Rw0G014510

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00650
OG0009341
Physical Location & Seq
Reverse (-)
114447 .. 116143
1697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G014510.1

Sequence Viewer

Length: 675 bp
ATGCAGTCCAATGAAGCTCCTACTTCCATGGAAGTCGAAGCAGTGGCAAATGGAGCCAGTTCAGCATCTGGGGCACTGCCTGCAAAGCCAAGTTTTGAGCCTTTTAAGGCTCATGAGATGTCAGATGGGCAAGTTCAGTTCCGGAAGGTCTCTGTTCCACCGCATCGTTATACGCCACTGAAGAAAGCATGGATGGAAATATACACCCCAATTTACGAGCAGATGAAGATAGACATCCGTATGAATCTCAAGGGCCGCAAAGTGGAGTTGAAGACCAGACGTGACACACCTGACATTAGTCATCTGCAGAAGTGTGCTGATTTTGTCCAGGCTTTCATGCTGGGGTTTGATGTCATAGATGCCATTGCCCTTCTACGTTTGGATGAGCTTTATGTGGAGTCCTTTGAGATCAAGGATGTCAAAACACTTCGTGGGGAGCACTTGTCTCGCGCTATAGGAAGGCTGTCTGGGAAAGGGGGCAGAACAAAATTCGCGATTGAGAATGCTACAAAGACTAGGATTGTGATTGCAGACACCAAGATCCACATATTGGGATCTTTTGCAAACATAAAGGTTGCTAGGGACTCTCTTTGCAACCTTATCTTAGGATCCCCTGCTACTAAAGTGTATTCAAAACTAAGAGCAGTAACTGCTAGATTGGCAGAAAGGTTTTAG

Protein Analysis

224

Amino Acids

25.01

Weight (kDa)

9.64

Isoelectric Point (pI)

