MD03G1039400.v1.1

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009341
Physical Location & Seq
Reverse (-)
3135113 .. 3136636
1524 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1039400.v1.1.491

Sequence Viewer

Length: 675 bp
ATGCAGTCCAACGGAGCTCCTACAGCCATGGAAGTCGAAGCAGGTTCATCTGCACCAAATTCATTGCCTGGGACTTTACCACCCAAGCCATCGTTTGCGCCTTTGAAGGCTCATGAGATGTCAGATGGACAAGTCCAGTTCCGGAAAGTCTCTGTTCCGCCGCATCGTTATTCTCCCCTCAAGAAAGCATGGATGGACATATACACCCCAATTTATGAGCAGATGAAGATAGACATTCGTATGAATCTCAAGGGTCGCAAAGTTGAGTTGAAGACTAGAGCTGACACACCCGATGTAAGTAATCTACAGAAGTGCGCTGATTTTGTTCAGGCTTTCATGCTGGGTTTTGATGTTATAGACGCCGTTGCCCTTTTGCGGATGGATGAGCTCTATGTAGAGTCTTTTGAGATCAAGGACGTTAAAACACTCCGTGGGGAGCACTTGTCTCGTGCTATAGGAAGGCTTTCCGGTAAGGGTGGTAAAACAAAGTTTGCAATCGAGAATGCTACGAAGACAAGGATTGTGATTGCTGACACCAAGATTCACATACTGGGATCTTTTGCAAATATTAAGGTTGCAAGGGATTCTCTTTGCAGCCTTATCTTAGGGTCCCCTGCCGGAAAAGTGTATTCGAAATTAAGAGCAGTTACTGCTAGATTGGCGGAAAGGTTTTAA

Protein Analysis

225

Amino Acids

24.71

Weight (kDa)

9.69

Isoelectric Point (pI)

