Rh5AG496500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0004229
Physical Location & Seq
Forward (+)
84343021 .. 84343608
588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG496500.1

Sequence Viewer

Length: 456 bp
ATGCAACCTTTGGAATCCCTTCTGTCATATAGTTGGACATTTCTCGAAACCTGCAGATTCATTACGTACCAACATGAGGACCAATGGACCATACTTGTCATATGCGTTATTGGCTTTGTGGTGATGCTGGCATCTCTGCAAGCAGATGCTAAACGGTTTGTTTTGAATGACCGTCACTTGGTTCAAGTTAAGGATGAGCCAACTCTGCGTCGAAGCGCCGAGGCTCTTGCTAGTGGCGGTGCAAAAGATCAATCTCAGTCTCAGCCCCGGACCACAAACAAGAGTAGCAGCAACAGCGATGCTAAGCATGATGATCATGTCGACGATGATTCAAACGAGACTTATGGTAAGTCCGGATATGGCTCTTCCGAAAGTGACTCTCGCCATTTTTTTTATTGGCGATACATACATATGAGATCCCCCAGGATTCCTGGTGCTGGCAATGGACCCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

151

Amino Acids

17.13

Weight (kDa)

6.13

Isoelectric Point (pI)

47.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 59
AccI GTMKAC 1 cut(s) 321
AccIII TCCGGA 1 cut(s) 353
AciI CCGC 1 cut(s) 237
AclWI GGATC 1 cut(s) 411
AfaI GTAC 1 cut(s) 68
AfiI CCNNNNNNNGG 3 cut(s) 76, 178, 437
AgsI TTSAA 3 cut(s) 166, 185, 333
AjnI CCWGG 2 cut(s) 422, 430
Alw26I GTCTC 2 cut(s) 264, 332
AlwI GGATC 1 cut(s) 411
Aor13HI TCCGGA 1 cut(s) 353
ApeKI GCWGC 1 cut(s) 288
Asp700I GAANNNNTTC 1 cut(s) 18
AspLEI GCGC 1 cut(s) 218
AspS9I GGNCC 4 cut(s) 79, 87, 270, 446
AsuC2I CCSGG 1 cut(s) 268
AsuHPI GGTGA 1 cut(s) 133
AvaII GGWCC 4 cut(s) 79, 87, 270, 446
BbvI GCAGC 1 cut(s) 300
BcgI CGANNNNNNTGC 2 cut(s) 209, 243
BciT130I CCWGG 2 cut(s) 424, 432
BclI TGATCA 1 cut(s) 313
BcnI CCSGG 1 cut(s) 268
BcoDI GTCTC 2 cut(s) 264, 332
BfaI CTAG 1 cut(s) 231
BfmI CTRYAG 1 cut(s) 52
BfoI RGCGCY 1 cut(s) 219
BfuAI ACCTGC 1 cut(s) 59
BisI GCNGC 1 cut(s) 289
BlpI GCTNAGC 1 cut(s) 303
BlsI GCNGC 1 cut(s) 290
Bme1390I CCNGG 3 cut(s) 268, 424, 432
Bme18I GGWCC 4 cut(s) 79, 87, 270, 446
BmgT120I GGNCC 4 cut(s) 79, 87, 270, 446
BmiI GGNNCC 1 cut(s) 448
BmrFI CCNGG 3 cut(s) 268, 424, 432
BmsI GCATC 4 cut(s) 114, 136, 140, 289
Bpu1102I GCTNAGC 1 cut(s) 303
BpuMI CCSGG 1 cut(s) 268
BsaAI YACGTR 1 cut(s) 66
BsaJI CCNNGG 3 cut(s) 219, 266, 422
BsaWI WCCGGW 1 cut(s) 353
Bsc4I CCNNNNNNNGG 3 cut(s) 76, 178, 437
Bse3DI GCAATG 1 cut(s) 448
BseAI TCCGGA 1 cut(s) 353
BseBI CCWGG 2 cut(s) 424, 432
BseDI CCNNGG 3 cut(s) 219, 266, 422
BseGI GGATG 1 cut(s) 199
BseLI CCNNNNNNNGG 3 cut(s) 76, 178, 437
BseMI GCAATG 1 cut(s) 448
BseMII CTCAG 2 cut(s) 269, 275
BseXI GCAGC 1 cut(s) 300
BsiSI CCGG 2 cut(s) 268, 354
BslI CCNNNNNNNGG 3 cut(s) 76, 178, 437
BsmAI GTCTC 2 cut(s) 264, 332
Bsp13I TCCGGA 1 cut(s) 353