30.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_PNO1_2nd PF22891 134 - 217 1.7e-35 Eukaryotic type KH-domain (KH-domain type I)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 550
AccII CGCG 2 cut(s) 450, 494
AccIII TCCGGA 1 cut(s) 141
AciI CCGC 2 cut(s) 161, 256
AclWI GGATC 4 cut(s) 535, 562, 603, 616
AcsI RAATTY 1 cut(s) 488
AcuI CTGAAG 1 cut(s) 200
AdeI CACNNNGTG 1 cut(s) 431
AfiI CCNNNNNNNGG 2 cut(s) 262, 550
AgsI TTSAA 2 cut(s) 271, 633
AjiI CACGTC 1 cut(s) 281
AjnI CCWGG 1 cut(s) 327
AluBI AGCT 2 cut(s) 17, 388
AluI AGCT 2 cut(s) 17, 388
Alw21I GWGCWC 1 cut(s) 441
Alw26I GTCTC 2 cut(s) 154, 450
AlwI GGATC 4 cut(s) 535, 562, 603, 616
AlwNI CAGNNNCTG 2 cut(s) 68, 650
Aor13HI TCCGGA 1 cut(s) 141
AoxI GGCC 1 cut(s) 253
ApoI RAATTY 1 cut(s) 488
AspLEI GCGC 1 cut(s) 452
AspS9I GGNCC 1 cut(s) 253
BaeGI GKGCMC 1 cut(s) 76
BamHI GGATCC 1 cut(s) 608
BbsI GAAGAC 1 cut(s) 278
Bbv12I GWGCWC 1 cut(s) 441
BccI CCATC 2 cut(s) 119, 187
BcgI CGANNNNNNTGC 2 cut(s) 428, 462
BciT130I CCWGG 1 cut(s) 329
BcoDI GTCTC 2 cut(s) 154, 450
BfaI CTAG 3 cut(s) 516, 579, 654
BfmI CTRYAG 2 cut(s) 305, 453
BisI GCNGC 1 cut(s) 256
BlsI GCNGC 1 cut(s) 257
Bme1390I CCNGG 1 cut(s) 329
BmgBI CACGTC 1 cut(s) 281
BmgT120I GGNCC 1 cut(s) 253
BmiI GGNNCC 2 cut(s) 55, 610
BmrFI CCNGG 1 cut(s) 329
BmsI GCATC 3 cut(s) 74, 172, 349
BoxI GACNNNNGTC 1 cut(s) 297
BpiI GAAGAC 1 cut(s) 278
BpuEI CTTGAG 1 cut(s) 233
BsaBI GATNNNNATC 1 cut(s) 233
BsaI GGTCTC 1 cut(s) 154
BsaJI CCNNGG 1 cut(s) 27
BsaWI WCCGGW 1 cut(s) 141
BsaXI ACNNNNNCTCC 2 cut(s) 428, 458
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 550
Bse1I ACTGG 1 cut(s) 57
Bse3DI GCAATG 1 cut(s) 363
Bse8I GATNNNNATC 1 cut(s) 233
BseAI TCCGGA 1 cut(s) 141
BseBI CCWGG 1 cut(s) 329
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 4 cut(s) 198, 234, 388, 421
BseJI GATNNNNATC 1 cut(s) 233
BseLI CCNNNNNNNGG 2 cut(s) 262, 550
BseMI GCAATG 1 cut(s) 363
BseNI ACTGG 1 cut(s) 57
BseSI GKGCMC 1 cut(s) 76
BseYI CCCAGC 1 cut(s) 340
Bsh1236I CGCG 2 cut(s) 450, 494
BshFI GGCC 1 cut(s) 255
BsiHKAI GWGCWC 1 cut(s) 441
BsiSI CCGG 1 cut(s) 142
BslFI GGGAC 1 cut(s) 596
BslI CCNNNNNNNGG 2 cut(s) 262, 550
BsmAI GTCTC 2 cut(s) 154, 450
BsmFI GGGAC 1 cut(s) 596
BsmI GAATGC 1 cut(s) 508
BsnI GGCC 1 cut(s) 255
Bso31I GGTCTC 1 cut(s) 154
Bsp1286I GDGCHC 2 cut(s) 76, 441
Bsp13I TCCGGA 1 cut(s) 141
Bsp143I GATC 4 cut(s) 408, 540, 554, 608
Bsp19I CCATGG 1 cut(s) 27
Bsp68I TCGCGA 1 cut(s) 494
BspACI CCGC 2 cut(s) 161, 256
BspANI GGCC 1 cut(s) 255
BspEI TCCGGA 1 cut(s) 141
BspFNI CGCG 2 cut(s) 450, 494
BspHI TCATGA 1 cut(s) 112