29.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_PNO1_2nd PF22891 134 - 217 3.9e-36 Eukaryotic type KH-domain (KH-domain type I)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 32
AccIII TCCGGA 1 cut(s) 141
AciI CCGC 4 cut(s) 158, 161, 376, 662
AclWI GGATC 1 cut(s) 562
AcsI RAATTY 1 cut(s) 58
AcyI GRCGYC 1 cut(s) 360
AdeI CACNNNGTG 1 cut(s) 431
AfiI CCNNNNNNNGG 1 cut(s) 375
AgsI TTSAA 2 cut(s) 106, 271
AjnI CCWGG 1 cut(s) 67
AluBI AGCT 3 cut(s) 17, 281, 388
AluI AGCT 3 cut(s) 17, 281, 388
Alw21I GWGCWC 3 cut(s) 19, 390, 441
Alw26I GTCTC 2 cut(s) 154, 450
AlwI GGATC 1 cut(s) 562
AlwNI CAGNNNCTG 1 cut(s) 650
Aor13HI TCCGGA 1 cut(s) 141
ApeKI GCWGC 1 cut(s) 594
ApoI RAATTY 1 cut(s) 58
Asp700I GAANNNNTTC 1 cut(s) 463
AspLEI GCGC 2 cut(s) 100, 317
AspS9I GGNCC 1 cut(s) 609
AsuII TTCGAA 1 cut(s) 632
AvaII GGWCC 1 cut(s) 609
BanII GRGCYC 2 cut(s) 19, 390
BauI CACGAG 1 cut(s) 447
BbsI GAAGAC 2 cut(s) 278, 518
Bbv12I GWGCWC 3 cut(s) 19, 390, 441
BbvI GCAGC 1 cut(s) 606
BccI CCATC 4 cut(s) 97, 119, 187, 373
BceAI ACGGC 1 cut(s) 347
BcgI CGANNNNNNTGC 2 cut(s) 428, 462
BciT130I CCWGG 1 cut(s) 69
BcoDI GTCTC 2 cut(s) 154, 450
BfaI CTAG 2 cut(s) 276, 654
BfmI CTRYAG 3 cut(s) 21, 305, 453
BfuAI ACCTGC 1 cut(s) 32
BisI GCNGC 2 cut(s) 161, 595
BlsI GCNGC 2 cut(s) 162, 596
Bme1390I CCNGG 1 cut(s) 69
Bme18I GGWCC 1 cut(s) 609
BmgT120I GGNCC 1 cut(s) 609
BmiI GGNNCC 2 cut(s) 610, 611
BmrFI CCNGG 1 cut(s) 69
BmrI ACTGGG 1 cut(s) 560
BmsI GCATC 1 cut(s) 172
BmuI ACTGGG 1 cut(s) 560
BpiI GAAGAC 2 cut(s) 278, 518
Bpu14I TTCGAA 1 cut(s) 632
BpuEI CTTGAG 2 cut(s) 164, 233
BsaHI GRCGYC 1 cut(s) 360
BsaJI CCNNGG 3 cut(s) 27, 68, 430
BsaWI WCCGGW 2 cut(s) 141, 467
BsaXI ACNNNNNCTCC 2 cut(s) 428, 458
Bsc4I CCNNNNNNNGG 1 cut(s) 375
Bse1I ACTGG 2 cut(s) 136, 555
Bse3DI GCAATG 1 cut(s) 62
BseAI TCCGGA 1 cut(s) 141
BseBI CCWGG 1 cut(s) 69
BseDI CCNNGG 3 cut(s) 27, 68, 430
BseGI GGATG 3 cut(s) 198, 384, 388
BseLI CCNNNNNNNGG 1 cut(s) 375
BseMI GCAATG 1 cut(s) 62
BseNI ACTGG 2 cut(s) 136, 555
BseXI GCAGC 1 cut(s) 606
BseYI CCCAGC 1 cut(s) 340
BsgI GTGCAG 1 cut(s) 36
BsiHKAI GWGCWC 3 cut(s) 19, 390, 441
BsiSI CCGG 3 cut(s) 142, 468, 618
BslFI GGGAC 2 cut(s) 85, 595
BslI CCNNNNNNNGG 1 cut(s) 375
BsmAI GTCTC 2 cut(s) 154, 450
BsmFI GGGAC 2 cut(s) 85, 595
BsmI GAATGC 1 cut(s) 508
Bsp119I TTCGAA 1 cut(s) 632
Bsp1286I GDGCHC 3 cut(s) 19, 390, 441
Bsp13I TCCGGA 1 cut(s) 141
Bsp143I GATC 2 cut(s) 408, 554
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 4 cut(s) 158, 161, 376, 662
BspEI TCCGGA 1 cut(s) 141
BspHI TCATGA 1 cut(s) 112
BspLI GGNNCC 2 cut(s) 610, 611
BspMI ACCTGC 1 cut(s) 32
BspPI GGATC 1 cut(s) 562
BspT104I TTCGAA 1 cut(s) 632