Bsp143I GATC 3 cut(s) 247, 313, 416
Bsp1720I GCTNAGC 1 cut(s) 303
BspACI CCGC 1 cut(s) 237
BspCNI CTCAG 2 cut(s) 268, 274
BspEI TCCGGA 1 cut(s) 353
BspLI GGNNCC 1 cut(s) 448
BspMAI CTGCAG 1 cut(s) 56
BspMI ACCTGC 1 cut(s) 59
BspPI GGATC 1 cut(s) 411
BspQI GCTCTTC 1 cut(s) 370
BsrDI GCAATG 1 cut(s) 448
BssECI CCNNGG 3 cut(s) 219, 266, 422
BssMI GATC 3 cut(s) 247, 313, 416
Bst2UI CCWGG 2 cut(s) 424, 432
Bst4CI ACNGT 2 cut(s) 156, 173
Bst6I CTCTTC 1 cut(s) 370
BstBAI YACGTR 1 cut(s) 66
BstC8I GCNNGC 3 cut(s) 129, 141, 439
BstDEI CTNAG 3 cut(s) 255, 261, 303
BstF5I GGATG 1 cut(s) 199
BstH2I RGCGCY 1 cut(s) 219
BstHHI GCGC 1 cut(s) 218
BstKTI GATC 3 cut(s) 250, 316, 419
BstMAI GTCTC 2 cut(s) 264, 332
BstMBI GATC 3 cut(s) 247, 313, 416
BstMWI GCNNNNNNNGC 3 cut(s) 111, 205, 294
BstNI CCWGG 2 cut(s) 424, 432
BstSCI CCNGG 3 cut(s) 266, 422, 430
BstSFI CTRYAG 1 cut(s) 52
BstSNI TACGTA 1 cut(s) 66
BstV1I GCAGC 1 cut(s) 300
BstX2I RGATCY 1 cut(s) 416
BstYI RGATCY 1 cut(s) 416
BtgZI GCGATG 1 cut(s) 312
BtsCI GGATG 1 cut(s) 199
BveI ACCTGC 1 cut(s) 59
Cac8I GCNNGC 3 cut(s) 129, 141, 439
CfoI GCGC 1 cut(s) 218
Cfr13I GGNCC 4 cut(s) 79, 87, 270, 446
CseI GACGC 1 cut(s) 197
Csp6I GTAC 1 cut(s) 67
CviAII CATG 3 cut(s) 74, 308, 317
CviJI RGCY 5 cut(s) 114, 199, 224, 265, 363
CviKI_1 RGCY 5 cut(s) 114, 199, 224, 265, 363
CviQI GTAC 1 cut(s) 67
DdeI CTNAG 3 cut(s) 255, 261, 303
DpnI GATC 3 cut(s) 249, 315, 418
DpnII GATC 3 cut(s) 247, 313, 416
Eam1104I CTCTTC 1 cut(s) 370
EarI CTCTTC 1 cut(s) 370
Eco105I TACGTA 1 cut(s) 66
Eco47I GGWCC 4 cut(s) 79, 87, 270, 446
EcoRII CCWGG 2 cut(s) 422, 430
FaeI CATG 3 cut(s) 77, 311, 320
FatI CATG 3 cut(s) 73, 307, 316
FauNDI CATATG 2 cut(s) 101, 411
FbaI TGATCA 1 cut(s) 313
FblI GTMKAC 1 cut(s) 321
Fnu4HI GCNGC 1 cut(s) 289
FokI GGATG 1 cut(s) 206
Fsp4HI GCNGC 1 cut(s) 289
FspBI CTAG 1 cut(s) 231
GlaI GCGC 1 cut(s) 217
GluI GCNGC 1 cut(s) 289
HaeII RGCGCY 1 cut(s) 219
HapII CCGG 2 cut(s) 268, 354
HgaI GACGC 1 cut(s) 197
HhaI GCGC 1 cut(s) 218
Hin1II CATG 3 cut(s) 77, 311, 320
Hin6I GCGC 1 cut(s) 216
HinP1I GCGC 1 cut(s) 216
HincII GTYRAC 1 cut(s) 322
HindII GTYRAC 1 cut(s) 322
HinfI GANTC 5 cut(s) 14, 57, 329, 377, 427
HpaII CCGG 2 cut(s) 268, 354
HphI GGTGA 1 cut(s) 133
Hpy166II GTNNAC 1 cut(s) 322
Hpy188I TCNGA 1 cut(s) 370
Hpy188III TCNNGA 2 cut(s) 44, 354
Hpy8I GTNNAC 1 cut(s) 322
Hpy99I CGWCG 2 cut(s) 213, 326
HpyAV CCTTC 1 cut(s) 29
HpyCH4III ACNGT 2 cut(s) 156, 173
HpyCH4IV ACGT 1 cut(s) 65
HpyCH4V TGCA 4 cut(s) 4, 54, 139, 242
