAT 2 cut(s) 272, 352
FaeI CATG 8 cut(s) 89, 221, 230, 257, 274, 332, 350, 354
FaqI GGGAC 1 cut(s) 169
FatI CATG 8 cut(s) 85, 217, 226, 253, 270, 328, 346, 350
FbaI TGATCA 1 cut(s) 223
Fnu4HI GCNGC 1 cut(s) 296
FokI GGATG 1 cut(s) 221
Fsp4HI GCNGC 1 cut(s) 296
FspBI CTAG 2 cut(s) 56, 150
GluI GCNGC 1 cut(s) 296
HaeIII GGCC 1 cut(s) 295
HgaI GACGC 1 cut(s) 13
Hin1II CATG 8 cut(s) 89, 221, 230, 257, 274, 332, 350, 354
HincII GTYRAC 1 cut(s) 42
HindII GTYRAC 1 cut(s) 42
HindIII AAGCTT 1 cut(s) 322
HinfI GANTC 2 cut(s) 250, 314
Hpy166II GTNNAC 2 cut(s) 42, 244
Hpy188III TCNNGA 3 cut(s) 56, 227, 329
Hpy8I GTNNAC 2 cut(s) 42, 244
Hpy99I CGWCG 1 cut(s) 29
HpyAV CCTTC 1 cut(s) 353
HpyCH4III ACNGT 3 cut(s) 46, 75, 92
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 5 cut(s) 85, 161, 212, 270, 350
HpyF10VI GCNNNNNNNGC 1 cut(s) 30
HpyF3I CTNAG 2 cut(s) 138, 312
HpySE526I ACGT 1 cut(s) 240
Hsp92II CATG 8 cut(s) 89, 221, 230, 257, 274, 332, 350, 354
Ksp22I TGATCA 1 cut(s) 223
Kzo9I GATC 4 cut(s) 115, 127, 166, 223
LpnPI CCDG 3 cut(s) 150, 200, 288
LweI GCATC 3 cut(s) 55, 59, 199
MaeI CTAG 2 cut(s) 56, 150
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 1 cut(s) 92
MalI GATC 4 cut(s) 117, 129, 168, 225
MboI GATC 4 cut(s) 115, 127, 166, 223
MboII GAAGA 1 cut(s) 245
MfeI CAATTG 1 cut(s) 19
MluCI AATT 2 cut(s) 19, 170
MlyI GAGTC 1 cut(s) 323
MnlI CCTC 3 cut(s) 133, 285, 294
Mph1103I ATGCAT 2 cut(s) 272, 352
MseI TTAA 1 cut(s) 108
MunI CAATTG 1 cut(s) 19
MwoI GCNNNNNNNGC 1 cut(s) 30
NdeII GATC 4 cut(s) 115, 127, 166, 223
NlaIII CATG 8 cut(s) 89, 221, 230, 257, 274, 332, 350, 354
NmuCI GTSAC 1 cut(s) 92
NsiI ATGCAT 2 cut(s) 272, 352
NspI RCATGY 1 cut(s) 274
PaeI GCATGC 1 cut(s) 274
PagI TCATGA 2 cut(s) 226, 328
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PfeI GAWTC 1 cut(s) 250
PkrI GCNGC 1 cut(s) 297
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
PspPI GGNCC 1 cut(s) 6
SaqAI TTAA 1 cut(s) 108
SatI GCNGC 1 cut(s) 296
Sau3AI GATC 4 cut(s) 115, 127, 166, 223
Sau96I GGNCC 1 cut(s) 6
SchI GAGTC 1 cut(s) 323
SetI ASST 4 cut(s) 144, 243, 286, 326
SfaNI GCATC 3 cut(s) 55, 59, 199
SinI GGWCC 1 cut(s) 6
SmlI CTYRAG 1 cut(s) 285
SmoI CTYRAG 1 cut(s) 285
SphI GCATGC 1 cut(s) 274
Sse9I AATT 2 cut(s) 19, 170
SsiI CCGC 1 cut(s) 296
SspMI CTAG 2 cut(s) 56, 150
TaaI ACNGT 3 cut(s) 46, 75, 92
TaiI ACGT 1 cut(s) 243
TasI AATT 2 cut(s) 19, 170
TauI GCSGC 1 cut(s) 298
TfiI GAWTC 1 cut(s) 250
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TseFI GTSAC 1 cut(s) 92
Tsp45I GTSAC 1 cut(s) 92
TspDTI ATGAA 3 cut(s) 242, 317, 335
VpaK11BI GGWCC 1 cut(s) 6
XbaI TCTAGA 1 cut(s) 55
XceI RCATGY 1 cut(s) 274
XspI CTAG 2 cut(s) 56, 150
Zsp2I ATGCAT 2 cut(s) 272, 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.