BsrDI GCAATG 1 cut(s) 62
BsrI ACTGG 2 cut(s) 136, 555
BssECI CCNNGG 3 cut(s) 27, 68, 430
BssMI GATC 2 cut(s) 408, 554
BssNI GRCGYC 1 cut(s) 360
BssSI CACGAG 1 cut(s) 447
BssT1I CCWWGG 1 cut(s) 27
Bst2BI CACGAG 1 cut(s) 447
Bst2UI CCWGG 1 cut(s) 69
BstACI GRCGYC 1 cut(s) 360
BstAPI GCANNNNNTGC 1 cut(s) 650
BstBI TTCGAA 1 cut(s) 632
BstDEI CTNAG 1 cut(s) 604
BstDSI CCRYGG 2 cut(s) 27, 430
BstF5I GGATG 3 cut(s) 198, 384, 388
BstHHI GCGC 2 cut(s) 100, 317
BstKTI GATC 2 cut(s) 411, 557
BstMAI GTCTC 2 cut(s) 154, 450
BstMBI GATC 2 cut(s) 408, 554
BstMWI GCNNNNNNNGC 3 cut(s) 23, 650, 659
BstNI CCWGG 1 cut(s) 69
BstSCI CCNGG 1 cut(s) 67
BstSFI CTRYAG 3 cut(s) 21, 305, 453
BstV1I GCAGC 1 cut(s) 606
BstV2I GAAGAC 2 cut(s) 278, 518
BstX2I RGATCY 1 cut(s) 554
BstYI RGATCY 1 cut(s) 554
BtgI CCRYGG 2 cut(s) 27, 430
BtsCI GGATG 3 cut(s) 198, 384, 388
BveI ACCTGC 1 cut(s) 32
CaiI CAGNNNCTG 1 cut(s) 650
CciI TCATGA 1 cut(s) 112
CfoI GCGC 2 cut(s) 100, 317
Cfr13I GGNCC 1 cut(s) 609
CseI GACGC 1 cut(s) 368
CviAII CATG 4 cut(s) 28, 113, 189, 337
CviJI RGCY 9 cut(s) 17, 26, 88, 110, 281, 332, 388, 463, 597
CviKI_1 RGCY 9 cut(s) 17, 26, 88, 110, 281, 332, 388, 463, 597
DdeI CTNAG 1 cut(s) 604
DpnI GATC 2 cut(s) 410, 556
DpnII GATC 2 cut(s) 408, 554
DraIII CACNNNGTG 1 cut(s) 431
EciI GGCGGA 1 cut(s) 147
Ecl136II GAGCTC 2 cut(s) 17, 388
Eco130I CCWWGG 1 cut(s) 27
Eco24I GRGCYC 2 cut(s) 19, 390
Eco47I GGWCC 1 cut(s) 609
Eco53kI GAGCTC 2 cut(s) 17, 388
EcoICRI GAGCTC 2 cut(s) 17, 388
EcoO109I RGGNCCY 1 cut(s) 609
EcoRII CCWGG 1 cut(s) 67
EcoT14I CCWWGG 1 cut(s) 27
EcoT38I GRGCYC 2 cut(s) 19, 390
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 4 cut(s) 31, 116, 192, 340
FaqI GGGAC 2 cut(s) 85, 595
FatI CATG 4 cut(s) 27, 112, 188, 336
Fnu4HI GCNGC 2 cut(s) 161, 595
FokI GGATG 3 cut(s) 205, 391, 395
FriOI GRGCYC 2 cut(s) 19, 390
Fsp4HI GCNGC 2 cut(s) 161, 595
FspBI CTAG 2 cut(s) 276, 654
GlaI GCGC 2 cut(s) 99, 316
GluI GCNGC 2 cut(s) 161, 595
GsaI CCCAGC 1 cut(s) 344
HapII CCGG 3 cut(s) 142, 468, 618
HgaI GACGC 1 cut(s) 368
HhaI GCGC 2 cut(s) 100, 317
Hin1I GRCGYC 1 cut(s) 360
Hin1II CATG 4 cut(s) 31, 116, 192, 340
Hin6I GCGC 2 cut(s) 98, 315
HinP1I GCGC 2 cut(s) 98, 315
HinfI GANTC 4 cut(s) 244, 398, 541, 584
HpaII CCGG 3 cut(s) 142, 468, 618
Hpy188I TCNGA 1 cut(s) 124
Hpy188III TCNNGA 4 cut(s) 113, 142, 181, 499
HpyAV CCTTC 2 cut(s) 100, 453
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 6 cut(s) 4, 53, 494, 563, 578, 594
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 650, 659
HpyF3I CTNAG 1 cut(s) 604
HpySE526I ACGT 1 cut(s) 417
Hsp92I GRCGYC 1 cut(s) 360
Hsp92II CATG 4 cut(s) 31, 116, 192, 340