BspLI GGNNCC 2 cut(s) 55, 610
BspMAI CTGCAG 1 cut(s) 309
BspPI GGATC 4 cut(s) 535, 562, 603, 616
BspTNI GGTCTC 1 cut(s) 154
BsrDI GCAATG 1 cut(s) 363
BsrI ACTGG 1 cut(s) 57
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 4 cut(s) 408, 540, 554, 608
BssT1I CCWWGG 1 cut(s) 27
Bst2UI CCWGG 1 cut(s) 329
BstAPI GCANNNNNTGC 2 cut(s) 80, 650
BstC8I GCNNGC 1 cut(s) 81
BstDEI CTNAG 2 cut(s) 604, 638
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 4 cut(s) 198, 234, 388, 421
BstFNI CGCG 2 cut(s) 450, 494
BstHHI GCGC 1 cut(s) 452
BstKTI GATC 4 cut(s) 411, 543, 557, 611
BstMAI GTCTC 2 cut(s) 154, 450
BstMBI GATC 4 cut(s) 408, 540, 554, 608
BstMWI GCNNNNNNNGC 7 cut(s) 53, 62, 71, 80, 85, 650, 659
BstNI CCWGG 1 cut(s) 329
BstPAI GACNNNNGTC 1 cut(s) 297
BstSCI CCNGG 1 cut(s) 327
BstSFI CTRYAG 2 cut(s) 305, 453
BstSLI GKGCMC 1 cut(s) 76
BstUI CGCG 2 cut(s) 450, 494
BstV2I GAAGAC 1 cut(s) 278
BstX2I RGATCY 3 cut(s) 540, 554, 608
BstYI RGATCY 3 cut(s) 540, 554, 608
BsuRI GGCC 1 cut(s) 255
BtgI CCRYGG 1 cut(s) 27
BtrI CACGTC 1 cut(s) 281
BtsCI GGATG 4 cut(s) 198, 234, 388, 421
BtsI GCAGTG 2 cut(s) 48, 74
BtsIMutI CAGTG 3 cut(s) 48, 74, 176
BtuMI TCGCGA 1 cut(s) 494
Cac8I GCNNGC 1 cut(s) 81
CaiI CAGNNNCTG 2 cut(s) 68, 650
CciI TCATGA 1 cut(s) 112
CfoI GCGC 1 cut(s) 452
Cfr13I GGNCC 1 cut(s) 253
CviAII CATG 4 cut(s) 28, 113, 189, 337
CviJI RGCY 9 cut(s) 17, 56, 88, 100, 110, 255, 332, 388, 463
CviKI_1 RGCY 9 cut(s) 17, 56, 88, 100, 110, 255, 332, 388, 463
DdeI CTNAG 2 cut(s) 604, 638
DpnI GATC 4 cut(s) 410, 542, 556, 610
DpnII GATC 4 cut(s) 408, 540, 554, 608
DraIII CACNNNGTG 1 cut(s) 431
Eco130I CCWWGG 1 cut(s) 27
Eco31I GGTCTC 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 200
EcoRII CCWGG 1 cut(s) 327
EcoT14I CCWWGG 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 4 cut(s) 31, 116, 192, 340
FaqI GGGAC 1 cut(s) 596
FatI CATG 4 cut(s) 27, 112, 188, 336
Fnu4HI GCNGC 1 cut(s) 256
FokI GGATG 4 cut(s) 205, 221, 395, 428
Fsp4HI GCNGC 1 cut(s) 256
FspBI CTAG 3 cut(s) 516, 579, 654
GlaI GCGC 1 cut(s) 451
GluI GCNGC 1 cut(s) 256
GsaI CCCAGC 1 cut(s) 344
HaeIII GGCC 1 cut(s) 255
HapII CCGG 1 cut(s) 142
HhaI GCGC 1 cut(s) 452
Hin1II CATG 4 cut(s) 31, 116, 192, 340
Hin6I GCGC 1 cut(s) 450
HinP1I GCGC 1 cut(s) 450
HinfI GANTC 3 cut(s) 244, 398, 584
HpaII CCGG 1 cut(s) 142
Hpy188I TCNGA 1 cut(s) 124
Hpy188III TCNNGA 3 cut(s) 113, 142, 493
HpyAV CCTTC 3 cut(s) 139, 380, 453
HpyCH4IV ACGT 2 cut(s) 280, 376
HpyCH4V TGCA 6 cut(s) 4, 83, 307, 530, 563, 594
HpyF10VI GCNNNNNNNGC 7 cut(s) 53, 62, 71, 80, 85, 650, 659