HpyF10VI GCNNNNNNNGC 3 cut(s) 111, 205, 294
HpyF3I CTNAG 3 cut(s) 255, 261, 303
HpySE526I ACGT 1 cut(s) 65
Hsp92II CATG 3 cut(s) 77, 311, 320
HspAI GCGC 1 cut(s) 216
Kpn2I TCCGGA 1 cut(s) 353
Ksp22I TGATCA 1 cut(s) 313
Kzo9I GATC 3 cut(s) 247, 313, 416
LguI GCTCTTC 1 cut(s) 370
LpnPI CCDG 9 cut(s) 64, 113, 281, 367, 409, 417, 423, 436, 444
Lsp1109I GCAGC 1 cut(s) 300
LweI GCATC 4 cut(s) 114, 136, 140, 289
MaeI CTAG 1 cut(s) 231
MaeII ACGT 1 cut(s) 65
MaeIII GTNAC 2 cut(s) 173, 374
MalI GATC 3 cut(s) 249, 315, 418
MboI GATC 3 cut(s) 247, 313, 416
MboII GAAGA 1 cut(s) 357
MflI RGATCY 1 cut(s) 416
MlyI GAGTC 1 cut(s) 371
MmeI TCCRAC 1 cut(s) 14
MnlI CCTC 2 cut(s) 70, 214
MroI TCCGGA 1 cut(s) 353
MroXI GAANNNNTTC 1 cut(s) 18
MseI TTAA 1 cut(s) 189
MslI CAYNNNNRTG 1 cut(s) 410
MspI CCGG 2 cut(s) 268, 354
MspR9I CCNGG 3 cut(s) 268, 424, 432
MvaI CCWGG 2 cut(s) 424, 432
MwoI GCNNNNNNNGC 3 cut(s) 111, 205, 294
NciI CCSGG 1 cut(s) 268
NdeI CATATG 2 cut(s) 101, 411
NdeII GATC 3 cut(s) 247, 313, 416
NlaIII CATG 3 cut(s) 77, 311, 320
NlaIV GGNNCC 1 cut(s) 448
NmeAIII GCCGAG 1 cut(s) 244
NmuCI GTSAC 2 cut(s) 173, 374
PciSI GCTCTTC 1 cut(s) 370
PdmI GAANNNNTTC 1 cut(s) 18
PfeI GAWTC 4 cut(s) 14, 57, 329, 427
PkrI GCNGC 1 cut(s) 290
PleI GAGTC 1 cut(s) 371
PpsI GAGTC 1 cut(s) 371
Ppu21I YACGTR 1 cut(s) 66
Psp6I CCWGG 2 cut(s) 422, 430
PspGI CCWGG 2 cut(s) 422, 430
PspN4I GGNNCC 1 cut(s) 448
PspPI GGNCC 4 cut(s) 79, 87, 270, 446
PstI CTGCAG 1 cut(s) 56
PsuI RGATCY 1 cut(s) 416
RsaI GTAC 1 cut(s) 68
RsaNI GTAC 1 cut(s) 67
RseI CAYNNNNRTG 1 cut(s) 410
SalI GTCGAC 1 cut(s) 320
SapI GCTCTTC 1 cut(s) 370
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 1 cut(s) 289
Sau3AI GATC 3 cut(s) 247, 313, 416
Sau96I GGNCC 4 cut(s) 79, 87, 270, 446
SchI GAGTC 1 cut(s) 371
ScrFI CCNGG 3 cut(s) 268, 424, 432
SetI ASST 3 cut(s) 10, 53, 68
SfaNI GCATC 4 cut(s) 114, 136, 140, 289
SfcI CTRYAG 1 cut(s) 52
SinI GGWCC 4 cut(s) 79, 87, 270, 446
SmiMI CAYNNNNRTG 1 cut(s) 410
SnaBI TACGTA 1 cut(s) 66
SsiI CCGC 1 cut(s) 237
SspMI CTAG 1 cut(s) 231
StyD4I CCNGG 3 cut(s) 266, 422, 430
TaaI ACNGT 2 cut(s) 156, 173
TaiI ACGT 1 cut(s) 68
TaqI TCGA 3 cut(s) 45, 211, 321
TfiI GAWTC 4 cut(s) 14, 57, 329, 427
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TseFI GTSAC 2 cut(s) 173, 374
TseI GCWGC 1 cut(s) 288
Tsp45I GTSAC 2 cut(s) 173, 374
TspDTI ATGAA 1 cut(s) 49
VpaK11BI GGWCC 4 cut(s) 79, 87, 270, 446
XmiI GTMKAC 1 cut(s) 321
XmnI GAANNNNTTC 1 cut(s) 18
XspI CTAG 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.