HspAI GCGC 2 cut(s) 98, 315
KflI GGGWCCC 1 cut(s) 609
Kpn2I TCCGGA 1 cut(s) 141
Kzo9I GATC 2 cut(s) 408, 554
LmnI GCTCC 3 cut(s) 14, 22, 436
Lsp1109I GCAGC 1 cut(s) 606
LweI GCATC 1 cut(s) 172
MaeI CTAG 2 cut(s) 276, 654
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 1 cut(s) 646
MalI GATC 2 cut(s) 410, 556
MboI GATC 2 cut(s) 408, 554
MboII GAAGA 3 cut(s) 238, 283, 523
MflI RGATCY 1 cut(s) 554
MhlI GDGCHC 3 cut(s) 19, 390, 441
MluCI AATT 3 cut(s) 58, 210, 635
MlyI GAGTC 1 cut(s) 407
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 1 cut(s) 188
MroI TCCGGA 1 cut(s) 141
MroXI GAANNNNTTC 1 cut(s) 463
MseI TTAA 4 cut(s) 420, 570, 638, 673
MslI CAYNNNNRTG 1 cut(s) 239
MspI CCGG 3 cut(s) 142, 468, 618
MspR9I CCNGG 1 cut(s) 69
Mva1269I GAATGC 1 cut(s) 508
MvaI CCWGG 1 cut(s) 69
MwoI GCNNNNNNNGC 3 cut(s) 23, 650, 659
NcoI CCATGG 1 cut(s) 27
NdeII GATC 2 cut(s) 408, 554
NlaIII CATG 4 cut(s) 31, 116, 192, 340
NlaIV GGNNCC 2 cut(s) 610, 611
NspV TTCGAA 1 cut(s) 632
PagI TCATGA 1 cut(s) 112
PctI GAATGC 1 cut(s) 508
PdmI GAANNNNTTC 1 cut(s) 463
PfeI GAWTC 3 cut(s) 244, 541, 584
PkrI GCNGC 2 cut(s) 162, 596
PleI GAGTC 1 cut(s) 406
PpsI GAGTC 1 cut(s) 406
PpuMI RGGWCCY 1 cut(s) 609
Psp124BI GAGCTC 2 cut(s) 19, 390
Psp5II RGGWCCY 1 cut(s) 609
Psp6I CCWGG 1 cut(s) 67
PspFI CCCAGC 1 cut(s) 340
PspGI CCWGG 1 cut(s) 67
PspN4I GGNNCC 2 cut(s) 610, 611
PspPI GGNCC 1 cut(s) 609
PspPPI RGGWCCY 1 cut(s) 609
PstNI CAGNNNCTG 1 cut(s) 650
PsuI RGATCY 1 cut(s) 554
RseI CAYNNNNRTG 1 cut(s) 239
SacI GAGCTC 2 cut(s) 19, 390
SaqAI TTAA 4 cut(s) 420, 570, 638, 673
SatI GCNGC 2 cut(s) 161, 595
Sau3AI GATC 2 cut(s) 408, 554
Sau96I GGNCC 1 cut(s) 609
SchI GAGTC 1 cut(s) 407
ScrFI CCNGG 1 cut(s) 69
SduI GDGCHC 3 cut(s) 19, 390, 441
SetI ASST 7 cut(s) 19, 46, 283, 390, 420, 576, 671
SfaNI GCATC 1 cut(s) 172
SfcI CTRYAG 3 cut(s) 21, 305, 453
SfuI TTCGAA 1 cut(s) 632
SinI GGWCC 1 cut(s) 609
SmiMI CAYNNNNRTG 1 cut(s) 239
SmlI CTYRAG 2 cut(s) 179, 248
SmoI CTYRAG 2 cut(s) 179, 248
Sse9I AATT 3 cut(s) 58, 210, 635
SsiI CCGC 4 cut(s) 158, 161, 376, 662
SspI AATATT 1 cut(s) 568
SspMI CTAG 2 cut(s) 276, 654
SstI GAGCTC 2 cut(s) 19, 390
StyD4I CCNGG 1 cut(s) 67
StyI CCWWGG 1 cut(s) 27
TaiI ACGT 1 cut(s) 420
TaqI TCGA 3 cut(s) 36, 498, 632
TasI AATT 3 cut(s) 58, 210, 635
TauI GCSGC 1 cut(s) 163
TfiI GAWTC 3 cut(s) 244, 541, 584
Tru1I TTAA 4 cut(s) 420, 570, 638, 673
Tru9I TTAA 4 cut(s) 420, 570, 638, 673
TseI GCWGC 1 cut(s) 594
TspDTI ATGAA 5 cut(s) 36, 51, 239, 257, 325
TspGWI ACGGA 2 cut(s) 27, 419
VpaK11BI GGWCC 1 cut(s) 609
XapI RAATTY 1 cut(s) 58
XmnI GAANNNNTTC 1 cut(s) 463
XspI CTAG 2 cut(s) 276, 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.