HpyF3I CTNAG 2 cut(s) 604, 638
HpySE526I ACGT 2 cut(s) 280, 376
Hsp92II CATG 4 cut(s) 31, 116, 192, 340
HspAI GCGC 1 cut(s) 450
Kpn2I TCCGGA 1 cut(s) 141
Kzo9I GATC 4 cut(s) 408, 540, 554, 608
LmnI GCTCC 3 cut(s) 22, 53, 436
LweI GCATC 3 cut(s) 74, 172, 349
MaeI CTAG 3 cut(s) 516, 579, 654
MaeII ACGT 2 cut(s) 280, 376
MaeIII GTNAC 2 cut(s) 281, 646
MalI GATC 4 cut(s) 410, 542, 556, 610
MboI GATC 4 cut(s) 408, 540, 554, 608
MboII GAAGA 3 cut(s) 193, 238, 283
MflI RGATCY 3 cut(s) 540, 554, 608
MhlI GDGCHC 2 cut(s) 76, 441
MluCI AATT 2 cut(s) 210, 488
MlyI GAGTC 2 cut(s) 407, 578
MroI TCCGGA 1 cut(s) 141
MseI TTAA 1 cut(s) 105
MslI CAYNNNNRTG 1 cut(s) 239
MspI CCGG 1 cut(s) 142
MspR9I CCNGG 1 cut(s) 329
Mva1269I GAATGC 1 cut(s) 508
MvaI CCWGG 1 cut(s) 329
MvnI CGCG 2 cut(s) 450, 494
MwoI GCNNNNNNNGC 7 cut(s) 53, 62, 71, 80, 85, 650, 659
NcoI CCATGG 1 cut(s) 27
NdeII GATC 4 cut(s) 408, 540, 554, 608
NlaIII CATG 4 cut(s) 31, 116, 192, 340
NlaIV GGNNCC 2 cut(s) 55, 610
NmuCI GTSAC 1 cut(s) 281
NruI TCGCGA 1 cut(s) 494
PagI TCATGA 1 cut(s) 112
PctI GAATGC 1 cut(s) 508
PfeI GAWTC 1 cut(s) 244
PflMI CCANNNNNTGG 1 cut(s) 550
PkrI GCNGC 1 cut(s) 257
PleI GAGTC 2 cut(s) 406, 578
PpsI GAGTC 2 cut(s) 406, 578
PshAI GACNNNNGTC 1 cut(s) 297
Psp6I CCWGG 1 cut(s) 327
PspFI CCCAGC 1 cut(s) 340
PspGI CCWGG 1 cut(s) 327
PspN4I GGNNCC 2 cut(s) 55, 610
PspPI GGNCC 1 cut(s) 253
PstI CTGCAG 1 cut(s) 309
PstNI CAGNNNCTG 2 cut(s) 68, 650
PsuI RGATCY 3 cut(s) 540, 554, 608
RruI TCGCGA 1 cut(s) 494
RseI CAYNNNNRTG 1 cut(s) 239
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 1 cut(s) 256
Sau3AI GATC 4 cut(s) 408, 540, 554, 608
Sau96I GGNCC 1 cut(s) 253
SchI GAGTC 2 cut(s) 407, 578
ScrFI CCNGG 1 cut(s) 329
SduI GDGCHC 2 cut(s) 76, 441
SetI ASST 9 cut(s) 19, 150, 283, 292, 379, 390, 576, 600, 671
SfaNI GCATC 3 cut(s) 74, 172, 349
SfcI CTRYAG 2 cut(s) 305, 453
SmiMI CAYNNNNRTG 1 cut(s) 239
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 2 cut(s) 210, 488
SsiI CCGC 2 cut(s) 161, 256
SspMI CTAG 3 cut(s) 516, 579, 654
StyD4I CCNGG 1 cut(s) 327
StyI CCWWGG 1 cut(s) 27
TaiI ACGT 2 cut(s) 283, 379
TaqI TCGA 1 cut(s) 36
TasI AATT 2 cut(s) 210, 488
TauI GCSGC 1 cut(s) 258
TfiI GAWTC 1 cut(s) 244
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TscAI CASTG 3 cut(s) 48, 81, 183
TseFI GTSAC 1 cut(s) 281
Tsp45I GTSAC 1 cut(s) 281
TspDTI ATGAA 4 cut(s) 27, 239, 257, 325
TspGWI ACGGA 1 cut(s) 227
TspRI CASTG 3 cut(s) 48, 81, 183
Van91I CCANNNNNTGG 1 cut(s) 550
XapI RAATTY 1 cut(s) 488
XspI CTAG 3 cut(s) 516, 579, 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.